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Aliases for MGMT Gene

Aliases for MGMT Gene

  • O-6-Methylguanine-DNA Methyltransferase 2 3 5
  • Methylated-DNA--Protein-Cysteine Methyltransferase 2 3
  • 6-O-Methylguanine-DNA Methyltransferase 3 4
  • O-6-Methylguanine-DNA-Alkyltransferase 3 4
  • EC 2.1.1.63 4 61
  • O6-Methylguanine-DNA Methyltransferase 3
  • Methylguanine-DNA Methyltransferase 3

External Ids for MGMT Gene

Previous GeneCards Identifiers for MGMT Gene

  • GC10P130078
  • GC10P130307
  • GC10P131195
  • GC10P130739
  • GC10P130808
  • GC10P131155
  • GC10P124842

Summaries for MGMT Gene

Entrez Gene Summary for MGMT Gene

  • Alkylating agents are potent carcinogens that can result in cell death, mutation and cancer. The protein encoded by this gene is a DNA repair protein that is involved in cellular defense against mutagenesis and toxicity from alkylating agents. The protein catalyzes transfer of methyl groups from O(6)-alkylguanine and other methylated moieties of the DNA to its own molecule, which repairs the toxic lesions. Methylation of the genes promoter has been associated with several cancer types, including colorectal cancer, lung cancer, lymphoma and glioblastoma. [provided by RefSeq, Sep 2015]

CIViC summary for MGMT Gene

GeneCards Summary for MGMT Gene

MGMT (O-6-Methylguanine-DNA Methyltransferase) is a Protein Coding gene. Diseases associated with MGMT include Malignant Glioma and Primary Central Nervous System Lymphoma. Among its related pathways are Chks in Checkpoint Regulation and DNA Double-Strand Break Repair. GO annotations related to this gene include calcium ion binding and damaged DNA binding.

UniProtKB/Swiss-Prot for MGMT Gene

  • Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.

Tocris Summary for MGMT Gene

  • Protein arginine methyltransferases are enyzmes that catalyze the transfer of methyl groups from S-adenosylmethionine (SAM) to the arginine residues on histones and other proteins. The dysregulation of this methylation is critical in the development of certain cancers.

Gene Wiki entry for MGMT Gene

No data available for PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for MGMT Gene

Genomics for MGMT Gene

Regulatory Elements for MGMT Gene

Enhancers for MGMT Gene
GeneHancer Identifier Enhancer Score Enhancer Sources Gene-Enhancer Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Gene Targets for Enhancer
GH10G129605 2.5 VISTA Ensembl ENCODE 26.1 +139.1 139121 1.5 FEZF1 ZNF2 GLIS2 ZNF143 KLF7 JUNB TSHZ1 ZNF488 TBX21 ZNF518A MGMT GC10P129600 GC10M129701
GH10G129647 1.4 VISTA 30 +181.3 181289 1.9 SMARCA4 RFX5 MGMT GC10P129600 GC10M129701
GH10G129530 0.8 Ensembl ENCODE 37.1 +64.7 64734 2.0 ELF3 TEAD4 EZH2 EGR2 TEAD3 MGMT GC10M129442 GC10P129600
GH10G129466 0.9 ENCODE 31.8 +0.3 269 2.3 FOXA2 ATF1 SREBF2 KLF17 ZNF2 ZBTB7B RAD21 YY1 EGR1 MIXL1 MGMT GC10M129442
GH10G129524 1.1 Ensembl ENCODE 19.2 +58.5 58499 1.5 ZSCAN4 INSM2 ZNF76 KLF17 FEZF1 ZNF2 ZNF366 ZSCAN5C SCRT2 ZNF391 MGMT GC10M129442 GC10P129600
- Elite enhancer and/or Elite enhancer-gene association Download GeneHancer data dump

Enhancers around MGMT on UCSC Golden Path with GeneCards custom track

Promoters for MGMT Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters
ENSR00000035230 116 1001 KLF17 ZNF2 ZBTB7B RAD21 YY1 EGR1 MIXL1 SP3 ZNF592 NR2F2

Genomic Location for MGMT Gene

Chromosome:
10
Start:
129,467,184 bp from pter
End:
129,768,042 bp from pter
Size:
300,859 bases
Orientation:
Plus strand

Genomic View for MGMT Gene

Genes around MGMT on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
MGMT Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for MGMT Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for MGMT Gene

Proteins for MGMT Gene

  • Protein details for MGMT Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P16455-MGMT_HUMAN
    Recommended name:
    Methylated-DNA--protein-cysteine methyltransferase
    Protein Accession:
    P16455
    Secondary Accessions:
    • Q5VY78

    Protein attributes for MGMT Gene

    Size:
    207 amino acids
    Molecular mass:
    21646 Da
    Cofactor:
    Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
    Quaternary structure:
    No Data Available
    Miscellaneous:
    • This enzyme catalyzes only one turnover and therefore is not strictly catalytic. According to one definition, an enzyme is a biocatalyst that acts repeatedly and over many reaction cycles.

