Set Analyses:
Advanced Search

Advanced Search

 
Search By
Section (entire)
for


 



MCM8 Gene

protein-coding   GIFtS: 58
GCID: GC20P005926

Minichromosome Maintenance Complex Component 8

(Previous name: chromosome 20 open reading frame 154)
(Previous symbol: C20orf154)
Pneumococci & Pneumococcal Diseases Congress
  See related diseases
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
About This Section

TryGeneCards Plus

Aliases
Minichromosome Maintenance Complex Component 81 2     DNA Replication Licensing Factor MCM82
C20orf1541 2 3     MCM8 Minichromosome Maintenance Deficient 82
Chromosome 20 Open Reading Frame 1541     REC Homolog2
REC Homolog (Drosophila)1     EC 3.6.4.123
dJ967N21.52     Minichromosome Maintenance 83
DNA Helicase MCM82     

External Ids:    HGNC: 161471   Entrez Gene: 845152   Ensembl: ENSG000001258857   OMIM: 6081875   UniProtKB: Q9UJA33   

Export aliases for MCM8 gene to outside databases

Previous GC identifer: GC20P005879


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

TryGeneCards Plus

Entrez Gene summary for MCM8 Gene:
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that
are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the
mini-chromosome maintenance proteins is a key component of the pre-replication complex and may be involved in the
formation of replication forks and in the recruitment of other DNA replication related proteins. This protein
contains the central domain that is conserved among the mini-chromosome maintenance proteins. The encoded protein
may interact with other mini-chromosome maintenance proteins and play a role in DNA replication. This gene may be
associated with length of reproductive lifespan and menopause. Alternatively spliced transcript variants encoding
distinct isoforms have been described. (provided by RefSeq, Jul 2013)

GeneCards Summary for MCM8 Gene:
MCM8 (minichromosome maintenance complex component 8) is a protein-coding gene. Diseases associated with MCM8 include gingival recession, and cri-du-chat syndrome. GO annotations related to this gene include helicase activity. An important paralog of this gene is MCM4.

UniProtKB/Swiss-Prot: MCM8_HUMAN, Q9UJA3
Function: Component of the MCM8-MCM9 complex, a complex involved in homologous recombination repair following DNA
interstrand cross-links and plays a key role during gametogenesis. The MCM8-MCM9 complex probably acts as a
hexameric helicase downstream of the Fanconi anemia proteins BRCA2 and RAD51 and is required to process aberrant
forks into homologous recombination substrates and to orchestrate homologous recombination with resection, fork
stabilization and fork restart. May also play a non-essential for DNA replication: may be involved in the
activation of the prereplicative complex (pre-RC) during G(1) phase by recruiting CDC6 to the origin recognition
complex (ORC). Binds chromatin throughout the cell cycle

Gene Wiki entry for MCM8 Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
About This Section

TryGeneCards Plus
RefSeq DNA sequence:
NC_000020.11  NT_011387.9  NC_018931.2  
Regulatory elements:
   Regulatory transcription factor binding sites in the MCM8 gene promoter:
         RFX1   p53   AML1a   HOXA3   POU3F1   POU3F2 (N-Oct-5b)   POU3F2 (N-Oct-5a)   POU3F2   c-Myb   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidMCM8 promoter sequence
   Search Chromatin IP Primers for MCM8

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat MCM8


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 20p12.3   Ensembl cytogenetic band:  20p12.3   HGNC cytogenetic band: 20p12.3

MCM8 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
MCM8 gene location

GeneLoc information about chromosome 20         GeneLoc Exon Structure

GeneLoc location for GC20P005926:  view genomic region     (about GC identifiers)

Start:
5,931,298 bp from pter      End:
5,975,852 bp from pter
Size:
44,555 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Cloud-Clone Corp.,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Cloud-Clone Corp., Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, and/or Cloud-Clone Corp.)
About This Section

