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Aliases for MED27 Gene

Aliases for MED27 Gene

  • Mediator Complex Subunit 27 2 3 4 5
  • Cofactor Required For Sp1 Transcriptional Activation, Subunit 8, 34kDa 2 3
  • Transcriptional Coactivator CRSP34 3 4
  • P37 TRAP/SMCC/PC2 Subunit 3 4
  • CRSP Complex Subunit 8 3 4
  • CRSP34 3 4
  • CRSP8 3 4
  • Cofactor Required For Sp1 Transcriptional Activation Subunit 8 4
  • Mediator Of RNA Polymerase II Transcription Subunit 27 3
  • CRAP34 3
  • TRAP37 3
  • MED3 3

External Ids for MED27 Gene

Previous HGNC Symbols for MED27 Gene

  • CRSP8

Previous GeneCards Identifiers for MED27 Gene

  • GC09M133726
  • GC09M134735
  • GC09M104229

Summaries for MED27 Gene

Entrez Gene Summary for MED27 Gene

  • The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 5. [provided by RefSeq, Dec 2011]

GeneCards Summary for MED27 Gene

MED27 (Mediator Complex Subunit 27) is a Protein Coding gene. Diseases associated with MED27 include Infratentorial Cancer. Among its related pathways are Regulation of lipid metabolism by Peroxisome proliferator-activated receptor alpha (PPARalpha) and Gene Expression. GO annotations related to this gene include transcription coactivator activity.

UniProtKB/Swiss-Prot for MED27 Gene

  • Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Gene Wiki entry for MED27 Gene

No data available for CIViC summary , Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for MED27 Gene

Genomics for MED27 Gene

Regulatory Elements for MED27 Gene

Enhancers for MED27 Gene
GeneHancer Identifier Enhancer Score Enhancer Sources Gene-Enhancer Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Gene Targets for Enhancer
GH09G132019 1.2 ENCODE dbSUPER 31.2 +59.3 59307 2.0 FOXA2 SIN3A FEZF1 ZNF2 GLIS2 FOS DEK YY2 TSHZ1 REST MED27 TTF1 SETX PIR57956
GH09G132041 1.4 Ensembl ENCODE dbSUPER 20.6 +36.9 36930 3.3 SIN3A RAD21 GATA2 FOS HDAC8 ZNF592 NFIC ZNF589 SMARCA4 IKZF1 MED27 ENSG00000176868 POMT1 PIR57956
GH09G132006 1.7 FANTOM5 Ensembl ENCODE dbSUPER 10.3 +71.9 71931 3.9 TBP PKNOX1 FOXA2 TBL1XR1 ZNF133 SIN3A FEZF1 RFX5 GATA2 RCOR1 NTNG2 MED27 SETX PIR57956
GH09G132010 0.8 dbSUPER 21.4 +65.7 65723 6.6 SOX13 FOXA2 ZKSCAN1 FOXK2 FOS MAFK HOMEZ SOX5 GATAD2A RFX1 MED27 NTNG2 ENSG00000246851 PIR57956
GH09G132943 1.2 ENCODE 12.4 -864.6 -864627 2.5 CREB3L1 FEZF1 DMAP1 YY1 ZNF143 ZNF263 SP3 NFYC TBX21 MEF2D TTF1 SETX MED27 TSC1 AK8 GFI1B
- Elite enhancer and/or Elite enhancer-gene association Download GeneHancer data dump

Enhancers around MED27 on UCSC Golden Path with GeneCards custom track

Promoters for MED27 Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters
ENSR00000242434 408 1401 PKNOX1 CREB3L1 ARID4B SIN3A FEZF1 ZNF2 YY1 ZNF766 ZNF143 DEK

Genomic Location for MED27 Gene

Chromosome:
9
Start:
131,860,107 bp from pter
End:
132,079,908 bp from pter
Size:
219,802 bases
Orientation:
Minus strand

Genomic View for MED27 Gene

Genes around MED27 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
MED27 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for MED27 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for MED27 Gene

Proteins for MED27 Gene

  • Protein details for MED27 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q6P2C8-MED27_HUMAN
    Recommended name:
    Mediator of RNA polymerase II transcription subunit 27
    Protein Accession:
    Q6P2C8
    Secondary Accessions:
    • O95401
    • Q4F964
    • Q5VTA4
    • Q5VTA5
    • Q9BU57
    • Q9NYR4
    • V9GYV9

    Protein attributes for MED27 Gene

    Size:
    311 amino acids
    Molecular mass:
    35432 Da
    Quaternary structure:
    • Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP.
    SequenceCaution:
    • Sequence=AAH02878.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305}; Sequence=AAH02878.1; Type=Erroneous termination; Positions=109; Note=Translated as Gln.; Evidence={ECO:0000305};

