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METTL20 Gene

protein-coding   GIFtS: 36
GCID: GC12P031800

Methyltransferase Like 20

(Previous name: chromosome 12 open reading frame 72)
(Previous symbol: C12orf72)
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(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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Aliases
Methyltransferase Like 201 2
C12orf721 2 3 5
Chromosome 12 Open Reading Frame 721
Methyltransferase-Like Protein 202
EC 2.1.1.-3

External Ids:    HGNC: 287391   Entrez Gene: 2540132   Ensembl: ENSG000001391607   OMIM: 6152565   UniProtKB: Q8IXQ93   

Export aliases for METTL20 gene to outside databases


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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GeneCards Summary for METTL20 Gene:
METTL20 (methyltransferase like 20) is a protein-coding gene. GO annotations related to this gene include protein methyltransferase activity.

UniProtKB/Swiss-Prot: MET20_HUMAN, Q8IXQ9
Function: Probable methyltransferase (By similarity)




(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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Regulatory elements:
   Search for regulatory transcription factor binding sites for METTL20
         Other transcription factors

Browse SwitchGear Promoter luciferase reporter plasmids
   Search Chromatin IP Primers for METTL20

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat METTL20


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 12p11.21   Ensembl cytogenetic band:  12p11.21   HGNC cytogenetic band: 12p11.21

METTL20 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
METTL20 gene location

GeneLoc information about chromosome 12         GeneLoc Exon Structure

GeneLoc location for GC12P031800:  view genomic region     (about GC identifiers)

Start:
31,800,094 bp from pter      End:
31,826,048 bp from pter
Size:
25,955 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, Cloud-Clone Corp., eBioscience, and/or antibodies-online,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, Cloud-Clone Corp., eBioscience, and/or antibodies-online, Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, Cloud-Clone Corp, antibodies-online, and/or others.)
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UniProtKB/Swiss-Prot: MET20_HUMAN, Q8IXQ9 (See protein sequence)
Recommended Name: Methyltransferase-like protein 20  
Size: 262 amino acids; 29461 Da
Subunit: Interacts with HSPD1; this protein may possibly be a methylation substrate
Secondary accessions: D3DUW3

Explore the universe of human proteins at neXtProt for METTL20: NX_Q8IXQ9

Explore proteomics data for METTL20 at MOPED


See METTL20 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

REFSEQ proteins (3 alternative transcripts): 
NP_001129335.1  NP_001129336.1  NP_776163.1  

ENSEMBL proteins: 
 ENSP00000396123   ENSP00000379112   ENSP00000441421   ENSP00000350353   ENSP00000445165  
 ENSP00000437442  

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antibodies-online proteins for METTL20 (7 products) 

 
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(According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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1 InterPro protein domain:
 IPR010456 Ribosomal-L11_MeTrfase_PrmA

Graphical View of Domain Structure for InterPro Entry Q8IXQ9

ProtoNet protein and cluster: Q8IXQ9

UniProtKB/Swiss-Prot: MET20_HUMAN, Q8IXQ9
Similarity: Belongs to the methyltransferase superfamily. METTL20 family


Find genes that share domains with METTL20           About GenesLikeMe


(According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, genOway,
transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, Vector BioLabs and/or Addgene, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Flow cytometry from eBioscience, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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Molecular Function:

     UniProtKB/Swiss-Prot Summary: MET20_HUMAN, Q8IXQ9
Function: Probable methyltransferase (By similarity)

     Enzyme Number (IUBMB): EC 2.1.1.-1

     Gene Ontology (GO): 1 molecular function term:    About this table

GO IDQualified GO termEvidencePubMed IDs
GO:0008276protein methyltransferase activity IEA--
     
Find genes that share ontologies with METTL20           About GenesLikeMe


Phenotypes:
     1 GenomeRNAi human phenotype for METTL20:
 Synthetic lethal with Ras 

     1 MGI phenotypic allele for Mettl20 (no phenotypes)

Find genes that share phenotypes with METTL20           About GenesLikeMe

Animal Models:
   genOway: Develop your customized and physiologically relevant rodent model for METTL20

miRNA
Products:
    
miRTarBase miRNAs that target METTL20:
hsa-mir-532-5p (MIRT040981)

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2 qRT-PCR Assays for microRNAs that regulate METTL20:
hsa-miR-2355-3p hsa-miR-3909
SwitchGear 3'UTR luciferase reporter plasmidMETTL20 3' UTR sequence
Inhib. RNA
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(According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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Subcellular locations from UniProtKB/Swiss-Prot
MET20_HUMAN, Q8IXQ9: Cytoplasm. Note=Concentrated in cytoplasmic granular foci

Gene Ontology (GO): 1 cellular component term:    About this table

GO IDQualified GO termEvidencePubMed IDs
GO:0005737cytoplasm IEA--

Find genes that share ontologies with METTL20           About GenesLikeMe


(SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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    Custom Pathway & Disease-focused RT2 Profiler PCR Arrays for METTL20
Interactions:

    Search GeneGlobe Interaction Network for METTL20

STRING Interaction Network Preview (showing 4 interactants - click image to see more details)

4 Interacting proteins for METTL20 (ENSP000003503534) via UniProtKB, MINT, STRING, and/or I2D
InteractantInteraction Details
GeneCardExternal ID(s)
CNOT4ENSP000003546734STRING: ENSP00000354673
TPT1ENSP000003683504STRING: ENSP00000368350
HCCSENSP000003265794STRING: ENSP00000326579
--ENSP000004115504STRING: ENSP00000411550
About this table

Gene Ontology (GO): 1 biological process term:    About this table

GO IDQualified GO termEvidencePubMed IDs
GO:0006479protein methylation ----

Find genes that share ontologies with METTL20           About GenesLikeMe



(Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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Browse Small Molecules at EMD Millipore
   Browse drugs & compounds from Enzo Life Sciences
  Browse compounds at ApexBio 

Browse Tocris compounds for METTL20 (MET20)



(Secondary structures according to fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, and/or Addgene, Primers from OriGene, and/or QIAGEN, Flow cytometry from eBioscience )
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REFSEQ mRNAs for METTL20 gene (3 alternative transcripts): 
NM_001135863.1  NM_001135864.1  NM_173802.3  

6 Ensembl transcripts including schematic representations, and UCSC links where relevant:
ENST00000412352 ENST00000395763(uc001rkl.3) ENST00000538463 ENST00000357721(uc009zjr.3 uc001rkm.3)
ENST00000539633 ENST00000538391
miRNA
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2 qRT-PCR Assays for microRNAs that regulate METTL20:
hsa-miR-2355-3p hsa-miR-3909
SwitchGear 3'UTR luciferase reporter plasmidMETTL20 3' UTR sequence
Inhib. RNA
Products:
     
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Predesigned siRNA for gene silencing in human, mouse, rat METTL20
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  QuantiFast Probe-based Assays in human, mouse, rat METTL20

Selected AceView cDNA sequences (see all 35):

CR607465 BM129337 CD365452 BC039535 NM_173802 BM129806 AA911463 CK819600 
BM129614 AA810345 BX444540 BC039107 BX440980 AI910578 AI302347 AW272460 
BQ012226 AL832339 BM559730 AI149303 BC039417 F20667 BX098492 BM460012 

GeneLoc Exon Structure


(RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
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METTL20 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS <intensity>2/3
CGAP TAG: --
METTL20 Expression
About this image


METTL20 expression in embryonic tissues and stem cells    About this table
Data from LifeMap, the Embryonic Development and Stem Cells Database
 selected tissues (see all 2) fully expand
 
 Testis (Reproductive System)
         Spermatid Seminiferous Tubules
 
 Gonad
         Spermatid Seminiferous Tubules
METTL20 Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

METTL20 Protein Expression
    Custom PCR Arrays for METTL20
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In Situ
Assay Products:
 

 
Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for METTL20

(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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This gene was present in the common ancestor of animals.

Orthologs for METTL20 gene from Selected species (see all 14)    About this table
Organism Taxonomic
classification
Gene Description Human
Similarity
Orthology
Type
Details
mouse
(Mus musculus)
Mammalia Mettl201 , 5 RIKEN cDNA 4833442J19 gene5
methyltransferase like 201
82.22(n)1
78.82(a)1
  6 (78.80 cM)5
3202041  NM_001252096.11  NP_001239025.11 
 1491398495 
chicken
(Gallus gallus)
Aves METTL201 methyltransferase like 20 72.37(n)
73.68(a)
  418138  XM_416369.3  XP_416369.1 
lizard
(Anolis carolinensis)
Reptilia METTL206
methyltransferase like 20
55(a)
1 ↔ 1
5(10653201-10664388)
tropical clawed frog
(Xenopus tropicalis)
Amphibia LOC1004865451 methyltransferase-like protein 20-like 61.68(n)
61.21(a)
  100486545  XM_002941617.2  XP_002941663.1 
zebrafish
(Danio rerio)
Actinopterygii mettl201 methyltransferase like 20 61.27(n)
59.72(a)
  100005455  NM_001161495.1  NP_001154967.1 
mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP0076521 AgaP_AGAP007652 48.67(n)
39.91(a)
  1269575  XM_308218.4  XP_308218.4 
worm
(Caenorhabditis elegans)
Secernentea C37A2.61 C37A2.6 52.07(n)
44.28(a)
  183284  NM_059542.2  NP_491943.2 


ENSEMBL Gene Tree for METTL20 (if available)
TreeFam Gene Tree for METTL20 (if available)

(Paralogs according to 1HomoloGene,
2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
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(SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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Selected SNPs for METTL20 (see all 593)    About this table                                 