TryGeneCards Plus

UniProtKB/Swiss-Prot: MCM8_HUMAN, Q9UJA3 (See protein sequence)
Recommended Name: DNA helicase MCM8  
Size: 840 amino acids; 93697 Da
Subunit: Component of the MCM8-MCM9 complex, which forms a hexamer composed of MCM8 and MCM9. Interacts with CDC6
and ORC2
Caution: Was initially thought to play a role in DNA replication (PubMed:15684404). However, it was later shown
that it is mainly involved in homologous recombination repair (PubMed:22771115)
Sequence caution: Sequence=BAB55260.1; Type=Erroneous initiation; Note=Translation N-terminally extended;
Secondary accessions: B2RBG7 D3DW08 E7EQU7 Q495R4 Q495R6 Q495R7 Q86US4 Q969I5
Alternative splicing: 4 isoforms:  Q9UJA3-1   Q9UJA3-2   Q9UJA3-3   Q9UJA3-4   (No experimental confirmation available)

Explore the universe of human proteins at neXtProt for MCM8: NX_Q9UJA3

Explore proteomics data for MCM8 at MOPED

Post-translational modifications: 

  • Modification sites at neXtProt
  • Modification sites at PhosphoSitePlus

  • See MCM8 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins (5 alternative transcripts): 
    NP_001268449.1  NP_001268450.1  NP_001268451.1  NP_115874.3  NP_877954.1  

    ENSEMBL proteins: 
     ENSP00000368174   ENSP00000265187   ENSP00000368161   ENSP00000368164  
    Reactome Protein details: Q9UJA3

    MCM8 Human Recombinant Protein Products:

    Browse Purified and Recombinant Proteins at EMD Millipore
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Browse OriGene full length recombinant human proteins expressed in human HEK293 cells
    Browse OriGene Protein Over-expression Lysates
    OriGene Custom MassSpec
    OriGene Custom Protein Services for MCM8
    GenScript Custom Purified and Recombinant Proteins Services for MCM8
    Novus Biologicals MCM8 Protein
    Novus Biologicals MCM8 Lysate
    Browse Sino Biological Recombinant Proteins
    Browse Sino Biological Cell Lysates
    Browse ProSpec Recombinant Proteins
    Cloud-Clone Corp. Proteins for MCM8

    MCM8 Antibody Products:

    Browse EMD Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Browse R&D Systems for Antibodies
    OriGene Antibodies for MCM8
    OriGene Custom Antibody Services for MCM8
    Novus Biologicals MCM8 Antibodies
    Abcam antibodies for MCM8
    Cloud-Clone Corp. Antibodies for MCM8
    ThermoFisher Antibodies for MCM8
    LSBio Antibodies in human, mouse, rat for MCM8

    MCM8 Assay Products:

    Browse Kits and Assays available from EMD Millipore
    OriGene Custom Assay Services for MCM8
    Browse R&D Systems for biochemical assays
    GenScript Custom Assay Services for MCM8
    Browse Enzo Life Sciences for kits & assays
    Cloud-Clone Corp. ELISAs for MCM8
    Cloud-Clone Corp. CLIAs for MCM8


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    TryGeneCards Plus
    5 InterPro protein domains:
     IPR027925 MCM_N
     IPR012340 NA-bd_OB-fold
     IPR027417 P-loop_NTPase
     IPR003593 AAA+_ATPase
     IPR001208 MCM_DNA-dep_ATPase

    Graphical View of Domain Structure for InterPro Entry Q9UJA3

    ProtoNet protein and cluster: Q9UJA3

    1 Blocks protein domain: IPB001208 MCM family

    UniProtKB/Swiss-Prot: MCM8_HUMAN, Q9UJA3
    Similarity: Belongs to the MCM family
    Similarity: Contains 1 MCM domain


    MCM8 for domains           About GeneDecksing


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
    About This Section

    TryGeneCards Plus

    Molecular Function:

         UniProtKB/Swiss-Prot Summary: MCM8_HUMAN, Q9UJA3
    Function: Component of the MCM8-MCM9 complex, a complex involved in homologous recombination repair following DNA
    interstrand cross-links and plays a key role during gametogenesis. The MCM8-MCM9 complex probably acts as a
    hexameric helicase downstream of the Fanconi anemia proteins BRCA2 and RAD51 and is required to process aberrant
    forks into homologous recombination substrates and to orchestrate homologous recombination with resection, fork
    stabilization and fork restart. May also play a non-essential for DNA replication: may be involved in the
    activation of the prereplicative complex (pre-RC) during G(1) phase by recruiting CDC6 to the origin recognition
    complex (ORC). Binds chromatin throughout the cell cycle
    Catalytic activity: ATP + H(2)O = ADP + phosphate
    Induction: By E2F1

         Enzyme Number (IUBMB): EC 3.6.4.121

         Gene Ontology (GO): Selected molecular function terms (see all 8):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000166nucleotide binding ----
    GO:0003677DNA binding IEA--
    GO:0004386helicase activity IEA--
    GO:0005515protein binding IPI--
    GO:0005524ATP binding IEA--
         
    MCM8 for ontologies           About GeneDecksing


    Phenotypes:
         5 MGI mutant phenotypes (inferred from 1 allele(MGI details for Mcm8):
     cellular  endocrine/exocrine gland  homeostasis/metabolism  reproductive system  tumorigenesis 

    MCM8 for phenotypes           About GeneDecksing

    Animal Models:
         MGI mouse knock-out Mcm8tm1.1Geno for MCM8

       inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for MCM8
       inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for MCM8

       genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for MCM8
       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for MCM8

    miRNA
    Products:
        
    miRTarBase miRNAs that target MCM8:
    hsa-mir-155-5p (MIRT020992), hsa-mir-99a-5p (MIRT048686), hsa-mir-193b-3p (MIRT016553)

    Block miRNA regulation of human, mouse, rat MCM8 using miScript Target Protectors
    4 qRT-PCR Assays for microRNAs that regulate MCM8:
    hsa-miR-135b hsa-miR-3919 hsa-miR-3925-5p hsa-miR-135a
    SwitchGear 3'UTR luciferase reporter plasmidMCM8 3' UTR sequence
    Inhib. RNA
    Products:
        
    OriGene RNAi products in human, mouse, rat for MCM8
    Predesigned siRNA for gene silencing in human, mouse, rat MCM8

    Gene Editing
    Products:
    DNA2.0 Custom Protein Engineering Service for MCM8

    Clone
    Products:
         
    OriGene clones in human, mouse for MCM8 (see all 15)
    OriGene ORF clones in mouse, rat for MCM8
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector (see all 2): MCM8 (NM_032485)
    Sino Biological Human cDNA Clone for MCM8
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for MCM8
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat MCM8

    Cell Line
    Products:
         
    GenScript Custom overexpressing Cell Line Services for MCM8
    Browse ESI BIO Cell Lines and PureStem Progenitors for MCM8 
    In Situ Assay
    Products:
       

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for MCM8


    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
    About This Section

    TryGeneCards Plus

    Subcellular locations from UniProtKB/Swiss-Prot
    MCM8_HUMAN, Q9UJA3: Nucleus. Note=Localizes to nuclear foci and colocalizes with RAD51
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    nucleus5
    extracellular2
    cytoskeleton1
    cytosol1
    mitochondrion1
    plasma membrane1

    Gene Ontology (GO): 3 cellular component terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634nucleus IDA--
    GO:0005654nucleoplasm TAS--
    GO:0097362MCM8-MCM9 complex IDA--

    MCM8 for ontologies           About GeneDecksing


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
    About This Section

    TryGeneCards Plus

    SuperPaths for MCM8 About   (see all 8)                                                                                              See pathways by source

