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MED8 Gene

protein-coding   GIFtS: 52
GCID: GC01M043849

Mediator Complex Subunit 8

(Previous names: mediator of RNA polymerase II transcription, subunit 8 homolog...)
  See related diseases
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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Aliases
Mediator Complex Subunit 81 2 3
Activator-Recruited Cofactor 32 KDa Component2 3
ARC322 3
Mediator Of RNA Polymerase II Transcription, Subunit 8 Homolog (S.
Cerevisiae)1
Mediator Of RNA Polymerase II Transcription Subunit 82
Mediator Of RNA Polymerase II Transcription Subunit MED82

External Ids:    HGNC: 199711   Entrez Gene: 1129502   Ensembl: ENSG000001594797   OMIM: 6079565   UniProtKB: Q96G253   

Export aliases for MED8 gene to outside databases

Previous GC identifers: GC01U990453 GC01M042850 GC01M043279 GC01M043262 GC01M043518 GC01M043622 GC01M041968


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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Entrez Gene summary for MED8 Gene:
This gene encodes a protein component of the mediator complex, which aids in transcriptional activation through
interaction with RNA polymerase II and gene-specific transcription factors. The encoded protein may also function
in ubiquitin ligation and protein degradation. Alternatively spliced transcript variants encoding multiple
isoforms have been observed for this gene. (provided by RefSeq, Jan 2013)

GeneCards Summary for MED8 Gene:
MED8 (mediator complex subunit 8) is a protein-coding gene. Diseases associated with MED8 include intrahepatic cholangiocarcinoma, and herpes simplex. GO annotations related to this gene include RNA polymerase II transcription cofactor activity.

UniProtKB/Swiss-Prot: MED8_HUMAN, Q96G25
Function: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all
RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific
regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by
direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional
preinitiation complex with RNA polymerase II and the general transcription factors. May play a role as a target
recruitment subunit in E3 ubiquitin-protein ligase complexes and thus in ubiquitination and subsequent
proteasomal degradation of target proteins

Gene Wiki entry for MED8 Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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RefSeq DNA sequence:
NC_000001.11  NC_018912.2  NT_032977.10  
Regulatory elements:
   Regulatory transcription factor binding sites in the MED8 gene promoter:
         TBP   ISGF-3   NF-1/L   NF-1   AP-4   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidMED8 promoter sequence
   Search Chromatin IP Primers for MED8

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat MED8


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 1p34.2   Ensembl cytogenetic band:  1p34.2   HGNC cytogenetic band: 1p34.1

MED8 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
MED8 gene location

GeneLoc information about chromosome 1         GeneLoc Exon Structure

GeneLoc location for GC01M043849:  view genomic region     (about GC identifiers)

Start:
43,849,588 bp from pter      End:
43,855,483 bp from pter
Size:
5,896 bases      Orientation:
minus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Cloud-Clone Corp.,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Cloud-Clone Corp., Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, and/or Cloud-Clone Corp.)
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UniProtKB/Swiss-Prot: MED8_HUMAN, Q96G25 (See protein sequence)
Recommended Name: Mediator of RNA polymerase II transcription subunit 8  
Size: 268 amino acids; 29080 Da
Subunit: Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11,
MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26,
MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct
module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this
module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or
more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. May be part of a
multisubunit E3 ubiquitin-protein ligase complex with the elongin BC complex (TCEB1 and TCEB2), CUL2 and RBX1
Sequence caution: Sequence=BG722466; Type=Frameshift; Positions=258;
Secondary accessions: A9IZ91 A9IZ92 Q5JUY8 Q96FQ4
Alternative splicing: 3 isoforms:  Q96G25-1   Q96G25-2   Q96G25-3   (No experimental confirmation available)

Explore the universe of human proteins at neXtProt for MED8: NX_Q96G25

Explore proteomics data for MED8 at MOPED

Post-translational modifications: 

  • Modification sites at neXtProt
  • Modification sites at PhosphoSitePlus

  • See MED8 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins (3 alternative transcripts): 
    NP_001001653.1  NP_443109.2  NP_963836.2  

    ENSEMBL proteins: 
     ENSP00000290663   ENSP00000361535   ENSP00000361533  
    Reactome Protein details: Q96G25

    MED8 Human Recombinant Protein Products:

    Browse Purified and Recombinant Proteins at EMD Millipore
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    OriGene Purified Protein for MED8
    OriGene Protein Over-expression Lysate for MED8
    OriGene MassSpec for MED8
    OriGene Custom Protein Services for MED8
    GenScript Custom Purified and Recombinant Proteins Services for MED8
    Novus Biologicals MED8 Proteins
    Novus Biologicals MED8 Lysates
    Browse Sino Biological Recombinant Proteins
    Browse Sino Biological Cell Lysates
    Browse ProSpec Recombinant Proteins
    Cloud-Clone Corp. Proteins for MED8

    MED8 Antibody Products:

    Browse EMD Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Browse R&D Systems for Antibodies
    OriGene Antibodies for MED8
    OriGene Custom Antibody Services for MED8
    Novus Biologicals MED8 Antibodies
    Abcam antibodies for MED8
    Cloud-Clone Corp. Antibodies for MED8
    ThermoFisher Antibodies for MED8
    LSBio Antibodies in human, mouse, rat for MED8

    MED8 Assay Products:

    Browse Kits and Assays available from EMD Millipore
    OriGene Custom Assay Services for MED8
    Browse R&D Systems for biochemical assays
    GenScript Custom Assay Services for MED8
    Browse Enzo Life Sciences for kits & assays
    Cloud-Clone Corp. ELISAs for MED8
    Cloud-Clone Corp. CLIAs for MED8


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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    1 InterPro protein domain:
     IPR019364 Mediatior_Med8_fun/met

    Graphical View of Domain Structure for InterPro Entry Q96G25

    ProtoNet protein and cluster: Q96G25

    UniProtKB/Swiss-Prot: MED8_HUMAN, Q96G25
    Domain: The elongin BC complex binding domain is also known as BC-box with the consensus
    [APST]-L-x(3)-C-x(3)-[AILV]
    Similarity: Belongs to the Mediator complex subunit 8 family


    MED8 for domains           About GeneDecksing


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Molecular Function:

         UniProtKB/Swiss-Prot Summary: MED8_HUMAN, Q96G25
    Function: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all
    RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific
    regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by
    direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional
    preinitiation complex with RNA polymerase II and the general transcription factors. May play a role as a target
    recruitment subunit in E3 ubiquitin-protein ligase complexes and thus in ubiquitination and subsequent
    proteasomal degradation of target proteins

         Gene Ontology (GO): 2 molecular function terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0001104RNA polymerase II transcription cofactor activity IEA--
    GO:0005515protein binding IPI12149480
         
    MED8 for ontologies           About GeneDecksing


    Phenotypes:
         1 GenomeRNAi human phenotype for MED8:
     Increased gamma-H2AX phosphory 

    Animal Models:
       inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for MED8
       inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for MED8

       genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for MED8
       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for MED8

    miRNA
    Products:
        
    miRTarBase miRNAs that target MED8:
    hsa-mir-186-5p (MIRT045059), hsa-mir-615-3p (MIRT040043), hsa-mir-98-5p (MIRT027422), hsa-mir-375 (MIRT019689)

    Block miRNA regulation of human, mouse, rat MED8 using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate MED8 (see all 20):
    hsa-miR-345 hsa-miR-181c hsa-let-7d hsa-miR-202 hsa-let-7c hsa-miR-500a hsa-let-7g hsa-let-7a
    SwitchGear 3'UTR luciferase reporter plasmidMED8 3' UTR sequence
    Inhib. RNA
    Products:
        
    OriGene RNAi products in human, mouse, rat for MED8
    Predesigned siRNA for gene silencing in human, mouse, rat MED8

    Gene Editing
    Products:
    DNA2.0 Custom Protein Engineering Service for MED8

    Clone
    Products:
         
    OriGene clones in human, mouse for MED8 (see all 21)
    OriGene ORF clones in mouse, rat for MED8
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector (see all 2): MED8 (NM_052877)
    Browse Sino Biological Human cDNA Clones
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for MED8
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat MED8

    Cell Line
    Products:
         
    GenScript Custom overexpressing Cell Line Services for MED8
    Browse ESI BIO Cell Lines and PureStem Progenitors for MED8 
    In Situ Assay
    Products:
       

