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METAP2 Gene

protein-coding   GIFtS: 69
GCID: GC12P095800

Methionyl Aminopeptidase 2

  See METAP2-related disease
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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Aliases
Methionyl Aminopeptidase 21 2     p67eIF22 3
p672 3 5     MAP22
Peptidase M1 3     EIF-2-Associated P67 Homolog2
Initiation Factor 2-Associated 67 KDa Glycoprotein2 3     Methionine Aminopeptidase 22
MetAP 22 3     Peptidase M 22
MNPEP2 3     EC 3.4.11.183
MAP 22 3     P67EIF23

External Ids:    HGNC: 166721   Entrez Gene: 109882   Ensembl: ENSG000001111427   OMIM: 6018705   UniProtKB: P505793   

Export aliases for METAP2 gene to outside databases

Previous GC identifers: GC12M095311 GC12P096231 GC12P094370 GC12P092936


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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Entrez Gene summary for METAP2 Gene:
This gene is a member of the methionyl aminopeptidase family and encodes a protein that binds 2 cobalt or
manganese ions. This protein functions both by protecting the alpha subunit of eukaryotic initiation factor 2
from inhibitory phosphorylation and by removing the amino-terminal methionine residue from nascent protein.
Increased expression of this gene is associated with various forms of cancer and the anti-cancer drugs fumagillin
and ovalicin inhibit the protein by irreversibly binding to its active site. A pseudogene of this gene is located
on chromosome 2. (provided by RefSeq, Jul 2008)

GeneCards Summary for METAP2 Gene:
METAP2 (methionyl aminopeptidase 2) is a protein-coding gene. Diseases associated with METAP2 include microsporidiosis. GO annotations related to this gene include metalloexopeptidase activity and aminopeptidase activity. An important paralog of this gene is ENSG00000257411.

UniProtKB/Swiss-Prot: MAP2_HUMAN, P50579
Function: Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is
often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro,
Ser, Thr, or Val). The catalytic activity of human METAP2 toward Met-Val peptides is consistently two orders of
magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing
N-terminal Met-Val and Met-Thr sequences in vivo
Function: Protects eukaryotic initiation factor EIF2S1 from translation-inhibiting phosphorylation by inhibitory
kinases such as EIF2AK2/PKR and EIF2AK1/HCR. Plays a critical role in the regulation of protein synthesis

Gene Wiki entry for METAP2 Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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RefSeq DNA sequence at NCBI GenBank:
NC_000012.11  NT_029419.13  NC_018923.2  
Regulatory elements:
   Regulatory transcription factor binding sites in the METAP2 gene promoter:
         AML1a   AP-1   ATF-2   HSF1 (long)   Max   NRF-2   HSF1short   c-Jun   c-Myc   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidMETAP2 promoter sequence
   Search Chromatin IP Primers for METAP2

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat METAP2


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 12q22   Ensembl cytogenetic band:  12q22   HGNC cytogenetic band: 12q22

METAP2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
METAP2 gene location

GeneLoc information about chromosome 12         GeneLoc Exon Structure

GeneLoc location for GC12P095800:  view genomic region     (about GC identifiers)

Start:
95,867,296 bp from pter      End:
95,909,615 bp from pter
Size:
42,320 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, Cloud-Clone Corp., and/or eBioscience,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, Cloud-Clone Corp., and/or eBioscience, Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, Cloud-Clone Corp, and/or others.)
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UniProtKB/Swiss-Prot: MAP2_HUMAN, P50579 (See protein sequence)
Recommended Name: Methionine aminopeptidase 2  
Size: 478 amino acids; 52892 Da
Cofactor: Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site.
The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese
or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe(2+)-metalloproteases
under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the
histidine-containing high-affinity site (By similarity). Also manganese has been proposed to be the physiological
cofactor for human METAP2
Subunit: Interacts strongly with the eIF-2 gamma-subunit EIF2S3 (By similarity). Binds EIF2S1 at low magnesium
concentrations
Selected PDB 3D structures from and Proteopedia for METAP2 (see all 18):
1B59 (3D)        1B6A (3D)        1BN5 (3D)        1BOA (3D)        1KQ0 (3D)        1KQ9 (3D)    
Secondary accessions: B2RDI8

