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Aliases for METTL14 Gene

Aliases for METTL14 Gene

  • Methyltransferase Like 14 2 3 5
  • Methyltransferase-Like Protein 14 3 4
  • HMETTL14 3 4
  • N6-Adenosine-Methyltransferase Subunit METTL14 3
  • EC 2.1.1.62 4
  • KIAA1627 4

External Ids for METTL14 Gene

Previous GeneCards Identifiers for METTL14 Gene

  • GC04P119825
  • GC04P119607
  • GC04P115328
  • GC04P119621
  • GC04P119636

Summaries for METTL14 Gene

GeneCards Summary for METTL14 Gene

METTL14 (Methyltransferase Like 14) is a Protein Coding gene. Among its related pathways are Gene Expression and mRNA Splicing - Major Pathway. GO annotations related to this gene include RNA binding and mRNA (2-O-methyladenosine-N6-)-methyltransferase activity.

UniProtKB/Swiss-Prot for METTL14 Gene

  • The METTL3-METTL14 heterodimer forms a N6-methyltransferase complex that methylates adenosine residues of some mRNAs and regulates the circadian clock and differentiation of embryonic stem cells (PubMed:24316715, PubMed:24407421, PubMed:25719671, PubMed:27373337, PubMed:27281194). In the heterodimer formed with METTL3, METTL14 constitutes the RNA-binding scaffold that recognizes the substrate rather than the catalytic core (PubMed:27373337, PubMed:27281194). N6-methyladenosine (m6A), which takes place at the 5-[AG]GAC-3 consensus sites of some mRNAs, plays a role in the efficiency of mRNA splicing, processing and mRNA stability (PubMed:24316715, PubMed:24407421, PubMed:25719671). M6A regulates the length of the circadian clock: acts as an early pace-setter in the circadian loop. M6A also acts as a regulator of mRNA stability: in embryonic stem cells (ESCs), m6A methylation of mRNAs encoding key naive pluripotency-promoting transcripts results in transcript destabilization (By similarity).

No data available for Entrez Gene Summary , CIViC summary , Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for METTL14 Gene

Genomics for METTL14 Gene

Regulatory Elements for METTL14 Gene

Enhancers for METTL14 Gene
GeneHancer Identifier Enhancer Score Enhancer Sources Gene-Enhancer Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Gene Targets for Enhancer
GH04G118765 1.3 Ensembl ENCODE dbSUPER 8.8 +80.4 80355 0.7 TAL1 JUN FEZF1 FOSL1 TCF12 GATA2 ZNF366 NCOR1 SCRT2 FOSL2 SEC24D METTL14 CEP170P1 GC04P118778
GH04G118774 1.1 Ensembl ENCODE dbSUPER 10.1 +89.8 89784 1.5 ZNF316 MAFG FOS HNF4A NFE2 EMSY PRDM1 MAFK METTL14 LOC729218 GC04P118778
GH04G118683 1.3 Ensembl ENCODE 8.5 -0.2 -234 3.5 HDGF PKNOX1 CREB3L1 WRNIP1 ARID4B SIN3A DMAP1 YY1 ZNF143 SP3 METTL14 CEP170P1 SNHG8 LOC101929741 GC04P118788
GH04G118833 1.3 ENCODE dbSUPER 6.3 +150.0 150038 4.0 HDGF PKNOX1 CREB3L1 ARNT ARID4B SIN3A DMAP1 ZNF2 YY1 ZNF143 METTL14 SNHG8 SEC24D GC04P118778
GH04G118590 1.1 ENCODE 7.4 -93.6 -93624 1.7 CREB3L1 WRNIP1 ARID4B SIN3A DMAP1 ZNF2 YY1 ZNF143 ZNF207 ZNF263 LOC729218 METTL14 SNHG8 GC04M118372
- Elite enhancer and/or Elite enhancer-gene association Download GeneHancer data dump

