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Aliases for MID2 Gene

Aliases for MID2 Gene

  • Midline 2 2 3
  • Tripartite Motif-Containing Protein 1 3 4
  • RING Finger Protein 60 3 4
  • Midline Defect 2 3 4
  • Midline-2 3 4
  • Midin-2 3 4
  • RNF60 3 4
  • TRIM1 3 4
  • FXY2 3 4
  • Probable E3 Ubiquitin-Protein Ligase MID2 3
  • Tripartite Motif Protein 1 3
  • EC 6.3.2.- 4
  • Midin 2 3
  • MRX101 3

External Ids for MID2 Gene

Previous GeneCards Identifiers for MID2 Gene

  • GC0XP102390
  • GC0XP103988
  • GC0XP105102
  • GC0XP105841
  • GC0XP106875
  • GC0XP106955
  • GC0XP107068
  • GC0XP096692

Summaries for MID2 Gene

Entrez Gene Summary for MID2 Gene

  • The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to microtubular structures in the cytoplasm. Alternate splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Feb 2009]

GeneCards Summary for MID2 Gene

MID2 (Midline 2) is a Protein Coding gene. Diseases associated with MID2 include mental retardation, x-linked 101 and non-syndromic x-linked intellectual disability. GO annotations related to this gene include ligase activity. An important paralog of this gene is MID1.

UniProtKB/Swiss-Prot for MID2 Gene

  • May play a role in microtubule stabilization.

Gene Wiki entry for MID2 Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for MID2 Gene

Genomics for MID2 Gene

Regulatory Elements for MID2 Gene

Genomic Location for MID2 Gene

107,825,663 bp from pter
107,931,637 bp from pter
105,975 bases
Plus strand

Genomic View for MID2 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for MID2 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for MID2 Gene

Proteins for MID2 Gene

  • Protein details for MID2 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Recommended name:
    Probable E3 ubiquitin-protein ligase MID2
    Protein Accession:
    Secondary Accessions:
    • A6NEL8
    • A6PVI5
    • Q5JYF5
    • Q8WWK1
    • Q9UJR9

    Protein attributes for MID2 Gene

    735 amino acids
    Molecular mass:
    83210 Da
    Quaternary structure:
    • Homodimer or heterodimer with MID1. Interacts with IGBP1.
    • Sequence=AAF07341.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305}; Sequence=AAH17707.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305}; Sequence=CAB56154.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305}; Sequence=CAI42073.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305}; Sequence=CAO72053.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};

    Three dimensional structures from OCA and Proteopedia for MID2 Gene

    Alternative splice isoforms for MID2 Gene


neXtProt entry for MID2 Gene

Proteomics data for MID2 Gene at MOPED

Post-translational modifications for MID2 Gene

Other Protein References for MID2 Gene

Antibody Products

No data available for DME Specific Peptides for MID2 Gene

Domains for MID2 Gene

Gene Families for MID2 Gene

  • FN3 :Fibronectin type III domain containing
  • RNF :RING-type (C3HC4) zinc fingers
  • TRIM :Tripartite motif containing / Tripartite motif containing

Suggested Antigen Peptide Sequences for MID2 Gene

Graphical View of Domain Structure for InterPro Entry



  • Q9UJV3
  • The tripartite motif (RBCC; RING- and B box-type zinc fingers and coiled coil domains) mediates dimerization.
  • Associates with microtubules in a manner that is dependent on the C-terminal B30.2 domain
  • Contains 1 B30.2/SPRY domain.
  • Contains 1 COS domain.
  • Contains 1 fibronectin type-III domain.
  • Belongs to the TRIM/RBCC family.
  • Contains 2 B box-type zinc fingers.
  • Contains 1 RING-type zinc finger.
genes like me logo Genes that share domains with MID2: view

Function for MID2 Gene

Molecular function for MID2 Gene

GENATLAS Biochemistry:
midline 2,highly homolog to MID1,expressed in fetal tissues noteworthy in developing heart,confined to the cytoplasm in association to microtubule structures
UniProtKB/Swiss-Prot Function:
May play a role in microtubule stabilization.