Explore the universe of human proteins at neXtProt for METAP2: NX_P50579

Explore proteomics data for METAP2 at MOPED

Post-translational modifications: 

  • Contains approximately 12 O-linked N-acetylglucosamine (GlcNAc) residues. O-glycosylation is required for EIF2S1
    binding1
  • Ubiquitination2 at Lys289, Lys427
  • Glycosylation2 at Ser60, Ser63
  • Modification sites at PhosphoSitePlus

  • See METAP2 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins: NP_006829.1  
    ENSEMBL proteins: 
     ENSP00000446052   ENSP00000325312   ENSP00000448169   ENSP00000261220   ENSP00000448370  
     ENSP00000449004   ENSP00000448614   ENSP00000450063   ENSP00000447661  
    Reactome Protein details: P50579

    METAP2 Human Recombinant Protein Products:

    Browse Purified and Recombinant Proteins at EMD Millipore
    R&D Systems Recombinant & Natural Proteins for METAP2 (Methionine Aminopeptidase 2/METAP2)
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Browse OriGene full length recombinant human proteins expressed in human HEK293 cells
    OriGene Protein Over-expression Lysate for METAP2
    OriGene Custom MassSpec
    OriGene Custom Protein Services for METAP2
    GenScript Custom Purified and Recombinant Proteins Services for METAP2
    Sino Biological Recombinant Protein for METAP2
    Sino Biological Cell Lysate for METAP2
    Browse ProSpec Recombinant Proteins
    Cloud-Clone Corp. Proteins for METAP2

     
    Search eBioscience for Proteins for METAP2 

    METAP2 Antibody Products:

    Browse EMD Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    R&D Systems Antibodies for METAP2 (Methionine Aminopeptidase 2/METAP2)
    Cell Signaling Technology (CST) Antibodies for METAP2 
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    Abcam antibodies for METAP2
    Cloud-Clone Corp. Antibodies for METAP2
    ThermoFisher Antibodies for METAP2
    LSBio Antibodies in human, mouse, rat for METAP2

    METAP2 Assay Products:

    Browse Kits and Assays available from EMD Millipore
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    Browse Enzo Life Sciences for kits & assays
    Cloud-Clone Corp. ELISAs for METAP2
    Cloud-Clone Corp. CLIAs for METAP2
    Search eBioscience for ELISAs for METAP2 


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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    IUPHAR Guide to PHARMACOLOGY protein family classification: Methionyl aminopeptidase 2
    Aminopeptidases

    5 InterPro protein domains:
     IPR000994 Pept_M24_structural-domain
     IPR002468 Pept_M24A_MAP2
     IPR018349 Pept_M24A_MAP2_BS
     IPR001714 Pept_M24_MAP
     IPR011991 WHTH_DNA-bd_dom

    Graphical View of Domain Structure for InterPro Entry P50579

    ProtoNet protein and cluster: P50579

    1 Blocks protein domain: IPB001714 Methionine aminopeptidase-1 signature

    UniProtKB/Swiss-Prot: MAP2_HUMAN, P50579
    Similarity: Belongs to the peptidase M24A family. Methionine aminopeptidase eukaryotic type 2 subfamily


    Find genes that share domains with METAP2           About GenesLikeMe


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, Vector BioLabs and/or Addgene, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Flow cytometry from eBioscience, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Molecular Function:

         UniProtKB/Swiss-Prot Summary: MAP2_HUMAN, P50579
    Function: Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is
    often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro,
    Ser, Thr, or Val). The catalytic activity of human METAP2 toward Met-Val peptides is consistently two orders of
    magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing
    N-terminal Met-Val and Met-Thr sequences in vivo
    Function: Protects eukaryotic initiation factor EIF2S1 from translation-inhibiting phosphorylation by inhibitory
    kinases such as EIF2AK2/PKR and EIF2AK1/HCR. Plays a critical role in the regulation of protein synthesis
    Catalytic activity: Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides

         Enzyme Number (IUBMB): EC 3.4.11.181

         Gene Ontology (GO): Selected molecular function terms (see all 6):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0004177aminopeptidase activity IDA8858118
    GO:0005515protein binding ----
    GO:0008235metalloexopeptidase activity IDA8858118
    GO:0044822poly(A) RNA binding IDA--
    GO:0046872metal ion binding IEA--
         
