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MED4 Gene

protein-coding   GIFtS: 62
GCID: GC13M048627

Mediator Complex Subunit 4

(Previous names: vitamin D receptor interacting protein, mediator of RNA...)
(Previous symbol: VDRIP)
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(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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Aliases
Mediator Complex Subunit 41 2 3     Vitamin D Receptor Interacting Protein1
VDRIP1 2 3     TRAP362
DRIP362 3 5     Mediator Of RNA Polymerase II Transcription Subunit 42
Activator-Recruited Cofactor 36 KDa Component2 3     Mediator, 34-KD Subunit, Homolog2
Vitamin D3 Receptor-Interacting Protein Complex 36 KDa Component2 3     TRAP/SMCC/PC2 Subunit P362
ARC362 3     Vitamin D Receptor-Interacting Protein, 36-KD2
HSPC1262 5     TRAP/SMCC/PC2 Subunit P36 Subunit3
Mediator Of RNA Polymerase II Transcription, Subunit 4 Homolog (S.
Cerevisiae)1
     

External Ids:    HGNC: 179031   Entrez Gene: 290792   Ensembl: ENSG000001361467   OMIM: 6057185   UniProtKB: Q9NPJ63   

Export aliases for MED4 gene to outside databases

Previous GC identifers: GC13M047550 GC13M029442


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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Entrez Gene summary for MED4 Gene:
This gene encodes a component of the Mediator complex. The Mediator complex interacts with DNA-binding
gene-specific transcription factors to modulate transcription by RNA polymerase II. Alternatively spliced
transcript variants encoding multiple isoforms have been observed for this gene. (provided by RefSeq, Jul 2012)

GeneCards Summary for MED4 Gene:
MED4 (mediator complex subunit 4) is a protein-coding gene. GO annotations related to this gene include receptor activity and ligand-dependent nuclear receptor transcription coactivator activity.

UniProtKB/Swiss-Prot: MED4_HUMAN, Q9NPJ6
Function: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all
RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific
regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by
direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional
preinitiation complex with RNA polymerase II and the general transcription factors

Gene Wiki entry for MED4 Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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RefSeq DNA sequence at NCBI GenBank:
NC_000013.10  NC_018924.2  NT_024524.15  
Regulatory elements:
   Regulatory transcription factor binding sites in the MED4 gene promoter:
         c-Fos   STAT5B   AP-1   p300   POU3F1   Nkx2-5   SRY   PPAR-gamma1   PPAR-gamma2   c-Jun   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidMED4 promoter sequence
   Search Chromatin IP Primers for MED4

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat MED4


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 13q14.2   Ensembl cytogenetic band:  13q14.2   HGNC cytogenetic band: 13q14.12

MED4 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
MED4 gene location

GeneLoc information about chromosome 13         GeneLoc Exon Structure

GeneLoc location for GC13M048627:  view genomic region     (about GC identifiers)

Start:
48,627,459 bp from pter      End:
48,669,267 bp from pter
Size:
41,809 bases      Orientation:
minus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, Cloud-Clone Corp., eBioscience, and/or antibodies-online,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, Cloud-Clone Corp., eBioscience, and/or antibodies-online, Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, Cloud-Clone Corp, antibodies-online, and/or others.)
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UniProtKB/Swiss-Prot: MED4_HUMAN, Q9NPJ6 (See protein sequence)
Recommended Name: Mediator of RNA polymerase II transcription subunit 4  
Size: 270 amino acids; 29745 Da
Subunit: Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11,
MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26,
MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct
module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this
module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or
more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP
Secondary accessions: B4DX67 Q53GB4 Q53H68 Q5T912 Q6FHC4 Q6IA79 Q9BS95 Q9NYR5
Alternative splicing: 2 isoforms:  Q9NPJ6-1   Q9NPJ6-2   

Explore the universe of human proteins at neXtProt for MED4: NX_Q9NPJ6

Explore proteomics data for MED4 at MOPED

Post-translational modifications: 

  • Ubiquitination2 at Lys55, Lys81, Lys98, Lys112, Lys150
  • Modification sites at PhosphoSitePlus

  • See MED4 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins (2 alternative transcripts): 
    NP_001257558.1  NP_054885.1  

    ENSEMBL proteins: 
     ENSP00000258648   ENSP00000367849   ENSP00000413595  
    Reactome Protein details: Q9NPJ6

    MED4 Human Recombinant Protein Products:

    Browse Purified and Recombinant Proteins at EMD Millipore
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    Browse recombinant and purified proteins available from Enzo Life Sciences
    OriGene Purified Protein for MED4
    OriGene Protein Over-expression Lysate for MED4
    OriGene MassSpec for MED4
    OriGene Custom Protein Services for MED4
    GenScript Custom Purified and Recombinant Proteins Services for MED4
    Novus Biologicals MED4 Proteins
    Novus Biologicals MED4 Lysate
    Browse Sino Biological Recombinant Proteins
    Browse Sino Biological Cell Lysates
    ProSpec Recombinant Protein for MED4
    Cloud-Clone Corp. Proteins for MED4

     
    Search eBioscience for Proteins for MED4 

     
    antibodies-online proteins for MED4 (12 products) 

     
    antibodies-online peptides for MED4

    MED4 Antibody Products:

    Browse EMD Millipore's Extensive Line of Mono- and Polyclonal Antibodies
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    Novus Biologicals MED4 Antibodies
    Abcam antibodies for MED4
    Cloud-Clone Corp. Antibodies for MED4
    ThermoFisher Antibody for MED4
    LSBio Antibodies in human, mouse, rat for MED4
    antibodies-online antibodies for MED4 (21 products) 

    MED4 Assay Products:

    Browse Kits and Assays available from EMD Millipore
    OriGene Custom Assay Services for MED4
    Browse R&D Systems for biochemical assays
    GenScript Custom Assay Services for MED4
    Browse Enzo Life Sciences for kits & assays
    Cloud-Clone Corp. ELISAs for MED4
    Cloud-Clone Corp. CLIAs for MED4
    Search eBioscience for ELISAs for MED4 
    antibodies-online kits for MED4 (20 products) 


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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    1 InterPro protein domain:
     IPR019258 Mediator_Med4

    Graphical View of Domain Structure for InterPro Entry Q9NPJ6

    ProtoNet protein and cluster: Q9NPJ6

    UniProtKB/Swiss-Prot: MED4_HUMAN, Q9NPJ6
    Similarity: Belongs to the Mediator complex subunit 4 family


    Find genes that share domains with MED4           About GenesLikeMe


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, Vector BioLabs and/or Addgene, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Flow cytometry from eBioscience, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Molecular Function:

         UniProtKB/Swiss-Prot Summary: MED4_HUMAN, Q9NPJ6
    Function: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all
    RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific
    regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by
    direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional
    preinitiation complex with RNA polymerase II and the general transcription factors

         Gene Ontology (GO): Selected molecular function terms (see all 7):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0001104RNA polymerase II transcription cofactor activity IDA10882111
    GO:0003712transcription cofactor activity IDA12218053
    GO:0004872receptor activity IDA12218053
    GO:0005515protein binding IPI14638676
    GO:0030374ligand-dependent nuclear receptor transcription coactivator activity NAS10235266
         
    Find genes that share ontologies with MED4           About GenesLikeMe


    Phenotypes:
         7 GenomeRNAi human phenotypes for MED4:
     Decreased Salmonella enterica   Decreased Salmonella enterica   Decreased Salmonella enterica   Decreased Salmonella enterica  
     Decreased Salmonella enterica   Decreased Salmonella-containin  Decreased p24 protein expressi 

    Animal Models:
       inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for MED4
       inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for MED4

       genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for MED4
       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for MED4

    miRNA
    Products:
        
    miRTarBase miRNAs that target MED4:
    hsa-mir-23b-3p (MIRT023475), hsa-mir-186-5p (MIRT021128), hsa-mir-425-5p (MIRT016676)

    Block miRNA regulation of human, mouse, rat MED4 using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate MED4 (see all 12):
    hsa-miR-3163 hsa-miR-576-5p hsa-miR-520d-5p hsa-miR-548e hsa-miR-4263 hsa-miR-190b hsa-miR-1305 hsa-miR-421
    SwitchGear 3'UTR luciferase reporter plasmidMED4 3' UTR sequence
    Inhib. RNA
    Products:
        
    OriGene RNAi products in human, mouse, rat for MED4
    Predesigned siRNA for gene silencing in human, mouse, rat MED4

    Gene Editing
    Products:
    DNA2.0 Custom Protein Engineering Service for MED4

    Clone
    Products:
         
    OriGene clones in human, mouse for MED4 (see all 7)
    OriGene ORF clones in mouse, rat for MED4
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector: MED4 (NM_014166)
    Sino Biological Human cDNA Clone for MED4
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for MED4
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat MED4
    Addgene plasmids for MED4 

    Cell Line
    Products:
         
    GenScript Custom overexpressing Cell Line Services for MED4
    Browse ESI BIO Cell Lines and PureStem Progenitors for MED4 
    In Situ Assay
    Products:
       

