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Aliases for MED8 Gene

Aliases for MED8 Gene

  • Mediator Complex Subunit 8 2 3 4 5
  • Activator-Recruited Cofactor 32 KDa Component 3 4
  • ARC32 3 4
  • Mediator Of RNA Polymerase II Transcription, Subunit 8 Homolog (S. Cerevisiae) 2
  • Mediator Of RNA Polymerase II Transcription Subunit MED8 3
  • Mediator Of RNA Polymerase II Transcription Subunit 8 3

External Ids for MED8 Gene

Previous GeneCards Identifiers for MED8 Gene

  • GC01U990453
  • GC01M042850
  • GC01M043279
  • GC01M043262
  • GC01M043518
  • GC01M043622
  • GC01M043849
  • GC01M041968

Summaries for MED8 Gene

Entrez Gene Summary for MED8 Gene

  • This gene encodes a protein component of the mediator complex, which aids in transcriptional activation through interaction with RNA polymerase II and gene-specific transcription factors. The encoded protein may also function in ubiquitin ligation and protein degradation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2013]

GeneCards Summary for MED8 Gene

MED8 (Mediator Complex Subunit 8) is a Protein Coding gene. Among its related pathways are Metabolism and Regulation of lipid metabolism by Peroxisome proliferator-activated receptor alpha (PPARalpha). GO annotations related to this gene include RNA polymerase II transcription cofactor activity.

UniProtKB/Swiss-Prot for MED8 Gene

  • Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May play a role as a target recruitment subunit in E3 ubiquitin-protein ligase complexes and thus in ubiquitination and subsequent proteasomal degradation of target proteins.

Gene Wiki entry for MED8 Gene

Additional gene information for MED8 Gene

No data available for CIViC summary , Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for MED8 Gene

Genomics for MED8 Gene

Regulatory Elements for MED8 Gene

Enhancers for MED8 Gene
GeneHancer Identifier Enhancer Score Enhancer Sources Gene-Enhancer Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Gene Targets for Enhancer
GH01H043357 1.3 ENCODE dbSUPER 66.7 +30.2 30166 4 PKNOX1 ARNT ARID4B SIN3A DMAP1 ZNF2 YY1 SLC30A9 FOS DEK MED8 SZT2 PPIH P3H1 HYI KDM4A-AS1 ENSG00000229431 GC01P043360 CDC20
GH01H043347 1.5 Ensembl ENCODE dbSUPER 52.7 +40.5 40473 2 ATF1 ZNF133 SIN3A FEZF1 DMAP1 ZNF2 ZBTB7B YY1 ETS1 ZNF121 MED8 LOC105378687 GC01P043344 GC01P043343
GH01H043388 1.4 ENCODE dbSUPER 55.4 +0.1 88 2 HDGF PKNOX1 ARNT ARID4B SIN3A DMAP1 ZNF2 ZNF143 FOS SP3 MED8 HYI CCDC24 CCDC30 HYI-AS1 LOC105378683 SZT2 ENSG00000229431
GH01H043371 1.6 FANTOM5 Ensembl ENCODE dbSUPER 48 +16.8 16770 3 GTF2F1 JUN BRCA1 FOSL1 ZNF316 RCOR1 FOS NFE2 TCF7L2 STAT1 MED8 MPL EBNA1BP2 CFAP57 ELOVL1 SZT2
GH01H043365 1.4 ENCODE dbSUPER 53.6 +22.6 22594 4 HDGF PKNOX1 ARNT ZFP64 ARID4B SIN3A DMAP1 ZNF2 SLC30A9 ZNF143 MED8 MPL SZT2 HYI ELOVL1 MIR6734
- Elite enhancer and/or Elite enhancer-gene association Download GeneHancer data dump

Enhancers around MED8 on UCSC Golden Path with GeneCards custom track

Promoters for MED8 Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters
ENSR00000005588 -88 1801 HDGF PKNOX1 ARNT ARID4B SIN3A DMAP1 ZNF2 ZNF143 FOS SP3

Genomic Location for MED8 Gene

Chromosome:
1
Start:
43,383,908 bp from pter
End:
43,389,812 bp from pter
Size:
5,905 bases
Orientation:
Minus strand

Genomic View for MED8 Gene

Genes around MED8 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
MED8 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for MED8 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for MED8 Gene

Proteins for MED8 Gene

  • Protein details for MED8 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q96G25-MED8_HUMAN
    Recommended name:
    Mediator of RNA polymerase II transcription subunit 8
    Protein Accession:
    Q96G25
    Secondary Accessions:
    • A9IZ91
    • A9IZ92
    • Q5JUY8
    • Q96FQ4

    Protein attributes for MED8 Gene

    Size:
    268 amino acids
    Molecular mass:
    29080 Da
    Quaternary structure:
    • Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. May be part of a multisubunit E3 ubiquitin-protein ligase complex with the elongin BC complex (ELOB and ELOC), CUL2 and RBX1.
    SequenceCaution:
    • Sequence=BG722466; Type=Frameshift; Positions=258; Evidence={ECO:0000305};

