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Aliases for MED28 Gene

Aliases for MED28 Gene

  • Mediator Complex Subunit 28 2 3 4 5
  • Merlin And Grb2-Interacting Cytoskeletal Protein 3 4
  • Tumor Angiogenesis Marker EG-1 3 4
  • Endothelial-Derived Protein 1 3 4
  • Magicin 3 4
  • EG1 3 4
  • Mediator Of RNA Polymerase II Transcription, Subunit 28 Homolog (S. Cerevisiae) 2
  • Mediator Of RNA Polymerase II Transcription, Subunit 28 Homolog 3
  • Mediator Of RNA Polymerase II Transcription Subunit 28 3
  • Endothelial-Derived Gene 1 3
  • 1500003D12Rik 3

External Ids for MED28 Gene

Previous GeneCards Identifiers for MED28 Gene

  • GC04P017293
  • GC04P016970

Summaries for MED28 Gene

GeneCards Summary for MED28 Gene

MED28 (Mediator Complex Subunit 28) is a Protein Coding gene. Diseases associated with MED28 include Chronic Intestinal Vascular Insufficiency. Among its related pathways are Regulation of lipid metabolism by Peroxisome proliferator-activated receptor alpha (PPARalpha) and Metabolism. GO annotations related to this gene include actin binding.

UniProtKB/Swiss-Prot for MED28 Gene

  • Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May be part of a complex containing NF2/merlin that participates in cellular signaling to the actin cytoskeleton downstream of tyrosine kinase signaling pathways.

Gene Wiki entry for MED28 Gene

No data available for Entrez Gene Summary , CIViC summary , Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for MED28 Gene

Genomics for MED28 Gene

Regulatory Elements for MED28 Gene

Enhancers for MED28 Gene
GeneHancer Identifier Enhancer Score Enhancer Sources Gene-Enhancer Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Gene Targets for Enhancer
GH04G017575 1.5 FANTOM5 ENCODE 30.8 -36.0 -35998 5.3 HDGF PKNOX1 FOXA2 MLX ARNT AGO1 WRNIP1 ARID4B SIN3A DMAP1 LAP3 MED28 DCAF16 ENSG00000249502 NCAPG QDPR LCORL GC04M017599
GH04G018019 1.1 ENCODE 22 +406.9 406869 3.4 HDGF FOXA2 PKNOX1 CREB3L1 ARNT WRNIP1 ZFP64 ARID4B SIN3A DMAP1 DCAF16 MED28 LCORL KRT18P63
GH04G017807 1.4 Ensembl ENCODE 11.7 +196.5 196521 7.6 HNRNPUL1 HDGF PKNOX1 MLX CREB3L1 WRNIP1 ARID4B SIN3A DMAP1 ZNF2 DCAF16 MED28 LCORL NCAPG GC04M017884
GH04G017613 1.1 ENCODE 11 +0.0 25 2.0 FOXA2 MLX ZFP64 ARID4B SIN3A DMAP1 YY1 ZNF207 FOS KLF13 LAP3 MED28 QDPR PIR38754
GH04G017548 1 Ensembl ENCODE 10.9 -65.3 -65269 1.3 HDAC1 CTCF ATF1 IRF2 SAP130 HINFP ARNT ARID4B SIN3A RAD21 QDPR DCAF16 NCAPG MED28 ENSG00000249502 GC04M017552 CLRN2
- Elite enhancer and/or Elite enhancer-gene association Download GeneHancer data dump

Enhancers around MED28 on UCSC Golden Path with GeneCards custom track

Promoters for MED28 Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters
ENSR00000166438 -28 2401 FOXA2 MLX ZFP64 ARID4B SIN3A DMAP1 YY1 FOS SP3 ZHX2

Genomic Location for MED28 Gene

Chromosome:
4
Start:
17,614,628 bp from pter
End:
17,634,105 bp from pter
Size:
19,478 bases
Orientation:
Plus strand

Genomic View for MED28 Gene

Genes around MED28 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
MED28 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for MED28 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for MED28 Gene

Proteins for MED28 Gene

  • Protein details for MED28 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q9H204-MED28_HUMAN
    Recommended name:
    Mediator of RNA polymerase II transcription subunit 28
    Protein Accession:
    Q9H204
    Secondary Accessions:
    • Q9BZJ5

