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Aliases for MED27 Gene

Aliases for MED27 Gene

  • Mediator Complex Subunit 27 2 3 4 5
  • Cofactor Required For Sp1 Transcriptional Activation, Subunit 8, 34kDa 2 3
  • Transcriptional Coactivator CRSP34 3 4
  • P37 TRAP/SMCC/PC2 Subunit 3 4
  • CRSP Complex Subunit 8 3 4
  • CRSP34 3 4
  • CRSP8 3 4
  • Cofactor Required For Sp1 Transcriptional Activation Subunit 8 4
  • CRAP34 3
  • TRAP37 3
  • MED3 3

External Ids for MED27 Gene

Previous HGNC Symbols for MED27 Gene

  • CRSP8

Previous GeneCards Identifiers for MED27 Gene

  • GC09M133726
  • GC09M134735
  • GC09M104229

Summaries for MED27 Gene

Entrez Gene Summary for MED27 Gene

  • The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 5. [provided by RefSeq, Dec 2011]

GeneCards Summary for MED27 Gene

MED27 (Mediator Complex Subunit 27) is a Protein Coding gene. Among its related pathways are Gene Expression and Metabolism. GO annotations related to this gene include transcription coactivator activity.

UniProtKB/Swiss-Prot for MED27 Gene

  • Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Gene Wiki entry for MED27 Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for MED27 Gene

Genomics for MED27 Gene

Regulatory Elements for MED27 Gene

Promoters for MED27 Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters

ENSRs around MED27 on UCSC Golden Path with GeneCards custom track

Genomic Location for MED27 Gene

Chromosome:
9
Start:
131,860,107 bp from pter
End:
132,079,908 bp from pter
Size:
219,802 bases
Orientation:
Minus strand

Genomic View for MED27 Gene

Genes around MED27 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
MED27 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for MED27 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for MED27 Gene

Proteins for MED27 Gene

  • Protein details for MED27 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q6P2C8-MED27_HUMAN
    Recommended name:
    Mediator of RNA polymerase II transcription subunit 27
    Protein Accession:
    Q6P2C8
    Secondary Accessions:
    • O95401
    • Q4F964
    • Q5VTA4
    • Q5VTA5
    • Q9BU57
    • Q9NYR4
    • V9GYV9

    Protein attributes for MED27 Gene

    Size:
    311 amino acids
    Molecular mass:
    35432 Da
    Quaternary structure:
    • Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP.
    SequenceCaution:
    • Sequence=AAH02878.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305}; Sequence=AAH02878.1; Type=Erroneous termination; Positions=109; Note=Translated as Gln.; Evidence={ECO:0000305};

    Alternative splice isoforms for MED27 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for MED27 Gene

Proteomics data for MED27 Gene at MOPED

Post-translational modifications for MED27 Gene

  • Ubiquitination at Lys 50, Lys 112, Lys 116, Lys 145, Lys 233, and Lys 275
  • Modification sites at PhosphoSitePlus

Other Protein References for MED27 Gene

No data available for DME Specific Peptides for MED27 Gene

Domains & Families for MED27 Gene

Gene Families for MED27 Gene

Protein Domains for MED27 Gene

InterPro:
ProtoNet:

Suggested Antigen Peptide Sequences for MED27 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

Q6P2C8

UniProtKB/Swiss-Prot:

MED27_HUMAN :
  • Belongs to the Mediator complex subunit 27 family.
Family:
  • Belongs to the Mediator complex subunit 27 family.
genes like me logo Genes that share domains with MED27: view

Function for MED27 Gene

Molecular function for MED27 Gene

UniProtKB/Swiss-Prot Function:
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
genes like me logo Genes that share phenotypes with MED27: view

Animal Model Products

CRISPR Products

No data available for Enzyme Numbers (IUBMB) , Gene Ontology (GO) - Molecular Function , Human Phenotype Ontology , Animal Models , miRNA , Transcription Factor Targets and HOMER Transcription for MED27 Gene

Localization for MED27 Gene

Subcellular locations from UniProtKB/Swiss-Prot for MED27 Gene

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for MED27 Gene COMPARTMENTS Subcellular localization image for MED27 gene
Compartment Confidence
nucleus 5
cytosol 3
endoplasmic reticulum 1
mitochondrion 1
peroxisome 1
plasma membrane 1

Gene Ontology (GO) - Cellular Components for MED27 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005654 nucleoplasm TAS --
GO:0005667 transcription factor complex IDA 9989412
GO:0005737 cytoplasm IDA --
GO:0016592 mediator complex IEA --
genes like me logo Genes that share ontologies with MED27: view

Pathways & Interactions for MED27 Gene

genes like me logo Genes that share pathways with MED27: view

Gene Ontology (GO) - Biological Process for MED27 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006367 transcription initiation from RNA polymerase II promoter TAS --
GO:0019827 stem cell population maintenance IEA --
genes like me logo Genes that share ontologies with MED27: view

No data available for SIGNOR curated interactions for MED27 Gene

Drugs & Compounds for MED27 Gene

No Compound Related Data Available

Transcripts for MED27 Gene

Unigene Clusters for MED27 Gene

Mediator complex subunit 27:
Representative Sequences:

CRISPR Products

Inhibitory RNA Products

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for MED27 Gene

No ASD Table

Relevant External Links for MED27 Gene

GeneLoc Exon Structure for
MED27
ECgene alternative splicing isoforms for
MED27

Expression for MED27 Gene

mRNA expression in normal human tissues for MED27 Gene

Protein differential expression in normal tissues from HIPED for MED27 Gene

This gene is overexpressed in Breast (35.2) and Monocytes (18.6).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MOPED, and MaxQB for MED27 Gene



SOURCE GeneReport for Unigene cluster for MED27 Gene Hs.374262

genes like me logo Genes that share expression patterns with MED27: view

Protein tissue co-expression partners for MED27 Gene

Primer Products

In Situ Assay Products

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , mRNA differential expression in normal tissues and mRNA Expression by UniProt/SwissProt for MED27 Gene

Orthologs for MED27 Gene

This gene was present in the common ancestor of animals.