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for MED8


    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Subcellular locations from UniProtKB/Swiss-Prot
    MED8_HUMAN, Q96G25: Nucleus (Probable)
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    nucleus5
    cytosol2
    golgi apparatus1

    Gene Ontology (GO): 2 cellular component terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005654nucleoplasm TAS--
    GO:0016592mediator complex IDA14638676

    MED8 for ontologies           About GeneDecksing


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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    SuperPaths for MED8 About    
    See pathways by source

    SuperPathContained pathways About
    1Fatty acid, triacylglycerol, and ketone body metabolism
    Fatty acid, triacylglycerol, and ketone body metabolism0.65
    PPARA Activates Gene Expression0.63
    Regulation of Lipid Metabolism by Peroxisome proliferator-activated receptor alpha (PPARalpha)0.65
    Transcriptional Regulation of White Adipocyte Differentiation0.38
    2Gene Expression
    Generic Transcription Pathway0.40
    Gene Expression0.40
    3Metabolism
    Metabolism0.38
    Metabolism of lipids and lipoproteins0.37
    4L1CAM interactions
    Developmental Biology0.63
    5Influenza A
    Herpes simplex infection0.33

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways


    3 Reactome Pathways for MED8
        Transcriptional regulation of white adipocyte differentiation
    Generic Transcription Pathway
    PPARA activates gene expression


    1 Kegg Pathway  (Kegg details for MED8):
        Herpes simplex infection

    UniProtKB/Swiss-Prot: MED8_HUMAN, Q96G25
    Pathway: Protein modification; protein ubiquitination


    MED8 for pathways           About GeneDecksing

        Custom Pathway & Disease-focused RT2 Profiler PCR Arrays for MED8
    Interactions:

        GeneGlobe Interaction Network for MED8

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    Selected Interacting proteins for MED8 (Q96G251, 2, 3 ENSP000002906634) via UniProtKB, MINT, STRING, and/or I2D (see all 128)

    InteractantInteraction Details
    GeneCardExternal ID(s)
    PEX14O753812, 3, ENSP000003490164MINT-63204 I2D: score=5 STRING: ENSP00000349016
    TCEB2Q153701, 3, ENSP000002623064EBI-394405,EBI-301238 I2D: score=2 STRING: ENSP00000262306
    HSPB3Q129882, 3MINT-64108 I2D: score=5 
    SNTA1Q134242, 3MINT-64135 I2D: score=4 
    TCEB1Q153691, 3, ENSP000002848114EBI-394405,EBI-301231 I2D: score=4 STRING: ENSP00000284811
    About this table

    Gene Ontology (GO): 4 biological process terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006357regulation of transcription from RNA polymerase II promoter ----
    GO:0006367transcription initiation from RNA polymerase II promoter TAS--
    GO:0010467gene expression TAS--
    GO:0016567protein ubiquitination IEA--

    MED8 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Browse Tocris compounds for MED8



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    Conferences by KenesGroup, exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, and/or QIAGEN )
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    REFSEQ mRNAs for MED8 gene (3 alternative transcripts): 
    NM_001001653.2  NM_052877.4  NM_201542.4  

    Unigene Cluster for MED8:

    Mediator complex subunit 8
    Hs.301756  [show with all ESTs]
    Unigene Representative Sequence: NM_001001653
    5 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000290663(uc001cje.1) ENST00000372457(uc001cjg.4) ENST00000372455(uc001cjf.4)
    ENST00000460803 ENST00000473560
    miRNA
    Products:
         
    Block miRNA regulation of human, mouse, rat MED8 using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate MED8 (see all 20):
    hsa-miR-345 hsa-miR-181c hsa-let-7d hsa-miR-202 hsa-let-7c hsa-miR-500a hsa-let-7g hsa-let-7a
    SwitchGear 3'UTR luciferase reporter plasmidMED8 3' UTR sequence
    Inhib. RNA
    Products:
         
    OriGene RNAi products in human, mouse, rat for MED8
    Predesigned siRNA for gene silencing in human, mouse, rat MED8
    Clone
    Products:
         
    OriGene clones in human, mouse for MED8 (see all 21)
    OriGene ORF clones in mouse, rat for MED8
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector (see all 2): MED8 (NM_052877)
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for MED8
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat MED8
    Primer
    Products:
        