    SuperPathSelected contained pathways About (see all per SuperPath)
    1CDK-mediated phosphorylation and removal of Cdc6
    Switching of origins to a post-replicative state0.93
    Removal of licensing factors from origins0.86
    Orc1 removal from chromatin0.93
    M/G1 Transition0.81
    Regulation of DNA replication0.93
    DNA Replication Pre-Initiation0.81
    Synthesis of DNA0.92
    S Phase0.78
    2E2F mediated regulation of DNA replication
    G1/S Transition0.81
    E2F mediated regulation of DNA replication0.30
    Mitotic G1-G1/S phases0.81
    E2F-enabled inhibition of pre-replication complex formation0.00
    3Cell Cycle, Mitotic
    Cell Cycle, Mitotic0.90
    Cell Cycle0.90
    4G2/M Checkpoints
    G2/M Checkpoints0.84
    Activation of ATR in response to replication stress0.84
    5DNA strand elongation
    DNA strand elongation0.63
    Unwinding of DNA0.00

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways

    1 Downloadable PowerPoint Slide of GeneGlobe Pathway Central Maps for MCM8
        Cell Cycle Control of Chromosomal Replication

    1 BioSystems Pathway for MCM8
        Cell cycle


    Selected Reactome Pathways for MCM8 (see all 9)
        Unwinding of DNA
    Activation of ATR in response to replication stress
    Removal of licensing factors from origins
    E2F-enabled inhibition of pre-replication complex formation
    Orc1 removal from chromatin



    MCM8 for pathways           About GeneDecksing

        Custom Pathway & Disease-focused RT2 Profiler PCR Arrays for MCM8
    Interactions:

        GeneGlobe Interaction Network for MCM8

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    Selected Interacting proteins for MCM8 (Q9UJA33 ENSP000003681744) via UniProtKB, MINT, STRING, and/or I2D (see all 78)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    MCM4P339913, ENSP000002621054I2D: score=2 STRING: ENSP00000262105
    CDC6Q997413, ENSP000002097284I2D: score=1 STRING: ENSP00000209728
    ORC2Q134163, ENSP000002342964I2D: score=1 STRING: ENSP00000234296
    MCM6Q145663, ENSP000002641564I2D: score=2 STRING: ENSP00000264156
    MCM7P339933, ENSP000003072884I2D: score=2 STRING: ENSP00000307288
    About this table

    Gene Ontology (GO): Selected biological process terms (see all 10):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000082G1/S transition of mitotic cell cycle TAS--
    GO:0000278mitotic cell cycle TAS--
    GO:0000724double-strand break repair via homologous recombination IEA--
    GO:0006260DNA replication TAS--
    GO:0006271DNA strand elongation involved in DNA replication TAS--

    MCM8 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
    About This Section

    TryGeneCards Plus
    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Browse Tocris compounds for MCM8



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    Conferences by KenesGroup, exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, and/or QIAGEN )
    About This Section

    TryGeneCards Plus

    REFSEQ mRNAs for MCM8 gene (5 alternative transcripts): 
    NM_001281520.1  NM_001281521.1  NM_001281522.1  NM_032485.5  NM_182802.2  

    Unigene Cluster for MCM8:

    Minichromosome maintenance complex component 8
    Hs.597484  [show with all ESTs]
    Unigene Representative Sequence: NM_182802
    4 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000378896(uc002wmi.3 uc002wmj.3 uc002wmk.3 uc002wml.3 uc010gbp.3)
    ENST00000265187(uc002wmm.3) ENST00000378883 ENST00000378886

    miRNA
    Products:
         
    Block miRNA regulation of human, mouse, rat MCM8 using miScript Target Protectors
    4 qRT-PCR Assays for microRNAs that regulate MCM8:
    hsa-miR-135b hsa-miR-3919 hsa-miR-3925-5p hsa-miR-135a
    SwitchGear 3'UTR luciferase reporter plasmidMCM8 3' UTR sequence
    Inhib. RNA
    Products:
         
    OriGene RNAi products in human, mouse, rat for MCM8
    Predesigned siRNA for gene silencing in human, mouse, rat MCM8
    Clone
    Products:
         
    OriGene clones in human, mouse for MCM8 (see all 15)
    OriGene ORF clones in mouse, rat for MCM8
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector (see all 2): MCM8 (NM_032485)
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for MCM8
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat MCM8
    Primer
    Products:
        
