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MED15 Gene

protein-coding   GIFtS: 60
GCID: GC22P020850

Mediator Complex Subunit 15

(Previous names: trinucleotide repeat containing 7, PC2 (positive cofactor...)
(Previous symbols: TNRC7, PCQAP)
  See MED15-related disease
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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Aliases
Mediator Complex Subunit 151 2 3     TIG-12 3
PCQAP1 2 3 5     CTG Repeat Protein 7a2 3
TNRC71 2 3     PC2 Glutamine/Q-Rich-Associated Protein2 3
ARC1052 3 5     TPA-Inducible Gene 1 Protein2 3
TIG12 3 5     CAG7A2
PC2 (Positive Cofactor 2, Multiprotein Complex)
Glutamine/Q-Rich-Associated Protein1 2
     Activator-Recruited Cofactor, 105-KD2
Trinucleotide Repeat Containing 71 2     Mediator Of RNA Polymerase II Transcription Subunit 152
Activator-Recruited Cofactor 105 KDa Component2 3     PC2-Glutamine-Rich-Associated Protein2
Positive Cofactor 2 Glutamine/Q-Rich-Associated Protein2 3     Positive Cofactor 2, Glutamine/Q-Rich-Associated Protein2
Trinucleotide Repeat-Containing Gene 7 Protein2 3     TPA Inducible Gene-12
CTG7A2 3     TPA Inducible Protein2

External Ids:    HGNC: 142481   Entrez Gene: 515862   Ensembl: ENSG000000999177   OMIM: 6073725   UniProtKB: Q96RN53   

Export aliases for MED15 gene to outside databases

Previous GC identifers: GC22P019191 GC22P004129


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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Entrez Gene summary for MED15 Gene:
The protein encoded by this gene is a subunit of the multiprotein complexes PC2 and ARC/DRIP and may function as a
transcriptional coactivator in RNA polymerase II transcription. This gene contains stretches of trinucleotide
repeats and is located in the chromosome 22 region which is deleted in DiGeorge syndrome. Two transcript variants
encoding different isoforms have been found for this gene. (provided by RefSeq, Jul 2008)

GeneCards Summary for MED15 Gene:
MED15 (mediator complex subunit 15) is a protein-coding gene. Diseases associated with MED15 include epicondylitis. GO annotations related to this gene include RNA polymerase II transcription cofactor activity.

UniProtKB/Swiss-Prot: MED15_HUMAN, Q96RN5
Function: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all
RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific
regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by
direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional
preinitiation complex with RNA polymerase II and the general transcription factors. Required for
cholesterol-dependent gene regulation. Positively regulates the Nodal signaling pathway

Gene Wiki entry for MED15 Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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RefSeq DNA sequence at NCBI GenBank:
NC_000022.10  NT_011520.13  NC_018933.2  
Regulatory elements:
   Regulatory transcription factor binding sites in the MED15 gene promoter:
         SRF   SRF (504 AA)   Lmo2   Ik-3   POU2F1   NRF-2   FOXO4   POU2F1a   STAT3   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidMED15 promoter sequence
   Search Chromatin IP Primers for MED15

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat MED15


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 22q11.2   Ensembl cytogenetic band:  22q11.21   HGNC cytogenetic band: 22q11.2

MED15 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
MED15 gene location

GeneLoc information about chromosome 22         GeneLoc Exon Structure

GeneLoc location for GC22P020850:  view genomic region     (about GC identifiers)

Start:
20,850,200 bp from pter      End:
20,941,919 bp from pter
Size:
91,720 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, Cloud-Clone Corp., and/or eBioscience,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, Cloud-Clone Corp., and/or eBioscience, Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, Cloud-Clone Corp, and/or others.)
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UniProtKB/Swiss-Prot: MED15_HUMAN, Q96RN5 (See protein sequence)
Recommended Name: Mediator of RNA polymerase II transcription subunit 15  
Size: 788 amino acids; 86753 Da
Subunit: Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11,
MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26,
MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct
module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this
module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or
more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Interacts with SMAD2,
SMAD3, SREBF1 and SREBF2. Interacts with WWTR1. Interacts with TRIM11
Sequence caution: Sequence=AAC12944.1; Type=Frameshift; Positions=13, 600, 749; Sequence=BAB85034.1;
Type=Miscellaneous discrepancy; Note=Several sequencing errors; Sequence=BAC03446.1; Type=Erroneous initiation;
1 PDB 3D structure from and Proteopedia for MED15:
2GUT (3D)    
Secondary accessions: D3DX31 D3DX32 O15413 Q6IC31 Q8NF16 Q96CT0 Q96IH7 Q9P1T3
Alternative splicing: 3 isoforms:  Q96RN5-1   Q96RN5-2   Q96RN5-3   (No experimental confirmation available)