    Alternative splice isoforms for MED8 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for MED8 Gene

Post-translational modifications for MED8 Gene

  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Other Protein References for MED8 Gene

No data available for DME Specific Peptides for MED8 Gene

Domains & Families for MED8 Gene

Gene Families for MED8 Gene

HGNC:
Human Protein Atlas (HPA):
  • Plasma proteins
  • Predicted intracellular proteins

Protein Domains for MED8 Gene

InterPro:
ProtoNet:

Suggested Antigen Peptide Sequences for MED8 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

Q96G25

UniProtKB/Swiss-Prot:

MED8_HUMAN :
  • The elongin BC complex binding domain is also known as BC-box with the consensus [APST]-L-x(3)-C-x(3)-[AILV].
  • Belongs to the Mediator complex subunit 8 family.
Domain:
  • The elongin BC complex binding domain is also known as BC-box with the consensus [APST]-L-x(3)-C-x(3)-[AILV].
Family:
  • Belongs to the Mediator complex subunit 8 family.
genes like me logo Genes that share domains with MED8: view

Function for MED8 Gene

Molecular function for MED8 Gene

UniProtKB/Swiss-Prot Function:
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May play a role as a target recruitment subunit in E3 ubiquitin-protein ligase complexes and thus in ubiquitination and subsequent proteasomal degradation of target proteins.

Phenotypes From GWAS Catalog for MED8 Gene

Gene Ontology (GO) - Molecular Function for MED8 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0001104 RNA polymerase II transcription cofactor activity IEA --
GO:0005515 protein binding IPI 12149480
genes like me logo Genes that share ontologies with MED8: view
genes like me logo Genes that share phenotypes with MED8: view

Animal Model Products

CRISPR Products

Inhibitory RNA Products

Clone Products

  • Addgene plasmids for MED8
  • Applied Biological Materials Clones for MED8
  • Vectors and viruses for ORF, Lenti, Retro, Adenovirus, AAV, and more

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for MED8 Gene

Localization for MED8 Gene

Subcellular locations from UniProtKB/Swiss-Prot for MED8 Gene

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for MED8 gene
Compartment Confidence
nucleus 5
cytosol 3
golgi apparatus 2

Subcellular locations from the

Human Protein Atlas (HPA)
  • Golgi apparatus (2)
  • Nucleoplasm (2)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for MED8 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IEA --
GO:0005654 nucleoplasm TAS --
GO:0016592 mediator complex IDA 14638676
genes like me logo Genes that share ontologies with MED8: view

Pathways & Interactions for MED8 Gene

genes like me logo Genes that share pathways with MED8: view

Pathways by source for MED8 Gene

UniProtKB/Swiss-Prot Q96G25-MED8_HUMAN

  • Pathway: Protein modification; protein ubiquitination.

Gene Ontology (GO) - Biological Process for MED8 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006351 transcription, DNA-templated IEA --
GO:0006355 regulation of transcription, DNA-templated IEA --
GO:0006357 regulation of transcription from RNA polymerase II promoter IEA --
GO:0006367 transcription initiation from RNA polymerase II promoter TAS --
GO:0016567 protein ubiquitination IEA --
genes like me logo Genes that share ontologies with MED8: view

No data available for SIGNOR curated interactions for MED8 Gene

Drugs & Compounds for MED8 Gene

No Compound Related Data Available

Transcripts for MED8 Gene

Unigene Clusters for MED8 Gene

Mediator complex subunit 8:
Representative Sequences:

CRISPR Products

Inhibitory RNA Products

Clone Products

  • Addgene plasmids for MED8
  • Applied Biological Materials Clones for MED8
  • Vectors and viruses for ORF, Lenti, Retro, Adenovirus, AAV, and more

Alternative Splicing Database (ASD) splice patterns (SP) for MED8 Gene

ExUns: 1 ^ 2a · 2b ^ 3a · 3b · 3c ^ 4 ^ 5 ^ 6a · 6b · 6c ^ 7a · 7b · 7c ^ 8
SP1: - -
SP2: -
SP3: -
SP4:
SP5: - -
SP6:

Relevant External Links for MED8 Gene

GeneLoc Exon Structure for
MED8
ECgene alternative splicing isoforms for
MED8

Expression for MED8 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and CGAP SAGE for MED8 Gene

Protein differential expression in normal tissues from HIPED for MED8 Gene

This gene is overexpressed in Breast (40.1) and Peripheral blood mononuclear cells (15.1).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MaxQB, and MOPED for MED8 Gene



Protein tissue co-expression partners for MED8 Gene

NURSA nuclear receptor signaling pathways regulating expression of MED8 Gene:

MED8

SOURCE GeneReport for Unigene cluster for MED8 Gene:

Hs.301756

Evidence on tissue expression from TISSUES for MED8 Gene

  • Liver(4.2)
  • Nervous system(3)
genes like me logo Genes that share expression patterns with MED8: view

Primer Products

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , mRNA differential expression in normal tissues , mRNA Expression by UniProt/SwissProt and Phenotype-based relationships between genes and organs from Gene ORGANizer for MED8 Gene

Orthologs for MED8 Gene

This gene was present in the common ancestor of animals.

