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MELK Gene

protein-coding   GIFtS: 66
GCID: GC09P036562

Maternal Embryonic Leucine Zipper Kinase

  Search for MELK
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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Aliases
Maternal Embryonic Leucine Zipper Kinase1 2     KIAA01753 5
Protein Kinase Eg32 3     HPK382
Protein Kinase PK382 3     EC 2.7.10.23
Tyrosine-Protein Kinase MELK2 3     hMELK3
PEg3 Kinase2 3     hPK383
EC 2.7.11.13 8     EC 2.7.118

External Ids:    HGNC: 168701   Entrez Gene: 98332   Ensembl: ENSG000001653047   OMIM: 6070255   UniProtKB: Q146803   

Export aliases for MELK gene to outside databases

Previous GC identifers: GC09P036885 GC09P036742


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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GeneCards Summary for MELK Gene:
MELK (maternal embryonic leucine zipper kinase) is a protein-coding gene. GO annotations related to this gene include lipid binding and calcium ion binding. An important paralog of this gene is STK40.

UniProtKB/Swiss-Prot: MELK_HUMAN, Q14680
Function: Serine/threonine-protein kinase involved in various processes such as cell cycle regulation,
self-renewal of stem cells, apoptosis and splicing regulation. Has a broad substrate specificity; phosphorylates
BCL2L14, CDC25B, MAP3K5/ASK1 and ZNF622. Acts as an activator of apoptosis by phosphorylating and activating
MAP3K5/ASK1. Acts as a regulator of cell cycle, notably by mediating phosphorylation of CDC25B, promoting
localization of CDC25B to the centrosome and the spindle poles during mitosis. Plays a key role in cell
proliferation and carcinogenesis. Required for proliferation of embryonic and postnatal multipotent neural
progenitors. Phosphorylates and inhibits BCL2L14, possibly leading to affect mammary carcinogenesis by mediating
inhibition of the pro-apoptotic function of BCL2L14. Also involved in the inhibition of spliceosome assembly
during mitosis by phosphorylating ZNF622, thereby contributing to its redirection to the nucleus. May also play a
role in primitive hematopoiesis

Gene Wiki entry for MELK Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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RefSeq DNA sequence at NCBI GenBank:
NC_000009.11  NC_018920.2  NT_008413.19  
Regulatory elements:
   Regulatory transcription factor binding sites in the MELK gene promoter:
         E2F-1   E2F   NF-kappaB2   RelA   Ik-3   NF-kappaB1   NF-kappaB   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidMELK promoter sequence
   Search Chromatin IP Primers for MELK

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat MELK


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 9p13.2   Ensembl cytogenetic band:  9p13.2   HGNC cytogenetic band: 9p13.1

MELK Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
MELK gene location

GeneLoc information about chromosome 9         GeneLoc Exon Structure

GeneLoc location for GC09P036562:  view genomic region     (about GC identifiers)

Start:
36,572,859 bp from pter      End:
36,677,679 bp from pter
Size:
104,821 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, Cloud-Clone Corp., and/or eBioscience,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, Cloud-Clone Corp., and/or eBioscience, Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, Cloud-Clone Corp, and/or others.)
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UniProtKB/Swiss-Prot: MELK_HUMAN, Q14680 (See protein sequence)
Recommended Name: Maternal embryonic leucine zipper kinase  
Size: 651 amino acids; 74642 Da
Subunit: Monomer. Interacts with ZNF622 and PPP1R8
Developmental stage: Increases during G2/M phase compared to interphase. Protein level decreases when cells exit
mitosis, probably due to degradation
Miscellaneous: Potential therapeutic target for treatment of somatic tumors, such as brain and breast cancers,
down-regulation of MELK inhibiting tumorigenesis (PubMed:17960622, PubMed:20861186)
Sequence caution: Sequence=BAA11492.2; Type=Erroneous initiation; Note=Translation N-terminally shortened;
4 PDB 3D structures from and Proteopedia for MELK:
4BKY (3D)        4BKZ (3D)        4BL1 (3D)        4IXP (3D)    
Secondary accessions: A6P3A7 A6P3A8 B1AMQ6 B7Z1E6 B7Z5M5 B7Z6Q7 B7Z6R8 B7Z6Y0 B7Z7Q1 D3DRP8
F5H0Y0 F5H2R4 F5H689 Q7L3C3
Alternative splicing: 8 isoforms:  Q14680-1   Q14680-2   Q14680-3   Q14680-4   Q14680-5   Q14680-6   Q14680-7   Q14680-8   
(No experimental confirmation available)

