MED10 Gene
protein-coding GIFtS: 50
GCID: GC05M006428
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mediator complex subunit 10(Previous names: mediator of RNA polymerase II transcription, subunit 1...)
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Aliases
for MED10 gene
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc,
7Ensembl,
8DME,
9miRBase,
and/or 10fRNAdb)
About This Section
|
| Aliases |
|---|
| Mediator Complex Subunit 101 2 3 | | TRG-202 3 | | NUT21 2 5 | | L61 | | TRG201 2 | | MGC53091 | | Transformation-Related Gene 17 Protein2 3 | | Mediator Of RNA Polymerase II Transcription, Subunit 10 Homolog (NUT2, S.Cerevisiae)1 | | Transformation-Related Gene 20 Protein2 3 | | Mediator Of RNA Polymerase II Transcription Subunit 102 | | TRG-172 3 | | Mediator Of RNA Polymerase II Transcription, Subunit 102 |
Export aliases for MED10 gene to outside databases |
Summaries
for MED10 gene(According to Entrez Gene,
Tocris Bioscience,
Wikipedia's
Gene Wiki,
PharmGKB,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
|
Entrez Gene summary for MED10:
MED10 is a component of the Mediator complex, which is a coactivator for DNA-binding factors that activatetranscription via RNA polymerase II (Sato et al., 2003 (PubMed 12584197)).(supplied by OMIM, Oct 2008)
UniProtKB/Swiss-Prot: MED10_HUMAN, Q9BTT4Function: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNApolymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatoryproteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by directinteractions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complexwith RNA polymerase II and the general transcription factors
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Genomic Views
for MED10 gene
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 69),
Regulatory elements and Epigenetics data according to
QIAGEN,
SABiosciences, and/or
SwitchGear Genomics)
About This Section
| RefSeq DNA sequence:- NC_000005.9 NC_018916.1 NT_006576.16
Regulatory elements:
 SABiosciences Regulatory transcription factor binding sites in the MED10 gene promoter:
NRSF form 1 NRSF form 2 Tal-1beta E47 HEN1 AREB6 Nkx6-1 Zic3 ITF-2 ATF
Other transcription factors
Search SABiosciences Chromatin IP Primers for MED10
Epigenetics:
| | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat MED10 |
Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track
Entrez Gene cytogenetic band: 5p15.31 Ensembl cytogenetic band: 5p15.31 HGNC cytogenetic band: 5p15.31MED10 Gene in genomic location: bands according to Ensembl, locations according to
 (and/or Entrez Gene and/or Ensembl if different)
GeneLoc information about chromosome 5 GeneLoc Exon Structure GeneLoc location for GC05M006428: view genomic region
(about GC identifiers)
Start:
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6,371,994 bp from pter |
End:
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6,378,707 bp from pter |
Size:
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6,714 bases |
Orientation:
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minus strand |
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Proteins
for MED10 gene
(According to
1UniProtKB,
HORDE,
neXtProt,
Ensembl,
and/or Reactome,
Modification sites according to 2PhosphoSitePlus,
Specific Peptides from DME,
Protein expression images according to data from SPIRE MOPED and PaxDb,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from
EMD Millipore,
R&D Systems,
GenScript,
Enzo Life Sciences,
OriGene,
Novus Biologicals,
Sino Biological,
ProSpec, and/or
Uscn,
Biochemical Assays by
EMD Millipore,
R&D Systems,
OriGene,
GenScript,
Cell Signaling Technology,
Enzo Life Sciences, and/or
Uscn,
Ontologies according to Gene
Ontology Consortium 01 Mar 2013 and
Entrez Gene,
Antibodies by
EMD Millipore,
R&D Systems,
GenScript,
Cell Signaling Technology,
OriGene,
Novus Biologicals,
Thermo Fisher Scientific,
Abcam, and/or
Uscn)
About This Section
|
UniProtKB/Swiss-Prot: MED10_HUMAN, Q9BTT4 (See
protein sequence)Recommended Name: Mediator of RNA polymerase II transcription subunit 10 Size: 135 amino acids; 15688 Da
Subunit: Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11,MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26,MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinctmodule termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module insupporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinctsubunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP
Subcellular location: Nucleus (Probable)
Secondary accessions: C6G491Explore the universe of human proteins at neXtProt for MED10: NX_Q9BTT4
Post-translational modifications:
View modification sites using PhosphoSitePlus2View neXtProt modification sites for NX_Q9BTT4
MED10 Protein expression data from MOPED and PaxDb: About this image 
REFSEQ proteins: NP_115662.2
ENSEMBL proteins: ENSP00000255764 Reactome Protein details: Q9BTT4
Human Recombinant Protein Products:
Gene Ontology (GO): 2 cellular component terms (GO ID links to tree view): About this table
