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Category Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21

GIFtS
-

MED14 Gene

protein-coding GIFtS: 57
GCID: GC0XM040507

mediator complex subunit 14

(Previous names: cofactor required for Sp1 transcriptional activation, subunit...)
(Previous symbols: CXorf4, CRSP2)

Explore 5 diseases affiliated with
MED14 via

MalaCards

our new
Human Malady Compendium

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc, ⁷Ensembl, ⁸DME, ⁹miRBase, and/or ¹⁰fRNAdb)
About This Section

Aliases
Mediator Complex Subunit 14¹ ² ³		CRSP Complex Subunit 2² ³
CRSP2¹ ² ³ ⁵		HRGR1¹
CXorf4¹ ² ³ ⁵		RGR1 Homolog² ³
EXLM1¹ ² ³ ⁵		Cofactor Required For Sp1 Transcriptional Activation, Subunit 2, 150kDa¹
TRAP170¹ ² ³ ⁵		Cofactor Required For Sp1 Transcriptional Activation, Subunit 2 (150kD)²
RGR1¹ ² ³		Human Homolog Of Yeast RGR1²
CRSP150¹ ²		Mediator Of RNA Polymerase II Transcription Subunit 14²
CSRP¹ ²		Thyroid Hormone Receptor-Associated Protein Complex Component TRAP170²
Activator-Recruited Cofactor 150 KDa Component² ³		Transcriptional Co-Activator CRSP150²
Thyroid Hormone Receptor-Associated Protein Complex 170 KDa Component² ³		Vitamin D Receptor-Interacting Protein Complex Component DRIP150²
Vitamin D3 Receptor-Interacting Protein Complex 150 KDa Component² ³		Trap170³
ARC150² ³		Cofactor Required For Sp1 Transcriptional Activation Subunit 2³
DRIP150² ³		Transcriptional Coactivator CRSP150³

External Ids:	HGNC: 2370¹	Entrez Gene: 9282²	Ensembl: ENSG00000180182⁷	OMIM: 300182⁵	UniProtKB: O60244³

Export aliases for MED14 gene to outside databases

Previous GC identifers: GC0XM040393 GC0XM038238

(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for MED14:

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional

enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA

polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1

activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a

component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with

TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. This protein

contains a bipartite nuclear localization signal. This gene is known to escape chromosome X-inactivation. (provided by

RefSeq, Jul 2008)

UniProtKB/Swiss-Prot: MED14_HUMAN, O60244

Function: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA

polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory

proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct

interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex

with RNA polymerase II and the general transcription factors

Gene Wiki entry for MED14

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section

RefSeq DNA sequence:

NC_000023.10 NC_018934.1 NT_079573.4

Regulatory elements:

SABiosciences Regulatory transcription factor binding sites in the MED14 gene promoter:
ISGF-3 Tal-1 MyoD FOXD3 Tal-1beta LCR-F1 E47 HOXA5 ITF-2
Other transcription factors

SwitchGear Promoter luciferase reporter plasmid: MED14 promoter sequence

Search SABiosciences Chromatin IP Primers for MED14

Epigenetics:

QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat MED14

Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: Xp11.4 Ensembl cytogenetic band: Xp11.4 HGNC cytogenetic band: Xp11.4

MED14 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
MED14 gene location

GeneLoc information about chromosome X GeneLoc Exon Structure

GeneLoc location for GC0XM040507: view genomic region (about GC identifiers)

Start:	40,507,558 bp from pter	End:	40,595,110 bp from pter
Size:	87,553 bases	Orientation:	minus strand

(According to ¹UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to ²PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: MED14_HUMAN, O60244 (See protein sequence)

Recommended Name: Mediator of RNA polymerase II transcription subunit 14

Size: 1454 amino acids; 160607 Da

Subunit: Interacts with GATA1 (By similarity). Component of the Mediator complex, which is composed of MED1, MED4,

MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21,

MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and

CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than

Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex

lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Interacts with

AR, ESR1, SREBF1 and STAT2

Subcellular location: Nucleus (Probable)

Secondary accessions: Q4KMR7 Q9UNB3

Explore the universe of human proteins at neXtProt for MED14: NX_O60244

Post-translational modifications:

View modification sites using PhosphoSitePlus²

View neXtProt modification sites for NX_O60244

MED14 Protein expression data from MOPED and PaxDb: About this image

REFSEQ proteins: NP_004220.2

ENSEMBL proteins:

ENSP00000323720 ENSP00000392586 ENSP00000411357

Reactome Protein details: O60244
Human Recombinant Protein Products for MED14:

	Browse Purified and Recombinant Proteins at EMD Millipore
	Browse R&D Systems for human recombinant proteins
	Browse recombinant and purified proteins available from Enzo Life Sciences
	Browse OriGene full length recombinant human proteins expressed in human HEK293 cells Browse OriGene Protein Over-expression Lysates OriGene Custom Protein Services for MED14
	GenScript Custom Purified and Recombinant Proteins Services for MED14
	Browse Sino Biological Recombinant Proteins
	Browse ProSpec Recombinant Proteins
	Uscn Proteins for MED14

Gene Ontology (GO): 3 cellular component terms (GO ID links to tree view): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005634	nucleus	IDA	10235267
GO:0005654	nucleoplasm	TAS	--
GO:0016592	mediator complex	IDA	10198638

MED14 for ontologies About GeneDecksing

MED14 Antibody Products:

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	GenScript Custom Superior Antibodies Services for MED14
	Novus Biologicals MED14 Antibody
	Abcam antibodies for MED14
	Uscn Antibodies for MED14
	Search ThermoFisher Antibodies for MED14

Assay Products for MED14:

	Browse Kits and Assays available from EMD Millipore
	OriGene Custom Immunoassay Development Browse OriGene Fluorogenic Cell Assay Kits
	Browse R&D Systems for biochemical assays
	GenScript Custom Assay Services for MED14
	Browse Enzo Life Sciences for kits & assays
	Uscn ELISAs and CLIAs for MED14

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

MED14 for domains About GeneDecksing

1 InterPro domain/family:

IPR013947 Mediator_Med14

Graphical View of Domain Structure for InterPro Entry O60244

ProtoNet protein and cluster: O60244

1 Blocks protein family: IPB013947 Mediator complex

UniProtKB/Swiss-Prot: MED14_HUMAN, O60244

Similarity: Belongs to the Mediator complex subunit 14 family

(According to ¹UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or ²DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory,
bound targets from SABiosciences, miRNA Gene Targets from miRTarBase, shRNA from OriGene, Sirion Biotech, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Sirion Biotech, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, Sirion Biotech, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
About This Section

Molecular Function:

UniProtKB/Swiss-Prot Summary: MED14_HUMAN, O60244

Function: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA

polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory

proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct

interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex

with RNA polymerase II and the general transcription factors

Gene Ontology (GO): 5/7 molecular function terms (GO ID links to tree view) (see all 7): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0001104	RNA polymerase II transcription cofactor activity	IDA	10198638
GO:0003712	transcription cofactor activity	IDA	12218053
GO:0003713	transcription coactivator activity	IDA	12037571
GO:0004872	receptor activity	IDA	12218053
GO:0030374	ligand-dependent nuclear receptor transcription coactivator activity	NAS	10235266

MED14 for ontologies About GeneDecksing

Phenotypes:
8 GenomeRNAi human phenotypes for MED14:

Decreased NANOG protein expres	Decreased OCT4 protein express	Decreased POU5F1-GFP protein e	Decreased influenza A/WSN/33 r
Decreased p24 protein expressi	Decreased viability of wild-ty	G0/1 arrest	Increased G1 DNA content, incr

1 MGI mutant phenotype (inferred from 1 allele) (MGI details for Med14):

normal

MED14 for phenotypes About GeneDecksing

Animal Models:

inGenious Targeting Laboratory - Customizable classic, inducible, and humanized mouse model solutions for MED14

miRNA Products:		OriGene 3'-UTR Clone: MED14 Browse MicroRNA Expression Plasmids
		QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat MED14
		8/58 QIAGEN miScript miRNA Assays for microRNAs that regulate MED14 (see all 58):
		hsa-miR-142-5p hsa-miR-193a-3p hsa-miR-4328 hsa-miR-300 hsa-miR-4272 hsa-miR-128 hsa-miR-16-1* hsa-miR-578

		SwitchGear 3'UTR luciferase reporter plasmid: MED14 3' UTR sequence

Inhib. RNA Products:		Browse for Gene Knock-down Tools from EMD Millipore
		OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for MED14 OriGene shRNA RFP: MED14 OriGene siRNA: MED14
		QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat MED14
		Sirion Biotech Custom design and validation of potent shRNA sequences against MED14

Gene Editing Products:			DNA2.0 Custom Protein Engineering Service for MED14
			Sirion Biotech Customized adenovirus for overexpression of MED14 Sirion Biotech Customized adenovirus for potent knockdown of MED14