Explore the universe of human proteins at neXtProt for MELK: NX_Q14680

Explore proteomics data for MELK at MOPED

Post-translational modifications: 

  • Autophosphorylated: autophosphorylation of the T-loop at Thr-167 and Ser-171 is required for activation. Thr-478
    phosphorylation during mitosis promotes interaction with PPP1R8 (Probable)1
  • Ubiquitination2 at Lys44, Lys395, Lys406, Lys434, Lys536, Lys597, Lys650
  • Modification sites at PhosphoSitePlus
  • Selected DME Specific Peptides for MELK (Q14680) (see all 5)
     FRQIVSA  RQIVSAV  FAKVKLA  LKPENLL 


    See MELK Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins (9 alternative transcripts): 
    NP_001243614.1  NP_001243616.1  NP_001243617.1  NP_001243618.1  NP_001243619.1  NP_001243620.1  NP_001243621.1  NP_001243622.1  
    NP_055606.1  

    ENSEMBL proteins: 
     ENSP00000298048   ENSP00000445452   ENSP00000439792   ENSP00000437804   ENSP00000443550  
     ENSP00000441596   ENSP00000439184   ENSP00000438226  

    MELK Human Recombinant Protein Products:

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    Novus Biologicals MELK Proteins
    Novus Biologicals MELK Lysate
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    Browse Sino Biological Cell Lysates
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    Cloud-Clone Corp. Proteins for MELK

     
    Search eBioscience for Proteins for MELK 

    MELK Antibody Products:

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    MELK Assay Products:

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    Cloud-Clone Corp. ELISAs for MELK
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    Search eBioscience for ELISAs for MELK 


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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    IUPHAR Guide to PHARMACOLOGY protein family classification: maternal embryonic leucine zipper kinase
    MELK subfamily

    Selected InterPro protein domains (see all 7):
     IPR017441 Protein_kinase_ATP_BS
     IPR001772 KA1_dom
     IPR002290 Ser/Thr_dual-sp_kinase_dom
     IPR011009 Kinase-like_dom
     IPR008271 Ser/Thr_kinase_AS

    Graphical View of Domain Structure for InterPro Entry Q14680

    ProtoNet protein and cluster: Q14680

    1 Blocks protein domain: IPB001772 Kinase-associated

    UniProtKB/Swiss-Prot: MELK_HUMAN, Q14680
    Domain: The KA1 domain mediates binding to phospholipids and targeting to membranes (By similarity)
    Similarity: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily
    Similarity: Contains 1 KA1 (kinase-associated) domain
    Similarity: Contains 1 protein kinase domain


    Find genes that share domains with MELK           About GenesLikeMe


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, Vector BioLabs and/or Addgene, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Flow cytometry from eBioscience, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Molecular Function:

         UniProtKB/Swiss-Prot Summary: MELK_HUMAN, Q14680
    Function: Serine/threonine-protein kinase involved in various processes such as cell cycle regulation,
    self-renewal of stem cells, apoptosis and splicing regulation. Has a broad substrate specificity; phosphorylates
    BCL2L14, CDC25B, MAP3K5/ASK1 and ZNF622. Acts as an activator of apoptosis by phosphorylating and activating
    MAP3K5/ASK1. Acts as a regulator of cell cycle, notably by mediating phosphorylation of CDC25B, promoting
    localization of CDC25B to the centrosome and the spindle poles during mitosis. Plays a key role in cell
    proliferation and carcinogenesis. Required for proliferation of embryonic and postnatal multipotent neural
    progenitors. Phosphorylates and inhibits BCL2L14, possibly leading to affect mammary carcinogenesis by mediating
    inhibition of the pro-apoptotic function of BCL2L14. Also involved in the inhibition of spliceosome assembly
    during mitosis by phosphorylating ZNF622, thereby contributing to its redirection to the nucleus. May also play a
    role in primitive hematopoiesis
    Catalytic activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
    Catalytic activity: ATP + a protein = ADP + a phosphoprotein
    Enzyme regulation: Activated by autophosphorylation of the T-loop at Thr-167 and Ser-171: in contrast to other
    members of the SNF1 subfamily, phosphorylation at Thr-167 is not mediated by STK11/LKB1 but via
    autophosphorylation instead. Inhibited by calcium-binding. Kinase activity is also regulated by reducing agents:
    dithiothreitol (DTT) or reduced glutathione are required for kinase activity in vitro; such dependence is however
    not due to the presence of disulfide bonds
    Induction: Up-regulated in many cancers cells. Up-regulated upon treatment with radiation or 5-fluorouracil (5-FU)
    in colorectal cancer cells, suggesting that it might be associated with increased resistance of colorectal cells
    against radiation and 5-FU. Down-regulated upon siomycin A, a thiazole antibiotic, treatment, leading to inhibit
    tumor growth in vivo