Enzyme Numbers (IUBMB) for MID2 Gene

Gene Ontology (GO) - Molecular Function for MID2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005515 protein binding IPI 25416956
GO:0008017 microtubule binding IMP 11806752
GO:0008270 zinc ion binding IEA --
GO:0016874 ligase activity IEA --
GO:0042803 protein homodimerization activity IDA 11806752
genes like me logo Genes that share ontologies with MID2: view

Phenotypes for MID2 Gene

genes like me logo Genes that share phenotypes with MID2: view

miRNA for MID2 Gene

miRTarBase miRNAs that target MID2

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for MID2

In Situ Assay Products

Flow Cytometry Products

No data available for Animal Models , Transcription Factor Targeting and HOMER Transcription for MID2 Gene

Localization for MID2 Gene

Subcellular locations from UniProtKB/Swiss-Prot for MID2 Gene

Cytoplasm. Cytoplasm, cytoskeleton. Note=Microtubule-associated.

Subcellular locations from

Jensen Localization Image for MID2 Gene COMPARTMENTS Subcellular localization image for MID2 gene
Compartment Confidence
cytoskeleton 5
cytosol 3
nucleus 3
endoplasmic reticulum 1
plasma membrane 1

Gene Ontology (GO) - Cellular Components for MID2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005622 intracellular --
GO:0005737 cytoplasm IEA --
GO:0005874 colocalizes_with microtubule IDA 10400986
GO:0070062 extracellular exosome IDA 19056867
genes like me logo Genes that share ontologies with MID2: view

Pathways for MID2 Gene

SuperPathways for MID2 Gene

No Data Available

PCR Array Products

  • Pathway & Disease-focused RT² Profiler PCR Arrays

UniProtKB/Swiss-Prot Q9UJV3-TRIM1_HUMAN

  • Pathway: Protein modification; protein ubiquitination

Gene Ontology (GO) - Biological Process for MID2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0016567 protein ubiquitination IEA --
GO:0032897 negative regulation of viral transcription IDA 18248090
GO:0035372 protein localization to microtubule IMP 11806752
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB signaling IDA 23077300
GO:0045087 innate immune response IDA 18248090
genes like me logo Genes that share ontologies with MID2: view

No data available for Pathways by source for MID2 Gene

Transcripts for MID2 Gene

Unigene Clusters for MID2 Gene

Midline 2:
Representative Sequences:

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for MID2

Primer Products

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for MID2 Gene

ExUns: 1 ^ 2 ^ 3 ^ 4 ^ 5 ^ 6a · 6b ^ 7 ^ 8 ^ 9
SP1: -

Relevant External Links for MID2 Gene

GeneLoc Exon Structure for
ECgene alternative splicing isoforms for

Expression for MID2 Gene

mRNA expression in normal human tissues for MID2 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Protein differential expression in normal tissues for MID2 Gene

This gene is overexpressed in Cerebrospinal fluid (47.9) and Platelet (17.2).

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, and MOPED for MID2 Gene

SOURCE GeneReport for Unigene cluster for MID2 Gene Hs.12256

mRNA Expression by UniProt/SwissProt for MID2 Gene

Tissue specificity: Low level in fetal kidney and lung, and in adult prostate, ovary and small intestine
genes like me logo Genes that share expressions with MID2: view

Expression partners for MID2 Gene

In Situ Assay Products

No data available for mRNA differential expression in normal tissues for MID2 Gene

Orthologs for MID2 Gene

This gene was present in the common ancestor of chordates.

Orthologs for MID2 Gene

Organism Taxonomy Gene Similarity Type Details
(Canis familiaris)
Mammalia MID2 35
  • 95.69 (n)
  • 98.91 (a)
MID2 36
  • 99 (a)
(Mus musculus)
Mammalia Mid2 35
  • 94.01 (n)
  • 99.27 (a)
Mid2 16
Mid2 36
  • 99 (a)
(Pan troglodytes)
Mammalia MID2 35
  • 99.82 (n)
  • 100 (a)
MID2 36
  • 100 (a)
(Rattus norvegicus)
Mammalia Mid2 35
  • 93.74 (n)
  • 98.91 (a)
(Bos Taurus)
Mammalia -- 36
  • 99 (a)
-- 36
  • 99 (a)
(Monodelphis domestica)
Mammalia MID2 36
  • 94 (a)
(Ornithorhynchus anatinus)
Mammalia MID2 36
  • 93 (a)
(Gallus gallus)
Aves MID2 35
  • 81.27 (n)
  • 93.14 (a)
MID2 36
  • 91 (a)
(Anolis carolinensis)
Reptilia MID2 36
  • 88 (a)
tropical clawed frog
(Silurana tropicalis)
Amphibia LOC100497646 35
  • 75.54 (n)
  • 83.63 (a)
(Danio rerio)
Actinopterygii mid2 36
  • 83 (a)
MID2 (2 of 2) 36
  • 72 (a)
sea squirt
(Ciona savignyi)
Ascidiacea -- 36
  • 41 (a)
Species with no ortholog for MID2:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for MID2 Gene