    Three dimensional structures from OCA and Proteopedia for MGMT Gene

neXtProt entry for MGMT Gene

Selected DME Specific Peptides for MGMT Gene

P16455:
  • SFTRQVLWKLLKVVKFGE
  • SYQQLAALAGNPKAARAVGGAMR
  • KEWLLAHEG
  • PALHHPVFQQ
  • NPVPILIPCHRV

Post-translational modifications for MGMT Gene

  • Ubiquitination at posLast=125125 and posLast=178178
  • Modification sites at PhosphoSitePlus

Other Protein References for MGMT Gene

ENSEMBL proteins:
REFSEQ proteins:

Domains & Families for MGMT Gene

Suggested Antigen Peptide Sequences for MGMT Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

P16455

UniProtKB/Swiss-Prot:

MGMT_HUMAN :
  • Belongs to the MGMT family.
Family:
  • Belongs to the MGMT family.
genes like me logo Genes that share domains with MGMT: view

No data available for Gene Families for MGMT Gene

Function for MGMT Gene

Molecular function for MGMT Gene

GENATLAS Biochemistry:
O6-methylguanine-DNA-methyltransferase
UniProtKB/Swiss-Prot CatalyticActivity:
DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine.
UniProtKB/Swiss-Prot Function:
Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.

Enzyme Numbers (IUBMB) for MGMT Gene

Gene Ontology (GO) - Molecular Function for MGMT Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003677 DNA binding IEA --
GO:0003824 catalytic activity IEA --
GO:0003908 methylated-DNA-[protein]-cysteine S-methyltransferase activity IEA --
GO:0005509 calcium ion binding IEA --
GO:0008168 methyltransferase activity TAS 2188979
genes like me logo Genes that share ontologies with MGMT: view
genes like me logo Genes that share phenotypes with MGMT: view

Human Phenotype Ontology for MGMT Gene

HPO Id HPO Name Alternative Ids Definition Synonyms

Animal Models for MGMT Gene

MGI Knock Outs for MGMT:

Animal Model Products

  • Taconic Biosciences Mouse Models for MGMT

CRISPR Products

Inhibitory RNA Products

Clone Products

  • Applied Biological Materials Clones for MGMT

No data available for Transcription Factor Targets and HOMER Transcription for MGMT Gene

Localization for MGMT Gene

Subcellular locations from UniProtKB/Swiss-Prot for MGMT Gene

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for MGMT gene
Compartment Confidence
nucleus 5
extracellular 2
mitochondrion 2
cytosol 2
cytoskeleton 1
peroxisome 1

Gene Ontology (GO) - Cellular Components for MGMT Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus TAS 2188979
GO:0005654 nucleoplasm TAS --
GO:0016020 membrane IDA 19946888
genes like me logo Genes that share ontologies with MGMT: view

Pathways & Interactions for MGMT Gene

genes like me logo Genes that share pathways with MGMT: view

Pathways by source for MGMT Gene

2 Cell Signaling Technology pathways for MGMT Gene
1 R&D Systems pathway for MGMT Gene
2 Qiagen pathways for MGMT Gene

Gene Ontology (GO) - Biological Process for MGMT Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006266 DNA ligation TAS 2188979
GO:0006281 DNA repair IEA --
GO:0006306 DNA methylation IEA --
GO:0006307 DNA dealkylation involved in DNA repair TAS --
GO:0006974 cellular response to DNA damage stimulus IEA --
genes like me logo Genes that share ontologies with MGMT: view

No data available for SIGNOR curated interactions for MGMT Gene

Drugs & Compounds for MGMT Gene

(66) Drugs for MGMT Gene - From: DrugBank, ClinicalTrials, ApexBio, DGIdb, HMDB, Tocris, and Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Temozolomide Approved, Investigational Pharma Biomarker Alkylating Agents 711
L-Cysteine Approved Nutra Target 0
Dacarbazine Approved, Investigational Pharma 688
Lomustine Approved Pharma Antineoplastic drug 84
Procarbazine Approved Pharma 69