         Enzyme Numbers (IUBMB): EC 2.7.11.11 2 EC 2.7.112 EC 2.7.10.21

         Gene Ontology (GO): Selected molecular function terms (see all 10):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0004672protein kinase activity ----
    GO:0004674protein serine/threonine kinase activity IDA12400006
    GO:0004713protein tyrosine kinase activity ----
    GO:0004715non-membrane spanning protein tyrosine kinase activity IDA16216881
    GO:0005509calcium ion binding IDA16216881
         
    Find genes that share ontologies with MELK           About GenesLikeMe


    Phenotypes:
         3 GenomeRNAi human phenotypes for MELK:
     G0/1 arrest  Increased G1 DNA content  Increased cell death HMECs cel 

    Animal Models:
       inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for MELK
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       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for MELK

    miRNA
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    miRTarBase miRNAs that target MELK:
    hsa-mir-193b-3p (MIRT016391)

    Block miRNA regulation of human, mouse, rat MELK using miScript Target Protectors
    1 qRT-PCR Assays for microRNA that regulate MELK:
    hsa-miR-205*
    SwitchGear 3'UTR luciferase reporter plasmidMELK 3' UTR sequence
    Inhib. RNA
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    Predesigned siRNA for gene silencing in human, mouse, rat MELK

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    GenScript: all cDNA clones in your preferred vector: MELK (NM_014791)
    Browse Sino Biological Human cDNA Clones
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for MELK
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat MELK

    Cell Line
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    In Situ Assay
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    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for MELK


    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Subcellular locations from UniProtKB/Swiss-Prot
    MELK_HUMAN, Q14680: Cell membrane; Peripheral membrane protein
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    plasma membrane5
    cytosol3
    endoplasmic reticulum1
    golgi apparatus1
    mitochondrion1
    nucleus1

    Gene Ontology (GO): 4 cellular component terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005737cytoplasm ----
    GO:0005886plasma membrane IDA--
    GO:0005938cell cortex IDA16159311
    GO:0016020membrane ----

    Find genes that share ontologies with MELK           About GenesLikeMe


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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    SuperPaths for MELK About    
    See pathways by source

    SuperPathContained pathways About
    1Neuroscience
    Neuroscience


    Find genes that share SuperPaths with MELK           About GenesLikeMe

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways


    1 Cell Signaling Technology (CST) Pathway for MELK
        Neuroscience


        Custom Pathway & Disease-focused RT2 Profiler PCR Arrays for MELK
    Interactions:

        GeneGlobe Interaction Network for MELK

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    Selected Interacting proteins for MELK (Q146801, 2, 3 ENSP000002980484) via UniProtKB, MINT, STRING, and/or I2D (see all 576)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    SF3B1O755332, 3, ENSP000003353214MINT-7947479 I2D: score=2 STRING: ENSP00000335321
    CDC5LQ994592, 3, ENSP000003605324MINT-7947479 I2D: score=1 STRING: ENSP00000360532
    BCL2L14Q9BZR81, 3, ENSP000003091324EBI-1046702,EBI-1385773 I2D: score=1 STRING: ENSP00000309132
    BABAM1Q9NWV83, ENSP000003524084I2D: score=2 STRING: ENSP00000352408
    ZNF622Q969S33, ENSP000003100424I2D: score=2 STRING: ENSP00000310042
    About this table

    Gene Ontology (GO): Selected biological process terms (see all 11):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000086G2/M transition of mitotic cell cycle TAS12400006
    GO:0006468protein phosphorylation ----
    GO:0006915apoptotic process IDA17280616
    GO:0008283cell proliferation IDA17280616
    GO:0008631intrinsic apoptotic signaling pathway in response to oxidative stress IEA--

