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Category Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21

GIFtS
-

MCM4 Gene

protein-coding GIFtS: 66
GCID: GC08P048873

minichromosome maintenance complex component 4

(Previous name: MCM4 minichromosome maintenance deficient 4 (S. cerevisiae)...)
(Previous symbol: CDC21)

Explore 12 diseases affiliated with
MCM4 via

MalaCards

our new
Human Malady Compendium

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc, ⁷Ensembl, ⁸DME, ⁹miRBase, and/or ¹⁰fRNAdb)
About This Section

Aliases
Minichromosome Maintenance Complex Component 4¹ ²		MCM4 Minichromosome Maintenance Deficient 4 (S. Cerevisiae)¹
CDC21¹ ² ³		NKCD²
CDC54¹ ²		NKGCD²
HCdc21¹		DNA Replication Licensing Factor MCM4²
P1-CDC21² ³		Homolog Of S. Pombe Cell Devision Cycle 21²
CDC21 Homolog² ³		Minichromosome Maintenance Deficient 4²
MGC33310¹		EC 3.6.4.12³
P1-Cdc21¹

External Ids:	HGNC: 6947¹	Entrez Gene: 4173²	Ensembl: ENSG00000104738⁷	OMIM: 602638⁵	UniProtKB: P33991³

Export aliases for MCM4 gene to outside databases

Previous GC identifers: GC08P047965 GC08P048877 GC08P048596 GC08P048923 GC08P049036 GC08P044336

(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for MCM4:

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are

essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is

a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and

in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6

and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this

protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped

to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the

repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been

reported. (provided by RefSeq, Jul 2008)

UniProtKB/Swiss-Prot: MCM4_HUMAN, P33991

Function: Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential

for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in

the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure

of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the

adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex

helicase activity

Gene Wiki entry for MCM4

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section

RefSeq DNA sequence:

NC_000008.10 NC_018919.1 NT_008183.19

Regulatory elements:

SABiosciences Regulatory transcription factor binding sites in the MCM4 gene promoter:
Nkx3-1 C/EBPbeta Nkx3-1 v4 p53 POU3F2 (N-Oct-5b) Nkx3-1 v1 Nkx3-1 v2 Nkx3-1 v3
Other transcription factors

SwitchGear Promoter luciferase reporter plasmid: MCM4 promoter sequence

Search SABiosciences Chromatin IP Primers for MCM4

Epigenetics:

QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat MCM4

Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 8q11.2 Ensembl cytogenetic band: 8q11.21 HGNC cytogenetic band: 8q12-q13

MCM4 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
MCM4 gene location

GeneLoc information about chromosome 8 GeneLoc Exon Structure

GeneLoc location for GC08P048873: view genomic region (about GC identifiers)

Start:	48,872,745 bp from pter	End:	48,890,720 bp from pter
Size:	17,976 bases	Orientation:	plus strand

(According to ¹UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to ²PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: MCM4_HUMAN, P33991 (See protein sequence)

Recommended Name: DNA replication licensing factor MCM4

Size: 863 amino acids; 96558 Da

Subunit: Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order

MCM2-MCM6-MCM4-MCM7-MCM3-MCM5 (By simililarity). Interacts with MCMBP

Subcellular location: Nucleus (By similarity)

Miscellaneous: Early fractionation of eukaryotic MCM proteins yielded a variety of dimeric, trimeric and tetrameric

complexes with unclear biological significance. Specifically a MCM467 subcomplex is shown to have in vitro helicase

activity which is inhibited by the MCM2 subunit. The MCM2-7 hexamer is the proposed physiological active complex

Secondary accessions: Q8NEH1 Q99658

Explore the universe of human proteins at neXtProt for MCM4: NX_P33991

Post-translational modifications:

View modification sites using PhosphoSitePlus²

View neXtProt modification sites for NX_P33991

MCM4 Protein expression data from MOPED and PaxDb: About this image

REFSEQ proteins (2 alternative transcripts):

NP_005905.2 NP_877423.1

ENSEMBL proteins:

ENSP00000430329 ENSP00000430194 ENSP00000262105 ENSP00000380038 ENSP00000427829

ENSP00000428833 ENSP00000427875 ENSP00000430864 ENSP00000429502 ENSP00000431011

Reactome Protein details: P33991
Human Recombinant Protein Products for MCM4:

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	OriGene Purified Proteins (see all 2): MCM4 OriGene Protein Over-expression Lysate (see all 2): MCM4 OriGene Custom Protein Services for MCM4
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	Novus Biologicals MCM4 Proteins Novus Biologicals MCM4 Lysates
	Browse Sino Biological Recombinant Proteins
	Browse ProSpec Recombinant Proteins
	Uscn Proteins for MCM4

Gene Ontology (GO): 4 cellular component terms (GO ID links to tree view): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005634	nucleus	NAS	8265339
GO:0005654	nucleoplasm	TAS	--
GO:0005730	NOT nucleolus	IDA	--
GO:0042555	MCM complex	IDA	17296731

MCM4 for ontologies About GeneDecksing

MCM4 Antibody Products:

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	Novus Biologicals MCM4 Antibodies
	Abcam antibodies for MCM4
	Uscn Antibodies for MCM4
	ThermoFisher Antibodies for MCM4

Assay Products for MCM4:

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	Browse R&D Systems for biochemical assays
	GenScript Custom Assay Services for MCM4
	Browse Enzo Life Sciences for kits & assays
	Uscn ELISAs and CLIAs for MCM4

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

MCM4 for domains About GeneDecksing

5/6 InterPro domains/families (see all 6):

IPR018525 MCM_CS

IPR012340 NA-bd_OB-fold

IPR004039 Rubredoxin-type_fold

IPR003593 AAA+_ATPase

IPR001208 MCM_DNA-dep_ATPase

Graphical View of Domain Structure for InterPro Entry P33991

ProtoNet protein and cluster: P33991

2 Blocks protein families:

IPB001208 MCM family

IPB008047 Mini-chromosome maintenance (MCM) protein 4 signature

UniProtKB/Swiss-Prot: MCM4_HUMAN, P33991

Similarity: Belongs to the MCM family

Similarity: Contains 1 MCM domain

(According to ¹UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or ²DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory,
bound targets from SABiosciences, miRNA Gene Targets from miRTarBase, shRNA from OriGene, Sirion Biotech, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Sirion Biotech, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, Sirion Biotech, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
About This Section

Molecular Function:

UniProtKB/Swiss-Prot Summary: MCM4_HUMAN, P33991

Function: Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential

for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in

the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure

of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the

adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex

helicase activity

Catalytic activity: ATP + H(2)O = ADP + phosphate

Genatlas biochemistry entry for MCM4:

cell division cycle 21(CDC21) homolog,involved in an early step of DNA replication,participating in the cell cycle

control of genome duplication

Enzyme Number (IUBMB): EC 3.6.4.12¹

Gene Ontology (GO): 5/6 molecular function terms (GO ID links to tree view) (see all 6): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0003677	DNA binding	--	--
GO:0003678	DNA helicase activity	--	--
GO:0003697	single-stranded DNA binding	IEA	--
GO:0004003	contributes to ATP-dependent DNA helicase activity	IDA	9305914
GO:0005515	protein binding	IPI	--

MCM4 for ontologies About GeneDecksing

Phenotypes:
8 MGI mutant phenotypes (inferred from 2 alleles) (MGI details for Mcm4):

cellular	digestive/alimentary	embryogenesis	growth/size	hematopoietic system
mortality/aging	normal	tumorigenesis

MCM4 for phenotypes About GeneDecksing

Animal Models:

inGenious Targeting Laboratory - Customizable classic, inducible, and humanized mouse model solutions for MCM4

miRNA Products:		OriGene 3'-UTR Clone (see all 2): MCM4 Browse MicroRNA Expression Plasmids
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		2 QIAGEN miScript miRNA Assays for microRNAs that regulate MCM4:
		hsa-miR-651 hsa-miR-570
		SwitchGear 3'UTR luciferase reporter plasmid: MCM4 3' UTR sequence

Inhib. RNA Products:		Browse for Gene Knock-down Tools from EMD Millipore
		OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for MCM4 (see all 4) OriGene shRNA RFP: MCM4 Browse OriGene siRNA
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Clone Products:		Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore
		OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for MCM4 (see all 3) OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for MCM4 (see all 2) OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
		GenScript: all cDNA clones in your preferred vector (see all 2): MCM4 (NM_005914)
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In Situ Assay
Products:

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for MCM4

(Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to ¹UniProtKB, ²MINT, ³I2D, and/or ⁴STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
About This Section

Unified GeneCards pathways - 5/13 super-pathways (see all 13) About this table

See pathways by source

Super-pathway

contained gene-specific pathways

Ubiquitinated Orc1 is degraded by the proteasome

8/11 pathways (see all 11)
Assembly of the pre-replicative complex	0.77	M/G1 Transition	0.62
Switching of origins to a post-replicative state	0.70	Synthesis of DNA	0.51
Orc1 removal from chromatin	0.70	DNA Replication	0.49
Regulation of DNA replication	0.68	Cell Cycle Checkpoints	0.42

Cell cycle

	Cell cycle	1.00			G1 to S cell cycle control	0.38
	Cell cycle	0.57

Lagging Strand Synthesis

	DNA strand elongation	0.65			Unwinding of DNA	0.00
	DNA replication	0.56

Cell cycle_Start of DNA replication in early S phase

Cell cycle_Start of DNA replication in early S phase

1.00

Cell cycle Start of DNA replication in early S phase

0.98

Activation of ATR in response to replication stress

Activation of ATR in response to replication stress

1.00

G2/M Checkpoints

0.86

Pathway sources
See GeneCards unified pathways
Show all pathways

2 EMD Millipore Pathways for MCM4

	Meiosis
	Cell cycle Start of DNA replication in early S phase

1 Downloadable PowerPoint Slide of QIAGEN Pathway Central Maps for MCM4

Cell Cycle Control of Chromosomal Replication

1 Cell Signaling Technology (CST) Pathway for MCM4

Cell Cycle / Checkpoint Control

1 GeneGo (Thomson Reuters) Pathway for MCM4

Cell cycle Start of DNA replication in early S phase

4 BioSystems Pathways for MCM4

	G1 to S cell cycle control
	Cell cycle
	DNA Replication
	C-MYB transcription factor network

5/21 Reactome Pathways for MCM4 (see all 21)

	Activation of ATR in response to replication stress
	Mitotic G1-G1/S phases
	DNA Replication
	Removal of licensing factors from origins
	Cell Cycle

2 Kegg Pathways (Kegg details for MCM4):

	DNA replication
	Cell cycle

MCM4 for pathways About GeneDecksing

Interactions:

SABiosciences Gene Network Central^TM Interacting Genes and Proteins Network for MCM4

STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

5/282 Interacting proteins for MCM4 (P33991^{1, 2, 3} ENSP00000262105⁴) via UniProtKB, MINT, STRING, and/or I2D (see all 282)

Interactant		Interaction Details
GeneCard	External ID(s)	Interaction Details
SSRP1	Q08945²^,³, ENSP00000278412⁴	MINT-2984401 MINT-6542674 MINT-2984219 MINT-2984419 MINT-2984494 MINT-8173832 MINT-2984477 MINT-8089790 I2D: score=4 STRING: ENSP00000278412
HIST1H4A	P62805³, ENSP00000352980⁴	I2D: score=3 STRING: ENSP00000352980
HIST1H4L	P62805³, ENSP00000348258⁴	I2D: score=3 STRING: ENSP00000348258
HIST1H4B	P62805³	I2D: score=3
HIST1H4C	P62805³	I2D: score=3

About this table

Gene Ontology (GO): 5/10 biological process terms (GO ID links to tree view) (see all 10): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0000075	cell cycle checkpoint	TAS	--
GO:0000082	G1/S transition of mitotic cell cycle	TAS	--
GO:0000084	S phase of mitotic cell cycle	TAS	--
GO:0000216	M/G1 transition of mitotic cell cycle	TAS	--
GO:0000278	mitotic cell cycle	TAS	--

MCM4 for ontologies About GeneDecksing

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
About This Section

MCM4 for compounds About GeneDecksing

Browse Small Molecules at EMD Millipore

Browse drugs & compounds from Enzo Life Sciences

Browse Tocris compounds for MCM4
1 Novoseek chemical compound relationship for MCM4 gene About this table

Compound	-log (P-Val)	Hits	PubMed IDs for Articles with Shared Sentences (# sentences)
hydroxyurea	52.1	4	15037254 (2), 17062569 (1)

