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Aliases for MED8 Gene

Aliases for MED8 Gene

  • Mediator Complex Subunit 8 2 3 4
  • Activator-Recruited Cofactor 32 KDa Component 3 4
  • ARC32 3 4
  • Mediator Of RNA Polymerase II Transcription, Subunit 8 Homolog (S. Cerevisiae) 2
  • Mediator Of RNA Polymerase II Transcription Subunit MED8 3
  • Mediator Of RNA Polymerase II Transcription Subunit 8 3

External Ids for MED8 Gene

Summaries for MED8 Gene

Entrez Gene Summary for MED8 Gene

  • This gene encodes a protein component of the mediator complex, which aids in transcriptional activation through interaction with RNA polymerase II and gene-specific transcription factors. The encoded protein may also function in ubiquitin ligation and protein degradation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2013]

GeneCards Summary for MED8 Gene

MED8 (Mediator Complex Subunit 8) is a Protein Coding gene. Among its related pathways are L1CAM interactions and Metabolism. GO annotations related to this gene include RNA polymerase II transcription cofactor activity.

UniProtKB/Swiss-Prot for MED8 Gene

  • Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May play a role as a target recruitment subunit in E3 ubiquitin-protein ligase complexes and thus in ubiquitination and subsequent proteasomal degradation of target proteins

Gene Wiki entry for MED8 Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for MED8 Gene

Genomics for MED8 Gene

Genomic Location for MED8 Gene

Start:
43,383,908 bp from pter
End:
43,389,812 bp from pter
Size:
5,905 bases
Orientation:
Minus strand

Genomic View for MED8 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for MED8 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for MED8 Gene

Regulatory Elements for MED8 Gene

Proteins for MED8 Gene

  • Protein details for MED8 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q96G25-MED8_HUMAN
    Recommended name:
    Mediator of RNA polymerase II transcription subunit 8
    Protein Accession:
    Q96G25
    Secondary Accessions:
    • A9IZ91
    • A9IZ92
    • Q5JUY8
    • Q96FQ4

    Protein attributes for MED8 Gene

    Size:
    268 amino acids
    Molecular mass:
    29080 Da
    Quaternary structure:
    • Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. May be part of a multisubunit E3 ubiquitin-protein ligase complex with the elongin BC complex (TCEB1 and TCEB2), CUL2 and RBX1.
    SequenceCaution:
    • Sequence=BG722466; Type=Frameshift; Positions=258; Evidence={ECO:0000305};

    Alternative splice isoforms for MED8 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for MED8 Gene

Proteomics data for MED8 Gene at MOPED

Post-translational modifications for MED8 Gene

  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Other Protein References for MED8 Gene

ENSEMBL proteins:
Reactome Protein details:
REFSEQ proteins:

No data available for DME Specific Peptides for MED8 Gene

Domains for MED8 Gene

Protein Domains for MED8 Gene

InterPro:
ProtoNet:

UniProtKB/Swiss-Prot:

MED8_HUMAN
Domain:
  • The elongin BC complex binding domain is also known as BC-box with the consensus [APST]-L-x(3)-C-x(3)-[AILV]:
    • Q96G25
Family:
  • Belongs to the Mediator complex subunit 8 family.:
    • Q96G25
genes like me logo Genes that share domains with MED8: view

No data available for Gene Families for MED8 Gene

Function for MED8 Gene

Molecular function for MED8 Gene

UniProtKB/Swiss-Prot Function: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May play a role as a target recruitment subunit in E3 ubiquitin-protein ligase complexes and thus in ubiquitination and subsequent proteasomal degradation of target proteins

Gene Ontology (GO) - Molecular Function for MED8 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0001104 RNA polymerase II transcription cofactor activity IEA --
GO:0005515 protein binding IPI 12149480
genes like me logo Genes that share ontologies with MED8: view

Phenotypes for MED8 Gene

GenomeRNAi human phenotypes for MED8:
genes like me logo Genes that share phenotypes with MED8: view

miRNA for MED8 Gene

No data available for Enzyme Numbers (IUBMB) , Animal Models , Transcription Factor Targeting and HOMER Transcription for MED8 Gene

Localization for MED8 Gene

Subcellular locations from UniProtKB/Swiss-Prot for MED8 Gene

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for MED8 Gene COMPARTMENTS Subcellular localization image for MED8 gene
Compartment Confidence
nucleus 5
cytosol 2
golgi apparatus 1

Gene Ontology (GO) - Cellular Components for MED8 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005654 nucleoplasm TAS --
GO:0016592 mediator complex IDA 14638676
genes like me logo Genes that share ontologies with MED8: view

Pathways for MED8 Gene

genes like me logo Genes that share pathways with MED8: view

UniProtKB/Swiss-Prot Q96G25-MED8_HUMAN

  • Pathway: Protein modification; protein ubiquitination

Gene Ontology (GO) - Biological Process for MED8 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006357 regulation of transcription from RNA polymerase II promoter IEA --
GO:0006367 transcription initiation from RNA polymerase II promoter TAS --
GO:0010467 gene expression TAS --
GO:0016567 protein ubiquitination IEA --
genes like me logo Genes that share ontologies with MED8: view

Transcripts for MED8 Gene

Unigene Clusters for MED8 Gene

Mediator complex subunit 8:
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for MED8 Gene

ExUns: 1 ^ 2a · 2b ^ 3a · 3b · 3c ^ 4 ^ 5 ^ 6a · 6b · 6c ^ 7a · 7b · 7c ^ 8
SP1: - -
SP2: -
SP3: -
SP4:
SP5: - -
SP6:

Relevant External Links for MED8 Gene

GeneLoc Exon Structure for
MED8
ECgene alternative splicing isoforms for
MED8

Expression for MED8 Gene

mRNA expression in normal human tissues for MED8 Gene

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, MOPED, and MaxQB for MED8 Gene

SOURCE GeneReport for Unigene cluster for MED8 Gene Hs.301756

genes like me logo Genes that share expressions with MED8: view

Orthologs for MED8 Gene

This gene was present in the common ancestor of animals.