    Find genes that share ontologies with MELK           About GenesLikeMe



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Browse Tocris compounds for MELK

    2 HMDB Compounds for MELK    About this table
    CompoundSynonyms CAS #PubMed Ids
    ADPadenosindiphosphorsaeure (see all 8)58-64-0--
    Adenosine triphosphate5'-(tetrahydrogen triphosphate) Adenosine (see all 24)56-65-5--

    1 Novoseek inferred chemical compound relationship for MELK gene    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    leucine 69.8 11 17280616 (2), 17722061 (2), 17960622 (2), 16216881 (1) (see all 8)



    Find genes that share compounds with MELK           About GenesLikeMe



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, and/or Addgene, Primers from OriGene, and/or QIAGEN, Flow cytometry from eBioscience )
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    REFSEQ mRNAs for MELK gene (9 alternative transcripts): 
    NM_001256685.1  NM_001256687.1  NM_001256688.1  NM_001256689.1  NM_001256690.1  NM_001256691.1  NM_001256692.1  NM_001256693.1  
    NM_014791.3  

    Unigene Cluster for MELK:

    Maternal embryonic leucine zipper kinase
    Hs.184339  [show with all ESTs]
    Unigene Representative Sequence: NR_046337
    12 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000298048 ENST00000489766 ENST00000495529 ENST00000487398 ENST00000480021
    ENST00000545008(uc011lpr.2) ENST00000536860(uc011lpp.2) ENST00000541717(uc011lpn.2)
    ENST00000536329(uc010mlm.3) ENST00000543751(uc003zzn.3 uc011lpo.2 uc010mll.3 uc011lpq.2 uc011lps.2)
    ENST00000536987(uc011lpm.2) ENST00000538311(uc022bgq.1)
    miRNA
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    Inhib. RNA
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      QuantiFast Probe-based Assays in human, mouse, rat MELK

    Additional mRNA sequence: 

    AB183427.1 AB183428.1 AB267395.1 AK222811.1 AK293284.1 AK293423.1 AK293447.1 AK296820.1 
    AK299164.1 AK300761.1 AK300821.1 AK301131.1 AK302374.1 AK309470.1 BC014039.2 D79997.1 
    NR_046337.1 

    12 DOTS entries:

    DT.212642  DT.121191399  DT.121191407  DT.121191419  DT.91711226  DT.100777414  DT.100777416  DT.102828545 
    DT.91742966  DT.97843505  DT.97843506  DT.100777415 

    Selected AceView cDNA sequences (see all 99):

    BU622206 BC014039 BM787111 BM823308 CR605252 AA835959 D79997 AL135150 
    BM927868 BQ064763 BM851510 NM_014791 AI637653 BQ650221 AA307070 AI753377 
    AU124892 BQ071315 CF141336 AL554470 AW949325 AA436897 BM760835 BE880179 

    GeneLoc Exon Structure

    Selected Alternative Splicing Database (ASD) splice patterns (SP) for MELK (see all 6)    About this scheme

    ExUns: 1a · 1b · 1c · 1d ^ 2 ^ 3 ^ 4 ^ 5 ^ 6a · 6b · 6c · 6d ^ 7 ^ 8 ^ 9 ^ 10 ^ 11 ^ 12 ^ 13 ^ 14 ^ 15 ^ 16 ^ 17 ^ 18 ^ 19 ^ 20
    SP1:                          -     -                             -     -                                                                                       
    SP2:                                -                             -     -                                                                                       
    SP3:                                -           -                 -     -                                                                                       
    SP4:                                                                                                                                                            
    SP5:                                -                                                                                                                           


    ECgene alternative splicing isoforms for MELK

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
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    MELK expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: TGGTTTTGTA
    MELK Expression
    About this image


    MELK expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database
     selected tissues (see all 10) fully expand
     
     Brain (Nervous System)    fully expand to see all 7 entries
             Endothelial Cells Blood Brain Barrier
             Cerebral Cortex
     
     Gut Tube (Gastrointestinal Tract)    fully expand to see all 3 entries
             Hindgut
     
     Neural Tube (Nervous System)    fully expand to see all 3 entries
             Telencephalon
     
     Lung (Respiratory System)    fully expand to see all 2 entries
             Stem Bronchi
     
     Endothelium (Cardiovascular System)    fully expand to see all 2 entries
             Endothelial Cells Blood Brain Barrier
    MELK Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    MELK Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.184339

    UniProtKB/Swiss-Prot: MELK_HUMAN, Q14680
    Tissue specificity: Expressed in placenta, kidney, thymus, testis, ovary and intestine

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    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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    This gene was present in the common ancestor of eukaryotes.

