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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

MELK Gene

protein-coding   GIFtS: 61
GCID: GC09P036562

maternal embryonic leucine zipper kinase
 Explore 9 diseases affiliated with
MELK via
MalaCards
our new
 Human Malady Compendium 
Biological research products
for MELK    

Aliases
for MELK gene

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section
Aliases
Maternal Embryonic Leucine Zipper Kinase1 2     EC 2.7.11.13 8
KIAA01751 3 5     HPK382
Protein Kinase Eg32 3     EC 2.7.10.23
Protein Kinase PK382 3     HMELK1
Tyrosine-Protein Kinase MELK2 3     HPK382
PEg3 Kinase2 3     EC 2.7.118

External Ids:    HGNC: 168701   Entrez Gene: 98332   Ensembl: ENSG000001653047   OMIM: 6070255   UniProtKB: Q146803   

Export aliases for MELK gene to outside databases

Previous GC identifers: GC09P036885 GC09P036742


Summaries
for MELK gene

(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section
UniProtKB/Swiss-Prot: MELK_HUMAN, Q14680
Function: Serine/threonine-protein kinase involved in various processes such as cell cycle regulation, self-renewal of
stem cells, apoptosis and splicing regulation. Has a broad substrate specificity; phosphorylates BCL2L14, CDC25B,
MAP3K5/ASK1 and ZNF622. Acts as an activator of apoptosis by phosphorylating and activating MAP3K5/ASK1. Acts as a
regulator of cell cycle, notably by mediating phosphorylation of CDC25B, promoting localization of CDC25B to the
centrosome and the spindle poles during mitosis. Plays a key role in cell proliferation and carcinogenesis. Required
for proliferation of embryonic and postnatal multipotent neural progenitors. Phosphorylates and inhibits BCL2L14,
possibly leading to affect mammary carcinogenesis by mediating inhibition of the pro-apoptotic function of BCL2L14.
Also involved in the inhibition of spliceosome assembly during mitosis by phosphorylating ZNF622, thereby contributing
to its redirection to the nucleus. May also play a role in primitive hematopoiesis

Gene Wiki entry for MELK


Genomic Views
for MELK gene

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000009.11  NC_018920.1  NT_008413.18  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the MELK gene promoter:
         E2F-1   E2F   NF-kappaB2   RelA   Ik-3   NF-kappaB1   NF-kappaB   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmid: MELK promoter sequence
   Search SABiosciences Chromatin IP Primers for MELK

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat MELK


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 9p13.2   Ensembl cytogenetic band:  9p13.2   HGNC cytogenetic band: 9p13.1

MELK Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
MELK gene location

GeneLoc information about chromosome 9         GeneLoc Exon Structure

GeneLoc location for GC09P036562:  view genomic region     (about GC identifiers)

Start:
 36,572,859 bp from pter      End:
 36,677,679 bp from pter
Size:
 104,821 bases      Orientation:
 plus strand

Proteins
for MELK gene

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section
UniProtKB/Swiss-Prot: MELK_HUMAN, Q14680 (See protein sequence)
Recommended Name: Maternal embryonic leucine zipper kinase  
Size: 651 amino acids; 74642 Da
Subunit: Monomer. Interacts with ZNF622 and PPP1R8
Subcellular location: Cell membrane; Peripheral membrane protein
Developmental stage: Increases during G2/M phase compared to interphase. Protein level decreases when cells exit
mitosis, probably due to degradation
Miscellaneous: Potential therapeutic target for treatment of somatic tumors, such as brain and breast cancers,
down-regulation of MELK inhibiting tumorigenesis (PubMed:17960622, PubMed:20861186)
Sequence caution: Sequence=BAA11492.2; Type=Erroneous initiation; Note=Translation N-terminally shortened;
1 PDB 3D structure from and Proteopedia for MELK:
3ZGW (3D)    
Secondary accessions: A6P3A7 A6P3A8 B1AMQ6 B7Z1E6 B7Z6R8 B7Z6Y0 B7Z7Q1 D3DRP8 F5H2R4 Q7L3C3
Alternative splicing: 6 isoforms:  Q14680-1   Q14680-2   Q14680-3   Q14680-4   Q14680-5   Q14680-6   (No experimental confirmation available)

Explore the universe of human proteins at neXtProt for MELK: NX_Q14680

Post-translational modifications:

  • Autophosphorylated: autophosphorylation of the T-loop at Thr-167 and Ser-171 is required for activation. Thr-478
    • phosphorylation during mitosis promotes interaction with PPP1R8 (Probable)1
  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_Q14680

    • 4/5 DME Specific Peptides for MELK (Q14680) (see all 5)
     FRQIVSA  RQIVSAV  FAKVKLA  LKPENLL 

    MELK Protein expression data from MOPED and PaxDb:    About this image 
    Estimated protein expression log10 (pmol).

