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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

MIEN1 Gene

protein-coding   GIFtS: 46
GCID: GC17M037886

migration and invasion enhancer 1

(Previous name: chromosome 17 open reading frame 37 )
(Previous symbol: C17orf37)
 Explore 6 diseases affiliated with
MIEN1 via our new
 Human Malady Compendium 
Biological research products
for MIEN1
    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section

Aliases
Migration And Invasion Enhancer 11 2     HBV XAg-Transactivated Protein 42 3
C17orf371 2 3 5     MGC148321
XTP41 2 3     Rdx121
RDX122 3 5     Chromosome 17 Open Reading Frame 371
C351 2     Protein C17orf372
ORB31 2     Protein C353
HBV X-Transactivated Gene 4 Protein2 3     

External Ids:    HGNC: 282301   Entrez Gene: 842992   Ensembl: ENSG000001417417   OMIM: 6118025   UniProtKB: Q9BRT33   

Export aliases for MIEN1 gene to outside databases


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: MIEN1_HUMAN, Q9BRT3
Function: Increases cell migration by inducing filopodia formation at the leading edge of migrating cells. Plays a role
in regulation of apoptosis, possibly through control of CASP3. May be involved in a redox-related process




(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000017.10  NC_018928.1  NT_010783.15  
Regulatory elements:
   Search SABiosciences Regulatory transcription factor binding sites for MIEN1
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidMIEN1 promoter sequence
   Search SABiosciences Chromatin IP Primers for MIEN1

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat MIEN1


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 17q12   Ensembl cytogenetic band:  17q12   HGNC cytogenetic band: 17q12

MIEN1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
MIEN1 gene location

GeneLoc information about chromosome 17         GeneLoc Exon Structure

GeneLoc location for GC17M037886:  view genomic region     (about GC identifiers)

Start:
37,884,749 bp from pter      End:
37,887,040 bp from pter
Size:
2,292 bases      Orientation:
minus strand

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: MIEN1_HUMAN, Q9BRT3 (See protein sequence)
Recommended Name: Migration and invasion enhancer 1 precursor  
Size: 115 amino acids; 12403 Da
Subunit: Interacts with GPX1 (By similarity)
Subcellular location: Cytoplasm, cytosol. Cell membrane; Lipid-anchor; Cytoplasmic side. Note=Concentrates at the
leading edge of migrating cells. Localizes outside membrane raft regions
1 PDB 3D structure from and Proteopedia for MIEN1:
2LJK (3D)    

Explore the universe of human proteins at neXtProt for MIEN1: NX_Q9BRT3

Post-translational modifications:

  • Isoprenylation facilitates association with the plasma membrane and enhances the migratory phenotype of cells by
  • inducing increased filopodia formation1
  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_Q9BRT3

  • MIEN1 Protein expression data from MOPED and PaxDb:    About this image 
    Estimated protein expression log10 (pmol).

    REFSEQ proteins: NP_115715.3  
    ENSEMBL proteins: 
     ENSP00000377778   ENSP00000462998  

    Human Recombinant Protein Products: 
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    Novus Biologicals MIEN1 Protein
    Novus Biologicals MIEN1 Lysates
    Browse Sino Biological Recombinant Proteins
    Browse ProSpec Recombinant Proteins
    Uscn Proteins for MIEN1

    Gene Ontology (GO): 2 cellular component terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005829cytosol IDA--
    GO:0031235intrinsic to internal side of plasma membrane IDA--


    MIEN1 for ontologies           About GeneDecksing



    MIEN1 Antibody Products: 
    EMD Millipore Mono- and Polyclonal Antibodies for the study of MIEN1
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    Uscn Antibodies for MIEN1
    ThermoFisher Antibodies for MIEN1

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    Uscn ELISAs and CLIAs for MIEN1


    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    MIEN1 for domains           About GeneDecksing

    2 InterPro domains/families:
     IPR012336 Thioredoxin-like_fold
     IPR011893 Selenoprotein_Rdx-typ

    Graphical View of Domain Structure for InterPro Entry Q9BRT3

    ProtoNet protein and cluster: Q9BRT3

    UniProtKB/Swiss-Prot: MIEN1_HUMAN, Q9BRT3
    Similarity: Belongs to the SelWTH family


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section

    Function Summary:

         UniProtKB/Swiss-Prot: MIEN1_HUMAN, Q9BRT3
    Function: Increases cell migration by inducing filopodia formation at the leading edge of migrating cells. Plays a role
    in regulation of apoptosis, possibly through control of CASP3. May be involved in a redox-related process

    miRNA
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    SwitchGear 3'UTR luciferase reporter plasmidMIEN1 3' UTR sequence
    Inhib. RNA
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    OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for MIEN1 (see all 4)
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    In Situ Assay
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    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for MIEN1