    Find genes that share ontologies with METAP2           About GenesLikeMe


    Phenotypes:
         2 GenomeRNAi human phenotypes for METAP2:
     Increased cell number in G1, s  Synthetic lethal with Ras 

         5 MGI mutant phenotypes (inferred from 2 alleles(MGI details for Metap2):
     cardiovascular system  cellular  embryogenesis  growth/size/body  mortality/aging 

    Find genes that share phenotypes with METAP2           About GenesLikeMe

    Animal Models:
         MGI mouse knock-out Metap2tm1.2Ccr for METAP2

       inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for METAP2
       inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for METAP2

       genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for METAP2
       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for METAP2

    miRNA
    Products:
        
    miRTarBase miRNAs that target METAP2:
    hsa-let-7c-5p (MIRT051737), hsa-mir-124-3p (MIRT022163), hsa-mir-16-5p (MIRT031408), hsa-mir-24-3p (MIRT030371), hsa-mir-155-5p (MIRT020542)

    Block miRNA regulation of human, mouse, rat METAP2 using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate METAP2 (see all 42):
    hsa-miR-323-3p hsa-miR-29a hsa-miR-30d hsa-miR-548l hsa-miR-124 hsa-miR-30a hsa-miR-4330 hsa-miR-506
    SwitchGear 3'UTR luciferase reporter plasmidMETAP2 3' UTR sequence
    Inhib. RNA
    Products:
        
    OriGene RNAi products in human, mouse, rat for METAP2
    Predesigned siRNA for gene silencing in human, mouse, rat METAP2

    Gene Editing
    Products:
    DNA2.0 Custom Protein Engineering Service for METAP2

    Clone
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    OriGene clones in human, mouse for METAP2 (see all 7)
    OriGene ORF clones in mouse, rat for METAP2
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector: METAP2 (NM_006838)
    Sino Biological Human cDNA Clone for METAP2
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for METAP2
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat METAP2

    Cell Line
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    Browse ESI BIO Cell Lines and PureStem Progenitors for METAP2 
    In Situ Assay
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    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for METAP2


    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Subcellular locations from UniProtKB/Swiss-Prot
    MAP2_HUMAN, P50579: Cytoplasm. Note=About 30% of expressed METAP2 associates with polysomes
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    cytosol2
    nucleus2
    chloroplast1
    mitochondrion1

    Gene Ontology (GO): 3 cellular component terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005737cytoplasm IDA--
    GO:0005829cytosol TAS--
    GO:0005886plasma membrane IDA--

    Find genes that share ontologies with METAP2           About GenesLikeMe


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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    SuperPaths for METAP2 About    
    See pathways by source

    SuperPathContained pathways About
    1The phototransduction cascade
    The phototransduction cascade0.51
    Inactivation, recovery and regulation of the phototransduction cascade0.49
    2Diseases associated with visual transduction
    Visual phototransduction0.44
    Diseases associated with visual transduction0.43
    3Signaling by GPCR
    Signal Transduction0.58
    4Translational Control
    Translational Control
    5Disease
    Disease


    Find genes that share SuperPaths with METAP2           About GenesLikeMe

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways

    1 Downloadable PowerPoint Slide of GeneGlobe Pathway Central Maps for METAP2
        JNK Pathway

    1 Cell Signaling Technology (CST) Pathway for METAP2
        Translational Control


    1 Reactome Pathway for METAP2
        Inactivation, recovery and regulation of the phototransduction cascade


        Pathway & Disease-focused RT2 Profiler PCR Arrays including METAP2: 
              Tumor Metastasis in human mouse rat
              Molecular Toxicology PathwayFinder 384HT in human mouse rat

    Interactions:

        GeneGlobe Interaction Network for METAP2

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    Selected Interacting proteins for METAP2 (P505792, 3 ENSP000003253124) via UniProtKB, MINT, STRING, and/or I2D (see all 133)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    CALM1P621583, ENSP000003494674I2D: score=1 STRING: ENSP00000349467
    CALM2P621583I2D: score=1 
    CALM3P621583I2D: score=1 
    EIF2AK2P195253, ENSP000002330574I2D: score=3 STRING: ENSP00000233057
    S100A4P264473, ENSP000003462944I2D: score=3 STRING: ENSP00000346294
    About this table