Explore the universe of human proteins at neXtProt for MED15: NX_Q96RN5

Explore proteomics data for MED15 at MOPED

Post-translational modifications: 

  • Ubiquitinated by TRIM11, leading to proteasomal degradation1
  • Ubiquitination2 at Lys578
  • Modification sites at PhosphoSitePlus

  • See MED15 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins (2 alternative transcripts): 
    NP_001003891.1  NP_056973.2  

    ENSEMBL proteins: 
     ENSP00000407742   ENSP00000409578   ENSP00000392738   ENSP00000391108   ENSP00000408794  
     ENSP00000292733   ENSP00000263205   ENSP00000384344   ENSP00000372434   ENSP00000413636  
     ENSP00000410173   ENSP00000390498   ENSP00000408742   ENSP00000415778   ENSP00000413603  
     ENSP00000396461   ENSP00000408875   ENSP00000416109   ENSP00000443137   ENSP00000416425  
     ENSP00000444604  
    Reactome Protein details: Q96RN5

    MED15 Human Recombinant Protein Products:

    Browse Purified and Recombinant Proteins at EMD Millipore
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    Browse recombinant and purified proteins available from Enzo Life Sciences
    OriGene Purified Protein for MED15
    OriGene Protein Over-expression Lysate for MED15
    OriGene MassSpec for MED15
    OriGene Custom Protein Services for MED15
    GenScript Custom Purified and Recombinant Proteins Services for MED15
    Novus Biologicals MED15 Proteins
    Novus Biologicals MED15 Lysate
    Browse Sino Biological Recombinant Proteins
    Browse Sino Biological Cell Lysates
    Browse ProSpec Recombinant Proteins
    Cloud-Clone Corp. Proteins for MED15

     
    Search eBioscience for Proteins for MED15 

    MED15 Antibody Products:

    Browse EMD Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Browse R&D Systems for Antibodies
    OriGene Antibodies for MED15
    OriGene Custom Antibody Services for MED15
    Novus Biologicals MED15 Antibodies
    Search for Antibodies for MED15 at Abcam
    Cloud-Clone Corp. Antibodies for MED15
    ThermoFisher Antibodies for MED15
    LSBio Antibodies in human, mouse, rat for MED15

    MED15 Assay Products:

    Browse Kits and Assays available from EMD Millipore
    OriGene Custom Assay Services for MED15
    Browse R&D Systems for biochemical assays
    GenScript Custom Assay Services for MED15
    Browse Enzo Life Sciences for kits & assays
    Cloud-Clone Corp. ELISAs for MED15
    Cloud-Clone Corp. CLIAs for MED15
    Search eBioscience for ELISAs for MED15 


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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    1 InterPro protein domain:
     IPR019087 Mediator_Med15_met

    Graphical View of Domain Structure for InterPro Entry Q96RN5

    ProtoNet protein and cluster: Q96RN5

    UniProtKB/Swiss-Prot: MED15_HUMAN, Q96RN5
    Similarity: Belongs to the Mediator complex subunit 15 family


    Find genes that share domains with MED15           About GenesLikeMe


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, Vector BioLabs and/or Addgene, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Flow cytometry from eBioscience, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Molecular Function:

         UniProtKB/Swiss-Prot Summary: MED15_HUMAN, Q96RN5
    Function: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all
    RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific
    regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by
    direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional
    preinitiation complex with RNA polymerase II and the general transcription factors. Required for
    cholesterol-dependent gene regulation. Positively regulates the Nodal signaling pathway
    Induction: By 12-O-tetradecanoylphorbol-13-acetate (TPA)

         Gene Ontology (GO): 2 molecular function terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0001104RNA polymerase II transcription cofactor activity IEA--
    GO:0005515protein binding IPI16799563
         
    Find genes that share ontologies with MED15           About GenesLikeMe


    Phenotypes:
         1 GenomeRNAi human phenotype for MED15:
     Decreased Wnt reporter activit 

    Animal Models:
       inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for MED15
       inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for MED15

       genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for MED15
       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for MED15

    miRNA
    Products:
        
    miRTarBase miRNAs that target MED15:
    hsa-mir-34a-5p (MIRT047371), hsa-mir-324-3p (MIRT042961), hsa-mir-186-5p (MIRT045202), hsa-mir-484 (MIRT042132), hsa-mir-744-5p (MIRT037735)