Orthologs for MED27 Gene

Organism Taxonomy Gene Similarity Type Details
cow
(Bos Taurus)
Mammalia MED27 35
  • 91.1 (n)
  • 98.39 (a)
MED27 36
  • 98 (a)
OneToOne
dog
(Canis familiaris)
Mammalia MED27 35
  • 94.53 (n)
  • 98.07 (a)
MED27 36
  • 98 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Med27 35
  • 88.1 (n)
  • 98.07 (a)
mouse
(Mus musculus)
Mammalia Med27 16
Med27 36
  • 98 (a)
OneToOne
Med27 35
  • 88.21 (n)
  • 98.07 (a)
oppossum
(Monodelphis domestica)
Mammalia MED27 36
  • 97 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia MED27 36
  • 99 (a)
OneToOne
chimpanzee
(Pan troglodytes)
Mammalia MED27 36
  • 100 (a)
OneToOne
chicken
(Gallus gallus)
Aves MED27 35
  • 84.31 (n)
  • 94.81 (a)
MED27 36
  • 94 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia MED27 36
  • 87 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia med27 35
  • 80.49 (n)
  • 92.28 (a)
Str.899 35
African clawed frog
(Xenopus laevis)
Amphibia Xl.23148 35
zebrafish
(Danio rerio)
Actinopterygii med27 35
  • 75.78 (n)
  • 88.42 (a)
zgc66302 35
med27 36
  • 88 (a)
OneToOne
African malaria mosquito
(Anopheles gambiae)
Insecta MED27_ANOGA 35
  • 50.4 (n)
  • 43.05 (a)
fruit fly
(Drosophila melanogaster)
Insecta MED27 35
  • 50.74 (n)
  • 39.86 (a)
MED27 36
  • 39 (a)
OneToOne
sea squirt
(Ciona savignyi)
Ascidiacea -- 36
  • 38 (a)
OneToOne
Species with no ortholog for MED27:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for MED27 Gene

ENSEMBL:
Gene Tree for MED27 (if available)
TreeFam:
Gene Tree for MED27 (if available)

Paralogs for MED27 Gene

Pseudogenes.org Pseudogenes for MED27 Gene

genes like me logo Genes that share paralogs with MED27: view

No data available for Paralogs for MED27 Gene

Variants for MED27 Gene

Sequence variations from dbSNP and Humsavar for MED27 Gene

SNP ID Clin Chr 09 pos Sequence Context AA Info Type
rs913509 -- 131,862,364(+) CTGGC(A/G)TGAGA intron-variant
rs2281998 -- 131,860,193(+) GACGA(C/T)ACCCA utr-variant-3-prime
rs4128956 -- 131,943,122(-) TTTGT(A/G)TTAGG intron-variant
rs4128957 -- 131,943,031(-) CGGGT(C/T)GGGGA intron-variant
rs2987405 -- 131,862,715(+) ACGAT(C/T)TGTTA intron-variant

Structural Variations from Database of Genomic Variants (DGV) for MED27 Gene

Variant ID Type Subtype PubMed ID
nsv894012 CNV Loss 21882294
esv2677413 CNV Deletion 23128226
esv2659649 CNV Deletion 23128226
nsv512994 CNV Insertion 21212237
esv2619384 CNV Insertion 19546169
nsv894013 CNV Gain 21882294
esv2481552 CNV Deletion 19546169
esv2662096 CNV Deletion 23128226

Variation tolerance for MED27 Gene

Residual Variation Intolerance Score: 35.6% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 2.17; 39.38% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for MED27 Gene

HapMap Linkage Disequilibrium report
MED27

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for MED27 Gene

Disorders for MED27 Gene

Relevant External Links for MED27

Genetic Association Database (GAD)
MED27
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
MED27

No disorders were found for MED27 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for MED27 Gene

Publications for MED27 Gene

  1. The transcriptional cofactor complex CRSP is required for activity of the enhancer-binding protein Sp1. (PMID: 9989412) Ryu S. … Tjian R. (Nature 1999) 2 3 4 67
  2. The BioPlex Network: A Systematic Exploration of the Human Interactome. (PMID: 26186194) Huttlin E.L. … Gygi S.P. (Cell 2015) 3
  3. Panorama of ancient metazoan macromolecular complexes. (PMID: 26344197) Wan C. … Emili A. (Nature 2015) 3
  4. Subunit architecture and functional modular rearrangements of the transcriptional mediator complex. (PMID: 24882805) Tsai K.L. … Asturias F.J. (Cell 2014) 3
  5. Characterization of the influence of mediator complex in HIV-1 transcription. (PMID: 25100719) Ruiz A. … EstAc J.A. (J. Biol. Chem. 2014) 3

Products for MED27 Gene

Sources for MED27 Gene

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