    Gene Ontology (GO): Selected biological process terms (see all 8):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006508proteolysis ----
    GO:0007603phototransduction, visible light TAS--
    GO:0016056rhodopsin mediated signaling pathway TAS--
    GO:0016485protein processing IDA8858118
    GO:0018206peptidyl-methionine modification IDA8858118

    Find genes that share ontologies with METAP2           About GenesLikeMe



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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    Browse Small Molecules at EMD Millipore
       Enzo Life Sciences drugs & compounds for METAP2
    4 ApexBio Compounds for METAP2     About this table
    CompoundAction CAS #
    BestatinSpecific inhibitor of aminopeptidase B and leucine aminopeptidase[58970-76-6]
    Fumagillininhibits methionine aminopeptidase-2[23110-15-8]
    SC 57461APotent and selective inhibitor of LTA4 hydrolase[423169-68-0]
    Tosedostat (CHR2797)aminopeptidase inhibitor for LAP, PuSA and Aminopeptidase N[238750-77-1]

    Compounds for METAP2 available from Tocris Bioscience    About this table
    CompoundAction CAS #
    BestatinAminopeptidase inhibitor[58970-76-6]
    SC 57461ASelective inhibitor of LTA4 hydrolase[423169-68-0]
    CHR 2797Aminopeptidase inhibitor[238750-77-1]

    1 HMDB Compound for METAP2    About this table
    CompoundSynonyms CAS #PubMed Ids
    CobaltCo (see all 6)7440-48-4--

    Selected DrugBank Compounds for METAP2 (see all 16)    About this table
    CompoundSynonyms CAS #TypeActionsPubMed Ids
    L-Methionine(S)-2-amino-4-(methylthio)butanoic acid (see all 6)63-68-3targetproduct of17114291 16540317 16917013 16823043 16789740
    (2s,3r)-3-Amino-2-Hydroxy-5-(Ethylsulfanyl)Pentanoyl-((S)-(-)-(1-Naphthyl)Ethyl)Amide-- --target--17139284 17016423 10592235
    N'-(2s,3r)-3-Amino-4-Cyclohexyl-2-Hydroxy-Butano-N-(4-Methylphenyl)Hydrazide-- --target--17139284 17016423 10592235
    Ovalicin-- --target--17139284 17016423 10592235
    (E)-(2r,3r,4s,5r)-3,4,5-Trihydroxy-2-Methoxy-8,8-Dimethyl-Non-6-Enoic Acid ((3s,6r)-6-Hydroxy-2-Oxo-Azepan-3-Yl)-Amide-- --target--17139284 17016423
    2-Methyl-2-Propanol-- --target--17139284 17016423
    3,5,6,8-Tetramethyl-N-Methyl Phenanthrolinium-- --target--17139284 17016423
    D-Methionine-- 348-67-4target--17139284 17016423
    (CHLOROACETYL)CARBAMIC ACID (3R,4S,5S,5R)-5-METHOXY-4-[(2R,3R)-2-METHYL-3-(3-METHYL-2-BUTENYL)OXIRANYL]-1-OXASPIRO[2.5]OCT-6-YL ESTER-- --target--10592235
    2-[(PHENYLSULFONYL)AMINO]-5,6,7,8-TETRAHYDRONAPHTHALENE-1-CARBOXYLIC ACID-- --target--10592235

    Selected Novoseek inferred chemical compound relationships for METAP2 gene (see all 11)    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    fumagillin 97.2 64 9860943 (7), 10760954 (6), 9177176 (4), 19246010 (3) (see all 24)
    ovalicin 97.2 17 9860943 (3), 14676204 (3), 9224570 (2), 16386852 (1) (see all 6)
    methionine 82.1 70 9177176 (3), 10574784 (3), 15102683 (2), 15760654 (2) (see all 36)
    triazole 45.4 3 15516829 (2)
    benzimidazole 42.4 1 15954858 (1)
    cobalt 41.2 10 15516829 (3), 17636946 (1), 12718546 (1)
    epoxide 30 5 9860943 (2), 9812898 (1)
    vegf 19.4 2 15927358 (1)
    manganese 12.6 7 12718546 (3), 15516829 (1)
    leucine 5.02 2 14741843 (1), 15187415 (1)