    OriGene qPCR primer pairs and template standards for MED8
    OriGene qSTAR qPCR primer pairs in human, mouse for MED8
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat MED8
      QuantiTect SYBR Green Assays in human, mouse, rat MED8
      QuantiFast Probe-based Assays in human, mouse, rat MED8

    Additional mRNA sequence: 

    AF521562.1 BC010019.2 BC010543.1 

    16 DOTS entries:

    DT.92470414  DT.102837558  DT.100707551  DT.97848207  DT.100028374  DT.448349  DT.100886652  DT.100728317 
    DT.92470444  DT.91747218  DT.92470393  DT.121444985  DT.95327446  DT.75104753  DT.92020732  DT.99972132 

    Selected AceView cDNA sequences (see all 728):

    CA311642 CA308147 BQ687894 CR620696 CR623988 BU734663 BM706150 BX393888 
    CR592943 AL537185 CB148892 CA426833 AA535341 CB113308 BG108027 BG054823 
    CR600285 AV698544 BQ011155 AV698542 BI762591 CR598270 BM764500 AI205948 

    GeneLoc Exon Structure

    Selected Alternative Splicing Database (ASD) splice patterns (SP) for MED8 (see all 6)    About this scheme

    ExUns: 1 ^ 2a · 2b ^ 3a · 3b · 3c ^ 4 ^ 5 ^ 6a · 6b · 6c ^ 7a · 7b · 7c ^ 8
    SP1:                                                                          -     -         
    SP2:        -                                                                                 
    SP3:                    -                                                                     
    SP4:                                                                                          
    SP5:                    -     -                                                               


    ECgene alternative splicing isoforms for MED8

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
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    MED8 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: GGCAGGGGTT
    MED8 Expression
    About this image

    MED8 Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    MED8 Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.301756
        Custom PCR Arrays for MED8
    Primer
    Products:
    OriGene qPCR primer pairs and template standards for MED8
    OriGene qSTAR qPCR primer pairs in human, mouse for MED8
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat MED8
    QuantiTect SYBR Green Assays in human, mouse, rat MED8
    QuantiFast Probe-based Assays in human, mouse, rat MED8
    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for MED8

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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    This gene was present in the common ancestor of animals.

    Orthologs for MED8 gene from Selected species (see all 16)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Med81 , 5 mediator of RNA polymerase II transcription, subunit more5
    mediator complex subunit 81
    90.42(n)1
    96.64(a)1
      4 (54.61 cM)5
    805091  NM_020000.21  NP_064384.21 
     1184093375 
    chicken
    (Gallus gallus)
    Aves MED81 mediator complex subunit 8 77.99(n)
    88.81(a)
      424565  NM_001277568.1  NP_001264497.1 
    lizard
    (Anolis carolinensis)
    Reptilia MED86
    mediator complex subunit 8
    76(a)
    1 ↔ 1
    4(107350081-107363119)
    African clawed frog
    (Xenopus laevis)
    Amphibia Xl.85722 Xenopus laevis transcribed sequence with weak similarity more 73.84(n)    BU906676.1 
    zebrafish
    (Danio rerio)
    Actinopterygii Dr.154962 Transcribed sequence with weak similarity to protein more 75.38(n)    BI891271.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta MED81 Mediator complex subunit 8 54.12(n)
    48.99(a)
      37305  NM_153772.1  NP_722456.1 
    worm
    (Caenorhabditis elegans)
    Secernentea mdt-86
    Protein MDT-8, isoform a (mdt-8) mRNA, complete cd...
    21(a)
    1 ↔ 1
    II(11527176-11533814) WBGene00007013


    ENSEMBL Gene Tree for MED8 (if available)
    TreeFam Gene Tree for MED8 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
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      --