Clone Products:		Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore
		OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for MED14 (see all 3) OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for MED14 OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
		GenScript: all cDNA clones in your preferred vector: MED14 (NM_004229)
		Browse Sino Biological Human cDNA Clones
		DNA2.0 Custom Codon Optimized Gene Synthesis Service for MED14
		Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat MED14

Cell Line Products:		GenScript Custom overexpressing Cell Line Services for MED14
		Search LifeMap BioReagents cell lines for MED14
		Sirion Biotech Customized stable knockdown cell line services for MED14 Sirion Biotech Customized inducible knockdown cell line services for MED14 Sirion Biotech Customized stable overexpressing cell line services for MED14 Sirion Biotech Customized inducible overexpressing cell line services for MED14

In Situ Assay
Products:

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for MED14

(Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to ¹UniProtKB, ²MINT, ³I2D, and/or ⁴STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
About This Section

Unified GeneCards pathways About this table

See pathways by source

Super-pathway

contained gene-specific pathways

Generic Transcription Pathway

Generic Transcription Pathway

1.00

Gene Expression

0.46

HIV Infection

Transcription Ligand-Dependent Transcription of Retinoid-Target genes

0.28

Transcription_Ligand-Dependent Transcription of Retinoid-Target genes

0.28

Transcriptional Regulation of White Adipocyte Differentiation

Transcriptional Regulation of White Adipocyte Differentiation

1.00

Axon guidance

Developmental Biology

0.69

Pathway sources
See GeneCards unified pathways
Show all pathways

1 EMD Millipore Pathway for MED14

Transcription Ligand-Dependent Transcription of Retinoid-Target genes

1 GeneGo (Thomson Reuters) Pathway for MED14

Transcription Ligand-Dependent Transcription of Retinoid-Target genes

4 Reactome Pathways for MED14

	Developmental Biology
	Transcriptional Regulation of White Adipocyte Differentiation
	Generic Transcription Pathway
	Gene Expression

MED14 for pathways About GeneDecksing

Interactions:

SABiosciences Gene Network Central^TM Interacting Genes and Proteins Network for MED14

STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

5/115 Interacting proteins for MED14 (O60244^{1, 2, 3} ENSP00000323720⁴) via UniProtKB, MINT, STRING, and/or I2D (see all 115)

Interactant		Interaction Details
GeneCard	External ID(s)	Interaction Details
ABL1	P00519¹^,²^,³, ENSP00000361423⁴	EBI-394489,EBI-375543 MINT-8110167 I2D: score=3 STRING: ENSP00000361423
FYN	P06241¹^,²^,³, ENSP00000357656⁴	EBI-394489,EBI-515315 MINT-8111067 I2D: score=3 STRING: ENSP00000357656
MED1	Q15648²^,³, ENSP00000300651⁴	MINT-6744331 MINT-6744763 I2D: score=2 STRING: ENSP00000300651
CDK8	P49336²^,³, ENSP00000370938⁴	MINT-6744763 I2D: score=1 STRING: ENSP00000370938
MED15	Q96RN5²^,³, ENSP00000263205⁴	MINT-6744763 I2D: score=1 STRING: ENSP00000263205

About this table

Gene Ontology (GO): 5/8 biological process terms (GO ID links to tree view) (see all 8): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0006357	regulation of transcription from RNA polymerase II promoter	TAS	9989412
GO:0006367	transcription initiation from RNA polymerase II promoter	TAS	--
GO:0010467	gene expression	TAS	--
GO:0019827	stem cell maintenance	IEA	--
GO:0030518	intracellular steroid hormone receptor signaling pathway	IDA	11867769

MED14 for ontologies About GeneDecksing

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
About This Section

MED14 for compounds About GeneDecksing

Browse Small Molecules at EMD Millipore

Browse drugs & compounds from Enzo Life Sciences

Browse Tocris compounds for MED14
2 Novoseek chemical compound relationships for MED14 gene About this table

Compound	-log (P-Val)	Hits	PubMed IDs for Articles with Shared Sentences (# sentences)
vitamin d	43.9	3	17306756 (2), 15625066 (1)
estrogen	28.3	6	15625066 (3), 17306756 (2)

Search CenterWatch for drugs/clinical trials and news about MED14

(Secondary structures according to fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from EMD Millipore,
siRNAs from OriGene, QIAGEN, shRNA from OriGene, Sirion Biotech, microRNA from QIAGEN,
Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
About This Section