    REFSEQ proteins (9 alternative transcripts): 
    NP_001243614.1  NP_001243616.1  NP_001243617.1  NP_001243618.1  NP_001243619.1  NP_001243620.1  NP_001243621.1  NP_001243622.1  
    NP_055606.1  

    ENSEMBL proteins: 
     ENSP00000298048   ENSP00000445452   ENSP00000439792   ENSP00000437804   ENSP00000443550  
     ENSP00000441596   ENSP00000439184   ENSP00000438226  

    Human Recombinant Protein Products: 
    EMD Millipore Purified and/or Recombinant MELK Protein
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    Novus Biologicals MELK Proteins
    Novus Biologicals MELK Lysate
    Browse Sino Biological Recombinant Proteins
    Browse ProSpec Recombinant Proteins
    Uscn Proteins for MELK

    Gene Ontology (GO): 3 cellular component terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005737cytoplasm ----
    GO:0005886plasma membrane IDA--
    GO:0005938cell cortex IDA16159311


    MELK for ontologies           About GeneDecksing



    MELK Antibody Products: 
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    ThermoFisher Antibodies for MELK

    Assay Products for MELK: 
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    Browse R&D Systems for biochemical assays
    GenScript MELK-Activity-based Kinase Assay for Compound Screening
    Browse Enzo Life Sciences for kits & assays
    Uscn ELISAs and CLIAs for MELK

    Protein Domains /
    Families
    for MELK gene

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section
    MELK for domains           About GeneDecksing

    5/6 InterPro domains/families (see all 6):
     IPR017441 Protein_kinase_ATP_BS
     IPR001772 KA1_dom
     IPR002290 Ser/Thr_dual-sp_kinase_dom
     IPR011009 Kinase-like_dom
     IPR008271 Ser/Thr_kinase_AS

    Graphical View of Domain Structure for InterPro Entry Q14680

    ProtoNet protein and cluster: Q14680

    1 Blocks protein family: IPB001772 Kinase-associated

    UniProtKB/Swiss-Prot: MELK_HUMAN, Q14680
    Domain: The KA1 domain mediates binding to phospholipids and targeting to membranes (By similarity)
    Similarity: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily
    Similarity: Contains 1 KA1 (kinase-associated) domain
    Similarity: Contains 1 protein kinase domain


    Function
    for MELK gene

    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section
    Function Summary:

         UniProtKB/Swiss-Prot: MELK_HUMAN, Q14680
    Function: Serine/threonine-protein kinase involved in various processes such as cell cycle regulation, self-renewal of
    stem cells, apoptosis and splicing regulation. Has a broad substrate specificity; phosphorylates BCL2L14, CDC25B,
    MAP3K5/ASK1 and ZNF622. Acts as an activator of apoptosis by phosphorylating and activating MAP3K5/ASK1. Acts as a
    regulator of cell cycle, notably by mediating phosphorylation of CDC25B, promoting localization of CDC25B to the
    centrosome and the spindle poles during mitosis. Plays a key role in cell proliferation and carcinogenesis. Required
    for proliferation of embryonic and postnatal multipotent neural progenitors. Phosphorylates and inhibits BCL2L14,
    possibly leading to affect mammary carcinogenesis by mediating inhibition of the pro-apoptotic function of BCL2L14.
    Also involved in the inhibition of spliceosome assembly during mitosis by phosphorylating ZNF622, thereby contributing
    to its redirection to the nucleus. May also play a role in primitive hematopoiesis
    Catalytic activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
    Catalytic activity: ATP + a protein = ADP + a phosphoprotein
    Enzyme regulation: Activated by autophosphorylation of the T-loop at Thr-167 and Ser-171: in contrast to other members
    of the SNF1 subfamily, phosphorylation at Thr-167 is not mediated by STK11/LKB1 but via autophosphorylation instead.
    Inhibited by calcium-binding. Kinase activity is also regulated by reducing agents: dithiothreitol (DTT) or reduced
    glutathione are required for kinase activity in vitro; such dependence is however not due to the presence of disulfide
    bonds
    Induction: Up-regulated in many cancers cells. Up-regulated upon treatment with radiation or 5-fluorouracil (5-FU) in
    colorectal cancer cells, suggesting that it might be associated with increased resistance of colorectal cells against
    radiation and 5-FU. Down-regulated upon siomycin A, a thiazole antibiotic, treatment, leading to inhibit tumor growth
    in vivo

