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MED16 Gene

protein-coding   GIFtS: 55
GCID: GC19M000906

Mediator Complex Subunit 16

(Previous name: thyroid hormone receptor associated protein 5)
(Previous symbol: THRAP5)
  Search for MED16
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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Aliases
Mediator Complex Subunit 161 2 3     Thyroid Hormone Receptor Associated Protein 51
THRAP51 2 3     TRAP952
Thyroid Hormone Receptor-Associated Protein 52 3     Mediator Of RNA Polymerase II Transcription Subunit 162
Thyroid Hormone Receptor-Associated Protein Complex 95 KDa Component2 3     Thyroid Hormone Receptor-Associated Protein, 95-KD Subunit2
Vitamin D3 Receptor-Interacting Protein Complex 92 KDa Component2 3     Trap953
DRIP922 3     

External Ids:    HGNC: 175561   Entrez Gene: 100252   Ensembl: ENSG000001752217   OMIM: 6040625   UniProtKB: Q9Y2X03   

Export aliases for MED16 gene to outside databases

Previous GC identifers: GC19M000819 GC19M000640 GC19M000884 GC19M000889


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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GeneCards Summary for MED16 Gene:
MED16 (mediator complex subunit 16) is a protein-coding gene. GO annotations related to this gene include transcription coactivator activity and transcription cofactor activity.

UniProtKB/Swiss-Prot: MED16_HUMAN, Q9Y2X0
Function: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all
RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific
regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by
direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional
preinitiation complex with RNA polymerase II and the general transcription factors

Gene Wiki entry for MED16 Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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RefSeq DNA sequence at NCBI GenBank:
NC_000019.9  NT_011295.12  NC_018930.2  NT_187622.1  
Regulatory elements:
   Regulatory transcription factor binding sites in the MED16 gene promoter:
         TBP   NF-1/L   NF-1   MIF-1   AP-2gamma   E47   AP-2beta   AP-2alpha   ATF6   AP-2alphaA   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidMED16 promoter sequence
   Search Chromatin IP Primers for MED16

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat MED16


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 19p13.3   Ensembl cytogenetic band:  19p13.3   HGNC cytogenetic band: 19p13.3

MED16 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
MED16 gene location

GeneLoc information about chromosome 19         GeneLoc Exon Structure

GeneLoc location for GC19M000906:  view genomic region     (about GC identifiers)

Start:
867,962 bp from pter      End:
893,218 bp from pter
Size:
25,257 bases      Orientation:
minus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, Cloud-Clone Corp., and/or eBioscience,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, Cloud-Clone Corp., and/or eBioscience, Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, Cloud-Clone Corp, and/or others.)
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UniProtKB/Swiss-Prot: MED16_HUMAN, Q9Y2X0 (See protein sequence)
Recommended Name: Mediator of RNA polymerase II transcription subunit 16  
Size: 877 amino acids; 96793 Da
Subunit: Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11,
MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26,
MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct
module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this
module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or
more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP
Caution: It is uncertain whether Met-1 or Met-13 is the initiator
Sequence caution: Sequence=AAD31087.1; Type=Frameshift; Positions=609;
Secondary accessions: Q6PJT2 Q96AD4 Q96I35 Q9Y652
Alternative splicing: 5 isoforms:  Q9Y2X0-1   Q9Y2X0-2   Q9Y2X0-3   Q9Y2X0-4   Q9Y2X0-5   (No experimental confirmation available)

Explore the universe of human proteins at neXtProt for MED16: NX_Q9Y2X0

Explore proteomics data for MED16 at MOPED

Post-translational modifications: 

  • Ubiquitination2 at Lys26, Lys149, Lys158, Lys252, Lys284, Lys338, Lys574, Lys745, Lys808, Lys817
  • Modification sites at PhosphoSitePlus

  • See MED16 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins: NP_005472.2  
    ENSEMBL proteins: 
     ENSP00000379153   ENSP00000475725   ENSP00000464810   ENSP00000475528   ENSP00000308528  
     ENSP00000466868   ENSP00000465762   ENSP00000465823   ENSP00000465827   ENSP00000325612  
     ENSP00000269814  
    Reactome Protein details: Q9Y2X0

    MED16 Human Recombinant Protein Products:

    Browse Purified and Recombinant Proteins at EMD Millipore
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    Browse recombinant and purified proteins available from Enzo Life Sciences
    Browse OriGene full length recombinant human proteins expressed in human HEK293 cells
    Browse OriGene Protein Over-expression Lysates
    OriGene Custom MassSpec
    OriGene Custom Protein Services for MED16
    GenScript Custom Purified and Recombinant Proteins Services for MED16
    Novus Biologicals MED16 Proteins
    Browse Sino Biological Recombinant Proteins
    Browse Sino Biological Cell Lysates
    Browse ProSpec Recombinant Proteins
    Cloud-Clone Corp. Proteins for MED16