REFSEQ mRNAs for MED14 gene:

NM_004229.3

Unigene Cluster for MED14:

Mediator complex subunit 14

Hs.407604 [show with all ESTs]

Unigene Representative Sequence: AB006651

9 Ensembl transcripts including schematic representations, and UCSC links where relevant:

ENST00000324817(uc004dex.4 uc010nhe.1) ENST00000416199 ENST00000433003(uc004dey.1)

ENST00000472736 ENST00000496531 ENST00000482034 ENST00000492219 ENST00000495865

ENST00000463072

miRNA Products:		OriGene 3'-UTR Clone: MED14 Browse OriGene MicroRNA Expression Plasmids
		QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat MED14
		8/58 QIAGEN miScript miRNA Assays for microRNAs that regulate MED14 (see all 58):
		hsa-miR-142-5p hsa-miR-193a-3p hsa-miR-4328 hsa-miR-300 hsa-miR-4272 hsa-miR-128 hsa-miR-16-1* hsa-miR-578

		SwitchGear 3'UTR luciferase reporter plasmid: MED14 3' UTR sequence

Inhib. RNA Products:		Browse for Gene Knock-down Tools from EMD Millipore
		OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for MED14 OriGene shRNA RFP: MED14 OriGene siRNA: MED14
		QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat MED14
		Sirion Biotech Custom design and validation of potent shRNA sequences against MED14

Clone Products:		OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for MED14 (see all 3) OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for MED14 OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
		GenScript: all cDNA clones in your preferred vector: MED14 (NM_004229)
		DNA2.0 Custom Codon Optimized Gene Synthesis Service for MED14
		Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat MED14

Primer Products:		OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for MED14 Browse OriGene validated miRNA SYBR primer pairs
		SABiosciences RT² qPCR Primer Assay in human, mouse, rat MED14
		QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat MED14
		QIAGEN QuantiFast Probe-based Assays in human, mouse, rat MED14

Additional cDNA sequence:

AB006651.1 AF070563.1 AF104256.1 AF135802.1 AF304448.1 AJ012077.1 AK293070.1 AK308871.1

BC098377.1 BC132672.1 BC132674.1 BC144231.1

15 DOTS entries:

DT.416891 DT.207422 DT.101964852 DT.91758292 DT.100773919 DT.99934405 DT.100725407 DT.121289920

DT.121289945 DT.40263154 DT.75114850 DT.91683768 DT.96086380 DT.97813479 DT.86846470

24/265 AceView cDNA sequences (see all 265):

AB006651 AI634602 AA150093 AW613955 AI366067 AF304448 AW963669 AI569341

AF104256 AA737180 BX487392 BM765536 BM742336 AI493808 BM784815 Z41980

AI263090 BE792405 AA857946 AI282836 AI718098 BQ216394 AA460683 BE549785

GeneLoc Exon Structure

(RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section

MED14 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: AAATGTTCTG

About this image

MED14 expression in embryonic tissues and stem cells
Expression by the Database of Embryonic development, Stem cell research, and Regenerative medicine About this table

2 LifeMap In Vivo Development Anatomical Compartments/Cells

Tissue

Anatomical Compartment

Cell

Category (developmental path)

Kidney

Cap Mesenchyme

Cap Mesenchyme Cells

Kidney

Kidney

S-shaped Body

Kidney

Expression:

Positive

Negative

Selective marker

Experimental details:

Curated

Microarrays

In-situ hybridization

See MED14 Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for MED14

SOURCE GeneReport for Unigene cluster: Hs.407604

UniProtKB/Swiss-Prot: MED14_HUMAN, O60244

Tissue specificity: Ubiquitous

SABiosciences Expression via Pathway-Focused PCR Array including MED14:

Nuclear Receptors & Coregulators in human mouse rat

Primer Products:		OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for MED14 Browse OriGene validated miRNA SYBR primer pairs
		SABiosciences RT² qPCR Primer Assay in human, mouse, rat MED14
		QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat MED14
		QIAGEN QuantiFast Probe-based Assays in human, mouse, rat MED14

In Situ
Assay Products:

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for MED14

(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD , ⁵MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or ⁶Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
About This Section

This gene was present in the common ancestor of eukaryotes.