Enhancers around METTL14 on UCSC Golden Path with GeneCards custom track

Promoters for METTL14 Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters
ENSR00000172376 337 1800 HDGF PKNOX1 CREB3L1 WRNIP1 ARID4B SIN3A DMAP1 YY1 ZNF143 SP3

Genomic Location for METTL14 Gene

Chromosome:
4
Start:
118,685,364 bp from pter
End:
118,715,433 bp from pter
Size:
30,070 bases
Orientation:
Plus strand

Genomic View for METTL14 Gene

Genes around METTL14 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
METTL14 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for METTL14 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for METTL14 Gene

Proteins for METTL14 Gene

  • Protein details for METTL14 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q9HCE5-MET14_HUMAN
    Recommended name:
    N6-adenosine-methyltransferase subunit METTL14
    Protein Accession:
    Q9HCE5
    Secondary Accessions:
    • A6NIG1
    • Q969V2

    Protein attributes for METTL14 Gene

    Size:
    456 amino acids
    Molecular mass:
    52150 Da
    Quaternary structure:
    • Heterodimer; heterodimerizes with METTL3 to form an antiparallel heterodimer that constitutes an active methyltransferase (PubMed:27373337, PubMed:27281194). Component of the WMM complex, a N6-methyltransferase complex composed of WTAP, METTL3 and METTL14.
    SequenceCaution:
    • Sequence=BAB13453.1; Type=Erroneous initiation; Evidence={ECO:0000305};

    Three dimensional structures from OCA and Proteopedia for METTL14 Gene

neXtProt entry for METTL14 Gene

Post-translational modifications for METTL14 Gene

  • Phosphorylation at Ser-399 is important for interaction with METTL3: phosphorylated Ser-399 forms a salt bridge with Arg-471 of METTL3.
  • Ubiquitination at posLast=148148
  • Modification sites at PhosphoSitePlus

Other Protein References for METTL14 Gene

No data available for DME Specific Peptides for METTL14 Gene

Domains & Families for METTL14 Gene

Protein Domains for METTL14 Gene

InterPro:
Blocks:
ProtoNet:

Suggested Antigen Peptide Sequences for METTL14 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

Q9HCE5

UniProtKB/Swiss-Prot:

MET14_HUMAN :
  • Belongs to the MT-A70-like family.
Family:
  • Belongs to the MT-A70-like family.
genes like me logo Genes that share domains with METTL14: view

Function for METTL14 Gene

Molecular function for METTL14 Gene

UniProtKB/Swiss-Prot CatalyticActivity:
S-adenosyl-L-methionine + m(7)G(5)pppAm = S-adenosyl-L-homocysteine + m(7)G(5)pppm(6)Am.
UniProtKB/Swiss-Prot Function:
The METTL3-METTL14 heterodimer forms a N6-methyltransferase complex that methylates adenosine residues of some mRNAs and regulates the circadian clock and differentiation of embryonic stem cells (PubMed:24316715, PubMed:24407421, PubMed:25719671, PubMed:27373337, PubMed:27281194). In the heterodimer formed with METTL3, METTL14 constitutes the RNA-binding scaffold that recognizes the substrate rather than the catalytic core (PubMed:27373337, PubMed:27281194). N6-methyladenosine (m6A), which takes place at the 5-[AG]GAC-3 consensus sites of some mRNAs, plays a role in the efficiency of mRNA splicing, processing and mRNA stability (PubMed:24316715, PubMed:24407421, PubMed:25719671). M6A regulates the length of the circadian clock: acts as an early pace-setter in the circadian loop. M6A also acts as a regulator of mRNA stability: in embryonic stem cells (ESCs), m6A methylation of mRNAs encoding key naive pluripotency-promoting transcripts results in transcript destabilization (By similarity).

