MIDN Gene
protein-coding GIFtS: 46
GCID: GC19P001248
|
|
midnolin
| |
Aliases
for MIDN gene
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc,
7Ensembl,
8DME,
9miRBase,
and/or 10fRNAdb)
About This Section
|
| Aliases |
|---|
| Midnolin1 | | Midbrain Nucleolar Protein2 3 |
Export aliases for MIDN gene to outside databasesPrevious GC identifers: GC19U990284 GC19P001319 GC19P001190 GC19P001201 GC19P001199 GC19P001020 |
Summaries
for MIDN gene(According to Entrez Gene,
Tocris Bioscience,
Wikipedia's
Gene Wiki,
PharmGKB,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
|
UniProtKB/Swiss-Prot: MIDN_HUMAN, Q504T8Function: May be involved in regulation of genes related to neurogenesis in the nucleolus (By similarity)
|
Genomic Views
for MIDN gene
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 69),
Regulatory elements and Epigenetics data according to
QIAGEN,
SABiosciences, and/or
SwitchGear Genomics)
About This Section
| RefSeq DNA sequence:- NC_000019.9 NC_018930.1 NT_011255.14
Regulatory elements:
 SABiosciences Regulatory transcription factor binding sites in the MIDN gene promoter:
Pax-5 Brachyury Lmo2 Nkx2-5 NF-kappaB E47 AREB6 ZID NF-kappaB1
Other transcription factors
Search SABiosciences Chromatin IP Primers for MIDN
Epigenetics:
| | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat MIDN |
Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track
Entrez Gene cytogenetic band: 19p13.3 Ensembl cytogenetic band: 19p13.3 HGNC chromosome: 19MIDN Gene in genomic location: bands according to Ensembl, locations according to
 (and/or Entrez Gene and/or Ensembl if different)
GeneLoc information about chromosome 19 GeneLoc Exon Structure GeneLoc location for GC19P001248: view genomic region
(about GC identifiers)
Start:
|
1,248,552 bp from pter |
End:
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1,259,142 bp from pter |
Size:
|
10,591 bases |
Orientation:
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plus strand |
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Proteins
for MIDN gene
(According to
1UniProtKB,
HORDE,
neXtProt,
Ensembl,
and/or Reactome,
Modification sites according to 2PhosphoSitePlus,
Specific Peptides from DME,
Protein expression images according to data from SPIRE MOPED and PaxDb,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from
EMD Millipore,
R&D Systems,
GenScript,
Enzo Life Sciences,
OriGene,
Novus Biologicals,
Sino Biological,
ProSpec, and/or
Uscn,
Biochemical Assays by
EMD Millipore,
R&D Systems,
OriGene,
GenScript,
Cell Signaling Technology,
Enzo Life Sciences, and/or
Uscn,
Ontologies according to Gene
Ontology Consortium 01 Mar 2013 and
Entrez Gene,
Antibodies by
EMD Millipore,
R&D Systems,
GenScript,
Cell Signaling Technology,
OriGene,
Novus Biologicals,
Thermo Fisher Scientific,
Abcam, and/or
Uscn)
About This Section
|
UniProtKB/Swiss-Prot: MIDN_HUMAN, Q504T8 (See
protein sequence)Recommended Name: Midnolin Size: 468 amino acids; 49213 Da
Subcellular location: Nucleus, nucleolus (By similarity)
Secondary accessions: Q96BW8Explore the universe of human proteins at neXtProt for MIDN: NX_Q504T8
Post-translational modifications:
View modification sites using PhosphoSitePlus2View neXtProt modification sites for NX_Q504T8
MIDN Protein expression data from MOPED and PaxDb: About this image 
REFSEQ proteins: NP_796375.3
ENSEMBL proteins: ENSP00000466895 ENSP00000467679 ENSP00000466835 ENSP00000300952
Human Recombinant Protein Products:
Gene Ontology (GO): 1 cellular component term (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0005730 | nucleolus |
IEA | -- |
MIDN for ontologies About GeneDecksing
MIDN Antibody Products:
Assay Products for MIDN: |
Protein
Domains /
Families
for MIDN gene(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
|
MIDN for domains About GeneDecksing
2 InterPro domains/families:Graphical View of Domain Structure for InterPro Entry Q504T8ProtoNet protein and cluster: Q504T8 1 Blocks protein family: IPB000626 Ubiquitin domain
UniProtKB/Swiss-Prot: MIDN_HUMAN, Q504T8Similarity: Contains 1 ubiquitin-like domain |
Function
for MIDN gene
(According to 1UniProtKB,
Genatlas,
LifeMap Discovery™,
IUBMB, and/or
2DME,
Human phenotypes from GenomeRNAi,
Animal models from MGI Mar 06 2013,
bound targets from SABiosciences,
miRNA Gene Targets from miRTarBase
shRNA from
OriGene,
RNAi from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
microRNA from QIAGEN,
Gene Editing from DNA2.0,
Clones from EMD Millipore,
OriGene,
SwitchGear Genomics,
GenScript,
Sino Biological,
DNA2.0,
and Vector BioLabs,
Cell Lines from GenScript,
LifeMap BioReagents,
In Situ Hybridization Assays from Advanced Cell Diagnostics,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene.)