    Enzyme Numbers (IUBMB): EC 2.7.11.11 2 EC 2.7.112 EC 2.7.10.21

    miRNA
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    QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat MELK
    1 QIAGEN miScript miRNA Assays for microRNA that regulate MELK:
    hsa-miR-205*
    SwitchGear 3'UTR luciferase reporter plasmid: MELK 3' UTR sequence
    Inhib. RNA
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    OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for MELK (see all 7)
    OriGene shRNA RFP: MELK
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    QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat MELK

    Gene Editing
    Products:    		DNA2.0 Custom Protein Engineering Service for MELK

    Clone
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    OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for MELK (see all 3)
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    Cell Line
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    Search LifeMap BioReagents cell lines for MELK

    In Situ Assay
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         		Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for MELK

    Gene Ontology (GO): 5/7 molecular function terms (GO ID links to tree view) (see all 7):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0004672protein kinase activity ----
    GO:0004674protein serine/threonine kinase activity IDA12400006
    GO:0004715non-membrane spanning protein tyrosine kinase activity IDA16216881
    GO:0005509calcium ion binding IDA16216881
    GO:0005515protein binding IPI17280616


    MELK for ontologies           About GeneDecksing


    3 GenomeRNAi human phenotypes for MELK:
     G0/1 arrest  Increased G1 DNA content  Increased cell death HMECs cel 


    Pathways & Interactions
    for MELK gene

    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section

    Unified GeneCards pathways  About this table 
    See pathways by source

    Super-pathwaycontained gene-specific pathways
    1Neuroscience
    		Neuroscience1.00

    Pathway sources
    See GeneCards unified pathways
    Show all pathways


    1 Cell Signaling Technology (CST) Pathway for MELK
        Neuroscience



    MELK for pathways           About GeneDecksing

    Interactions:

        SABiosciences Gene Network CentralTM Interacting Genes and Proteins Network for MELK

    STRING Interaction Network Preview (showing 5 interactants - click image to see 13)

    5/538 Interacting proteins for MELK (Q146801, 2, 3 ENSP000002980484) via UniProtKB, MINT, STRING, and/or I2D (see all 538)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    SF3B1O755332, 3, ENSP000003353214MINT-7947479 I2D: score=2 STRING: ENSP00000335321
    CDC5LQ994592, 3, ENSP000003605324MINT-7947479 I2D: score=1 STRING: ENSP00000360532
    BCL2L14Q9BZR81, 3, ENSP000003091324EBI-1046702,EBI-1385773 I2D: score=1 STRING: ENSP00000309132
    BABAM1Q9NWV83, ENSP000003524084I2D: score=2 STRING: ENSP00000352408
    ZNF622Q969S33, ENSP000003100424I2D: score=2 STRING: ENSP00000310042
    About this table

    Gene Ontology (GO): 5/8 biological process terms (GO ID links to tree view) (see all 8):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000086G2/M transition of mitotic cell cycle TAS12400006
    GO:0006468protein phosphorylation ----
    GO:0006915apoptotic process IDA17280616
    GO:0008283cell proliferation IDA17280616
    GO:0030097hemopoiesis ISS--


    MELK for ontologies           About GeneDecksing



    Drugs & Compounds
    for MELK gene

    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section

    MELK for compounds           About GeneDecksing

    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for MELK

    2 HMDB Compounds for MELK    About this table
    CompoundSynonyms CAS #PubMed Ids
    ADPadenosindiphosphorsaeure (see all 8)58-64-0--
    Adenosine triphosphate5'-(tetrahydrogen triphosphate) Adenosine (see all 24)56-65-5--
    1 Novoseek chemical compound relationship for MELK gene    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    leucine 69.8 11 17280616 (2), 17722061 (2), 17960622 (2), 16216881 (1) (see all 8)