Enzyme Numbers (IUBMB) for METTL14 Gene

Gene Ontology (GO) - Molecular Function for METTL14 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003723 RNA binding IEA --
GO:0003729 mRNA binding IDA 27281194
GO:0005515 protein binding IPI 27281194
GO:0008168 methyltransferase activity IEA --
GO:0016422 contributes_to mRNA (2-O-methyladenosine-N6-)-methyltransferase activity IEA,IDA 27281194
genes like me logo Genes that share ontologies with METTL14: view
genes like me logo Genes that share phenotypes with METTL14: view

Animal Model Products

CRISPR Products

miRNA for METTL14 Gene

miRTarBase miRNAs that target METTL14

No data available for Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for METTL14 Gene

Localization for METTL14 Gene

Subcellular locations from UniProtKB/Swiss-Prot for METTL14 Gene

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for METTL14 gene
Compartment Confidence
nucleus 5

Gene Ontology (GO) - Cellular Components for METTL14 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IEA,IDA 24316715
GO:0005654 nucleoplasm TAS --
GO:0036396 MIS complex IDA 24316715
genes like me logo Genes that share ontologies with METTL14: view

Pathways & Interactions for METTL14 Gene

genes like me logo Genes that share pathways with METTL14: view

Pathways by source for METTL14 Gene

Gene Ontology (GO) - Biological Process for METTL14 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000398 mRNA splicing, via spliceosome IMP 25719671
GO:0006139 nucleobase-containing compound metabolic process IEA --
GO:0010467 gene expression TAS --
GO:0016070 RNA metabolic process TAS --
GO:0019827 stem cell population maintenance ISS,IEA --
genes like me logo Genes that share ontologies with METTL14: view

No data available for SIGNOR curated interactions for METTL14 Gene

Transcripts for METTL14 Gene

Unigene Clusters for METTL14 Gene

Methyltransferase like 14:
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for METTL14 Gene

ExUns: 1a · 1b ^ 2 ^ 3 ^ 4a · 4b ^ 5a · 5b ^ 6 ^ 7 ^ 8 ^ 9 ^ 10 ^ 11 ^ 12 ^ 13 ^ 14a · 14b
SP1: - - -
SP2:
SP3: - -
SP4:

Relevant External Links for METTL14 Gene

GeneLoc Exon Structure for
METTL14
ECgene alternative splicing isoforms for
METTL14

Expression for METTL14 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and CGAP SAGE for METTL14 Gene

Protein differential expression in normal tissues from HIPED for METTL14 Gene

This gene is overexpressed in Platelet (41.2) and Adrenal (6.3).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MaxQB, and MOPED for METTL14 Gene



Protein tissue co-expression partners for METTL14 Gene

- Elite partner

NURSA nuclear receptor signaling pathways regulating expression of METTL14 Gene:

METTL14

SOURCE GeneReport for Unigene cluster for METTL14 Gene:

Hs.657806

Evidence on tissue expression from TISSUES for METTL14 Gene

  • Liver(4.2)
  • Lung(4.2)
  • Muscle(4.2)
  • Nervous system(4.2)
genes like me logo Genes that share expression patterns with METTL14: view

Primer Products

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , mRNA differential expression in normal tissues , mRNA Expression by UniProt/SwissProt and Phenotype-based relationships between genes and organs from Gene ORGANizer for METTL14 Gene

