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Aliases for MED28 Gene

Aliases for MED28 Gene

  • Mediator Complex Subunit 28 2 3 4 5
  • Merlin And Grb2-Interacting Cytoskeletal Protein 3 4
  • Tumor Angiogenesis Marker EG-1 3 4
  • Endothelial-Derived Protein 1 3 4
  • Magicin 3 4
  • EG1 3 4
  • Mediator Of RNA Polymerase II Transcription, Subunit 28 Homolog (S. Cerevisiae) 2
  • Mediator Of RNA Polymerase II Transcription, Subunit 28 Homolog 3
  • Endothelial-Derived Gene 1 3
  • 1500003D12Rik 3

External Ids for MED28 Gene

Previous GeneCards Identifiers for MED28 Gene

  • GC04P017293
  • GC04P016970

Summaries for MED28 Gene

GeneCards Summary for MED28 Gene

MED28 (Mediator Complex Subunit 28) is a Protein Coding gene. Diseases associated with MED28 include chronic intestinal vascular insufficiency and cholinergic urticaria. Among its related pathways are Metabolism and Regulation of lipid metabolism by Peroxisome proliferator-activated receptor alpha (PPARalpha). GO annotations related to this gene include actin binding.

UniProtKB/Swiss-Prot for MED28 Gene

  • Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May be part of a complex containing NF2/merlin that participates in cellular signaling to the actin cytoskeleton downstream of tyrosine kinase signaling pathways.

Gene Wiki entry for MED28 Gene

No data available for Entrez Gene Summary , Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for MED28 Gene

Genomics for MED28 Gene

Regulatory Elements for MED28 Gene

Promoters for MED28 Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters

ENSRs around MED28 on UCSC Golden Path with GeneCards custom track

Genomic Location for MED28 Gene

Chromosome:
4
Start:
17,614,628 bp from pter
End:
17,634,105 bp from pter
Size:
19,478 bases
Orientation:
Plus strand

Genomic View for MED28 Gene

Genes around MED28 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
MED28 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for MED28 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for MED28 Gene

Proteins for MED28 Gene

  • Protein details for MED28 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q9H204-MED28_HUMAN
    Recommended name:
    Mediator of RNA polymerase II transcription subunit 28
    Protein Accession:
    Q9H204
    Secondary Accessions:
    • Q9BZJ5

    Protein attributes for MED28 Gene

    Size:
    178 amino acids
    Molecular mass:
    19520 Da
    Quaternary structure:
    • Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Forms a ternary complex with NF2/merlin and GRB2. Binds to actin.
    SequenceCaution:
    • Sequence=AAK11563.1; Type=Frameshift; Positions=165; Evidence={ECO:0000305};

neXtProt entry for MED28 Gene

Proteomics data for MED28 Gene at MOPED

Post-translational modifications for MED28 Gene

  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Other Protein References for MED28 Gene

ENSEMBL proteins:
REFSEQ proteins:

Antibody Products

  • Santa Cruz Biotechnology (SCBT) Antibodies for MED28

No data available for DME Specific Peptides for MED28 Gene

Domains & Families for MED28 Gene

Gene Families for MED28 Gene

Protein Domains for MED28 Gene

InterPro:
ProtoNet:

Suggested Antigen Peptide Sequences for MED28 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

Q9H204

UniProtKB/Swiss-Prot:

MED28_HUMAN :
  • Belongs to the Mediator complex subunit 28 family.
Family:
  • Belongs to the Mediator complex subunit 28 family.
genes like me logo Genes that share domains with MED28: view

Function for MED28 Gene

Molecular function for MED28 Gene

UniProtKB/Swiss-Prot Function:
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May be part of a complex containing NF2/merlin that participates in cellular signaling to the actin cytoskeleton downstream of tyrosine kinase signaling pathways.
UniProtKB/Swiss-Prot Induction:
Up-regulated by endothelial cells when exposed to tumor conditional media.

Gene Ontology (GO) - Molecular Function for MED28 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003779 actin binding IEA --
genes like me logo Genes that share ontologies with MED28: view
genes like me logo Genes that share phenotypes with MED28: view

Animal Models for MED28 Gene

MGI Knock Outs for MED28:

Animal Model Products

miRNA for MED28 Gene

miRTarBase miRNAs that target MED28

Inhibitory RNA Products

In Situ Assay Products

Flow Cytometry Products

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for MED28 Gene

Localization for MED28 Gene

Subcellular locations from UniProtKB/Swiss-Prot for MED28 Gene

Nucleus. Cytoplasm. Membrane; Peripheral membrane protein. Note=According to PubMed:15467741, it is cytoplasmic and mainly membrane-associated.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for MED28 Gene COMPARTMENTS Subcellular localization image for MED28 gene
Compartment Confidence
nucleus 5
cytosol 3
cytoskeleton 2
extracellular 2
plasma membrane 2

Gene Ontology (GO) - Cellular Components for MED28 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005856 cytoskeleton IEA --
GO:0016592 mediator complex IEA,IBA --
GO:0030864 cortical actin cytoskeleton IEA --
genes like me logo Genes that share ontologies with MED28: view

Pathways & Interactions for MED28 Gene

genes like me logo Genes that share pathways with MED28: view

SIGNOR curated interactions for MED28 Gene

Is activated by:

Gene Ontology (GO) - Biological Process for MED28 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0019827 stem cell population maintenance IEA --
genes like me logo Genes that share ontologies with MED28: view

Drugs & Compounds for MED28 Gene

No Compound Related Data Available

Transcripts for MED28 Gene

Unigene Clusters for MED28 Gene

Mediator complex subunit 28:
Representative Sequences:

Inhibitory RNA Products

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for MED28 Gene

No ASD Table

Relevant External Links for MED28 Gene

GeneLoc Exon Structure for
MED28
ECgene alternative splicing isoforms for
MED28

Expression for MED28 Gene

mRNA expression in normal human tissues for MED28 Gene

Protein differential expression in normal tissues from HIPED for MED28 Gene

This gene is overexpressed in Placenta (15.6), Spleen (14.4), Ovary (13.5), and Fetal ovary (7.7).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MOPED, and MaxQB for MED28 Gene



SOURCE GeneReport for Unigene cluster for MED28 Gene Hs.731966

mRNA Expression by UniProt/SwissProt for MED28 Gene

Q9H204-MED28_HUMAN
Tissue specificity: Widely expressed. Highly expressed in vascular tissues such as placenta, testis and liver.
genes like me logo Genes that share expression patterns with MED28: view

Protein tissue co-expression partners for MED28 Gene

Primer Products

In Situ Assay Products

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery and mRNA differential expression in normal tissues for MED28 Gene

Orthologs for MED28 Gene

This gene was present in the common ancestor of animals.

Orthologs for MED28 Gene

Organism Taxonomy Gene Similarity Type Details
cow
(Bos Taurus)
Mammalia MED28 35
  • 88.76 (n)
  • 93.82 (a)
MED28 36
  • 95 (a)
OneToOne
dog
(Canis familiaris)
Mammalia MED28 35
  • 88.57 (n)
  • 93.14 (a)
MED28 36
  • 93 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Med28 35
  • 87.83 (n)
  • 94.38 (a)
Med28 16
Med28 36
  • 94 (a)
OneToOne
chimpanzee
(Pan troglodytes)
Mammalia MED28 35
  • 99.44 (n)
  • 100 (a)
MED28 36
  • 98 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Med28 35
  • 87.64 (n)
  • 94.94 (a)
oppossum
(Monodelphis domestica)
Mammalia MED28 36
  • 74 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia MED28 36
  • 71 (a)
OneToOne
chicken
(Gallus gallus)
Aves MED28 35
  • 70.83 (n)
  • 80.68 (a)
MED28 36
  • 83 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia MED28 36
  • 89 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia med28 35
  • 73.51 (n)
  • 86.09 (a)
Str.20230 35
zebrafish
(Danio rerio)
Actinopterygii med28 35
  • 74.56 (n)
  • 80.92 (a)
wufc74b11 35
med28 36
  • 77 (a)
OneToOne
rainbow trout
(Oncorhynchus mykiss)
Actinopterygii Omy.13792 35
fruit fly
(Drosophila melanogaster)
Insecta MED28 35
  • 49.87 (n)
  • 40.46 (a)
MED28 36
  • 30 (a)
OneToOne
sea squirt
(Ciona savignyi)
Ascidiacea -- 36
  • 39 (a)
OneToOne
Species with no ortholog for MED28:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for MED28 Gene

ENSEMBL:
Gene Tree for MED28 (if available)
TreeFam:
Gene Tree for MED28 (if available)

Paralogs for MED28 Gene

genes like me logo Genes that share paralogs with MED28: view

No data available for Paralogs for MED28 Gene

Variants for MED28 Gene

Sequence variations from dbSNP and Humsavar for MED28 Gene

SNP ID Clin Chr 04 pos Sequence Context AA Info Type
rs555 -- 17,624,035(+) TTAGC(A/G)AAGTT utr-variant-3-prime
rs1006722 -- 17,617,090(-) GGAAG(C/T)CTTCT intron-variant
rs1006723 -- 17,616,901(-) ACCTA(C/T)AGACA intron-variant
rs964012 -- 17,617,561(-) GTGCT(C/T)GTCCT intron-variant
rs2159584 -- 17,618,945(-) TTTAA(C/T)GAGCA intron-variant

Variation tolerance for MED28 Gene

Residual Variation Intolerance Score: 29.7% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 0.90; 18.65% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for MED28 Gene

HapMap Linkage Disequilibrium report
MED28

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot and Structural Variations from Database of Genomic Variants (DGV) for MED28 Gene

Disorders for MED28 Gene

MalaCards: The human disease database

(2) MalaCards diseases for MED28 Gene - From: DISEASES

Disorder Aliases PubMed IDs
chronic intestinal vascular insufficiency
  • chronic mesenteric ischemia
cholinergic urticaria
- elite association - COSMIC cancer census association via MalaCards
Search MED28 in MalaCards View complete list of genes associated with diseases

Relevant External Links for MED28

Genetic Association Database (GAD)
MED28
Human Genome Epidemiology (HuGE) Navigator
MED28
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
MED28
genes like me logo Genes that share disorders with MED28: view

No data available for UniProtKB/Swiss-Prot and Genatlas for MED28 Gene

Publications for MED28 Gene

  1. Magicin, a novel cytoskeletal protein associates with the NF2 tumor suppressor merlin and Grb2. (PMID: 15467741) Wiederhold T. … Ramesh V. (Oncogene 2004) 2 3 4 23 67
  2. Identification of a novel endothelial-derived gene EG-1. (PMID: 11779215) Liu C. … Nguyen M.H. (Biochem. Biophys. Res. Commun. 2002) 2 3 23
  3. The BioPlex Network: A Systematic Exploration of the Human Interactome. (PMID: 26186194) Huttlin E.L. … Gygi S.P. (Cell 2015) 3
  4. Subunit architecture and functional modular rearrangements of the transcriptional mediator complex. (PMID: 24882805) Tsai K.L. … Asturias F.J. (Cell 2014) 3
  5. Characterization of the influence of mediator complex in HIV-1 transcription. (PMID: 25100719) Ruiz A. … EstAc J.A. (J. Biol. Chem. 2014) 3

Products for MED28 Gene

Sources for MED28 Gene

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