    Gene Ontology (GO): 2 molecular function terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005515protein binding ----
    GO:0008430selenium binding IEA--


    MIEN1 for ontologies           About GeneDecksing


    1 GenomeRNAi human phenotype for MIEN1:
     Decreased nuclei size in G2M 


    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section



    Interactions:

        Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for MIEN1

    Gene Ontology (GO): 5 biological process terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006915apoptotic process IEA--
    GO:0030335positive regulation of cell migration IDA--
    GO:0043066negative regulation of apoptotic process IDA--
    GO:0045454cell redox homeostasis IEA--
    GO:0051491positive regulation of filopodium assembly IDA--


    MIEN1 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section
    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for MIEN1
    Search CenterWatch for drugs/clinical trials and news about MIEN1 

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for MIEN1 gene: 
    NM_032339.3  

    Unigene Cluster for MIEN1:

    Migration and invasion enhancer 1
    Hs.333526  [show with all ESTs]
    Unigene Representative Sequence: BC063395
    6 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000582963 ENST00000469568 ENST00000474210 ENST00000394231(uc002hsq.3)
    ENST00000577810 ENST00000498164

    miRNA
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    QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat MIEN1
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    SwitchGear 3'UTR luciferase reporter plasmidMIEN1 3' UTR sequence
    Inhib. RNA
    Products:
         
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    OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for MIEN1 (see all 4)
    OriGene shRNA RFP: MIEN1
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    QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat MIEN1
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    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat MIEN1 
    Primer
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    Browse OriGene validated miRNA SYBR primer pairs
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      QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat MIEN1
      QIAGEN QuantiFast Probe-based Assays in human, mouse, rat MIEN1

    Additional cDNA sequence: 

    AF490253.1 AJ308025.1 AJ308026.1 AY508814.1 BC006006.1 BC063395.1 BC106016.1 

    14 DOTS entries:

    DT.450536  DT.100027600  DT.215753  DT.120977063  DT.92459289  DT.40105898  DT.100786717  DT.120977168 
    DT.95188645  DT.100786715  DT.100824047  DT.120977079  DT.91716024  DT.95197022 

    24/248 AceView cDNA sequences (see all 248):

    H96418 AA707623 AW406223 AI285284 AA284919 AA843586 BE179931 BU166943 
    BC063395 R22331 BU176101 CR613484 N42693 AI131184 AI362693 AA886218 
    CK905836 BE616135 AW009117 AF490253 BU071938 AI051009 W44577 AA978345 

    GeneLoc Exon Structure

    5 Alternative Splicing Database (ASD) splice patterns (SP) for MIEN1    About this scheme

    ExUns: 1a · 1b · 1c · 1d · 1e ^ 2 ^ 3 ^ 4a · 4b
    SP1:                          -                           
    SP2:                                -                     
    SP3:                          -                           
    SP4:                                                      
    SP5:                                                      


    ECgene alternative splicing isoforms for MIEN1

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    MIEN1 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: AACACTTCTC

    Microarray
    RNAseq (Illumina Body Map)
    (100×FPKM)½
    SAGE (Serial Analysis of Gene Expression)

    About this image
    See MIEN1 Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for MIEN1

    SOURCE GeneReport for Unigene cluster: Hs.333526

    UniProtKB/Swiss-Prot: MIEN1_HUMAN, Q9BRT3
    Tissue specificity: Among normal tissues, present only in Leydig cells. Strongly up-regulated in breast cancers and in
    brain cancer distant metastasis (at protein level). Up-regulated in prostate cancer cells and in the higher grades of
    prostate adenocarcinoma (at protein level)

        SABiosciences Custom PCR Arrays for MIEN1
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    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for MIEN1

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of animals.

    Orthologs for MIEN1 gene from 6/12 species (see all 12)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Mien11 , 5 RIKEN cDNA 1810046J19 gene5
    migration and invasion enhancer 11
    88.7(n)1
    89.57(a)1
      11 (61.75 cM)5
    1037421  NM_025559.21  NP_079835.11 
     984377085 
    chicken
    (Gallus gallus)
    Aves LOC1008582251 protein C17orf37 homolog 66.67(n)
    64(a)
      100858225  XM_003642806.1  XP_003642854.1 
    lizard
    (Anolis carolinensis)
    Reptilia MIEN16
    --
    87(a)
    1 ↔ 1
    6(68408245-68410275)
    tropical clawed frog
    (Xenopus tropicalis)
    Amphibia Str.155982 Transcribed sequence with moderate similarity to protein more 81.12(n)    142016167 
    zebrafish
    (Danio rerio)
    Actinopterygii sepw2a2 selenoprotein W, 2a 76.91(n)   378437  AY221261.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta CG154566
    --
    34(a)
    1 ↔ 1
    X(20297536-20298104)