    Orthologs for MELK gene from Selected species (see all 18)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Melk1 , 5 maternal embryonic leucine zipper kinase1, 5 84.19(n)1
    84.29(a)1
      4 (23.46 cM)5
    172791  NM_010790.21  NP_034920.21 
     443008765 
    chicken
    (Gallus gallus)
    Aves MELK1 maternal embryonic leucine zipper kinase 67.7(n)
    62.89(a)
      428391  NM_001031509.1  NP_001026680.1 
    lizard
    (Anolis carolinensis)
    Reptilia MELK6
    maternal embryonic leucine zipper kinase
    59(a)
    1 ↔ 1
    GL343201.1(2878187-2907953)
    African clawed frog
    (Xenopus laevis)
    Amphibia LOC3979272 p69Eg3 76.18(n)    Z17205.2 
    zebrafish
    (Danio rerio)
    Actinopterygii wufb71b012 Danio rerio mRNA similar to maternal embryonic leucine more 76.33(n)    AB108827.1 
    worm
    (Caenorhabditis elegans)
    Secernentea pig-11 pig-1 49.56(n)
    43.06(a)
      176877  NM_001028249.3  NP_001023420.2 
    baker's yeast
    (Saccharomyces cerevisiae)
    Saccharomycetes SNF16
    AMP-activated serine/threonine protein kinase foun...
    23(a)
    1 → many
    IV(1412373-1414274) YDR477W
    thale cress
    (Arabidopsis thaliana)
    eudicotyledons CIPK71 CIPK7 50.91(n)
    44.53(a)
      821874  NM_113200.2  NP_188940.1 


    ENSEMBL Gene Tree for MELK (if available)
    TreeFam Gene Tree for MELK (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
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    Paralogs for MELK gene
    STK402  TRIB32  NUAK12  PRKAA22  HUNK2  TRIB12  NUAK22  PRKAA12  
    TRIB22  
    Selected SIMAP similar genes for MELK using alignment to 1 protein entry:     MELK_HUMAN(see all similar genes):
    MELKV4    PRKAA1    CAMK1D    PRKAA2    CAMK1    PSKH1
    TSSK3    PNCK    PRKY    PSKH2    TSSK4    AURKB
    PRKX    DAPK2    TSSK2    TSSK6    TSSK1    TSSK1B

    Find genes that share paralogs with MELK           About GenesLikeMe



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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    Selected SNPs for MELK (see all 2046)    About this table                                 

    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 9 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    VAR_0407984
    An ovarian mucinous carcinoma sample4--see VAR_0407982 T M mis40--------
    rs1398243571,2
    --36570894(+) CTTTGA/CGGGGT 10 -- us2k10--------
    rs1431285781,2
    --36571039(+) CTTACA/GGATGA 10 -- us2k10--------
    rs1865472461,2
    --36571063(+) GCTTTC/TTTGAG 10 -- us2k10--------
    rs1112576711,2
    C,F--36571381(+) AAAAGA/GTTATG 10 -- us2k12Minor allele frequency- G:0.04NA 122
    rs734369431,2
    C,F--36571422(+) ATTAAG/TTTTAG 10 -- us2k13Minor allele frequency- T:0.13WA CSA 122
    rs1113682491,2
    F--36571451(+) TGCATT/ATTAAG 10 -- us2k12Minor allele frequency- A:0.50CSA 4
    rs3293551,2
    C,F--36571473(-) AGAAaT/Gaaatt 10 -- us2k1 tfbs39Minor allele frequency- G:0.25NA WA CSA EA 368
    rs1897641291,2
    --36571498(+) TTCTCC/TCCCTT 10 -- us2k10--------
    rs1474128241,2
    C--36571512(+) TTCCT-/TTCCC 
            
    TTCCC
    10 -- us2k10--------

    HapMap Linkage Disequilibrium report for MELK (36572859 - 36677679 bp)