Orthologs for MED8 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia MED8 33 34
  • 99.88 (n)
cow
(Bos Taurus)
Mammalia MED8 34
  • 96 (a)
OneToOne
dog
(Canis familiaris)
Mammalia MED8 33 34
  • 91.54 (n)
oppossum
(Monodelphis domestica)
Mammalia MED8 34
  • 91 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Med8 33 16 34
  • 90.42 (n)
platypus
(Ornithorhynchus anatinus)
Mammalia MED8 34
  • 89 (a)
OneToOne
chicken
(Gallus gallus)
Aves MED8 33 34
  • 77.99 (n)
lizard
(Anolis carolinensis)
Reptilia MED8 34
  • 76 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia med8 33
  • 70.29 (n)
Str.685 33
African clawed frog
(Xenopus laevis)
Amphibia Xl.8572 33
zebrafish
(Danio rerio)
Actinopterygii med8 33 34
  • 66.41 (n)
MED8 (2 of 2) 34
  • 62 (a)
OneToMany
Dr.15496 33
rainbow trout
(Oncorhynchus mykiss)
Actinopterygii Omy.6726 33
fruit fly
(Drosophila melanogaster)
Insecta MED8 33 34
  • 54.12 (n)
African malaria mosquito
(Anopheles gambiae)
Insecta MED8_ANOGA 33
  • 52.79 (n)
worm
(Caenorhabditis elegans)
Secernentea mdt-8 34
  • 21 (a)
OneToOne
sea squirt
(Ciona savignyi)
Ascidiacea CSA.2247 34
  • 41 (a)
OneToOne
Species where no ortholog for MED8 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rat (Rattus norvegicus)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for MED8 Gene

ENSEMBL:
Gene Tree for MED8 (if available)
TreeFam:
Gene Tree for MED8 (if available)

Paralogs for MED8 Gene

No data available for Paralogs for MED8 Gene

Variants for MED8 Gene

Sequence variations from dbSNP and Humsavar for MED8 Gene

SNP ID Clin Chr 01 pos Sequence Context AA Info Type
rs1000070970 -- 43,389,376(+) TCCCC(C/T)GCTAT intron-variant, upstream-variant-2KB
rs1000110023 -- 43,384,333(+) CCACT(C/T)CCTGA upstream-variant-2KB, utr-variant-3-prime
rs1000128151 -- 43,384,805(+) TTAAT(C/T)CTCAC intron-variant, upstream-variant-2KB, utr-variant-3-prime
rs1000520421 -- 43,383,443(+) GACAT(G/T)TCCAG downstream-variant-500B, upstream-variant-2KB
rs1000673940 -- 43,391,596(+) ATCAG(A/G)AATAT intron-variant, upstream-variant-2KB

Structural Variations from Database of Genomic Variants (DGV) for MED8 Gene

Variant ID Type Subtype PubMed ID
nsv546128 CNV loss 21841781

Variation tolerance for MED8 Gene

Residual Variation Intolerance Score: 52.6% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 0.72; 15.29% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for MED8 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
MED8

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for MED8 Gene

Disorders for MED8 Gene

Relevant External Links for MED8

Atlas of Genetics and Cytogenetics in Oncology and Haematology:
MED8

No disorders were found for MED8 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for MED8 Gene

Publications for MED8 Gene

  1. Mammalian mediator subunit mMED8 is an Elongin BC-interacting protein that can assemble with Cul2 and Rbx1 to reconstitute a ubiquitin ligase. (PMID: 12149480) Brower CS … Conaway RC (Proceedings of the National Academy of Sciences of the United States of America 2002) 2 3 4 60
  2. MED1/TRAP220 exists predominantly in a TRAP/ Mediator subpopulation enriched in RNA polymerase II and is required for ER-mediated transcription. (PMID: 15989967) Zhang X … Roeder RG (Molecular cell 2005) 3 4 60
  3. A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology. (PMID: 15175163) Sato S … Conaway RC (Molecular cell 2004) 3 4 60
  4. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PMID: 15489334) Gerhard DS … MGC Project Team (Genome research 2004) 3 4 60
  5. Identification of mammalian Mediator subunits with similarities to yeast Mediator subunits Srb5, Srb6, Med11, and Rox3. (PMID: 12584197) Sato S … Conaway JW (The Journal of biological chemistry 2003) 3 4 60

Products for MED8 Gene

  • Addgene plasmids for MED8

Sources for MED8 Gene

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