(52) Additional Compounds for MGMT Gene - From: Novoseek

Name Synonyms Role CAS Number PubChem IDs PubMed IDs

(5) Tocris Compounds for MGMT Gene

Compound Action Cas Number
5-Azacytidine DNA methyltransferase inhibitor 320-67-2
5-Iodotubercidin Potent adenosine kinase inhibitor; also inhibits nucleoside transporters and a range of other kinases 24386-93-4
Decitabine DNA methyltransferase inhibitor 2353-33-5
RG 108 Non-nucleoside DNA methyltransferase inhibitor 48208-26-0
Zebularine DNA methyltransferase and cytidine deaminase inhibitor 3690-10-6

(8) ApexBio Compounds for MGMT Gene

Compound Action Cas Number
5-Azacytidine DNA methyltransferase inhibitor. 320-67-2
Decitabine (NSC127716, 5AZA-CdR) Deoxycytidine analog and cellular diifferentiation inducer 2353-33-5
Glucose-conjugated MGMT inhibitor Glucose-conjugated MGMT inhibitor 382607-78-5
Lomeguatrib MGMT inhibitor 192441-08-0
O6-Benzylguanine Potent MGMT inhibitor 19916-73-5
RG 108 DNA methyltransferase inhibitor 48208-26-0
Thioguanine Purine antimetabolite 154-42-7
Zebularine DNA methylation inhibitor 3690-10-6
genes like me logo Genes that share compounds with MGMT: view

Drug Products

Transcripts for MGMT Gene

Unigene Clusters for MGMT Gene

O-6-methylguanine-DNA methyltransferase:
Representative Sequences:

CRISPR Products

Inhibitory RNA Products

Clone Products

  • Applied Biological Materials Clones for MGMT

Alternative Splicing Database (ASD) splice patterns (SP) for MGMT Gene

No ASD Table

Relevant External Links for MGMT Gene

GeneLoc Exon Structure for
MGMT
ECgene alternative splicing isoforms for
MGMT

Expression for MGMT Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and CGAP SAGE for MGMT Gene

mRNA differential expression in normal tissues according to GTEx for MGMT Gene

This gene is overexpressed in Liver (x5.5).

Protein differential expression in normal tissues from HIPED for MGMT Gene

This gene is overexpressed in Lymph node (13.8).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, and MOPED for MGMT Gene



Protein tissue co-expression partners for MGMT Gene

- Elite partner

NURSA nuclear receptor signaling pathways regulating expression of MGMT Gene:

MGMT

SOURCE GeneReport for Unigene cluster for MGMT Gene:

Hs.501522

Evidence on tissue expression from TISSUES for MGMT Gene

  • Liver(4.5)
  • Intestine(2.9)
  • Nervous system(2.9)
  • Lung(2.5)
  • Blood(2.2)
  • Lymph node(2.2)
  • Skin(2.1)
genes like me logo Genes that share expression patterns with MGMT: view

Primer Products

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , mRNA Expression by UniProt/SwissProt and Phenotype-based relationships between genes and organs from Gene ORGANizer for MGMT Gene

Orthologs for MGMT Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for MGMT Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia MGMT 34 35
  • 98.88 (n)
cow
(Bos Taurus)
Mammalia MGMT 34
  • 77.73 (n)
mouse
(Mus musculus)
Mammalia Mgmt 34 16 35
  • 75.57 (n)
dog
(Canis familiaris)
Mammalia MGMT 34 35
  • 75.36 (n)
rat
(Rattus norvegicus)
Mammalia Mgmt 34
  • 74.02 (n)
platypus
(Ornithorhynchus anatinus)
Mammalia MGMT 35
  • 55 (a)
OneToOne
chicken
(Gallus gallus)
Aves MGMT 34 35
  • 63.8 (n)
lizard
(Anolis carolinensis)
Reptilia MGMT 35
  • 69 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia mgmt 34
  • 60.75 (n)
Str.8455 34
zebrafish
(Danio rerio)
Actinopterygii mgmt 34 35
  • 51.9 (n)
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes MGT1 37
Species where no ortholog for MGMT was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • oppossum (Monodelphis domestica)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea squirt (Ciona intestinalis)
  • sea squirt (Ciona savignyi)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for MGMT Gene