     
    Search eBioscience for Proteins for MED16 

    MED16 Antibody Products:

    Browse EMD Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Browse R&D Systems for Antibodies
    Browse OriGene Antibodies
    OriGene Custom Antibody Services for MED16
    Novus Biologicals MED16 Antibodies
    Abcam antibodies for MED16
    Cloud-Clone Corp. Antibodies for MED16
    ThermoFisher Antibodies for MED16
    LSBio Antibodies in human, mouse, rat for MED16

    MED16 Assay Products:

    Browse Kits and Assays available from EMD Millipore
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    Browse R&D Systems for biochemical assays
    GenScript Custom Assay Services for MED16
    Browse Enzo Life Sciences for kits & assays
    Cloud-Clone Corp. ELISAs for MED16
    Cloud-Clone Corp. CLIAs for MED16
    Search eBioscience for ELISAs for MED16 


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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    HGNC Gene Families:
    WDR: WD repeat domain containing

    5 InterPro protein domains:
     IPR011041 Quinoprot_gluc/sorb_DH
     IPR001680 WD40_repeat
     IPR017986 WD40_repeat_dom
     IPR021665 Mediator_Med16
     IPR015943 WD40/YVTN_repeat-like_dom

    Graphical View of Domain Structure for InterPro Entry Q9Y2X0

    ProtoNet protein and cluster: Q9Y2X0

    UniProtKB/Swiss-Prot: MED16_HUMAN, Q9Y2X0
    Similarity: Belongs to the Mediator complex subunit 16 family
    Similarity: Contains 5 WD repeats


    Find genes that share domains with MED16           About GenesLikeMe


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, Vector BioLabs and/or Addgene, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Flow cytometry from eBioscience, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Molecular Function:

         UniProtKB/Swiss-Prot Summary: MED16_HUMAN, Q9Y2X0
    Function: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all
    RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific
    regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by
    direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional
    preinitiation complex with RNA polymerase II and the general transcription factors

         Gene Ontology (GO): Selected molecular function terms (see all 8):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003712transcription cofactor activity IDA12218053
    GO:0003713transcription coactivator activity TAS10198638
    GO:0003824catalytic activity IEA--
    GO:0004872receptor activity IDA12218053
    GO:0005515protein binding ----
         
    Find genes that share ontologies with MED16           About GenesLikeMe


    Phenotypes:
         1 MGI phenotypic allele for Med16 (no phenotypes)

    Find genes that share phenotypes with MED16           About GenesLikeMe

    Animal Models:
       inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for MED16
       inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for MED16

       genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for MED16
       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for MED16

    miRNA
    Products:
        
    miRTarBase miRNAs that target MED16:
    hsa-mir-197-3p (MIRT004184), hsa-mir-92a-3p (MIRT049666), hsa-mir-346 (MIRT004240), hsa-mir-24-3p (MIRT030487)

    Block miRNA regulation of human, mouse, rat MED16 using miScript Target Protectors
    Search for qRT-PCR Assays for microRNAs that regulate MED16
    SwitchGear 3'UTR luciferase reporter plasmidMED16 3' UTR sequence
    Inhib. RNA
    Products:
        
    OriGene RNAi products in human, mouse, rat for MED16
    Predesigned siRNA for gene silencing in human, mouse, rat MED16

    Gene Editing
    Products:
    DNA2.0 Custom Protein Engineering Service for MED16

    Clone
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    OriGene clones in human, mouse for MED16 (see all 7)
    OriGene ORF clones in mouse, rat for MED16
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector: MED16 (NM_005481)
    Browse Sino Biological Human cDNA Clones
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for MED16
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat MED16

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    Browse ESI BIO Cell Lines and PureStem Progenitors for MED16 
    In Situ Assay
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    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for MED16


    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Subcellular locations from UniProtKB/Swiss-Prot
    MED16_HUMAN, Q9Y2X0: Nucleus (Probable)
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    nucleus5
    cytosol1
    plasma membrane1

    Gene Ontology (GO): 3 cellular component terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634nucleus NAS10198638
    GO:0005654nucleoplasm TAS--
    GO:0016592mediator complex IDA10198638

    Find genes that share ontologies with MED16           About GenesLikeMe


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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    SuperPaths for MED16 About   (see all 6)  
    See pathways by source