    OriGene qSTAR qPCR primer pairs in human, mouse for MCM8
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat MCM8
      QuantiTect SYBR Green Assays in human, mouse, rat MCM8
      QuantiFast Probe-based Assays in human, mouse, rat MCM8

    Additional mRNA sequence: 

    AJ439063.1 AK027644.1 AK297806.1 AK314654.1 AY158211.1 BC005170.1 BC008830.2 BC080656.1 
    BC101054.1 BC101055.1 BC101056.1 BC101057.1 

    6 DOTS entries:

    DT.100778920  DT.112513  DT.91762513  DT.99984722  DT.120833354  DT.91875952 

    Selected AceView cDNA sequences (see all 124):

    BM810194 AI032684 BU509003 AW963925 BX106269 AI913762 AA379359 AA563920 
    AI081701 BQ441929 AK022738 BQ007416 AU125903 AA609373 BF509125 BQ049652 
    AI078438 R10261 BM558689 BX282225 BX504348 AA370141 BU162199 AA193063 

    GeneLoc Exon Structure

    Selected Alternative Splicing Database (ASD) splice patterns (SP) for MCM8 (see all 7)    About this scheme

    ExUns: 1a · 1b · 1c · 1d · 1e ^ 2 ^ 3 ^ 4 ^ 5 ^ 6 ^ 7 ^ 8 ^ 9 ^ 10a · 10b ^ 11 ^ 12 ^ 13 ^ 14 ^ 15 ^ 16 ^ 17 ^ 18a · 18b ^ 19 ^ 20 ^
    SP1:                          -                                                                       -           -                                             
    SP2:                          -                                                                 -                 -                                             
    SP3:                                                                                                  -           -                                             
    SP4:                                                                                -                 -           -                                             
    SP5:                          -                                                                       -     -     -                                             

    ExUns: 21a · 21b
    SP1:            
    SP2:            
    SP3:            
    SP4:            
    SP5:            


    ECgene alternative splicing isoforms for MCM8

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    TryGeneCards Plus

    MCM8 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: GTTCTGGAAT
    MCM8 Expression
    About this image

    MCM8 Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    MCM8 Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.597484

    UniProtKB/Swiss-Prot: MCM8_HUMAN, Q9UJA3
    Tissue specificity: Highest levels in placenta, lung and pancreas. Low levels in skeletal muscle and kidney.
    Expressed in various tumors with highest levels in colon and lung cancers

        Custom PCR Arrays for MCM8
    Primer
    Products:
    OriGene qSTAR qPCR primer pairs in human, mouse for MCM8
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat MCM8
    QuantiTect SYBR Green Assays in human, mouse, rat MCM8
    QuantiFast Probe-based Assays in human, mouse, rat MCM8
    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for MCM8

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    TryGeneCards Plus

    This gene was present in the common ancestor of eukaryotes.

    Orthologs for MCM8 gene from Selected species (see all 20)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Mcm81 , 5 minichromosome maintenance deficient 8 (S. cerevisiae)1, 5 87.12(n)1
    90.31(a)1
      2 (64.75 cM)5
    666341  NM_025676.31  NP_079952.21 
     1328161415 
    chicken
    (Gallus gallus)
    Aves MCM81 minichromosome maintenance complex component 8 74.77(n)
    79.75(a)
      421314  XM_004935291.1  XP_004935348.1 
    lizard
    (Anolis carolinensis)
    Reptilia MCM86
    minichromosome maintenance complex component 8
    79(a)
    1 ↔ 1
    1(129363630-129387076)
    African clawed frog
    (Xenopus laevis)
    Amphibia Xl.224882 Xenopus laevis transcribed sequence with weak similarity more 73.9(n)    48037082 
    zebrafish
    (Danio rerio)
    Actinopterygii 570619252   -- 74.47(n)    57061925 
    fruit fly
    (Drosophila melanogaster)
    Insecta rec6
    recombination-defective
    26(a)
    1 ↔ 1
    3R(11648741-11658354)
    baker's yeast
    (Saccharomyces cerevisiae)
    Saccharomycetes MCM46
    Essential helicase component of heterohexameric MC...
    22(a)
    1 → many
    XVI(596750-599551) YPR019W
    thale cress
    (Arabidopsis thaliana)
    eudicotyledons MCM81 MCM8 54.58(n)
    49.25(a)
      820123  NM_111800.2  NP_187577.1 
    rice
    (Oryza sativa)
    Liliopsida Os05g04641001 Os05g0464100 55.04(n)
    49.61(a)
      4339038  NM_001062312.1  NP_001055777.1 


