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Aliases for MED8 Gene

Aliases for MED8 Gene

  • Mediator Complex Subunit 8 2 3 4 5
  • Activator-Recruited Cofactor 32 KDa Component 3 4
  • ARC32 3 4
  • Mediator Of RNA Polymerase II Transcription, Subunit 8 Homolog (S. Cerevisiae) 2
  • Mediator Of RNA Polymerase II Transcription Subunit MED8 3

External Ids for MED8 Gene

Previous GeneCards Identifiers for MED8 Gene

  • GC01U990453
  • GC01M042850
  • GC01M043279
  • GC01M043262
  • GC01M043518
  • GC01M043622
  • GC01M043849
  • GC01M041968

Summaries for MED8 Gene

Entrez Gene Summary for MED8 Gene

  • This gene encodes a protein component of the mediator complex, which aids in transcriptional activation through interaction with RNA polymerase II and gene-specific transcription factors. The encoded protein may also function in ubiquitin ligation and protein degradation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2013]

GeneCards Summary for MED8 Gene

MED8 (Mediator Complex Subunit 8) is a Protein Coding gene. Among its related pathways are Gene Expression and Developmental Biology. GO annotations related to this gene include RNA polymerase II transcription cofactor activity.

UniProtKB/Swiss-Prot for MED8 Gene

  • Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May play a role as a target recruitment subunit in E3 ubiquitin-protein ligase complexes and thus in ubiquitination and subsequent proteasomal degradation of target proteins.

Gene Wiki entry for MED8 Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for MED8 Gene

Genomics for MED8 Gene

Regulatory Elements for MED8 Gene

Enhancers for MED8 Gene
GeneHancer Identifier Score Enhancer Sources TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Other Gene Targets for Enhancer

Enhancers around MED8 on UCSC Golden Path with GeneCards custom track

Promoters for MED8 Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters

ENSRs around MED8 on UCSC Golden Path with GeneCards custom track

Genomic Location for MED8 Gene

Chromosome:
1
Start:
43,383,908 bp from pter
End:
43,389,812 bp from pter
Size:
5,905 bases
Orientation:
Minus strand

Genomic View for MED8 Gene

Genes around MED8 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
MED8 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for MED8 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for MED8 Gene

Proteins for MED8 Gene

  • Protein details for MED8 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q96G25-MED8_HUMAN
    Recommended name:
    Mediator of RNA polymerase II transcription subunit 8
    Protein Accession:
    Q96G25
    Secondary Accessions:
    • A9IZ91
    • A9IZ92
    • Q5JUY8
    • Q96FQ4

    Protein attributes for MED8 Gene

    Size:
    268 amino acids
    Molecular mass:
    29080 Da
    Quaternary structure:
    • Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. May be part of a multisubunit E3 ubiquitin-protein ligase complex with the elongin BC complex (TCEB1 and TCEB2), CUL2 and RBX1.
    SequenceCaution:
    • Sequence=BG722466; Type=Frameshift; Positions=258; Evidence={ECO:0000305};

    Alternative splice isoforms for MED8 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for MED8 Gene

Proteomics data for MED8 Gene at MOPED

Post-translational modifications for MED8 Gene

  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Other Protein References for MED8 Gene

No data available for DME Specific Peptides for MED8 Gene

Domains & Families for MED8 Gene

Gene Families for MED8 Gene

Protein Domains for MED8 Gene

InterPro:
ProtoNet:

Suggested Antigen Peptide Sequences for MED8 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

Q96G25

UniProtKB/Swiss-Prot:

MED8_HUMAN :
  • The elongin BC complex binding domain is also known as BC-box with the consensus [APST]-L-x(3)-C-x(3)-[AILV].
  • Belongs to the Mediator complex subunit 8 family.
Domain:
  • The elongin BC complex binding domain is also known as BC-box with the consensus [APST]-L-x(3)-C-x(3)-[AILV].
Family:
  • Belongs to the Mediator complex subunit 8 family.
genes like me logo Genes that share domains with MED8: view

Function for MED8 Gene

Molecular function for MED8 Gene

UniProtKB/Swiss-Prot Function:
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May play a role as a target recruitment subunit in E3 ubiquitin-protein ligase complexes and thus in ubiquitination and subsequent proteasomal degradation of target proteins.

Gene Ontology (GO) - Molecular Function for MED8 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0001104 RNA polymerase II transcription cofactor activity IEA --
genes like me logo Genes that share ontologies with MED8: view
genes like me logo Genes that share phenotypes with MED8: view

Animal Model Products

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for MED8 Gene

Localization for MED8 Gene

Subcellular locations from UniProtKB/Swiss-Prot for MED8 Gene

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for MED8 Gene COMPARTMENTS Subcellular localization image for MED8 gene
Compartment Confidence
nucleus 5
cytosol 3
golgi apparatus 2

No data available for Gene Ontology (GO) - Cellular Components for MED8 Gene

Pathways & Interactions for MED8 Gene

genes like me logo Genes that share pathways with MED8: view

Pathways by source for MED8 Gene

UniProtKB/Swiss-Prot Q96G25-MED8_HUMAN

  • Pathway: Protein modification; protein ubiquitination.