    Block miRNA regulation of human, mouse, rat MED15 using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate MED15 (see all 20):
    hsa-miR-3685 hsa-miR-125a-5p hsa-miR-301a hsa-miR-298 hsa-miR-125b hsa-miR-130b hsa-miR-3155b hsa-miR-384
    SwitchGear 3'UTR luciferase reporter plasmidMED15 3' UTR sequence
    Inhib. RNA
    Products:
        
    OriGene RNAi products in human, mouse, rat for MED15
    Predesigned siRNA for gene silencing in human, mouse, rat MED15

    Gene Editing
    Products:
    DNA2.0 Custom Protein Engineering Service for MED15

    Clone
    Products:
         
    OriGene clones in human, mouse for MED15 (see all 11)
    OriGene ORF clones in mouse, rat for MED15
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector (see all 2): MED15 (NM_001003891)
    Browse Sino Biological Human cDNA Clones
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for MED15
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat MED15

    Cell Line
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    GenScript Custom overexpressing Cell Line Services for MED15
    Browse ESI BIO Cell Lines and PureStem Progenitors for MED15 
    In Situ Assay
    Products:
       

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for MED15


    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Subcellular locations from UniProtKB/Swiss-Prot
    MED15_HUMAN, Q96RN5: Cytoplasm. Nucleus
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    nucleus5
    golgi apparatus4
    cytosol2

    Gene Ontology (GO): 5 cellular component terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634nucleus IDA--
    GO:0005654nucleoplasm TAS--
    GO:0005737cytoplasm IEA--
    GO:0005794Golgi apparatus ----
    GO:0016592mediator complex IEA--

    Find genes that share ontologies with MED15           About GenesLikeMe


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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    SuperPaths for MED15 About   (see all 8)  
    See pathways by source

    SuperPathContained pathways About
    1Fatty acid, triacylglycerol, and ketone body metabolism
    Fatty acid, triacylglycerol, and ketone body metabolism0.65
    PPARA Activates Gene Expression0.63
    Regulation of Lipid Metabolism by Peroxisome proliferator-activated receptor alpha (PPARalpha)0.65
    Transcriptional Regulation of White Adipocyte Differentiation0.38
    2Gene Expression
    Generic Transcription Pathway0.40
    Gene Expression0.40
    3Metabolism
    Metabolism0.38
    Metabolism of lipids and lipoproteins0.37
    4L1CAM interactions
    Developmental Biology0.63
    5Packaging Of Telomere Ends
    Signal transduction Activin A signaling regulation0.40


    Find genes that share SuperPaths with MED15           About GenesLikeMe

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways


    1 GeneGo (Thomson Reuters) Pathway for MED15
        Signal transduction Activin A signaling regulation

    3 BioSystems Pathways for MED15
        SREBP signalling
    SREBF and miR33 in cholesterol and lipid homeostasis
    Regulation of nuclear SMAD2/3 signaling

    3 Reactome Pathways for MED15
        Transcriptional regulation of white adipocyte differentiation
    Generic Transcription Pathway
    PPARA activates gene expression


        Custom Pathway & Disease-focused RT2 Profiler PCR Arrays for MED15
    Interactions:

        Search GeneGlobe Interaction Network for MED15

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    Selected Interacting proteins for MED15 (Q96RN51, 2, 3 ENSP000002632054) via UniProtKB, MINT, STRING, and/or I2D (see all 138)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    CDK8P493362, 3, ENSP000003709384MINT-6744616 MINT-6744730 MINT-6744749 MINT-6744763 MINT-6754675 I2D: score=1 STRING: ENSP00000370938
    MED1Q156482, 3, ENSP000003006514MINT-6744616 MINT-6744730 MINT-6744749 MINT-6744763 MINT-6754675 I2D: score=1 STRING: ENSP00000300651
    SMAD2Q157962, 3, ENSP000002621604MINT-17344 MINT-17343 I2D: score=3 STRING: ENSP00000262160
    MLLT6P551982, 3, ENSP000003164264MINT-66283 I2D: score=5 STRING: ENSP00000316426
    ATXN1P542532, 3, ENSP000002447694MINT-2873442 I2D: score=3 STRING: ENSP00000244769
    About this table

