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MCM3 Gene

protein-coding   GIFtS: 69
GCID: GC06M052128

Minichromosome Maintenance Complex Component 3

(Previous names: minichromosome maintenance deficient (S. cerevisiae) 3,...)
  See MCM3-related diseases
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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Aliases
Minichromosome Maintenance Complex Component 31 2     RLFB2
P1-MCM32 3     Cervical Cancer Proto-Oncogene 52
DNA Polymerase Alpha Holoenzyme-Associated Protein P12 3     DNA Replication Factor MCM32
p1022 3     DNA Replication Licensing Factor MCM32
RLF Subunit Beta2 3     HRlf Beta Subunit2
MCM3 Minichromosome Maintenance Deficient 3 (S. Cerevisiae)1     MCM3 Minichromosome Maintenance Deficient 32
Minichromosome Maintenance Deficient (S. Cerevisiae) 31     Minichromosome Maintenance Deficient 32
HCC52     Replication Licensing Factor, Beta Subunit2
P1.h2     EC 3.6.4.123

External Ids:    HGNC: 69451   Entrez Gene: 41722   Ensembl: ENSG000001121187   OMIM: 6026935   UniProtKB: P252053   

Export aliases for MCM3 gene to outside databases

Previous GC identifers: GC06M052130 GC06M052175 GC06M052236 GC06M051960


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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Entrez Gene summary for MCM3 Gene:
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that
are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM
proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of
replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of
the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein
also interacts with and is acetylated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of
this protein inhibits the initiation of DNA replication and cell cycle progression. Two transcript variants
encoding different isoforms have been found for this gene. (provided by RefSeq, Jul 2012)

GeneCards Summary for MCM3 Gene:
MCM3 (minichromosome maintenance complex component 3) is a protein-coding gene. Diseases associated with MCM3 include cervical cancer, and papillary thyroid carcinoma. GO annotations related to this gene include DNA helicase activity.

UniProtKB/Swiss-Prot: MCM3_HUMAN, P25205
Function: Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase
essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active
ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that
a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site
of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute
differentially to the complex helicase activity. Required for DNA replication and cell proliferation

Gene Wiki entry for MCM3 Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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RefSeq DNA sequence at NCBI GenBank:
NC_000006.11  NC_018917.2  NT_007592.16  
Regulatory elements:
   Regulatory transcription factor binding sites in the MCM3 gene promoter:
         NF-YA   Pax-6   MyoD   NF-YB   CBF-B   CBF-A   ZIC2/Zic2   CP1A   NF-Y   CBF(2)   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidMCM3 promoter sequence
   Search Chromatin IP Primers for MCM3

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat MCM3


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 6p12   Ensembl cytogenetic band:  6p12.2   HGNC cytogenetic band: 6p12

MCM3 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
MCM3 gene location

GeneLoc information about chromosome 6         GeneLoc Exon Structure

GeneLoc location for GC06M052128:  view genomic region     (about GC identifiers)

Start:
52,128,807 bp from pter      End:
52,149,679 bp from pter
Size:
20,873 bases      Orientation:
minus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, Cloud-Clone Corp., and/or eBioscience,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, Cloud-Clone Corp., and/or eBioscience, Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, Cloud-Clone Corp, and/or others.)
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UniProtKB/Swiss-Prot: MCM3_HUMAN, P25205 (See protein sequence)
Recommended Name: DNA replication licensing factor MCM3  
Size: 808 amino acids; 90981 Da
Subunit: Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit
order MCM2-MCM6-MCM4-MCM7-MCM3-MCM5 (By simililarity). Associated with the replication-specific DNA polymerase
alpha. Interacts with MCMBP
Miscellaneous: Early fractionation of eukaryotic MCM proteins yielded a variety of dimeric, trimeric and
tetrameric complexes with unclear biological significance. The MCM2-7 hexamer is the proposed physiological
active complex
Secondary accessions: Q92660 Q9BTR3 Q9NUE7

Explore the universe of human proteins at neXtProt for MCM3: NX_P25205

Explore proteomics data for MCM3 at MOPED

Post-translational modifications: 

  • O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner1
  • Ubiquitination2 at Lys35, Lys266, Lys293, Lys301, Lys351, Lys463, Lys579, Lys635, Lys748
  • Modification sites at PhosphoSitePlus

  • See MCM3 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins (2 alternative transcripts): 
    NP_001257401.1  NP_002379.3  

    ENSEMBL proteins: 
     ENSP00000229854   ENSP00000472940   ENSP00000407651   ENSP00000470057   ENSP00000388647  
    Reactome Protein details: P25205

    MCM3 Human Recombinant Protein Products:

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    OriGene MassSpec for MCM3
    OriGene Custom Protein Services for MCM3
    GenScript Custom Purified and Recombinant Proteins Services for MCM3
    Novus Biologicals MCM3 Protein
    Novus Biologicals MCM3 Lysate
    Browse Sino Biological Recombinant Proteins
    Browse Sino Biological Cell Lysates
    Browse ProSpec Recombinant Proteins
    Cloud-Clone Corp. Proteins for MCM3