Orthologs for MED14 gene from 9/28 species (see all 28) About this table

Organism	Taxonomic classification	Gene	Description	Human Similarity	Orthology Type	Details
mouse (Mus musculus)	Mammalia	Med14^{1 , 5}	mediator complex subunit 14^{1, 5}	90.87(n)¹ 96.9(a)¹		X (7.52 cM)⁵ 26896¹ NM_001048208.1¹ NP_001041673.1¹ 12675369⁵
chicken (Gallus gallus)	Aves	MED14¹	mediator complex subunit 14	81.92(n) 91.05(a)		418572 XM_416776.2 XP_416776.2
lizard (Anolis carolinensis)	Reptilia	MED14⁶	--	90(a)	1 ↔ 1	3(133853784-133898983)
African clawed frog (Xenopus laevis)	Amphibia	Xl.5858²	Xenopus laevis transcribed sequence with moderate similarity more	79.52(n)		CA971285.1
zebrafish (Danio rerio)	Actinopterygii	Dr.8754²	Danio rerio mRNA similar to cofactor required for Sp1 more	78.26(n)		BC045931.1
fruit fly (Drosophila melanogaster)	Insecta	MED14¹	Mediator complex subunit 14	48.9(n) 42.59(a)		38073 NM_138197.2 NP_612041.2
worm (Caenorhabditis elegans)	Secernentea	rgr-1⁶	Mediator of RNA polymerase II transcription subuni...	19(a)	1 ↔ 1	III(9393395-9404448)
thale cress (Arabidopsis thaliana)	eudicotyledons	SWP⁶	RNA polymerase II transcription mediator	11(a)	1 ↔ 1	3(1293807-1300714)
rice (Oryza sativa)	Liliopsida	-- -- (see all 3)	SWP, putative, expressed (see all 3)	24(a) 13(a) (see all 3)	possible ortholog possible ortholog (see all 3)	8(14735821-14739559) 9(6036880-6048276)

ENSEMBL Gene Tree for MED14 (if available)
TreeFam Gene Tree for MED14 (if available)

(Paralogs according to ¹HomoloGene,
²Ensembl, and ³SIMAP, Pseudogenes according to ⁴Pseudogene.org Build 68)
About This Section

(SNPs/Variants according to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section

SNP ID	Valid	Clinical significance	Chr X pos	Sequence	#	AA Chg	Type	More	#	Allele freq	Pop	Total sample	More
Genomic Data					Transcription Related Data				Allele Frequencies
				--	--	--		--	--
rs7053646^1,2	C,H	--	40508494(+)	`CACAGT/GTATTA`	2	--	ds500¹ us2k¹		6		NS EA NA	420
rs184805157^1,2		--	40509049(+)	`TTCAAA/CTTCAT`	1	--	ut3¹		0	--	--	--	--
rs188097804^1,2		--	40509092(+)	`TTTGCA/GCACTG`	1	--	ut3¹		0	--	--	--	--
rs11549699^1,2	C	--	40509180(-)	`TACACA/TTGATG`	1	--	ut3¹ ese³		0	--	--	--	--
rs181001096^1,2		--	40509198(+)	`AATTAA/GCATTT`	1	--	ut3¹		0	--	--	--	--
rs73624918^1,2	C	--	40509242(+)	`GTTAAT/CTCTTA`	1	--	ut3¹		1		WA	2
rs184875754^1,2		--	40509309(+)	`CATGTA/GGATTT`	1	--	ut3¹		0	--	--	--	--
rs189383684^1,2		--	40509393(+)	`CTGATA/GTGATT`	1	--	ut3¹		0	--	--	--	--
rs181399811^1,2		--	40509491(+)	`GAGTTC/GGGTGA`	1	--	ut3¹		0	--	--	--	--
rs186598906^1,2		--	40509913(+)	`CCATTA/TGACCC`	1	--	ut3¹		0	--	--	--	--

HapMap Linkage Disequilibrium report for MED14 (40507558 - 40595110 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV): 1 variation for MED14
1 CNV: 7785
Locus Specific Mutation Databases (LSDB): MED14

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(in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
About This Section

MED14 for disorders           About GeneDecksing

OMIM gene information: 300182    OMIM disorders: --

5 diseases for MED14:    About MalaCards

thyroiditis x inactivation conjunctivitis breast cancer

cholesterol

1 Novoseek disease relationship for MED14 gene About this table

Disease	-log (P-Val)	Hits	PubMed IDs for Articles with Shared Sentences (# sentences)
breast cancer	17.7	4	15625066 (2), 17306756 (2)

Human Genome Epidemiology (HuGE) Navigator: MED14 (1 document)