    Find genes that share compounds with METAP2           About GenesLikeMe



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, and/or Addgene, Primers from OriGene, and/or QIAGEN, Flow cytometry from eBioscience )
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    REFSEQ mRNAs for METAP2 gene: 
    NM_006838.3  

    Unigene Cluster for METAP2:

    Methionyl aminopeptidase 2
    Hs.444986  [show with all ESTs]
    Unigene Representative Sequence: NM_006838
    11 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000535095 ENST00000323666(uc001tec.3 uc010suv.2 uc001tef.3 uc001tee.3)
    ENST00000546753 ENST00000261220 ENST00000549502 ENST00000553151 ENST00000550777
    ENST00000551840 ENST00000549136 ENST00000549808 ENST00000546478
    miRNA
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    Block miRNA regulation of human, mouse, rat METAP2 using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate METAP2 (see all 42):
    hsa-miR-323-3p hsa-miR-29a hsa-miR-30d hsa-miR-548l hsa-miR-124 hsa-miR-30a hsa-miR-4330 hsa-miR-506
    SwitchGear 3'UTR luciferase reporter plasmidMETAP2 3' UTR sequence
    Inhib. RNA
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    Predesigned siRNA for gene silencing in human, mouse, rat METAP2
    Clone
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    OriGene ORF clones in mouse, rat for METAP2
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector: METAP2 (NM_006838)
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for METAP2
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat METAP2
    Primer
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    OriGene qPCR primer pairs and template standards for METAP2
    OriGene qSTAR qPCR primer pairs in human, mouse for METAP2
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat METAP2
      QuantiTect SYBR Green Assays in human, mouse, rat METAP2
      QuantiFast Probe-based Assays in human, mouse, rat METAP2

    Additional mRNA sequence: 

    AK055692.1 AK091730.1 AK300836.1 AK315559.1 BC013782.1 BC016008.1 U13261.1 

    18 DOTS entries:

    DT.218617  DT.95322384  DT.218616  DT.101983018  DT.100806161  DT.91771436  DT.100737178  DT.100762758 
    DT.100828121  DT.100828123  DT.100828129  DT.121187177  DT.121187183  DT.121187197  DT.121187202  DT.95322390 
    DT.101962101  DT.121187200 

    Selected AceView cDNA sequences (see all 407):

    BM971045 AW105130 AI753277 BX350057 BM460654 CB131136 BM845652 BP355466 
    BM842746 BP366001 AI268912 BU178806 AI656631 BQ775743 AU119930 N89839 
    BX480780 CR601954 AA909437 BP363353 CB157121 AU077170 BM667553 AA283030 

    GeneLoc Exon Structure

    Selected Alternative Splicing Database (ASD) splice patterns (SP) for METAP2 (see all 6)    About this scheme

    ExUns: 1a · 1b ^ 2 ^ 3 ^ 4 ^ 5 ^ 6a · 6b ^ 7a · 7b ^ 8 ^ 9 ^ 10 ^ 11 ^ 12 ^ 13
    SP1:                    -     -     -     -                                                         
    SP2:                                -     -                                                         
    SP3:                                      -                                                         
    SP4:                                                                                                
    SP5:                                -     -     -                                                   


    ECgene alternative splicing isoforms for METAP2

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

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    METAP2 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: --
    METAP2 Expression
    About this image


    METAP2 expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database
     selected tissues (see all 8) fully expand
     
     Brain (Nervous System)    fully expand to see all 4 entries
             Cerebral Cortex
     
     Testis (Reproductive System)
             Leydig Cells Testis Interstitium
     
     Bone (Muscoskeletal System)
             Bone Marrow
     
     Trophoblast (Extraembryonic Tissues)
             Trophoblast Cells Trophoblast
     
     Ovary (Reproductive System)
             Oviduct
    METAP2 Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    METAP2 Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.444986
        Pathway & Disease-focused RT2 Profiler PCR Arrays including METAP2: 
              Tumor Metastasis in human mouse rat
              Molecular Toxicology PathwayFinder 384HT in human mouse rat

    Primer
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    OriGene qPCR primer pairs and template standards for METAP2
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    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for METAP2

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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    This gene was present in the common ancestor of eukaryotes.