Gene Ontology (GO) - Biological Process for MED8 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006357 regulation of transcription from RNA polymerase II promoter IEA --
GO:0006367 transcription initiation from RNA polymerase II promoter TAS --
genes like me logo Genes that share ontologies with MED8: view

No data available for SIGNOR curated interactions for MED8 Gene

Drugs & Compounds for MED8 Gene

No Compound Related Data Available

Transcripts for MED8 Gene

Unigene Clusters for MED8 Gene

Mediator complex subunit 8:
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for MED8 Gene

ExUns: 1 ^ 2a · 2b ^ 3a · 3b · 3c ^ 4 ^ 5 ^ 6a · 6b · 6c ^ 7a · 7b · 7c ^ 8
SP1: - -
SP2: -
SP3: -
SP4:
SP5: - -
SP6:

Relevant External Links for MED8 Gene

GeneLoc Exon Structure for
MED8
ECgene alternative splicing isoforms for
MED8

Expression for MED8 Gene

mRNA expression in normal human tissues for MED8 Gene

Protein differential expression in normal tissues from HIPED for MED8 Gene

This gene is overexpressed in Breast (40.1) and Peripheral blood mononuclear cells (15.1).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MOPED, and MaxQB for MED8 Gene



SOURCE GeneReport for Unigene cluster for MED8 Gene Hs.301756

genes like me logo Genes that share expression patterns with MED8: view

Protein tissue co-expression partners for MED8 Gene

Primer Products

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , mRNA differential expression in normal tissues and mRNA Expression by UniProt/SwissProt for MED8 Gene

Orthologs for MED8 Gene

This gene was present in the common ancestor of animals.

Orthologs for MED8 Gene

Organism Taxonomy Gene Similarity Type Details
dog
(Canis familiaris)
Mammalia MED8 35
  • 91.54 (n)
  • 97.01 (a)
MED8 36
  • 67 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Med8 35
  • 90.42 (n)
  • 96.64 (a)
Med8 16
Med8 36
  • 97 (a)
OneToOne
chimpanzee
(Pan troglodytes)
Mammalia MED8 35
  • 99.88 (n)
  • 99.63 (a)
MED8 36
  • 99 (a)
OneToOne
cow
(Bos Taurus)
Mammalia MED8 36
  • 96 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia MED8 36
  • 91 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia MED8 36
  • 89 (a)
OneToOne
chicken
(Gallus gallus)
Aves MED8 35
  • 77.99 (n)
  • 88.81 (a)
MED8 36
  • 89 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia MED8 36
  • 76 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia med8 35
  • 70.29 (n)
  • 78.28 (a)
Str.685 35
African clawed frog
(Xenopus laevis)
Amphibia Xl.8572 35
zebrafish
(Danio rerio)
Actinopterygii Dr.15496 35
med8 35
  • 66.41 (n)
  • 68.82 (a)
med8 36
  • 72 (a)
OneToMany
MED8 (2 of 2) 36
  • 62 (a)
OneToMany
rainbow trout
(Oncorhynchus mykiss)
Actinopterygii Omy.6726 35
African malaria mosquito
(Anopheles gambiae)
Insecta MED8_ANOGA 35
  • 52.79 (n)
  • 47.76 (a)
fruit fly
(Drosophila melanogaster)
Insecta MED8 35
  • 54.12 (n)
  • 48.99 (a)
MED8 36
  • 46 (a)
OneToOne
worm
(Caenorhabditis elegans)
Secernentea mdt-8 36
  • 21 (a)
OneToOne
sea squirt
(Ciona savignyi)
Ascidiacea CSA.2247 36
  • 41 (a)
OneToOne
Species with no ortholog for MED8:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rat (Rattus norvegicus)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for MED8 Gene

ENSEMBL:
Gene Tree for MED8 (if available)
TreeFam:
Gene Tree for MED8 (if available)

Paralogs for MED8 Gene

No data available for Paralogs for MED8 Gene

Variants for MED8 Gene

Variation tolerance for MED8 Gene

Residual Variation Intolerance Score: 52.6% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 0.72; 15.29% of all genes are more intolerant (likely to be disease-causing)

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot , Sequence variations from dbSNP and Humsavar , Structural Variations from Database of Genomic Variants (DGV) and Relevant External Links for MED8 Gene

Disorders for MED8 Gene

Relevant External Links for MED8

Atlas of Genetics and Cytogenetics in Oncology and Haematology:
MED8

No disorders were found for MED8 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for MED8 Gene

Publications for MED8 Gene

  1. Mammalian mediator subunit mMED8 is an Elongin BC-interacting protein that can assemble with Cul2 and Rbx1 to reconstitute a ubiquitin ligase. (PMID: 12149480) Brower C.S. … Conaway R.C. (Proc. Natl. Acad. Sci. U.S.A. 2002) 2 3 4 67
  2. Mammalian mediator of transcriptional regulation and its possible role as an end-point of signal transduction pathways. (PMID: 9671713) Jiang Y.W. … Kornberg R.D. (Proc. Natl. Acad. Sci. U.S.A. 1998) 2 3
  3. Proximity biotinylation and affinity purification are complementary approaches for the interactome mapping of chromatin-associated protein complexes. (PMID: 25281560) Lambert J.P. … Gingras A.C. (J Proteomics 2015) 3
  4. FBXW7 modulates cellular stress response and metastatic potential through a88HSF1 post-translational modification. (PMID: 25720964) Kourtis N. … Aifantis I. (Nat. Cell Biol. 2015) 3
  5. The BioPlex Network: A Systematic Exploration of the Human Interactome. (PMID: 26186194) Huttlin E.L. … Gygi S.P. (Cell 2015) 3

Products for MED8 Gene

Sources for MED8 Gene

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