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for MED4


    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Subcellular locations from UniProtKB/Swiss-Prot
    MED4_HUMAN, Q9NPJ6: Nucleus
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    nucleus5
    cytosol2
    extracellular1

    Gene Ontology (GO): 4 cellular component terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634nucleus IDA--
    GO:0005654nucleoplasm TAS--
    GO:0005730NOT nucleolus IDA--
    GO:0016592mediator complex IDA10882111

    Find genes that share ontologies with MED4           About GenesLikeMe


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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    SuperPaths for MED4 About   (see all 6)  
    See pathways by source

    SuperPathContained pathways About
    1Fatty acid, triacylglycerol, and ketone body metabolism
    Fatty acid, triacylglycerol, and ketone body metabolism0.65
    PPARA Activates Gene Expression0.63
    Regulation of Lipid Metabolism by Peroxisome proliferator-activated receptor alpha (PPARalpha)0.65
    Transcriptional Regulation of White Adipocyte Differentiation0.38
    2Gene Expression
    Generic Transcription Pathway0.40
    Gene Expression0.40
    3Metabolism
    Metabolism0.38
    Metabolism of lipids and lipoproteins0.37
    4L1CAM interactions
    Developmental Biology0.63
    5Transcription Ligand Dependent Transcription of Retinoid Target genes
    Transcription Ligand Dependent Transcription of Retinoid Target genes


    Find genes that share SuperPaths with MED4           About GenesLikeMe

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways


    1 GeneGo (Thomson Reuters) Pathway for MED4
        Transcription Ligand-Dependent Transcription of Retinoid-Target genes


    3 Reactome Pathways for MED4
        Transcriptional regulation of white adipocyte differentiation
    Generic Transcription Pathway
    PPARA activates gene expression


    1 Kegg Pathway  (Kegg details for MED4):
        Thyroid hormone signaling pathway

        Pathway & Disease-focused RT2 Profiler PCR Arrays including MED4: 
              Nitric Oxide Signaling Pathway in human mouse rat
              Nuclear Receptors & Coregulators in human mouse rat

    Interactions:

        GeneGlobe Interaction Network for MED4

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    Selected Interacting proteins for MED4 (Q9NPJ61, 2, 3 ENSP000002586484) via UniProtKB, MINT, STRING, and/or I2D (see all 907)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    TNFAIP8O953792, 3, ENSP000004085344MINT-66580 I2D: score=5 STRING: ENSP00000408534
    EXOC2Q96KP12, 3MINT-7945693 MINT-7947479 I2D: score=4 
    MED9Q9NWA01, 3, ENSP000002687114EBI-394607,EBI-394653 I2D: score=3 STRING: ENSP00000268711
    PRPF3O433952, 3MINT-7947479 I2D: score=4 
    ATF7IPQ6VMQ62, 3MINT-61901 I2D: score=3 
    About this table

    Gene Ontology (GO): Selected biological process terms (see all 7):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006357regulation of transcription from RNA polymerase II promoter ----
    GO:0006366transcription from RNA polymerase II promoter IDA10882111
    GO:0006367transcription initiation from RNA polymerase II promoter TAS--
    GO:0010467gene expression TAS--
    GO:0030518intracellular steroid hormone receptor signaling pathway IDA11867769

    Find genes that share ontologies with MED4           About GenesLikeMe



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Browse Tocris compounds for MED4

    2 Novoseek inferred chemical compound relationships for MED4 gene    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    vitamin d 68.1 7 17229570 (1), 10753860 (1), 15886699 (1), 12403843 (1) (see all 6)
    steroid 37.8 3 17229570 (1), 12403843 (1), 15577143 (1)



    Find genes that share compounds with MED4           About GenesLikeMe



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, and/or Addgene, Primers from OriGene, and/or QIAGEN, Flow cytometry from eBioscience )
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    REFSEQ mRNAs for MED4 gene (2 alternative transcripts): 
    NM_001270629.1  NM_014166.3  

    Unigene Cluster for MED4:

    Mediator complex subunit 4
    Hs.181112  [show with all ESTs]
    Unigene Representative Sequence: NM_014166
    4 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000495013 ENST00000258648(uc001vby.1 uc010tgf.1) ENST00000378586
    ENST00000417167
    miRNA
    Products:
         
    Block miRNA regulation of human, mouse, rat MED4 using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate MED4 (see all 12):
    hsa-miR-3163 hsa-miR-576-5p hsa-miR-520d-5p hsa-miR-548e hsa-miR-4263 hsa-miR-190b hsa-miR-1305 hsa-miR-421
    SwitchGear 3'UTR luciferase reporter plasmidMED4 3' UTR sequence
    Inhib. RNA
    Products:
         