Orthologs for MED8 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia MED8 36
  • 99.88 (n)
  • 99.63 (a)
MED8 37
  • 99 (a)
OneToOne
cow
(Bos Taurus)
Mammalia MED8 37
  • 96 (a)
OneToOne
dog
(Canis familiaris)
Mammalia MED8 36
  • 91.54 (n)
  • 97.01 (a)
MED8 37
  • 67 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Med8 36
  • 90.42 (n)
  • 96.64 (a)
Med8 16
Med8 37
  • 97 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia MED8 37
  • 91 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia MED8 37
  • 89 (a)
OneToOne
chicken
(Gallus gallus)
Aves MED8 36
  • 77.99 (n)
  • 88.81 (a)
MED8 37
  • 89 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia MED8 37
  • 76 (a)
OneToOne
African clawed frog
(Xenopus laevis)
Amphibia Xl.8572 36
tropical clawed frog
(Silurana tropicalis)
Amphibia med8 36
  • 70.29 (n)
  • 78.28 (a)
Str.685 36
rainbow trout
(Oncorhynchus mykiss)
Actinopterygii Omy.6726 36
zebrafish
(Danio rerio)
Actinopterygii Dr.15496 36
med8 36
  • 66.41 (n)
  • 68.82 (a)
med8 37
  • 72 (a)
OneToMany
MED8 (2 of 2) 37
  • 62 (a)
OneToMany
African malaria mosquito
(Anopheles gambiae)
Insecta MED8_ANOGA 36
  • 52.79 (n)
  • 47.76 (a)
fruit fly
(Drosophila melanogaster)
Insecta MED8 36
  • 54.12 (n)
  • 48.99 (a)
MED8 37
  • 46 (a)
OneToOne
worm
(Caenorhabditis elegans)
Secernentea mdt-8 37
  • 21 (a)
OneToOne
sea squirt
(Ciona savignyi)
Ascidiacea CSA.2247 37
  • 41 (a)
OneToOne
Species with no ortholog for MED8:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rat (Rattus norvegicus)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for MED8 Gene

ENSEMBL:
Gene Tree for MED8 (if available)
TreeFam:
Gene Tree for MED8 (if available)

Paralogs for MED8 Gene

No data available for Paralogs for MED8 Gene

Variants for MED8 Gene

Sequence variations from dbSNP and Humsavar for MED8 Gene

SNP ID Clin Chr 01 pos Sequence Context AA Info Type MAF
rs11172 -- 43,384,802(+) ATTTT(A/G)ATCCT utr-variant-3-prime, intron-variant
rs710250 -- 43,383,497(+) GAGCC(A/G)GAACA downstream-variant-500B
rs1065676 -- 43,386,615(-) CAGCA(A/C)AGAGG reference, missense
rs5773807 -- 43,386,132(+) CTGAA(-/A)GGTCT reference, frameshift-variant
rs11541153 -- 43,386,126(-) TCAGG(C/T)AGCAG reference, synonymous-codon

Relevant External Links for MED8 Gene

HapMap Linkage Disequilibrium report
MED8

No data available for Structural Variations from Database of Genomic Variants (DGV) for MED8 Gene

Disorders for MED8 Gene

No disorders were found for MED8 Gene.

No data available for UniProtKB/Swiss-Prot for MED8 Gene

Publications for MED8 Gene

  1. Mammalian mediator subunit mMED8 is an Elongin BC-interacting protein that can assemble with Cul2 and Rbx1 to reconstitute a ubiquitin ligase. (PMID: 12149480) Brower C.S. … Conaway R.C. (Proc. Natl. Acad. Sci. U.S.A. 2002) 2 3 4
  2. Identification of mammalian Mediator subunits with similarities to yeast Mediator subunits Srb5, Srb6, Med11, and Rox3. (PMID: 12584197) Sato S. … Conaway J.W. (J. Biol. Chem. 2003) 3 4
  3. Mammalian mediator of transcriptional regulation and its possible role as an end-point of signal transduction pathways. (PMID: 9671713) Jiang Y.W. … Kornberg R.D. (Proc. Natl. Acad. Sci. U.S.A. 1998) 2 3
  4. Composite co-activator ARC mediates chromatin-directed transcriptional activation. (PMID: 10235267) Naeaer A.M. … Tjian R. (Nature 1999) 3 4
  5. A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology. (PMID: 15175163) Sato S. … Conaway R.C. (Mol. Cell 2004) 3 4

Products for MED8 Gene

  • Addgene plasmids for MED8

Sources for MED8 Gene

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