    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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    Selected SNPs for MED8 (see all 106)    About this table    
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 1 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1921029671,2
    --43849091(+) TACCCA/TATATG 3 -- ds50010--------
    rs1830691111,2
    C--43849134(+) AAGAGA/GTGAGA 3 -- ds50010--------
    rs1874027151,2
    --43849135(+) AGAGGG/TGAGAA 3 -- ds50010--------
    rs7102501,2
    C,F,H--43849168(+) GAGCCG/AGAACA 3 -- ds500111Minor allele frequency- A:0.02MN NS EA NA WA 1368
    rs1918524101,2
    --43849427(+) GCAGGA/GGGGAA 3 -- ds50010--------
    rs1501692811,2
    --43849486(+) TATGGA/CGAACA 3 -- ds50010--------
    rs1467418401,2
    C--43849632(+) ACTTCC/TGCTGA 3 -- ut310--------
    rs1842745241,2
    --43849812(+) AAGTCA/GTTTTC 3 -- ut310--------
    rs1138757511,2
    C,F--43849843(+) AAGTCG/AAGAAA 3 -- ut311Minor allele frequency- A:0.50NA 2
    rs1501816171,2
    C--43849891(+) AGTGT-/GAAGTG 3 -- ut310--------

    HapMap Linkage Disequilibrium report for MED8 (43849588 - 43855483 bp)

    Structural Variations
          Database of Genomic Variants (DGV) variations for MED8: --
    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing MED8
    DNA2.0 Custom Variant and Variant Library Synthesis for MED8

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Conferences by KenesGroup, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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    OMIM gene information: 607956    OMIM disorders: --

    7 diseases for MED8:    
    About MalaCards
    intrahepatic cholangiocarcinoma    herpes simplex    cholangiocarcinoma    hepatocellular carcinoma
    malaria    hypoxia    melanoma


    MED8 for disorders           About GeneDecksing


    Export disorders for MED8 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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    PubMed articles for MED8 gene, integrated from 10 sources (see all 34):
    (articles sorted by number of sources associating them with MED8)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Mammalian mediator subunit mMED8 is an Elongin BC-interacting protein that can assemble with Cul2 and Rbx1 to reconstitute a ubiquitin ligase. (PubMed id 12149480)1, 2, 3 Brower C.S.... Conaway R.C. (Proc. Natl. Acad. Sci. U.S.A. 2002)
    2. MED1/TRAP220 exists predominantly in a TRAP/Mediator subpopulation enriched in RNA polymerase II and is required for ER-mediated transcription. (PubMed id 15989967)1, 2 Zhang X....Roeder R.G. (Mol. Cell 2005)
    3. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S.... Malek J. (Genome Res. 2004)
    4. A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology. (PubMed id 15175163)1, 2 Sato S.... Conaway R.C. (Mol. Cell 2004)
    5. Identification of mammalian Mediator subunits with similarities to yeast Mediator subunits Srb5, Srb6, Med11, and Rox3. (PubMed id 12584197)1, 2 Sato S....Conaway J.W. (J. Biol. Chem. 2003)
    6. Composite co-activator ARC mediates chromatin-directed transcriptional activation. (PubMed id 10235267)1, 2 Naeaer A.M....Tjian R. (Nature 1999)
    7. Mammalian mediator of transcriptional regulation and its possible role as an end-point of signal transduction pathways. (PubMed id 9671713)1, 3 Jiang Y.W.... Kornberg R.D. (Proc. Natl. Acad. Sci. U.S.A. 1998)
    8. Identification of the hypoxia-inducible factor 2I+ nuclear interactome in melanoma cells reveals master proteins involved in melanoma development. (PubMed id 23275444)1 Steunou A.L....Nieto L. (amp 2013)
    9. The protein interaction landscape of the human CMGC kinase group. (PubMed id 23602568)1 Varjosalo M....Gstaiger M. (Cell Rep 2013)
    10. The SCF-Fbw7 ubiquitin ligase degrades MED13 and MED13L and regulates CDK8 module association with Mediator. (PubMed id 23322298)1 Davis M.A....Clurman B.E. (amp 2013)

    (in PubMed, OMIM, and NCBI Bookshelf)
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     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Entrez Gene: 112950 HGNC: 19971 AceView: ELOVL1andMED8 Ensembl:ENSG00000159479 euGenes: HUgn112950
    ECgene: MED8 Kegg: 112950 H-InvDB: MED8

    (According to HUGE)
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      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
    About This Section

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    NameDescription
    PharmGKB entry for MED8 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section

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    Patent Information for MED8 gene:
    Search GeneIP for patents involving MED8

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript, and ESI BIO, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
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    GeneCards Homepage - Last full update: 7 May 2014 - Incrementals: 9 May 2014 , 2 Jun 2014 , 26 Jun 2014 , 30 Jun 2014

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