    SuperPathContained pathways About
    1Fatty acid, triacylglycerol, and ketone body metabolism
    Fatty acid, triacylglycerol, and ketone body metabolism0.65
    PPARA Activates Gene Expression0.63
    Regulation of Lipid Metabolism by Peroxisome proliferator-activated receptor alpha (PPARalpha)0.65
    Transcriptional Regulation of White Adipocyte Differentiation0.38
    2Gene Expression
    Generic Transcription Pathway0.40
    Gene Expression0.40
    3Metabolism
    Metabolism0.38
    Metabolism of lipids and lipoproteins0.37
    4L1CAM interactions
    Developmental Biology0.63
    5Transcription Ligand Dependent Transcription of Retinoid Target genes
    Transcription Ligand Dependent Transcription of Retinoid Target genes


    Find genes that share SuperPaths with MED16           About GenesLikeMe

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways

    1 Downloadable PowerPoint Slide of GeneGlobe Pathway Central Maps for MED16
        Glucocorticoid Receptor Signaling

    1 GeneGo (Thomson Reuters) Pathway for MED16
        Transcription Ligand-Dependent Transcription of Retinoid-Target genes

    3 Reactome Pathways for MED16
        Transcriptional regulation of white adipocyte differentiation
    Generic Transcription Pathway
    PPARA activates gene expression


    1 Kegg Pathway  (Kegg details for MED16):
        Thyroid hormone signaling pathway

        Pathway & Disease-focused RT2 Profiler PCR Array including MED16: 
              Nuclear Receptors & Coregulators in human mouse rat

    Interactions:

        Search GeneGlobe Interaction Network for MED16

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    Selected Interacting proteins for MED16 (Q9Y2X02, 3 ENSP000003256124) via UniProtKB, MINT, STRING, and/or I2D (see all 665)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    HIST1H3AP684313, ENSP000003502754I2D: score=1 STRING: ENSP00000350275
    HIST1H3BP684313I2D: score=1 
    HIST1H3CP684313I2D: score=1 
    HIST1H3DP684313I2D: score=1 
    HIST1H3EP684313I2D: score=1 
    About this table

    Gene Ontology (GO): Selected biological process terms (see all 8):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006357regulation of transcription from RNA polymerase II promoter NAS10198638
    GO:0006366transcription from RNA polymerase II promoter TAS10198638
    GO:0006367transcription initiation from RNA polymerase II promoter TAS--
    GO:0010467gene expression TAS--
    GO:0030518intracellular steroid hormone receptor signaling pathway IDA11867769

    Find genes that share ontologies with MED16           About GenesLikeMe



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Browse Tocris compounds for MED16



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, and/or Addgene, Primers from OriGene, and/or QIAGEN, Flow cytometry from eBioscience )
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    REFSEQ mRNAs for MED16 gene: 
    NM_005481.2  

    Unigene Cluster for MED16:

    Mediator complex subunit 16
    Hs.365207  [show with all ESTs]
    Unigene Representative Sequence: AF106934
    12 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000395808(uc002lqe.3) ENST00000607471 ENST00000589119 ENST00000606248
    ENST00000312090(uc002lqf.3) ENST00000606828 ENST00000592943 ENST00000586017
    ENST00000592373 ENST00000586342 ENST00000325464(uc002lqd.1) ENST00000269814(uc010drw.2 uc010xfv.1 uc010xfw.1 uc010xfx.1 uc010xfy.1 uc010xga.1)

    miRNA
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    SwitchGear 3'UTR luciferase reporter plasmidMED16 3' UTR sequence
    Inhib. RNA
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    OriGene RNAi products in human, mouse, rat for MED16
    Predesigned siRNA for gene silencing in human, mouse, rat MED16
    Clone
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    OriGene clones in human, mouse for MED16 (see all 7)
    OriGene ORF clones in mouse, rat for MED16
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector: MED16 (NM_005481)
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for MED16
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat MED16
    Primer
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    OriGene qPCR primer pairs and template standards for MED16
    OriGene qSTAR qPCR primer pairs in human, mouse for MED16
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat MED16
      QuantiTect SYBR Green Assays in human, mouse, rat MED16
      QuantiFast Probe-based Assays in human, mouse, rat MED16

    Additional mRNA sequence: 