MED10 for ontologies About GeneDecksing
MED10 Antibody Products:
Assay Products for MED10: |
Protein
Domains /
Families
for MED10 gene(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
|
MED10 for domains About GeneDecksing
1 InterPro domain/family:Graphical View of Domain Structure for InterPro Entry Q9BTT4ProtoNet protein and cluster: Q9BTT4 UniProtKB/Swiss-Prot: MED10_HUMAN, Q9BTT4Similarity: Belongs to the Mediator complex subunit 10 family |
Function
for MED10 gene
(According to 1UniProtKB,
Genatlas,
LifeMap Discovery™,
IUBMB, and/or
2DME,
Human phenotypes from GenomeRNAi,
Animal models from MGI Mar 06 2013,
bound targets from SABiosciences,
miRNA Gene Targets from miRTarBase
shRNA from
OriGene,
RNAi from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
microRNA from QIAGEN,
Gene Editing from DNA2.0,
Clones from EMD Millipore,
OriGene,
SwitchGear Genomics,
GenScript,
Sino Biological,
DNA2.0,
and Vector BioLabs,
Cell Lines from GenScript,
LifeMap BioReagents,
In Situ Hybridization Assays from Advanced Cell Diagnostics,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene.)
About This Section
|
Function Summary:
UniProtKB/Swiss-Prot: MED10_HUMAN, Q9BTT4Function: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNApolymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatoryproteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by directinteractions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complexwith RNA polymerase II and the general transcription factors
Clone
Products: |  | Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore | |  | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for MED10 (see all 4)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for MED10
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling 
| |  | GenScript: all cDNA clones in your preferred vector: MED10 (NM_006827) | |  | Browse Sino Biological Human cDNA Clones | |  | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for MED10 | |  | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat MED10 |
Gene Ontology (GO): 1 molecular function term (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
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| GO:0001104 | RNA polymerase II transcription cofactor activity |
IEA | -- |
MED10 for ontologies About GeneDecksing
1 GenomeRNAi human phenotype for MED10:
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Pathways & Interactions
for MED10 gene
(Pathways according to
EMD Millipore,
R&D Systems,
Cell Signaling Technology,
KEGG,
PharmGKB,
BioSystems,
Reactome,
Tocris Bioscience,
GeneGo (Thomson Reuters),
QIAGEN,
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Interaction Networks according to
SABiosciences,
and/or STRING,
Interactions according to 1UniProtKB,
2MINT,
3I2D, and/or
4STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene).
About This Section
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Unified GeneCards pathways About this table
See pathways by source
| Super-pathway | contained gene-specific pathways |
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| 1 | Generic Transcription Pathway | | | 2 | HIV Infection | | | 3 | Transcriptional Regulation of White Adipocyte Differentiation | | | 4 | Axon guidance | |
Pathway sources
See GeneCards unified pathways
Show all pathways
1 EMD Millipore Pathway for MED10
1  GeneGo (Thomson Reuters) Pathway for MED10
4 
Reactome Pathways for MED10
MED10 for pathways About GeneDecksing
Interactions:
Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for MED10
STRING Interaction
Network Preview (showing 5 interactants - click image to see 25)
5/135 Interacting proteins for MED10 (Q9BTT42, 3 ENSP000002557644) via UniProtKB, MINT, STRING, and/or I2D (see all 135)About this table
Gene Ontology (GO): 4 biological process terms (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
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| GO:0006367 | transcription initiation from RNA polymerase II promoter |
TAS | -- | | GO:0010467 | gene expression |
TAS | -- | | GO:0019827 | stem cell maintenance |
IEA | -- | | GO:0045944 | positive regulation of transcription from RNA polymerase II promoter |
IEA | -- |
MED10 for ontologies About GeneDecksing
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Drugs & Compounds
for MED10 gene(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
EMD Millipore, Tocris Bioscience
HMDB,
BitterDB, and/or
Novoseek, and Drugs according to
DrugBank,
Enzo Life Sciences, and/or
PharmGKB, with drugs/clinical trials/news
search links to CenterWatch)
About This Section
|
 Browse Tocris compounds for MED10
Search CenterWatch for drugs/clinical trials and news about MED10
|
Transcripts
for MED10 gene(Secondary structures according to
fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
transcript ids from Ensembl
with links to UCSC,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
shRNA from
OriGene,
microRNA from QIAGEN,
Tagged/untagged cDNA clones from