Search CenterWatch for drugs/clinical trials and news about MCM4

(Secondary structures according to fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from EMD Millipore,
siRNAs from OriGene, QIAGEN, shRNA from OriGene, Sirion Biotech, microRNA from QIAGEN,
Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
About This Section

REFSEQ mRNAs for MCM4 gene (2 alternative transcripts):

NM_005914.3 NM_182746.2

Unigene Cluster for MCM4:

Minichromosome maintenance complex component 4

Hs.460184 [show with all ESTs]

Unigene Representative Sequence: NM_005914

18 Ensembl transcripts including schematic representations, and UCSC links where relevant:

ENST00000518221 ENST00000523944(uc010lxw.2 uc003xqk.2 uc011ldi.2)

ENST00000262105(uc003xql.2) ENST00000520994 ENST00000520934 ENST00000396826

ENST00000524086 ENST00000519170 ENST00000519138 ENST00000520637 ENST00000518680

ENST00000517709 ENST00000519470 ENST00000523853 ENST00000518382 ENST00000524276

ENST00000521261 ENST00000521151

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		2 QIAGEN miScript miRNA Assays for microRNAs that regulate MCM4:
		hsa-miR-651 hsa-miR-570
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		QIAGEN QuantiFast Probe-based Assays in human, mouse, rat MCM4

Additional cDNA sequence:

AK022899.1 AK296913.1 AK309812.1 AM493756.1 BC031061.1 X74794.1

24/31 DOTS entries (see all 31):

DT.101985052 DT.101985053 DT.101969207 DT.92452784 DT.418116 DT.91831407 DT.101964477 DT.92452786

DT.100793310 DT.100793311 DT.100793307 DT.101978428 DT.408996 DT.120635156 DT.120635241 DT.65286115

DT.97855210 DT.100793312 DT.102831993 DT.102831995 DT.95267949 DT.97855208 DT.100648381 DT.100793314

24/494 AceView cDNA sequences (see all 494):

AI283408 AI936566 BG248645 BQ676347 AU124670 AW236412 BE796484 BQ897289

BM909380 AU120286 BG029854 BC031061 AU131979 AU126357 AI809093 BM844209

BM802681 BM912980 AW575728 AI990498 AK022899 BM761229 BU163628 AI685316

GeneLoc Exon Structure

5/13 Alternative Splicing Database (ASD) splice patterns (SP) for MCM4 (see all 13) About this scheme

ExUns:	1	^	2a	·	2b	·	2c	^	3a	·	3b	^	4a	·	4b	^	5a	·	5b	·	5c	·	5d	^	6	^	7	^	8	^	9a	·	9b	^	10a	·	10b	^	11	^	12	^	13a	·	13b	·	13c	^	14a	·	14b	·
SP1:											-						-																								-
SP2:											-						-																								-
SP3:											-						-		-
SP4:
SP5:

ExUns:	14c	^	15	^	16	^	17	^	18a	·	18b	·	18c	^	19a	·	19b	^	20a	·	20b
SP1:			-						-
SP2:			-						-
SP3:
SP4:
SP5:			-		-				-

ECgene alternative splicing isoforms for MCM4

(RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section

MCM4 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: GTTTGGCTGC

About this image

MCM4 expression in embryonic tissues and stem cells
Expression by the Database of Embryonic development, Stem cell research, and Regenerative medicine About this table

Stem Cell Differentiation: 2 LifeMap Cells

Name

Category

PureStem™ progenitor E44 (Embryonic Progenitor Cell)

PureStem™ progenitor F15 (Embryonic Progenitor Cell)

Expression:

Positive

Negative

Selective marker

Experimental details:

Curated

Microarrays

In-situ hybridization

See MCM4 Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for MCM4

SOURCE GeneReport for Unigene cluster: Hs.460184

SABiosciences Expression via Pathway-Focused PCR Array including MCM4:

Cell Cycle in human mouse rat

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		QIAGEN QuantiFast Probe-based Assays in human, mouse, rat MCM4

In Situ
Assay Products:

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for MCM4

(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD , ⁵MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or ⁶Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
About This Section

This gene was present in the common ancestor of eukaryotes.