ENSEMBL:
Gene Tree for MGMT (if available)
TreeFam:
Gene Tree for MGMT (if available)

Paralogs for MGMT Gene

No data available for Paralogs for MGMT Gene

Variants for MGMT Gene

Sequence variations from dbSNP and Humsavar for MGMT Gene

SNP ID Clin Chr 10 pos Sequence Context AA Info Type
rs748231906 Uncertain significance 129,536,279(+) AAACG(C/T)ACCAC reference, synonymous-codon
rs1000003241 -- 129,613,959(+) TACAT(A/G)AAATT intron-variant
rs1000006433 -- 129,490,998(+) TGTTG(C/T)ATTTT intron-variant
rs1000011052 -- 129,732,631(+) ATACA(G/T)GTGCC intron-variant
rs1000025737 -- 129,763,402(+) AAATA(G/T)TGCCT intron-variant

Structural Variations from Database of Genomic Variants (DGV) for MGMT Gene

Variant ID Type Subtype PubMed ID
dgv174e199 CNV deletion 23128226
esv21706 CNV loss 19812545
esv26422 CNV gain 19812545
esv2673495 CNV deletion 23128226
esv2673844 CNV deletion 23128226
esv2743445 CNV deletion 23290073
esv2762887 CNV loss 21179565
esv3347457 CNV insertion 20981092
esv33589 CNV loss 17666407
esv3412267 CNV duplication 20981092
esv3577850 CNV gain 25503493
esv3579230 CNV loss 25503493
esv3624921 CNV loss 21293372
esv3624922 CNV loss 21293372
nsv1043794 CNV loss 25217958
nsv1053385 CNV loss 25217958
nsv1054536 CNV gain 25217958
nsv1069521 CNV deletion 25765185
nsv1122569 CNV deletion 24896259
nsv24217 CNV insertion 16902084
nsv477125 CNV novel sequence insertion 20440878
nsv513300 CNV insertion 21212237
nsv516662 CNV loss 19592680
nsv7617 CNV insertion 18451855

Variation tolerance for MGMT Gene

Residual Variation Intolerance Score: 58% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 6.20; 76.01% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for MGMT Gene

Human Gene Mutation Database (HGMD)
MGMT
SNPedia medical, phenotypic, and genealogical associations of SNPs for
MGMT

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for MGMT Gene

Disorders for MGMT Gene

MalaCards: The human disease database

(36) MalaCards diseases for MGMT Gene - From: Orphanet, DISEASES, Novoseek, and GeneCards

Disorder Aliases PubMed IDs
malignant glioma
primary central nervous system lymphoma
  • pcnsl
glioblastoma
  • glioblastoma multiforme
brain cancer
  • adult brain tumor
spinal cord astrocytoma
  • astrocytoma of spinal cord
- elite association - COSMIC cancer census association via MalaCards
Search MGMT in MalaCards View complete list of genes associated with diseases

Relevant External Links for MGMT

Genetic Association Database (GAD)
MGMT
Human Genome Epidemiology (HuGE) Navigator
MGMT
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
MGMT
genes like me logo Genes that share disorders with MGMT: view

No data available for UniProtKB/Swiss-Prot and Genatlas for MGMT Gene

Publications for MGMT Gene

  1. Polymorphisms of the DNA repair gene MGMT and risk and progression of head and neck cancer. (PMID: 20206583) Zhang Z. … Wei Q. (DNA Repair (Amst.) 2010) 3 22 46 64
  2. Effect of DNA repair host factors on temozolomide or dacarbazine melanoma treatment in Caucasians. (PMID: 19741564) Boeckmann L. … Emmert S. (Pharmacogenet. Genomics 2009) 3 22 46 64
  3. MGMT methylation is associated primarily with the germline C>T SNP (rs16906252) in colorectal cancer and normal colonic mucosa. (PMID: 19734844) Hawkins N.J. … Hitchins M.P. (Mod. Pathol. 2009) 3 22 46 64
  4. Association between MGMT promoter hypermethylation and p53 mutation in glioblastoma. (PMID: 19544111) Shamsara J. … Behravan J. (Cancer Invest. 2009) 3 22 46 64
  5. High incidence of MGMT promoter methylation in primary glioblastomas without correlation with TP53 gene mutations. (PMID: 19100509) Jesien-Lewandowicz E. … Kordek R. (Cancer Genet. Cytogenet. 2009) 3 22 46 64

Products for MGMT Gene

Sources for MGMT Gene

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