    AF106934.1 AF121228.1 AL137732.1 BC004554.1 BC007853.2 BC011841.1 BC017282.2 EU392448.1 
    EU392449.1 EU392450.1 EU392451.1 EU392452.1 EU392453.1 EU392454.1 EU392455.1 EU392456.1 
    EU392457.1 EU392458.1 EU392459.1 EU392460.1 EU392461.1 EU392462.1 EU392463.1 EU392464.1 
    EU392465.1 EU392466.1 EU392467.1 EU392468.1 EU392469.1 EU392470.1 EU392471.1 EU392472.1 
    EU392473.1 EU392474.1 EU392475.1 EU392476.1 EU392477.1 EU392478.1 EU392479.1 EU392480.1 
    EU392481.1 EU392482.1 EU392483.1 EU392484.1 EU392485.1 EU392486.1 EU392487.1 EU392488.1 
    EU392489.1 EU392490.1 EU392491.1 EU392492.1 EU392493.1 EU392494.1 EU392495.1 EU392496.1 
    EU392497.1 EU392498.1 

    16 DOTS entries:

    DT.99958172  DT.95222596  DT.97783369  DT.100790175  DT.444361  DT.100032907  DT.100790173  DT.100790174 
    DT.95176134  DT.121485371  DT.92433460  DT.92433462  DT.92433464  DT.95176147  DT.121485389  DT.99981147 

    Selected AceView cDNA sequences (see all 273):

    BU501822 AI346872 AI475781 CD242705 AW071775 BU615705 BU931076 BU552910 
    BU949769 AI356578 CA848542 BM714827 AI590094 AI362133 CA426577 BC017282 
    BQ632153 AI093072 AI568577 BQ277669 CD672782 BM475887 AW028726 AI971791 

    GeneLoc Exon Structure

    Selected Alternative Splicing Database (ASD) splice patterns (SP) for MED16 (see all 8)    About this scheme

    ExUns: 1a · 1b · 1c · 1d ^ 2 ^ 3a · 3b · 3c ^ 4a · 4b ^ 5 ^ 6 ^ 7 ^ 8 ^ 9 ^ 10 ^ 11 ^ 12a · 12b ^ 13 ^ 14 ^ 15a · 15b ^ 16 ^ 17 ^ 18 ^
    SP1:                          -                       -                                                                       -                                 
    SP2:                          -                       -                                                                                         -               
    SP3:                          -                       -                                                                       -                 -               
    SP4:                                                  -                                                                       -                 -               
    SP5:                                                                                                                          -     -     -     -               

    ExUns: 19 ^ 20a · 20b
    SP1:                  
    SP2:                  
    SP3:                  
    SP4:                  
    SP5:                  


    ECgene alternative splicing isoforms for MED16

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
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    MED16 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: --
    MED16 Expression
    About this image

    MED16 Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    MED16 Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.365207
        Pathway & Disease-focused RT2 Profiler PCR Array including MED16: 
              Nuclear Receptors & Coregulators in human mouse rat

    Primer
    Products:
    OriGene qPCR primer pairs and template standards for MED16
    OriGene qSTAR qPCR primer pairs in human, mouse for MED16
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat MED16
    QuantiTect SYBR Green Assays in human, mouse, rat MED16
    QuantiFast Probe-based Assays in human, mouse, rat MED16
    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for MED16

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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    This gene was present in the common ancestor of animals.

    Orthologs for MED16 gene from Selected species (see all 15)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Med161 , 5 mediator complex subunit 161, 5 85.65(n)1
    92.8(a)1
      10 (39.72 cM)5
    2161541  NM_198107.21  NP_932775.21 
     798947085 
    chicken
    (Gallus gallus)
    Aves MED161 mediator complex subunit 16 78.23(n)
    84.8(a)
      420099  XM_418220.4  XP_418220.2 
    lizard
    (Anolis carolinensis)
    Reptilia MED166
    mediator complex subunit 16
    88(a)
    1 ↔ 1
    AAWZ02041879(1089-3534)
    African clawed frog
    (Xenopus laevis)
    Amphibia Xl.163672 Xenopus laevis transcribed sequence with strong similarity more 76.13(n)    BJ033809.1 
    zebrafish
    (Danio rerio)
    Actinopterygii wufc29h122 Transcribed sequence with weak similarity to protein more 76.9(n)    57048457 
    fruit fly
    (Drosophila melanogaster)
    Insecta MED161 Mediator complex subunit 16 45.75(n)
    33.38(a)
      37535  NM_137806.3  NP_611650.2 


    ENSEMBL Gene Tree for MED16 (if available)
    TreeFam Gene Tree for MED16 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section

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    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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    Selected SNPs for MED16 (see all 1387)    About this table                                 

    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 19 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1417891601,2
    --867486(+) GGAAGA/GCCTGC 1 -- ds50010--------
    rs1501563221,2
    C--867505(+) GAAGGC/TGGGAC 1 -- ds50010--------
    rs1871324751,2
    --867532(+) AGAAAC/TGGCAG 1 -- ds50010--------
    rs81057441,2
    C,F--867595(+) TAACAA/C/G/
            