About This Section
|
Function Summary:
UniProtKB/Swiss-Prot: MIDN_HUMAN, Q504T8Function: May be involved in regulation of genes related to neurogenesis in the nucleolus (By similarity)
Clone
Products: |  | Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore | |  | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for MIDN (see all 2)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for MIDN
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling 
| |  | GenScript: all cDNA clones in your preferred vector: MIDN (NM_177401) | |  | Browse Sino Biological Human cDNA Clones | |  | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for MIDN | |  | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat MIDN |
In Situ Assay
Products: |
 | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for MIDN |
|
Pathways & Interactions
for MIDN gene
(Pathways according to
EMD Millipore,
R&D Systems,
Cell Signaling Technology,
KEGG,
PharmGKB,
BioSystems,
Reactome,
Tocris Bioscience,
GeneGo (Thomson Reuters),
QIAGEN,
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Interaction Networks according to
SABiosciences,
and/or STRING,
Interactions according to 1UniProtKB,
2MINT,
3I2D, and/or
4STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene).
About This Section
|
Interactions:
Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for MIDN
STRING Interaction
Network Preview (showing 2 interactants - click image to see more details)
5/7 Interacting proteins for MIDN (Q504T82, 3 ENSP000003009524) via UniProtKB, MINT, STRING, and/or I2D (see all 7)About this table
|
Drugs & Compounds
for MIDN gene(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
EMD Millipore, Tocris Bioscience
HMDB,
BitterDB, and/or
Novoseek, and Drugs according to
DrugBank,
Enzo Life Sciences, and/or
PharmGKB, with drugs/clinical trials/news
search links to CenterWatch)
About This Section
|
 Browse Tocris compounds for MIDN
Search CenterWatch for drugs/clinical trials and news about MIDN
|
Transcripts
for MIDN gene(Secondary structures according to
fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
transcript ids from Ensembl
with links to UCSC,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
shRNA from
OriGene,
microRNA from QIAGEN,
Tagged/untagged cDNA clones from
OriGene,
SwitchGear Genomics,
GenScript,
DNA2.0,
Vector BioLabs,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN
)
About This Section
|
REFSEQ mRNAs for MIDN gene: NM_177401.4 Unigene Cluster for MIDN: Midnolin Hs.465529 [show with all ESTs]Unigene Representative Sequence: NM_1774016 Ensembl transcripts including schematic representations, and UCSC links where relevant: ENST00000586757 ENST00000591446 ENST00000591302 ENST00000586843 ENST00000590136
ENST00000300952(uc002lrp.3)
Clone
Products: | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for MIDN (see all 2)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for MIDN
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling
| | | GenScript: all cDNA clones in your preferred vector: MIDN (NM_177401) | | | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for MIDN | | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat MIDN |
Additional cDNA sequence: AK075506.1 AL512725.1 BC015089.2 BC060848.1 BC094778.1 17 DOTS entries: DT.450577 DT.97864632 DT.100779547 DT.428255 DT.91859455 DT.100035137 DT.100779545 DT.40273416 DT.40288563 DT.91676416 DT.100779551 DT.95125822 DT.95198507 DT.95362765 DT.91746056 DT.91874872 DT.95272678 24/278 AceView cDNA sequences (see all 278): BU069685 AA907426 AI918888 AI540078 AI918164 AI079230 BQ890372 AI870830 AI568750 BE855711 AI239624 AW135403 BM718160 BM893537 AA483833 AI393265 AI288226 AI697027 CA771108 AI581091 CB216641 AW016647 BU741795 AI300085
GeneLoc Exon Structure
|
Expression
for MIDN gene
(RNA expression data according to
H-InvDB,
NONCODE,
miRBase, and
RNAdb,
Expression images according to data from