    Orthologs for METAP2 gene from Selected species (see all 29)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Metap21 , 5 methionine aminopeptidase 21, 5 89.19(n)1
    94.98(a)1
      10 (48.69 cM)5
    563071  NM_019648.31  NP_062622.11 
     938584895 
    chicken
    (Gallus gallus)
    Aves METAP21 methionyl aminopeptidase 2 80.56(n)
    87.37(a)
      417912  NM_001006233.1  NP_001006233.1 
    lizard
    (Anolis carolinensis)
    Reptilia METAP26
    methionyl aminopeptidase 2
    71(a)
    1 ↔ 1
    5(30789289-30870940)
    African clawed frog
    (Xenopus laevis)
    Amphibia metap2-prov2 methionine aminopeptidase 2 81.44(n)    BC043889.1 
    zebrafish
    (Danio rerio)
    Actinopterygii wufb98h062 wufb98h06 75.67(n)   323452  BC055563.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta und1 , 3 methionyl aminopeptidase3
    uninitiated1
    74(a)3
    63.94(n)1
    74.93(a)1
      30C73
    342941  NM_058025.41  NP_477373.11 
    worm
    (Caenorhabditis elegans)
    Secernentea Y116A8A.93   -- 73(a)
    (best of 3)
      IV(16848642-16855041)   --
    baker's yeast
    (Saccharomyces cerevisiae)
    Saccharomycetes MAP2(YBL091C)4
    MAP21
    Methionine aminopeptidase, catalyzes the cotranslational more4
    MAP21
    61.08(n)1
    60.51(a)1
      2(48628-47363)4
    8521871, 4  NP_009462.21  NP_009462.14 
    thale cress
    (Arabidopsis thaliana)
    eudicotyledons MAP2B1 MAP2B 65.01(n)
    73.48(a)
      825169  NM_001203210.1  NP_001190139.1 
    rice
    (Oryza sativa)
    Liliopsida Os.123222 Oryza sativa (japonica cultivar-group) cDNA cloneJ more 72.81(n)    AK122063.1 


    ENSEMBL Gene Tree for METAP2 (if available)
    TreeFam Gene Tree for METAP2 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
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    Paralogs for METAP2 gene
    ENSG000002574112  PA2G42  
    1 SIMAP similar gene for METAP2 using alignment to 11 protein entries:     MAP2_HUMAN (see all proteins):
    PA2G4

    Find genes that share paralogs with METAP2           About GenesLikeMe


    2 Pseudogenes.org Pseudogenes for METAP2
    PGOHUM00000240377 PGOHUM00000233201


    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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    Selected SNPs for METAP2 (see all 1234)    About this table                                 

    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 12 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs710820261,2
    C--92957000(-) AGAGT-/CTAA  
            
    CTTAG
    1 -- int11Minor allele frequency- CTAA:0.00NA 2
    rs792541321,2
    C,F--95865966(+) TCAGCC/GTAATT 1 -- us2k11Minor allele frequency- G:0.07WA 118
    rs756869771,2
    C,F--95865991(+) AAACTC/GTACCA 1 -- us2k11Minor allele frequency- G:0.07WA 118
    rs732293871,2
    C,F--95866062(+) GCTTTT/AATTAG 1 -- us2k11Minor allele frequency- A:0.01NA 120
    rs123117301,2
    C,F,H--95866069(+) TTAGCA/GTCATT 1 -- us2k1 tfbs313Minor allele frequency- G:0.11NS EA NA WA CSA 1487
    rs2002006741,2
    --95866183(+) ATGGA-/TGG   
       ATGAG
    TGGAT
    1 -- us2k10--------
    rs1463419311,2
    --95866195(+) AGTGAA/GTAAGT 1 -- us2k10--------
    rs1132513671,2
    C--95866199(+) AATAAG/ATGAAT 1 -- us2k11Minor allele frequency- A:0.50NA 2
    rs122985601,2
    C,F,H--95866257(+) GAAATG/CCTCCA 1 -- us2k16Minor allele frequency- C:0.04NS EA CSA WA 539
    rs1421911491,2
    --95866420(+) TTACCA/CACTTT 1 -- us2k10--------

    HapMap Linkage Disequilibrium report for METAP2 (95867296 - 95909615 bp)