    Structural Variations
         Database of Genomic Variants (DGV) 5 variations for MELK:    About this table    
    Variant IDTypeSubtypePubMed ID
    esv2738428CNV Deletion23290073
    esv2667249CNV Deletion23128226
    esv272822CNV Insertion20981092
    nsv6530CNV Loss18451855
    nsv831550CNV Loss17160897

    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing MELK
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    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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    OMIM gene information: 607025    OMIM disorders: --

    UniProtKB/Swiss-Prot: MELK_HUMAN, Q14680
  • Note=Defects in MELK are associated with some cancers, such as brain or breast cancers. Expression is
    dramatically increased in aggressive undifferentiated tumors, correlating with poor patient outcome in breast and
    brain cancers, suggesting a role in tumor-initiating cells and proliferation via its function in cell
    proliferation regulation

  • 1 disease from the University of Copenhagen DISEASES database for MELK:
    Glioblastoma

    Find genes that share disorders with MELK           About GenesLikeMe

    Genetic Association Database (GAD): MELK

    Export disorders for MELK gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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    PubMed articles for MELK gene, integrated from 10 sources (see all 50):
    (articles sorted by number of sources associating them with MELK)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Prediction of the coding sequences of unidentified human genes. V. The coding sequences of 40 new genes (KIAA0161-KIAA0200) deduced by analysis of cDNA clones from human cell line KG-1. (PubMed id 8724849)1, 2, 3 Nagase T.... Nomura N. (DNA Res. 1996)
    2. Maternal embryonic leucine zipper kinase transcript abundance correlates with malignancy grade in human astrocytomas. (PubMed id 17960622)1, 2, 9 Marie S.K.... Caballero O.L. (Int. J. Cancer 2008)
    3. Maternal embryonic leucine zipper kinase is a key regulator of the proliferation of malignant brain tumors, including brain tumor stem cells. (PubMed id 17722061)1, 2, 9 Nakano I.... Kornblum H.I. (J. Neurosci. Res. 2008)
    4. Involvement of maternal embryonic leucine zipper kinase (MELK) in mammary carcinogenesis through interaction with Bcl-G, a pro-apoptotic member of the Bcl-2 family. (PubMed id 17280616)1, 2, 9 Lin M.L.... Katagiri T. (Breast Cancer Res. 2007)
    5. Human pEg3 kinase associates with and phosphorylates CDC25B phosphatase: a potential role for pEg3 in cell cycle regulation. (PubMed id 12400006)1, 2, 9 Davezac N.... Tassan J.P. (Oncogene 2002)
    6. Dysregulated expression of Fau and MELK is associated with poor prognosis in breast cancer. (PubMed id 19671159)1, 2, 9 Pickard M.R.... Williams G.T. (Breast Cancer Res. 2009)
    7. Substrate specificity and activity regulation of protein kinase MELK. (PubMed id 16216881)1, 2, 9 Beullens M.... Bollen M. (J. Biol. Chem. 2005)
    8. CDC25B phosphorylated by pEg3 localizes to the centrosome and the spindle poles at mitosis. (PubMed id 15908796)1, 2, 9 Mirey G.... Ducommun B. (Cell Cycle 2005)
    9. Inhibition of spliceosome assembly by the cell cycle-regulated protein kinase MELK and involvement of splicing factor NIPP1. (PubMed id 14699119)1, 2, 9 Vulsteke V.... Bollen M. (J. Biol. Chem. 2004)
    10. Siomycin A targets brain tumor stem cells partially through a MELK- mediated pathway. (PubMed id 21558073)1, 2 Nakano I.... Kornblum H.I. (Neuro-oncol. 2011)

    (in PubMed, OMIM, and NCBI Bookshelf)
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     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Entrez Gene: 9833 HGNC: 16870 AceView: MELK Ensembl:ENSG00000165304 euGenes: HUgn9833
    ECgene: MELK H-InvDB: MELK

    (According to HUGE)
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    HUGE: KIAA0175

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
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    NameDescription
    PharmGKB entry for MELK Pharmacogenomics, SNPs, Pathways
    ATLAS Chromosomes in Cancer entry for MELK Genetics and Cytogenetics in Oncology and Haematology

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
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    Patent Information for MELK gene:
    Search GeneIP for patents involving MELK

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



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