    Protein attributes for MED28 Gene

    Size:
    178 amino acids
    Molecular mass:
    19520 Da
    Quaternary structure:
    • Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Forms a ternary complex with NF2/merlin and GRB2. Binds to actin.
    SequenceCaution:
    • Sequence=AAK11563.1; Type=Frameshift; Positions=165; Evidence={ECO:0000305};

neXtProt entry for MED28 Gene

Post-translational modifications for MED28 Gene

  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Other Protein References for MED28 Gene

ENSEMBL proteins:
REFSEQ proteins:

No data available for DME Specific Peptides for MED28 Gene

Domains & Families for MED28 Gene

Gene Families for MED28 Gene

Protein Domains for MED28 Gene

InterPro:
ProtoNet:

Suggested Antigen Peptide Sequences for MED28 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

Q9H204

UniProtKB/Swiss-Prot:

MED28_HUMAN :
  • Belongs to the Mediator complex subunit 28 family.
Family:
  • Belongs to the Mediator complex subunit 28 family.
genes like me logo Genes that share domains with MED28: view

Function for MED28 Gene

Molecular function for MED28 Gene

UniProtKB/Swiss-Prot Function:
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May be part of a complex containing NF2/merlin that participates in cellular signaling to the actin cytoskeleton downstream of tyrosine kinase signaling pathways.
UniProtKB/Swiss-Prot Induction:
Up-regulated by endothelial cells when exposed to tumor conditional media.

Gene Ontology (GO) - Molecular Function for MED28 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003779 actin binding IEA --
GO:0005515 protein binding IPI 10656681
genes like me logo Genes that share ontologies with MED28: view
genes like me logo Genes that share phenotypes with MED28: view

Animal Models for MED28 Gene

MGI Knock Outs for MED28:

Animal Model Products

CRISPR Products

miRNA for MED28 Gene

miRTarBase miRNAs that target MED28

Inhibitory RNA Products

Clone Products

  • Addgene plasmids for MED28

Flow Cytometry Products

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for MED28 Gene

Localization for MED28 Gene

Subcellular locations from UniProtKB/Swiss-Prot for MED28 Gene

Nucleus. Cytoplasm. Membrane; Peripheral membrane protein. Note=According to PubMed:15467741, it is cytoplasmic and mainly membrane-associated.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for MED28 gene
Compartment Confidence
nucleus 5
cytosol 3
plasma membrane 2
extracellular 2
cytoskeleton 2

Gene Ontology (GO) - Cellular Components for MED28 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IEA --
GO:0005654 nucleoplasm IDA --
GO:0005737 cytoplasm IEA --
GO:0005856 cytoskeleton IEA --
GO:0016020 membrane IEA --
genes like me logo Genes that share ontologies with MED28: view

Pathways & Interactions for MED28 Gene

genes like me logo Genes that share pathways with MED28: view

SIGNOR curated interactions for MED28 Gene

Is activated by:

Gene Ontology (GO) - Biological Process for MED28 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006351 transcription, DNA-templated IEA --
GO:0006355 regulation of transcription, DNA-templated IEA --
GO:0019827 stem cell population maintenance IEA --
GO:0051151 negative regulation of smooth muscle cell differentiation IEA --
genes like me logo Genes that share ontologies with MED28: view

Transcripts for MED28 Gene

Unigene Clusters for MED28 Gene

Mediator complex subunit 28:
Representative Sequences:

CRISPR Products

Inhibitory RNA Products

Clone Products

  • Addgene plasmids for MED28

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for MED28 Gene

No ASD Table

Relevant External Links for MED28 Gene

GeneLoc Exon Structure for
MED28
ECgene alternative splicing isoforms for
MED28

Expression for MED28 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and CGAP SAGE for MED28 Gene

Protein differential expression in normal tissues from HIPED for MED28 Gene

This gene is overexpressed in Placenta (15.6), Spleen (14.4), Ovary (13.5), and Fetal ovary (7.7).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MaxQB, and MOPED for MED28 Gene



Protein tissue co-expression partners for MED28 Gene

NURSA nuclear receptor signaling pathways regulating expression of MED28 Gene:

MED28

SOURCE GeneReport for Unigene cluster for MED28 Gene:

Hs.731966

mRNA Expression by UniProt/SwissProt for MED28 Gene:

Q9H204-MED28_HUMAN
Tissue specificity: Widely expressed. Highly expressed in vascular tissues such as placenta, testis and liver.