Export disorders for MED14 gene to outside databases

(in PubMed. Associations of this gene to articles via ¹Entrez Gene, ²UniProtKB/Swiss-Prot, ³HGNC, ⁴GAD, ⁵PharmGKB, ⁶HMDB, ⁷DrugBank, ⁸UniProtKB/TrEMBL, ⁹ Novoseek, and/or ¹⁰fRNAdb)
About This Section

PubMed articles for MED14 gene, integrated from 9 sources (see all 61):
(articles sorted by number of sources associating them with MED14)

Utopia: connect your pdf to the dynamic
world of online information

The transcriptional cofactor complex CRSP is required for activity of the enhancer-binding protein Sp1. (PubMed id 9989412)^{1, 2, 3} Ryu S.... Tjian R. (1999)
Detection and isolation of a novel human gene located on Xp11.2-p11.4 that escapes X-inactivation using a two-dimensional DNA mapping method. (PubMed id 9598311)^{1, 2, 3} Yoshikawa H....Matsubara K. (1998)
DRIP150 coactivation of estrogen receptor alpha in ZR-75 breast cancer cells is independent of LXXLL motifs. (PubMed id 15625066)^{1, 2, 9} Lee J.E.... Safe S. (2005)
Role of metazoan mediator proteins in interferon-responsive transcription. (PubMed id 12509459)^{1, 2, 9} Lau J.F.... Horvath C.M. (2003)
A coregulatory role for the TRAP-mediator complex in androgen receptor-mediated gene expression. (PubMed id 12218053)^{1, 2, 9} Wang Q.... Fondell J.D. (2002)
MED1/TRAP220 exists predominantly in a TRAP/Mediator subpopulation enriched in RNA polymerase II and is required for ER-mediated transcription. (PubMed id 15989967)^{1, 2} Zhang X....Roeder R.G. (2005)
A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology. (PubMed id 15175163)^{1, 2} Sato S....Conaway R.C. (2004)
The TRAP/Mediator coactivator complex interacts directly with estrogen receptors alpha and beta through the TRAP220 subunit and directly enhances estrogen receptor function in vitro. (PubMed id 11867769)^{1, 2} Kang Y.K.... Roeder R.G. (2002)
A novel human SRB/MED-containing cofactor complex, SMCC, involved in transcription regulation. (PubMed id 10024883)^{1, 2} Gu W.... Roeder R.G. (1999)
Ligand-dependent transcription activation by nuclear receptors requires the DRIP complex. (PubMed id 10235266)^{1, 2} Rachez C.... Freedman L.P. (1999)

(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

		AND	OR
Aliases
Disorders
Free Text

Query String		PubMed OMIM NCBI Bookshelf
	(Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
About This Section

Entrez Gene: 9282	HGNC: 2370	AceView: CRSP2	Ensembl:ENSG00000180182	euGenes: HUgn9282
ECgene: MED14	H-InvDB: MED14

(According to HUGE)
About This Section

(According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

Name	Description
PharmGKB entry for MED14	Pharmacogenomics, SNPs, Pathways

Intellectual Property for MED14 gene
(Patent information from GeneIP, Licensable technologies from WIS Yeda, Salk, Tufts, IP news from LifeMap Sciences, Inc.)

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Patent Information for MED14 gene:
Search GeneIP for patents involving MED14

GeneCards and IP:
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MED14 Gene

mediator complex subunit 14

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MED14 (Gene Symbol)
Mediator Complex Subunit 14
CRSP2
CXorf4
EXLM1
TRAP170
RGR1
CRSP150
CSRP
Activator-Recruited Cofactor 150 KDa Component
Thyroid Hormone Receptor-Associated Protein Complex 170 KDa Component
Vitamin D3 Receptor-Interacting Protein Complex 150 KDa Component
ARC150
DRIP150
CRSP Complex Subunit 2
HRGR1
RGR1 Homolog
Cofactor Required For Sp1 Transcriptional Activation, Subunit 2, 150kDa
Cofactor Required For Sp1 Transcriptional Activation, Subunit 2 (150kD)
Human Homolog Of Yeast RGR1
Mediator Of RNA Polymerase II Transcription Subunit 14
Thyroid Hormone Receptor-Associated Protein Complex Component TRAP170
Transcriptional Co-Activator CRSP150
Vitamin D Receptor-Interacting Protein Complex Component DRIP150
Trap170
Cofactor Required For Sp1 Transcriptional Activation Subunit 2
Transcriptional Coactivator CRSP150

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MED14 Gene

mediator complex subunit 14

Aliasesfor MED14 gene

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