Gene Tree for MID2 (if available)
Gene Tree for MID2 (if available)

Paralogs for MID2 Gene

Selected SIMAP similar genes for MID2 Gene using alignment to 2 proteins:

genes like me logo Genes that share paralogs with MID2: view

Variants for MID2 Gene

Sequence variations from dbSNP and Humsavar for MID2 Gene

SNP ID Clin Chr 0X pos Sequence Context AA Info Type MAF
rs1008503 -- 107,831,270(+) TTCCC(A/G)TAGGT intron-variant
rs2236083 -- 107,840,845(+) GTATC(A/C)AGCTG reference, synonymous-codon
rs2267975 -- 107,865,573(-) AGAAC(A/C)CTTTG intron-variant
rs2273377 -- 107,854,835(+) AGTCT(C/T)ATTCC intron-variant
rs2300103 -- 107,867,978(-) TTATG(A/T)CTATC intron-variant

Structural Variations from Database of Genomic Variants (DGV) for MID2 Gene

Variant ID Type Subtype PubMed ID
nsv508789 CNV Insertion 20534489

Relevant External Links for MID2 Gene

HapMap Linkage Disequilibrium report
Human Gene Mutation Database (HGMD)
Locus Specific Mutation Databases (LSDB)

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for MID2 Gene

Disorders for MID2 Gene

MalaCards: The human disease database

MalaCards: The human disease database.

Search for MID2 Gene in MalaCards »

(2) Diseases for MID2 Gene including...


  • Mental retardation, X-linked 101 (MRX101) [MIM:300928]: A disorder characterized by significantly below average general intellectual functioning associated with impairments in adaptive behavior and manifested during the developmental period. Intellectual deficiency is the only primary symptom of non-syndromic X-linked mental retardation, while syndromic mental retardation presents with associated physical, neurological and/or psychiatric manifestations. MRX101 clinical features include global developmental delay, hyperactivity often with aggressive outbursts, and seizures in some patients. Several affected individuals have long face, prominent ears, and squint or strabismus. {ECO:0000269 PubMed:24115387}. Note=The disease is caused by mutations affecting the gene represented in this entry.

(2) Novoseek inferred disease relationships for MID2 Gene

Disease -log(P) Hits PubMed IDs
hypospadias 78.7 2
developmental disabilities 49.1 2

Relevant External Links for MID2

Genetic Association Database (GAD)
genes like me logo Genes that share disorders with MID2: view

No data available for OMIM , University of Copenhagen DISEASES and Genatlas for MID2 Gene

Publications for MID2 Gene

  1. MID2, a homologue of the Opitz syndrome gene MID1: similarities in a sub-cellular localization and differences in expression during development. (PMID: 10400986) Buchner G. … Franco B. (Hum. Mol. Genet. 1999) 2 3 4 23
  2. FXY2/MID2, a gene related to the X-linked Opitz syndrome gene FXY/MID1, maps to Xq22 and encodes a FNIII domain-containing protein that associates with microtubules. (PMID: 10644436) Perry J. … Ashworth A. (Genomics 1999) 3 4 23
  3. MID1 and MID2 homo- and heterodimerise to tether the rapamycin- sensitive PP2A regulatory subunit, Alpha 4, to microtubules: implications for the clinical variability of X-linked Opitz GBBB syndrome and other developmental disorders. (PMID: 11806752) Short K.M. … Cox T.C. (BMC Cell Biol. 2002) 3 4 23
  4. Subclassification of the RBCC/TRIM superfamily reveals a novel motif necessary for microtubule binding. (PMID: 16434393) Short K.M. … Cox T.C. (J. Biol. Chem. 2006) 3 23
  5. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PMID: 15489334) Gerhard D.S. … Malek J. (Genome Res. 2004) 3 4

Products for MID2 Gene

  • Addgene plasmids for MID2

Sources for MID2 Gene

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