    Structural Variations
         Database of Genomic Variants (DGV) 1 variation for METAP2:    About this table    
    Variant IDTypeSubtypePubMed ID
    nsv7234OTHER Inversion18451855

    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing METAP2
    DNA2.0 Custom Variant and Variant Library Synthesis for METAP2

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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    OMIM gene information: 601870    OMIM disorders: --

    1 disease for METAP2:    
    About MalaCards
    microsporidiosis

    1 disease from the University of Copenhagen DISEASES database for METAP2:
    Microsporidiosis

    Find genes that share disorders with METAP2           About GenesLikeMe

    5 Novoseek inferred disease relationships for METAP2 gene    About this table

    Disease   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    microsporidiosis 75.8 2 16917013 (1), 16004378 (1)
    cancer 23.5 14 18264137 (4), 16386852 (2), 16789740 (1), 15523682 (1) (see all 7)
    colon cancer 22.7 4 16386852 (1), 19563408 (1), 15336565 (1), 19703310 (1)
    tumors 11.3 23 18264137 (4), 17450475 (3), 15102683 (2), 16267007 (2) (see all 7)
    colorectal cancer 3.45 1 16386852 (1)

    Genetic Association Database (GAD): METAP2
    Human Genome Epidemiology (HuGE) Navigator: METAP2 (1 document)

    Export disorders for METAP2 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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    PubMed articles for METAP2 gene, integrated from 10 sources (see all 119):
    (articles sorted by number of sources associating them with METAP2)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Eukaryotic methionyl aminopeptidases: two classes of cobalt-dependent enzymes. (PubMed id 7644482)1, 2, 3, 9 Arfin S.M.... Bradshaw R.A. (Proc. Natl. Acad. Sci. U.S.A. 1995)
    2. Physiologically relevant metal cofactor for methionine aminopeptidase-2 is manganese. (PubMed id 12718546)1, 2, 9 Wang J.... Henkin J. (Biochemistry 2003)
    3. Structure of human methionine aminopeptidase-2 complexed with fumagillin. (PubMed id 9812898)1, 2, 9 Liu S.... Clardy J. (Science 1998)
    4. Evidence that the human homologue of a rat initiation factor-2 associated protein (p67) is a methionine aminopeptidase. (PubMed id 8858118)1, 3, 9 Li X. and Chang Y.H. (Biochem. Biophys. Res. Commun. 1996)
    5. Molecular cloning of a human complementary DNA encoding an initiation factor 2-associated protein (p67). (PubMed id 7873610)1, 2, 9 Li X. and Chang Y. (Biochim. Biophys. Acta 1995)
    6. Protein N-terminal processing: substrate specificity of Escherichia coli and human methionine aminopeptidases. (PubMed id 20521764)1, 2 Xiao Q.... Pei D. (Biochemistry 2010)
    7. Genetic variation and antioxidant response gene expression in the bronchial airway epithelium of smokers at risk for lung cancer. (PubMed id 20689807)1, 4 Wang X....Bell D.A. (PLoS ONE 2010)
    8. Human methionine aminopeptidase type 2 in complex with L- and D- methionine. (PubMed id 16540317)2, 7 Nonato M.C.... Clardy J. (Bioorg. Med. Chem. Lett. 2006)
    9. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (Nat. Genet. 2004)
    10. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S.... Malek J. (Genome Res. 2004)

    (in PubMed, OMIM, and NCBI Bookshelf)
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     ANDOR
    Aliases
    Disorders
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Entrez Gene: 10988 HGNC: 16672 AceView: METAP2 Ensembl:ENSG00000111142 euGenes: HUgn10988
    ECgene: METAP2 H-InvDB: METAP2

    (According to HUGE)
    About This Section

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      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
    About This Section

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    NameDescription
    PharmGKB entry for METAP2 Pharmacogenomics, SNPs, Pathways
    ATLAS Chromosomes in Cancer entry for METAP2 Genetics and Cytogenetics in Oncology and Haematology

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section

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    Patent Information for METAP2 gene:
    Search GeneIP for patents involving METAP2

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, eBioscience, and/or others, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Addgene, Cell lines from GenScript, and ESI BIO, Flow cytometery from eBioscience, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
    Advanced Cell Diagnostics, Animal models from inGenious Targeting Laboratory, genOway)
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