Orthologs for MCM4 gene from 10/38 species (see all 38) About this table

Organism	Taxonomic classification	Gene	Description	Human Similarity	Orthology Type	Details
mouse (Mus musculus)	Mammalia	Mcm4^{1 , 5}	minichromosome maintenance deficient 4 homolog (S. more^{1, 5}	88.09(n)¹ 95.01(a)¹		16 (10.09 cM)⁵ 17217¹ NM_008565.3¹ NP_032591.3¹ 15623897⁵
chicken (Gallus gallus)	Aves	MCM4¹	minichromosome maintenance complex component 4	77.3(n) 86.73(a)		426764 XM_424376.3 XP_424376.2
lizard (Anolis carolinensis)	Reptilia	MCM4⁶	--	85(a)	1 ↔ 1	GL343207.1(1525837-1549073)
African clawed frog (Xenopus laevis)	Amphibia	mcm4-A²	minichromosome maintenance deficient 4	77.61(n)		U44049.1
zebrafish (Danio rerio)	Actinopterygii	mcm4²	MCM4 minichromosome maintenance deficient 4, mitotin more	74.85(n)		337598 BC056514.1
fruit fly (Drosophila melanogaster)	Insecta	dpa^{1 , 3}	pre-replicative complex formation and maintenance more³ disc proliferation abnormal¹	61(a)³ 59.73(n)¹ 63.34(a)¹		35679¹ NM_057837.4¹ NP_477185.1¹
worm (Caenorhabditis elegans)	Secernentea	mcm-4^{1 , 3}	Protein MCM-4¹	53(a)³ 54.98(n)¹ 55.82(a)¹		I(2318428-2321811)³ 171793¹ NM_058561.4¹ NP_490962.1¹
baker's yeast (Saccharomyces cerevisiae)	Saccharomycetes	MCM4(YPR019W)⁴ MCM4¹	Essential helicase component of heterohexameric MCM2-7 more⁴ Mcm4p¹	52.31(n)¹ 50.14(a)¹		16(596750-599551)⁴ 856130^{1, 4} NP_015344.1^{1, 4}
thale cress (Arabidopsis thaliana)	eudicotyledons	MCM4¹	minichromosome maintenance protein 4 (cell division more	53.43(n) 46.73(a)		816142 NM_127197.4 NP_179236.3
rice (Oryza sativa)	Liliopsida	--	MCM4 - Putative minichromosome maintenance MCM com...	41(a)	1 ↔ 1	1(20190980-20195959)

ENSEMBL Gene Tree for MCM4 (if available)
TreeFam Gene Tree for MCM4 (if available)

(Paralogs according to ¹HomoloGene,
²Ensembl, and ³SIMAP, Pseudogenes according to ⁴Pseudogene.org Build 68)
About This Section

Paralogs for MCM4 gene

MCM8²

7 SIMAP similar genes for MCM4 using alignment to 10 protein entries: MCM4_HUMAN (see all proteins):

MCM3 FLJ00323 MCM6 MCM7 MCM2 MCM5

HCC5

MCM4 for paralogs About GeneDecksing

(SNPs/Variants according to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section

SNP ID	Valid	Clinical significance	Chr 8 pos	Sequence	#	AA Chg	Type	More	#	Allele freq	Pop	Total sample	More
Genomic Data					Transcription Related Data				Allele Frequencies
				--	--	--		--	--
rs7015408^1,2	C	--	44334276(+)	`tttttG/Tgggac`	2	--	int¹ trp³		0	--	--	--	--
rs11781424^1,2	H	--	44334383(+)	`cctccC/Tgaata`	2	--	int¹		0	--	--	--	--
rs10092257^1,2	A	--	44334972(+)	`cgaggC/Tggggg`	2	--	int¹		0	--	--	--	--
rs8178000^1,2	C,F,H	--	44335468(-)	`CCGCGG/AGTGGG`	2	--	int¹		7		NS EA NA	552
rs17334305^1,2	C,F	--	44336415(+)	`GAGGCC/TCCGCC`	2	--	us2k¹		2		NS NA	182
rs17287548^1,2	C,F,H	--	44336860(+)	`GCTGCC/TGCTTG`	2	--	us2k¹		6		NS NA	736
rs17334312^1,2	C,F	--	44337280(+)	`GCTTTA/GTAACA`	2	--	us2k¹		4		NS NA	184
rs185748348^1,2		--	48870910(+)	`AGAAAA/GCAGCC`	4	--	us2k¹ int¹		0	--	--	--	--
rs79708894^1,2	F	--	48870997(+)	`CTGTAA/GGAGTA`	4	--	us2k¹ int¹		2		WA EA	238
rs143632515^1,2		--	48871093(+)	`TCAAGC/TGAAAG`	4	--	int¹ us2k¹		0	--	--	--	--