    OriGene RNAi products in human, mouse, rat for MED4
    Predesigned siRNA for gene silencing in human, mouse, rat MED4
    Clone
    Products:
         
    OriGene clones in human, mouse for MED4 (see all 7)
    OriGene ORF clones in mouse, rat for MED4
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector: MED4 (NM_014166)
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for MED4
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat MED4
    Addgene plasmids for MED4 
    Primer
    Products:
        
    OriGene qPCR primer pairs and template standards for MED4
    OriGene qSTAR qPCR primer pairs in human, mouse for MED4
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat MED4
      QuantiTect SYBR Green Assays in human, mouse, rat MED4
      QuantiFast Probe-based Assays in human, mouse, rat MED4

    Additional mRNA sequence: 

    AF161475.1 AF230381.1 AF300618.1 AK001934.1 AK222713.1 AK223017.1 AK301835.1 BC005189.1 
    CR457276.1 CR541830.1 

    11 DOTS entries:

    DT.454773  DT.100811867  DT.100822742  DT.100822741  DT.91680123  DT.92025551  DT.95362377  DT.99932104 
    DT.92450372  DT.97781406  DT.86839601 

    Selected AceView cDNA sequences (see all 362):

    BQ921201 CB243881 CA412587 CD514515 BM856625 BG499588 BM975306 CD703849 
    BI064312 BG035974 CA422183 AI049615 AW235969 NM_014166 CR598896 BF445860 
    BM839881 AA454015 BQ574601 AA918389 Z38805 AI261543 BU733462 AW629268 

    GeneLoc Exon Structure


    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
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    MED4 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: GGAAATTGTC
    MED4 Expression
    About this image


    MED4 expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database
     selected tissues (see all 7) fully expand
     
     Brain (Nervous System)    fully expand to see all 4 entries
             Cerebral Cortex
     
     Ovary (Reproductive System)    fully expand to see all 2 entries
             Ovarian Mesenchymal Stroma Cells Ovary Interstitium
             Oviduct
     
     Testis (Reproductive System)
             Leydig Cells Testis Interstitium
     
     Trophoblast (Extraembryonic Tissues)
             Trophoblast Cells Trophoblast
     
     Pancreas (Endocrine System)
             Islets of Langerhans
    MED4 Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    MED4 Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.181112
        Pathway & Disease-focused RT2 Profiler PCR Arrays including MED4: 
              Nitric Oxide Signaling Pathway in human mouse rat
              Nuclear Receptors & Coregulators in human mouse rat

    Primer
    Products:
    OriGene qPCR primer pairs and template standards for MED4
    OriGene qSTAR qPCR primer pairs in human, mouse for MED4
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat MED4
    QuantiTect SYBR Green Assays in human, mouse, rat MED4
    QuantiFast Probe-based Assays in human, mouse, rat MED4
    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for MED4

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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    This gene was present in the common ancestor of animals.

    Orthologs for MED4 gene from Selected species (see all 16)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Med41 , 5 mediator of RNA polymerase II transcription, subunit more5
    mediator complex subunit 41
    87.21(n)1
    94.57(a)1
      14 (39.05 cM)5
    673811  NM_026119.31  NP_080395.11 
     735100495 
    chicken
    (Gallus gallus)
    Aves MED41 mediator complex subunit 4 81.99(n)
    91.49(a)
      418859  XM_417054.4  XP_417054.1 
    lizard
    (Anolis carolinensis)
    Reptilia MED46
    mediator complex subunit 4
    89(a)
    1 ↔ 1
    1(136981731-136997875)
    African clawed frog
    (Xenopus laevis)
    Amphibia Xl.87382 Xenopus laevis transcribed sequence with moderate similarity more 76.22(n)    CF520285.1 
    zebrafish
    (Danio rerio)
    Actinopterygii Dr.180602 Transcribed sequence with moderate similarity to protein more 70.84(n)    CK353711.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta MED41 Mediator complex subunit 4 53.15(n)
    45.95(a)
      38799  NM_139837.3  NP_648094.1 
    worm
    (Caenorhabditis elegans)
    Secernentea mdt-46
    Protein MDT-4 (mdt-4) mRNA, complete cds
    27(a)
    1 ↔ 1
    II(4942107-4943942) WBGene00007012


    ENSEMBL Gene Tree for MED4 (if available)
    TreeFam Gene Tree for MED4 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
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    Paralogs for MED4 gene
    6 SIMAP similar genes for MED4 using alignment to 3 protein entries:     MED4_HUMAN (see all proteins):
    OK/SW-cl.41    DNAPTP3    FAM138C    FLJ20555    KIAA1651    FP6651