    Gene Ontology (GO): 4 biological process terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006357regulation of transcription from RNA polymerase II promoter ----
    GO:0006367transcription initiation from RNA polymerase II promoter TAS--
    GO:0010467gene expression TAS--
    GO:0019827stem cell maintenance IEA--

    Find genes that share ontologies with MED15           About GenesLikeMe



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Browse Tocris compounds for MED15

    1 Novoseek inferred chemical compound relationship for MED15 gene    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    glutamine 47 2 11414760 (1), 12497610 (1)



    Find genes that share compounds with MED15           About GenesLikeMe



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, and/or Addgene, Primers from OriGene, and/or QIAGEN, Flow cytometry from eBioscience )
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    REFSEQ mRNAs for MED15 gene (2 alternative transcripts): 
    NM_001003891.1  NM_015889.3  

    Unigene Cluster for MED15:

    Mediator complex subunit 15
    Hs.517421  [show with all ESTs]
    Unigene Representative Sequence: NM_001003891
    Selected Ensembl transcripts including schematic representations, and UCSC links where relevant (see all 34):
    ENST00000445987 ENST00000444094 ENST00000486656 ENST00000414658 ENST00000433831(uc002zsn.1)
    ENST00000432052 ENST00000292733(uc002zsq.3 uc010gso.3 uc002zsr.3 uc011ahs.2)
    ENST00000263205(uc002zsp.3 uc011ahu.2) ENST00000477824 ENST00000406969
    ENST00000382974 ENST00000441501 ENST00000438962 ENST00000445189 ENST00000451058
    ENST00000473028 ENST00000457322 ENST00000428629
    miRNA
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    Block miRNA regulation of human, mouse, rat MED15 using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate MED15 (see all 20):
    hsa-miR-3685 hsa-miR-125a-5p hsa-miR-301a hsa-miR-298 hsa-miR-125b hsa-miR-130b hsa-miR-3155b hsa-miR-384
    SwitchGear 3'UTR luciferase reporter plasmidMED15 3' UTR sequence
    Inhib. RNA
    Products:
         
    OriGene RNAi products in human, mouse, rat for MED15
    Predesigned siRNA for gene silencing in human, mouse, rat MED15
    Clone
    Products:
         
    OriGene clones in human, mouse for MED15 (see all 11)
    OriGene ORF clones in mouse, rat for MED15
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector (see all 2): MED15 (NM_001003891)
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for MED15
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat MED15
    Primer
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    OriGene qPCR primer pairs and template standards for MED15
    OriGene qSTAR qPCR primer pairs in human, mouse for MED15
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat MED15
      QuantiTect SYBR Green Assays in human, mouse, rat MED15
      QuantiFast Probe-based Assays in human, mouse, rat MED15

    Additional mRNA sequence: 

    AF056191.1 AF328769.1 AK074230.1 AK074268.1 AK090465.1 AK294540.1 AK294734.1 AK297284.1 
    AK297789.1 AK315540.1 AL832923.1 BC003078.1 BC007529.1 BC013985.2 BC017110.1 CR456537.1 
    U80745.1 

    Selected DOTS entries (see all 49):

    DT.100686525  DT.95158731  DT.120668347  DT.100774255  DT.100793193  DT.120667758  DT.87000953  DT.100793189 
    DT.100836246  DT.100766669  DT.102843155  DT.91932860  DT.120667791  DT.120668270  DT.75171660  DT.95255972 
    DT.92443094  DT.100793187  DT.101981829  DT.101981830  DT.101985526  DT.120668557  DT.120668198  DT.120667802 

    Selected AceView cDNA sequences (see all 437):

    BM764227 BQ689421 BM742235 BM711393 BE207309 CF995849 CD370372 BQ065211 
    AI584002 AI590045 BQ686315 BQ016521 BC007529 AL134960 BI830996 CF126048 
    BQ688842 BU174657 BQ691267 AA497031 BQ690279 BE019568 BU185760 BM848637 

    GeneLoc Exon Structure

    Selected Alternative Splicing Database (ASD) splice patterns (SP) for MED15 (see all 20)    About this scheme

    ExUns: 1a · 1b · 1c · 1d · 1e ^ 2 ^ 3 ^ 4 ^ 5a · 5b ^ 6a · 6b · 6c ^ 7a · 7b · 7c ^ 8 ^ 9 ^ 10 ^ 11a · 11b ^ 12 ^ 13a · 13b ^ 14a · 14b ^
    SP1:                                                                                                                                                            
    SP2:                                -           -           -     -     -     -           -     -     -                                                         
    SP3:                                                        -     -     -     -     -     -     -     -     -                                                   
    SP4:                                -     -     -           -     -     -     -           -     -     -                                                         
    SP5:                                -     -     -           -     -     -     -     -     -     -     -     -                                                   