HapMap Linkage Disequilibrium report for MCM4 (48872745 - 48890720 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV): 1 variation for MCM4
1 CNV: 3730
Human Gene Mutation Database (HGMD): MCM4

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(in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
About This Section

MCM4 for disorders           About GeneDecksing

OMIM gene information: 602638    OMIM disorders: --

12 diseases for MCM4:    About MalaCards

merkel cell carcinoma chronic lymphocytic leukemia lymphocytic leukemia medulloblastoma

esophageal cancer esophagitis leukemia lung cancer

breast cancer melanoma carcinoma malaria

2 Novoseek disease relationships for MCM4 gene About this table

Disease	-log (P-Val)	Hits	PubMed IDs for Articles with Shared Sentences (# sentences)
metastasis	0	1	17222376 (1)
tumors	0	5	16595783 (1), 16051427 (1), 20398247 (1)

Human Genome Epidemiology (HuGE) Navigator: MCM4 (4 documents)

Export disorders for MCM4 gene to outside databases

(in PubMed. Associations of this gene to articles via ¹Entrez Gene, ²UniProtKB/Swiss-Prot, ³HGNC, ⁴GAD, ⁵PharmGKB, ⁶HMDB, ⁷DrugBank, ⁸UniProtKB/TrEMBL, ⁹ Novoseek, and/or ¹⁰fRNAdb)
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PubMed articles for MCM4 gene, integrated from 9 sources (see all 129):
(articles sorted by number of sources associating them with MCM4)

Utopia: connect your pdf to the dynamic
world of online information

A human homologue of the yeast replication protein Cdc21. Interactions with other Mcm proteins. (PubMed id 7601140)^{1, 2, 3, 9} Musahl C.... Knippers R. (1995)
The promoters for human DNA-PKcs (PRKDC) and MCM4: divergently transcribed genes located at chromosome 8 band q11. (PubMed id 9465298)^{1, 2, 9} Connelly M.A.... Anderson C.W. (1998)
MCM4 and PRKDC, human genes encoding proteins MCM4 and DNA-PKcs, are close neighbours located on chromosome 8q12-->q13. (PubMed id 9284934)^{1, 2, 9} Ladenburger E.M.... Knippers R. (1997)
Identification and characterization of a novel component of the human minichromosome maintenance complex. (PubMed id 17296731)^{1, 2, 9} Sakwe A.M....Frappier L. (2007)
Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. (PubMed id 17081983)^{1, 2} Olsen J.V....Mann M. (2006)
The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)^{1, 2} Gerhard D.S....Malek J. (2004)
A DNA helicase activity is associated with an MCM4, -6, and -7 protein complex. (PubMed id 9305914)^{1, 2} Ishimi Y. (1997)
The P1 family: a new class of nuclear mammalian proteins related to the yeast Mcm replication proteins. (PubMed id 8265339)^{1, 2} Hu B.... Knippers R. (1993)
Site-specific phosphorylation of MCM4 during the cell cycle in mammalian cells. (PubMed id 16519687)^{1, 9} Komamura-Kohno Y....Ishimi Y. (2006)
[Expression and significance of MCM4 in esophageal cancer] (PubMed id 17222376)^{1, 9} Huang X.P....Rong T.H. (2007)

(in PubMed, OMIM, and NCBI Bookshelf)
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		AND	OR
Aliases
Disorders
Free Text

Query String		PubMed OMIM NCBI Bookshelf
	(Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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Entrez Gene: 4173	HGNC: 6947	AceView: MCM4	Ensembl:ENSG00000104738	euGenes: HUgn4173
ECgene: MCM4	Kegg: 4173	H-InvDB: MCM4

(According to HUGE)
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(According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

Name	Description
PharmGKB entry for MCM4	Pharmacogenomics, SNPs, Pathways
ATLAS Chromosomes in Cancer entry for MCM4	Genetics and Cytogenetics in Oncology and Haematology
NIEHS-SNPs	http://egp.gs.washington.edu/data/mcm4/

Intellectual Property for MCM4 gene
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Patent Information for MCM4 gene:
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minichromosome maintenance complex component 4

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minichromosome maintenance complex component 4

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