Genomic DataTranscription Related DataAllele Frequencies
SNP IDValidClinical
significance
Chr 12 posSequence#AA
Chg
TypeMore#Allele
freq
PopTotal
sample
More
----------
rs73001901,2
C,F--31798182(+) tttttA/Gataga 1 -- us2k110Minor allele frequency- G:0.33NA WA CSA EA 372
rs1817315161,2
C--31798215(+) CAGGCC/TGGTCT 1 -- us2k10--------
rs114407941,2
C--31798301(+) TTTTT-/T/T   
   TTTTT
TTTTT
2 -- us2k1 cds11NA 2
rs169188431,2
C,F,H--31798405(+) AGGAAG/CTCTTT 1 -- us2k110Minor allele frequency- C:0.12NA NS EA 862
rs1869031171,2
--31798485(+) TTTGGC/TCTCAG 1 -- us2k10--------
rs769187101,2
F--31798591(+) TAGAGT/CTGCAC 1 -- us2k11Minor allele frequency- C:0.02EA 120
rs765154651,2
C,F--31798691(+) AGCAGG/AGGAAG 1 -- us2k11Minor allele frequency- A:0.07EA 120
rs1897039521,2
--31798730(+) TTACAA/TGGATA 1 -- us2k10--------
rs1814770941,2
--31798737(+) GATAAA/CAATTT 1 -- us2k10--------
rs733018901,2
C--31798794(+) TGATGT/CTTTTC 1 -- us2k12Minor allele frequency- C:0.07WA 120

HapMap Linkage Disequilibrium report for METTL20 (31800094 - 31826048 bp)

Structural Variations
     Database of Genomic Variants (DGV) 6 variations for METTL20:    About this table    
Variant IDTypeSubtypePubMed ID
esv1351884CNV Insertion17803354
esv2495218CNV Insertion19546169
nsv662CNV Insertion18451855
nsv522379CNV Loss19592680
nsv832365CNV Loss17160897
nsv898966CNV Gain21882294

Site Specific Mutation Identification with PCR Assays
SeqTarget long-range PCR primers for resequencing METTL20
DNA2.0 Custom Variant and Variant Library Synthesis for METTL20

(in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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OMIM gene information: 615256    OMIM disorders: --


Find genes that share disorders with METTL20           About GenesLikeMe


Export disorders for METTL20 gene to outside databases

(in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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PubMed articles for METTL20 gene integrated from 10 sources:
(articles sorted by number of sources associating them with METTL20)
    Utopia: connect your pdf to the dynamic
world of online information

  1. A newly uncovered group of distantly related lysine methyltransferases preferentially interact with molecular chaperones to regulate their activity. (PubMed id 23349634)1, 2 Cloutier P.... Coulombe B. (PLoS Genet. 2013)
  2. hORFeome v3.1: a resource of human open reading frames representing over 10,000 human genes. (PubMed id 17207965)1 Lamesch P.... Vidal M. (Genomics 2007)
  3. Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. (PubMed id 16344560)1 Kimura K.... Sugano S. (Genome Res. 2006)
  4. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)2 Gerhard D.S.... Malek J. (Genome Res. 2004)
  5. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)2 Ota T.... Sugano S. (Nat. Genet. 2004)
  6. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1 Strausberg R.L....Marra M.A. (Proc. Natl. Acad. Sci. U.S.A. 2002)

(in PubMed, OMIM, and NCBI Bookshelf)
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 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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Entrez Gene: 254013 HGNC: 28739 AceView: MGC50559 Ensembl:ENSG00000139160 euGenes: HUgn254013
ECgene: METTL20 H-InvDB: METTL20

(According to HUGE)
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  --

(According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
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NameDescription
PharmGKB entry for METTL20 Pharmacogenomics, SNPs, Pathways

(Patent information from GeneIP,
Licensable technologies from WIS Yeda, Salk, Tufts,
IP news from LifeMap Sciences, Inc.)
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Patent Information for METTL20 gene:
Search GeneIP for patents involving METTL20

GeneCards and IP:
Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, eBioscience, antibodies-online, and/or others, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Addgene, Cell lines from GenScript, and ESI BIO, Flow cytometery from eBioscience, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
Advanced Cell Diagnostics, Animal models from genOway)
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 DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat METTL20 Predesigned siRNA for gene silencing in human, mouse, rat METTL20
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 GenScript Custom Purified and Recombinant Proteins Services for METTL20 GenScript cDNA clones with any tag delivered in your preferred vector for METTL20
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 Search www.enzolifesciences.com for proteins, assays, substrates, inhibitors & antibodies

 Novus Tissue Slides
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 Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat METTL20
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 antibodies-online proteins for METTL20 (7 products)
       
GeneCards Homepage - Last full update: 7 May 2014 - Incrementals: 9 May 2014 , 2 Jun 2014 , 26 Jun 2014 , 30 Jun 2014 , 21 Aug 2014 , 8 Sep 2014 , 7 Oct 2014

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