    Search CenterWatch for drugs/clinical trials and news about MELK 

    Transcripts
    for MELK gene

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section
    REFSEQ mRNAs for MELK gene (9 alternative transcripts): 
    NM_001256685.1  NM_001256687.1  NM_001256688.1  NM_001256689.1  NM_001256690.1  NM_001256691.1  NM_001256692.1  NM_001256693.1  
    NM_014791.3  

    Unigene Cluster for MELK:

    Maternal embryonic leucine zipper kinase
    Hs.184339  [show with all ESTs]
    Unigene Representative Sequence: NR_046337
    12 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000298048 ENST00000489766 ENST00000495529 ENST00000487398 ENST00000480021
    ENST00000545008(uc011lpr.2) ENST00000536860(uc011lpp.2) ENST00000541717(uc011lpn.2)
    ENST00000536329(uc010mlm.3) ENST00000543751(uc003zzn.3 uc011lpo.2 uc010mll.3 uc011lpq.2 uc011lps.2)
    ENST00000536987(uc011lpm.2) ENST00000538311(uc022bgq.1)

    miRNA
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    Inhib. RNA
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    Clone
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    Additional cDNA sequence: 

    AB183427.1 AB183428.1 AB267395.1 AK222811.1 AK293284.1 AK293423.1 AK293447.1 AK296820.1 
    AK299164.1 AK300761.1 AK300821.1 AK301131.1 AK302374.1 AK309470.1 BC014039.2 D79997.1 
    NR_046337.1 

    12 DOTS entries:

    DT.212642  DT.121191399  DT.121191407  DT.121191419  DT.91711226  DT.100777414  DT.100777416  DT.102828545 
    DT.91742966  DT.97843505  DT.97843506  DT.100777415 

    24/99 AceView cDNA sequences (see all 99):

    BQ064763 AL135150 BM851510 BU622206 AA307070 BQ071315 AI637653 NM_014791 
    D79997 AU124892 CR605252 BQ650221 CF141336 AL554470 AI753377 AA835959 
    BM927868 BM787111 BC014039 BM823308 AU133832 BE543330 BP356223 BU150260 

    GeneLoc Exon Structure

    5/6 Alternative Splicing Database (ASD) splice patterns (SP) for MELK (see all 6)    About this scheme

    ExUns: 1a · 1b · 1c · 1d ^ 2 ^ 3 ^ 4 ^ 5 ^ 6a · 6b · 6c · 6d ^ 7 ^ 8 ^ 9 ^ 10 ^ 11 ^ 12 ^ 13 ^ 14 ^ 15 ^ 16 ^ 17 ^ 18 ^ 19 ^ 20
    SP1:                          -     -                             -     -                                                                                       
    SP2:                                -                             -     -                                                                                       
    SP3:                                -           -                 -     -                                                                                       
    SP4:                                                                                                                                                            
    SP5:                                -                                                                                                                           


    ECgene alternative splicing isoforms for MELK

    Expression
    for MELK gene

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    MELK expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: TGGTTTTGTA

    Microarray
    RNAseq (Illumina Body Map)
    (100×FPKM)½
    SAGE (Serial Analysis of Gene Expression)
    About this image

    MELK expression in embryonic tissues and stem cells
    Expression by the Database of Embryonic development, Stem cell research, and Regenerative medicine    About this table
    10 LifeMap In Vivo Development Anatomical Compartments/Cells 
    Tissue Anatomical Compartment CellCategory (developmental path)
    BrainBlood Brain BarrierEndothelial CellsBlood Brain Barrier, Endothelium
    BrainBlood Brain BarrierPostnatal Endothelial CellsBlood Brain Barrier, Endothelium
    Gut TubeHindgutHindgut Endoderm CellsEndoderm
    Gut TubeMidgutMidgut Endoderm CellsEndoderm
    OvaryAntral FollicleSecondary OocyteFemale Gametocytes, Germ Cells
    Spinal CordSpinal Ventral ColumnsVentral Spinal Cord Progenitor CellsMotor Neurons
    Gut TubeHindgutGut Tube
    Gut TubeMidgutGut Tube
    LiverLiver LobuleLiver
    Spinal CordSpinal Ventral ColumnsSpinal Cord
    Expression: Positive    Negative     Selective marker
    Experimental details: Curated     Microarrays     In-situ hybridization

    See MELK Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for MELK

    SOURCE GeneReport for Unigene cluster: Hs.184339

    UniProtKB/Swiss-Prot: MELK_HUMAN, Q14680
    Tissue specificity: Expressed in placenta, kidney, thymus, testis, ovary and intestine

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    Orthologs
    for MELK gene

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section
    This gene was present in the common ancestor of eukaryotes.