OriGene,
SwitchGear Genomics,
GenScript,
DNA2.0,
Vector BioLabs,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN
)
About This Section
|
REFSEQ mRNAs for MED10 gene: NM_032286.2 Unigene Cluster for MED10: Mediator complex subunit 10 Hs.13885 [show with all ESTs]Unigene Representative Sequence: BG3351643 Ensembl transcripts including schematic representations, and UCSC links where relevant: ENST00000255764(uc003jdo.3) ENST00000503112 ENST00000504058
Clone
Products: | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for MED10 (see all 4)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for MED10
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling
| | | GenScript: all cDNA clones in your preferred vector: MED10 (NM_006827) | | | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for MED10 | | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat MED10 |
Additional cDNA sequence: AY277599.1 AY453397.1 AY598325.1 BC003353.1 7 DOTS entries: DT.101963980 DT.440550 DT.91682431 DT.100779959 DT.97808447 DT.100779960 DT.101966189 24/209 AceView cDNA sequences (see all 209): BQ448318 BU738047 AY598325 BQ002965 CA442315 BM312476 BU752461 BU684730 BM696608 NM_032286 AI762553 BE278138 AA460004 CA775315 CA444101 BX092103 AW082075 AK075493 AA648258 CA952401 CA441091 BF445457 BM314225 CB158002
GeneLoc Exon Structure
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Expression
for MED10 gene
(RNA expression data according to
H-InvDB,
NONCODE,
miRBase, and
RNAdb,
Expression images according to data from
BioGPS,
Illumina Human BodyMap, and
CGAP
SAGE,
Sets of similar genes according to GeneDecks,
in vivo and in vitro expression data from LifeMap Discovery™,
plus additional links to
Genevestigator, and/or
SOURCE, and/or
BioGPS, and/or
UniProtKB,
PCR Arrays from
SABiosciences,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN,
In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section
| MED10 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: CCTATAATAA
About this image
See MED10 Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for MED10
SOURCE GeneReport for Unigene cluster: Hs.13885
SABiosciences Custom PCR Arrays for MED10
Primer
Products: | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for MED10
Browse OriGene validated miRNA SYBR primer pairs
| | | SABiosciences RT2 qPCR Primer Assay in human, mouse / rat MED10 | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat MED10 | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat MED10 |
Orthologs
for MED10 gene
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
,
5MGI Mar 06 2013,
with possible further links to
Flybase
and/or
WormBase,
and/or
6Ensembl pan taxonomic compara ,
Gene Trees according to Ensembl and
TreeFam)
About This Section
|
This gene was present in the common ancestor of eukaryotes.
Orthologs for MED10 gene from 9/24 species (see all 24) About this table
ENSEMBL Gene Tree for MED10 (if available)
TreeFam Gene Tree for MED10 (if available) |
Paralogs
for MED10 gene(Paralogs according to
1HomoloGene,
2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
About This Section
| Paralogs for MED10 gene
MED10 for paralogs About GeneDecksing
1 Pseudogenes.org Pseudogene for MED10
PGOHUM00000233597
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Genomic Variants
for MED10 gene(SNPs/Variants according to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE,
UniProtKB, and
DNA2.0,
Linkage Disequilibrium by HapMap,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene
Mutation Database (HGMD) and the Locus Specific Mutation
Databases (LSDB), Blood group antigen gene mutations by BGMUT,
Resequencing Primers from QIAGEN,
Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section
|
| Genomic Data | Transcription Related Data | Allele Frequencies | | SNP ID | Valid | Clinical
significance | Chr 5 pos | Sequence | # | AA
Chg | Type | More | # | Allele
freq | Pop | Total
sample | More |
|---|
HapMap Linkage Disequilibrium report for MED10 (6371994 - 6378707 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV) variations for MED10: -- | SABiosciences Cancer Mutation PCR Assays |
| | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing MED10 |
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Disorders
/ Diseases
for MED10 gene
(in which this Gene is Involved, According to MalaCards,
OMIM, UniProtKB,
the University of Copenhagen DISEASES
database,
Genatlas, GeneTests,
GAD,
HuGE Navigator,
and/or TGDB.)
About This Section
|
MED10 for disorders About GeneDecksing
OMIM gene information: 612382
OMIM disorders: --
7  diseases for MED10: About MalaCardscri-du-chat syndrome junctional epidermolysis bullosa epidermolysis bullosa seborrheic keratosis
meningitis keratosis lung cancer
2 diseases from the University of Copenhagen DISEASES database for MED10:Junctional epidermolysis bullosa Seborrheic keratosis Export disorders for MED10 gene to outside databases
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Publications
for MED10 gene (in
PubMed.