    GGACA
    1 -- ds50019NA WA CSA EA 370
    rs1120615741,2
    C,F--867661(+) AAGTGC/TAGAGA 1 -- ds50013Minor allele frequency- T:0.10CSA WA 122
    rs1895199871,2
    --867685(+) CAGAAA/GCAAGG 1 -- ds50010--------
    rs1806800891,2
    --867697(+) GAGCAC/TGCAGA 1 -- ds50010--------
    rs1383920001,2
    --867698(+) AGCACA/GCAGAG 1 -- ds50010--------
    rs1492378321,2
    --867703(+) GCAGAA/GACAGT 1 -- ds50010--------
    rs16835931,2
    C,F,H--867735(-) TGCCTT/CTCAGT 1 -- ds500122Minor allele frequency- C:0.28MN NS EA NA WA CSA 2412

    HapMap Linkage Disequilibrium report for MED16 (867962 - 893218 bp)

    Structural Variations
         Database of Genomic Variants (DGV) Selected variations for MED16 (see all 35):    About this table    
    Variant IDTypeSubtypePubMed ID
    esv2717804CNV Deletion23290073
    esv2717808CNV Deletion23290073
    esv2670297CNV Deletion23128226
    esv2717801CNV Deletion23290073
    esv2717805CNV Deletion23290073
    esv2717802CNV Deletion23290073
    esv3473CNV Deletion18987735
    esv2717810CNV Deletion23290073
    esv2273959CNV Deletion18987734
    esv2717803CNV Deletion23290073

    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing MED16
    DNA2.0 Custom Variant and Variant Library Synthesis for MED16

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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    OMIM gene information: 604062    OMIM disorders: --


    Find genes that share disorders with MED16           About GenesLikeMe


    Export disorders for MED16 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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    PubMed articles for MED16 gene, integrated from 10 sources (see all 38):
    (articles sorted by number of sources associating them with MED16)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Identity between TRAP and SMCC complexes indicates novel pathways for the function of nuclear receptors and diverse mammalian activators. (PubMed id 10198638)1, 2, 3 Ito M.... Roeder R.G. (Mol. Cell 1999)
    2. Ligand-dependent transcription activation by nuclear receptors requires the DRIP complex. (PubMed id 10235266)1, 2, 3 Rachez C.... Freedman L.P. (Nature 1999)
    3. MED1/TRAP220 exists predominantly in a TRAP/Mediator subpopulation enriched in RNA polymerase II and is required for ER-mediated transcription. (PubMed id 15989967)1, 2 Zhang X....Roeder R.G. (Mol. Cell 2005)
    4. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S.... Malek J. (Genome Res. 2004)
    5. A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology. (PubMed id 15175163)1, 2 Sato S.... Conaway R.C. (Mol. Cell 2004)
    6. Identification of the hypoxia-inducible factor 2I+ nuclear interactome in melanoma cells reveals master proteins involved in melanoma development. (PubMed id 23275444)1 Steunou A.L....Nieto L. (amp 2013)
    7. The protein interaction landscape of the human CMGC kinase group. (PubMed id 23602568)1 Varjosalo M....Gstaiger M. (Cell Rep 2013)
    8. The SCF-Fbw7 ubiquitin ligase degrades MED13 and MED13L and regulates CDK8 module association with Mediator. (PubMed id 23322298)1 Davis M.A....Clurman B.E. (amp 2013)
    9. The HECT type ubiquitin ligase NEDL2 is degraded by anaphase-promoting complex/cyclosome (APC/C)-Cdh1, and its tight regulation maintains the metaphase to anaphase transition. (PubMed id 24163370)1 Lu L....Zhang L. (J. Biol. Chem. 2013)
    10. A census of human soluble protein complexes. (PubMed id 22939629)1 Havugimana P.C....Emili A. (Cell 2012)

    (in PubMed, OMIM, and NCBI Bookshelf)
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     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Entrez Gene: 10025 HGNC: 17556 AceView: THRAP5 Ensembl:ENSG00000175221 euGenes: HUgn10025
    ECgene: MED16 Kegg: 10025 H-InvDB: MED16

    (According to HUGE)
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    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
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    NameDescription
    PharmGKB entry for MED16 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
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    Patent Information for MED16 gene:
    Search GeneIP for patents involving MED16

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, eBioscience, and/or others, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Addgene, Cell lines from GenScript, and ESI BIO, Flow cytometery from eBioscience, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
    Advanced Cell Diagnostics, Animal models from inGenious Targeting Laboratory, genOway)
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