    Orthologs for MELK gene from 8/26 species (see all 26)    About this table
    Organism Taxonomic
    classification    		 Gene Description Human
    Similarity    		 Orthology
    Type    		 Details
    chicken
    (Gallus gallus)    		 Aves MELK1 maternal embryonic leucine zipper kinase 67.49(n)
    62.6(a)    		   428391  NM_001031509.1  NP_001026680.1 
    lizard
    (Anolis carolinensis)    		 Reptilia MELK6
    		 --
    		 65(a)
    		 1 ↔ 1
    		 GL343201.1(2880687-2907706)
    African clawed frog
    (Xenopus laevis)    		 Amphibia LOC3979272 p69Eg3 76.18(n)    Z17205.2 
    zebrafish
    (Danio rerio)    		 Actinopterygii wufb71b012 Danio rerio mRNA similar to maternal embryonic leucine more 76.33(n)    AB108827.1 
    honey bee
    (Apis mellifera)    		 Insecta --
    		 --
    		 43(a)
    		 1 ↔ 1
    		 Group4.19(128700-132669)
    worm
    (Caenorhabditis elegans)    		 Secernentea pig-11 Protein PIG-1 48.79(n)
    41.86(a)    		   176877  NM_001028249.2  NP_001023420.2 
    thale cress
    (Arabidopsis thaliana)    		 eudicotyledons CIPK151 CBL-interacting serine/threonine-protein kinase 15 53.07(n)
    45.49(a)    		   830556  NM_001036743.1  NP_001031820.1 
    rice
    (Oryza sativa)    		 Liliopsida Os07g06786001 hypothetical protein 54.77(n)
    45.88(a)    		   4344287  NM_001067171.1  NP_001060636.1 


    ENSEMBL Gene Tree for MELK (if available)
    TreeFam Gene Tree for MELK (if available) 

    Paralogs
    for MELK gene

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for MELK gene
    TSSK1B2  PRKAA22  HUNK2  TSSK62  TSSK32  PRKAA12  TSSK22  TSSK42  
    STK112  
    18/81 SIMAP similar genes for MELK using alignment to 3 protein entries:     MELK_HUMAN (see all proteins) (see all similar genes):
    MELKV4    BRSK2    PRKAA1    MARK4    PRKAA2    CAMK1D
    HUNK    KIAA0999    RPS6KA5    MARK2    TRIB1    PHKG1
    NEK9    CAMK1    MARK3    TRIB2    TSSK4    BRAF

    MELK for paralogs           About GeneDecksing



    Genomic Variants
    for MELK gene

    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/1720 NCBI SNPs in MELK are shown (see all 1720)     About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance    		Chr 9 posSequence#AA
    Chg    		TypeMore#Allele
    freq    		PopTotal
    sample    		More
    ----------
    rs1398243571,2
    		--36570894(+) CTTTGA/CGGGGT 10 -- us2k10--------
    rs1431285781,2
    		--36571039(+) CTTACA/GGATGA 10 -- us2k10--------
    rs1865472461,2
    		--36571063(+) GCTTTC/TTTGAG 10 -- us2k10--------
    rs1112576711,2
    		C,--36571381(+) AAAAGA/GTTATG 10 -- us2k12Minor allele frequency- G:0.04NA 122
    rs734369431,2
    		C,F,--36571422(+) ATTAAG/TTTTAG 10 -- us2k13Minor allele frequency- T:0.13WA CSA 122
    rs1113682491,2
    		F--36571451(+) TGCATT/ATTAAG 10 -- us2k12Minor allele frequency- A:0.50CSA 4
    rs3293551,2
    		C,F,--36571473(-) AGAAAT/GAAATT 10 -- us2k1 tfbs39Minor allele frequency- G:0.25NA WA CSA EA 368
    rs1897641291,2
    		--36571498(+) TTCTCC/TCCCTT 10 -- us2k10--------
    rs1474128241,2
    		--36571512(+) TTCCT-/TTCCC 
                TTCCC     		10 -- us2k10--------
    rs1437303251,2
    		--36571528(+) TCCCT-/TCCCC 
                TCCCT     		10 -- us2k10--------