Evidence on tissue expression from TISSUES for MED28 Gene

  • Lung(4.4)
  • Nervous system(4.1)
  • Intestine(2.2)
  • Blood(2.1)
  • Muscle(2)
genes like me logo Genes that share expression patterns with MED28: view

Primer Products

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , mRNA differential expression in normal tissues and Phenotype-based relationships between genes and organs from Gene ORGANizer for MED28 Gene

Orthologs for MED28 Gene

This gene was present in the common ancestor of animals.

Orthologs for MED28 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia MED28 34 35
  • 99.44 (n)
cow
(Bos Taurus)
Mammalia MED28 34 35
  • 88.76 (n)
dog
(Canis familiaris)
Mammalia MED28 34 35
  • 88.57 (n)
mouse
(Mus musculus)
Mammalia Med28 34 16 35
  • 87.83 (n)
rat
(Rattus norvegicus)
Mammalia Med28 34
  • 87.64 (n)
oppossum
(Monodelphis domestica)
Mammalia MED28 35
  • 74 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia MED28 35
  • 71 (a)
OneToOne
chicken
(Gallus gallus)
Aves MED28 34 35
  • 70.83 (n)
lizard
(Anolis carolinensis)
Reptilia MED28 35
  • 89 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia med28 34
  • 73.51 (n)
Str.20230 34
zebrafish
(Danio rerio)
Actinopterygii med28 34 35
  • 74.56 (n)
wufc74b11 34
rainbow trout
(Oncorhynchus mykiss)
Actinopterygii Omy.13792 34
fruit fly
(Drosophila melanogaster)
Insecta MED28 34 35
  • 49.87 (n)
sea squirt
(Ciona savignyi)
Ascidiacea -- 35
  • 39 (a)
OneToOne
Species where no ortholog for MED28 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for MED28 Gene

ENSEMBL:
Gene Tree for MED28 (if available)
TreeFam:
Gene Tree for MED28 (if available)

Paralogs for MED28 Gene

No data available for Paralogs for MED28 Gene

Variants for MED28 Gene

Sequence variations from dbSNP and Humsavar for MED28 Gene

SNP ID Clin Chr 04 pos Sequence Context AA Info Type
rs1000071170 -- 17,616,660(+) TTCCT(C/T)AGTGG intron-variant
rs1000164504 -- 17,625,985(+) AATCA(A/G)TATTG downstream-variant-500B
rs1000553182 -- 17,616,105(+) ACAGG(C/T)ACCTG intron-variant
rs1000901348 -- 17,621,336(+) GATCT(C/G)ATGGC intron-variant
rs1001203515 -- 17,622,311(+) GAATT(A/G)AGATT intron-variant

Variation tolerance for MED28 Gene

Residual Variation Intolerance Score: 29.7% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 0.90; 18.65% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for MED28 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
MED28

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot and Structural Variations from Database of Genomic Variants (DGV) for MED28 Gene

Disorders for MED28 Gene

MalaCards: The human disease database

(1) MalaCards diseases for MED28 Gene - From: DISEASES and GeneCards

Disorder Aliases PubMed IDs
chronic intestinal vascular insufficiency
  • chronic mesenteric ischemia
- elite association - COSMIC cancer census association via MalaCards
Search MED28 in MalaCards View complete list of genes associated with diseases

Relevant External Links for MED28

Genetic Association Database (GAD)
MED28
Human Genome Epidemiology (HuGE) Navigator
MED28
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
MED28
genes like me logo Genes that share disorders with MED28: view

No data available for UniProtKB/Swiss-Prot and Genatlas for MED28 Gene

Publications for MED28 Gene

  1. Magicin, a novel cytoskeletal protein associates with the NF2 tumor suppressor merlin and Grb2. (PMID: 15467741) Wiederhold T. … Ramesh V. (Oncogene 2004) 2 3 4 22 64
  2. Identification of a novel endothelial-derived gene EG-1. (PMID: 11779215) Liu C. … Nguyen M.H. (Biochem. Biophys. Res. Commun. 2002) 2 3 4 22 64
  3. MED1/TRAP220 exists predominantly in a TRAP/Mediator subpopulation enriched in RNA polymerase II and is required for ER-mediated transcription. (PMID: 15989967) Zhang X. … Roeder R.G. (Mol. Cell 2005) 3 4 64
  4. A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology. (PMID: 15175163) Sato S. … Conaway R.C. (Mol. Cell 2004) 3 4 64
  5. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PMID: 15489334) Gerhard D.S. … Malek J. (Genome Res. 2004) 3 4 64

Products for MED28 Gene

Sources for MED28 Gene

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