Associations of this gene to articles via
1Entrez Gene,
2UniProtKB/Swiss-Prot,
3HGNC,
4GAD,
5PharmGKB,
6HMDB,
7DrugBank,
8UniProtKB/TrEMBL,
9 Novoseek, and/or
10fRNAdb)
About This Section
|
PubMed articles for MED10 gene, integrated from 9 sources (see all 24):
(articles sorted by number of sources associating them with MED10) | |  | Utopia: connect your pdf to the dynamic
world of online information |
- A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology. (PubMed id 15175163)1, 2, 3 Sato S....Conaway R.C. (2004)
- MED1/TRAP220 exists predominantly in a TRAP/Mediator subpopulation enriched in RNA polymerase II and is required for ER-mediated transcription. (PubMed id 15989967)1, 2 Zhang X....Roeder R.G. (2005)
- Determination of the 'critical region' for cat-like cry of Cri-du-chat syndrome and analysis of candidate genes by quantitative PCR. (PubMed id 15657623)1, 3 Wu Q....Hansen L. (2005)
- Suppression subtractive hybridization and expression profiling identifies a unique set of genes overexpressed in non-small-cell lung cancer. (PubMed id 15334068)1, 2 Petroziello J.... Carter P. (2004)
- A novel human SRB/MED-containing cofactor complex, SMCC, involved in transcription regulation. (PubMed id 10024883)1, 2 Gu W.... Roeder R.G. (1999)
- A census of human soluble protein complexes. (PubMed id 22939629)1 Havugimana P.C....Emili A. (2012)
- Initial characterization of the human central proteome. (PubMed id 21269460)2 Burkard T.R.... Colinge J. (2011)
- A proteome-wide, quantitative survey of in vivo ubiqui tylation sites reveals widespread regulatory roles. (PubMed id 21890473)1 Wagner S.A....Choudhary C. (2011)
- A directed protein interaction network for investigat ing intracellular signal transduction. (PubMed id 21900206)1 Vinayagam A....Wanker E.E. (2011)
- Mass spectrometric analysis of lysine ubiquitylation r eveals promiscuity at site level. (PubMed id 21139048)1 Danielsen J.M....Nielsen M.L. (2011)
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External Searches for MED10 gene
(in PubMed,
OMIM, and NCBI Bookshelf)
About This Section
|
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Genome Databases showing MED10 gene
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
Kegg,
and/or
H-InvDB)
About This Section
|
|
Other Databases showing MED10 gene
(According to HUGE)
About This Section
| -- |
Specialized Databases showing MED10 gene(According to PharmGKB,
ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
|
| Name | Description |
| PharmGKB entry for MED10 | Pharmacogenomics, SNPs, Pathways |
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| | |
About This Section
| Patent Information for MED10 gene:
Search GeneIP for patents involving MED10
GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
|
Products
for MED10 gene(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or
Enzo Life Sciences),
In Situ Hybridization Assays from
Advanced Cell Diagnostics
About This Section
|
 | |
 | |
 |
| | |  | Browse OriGene Antibodies | | OriGene shRNA RFP for MED10 | | | OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for MED10 | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for MED10 | | | OriGene Protein Over-expression Lysate for MED10 | | Browse OriGene Fluorogenic Cell Assay Kits | | | Browse OriGene siRNAs | | OriGene 3'-UTR Clone for MED10 | | | OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for MED10 | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for MED10 | | | Browse OriGene GFP tagged cDNA clones in CMV expression vector | | Browse OriGene MicroRNA Expression Plasmids | | | Browse OriGene basic RS shRNAs | | Browse OriGene validated miRNA SYBR primer pairs | | | OriGene Purified Protein for MED10 | | OriGene custom cloning services - gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling | | | OriGene Custom Antibody Services for MED10 | | OriGene Custom Protein Services for MED10 | | | OriGene Custom Immunoassay Development | | |
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| | | QIAGEN Custom miScript Target Protector blocks miRNA-binding site of in human, mouse, rat MED10 | | | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing MED10 | | | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat MED10 | | | QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat MED10 | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat MED10 | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat MED10 |
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| | | | Search Tocris compounds for MED10 |
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 | | | MED10 Proteins, Antibodies, CLIAs, and ELISAs |
| | | |  |  |  | | | | Search ThermoFisher Antibodies for MED10 |
| | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat MED10 |
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