    Alternative splice isoforms for MED27 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for MED27 Gene

Post-translational modifications for MED27 Gene

  • Ubiquitination at posLast=5050, Lys112, Lys116, posLast=145145, posLast=233233, and posLast=275275
  • Modification sites at PhosphoSitePlus

Other Protein References for MED27 Gene

No data available for DME Specific Peptides for MED27 Gene

Domains & Families for MED27 Gene

Gene Families for MED27 Gene

Protein Domains for MED27 Gene

InterPro:
ProtoNet:

Suggested Antigen Peptide Sequences for MED27 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

Q6P2C8

UniProtKB/Swiss-Prot:

MED27_HUMAN :
  • Belongs to the Mediator complex subunit 27 family.
Family:
  • Belongs to the Mediator complex subunit 27 family.
genes like me logo Genes that share domains with MED27: view

Function for MED27 Gene

Molecular function for MED27 Gene

UniProtKB/Swiss-Prot Function:
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Gene Ontology (GO) - Molecular Function for MED27 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003713 transcription coactivator activity TAS,IDA 9989412
GO:0005515 protein binding IPI 12584197
GO:0061630 ubiquitin protein ligase activity IEA --
genes like me logo Genes that share ontologies with MED27: view
genes like me logo Genes that share phenotypes with MED27: view

Animal Model Products

CRISPR Products

Inhibitory RNA Products

Clone Products

  • Addgene plasmids for MED27

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , Animal Models , miRNA , Transcription Factor Targets and HOMER Transcription for MED27 Gene

Localization for MED27 Gene

Subcellular locations from UniProtKB/Swiss-Prot for MED27 Gene

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for MED27 gene
Compartment Confidence
nucleus 5
cytosol 5
plasma membrane 1
mitochondrion 1
peroxisome 1
endoplasmic reticulum 1

Gene Ontology (GO) - Cellular Components for MED27 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000151 ubiquitin ligase complex IEA --
GO:0005634 nucleus IDA,IEA 9989412
GO:0005654 nucleoplasm TAS --
GO:0005667 transcription factor complex IDA 9989412
GO:0005730 nucleolus IDA --
genes like me logo Genes that share ontologies with MED27: view

Pathways & Interactions for MED27 Gene

genes like me logo Genes that share pathways with MED27: view

Pathways by source for MED27 Gene

Gene Ontology (GO) - Biological Process for MED27 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006351 transcription, DNA-templated IEA --
GO:0006355 regulation of transcription, DNA-templated IEA --
GO:0006357 regulation of transcription from RNA polymerase II promoter IDA 9989412
GO:0006367 transcription initiation from RNA polymerase II promoter TAS --
GO:0016567 protein ubiquitination IEA --
genes like me logo Genes that share ontologies with MED27: view

No data available for SIGNOR curated interactions for MED27 Gene

Drugs & Compounds for MED27 Gene

No Compound Related Data Available

Transcripts for MED27 Gene

Unigene Clusters for MED27 Gene

Mediator complex subunit 27:
Representative Sequences:

CRISPR Products

Inhibitory RNA Products

Clone Products

  • Addgene plasmids for MED27

Alternative Splicing Database (ASD) splice patterns (SP) for MED27 Gene

No ASD Table

Relevant External Links for MED27 Gene

GeneLoc Exon Structure for
MED27
ECgene alternative splicing isoforms for
MED27

Expression for MED27 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and CGAP SAGE for MED27 Gene

Protein differential expression in normal tissues from HIPED for MED27 Gene

This gene is overexpressed in Breast (35.2) and Monocytes (18.6).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MaxQB, and MOPED for MED27 Gene



Protein tissue co-expression partners for MED27 Gene

NURSA nuclear receptor signaling pathways regulating expression of MED27 Gene:

MED27

SOURCE GeneReport for Unigene cluster for MED27 Gene:

Hs.374262

Evidence on tissue expression from TISSUES for MED27 Gene

  • Nervous system(3.1)
genes like me logo Genes that share expression patterns with MED27: view

Primer Products

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , mRNA differential expression in normal tissues , mRNA Expression by UniProt/SwissProt and Phenotype-based relationships between genes and organs from Gene ORGANizer for MED27 Gene

Orthologs for MED27 Gene

This gene was present in the common ancestor of animals.