    ENSEMBL Gene Tree for MIEN1 (if available)
    TreeFam Gene Tree for MIEN1 (if available) 

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
      --

    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/64 NCBI SNPs in MIEN1 are shown (see all 64    About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 17 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs42526661,2
    C--37885527(+) GCTTTC/TGTGGG 1 -- ut31 ese33Minor allele frequency- T:0.01NS NA 184
    rs1429978271,2
    --37885528(+) CTTTCA/GTGGGC 1 -- ut310--------
    rs1506958081,2
    --37885532(+) CGTGGA/GCACTT 1 -- ut310--------
    rs1399364171,2
    --37885558(+) TATGAA/GGTGGC 1 -- ut310--------
    rs770528761,2
    C,--37885638(+) TACCCA/GAAGGG 1 -- ut310--------
    rs345036751,2
    C,--37885640(+) CCAAA-/GGGGCA 1 -- ut310--------
    rs748459951,2
    C,--37885640(+) CCCAAG/AGGGCA 1 -- ut311Minor allele frequency- A:0.50CSA 2
    rs42526671,2
    C,F--37885641(+) CAAAG-/GGGCAA 1 -- ut312Minor allele frequency- G:0.10NS NA 180
    rs1855342691,2
    C,--37885742(+) GACCCC/TAGAAC 1 -- ut310--------
    rs2018573341,2
    --37885882(+) AGGATC/GATGCA 1 -- int10--------

    HapMap Linkage Disequilibrium report for MIEN1 (37884749 - 37887040 bp)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
          Database of Genomic Variants (DGV) variations for MIEN1: --

    SABiosciences Cancer Mutation PCR Assays
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    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    MIEN1 for disorders           About GeneDecksing

    OMIM gene information: 611802    OMIM disorders: --

    6 diseases for MIEN1:    About MalaCards
    brain cancer    breast cancer    prostate adenocarcinoma    adenocarcinoma
    prostatitis    prostate cancer

    2 Novoseek disease relationships for MIEN1 gene    About this table

    Disease   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    breast cancer 21.3 4 17121940 (2), 17117180 (1)
    tumors 0 5 17121940 (4)


    Export disorders for MIEN1 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for MIEN1 gene, integrated from 9 sources (see all 18):
    (articles sorted by number of sources associating them with MIEN1)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. C35 (C17orf37) is a novel tumor biomarker abundantly expressed in breast cancer. (PubMed id 17121940)1, 2, 3, 9 Evans E.E....Zauderer M. (2006)
    2. Prenylated c17orf37 induces filopodia formation to pr omote cell migration and metastasis. (PubMed id 21628459)1, 2, 3 Dasgupta S....Vishwanatha J.K. (2011)
    3. MGC9753 gene, located within PPP1R1B-STARD3-ERBB2-GRB7 amplicon on human chromosome 17q12, encodes the seven-transmembrane receptor with extracellular six-cystein domain. (PubMed id 12739007)1, 3, 9 Katoh M. and Katoh M. (2003)
    4. Novel gene C17orf37 in 17q12 amplicon promotes migrat ion and invasion of prostate cancer cells. (PubMed id 19503095)1, 2 Dasgupta S....Vishwanatha J.K. (2009)
    5. SelT, SelW, SelH, and Rdx12: genomics and molecular insights into the functions of selenoproteins of a novel thioredoxin-like family. (PubMed id 17503775)1, 3 Dikiy A....Gladyshev V.N. (2007)
    6. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
    7. HapMap-based study of the 17q21 ERBB2 amplicon in susceptibility to breast cancer. (PubMed id 17117180)1, 9 Benusiglio P.R....Ponder B.A. (2006)
    8. An in vitro model that recapitulates the epithelial t o mesenchymal transition (EMT) in human breast cancer. (PubMed id 21347235)1 Katz E....Harrison D.J. (2011)
    9. An analytical approach differentiates between individ ual and collective cancer invasion. (PubMed id 21483102)1 Katz E....Harrison D.J. (2011)
    10. Initial characterization of the human central proteome. (PubMed id 21269460)2 Burkard T.R.... Colinge J. (2011)

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section
     ANDOR
    Aliases
    Disorders
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 84299 HGNC: 28230 AceView: C17orf37 Ensembl:ENSG00000141741 euGenes: HUgn84299
    ECgene: MIEN1 H-InvDB: MIEN1

    (According to HUGE)
    About This Section
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for MIEN1 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
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    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or Enzo Life Sciences),
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     MIEN1 Proteins, Antibodies, CLIAs, and ELISAs
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    GeneCards Homepage - Last full update: 19 Mar 2013 - Incrementals: 21 Mar 2013 , 15 Apr 2013 , 26 Apr 2013

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