Orthologs for METTL14 Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for METTL14 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia METTL14 34 35
  • 99.85 (n)
dog
(Canis familiaris)
Mammalia METTL14 34 35
  • 95.88 (n)
cow
(Bos Taurus)
Mammalia METTL14 34 35
  • 95.39 (n)
platypus
(Ornithorhynchus anatinus)
Mammalia METTL14 35
  • 95 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Mettl14 34 16 35
  • 91.23 (n)
rat
(Rattus norvegicus)
Mammalia Mettl14 34
  • 89.07 (n)
oppossum
(Monodelphis domestica)
Mammalia METTL14 35
  • 83 (a)
OneToOne
chicken
(Gallus gallus)
Aves METTL14 34 35
  • 83.92 (n)
lizard
(Anolis carolinensis)
Reptilia METTL14 35
  • 93 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia mettl14 34
  • 76.94 (n)
Str.3176 34
African clawed frog
(Xenopus laevis)
Amphibia Xl.7634 34
zebrafish
(Danio rerio)
Actinopterygii mettl14 34 35
  • 72.74 (n)
fruit fly
(Drosophila melanogaster)
Insecta CG7818 34 35
  • 59.91 (n)
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP008111 34
  • 57.16 (n)
K. lactis yeast
(Kluyveromyces lactis)
Saccharomycetes KLLA0C00693g 34
  • 49.44 (n)
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes KAR4 34 35
  • 46.34 (n)
A. gosspyii yeast
(Ashbya gossypii)
Saccharomycetes AGOS_AFR736C 34
  • 43.9 (n)
soybean
(Glycine max)
eudicotyledons Gma.10486 34
barley
(Hordeum vulgare)
Liliopsida Hv.5256 34
rice
(Oryza sativa)
Liliopsida Os.22508 34
sea squirt
(Ciona savignyi)
Ascidiacea CSA.7357 35
  • 55 (a)
OneToOne
sea squirt
(Ciona intestinalis)
Ascidiacea Cin.7408 34
Species where no ortholog for METTL14 was found in the sources mined by GeneCards:
  • Actinobacteria (Mycobacterium tuberculosis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for METTL14 Gene

ENSEMBL:
Gene Tree for METTL14 (if available)
TreeFam:
Gene Tree for METTL14 (if available)

Paralogs for METTL14 Gene

No data available for Paralogs for METTL14 Gene

Variants for METTL14 Gene

Sequence variations from dbSNP and Humsavar for METTL14 Gene

SNP ID Clin Chr 04 pos Sequence Context AA Info Type
rs1000171053 -- 118,686,395(+) TCGAG(A/G)CTGTA intron-variant, upstream-variant-2KB
rs10002843 -- 118,687,821(+) AAGGT(A/G)TTTTT intron-variant
rs1000351122 -- 118,684,947(+) TATGA(C/T)TCCCA intron-variant, upstream-variant-2KB
rs1000354689 -- 118,709,549(+) GTAAC(A/G)TGGTA intron-variant
rs1000385956 -- 118,698,908(+) GGGAT(C/T)GTGTC intron-variant

Structural Variations from Database of Genomic Variants (DGV) for METTL14 Gene

Variant ID Type Subtype PubMed ID
esv2759279 CNV gain 17122850
nsv519017 CNV loss 19592680

Variation tolerance for METTL14 Gene

Residual Variation Intolerance Score: 32.6% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 0.29; 6.38% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for METTL14 Gene

Human Gene Mutation Database (HGMD)
METTL14
SNPedia medical, phenotypic, and genealogical associations of SNPs for
METTL14

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for METTL14 Gene

Disorders for METTL14 Gene

Relevant External Links for METTL14

Genetic Association Database (GAD)
METTL14
Human Genome Epidemiology (HuGE) Navigator
METTL14
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
METTL14

No disorders were found for METTL14 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for METTL14 Gene

Publications for METTL14 Gene

  1. A METTL3-METTL14 complex mediates mammalian nuclear RNA N-adenosine methylation. (PMID: 24316715) Liu J. … He C. (Nat. Chem. Biol. 2014) 2 3 4 64
  2. Prediction of the coding sequences of unidentified human genes. XVIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. (PMID: 10997877) Nagase T. … Ohara O. (DNA Res. 2000) 2 3 4 64
  3. Structural basis of N(6)-adenosine methylation by the METTL3-METTL14 complex. (PMID: 27281194) Wang X. … Yin P. (Nature 2016) 3 4 64
  4. Structural basis for cooperative function of Mettl3 and Mettl14 methyltransferases. (PMID: 27373337) Wang P. … Nam Y. (Mol. Cell 2016) 3 4 64
  5. N(6)-methyladenosine-dependent RNA structural switches regulate RNA-protein interactions. (PMID: 25719671) Liu N. … Pan T. (Nature 2015) 3 4 64

Products for METTL14 Gene

Sources for METTL14 Gene

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