    ENSEMBL Gene Tree for MCM8 (if available)
    TreeFam Gene Tree for MCM8 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section

    TryGeneCards Plus
    Paralogs for MCM8 gene
    MCM42  
    1 SIMAP similar gene for MCM8 using alignment to 1 protein entry:     MCM8_HUMAN:
    FLJ00323

    MCM8 for paralogs           About GeneDecksing



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
    About This Section

    TryGeneCards Plus

    Selected SNPs for MCM8 (see all 1072)    About this table    
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 20 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs114722101,2
    C--5922827(+) GACAG-/ACACACA 2 -- ut310--------
    rs2007636551,2
    --5933179(+) GGTAGA/GGTTTA 2 -- int10--------
    rs1882075351,2
    C--5933185(+) GTTTAA/GAAAGT 2 -- int10--------
    rs1445138501,2
    C--5933334(+) GCATTG/TTAGAC 2 -- int10--------
    rs1466283461,2
    --5933345(+) TTTATA/GTCAGT 2 -- int10--------
    rs738940941,2
    C,F--5933352(+) CAGTCA/GGGAAA 2 -- int14Minor allele frequency- G:0.09WA CSA 126
    rs759882471,2
    C--5933454(+) GAGGTA/TACTCC 2 -- int12Minor allele frequency- T:0.28CSA EA 122
    rs60768961,2
    H--5933494(+) ccatcC/Tttatg 2 -- int14Minor allele frequency- T:0.00NS EA 416
    rs2361111,2
    C,F,H--5933535(-) CCACAT/CTCTGG 2 -- int119Minor allele frequency- C:0.10NS EA NA WA 2344
    rs1810004771,2
    --5933551(+) CAAAGG/TTTGGA 2 -- int10--------

    HapMap Linkage Disequilibrium report for MCM8 (5931298 - 5975852 bp)

    Structural Variations
         Database of Genomic Variants (DGV) 6 variations for MCM8:    About this table    
    Variant IDTypeSubtypePubMed ID
    esv2722144CNV Deletion23290073
    esv2244525CNV Deletion18987734
    esv2453476CNV Deletion19546169
    esv1746393CNV Deletion17803354
    nsv179031CNV Loss16902084
    esv7029CNV Loss19470904

    Human Gene Mutation Database (HGMD): MCM8
    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing MCM8
    DNA2.0 Custom Variant and Variant Library Synthesis for MCM8

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Conferences by KenesGroup, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    TryGeneCards Plus
    OMIM gene information: 608187    OMIM disorders: --

    14 diseases for MCM8:    About MalaCards
    gingival recession    cri-du-chat syndrome    wolf-hirschhorn syndrome    gingivitis
    microcephaly    fanconi's anemia    pre-eclampsia    eclampsia
    hyperglycemia    periodontitis    neuroblastoma    lung cancer
    hiv-1    malaria

    1 disease from the University of Copenhagen DISEASES database for MCM8:
    Gingival recession

    MCM8 for disorders           About GeneDecksing


    Congresses - knowledge worth sharing:
    The International Symposium on Pneumococci and Pneumococcal Diseases (ISPPD) 9 - 13 March 2014
    Genetic Association Database (GAD): MCM8
    Human Genome Epidemiology (HuGE) Navigator: MCM8 (6 documents)

    Export disorders for MCM8 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    TryGeneCards Plus