BioGPS,
Illumina Human BodyMap, and
CGAP
SAGE,
Sets of similar genes according to GeneDecks,
in vivo and in vitro expression data from LifeMap Discovery™,
plus additional links to
Genevestigator, and/or
SOURCE, and/or
BioGPS, and/or
UniProtKB,
PCR Arrays from
SABiosciences,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN,
In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section
| MIDN expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: TATGAAAACA
About this image
See MIDN Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for MIDN
SOURCE GeneReport for Unigene cluster: Hs.465529
SABiosciences Custom PCR Arrays for MIDN
Primer
Products: | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for MIDN
Browse OriGene validated miRNA SYBR primer pairs
| | | SABiosciences RT2 qPCR Primer Assay in human, mouse, rat MIDN | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat MIDN | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat MIDN | In Situ
Assay Products: |
| Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for MIDN |
Orthologs
for MIDN gene
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
,
5MGI Mar 06 2013,
with possible further links to
Flybase
and/or
WormBase,
and/or
6Ensembl pan taxonomic compara ,
Gene Trees according to Ensembl and
TreeFam)
About This Section
|
This gene was present in the common ancestor of animals.
Orthologs for MIDN gene from 5/15 species (see all 15) About this table
| Organism |
Taxonomic
classification |
Gene |
Description |
Human
Similarity |
Orthology
Type |
Details |
mouse
(Mus musculus) |
Mammalia |
Midn1 , 5 |
midnolin1, 5 |
84.7(n)1
94.16(a)1 |
|
10 (39.72 cM)5
590901 NM_021565.11 NP_067540.11
801482725 |
lizard
(Anolis carolinensis) |
Reptilia |
MIDN6 |
-- |
63(a) |
1 ↔ 1 |
GL344335.1(1510-7093) |
African clawed frog
(Xenopus laevis) |
Amphibia |
MGC528972 |
similar to midnolin |
74.55(n) |
|
BC046658.1 |
zebrafish
(Danio rerio) |
Actinopterygii |
wufc07f112 |
Transcribed sequence with weak similarity to protein more |
79.37(n) |
|
CA474471.1 |
fruit fly
(Drosophila melanogaster) |
Insecta |
CG326766 |
-- |
14(a) |
1 ↔ 1 |
X(10605196-10638938) |
ENSEMBL Gene Tree for MIDN (if available)
TreeFam Gene Tree for MIDN (if available) |
Paralogs
for MIDN gene(Paralogs according to
1HomoloGene,
2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
About This Section
| -- |
Genomic Variants
for MIDN gene(SNPs/Variants according to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE,
UniProtKB, and
DNA2.0,
Linkage Disequilibrium by HapMap,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene
Mutation Database (HGMD) and the Locus Specific Mutation
Databases (LSDB), Blood group antigen gene mutations by BGMUT,
Resequencing Primers from QIAGEN,
Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section
|
| Genomic Data | Transcription Related Data | Allele Frequencies | | SNP ID | Valid | Clinical
significance | Chr 19 pos | Sequence | # | AA
Chg | Type | More | # | Allele
freq | Pop | Total
sample | More |
|---|
HapMap Linkage Disequilibrium report for MIDN (1248552 - 1259142 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV): 6 variations for MIDN
6 CNVs: 73274 30057 30058 4066 29684 30885 | SABiosciences Cancer Mutation PCR Assays |
| | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing MIDN |
|
Disorders
/ Diseases
for MIDN gene
(in which this Gene is Involved, According to MalaCards,
OMIM, UniProtKB,
the University of Copenhagen DISEASES
database,
Genatlas, GeneTests,
GAD,
HuGE Navigator,
and/or TGDB.)
About This Section
|
MIDN for disorders About GeneDecksing
OMIM gene information: 606700
OMIM disorders: --
1  disease for MIDN: About MalaCardspeutz-jeghers syndrome
1 disease from the University of Copenhagen DISEASES database for MIDN:Peutz-Jeghers syndrome Export disorders for MIDN gene to outside databases
|
Publications
for MIDN gene (in
PubMed.