    HapMap Linkage Disequilibrium report for MELK (36572859 - 36677679 bp)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
          Database of Genomic Variants (DGV) variations for MELK: --

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    Disorders / Diseases
    for MELK gene

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section
    MELK for disorders           About GeneDecksing

    OMIM gene information: 607025    OMIM disorders: --

    UniProtKB/Swiss-Prot: MELK_HUMAN, Q14680
  • Note=Defects in MELK are associated with some cancers, such as brain or breast cancers. Expression is
    • dramatically increased in aggressive undifferentiated tumors, correlating with poor patient outcome in breast and
    brain cancers, suggesting a role in tumor-initiating cells and proliferation via its function in cell proliferation
    regulation

    9 diseases for MELK:    About MalaCards
    brain cancer    hermaphroditism    hematopoiesis    colorectal cancer
    astrocytoma    breast cancer    glioblastoma    neuronitis
    alcoholism


    Export disorders for MELK gene to outside databases

    Publications
    for MELK gene

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for MELK gene, integrated from 9 sources (see all 42):
    (articles sorted by number of sources associating them with MELK)    		    Utopia: connect your pdf to the dynamic
    world of online information

    1. Prediction of the coding sequences of unidentified human genes. V. The coding sequences of 40 new genes (KIAA0161-KIAA0200) deduced by analysis of cDNA clones from human cell line KG-1. (PubMed id 8724849)1, 2, 3 Nagase T.... Nomura N. (1996)
    2. Maternal embryonic leucine zipper kinase transcript abundance correlates with malignancy grade in human astrocytomas. (PubMed id 17960622)1, 2, 9 Marie S.K....Caballero O.L. (2008)
    3. Maternal embryonic leucine zipper kinase is a key regulator of the proliferation of malignant brain tumors, including brain tumor stem cells. (PubMed id 17722061)1, 2, 9 Nakano I....Kornblum H.I. (2008)
    4. Involvement of maternal embryonic leucine zipper kinase (MELK) in mammary carcinogenesis through interaction with Bcl-G, a pro-apoptotic member of the Bcl-2 family. (PubMed id 17280616)1, 2, 9 Lin M.L....Katagiri T. (2007)
    5. Human pEg3 kinase associates with and phosphorylates CDC25B phosphatase: a potential role for pEg3 in cell cycle regulation. (PubMed id 12400006)1, 2, 9 Davezac N....Tassan J.P. (2002)
    6. Dysregulated expression of Fau and MELK is associated with poor prognosis in breast cancer. (PubMed id 19671159)1, 2, 9 Pickard M.R....Williams G.T. (2009)
    7. Substrate specificity and activity regulation of protein kinase MELK. (PubMed id 16216881)1, 2, 9 Beullens M....Bollen M. (2005)
    8. CDC25B phosphorylated by pEg3 localizes to the centrosome and the spindle poles at mitosis. (PubMed id 15908796)1, 2, 9 Mirey G....Ducommun B. (2005)
    9. Inhibition of spliceosome assembly by the cell cycle-regulated protein kinase MELK and involvement of splicing factor NIPP1. (PubMed id 14699119)1, 2, 9 Vulsteke V.... Bollen M. (2004)
    10. Siomycin A targets brain tumor stem cells partially through a MELK- mediated pathway. (PubMed id 21558073)1, 2 Nakano I.... Kornblum H.I. (2011)

    External Searches for MELK gene

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section
     ANDOR
    Aliases
    Free Text  
      Query String
    		PubMed
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      (Note: In FireFox, select the above section and copy using Ctrl-C)

    Genome Databases showing MELK gene

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 9833 HGNC: 16870 AceView: MELK Ensembl:ENSG00000165304 euGenes: HUgn9833
    ECgene: MELK H-InvDB: MELK

    Other Databases showing MELK gene

    (According to HUGE)
    About This Section
    HUGE: KIAA0175

    Specialized Databases showing MELK gene

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for MELK Pharmacogenomics, SNPs, Pathways

    Intellectual Property
    for MELK gene

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section
    		Patent Information for MELK gene:
    Search GeneIP for patents involving MELK

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



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