    PubMed articles for MCM8 gene, integrated from 10 sources (see all 32):
    (articles sorted by number of sources associating them with MCM8)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Identification and functional characterization of a new member of the human Mcm protein family: hMcm8. (PubMed id 12527764)1, 2, 3 Gozuacik D.... Paterlini-Brechot P. (Nucleic Acids Res. 2003)
    2. A new member of the MCM protein family encoded by the human MCM8 gene, located contrapodal to GCD10 at chromosome band 20p12.3-13. (PubMed id 12771218)1, 2, 9 Johnson E.M.... Daniel D.C. (Nucleic Acids Res. 2003)
    3. Involvement of human MCM8 in prereplication complex assembly by recruiting hcdc6 to chromatin. (PubMed id 15684404)1, 2, 9 Volkening M. and Hoffmann I. (Mol. Cell. Biol. 2005)
    4. Meta-analyses identify 13 loci associated with age at menopause and highlight DNA repair and immune pathways. (PubMed id 22267201)1, 4 Stolk L....Lunetta K.L. (Nat. Genet. 2012)
    5. Host determinants of HIV-1 control in African Americans. (PubMed id 20205591)1, 4 Pelak K.... . (J. Infect. Dis. 2010)
    6. Investigation of genetic susceptibility factors for human longevity - a targeted nonsynonymous SNP study. (PubMed id 20800603)1, 4 Flachsbart F....Nebel A. (Mutat. Res. 2010)
    7. Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study. (PubMed id 20628086)1, 4 Bailey S.D....Anand S. (Diabetes Care 2010)
    8. Loci at chromosomes 13, 19 and 20 influence age at natural menopause. (PubMed id 19448619)1, 4 Stolk L....Uitterlinden A.G. (Nat. Genet. 2009)
    9. Genome-wide association studies identify loci associated with age at menarche and age at natural menopause. (PubMed id 19448621)1, 4 He C....Chasman D.I. (Nat. Genet. 2009)
    10. Colocalization of MCM8 and MCM7 with proteins involved in distinct aspects of DNA replication. (PubMed id 18072282)1, 2 Kinoshita Y.... Daniel D.C. (Microsc. Res. Tech. 2008)

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

    TryGeneCards Plus
     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section

    TryGeneCards Plus
    Entrez Gene: 84515 HGNC: 16147 AceView: MCM8 Ensembl:ENSG00000125885 euGenes: HUgn84515
    ECgene: MCM8 H-InvDB: MCM8

    (According to HUGE)
    About This Section

    TryGeneCards Plus
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
    About This Section

    TryGeneCards Plus
    NameDescription
    PharmGKB entry for MCM8 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section

    TryGeneCards Plus
    Patent Information for MCM8 gene:
    Search GeneIP for patents involving MCM8

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript, and ESI BIO, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
    Advanced Cell Diagnostics, Animal models from inGenious Targeting Laboratory, genOway)
    About This Section

    TryGeneCards Plus

     
     EMD Millipore genomic analysis products

      
     Browse Antibodies   Browse Cell Culture Products  
     Browse ELISAs   Browse Flow Cytometry Kits  
     Browse Primer Pairs   Browse Enzyme Activity Assays/Reagents  
     Browse ELISpot/FluoroSpot Kits/Development Modules   Browse TFB/Immunoprecipitation Assays  
     Browse Apoptosis Detection Kits/Reagents   Browse Ubiquitin Proteasome Pathway (UPP) Assay Kits/Reagents  
     Browse DNA Damage/Repair Kits/Reagents   Browse Luminex Assays  
     Browse Cell Selection/Detection Kits/Reagents   Browse Secondary Antibodies/Controls/Staining Reagents  
     Browse Recombinant/Natural Proteins   Browse Stem Cell Products  
     Browse cDNA Clones   Browse Proteome Profiler Antibody Arrays  
     OriGene Antibodies for MCM8   OriGene RNAi products in human, mouse, rat for MCM8  
     Browse OriGene qPCR primer pairs and template standards   Browse OriGene Protein Over-expression Lysates  
     OriGene Custom Mass Spec   OriGene clones in human, mouse for MCM8  
     OriGene qSTAR qPCR primer pairs in human, mouse for MCM8   Browse OriGene full length recombinant human proteins expressed in human HEK293 cells  
     OriGene ORF clones in mouse, rat for MCM8   OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling  
     OriGene Custom Antibody Services for MCM8   OriGene Custom Protein Services for MCM8  