    ExUns: 15 ^ 16a · 16b ^ 17a · 17b ^ 18a · 18b · 18c ^ 19 ^ 20 ^ 21a · 21b ^ 22 ^ 23
    SP1:                                            -                       -               
    SP2:                                                                                    
    SP3:                                                                                    
    SP4:                                                                                    
    SP5:                                                                                    


    ECgene alternative splicing isoforms for MED15

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

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    MED15 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: CAGGGAGCGC
    MED15 Expression
    About this image


    MED15 expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database
     selected tissues (see all 7) fully expand
     
     Brain (Nervous System)    fully expand to see all 4 entries
             Cerebral Cortex
     
     Ovary (Reproductive System)    fully expand to see all 2 entries
             Ovarian Mesenchymal Stroma Cells Ovary Interstitium
             Oviduct
     
     Trophoblast (Extraembryonic Tissues)
             Trophoblast Cells Trophoblast
     
     Pancreas (Endocrine System)
             Islets of Langerhans
     
     Testis (Reproductive System)
             Leydig Cells Testis Interstitium
    MED15 Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    MED15 Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.517421

    UniProtKB/Swiss-Prot: MED15_HUMAN, Q96RN5
    Tissue specificity: Expressed in all tissues examined, including heart, brain, lung, spleen, thymus, pancreas,
    blood leukocyte and placenta. However, the level of expression varied, with highest expression in the placenta
    and peripheral blood and lowest in the pancreas and kidney

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    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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    This gene was present in the common ancestor of animals.

    Orthologs for MED15 gene from Selected species (see all 13)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Med155 mediator complex subunit 15   --   16 (10.94 cM) 17651221 
    chicken
    (Gallus gallus)
    Aves MED156
    mediator complex subunit 15
    65(a)
    1 ↔ 1
    15(8297696-8323443)
    African clawed frog
    (Xenopus laevis)
    Amphibia ARC1052 ARC105 protein 74.85(n)    AF378334.1 
    zebrafish
    (Danio rerio)
    Actinopterygii Dr.74972 Danio rerio mRNA similar to PC2 (positive cofactor more 76.75(n)    BC045472.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta MED156
    Mediator complex subunit 15
    31(a)
    1 ↔ 1
    2L(473013-476170)


    ENSEMBL Gene Tree for MED15 (if available)
    TreeFam Gene Tree for MED15 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
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    Paralogs for MED15 gene
    Selected SIMAP similar genes for MED15 using alignment to 19 protein entries:     MED15_HUMAN (see all proteins) (see all similar genes):
    DKFZp762B1216    DKFZp686A2214    FAM129C    UTY    ZNF738    ZNF74
    NEK4    SHANK2    ATP6V0A1    FAM175A    HECW1    KLHL2
    NPLOC4    TNFRSF17    ZNF20    APOD    CATSPERG    CFLAR

    Find genes that share paralogs with MED15           About GenesLikeMe


    Selected Pseudogenes.org Pseudogenes for MED15 (see all 8)
    PGOHUM00000250102 PGOHUM00000248026 PGOHUM00000250332 PGOHUM00000250446 PGOHUM00000240325


    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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    Polymorphic Variants from UniProtKB/Swiss-Prot
    MED15_HUMAN, Q96RN5: The poly-Gln region from amino acids 235-262 of PCQAP is polymorphic. There are from 15 to 18
    repeats in the Italian population


    Selected SNPs for MED15 (see all 1917)    About this table                                 

    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 22 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1910673171,2
    --20859903(+) CTCACG/TCCTGT 2 -- us2k10--------
    rs790294471,2
    C--20859981(+) AACACA/GGTGAA 2 -- us2k10--------
    rs795381791,2
    C--20859989(+) GAAACA/CCTGTC 2 -- us2k10--------
    rs2015900511,2
    --20860364(+) ACCTG-/TTAATC 2 -- us2k10--------
    rs18075111,2
    C,F,H--20860379(+) ctactC/Tgggag 2 -- us2k17Minor allele frequency- T:0.15NA WA CSA 13
    rs1445885181,2
    --20860473(+) CAGGGC/TGAGAC 2 -- us2k10--------
    rs1120979361,2
    C--20860487(+) ATCTC-/AAAAAA 2 -- us2k11Minor allele frequency- A:0.50CSA 2
    rs1831870021,2
    --20860661(+) TGGGGC/TACGCT 2 -- us2k10--------
    rs1884935301,2
    --20860742(+) GCCGAC/GATGGC 2 -- us2k10--------
    rs1930540041,2
    --20860746(+) AGATGG/TCACCA 2 -- us2k10--------

