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Aliases for MED20 Gene

Aliases for MED20 Gene

  • Mediator Complex Subunit 20 2 3 4 5
  • TRFP 3 4
  • Trf (TATA Binding Protein-Related Factor)-Proximal Homolog (Drosophila) 2
  • Trf (TATA Binding Protein-Related Factor)-Proximal Homolog 3
  • Mediator Of RNA Polymerase II Transcription Subunit 20 3
  • TRF-Proximal Protein Homolog 4
  • PRO0213 3
  • HTRFP 4

External Ids for MED20 Gene

Previous HGNC Symbols for MED20 Gene

  • TRFP

Previous GeneCards Identifiers for MED20 Gene

  • GC06M041983
  • GC06M041591

Summaries for MED20 Gene

Entrez Gene Summary for MED20 Gene

  • This gene encodes a component of the mediator complex (also known as TRAP, SMCC, DRIP, or ARC), a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. A mutation in this gene has been associated with a novel infantile-onset neurodegenerative movement disorder. Alternatively spliced transcript variants have been identified. [provided by RefSeq, Mar 2015]

GeneCards Summary for MED20 Gene

MED20 (Mediator Complex Subunit 20) is a Protein Coding gene. Among its related pathways are Regulation of lipid metabolism by Peroxisome proliferator-activated receptor alpha (PPARalpha) and Metabolism. GO annotations related to this gene include DNA-directed RNA polymerase activity and RNA polymerase II transcription cofactor activity.

UniProtKB/Swiss-Prot for MED20 Gene

  • Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

No data available for Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for MED20 Gene

Genomics for MED20 Gene

Regulatory Elements for MED20 Gene

Enhancers for MED20 Gene
GeneHancer Identifier Enhancer Score Enhancer Sources Gene-Enhancer Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Gene Targets for Enhancer
GH06F041784 0.7 ENCODE 33 +134.9 134921 4.2 HDGF PKNOX1 ARNT CREB3L1 ZFP64 ARID4B SIN3A ZNF2 YY1 SLC30A9 PRICKLE4 MED20 FRS3 USP49 BYSL ENSG00000207371 PGC
GH06F041878 0.2 ENCODE 28.6 +42.0 41977 1.3 ATF1 PKNOX1 ARNT SIN3A ARID4B DMAP1 SLC30A9 GATA2 FOS DEK PRICKLE4 MED20 BYSL ENSG00000207371 ENSG00000206977 RNU6-761P ENSG00000227516 USP49
GH06F041947 0.3 FANTOM5 28.4 -26.3 -26328 0.4 KLF1 CEBPB ZBTB33 MED20 RNU6-761P ENSG00000207371 BYSL USP49 ENSG00000206977 LOC105375060
GH06F041845 0.8 Ensembl ENCODE 28.3 +74.7 74663 2.0 PKNOX1 ATF1 SIN3A ARID4B DMAP1 SLC30A9 GATA2 ZNF143 FOS ZNF202 PRICKLE4 MED20 USP49 CCND3 TAF8 BYSL ENSG00000207371 RNU6-761P ENSG00000206977 ENSG00000227516
GH06F041956 0.4 ENCODE 25 -36.2 -36169 1.3 HDAC1 ARNT TBL1XR1 TAL1 GATA2 NCOR1 STAT2 NR2F6 TRIM24 SMARCE1 PRICKLE4 MED20 TREML2 RNU6-761P ENSG00000207371 BYSL ENSG00000206977 LOC105375060
- Elite enhancer/Elite enhancer-gene association Download Table
Download GeneHancer data dump

Enhancers around MED20 on UCSC Golden Path with GeneCards custom track

Promoters for MED20 Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters
ENSR00001495310 -361 2601 PKNOX1 CREB3L1 ARID4B SIN3A DMAP1 ZNF2 YY1 ZNF207 ZNF143 KLF13

Genomic Location for MED20 Gene

Chromosome:
6
Start:
41,905,354 bp from pter
End:
41,921,139 bp from pter
Size:
15,786 bases
Orientation:
Minus strand

Genomic View for MED20 Gene

Genes around MED20 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
MED20 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for MED20 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for MED20 Gene

Proteins for MED20 Gene

  • Protein details for MED20 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q9H944-MED20_HUMAN
    Recommended name:
    Mediator of RNA polymerase II transcription subunit 20
    Protein Accession:
    Q9H944
    Secondary Accessions:
    • B4DE08
    • O95821
    • Q5T8J4
    • Q9Y429

    Protein attributes for MED20 Gene

    Size:
    212 amino acids
    Molecular mass:
    23222 Da
    Quaternary structure:
    • Interacts with PPARG (By similarity). Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP.