    Find genes that share paralogs with MED4           About GenesLikeMe



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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    Selected SNPs for MED4 (see all 380)    About this table                                 

    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 13 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1438357861,2
    --48654670(+) TTCTAC/TACTTT 2 -- int10--------
    rs1384755921,2
    --48654749(+) AATTAC/TTCAAA 2 -- int10--------
    rs1881049261,2
    --48654796(+) TATGCC/TGCAAG 2 -- int10--------
    rs95679921,2
    C,F,H--48654837(+) GAAAGG/AACTGG 2 -- int18Minor allele frequency- A:0.09NS EA CSA WA NA 660
    rs112993251,2
    C--48654878(+) AAATC-/TTTTTT 2 -- int1 trp31Minor allele frequency- T:0.00NA 2
    rs1415832861,2
    --48654951(+) TAAAAC/TCTTTT 2 -- int10--------
    rs1461699481,2
    --48654997(+) TAATTC/TGTGTA 2 -- int10--------
    rs1928692271,2
    --48655022(+) AGGAGA/CAGGTA 2 -- int10--------
    rs1846331041,2
    --48655063(+) ATAGCA/GGTATG 2 -- int10--------
    rs73238211,2
    C,F,H--48655130(+) TGCAGA/CTTATC 2 -- int117Minor allele frequency- C:0.04NS EA NA WA CSA 789

    HapMap Linkage Disequilibrium report for MED4 (48627459 - 48669267 bp)

    Structural Variations
         Database of Genomic Variants (DGV) 2 variations for MED4:    About this table    
    Variant IDTypeSubtypePubMed ID
    esv2676750CNV Deletion23128226
    nsv832601CNV Gain17160897

    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing MED4
    DNA2.0 Custom Variant and Variant Library Synthesis for MED4

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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    OMIM gene information: 605718    OMIM disorders: --


    Find genes that share disorders with MED4           About GenesLikeMe

    Genetic Association Database (GAD): MED4
    Human Genome Epidemiology (HuGE) Navigator: MED4 (1 document)

    Export disorders for MED4 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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    PubMed articles for MED4 gene, integrated from 10 sources (see all 61):
    (articles sorted by number of sources associating them with MED4)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells. (PubMed id 11042152)1, 2, 3 Zhang Q.-H.... Chen Z. (Genome Res. 2000)
    2. Ligand-dependent transcription activation by nuclear receptors requires the DRIP complex. (PubMed id 10235266)1, 2, 3 Rachez C.... Freedman L.P. (Nature 1999)
    3. MED1/TRAP220 exists predominantly in a TRAP/Mediator subpopulation enriched in RNA polymerase II and is required for ER-mediated transcription. (PubMed id 15989967)1, 2 Zhang X....Roeder R.G. (Mol. Cell 2005)
    4. The DNA sequence and analysis of human chromosome 13. (PubMed id 15057823)1, 2 Dunham A.... Ross M.T. (Nature 2004)
    5. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (Nat. Genet. 2004)
    6. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S.... Malek J. (Genome Res. 2004)
    7. A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology. (PubMed id 15175163)1, 2 Sato S.... Conaway R.C. (Mol. Cell 2004)
    8. The USA-derived transcriptional coactivator PC2 is a submodule of TRAP/SMCC and acts synergistically with other PCs. (PubMed id 10882111)1, 2 Malik S.... Roeder R.G. (Mol. Cell 2000)
    9. Composite co-activator ARC mediates chromatin-directed transcriptional activation. (PubMed id 10235267)1, 2 Naeaer A.M....Tjian R. (Nature 1999)
    10. Identification of the hypoxia-inducible factor 2I+ nuclear interactome in melanoma cells reveals master proteins involved in melanoma development. (PubMed id 23275444)1 Steunou A.L....Nieto L. (amp 2013)

    (in PubMed, OMIM, and NCBI Bookshelf)
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     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Entrez Gene: 29079 HGNC: 17903 AceView: VDRIP Ensembl:ENSG00000136146 euGenes: HUgn29079
    ECgene: MED4 Kegg: 29079 H-InvDB: MED4

    (According to HUGE)
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      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
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    NameDescription
    PharmGKB entry for MED4 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
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    Patent Information for MED4 gene:
    Search GeneIP for patents involving MED4

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, eBioscience, antibodies-online, and/or others, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Addgene, Cell lines from GenScript, and ESI BIO, Flow cytometery from eBioscience, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
    Advanced Cell Diagnostics, Animal models from inGenious Targeting Laboratory, genOway)
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