     
     
     Block miRNA regulation of human, mouse, rat MCM8 using miScript Target Protectors SeqTarget long-range PCR primers for resequencing MCM8
     DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat MCM8 Predesigned siRNA for gene silencing in human, mouse, rat MCM8
     QuantiFast Probe-based Assays in human, mouse, rat MCM8 QuantiTect SYBR Green Assays in human, mouse, rat MCM8
     Custom PCR Arrays for MCM8 Search Chromatin IP Primers for MCM8
     Pre-validated RT2 qPCR Primer Assay in human, mouse, rat MCM8  GeneGlobe Interaction Network for MCM8
     Regulatory tfbs in MCM8 promoter
     GenScript Custom Purified and Recombinant Proteins Services for MCM8 GenScript cDNA clones with any tag delivered in your preferred vector for MCM8
     GenScript Custom Assay Services for MCM8 GenScript Custom overexpressing Cell Line Services for MCM8
     CloneReady with Over 120,000 Genes  Gene Synthesis: Any Gene in Any Vector
     Vector-based siRNA and miRNA, Ready for Transfection Gene Mutant Library, Variants up to 10^11
     Plasmid Preparation Custom Peptide Services
     Search for Antibodies & Assays

     Search Tocris compounds for MCM8
     Browse Sino Biological Proteins
     Browse Sino Biological Cell Lysates
     cDNA Clones for MCM8
     4000+ Proteins
     Search Sino Biological for antibodies, proteins & pathways
     Protein Production Services
     Transfection Reagents
     Protein A/G/L resins
     Isotyping reagents
     Search www.enzolifesciences.com for proteins, assays, substrates, inhibitors & antibodies

     Novus Tissue Slides
     MCM8 antibodies
     MCM8 proteins
     MCM8 lysates
     Antibodies for MCM8
     See all of Abcam's Antibodies, Kits and Proteins for MCM8
     Custom Antibody / Protein Production Service
     Bulk Purchasing
     Advantages of Rabbit Monoclonal antibodies
     Abcam protocols and scientific support
     Browse ProSpec Recombinant Proteins
     Proteins for MCM8
     Antibodies for MCM8
     ELISAs for MCM8
     CLIAs for MCM8



     Browse ESI BIO Cell Lines and PureStem Progenitors for MCM8
     Gene Synthesis
     Protein Engineering
     Variant Library Synthesis
     Codon Optimization
     Protein Production and Purification
     Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for MCM8
     SwitchGear 3'UTR luciferase reporter plasmids for MCM8
     SwitchGear Promoter luciferase reporter plasmids for MCM8
     ThermoFisher Antibodies for MCM8
     Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat MCM8
     inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for MCM8
     inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for MCM8
     LSBio Antibodies in human, mouse, rat for MCM8
    Customized transgenic rodents for:
     Humanization
     Biomarker expression
     Off-target effect monitoring
     Translational medicine
     Tissue-specific gene expresssion
     Time-controlled gene expresssion
     Browse compounds at ApexBio
           
    GeneCards Homepage - Last full update: 7 May 2014 - Incrementals: 9 May 2014 , 2 Jun 2014 , 26 Jun 2014 , 30 Jun 2014

    View Random Gene

    Category
    (GIFtS: )
    GIFtS Group
    The GeneCards human gene database gene index: 1 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 


    Developed at the Crown Human Genome Center, Department of Molecular Genetics, the Weizmann Institute of Science

    Hot genes      Disease genes      MCM8 gene at Home site.
    Version: 3.12.136 22 July 2014
    hostname: 356977-web1.xennexinc.com index build: 126 solr: 1.4