    Alternative splice isoforms for MED20 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for MED20 Gene

Post-translational modifications for MED20 Gene

  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

No data available for DME Specific Peptides for MED20 Gene

Domains & Families for MED20 Gene

Gene Families for MED20 Gene

Protein Domains for MED20 Gene

Graphical View of Domain Structure for InterPro Entry

Q9H944

UniProtKB/Swiss-Prot:

MED20_HUMAN :
  • Belongs to the Mediator complex subunit 20 family.
Family:
  • Belongs to the Mediator complex subunit 20 family.
genes like me logo Genes that share domains with MED20: view

Function for MED20 Gene

Molecular function for MED20 Gene

UniProtKB/Swiss-Prot Function:
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Gene Ontology (GO) - Molecular Function for MED20 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0001104 RNA polymerase II transcription cofactor activity IEA --
GO:0003713 transcription coactivator activity IBA --
GO:0003899 DNA-directed RNA polymerase activity NAS 9933582
GO:0005515 protein binding IPI 14576168
GO:0061630 ubiquitin protein ligase activity IEA --
genes like me logo Genes that share ontologies with MED20: view
genes like me logo Genes that share phenotypes with MED20: view

Animal Model Products

miRNA for MED20 Gene

miRTarBase miRNAs that target MED20

Inhibitory RNA Products

Flow Cytometry Products

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for MED20 Gene

Localization for MED20 Gene

Subcellular locations from UniProtKB/Swiss-Prot for MED20 Gene

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for MED20 Gene COMPARTMENTS Subcellular localization image for MED20 gene
Compartment Confidence
nucleus 5
cytoskeleton 2
extracellular 2
cytosol 1
mitochondrion 1

Gene Ontology (GO) - Cellular Components for MED20 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000151 ubiquitin ligase complex IEA --
GO:0005634 nucleus IEA --
GO:0005654 nucleoplasm TAS --
GO:0016592 mediator complex IEA,NAS 9933582
genes like me logo Genes that share ontologies with MED20: view

Pathways & Interactions for MED20 Gene

genes like me logo Genes that share pathways with MED20: view

Gene Ontology (GO) - Biological Process for MED20 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006351 transcription, DNA-templated NAS 9933582
GO:0006355 regulation of transcription, DNA-templated IEA --
GO:0006357 regulation of transcription from RNA polymerase II promoter IEA --
GO:0006366 transcription from RNA polymerase II promoter TAS 9933582
GO:0006367 transcription initiation from RNA polymerase II promoter TAS --
genes like me logo Genes that share ontologies with MED20: view

No data available for SIGNOR curated interactions for MED20 Gene

Transcripts for MED20 Gene

Unigene Clusters for MED20 Gene

Mediator complex subunit 20:
Representative Sequences:

Inhibitory RNA Products

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for MED20 Gene

ExUns: 1a · 1b · 1c ^ 2 ^ 3 ^ 4 ^ 5 ^ 6a · 6b ^ 7a · 7b · 7c · 7d
SP1: - -
SP2: - - - -
SP3: - - - - -
SP4: - - - - -
SP5: - - -
SP6: -

Relevant External Links for MED20 Gene

GeneLoc Exon Structure for
MED20
ECgene alternative splicing isoforms for
MED20

Expression for MED20 Gene

mRNA expression in normal human tissues for MED20 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Protein differential expression in normal tissues from HIPED for MED20 Gene

This gene is overexpressed in Bone (16.0).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MaxQB, and MOPED for MED20 Gene



Protein tissue co-expression partners for MED20 Gene

- Elite partner

NURSA nuclear receptor signaling pathways regulating expression of MED20 Gene:

MED20

SOURCE GeneReport for Unigene cluster for MED20 Gene:

Hs.278434
genes like me logo Genes that share expression patterns with MED20: view

Primer Products

No data available for mRNA differential expression in normal tissues and mRNA Expression by UniProt/SwissProt for MED20 Gene

Orthologs for MED20 Gene

This gene was present in the common ancestor of animals.