Orthologs for MED27 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia MED27 35
  • 100 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia MED27 35
  • 99 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia MED27 35
  • 97 (a)
OneToOne
dog
(Canis familiaris)
Mammalia MED27 34 35
  • 94.53 (n)
cow
(Bos Taurus)
Mammalia MED27 34 35
  • 91.1 (n)
mouse
(Mus musculus)
Mammalia Med27 34 16 35
  • 88.21 (n)
rat
(Rattus norvegicus)
Mammalia Med27 34
  • 88.1 (n)
chicken
(Gallus gallus)
Aves MED27 34 35
  • 84.31 (n)
lizard
(Anolis carolinensis)
Reptilia MED27 35
  • 87 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia med27 34
  • 80.49 (n)
Str.899 34
African clawed frog
(Xenopus laevis)
Amphibia Xl.23148 34
zebrafish
(Danio rerio)
Actinopterygii med27 34 35
  • 75.78 (n)
zgc66302 34
fruit fly
(Drosophila melanogaster)
Insecta MED27 34 35
  • 50.74 (n)
African malaria mosquito
(Anopheles gambiae)
Insecta MED27_ANOGA 34
  • 50.4 (n)
sea squirt
(Ciona savignyi)
Ascidiacea -- 35
  • 38 (a)
OneToOne
Species where no ortholog for MED27 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for MED27 Gene

ENSEMBL:
Gene Tree for MED27 (if available)
TreeFam:
Gene Tree for MED27 (if available)

Paralogs for MED27 Gene

Pseudogenes.org Pseudogenes for MED27 Gene

genes like me logo Genes that share paralogs with MED27: view

No data available for Paralogs for MED27 Gene

Variants for MED27 Gene

Sequence variations from dbSNP and Humsavar for MED27 Gene

SNP ID Clin Chr 09 pos Sequence Context AA Info Type
rs1000006118 -- 132,062,830(+) GTTGC(C/T)GAGAT intron-variant
rs1000006636 -- 131,950,942(+) TTTCT(A/T)AATCA intron-variant
rs1000006680 -- 132,040,489(+) CTGCC(C/T)GTACA intron-variant
rs1000039865 -- 131,995,953(+) ATTCC(C/T)GATAT intron-variant
rs1000077467 -- 131,996,203(+) GCTGT(C/T)ACACA intron-variant

Structural Variations from Database of Genomic Variants (DGV) for MED27 Gene

Variant ID Type Subtype PubMed ID
esv2481552 CNV deletion 19546169
esv2619384 CNV insertion 19546169
esv2659649 CNV deletion 23128226
esv2662096 CNV deletion 23128226
esv2677413 CNV deletion 23128226
esv3402046 CNV duplication 20981092
esv3432278 CNV duplication 20981092
esv3444132 CNV duplication 20981092
esv3573403 CNV loss 25503493
esv3573404 CNV loss 25503493
esv3621896 CNV gain 21293372
esv3621898 CNV gain 21293372
esv3621902 CNV gain 21293372
esv3621904 CNV loss 21293372
esv3621906 CNV loss 21293372
esv3621907 CNV loss 21293372
esv3621908 CNV loss 21293372
esv3621909 CNV loss 21293372
nsv1038424 CNV gain 25217958
nsv1051484 CNV loss 25217958
nsv512994 CNV insertion 21212237

Variation tolerance for MED27 Gene

Residual Variation Intolerance Score: 35.6% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 2.17; 39.38% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for MED27 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
MED27

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for MED27 Gene

Disorders for MED27 Gene

MalaCards: The human disease database

(1) MalaCards diseases for MED27 Gene - From: DISEASES and GeneCards

Disorder Aliases PubMed IDs
infratentorial cancer
  • brain neoplasm, infratentorial
- elite association - COSMIC cancer census association via MalaCards
Search MED27 in MalaCards View complete list of genes associated with diseases

Relevant External Links for MED27

Genetic Association Database (GAD)
MED27
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
MED27
genes like me logo Genes that share disorders with MED27: view

No data available for UniProtKB/Swiss-Prot and Genatlas for MED27 Gene

Publications for MED27 Gene

  1. The transcriptional cofactor complex CRSP is required for activity of the enhancer-binding protein Sp1. (PMID: 9989412) Ryu S. … Tjian R. (Nature 1999) 2 3 4 64
  2. MED1/TRAP220 exists predominantly in a TRAP/Mediator subpopulation enriched in RNA polymerase II and is required for ER-mediated transcription. (PMID: 15989967) Zhang X. … Roeder R.G. (Mol. Cell 2005) 3 4 64
  3. DNA sequence and analysis of human chromosome 9. (PMID: 15164053) Humphray S.J. … Dunham I. (Nature 2004) 3 4 64
  4. A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology. (PMID: 15175163) Sato S. … Conaway R.C. (Mol. Cell 2004) 3 4 64
  5. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PMID: 15489334) Gerhard D.S. … Malek J. (Genome Res. 2004) 3 4 64

Products for MED27 Gene

  • Addgene plasmids for MED27

Sources for MED27 Gene

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