Associations of this gene to articles via
1Entrez Gene,
2UniProtKB/Swiss-Prot,
3HGNC,
4GAD,
5PharmGKB,
6HMDB,
7DrugBank,
8UniProtKB/TrEMBL,
9 Novoseek, and/or
10fRNAdb)
About This Section
|
PubMed articles for MIDN gene integrated from 9 sources:
(articles sorted by number of sources associating them with MIDN) | |  | Utopia: connect your pdf to the dynamic
world of online information |
- Novel nucleolar protein, midnolin, is expressed in the mesencephalon during mouse development. (PubMed id 10974535)1, 3 Tsukahara M....Tsunoo H. (2000)
- Methods for quantification of in vivo changes in prote in ubiquitination following proteasome and deubiquitinase inhibition. (PubMed id 22505724)1 Udeshi N.D....Carr S.A. (2012)
- Systematic and quantitative assessment of the ubiquiti n-modified proteome. (PubMed id 21906983)1 Kim W....Gygi S.P. (2011)
- A directed protein interaction network for investigat ing intracellular signal transduction. (PubMed id 21900206)1 Vinayagam A....Wanker E.E. (2011)
- Ubiquitin-mediated proteolysis of HuR by heat shock. (PubMed id 19322201)1 Abdelmohsen K....Gorospe M. (2009)
- Signal sequence and keyword trap in silico for selection of full- length human cDNAs encoding secretion or membrane proteins from oligo- capped cDNA libraries. (PubMed id 16303743)1 Otsuki T....Isogai T. (2005)
- The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)2 Gerhard D.S....Malek J. (2004)
- Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1 Strausberg R.L....Marra M.A. (2002)
- Human chromosome 19 and related regions in mouse: con servative and lineage-specific evolution. (PubMed id 11441184)1 Dehal P....Stubbs L. (2001)
|
External Searches for MIDN gene
(in PubMed,
OMIM, and NCBI Bookshelf)
About This Section
|
|
Genome Databases showing MIDN gene
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
Kegg,
and/or
H-InvDB)
About This Section
|
|
Other Databases showing MIDN gene
(According to HUGE)
About This Section
| -- |
Specialized Databases showing MIDN gene(According to PharmGKB,
ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
|
| Name | Description |
| PharmGKB entry for MIDN | Pharmacogenomics, SNPs, Pathways |
|
| | |
About This Section
| Patent Information for MIDN gene:
Search GeneIP for patents involving MIDN
GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
|
Products
for MIDN gene(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or
Enzo Life Sciences),
In Situ Hybridization Assays from
Advanced Cell Diagnostics
About This Section
|
 | |
 | |
 |
| | |  | Browse OriGene Antibodies | | OriGene shRNA RFP for MIDN | | | OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for MIDN | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for MIDN | | | OriGene Protein Over-expression Lysate for MIDN | | Browse OriGene Fluorogenic Cell Assay Kits | | | OriGene siRNA for MIDN | | Browse 3'-UTR reporter clones for miRNA target validation | | | OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for MIDN | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for MIDN | | | Browse OriGene GFP tagged cDNA clones in CMV expression vector | | Browse OriGene MicroRNA Expression Plasmids | | | Browse OriGene basic RS shRNAs | | Browse OriGene validated miRNA SYBR primer pairs | | | Browse OriGene full length recombinant human proteins expressed in human HEK293 cells | | OriGene custom cloning services - gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling | | | OriGene Custom Antibody Services for MIDN | | OriGene Custom Protein Services for MIDN | | | OriGene Custom Immunoassay Development | | |
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 |
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| | | QIAGEN Custom miScript Target Protector blocks miRNA-binding site of in human, mouse, rat MIDN | | | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing MIDN | | | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat MIDN | | | QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat MIDN | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat MIDN | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat MIDN |
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 |  |  |  | |
| | | | Search Tocris compounds for MIDN |
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 | | | MIDN Proteins, Antibodies, CLIAs, and ELISAs |
| | | | | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for MIDN |
|  |  |  | | | | Search ThermoFisher Antibodies for MIDN |
| | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat MIDN |
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