Orthologs for MED20 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia MED20 34 35
  • 99.69 (n)
oppossum
(Monodelphis domestica)
Mammalia MED20 35
  • 94 (a)
OneToOne
dog
(Canis familiaris)
Mammalia MED20 34 35
  • 92.92 (n)
cow
(Bos Taurus)
Mammalia MED20 34 35
  • 92.61 (n)
rat
(Rattus norvegicus)
Mammalia Med20 34
  • 91.67 (n)
mouse
(Mus musculus)
Mammalia Med20 34 16 35
  • 91.19 (n)
Gm20517 35
  • 91 (a)
OneToMany
platypus
(Ornithorhynchus anatinus)
Mammalia MED20 35
  • 80 (a)
OneToOne
chicken
(Gallus gallus)
Aves MED20 34 35
  • 78.2 (n)
lizard
(Anolis carolinensis)
Reptilia -- 35
  • 85 (a)
OneToMany
-- 35
  • 80 (a)
OneToMany
tropical clawed frog
(Silurana tropicalis)
Amphibia med20 34
  • 73.78 (n)
Str.17211 34
African clawed frog
(Xenopus laevis)
Amphibia Xl.15205 34
zebrafish
(Danio rerio)
Actinopterygii med20 34 35
  • 66.35 (n)
Dr.23717 34
fruit fly
(Drosophila melanogaster)
Insecta MED20 34 35
  • 51.41 (n)
African malaria mosquito
(Anopheles gambiae)
Insecta MED20_ANOGA 34
  • 50.49 (n)
worm
(Caenorhabditis elegans)
Secernentea mdt-20 34 35
  • 47.7 (n)
Species where no ortholog for MED20 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea squirt (Ciona intestinalis)
  • sea squirt (Ciona savignyi)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for MED20 Gene

ENSEMBL:
Gene Tree for MED20 (if available)
TreeFam:
Gene Tree for MED20 (if available)

Paralogs for MED20 Gene

(1) SIMAP similar genes for MED20 Gene using alignment to 5 proteins:

genes like me logo Genes that share paralogs with MED20: view

No data available for Paralogs for MED20 Gene

Variants for MED20 Gene

Sequence variations from dbSNP and Humsavar for MED20 Gene

SNP ID Clin Chr 06 pos Sequence Context AA Info Type
rs730882147 Uncertain significance 41,909,351(-) CCGGG(C/G)CACCA intron-variant, reference, missense
rs10947993 -- 41,912,487(+) GAGTA(A/G)CTGGG intron-variant
rs10947994 -- 41,914,211(+) ggcag(G/T)aatga intron-variant
rs10947995 -- 41,921,485(+) CGCTG(A/G)GAGTC upstream-variant-2KB, utr-variant-5-prime
rs111352238 -- 41,912,593(+) CTCAG(A/G)TGATC intron-variant

Structural Variations from Database of Genomic Variants (DGV) for MED20 Gene

Variant ID Type Subtype PubMed ID
nsv5278 CNV insertion 18451855
nsv830646 CNV loss 17160897

Variation tolerance for MED20 Gene

Residual Variation Intolerance Score: 34.7% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 0.19; 4.32% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for MED20 Gene

Human Gene Mutation Database (HGMD)
MED20
SNPedia medical, phenotypic, and genealogical associations of SNPs for
MED20

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for MED20 Gene

Disorders for MED20 Gene

Relevant External Links for MED20

Genetic Association Database (GAD)
MED20
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
MED20

No disorders were found for MED20 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for MED20 Gene

Publications for MED20 Gene

  1. A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology. (PMID: 15175163) Sato S. … Conaway R.C. (Mol. Cell 2004) 2 3 4 64
  2. The human homologue of Drosophila TRF-proximal protein is associated with an RNA polymerase II-SRB complex. (PMID: 9933582) Xiao H. … Roeder R.G. (J. Biol. Chem. 1999) 2 3 4 64
  3. MED1/TRAP220 exists predominantly in a TRAP/Mediator subpopulation enriched in RNA polymerase II and is required for ER-mediated transcription. (PMID: 15989967) Zhang X. … Roeder R.G. (Mol. Cell 2005) 3 4 64
  4. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PMID: 14702039) Ota T. … Sugano S. (Nat. Genet. 2004) 3 4 64
  5. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PMID: 15489334) Gerhard D.S. … Malek J. (Genome Res. 2004) 3 4 64

Products for MED20 Gene

Sources for MED20 Gene

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