    HapMap Linkage Disequilibrium report for MED15 (20850200 - 20941919 bp)

    Structural Variations
         Database of Genomic Variants (DGV) Selected variations for MED15 (see all 20):    About this table    
    Variant IDTypeSubtypePubMed ID
    nsv821342CNV Deletion20802225
    nsv509810CNV Insertion20534489
    nsv3561CNV Insertion18451855
    nsv436864CNV Insertion17901297
    nsv436867CNV Insertion17901297
    nsv834139CNV Loss17160897
    nsv834138CNV Loss17160897
    nsv914402CNV Loss21882294
    nsv3562CNV Loss18451855
    nsv523162CNV Loss19592680

    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing MED15
    DNA2.0 Custom Variant and Variant Library Synthesis for MED15

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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    OMIM gene information: 607372    OMIM disorders: --

    1 disease for MED15:    
    About MalaCards
    epicondylitis

    2 diseases from the University of Copenhagen DISEASES database for MED15:
    Epicondylitis     Velocardiofacial syndrome

    Find genes that share disorders with MED15           About GenesLikeMe

    Genetic Association Database (GAD): MED15
    Human Genome Epidemiology (HuGE) Navigator: MED15 (3 documents)

    Export disorders for MED15 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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    PubMed articles for MED15 gene, integrated from 10 sources (see all 48):
    (articles sorted by number of sources associating them with MED15)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Isolation and characterization of a novel gene from the DiGeorge chromosomal region that encodes for a mediator subunit. (PubMed id 11414760)1, 2, 3, 9 Berti L....Meisterernst M. (Genomics 2001)
    2. A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology. (PubMed id 15175163)1, 2, 3 Sato S.... Conaway R.C. (Mol. Cell 2004)
    3. A novel glutamine-rich putative transcriptional adaptor protein (TIG- 1), preferentially expressed in placental and bone-marrow tissues. (PubMed id 11024300)1, 2, 3 Abraham S. and Solomon W.B. (Gene 2000)
    4. An ARC/Mediator subunit required for SREBP control of cholesterol and lipid homeostasis. (PubMed id 16799563)1, 2, 9 Yang F....Naeaer A.M. (Nature 2006)
    5. Association study between CAG trinucleotide repeats in the PCQAP gene (PC2 glutamine/Q-rich-associated protein) and schizophrenia. (PubMed id 12497610)1, 4, 9 De Luca A....Novelli G. (Am. J. Med. Genet. B Neuropsychiatr. Genet. 2003)
    6. TAZ controls Smad nucleocytoplasmic shuttling and regulates human embryonic stem-cell self-renewal. (PubMed id 18568018)1, 2 Varelas X.... Wrana J.L. (Nat. Cell Biol. 2008)
    7. TRIM11 binds to and destabilizes a key component of the activator- mediated cofactor complex (ARC105) through the ubiquitin-proteasome system. (PubMed id 16904669)1, 2 Ishikawa H.... Takahashi N. (FEBS Lett. 2006)
    8. MED1/TRAP220 exists predominantly in a TRAP/Mediator subpopulation enriched in RNA polymerase II and is required for ER-mediated transcription. (PubMed id 15989967)1, 2 Zhang X....Roeder R.G. (Mol. Cell 2005)
    9. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (Nat. Genet. 2004)
    10. An association study of PCQAP polymorphisms and schizophrenia. (PubMed id 15318033)1, 4 Sandhu H.K....Philibert R.A. (Psychiatr. Genet. 2004)

    (in PubMed, OMIM, and NCBI Bookshelf)
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     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Entrez Gene: 51586 HGNC: 14248 AceView: PCQAP Ensembl:ENSG00000099917 euGenes: HUgn51586
    ECgene: MED15 H-InvDB: MED15

    (According to HUGE)
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      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
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    NameDescription
    PharmGKB entry for MED15 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section

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    Patent Information for MED15 gene:
    Search GeneIP for patents involving MED15

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, eBioscience, and/or others, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Addgene, Cell lines from GenScript, and ESI BIO, Flow cytometery from eBioscience, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
    Advanced Cell Diagnostics, Animal models from inGenious Targeting Laboratory, genOway)
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