     
    Search eBioscience for Proteins for MCM3 

    MCM3 Antibody Products:

    EMD Millipore Mono- and Polyclonal Antibodies for the study of MCM3
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    ThermoFisher Antibody for MCM3
    LSBio Antibodies in human, mouse, rat for MCM3

    MCM3 Assay Products:

    Browse Kits and Assays available from EMD Millipore
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    Browse Enzo Life Sciences for kits & assays
    Cloud-Clone Corp. ELISAs for MCM3
    Cloud-Clone Corp. CLIAs for MCM3
    Search eBioscience for ELISAs for MCM3 


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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    Selected InterPro protein domains (see all 7):
     IPR018525 MCM_CS
     IPR027925 MCM_N
     IPR008046 Mcm3
     IPR012340 NA-bd_OB-fold
     IPR027417 P-loop_NTPase

    Graphical View of Domain Structure for InterPro Entry P25205

    ProtoNet protein and cluster: P25205

    2 Blocks protein domains:
    IPB001208 MCM family
    IPB008046 Mini-chromosome maintenance (MCM) protein 3 family signature


    UniProtKB/Swiss-Prot: MCM3_HUMAN, P25205
    Similarity: Belongs to the MCM family
    Similarity: Contains 1 MCM domain


    Find genes that share domains with MCM3           About GenesLikeMe


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, Vector BioLabs and/or Addgene, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Flow cytometry from eBioscience, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Molecular Function:

         UniProtKB/Swiss-Prot Summary: MCM3_HUMAN, P25205
    Function: Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase
    essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active
    ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that
    a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site
    of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute
    differentially to the complex helicase activity. Required for DNA replication and cell proliferation
    Catalytic activity: ATP + H(2)O = ADP + phosphate

         Genatlas biochemistry entry for MCM3:
    cell division cycle,early S-phase-like-3,participating in the cell cycle control of genome duplication

         Enzyme Number (IUBMB): EC 3.6.4.121

         Gene Ontology (GO): Selected molecular function terms (see all 7):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000166nucleotide binding ----
    GO:0003677DNA binding IEA--
    GO:0003678DNA helicase activity IEA--
    GO:0005515protein binding IPI12364596
    GO:0005524ATP binding IEA--
         
    Find genes that share ontologies with MCM3           About GenesLikeMe


    Phenotypes:
         1 GenomeRNAi human phenotype for MCM3:
     Increased number of cells in m 

         6 MGI mutant phenotypes (inferred from 1 allele(MGI details for Mcm3):
     cellular  embryogenesis  growth/size/body  hematopoietic system  mortality/aging 
     tumorigenesis 

    Find genes that share phenotypes with MCM3           About GenesLikeMe

    Animal Models:
       inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for MCM3
       inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for MCM3

       genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for MCM3
       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for MCM3

    miRNA
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    miRTarBase miRNAs that target MCM3:
    hsa-mir-1 (MIRT023522), hsa-mir-215-5p (MIRT024312), hsa-mir-222-3p (MIRT046726), hsa-mir-92a-3p (MIRT049183), hsa-mir-34a-5p (MIRT025232), hsa-mir-1260b (MIRT052696), hsa-mir-99b-5p (MIRT044198), hsa-mir-192-5p (MIRT026151), hsa-mir-26b-5p (MIRT028827), hsa-mir-193b-3p (MIRT016314), hsa-mir-93-3p (MIRT038726)

    Block miRNA regulation of human, mouse, rat MCM3 using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate MCM3 (see all 21):
    hsa-miR-520e hsa-miR-302d hsa-miR-106a hsa-miR-302e hsa-miR-520a-3p hsa-miR-372 hsa-miR-93 hsa-miR-520d-3p
    SwitchGear 3'UTR luciferase reporter plasmidMCM3 3' UTR sequence
    Inhib. RNA
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    OriGene RNAi products in human, mouse, rat for MCM3
    Predesigned siRNA for gene silencing in human, mouse, rat MCM3

    Gene Editing
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    Clone
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    GenScript: all cDNA clones in your preferred vector: MCM3 (NM_002388)
    Sino Biological Human cDNA Clone for MCM3
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for MCM3
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat MCM3

    Cell Line
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    In Situ Assay
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    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for MCM3

    Flow Cytometry
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    eBioscience FlowRNA Probe Sets ( VA1-12261) for MCM3 


    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Subcellular locations from UniProtKB/Swiss-Prot
    MCM3_HUMAN, P25205: Nucleus
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    nucleus5
    cytoskeleton4

    Gene Ontology (GO): Selected cellular component terms (see all 9):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634nucleus IDA--
    GO:0005654nucleoplasm TAS--
    GO:0005658alpha DNA polymerase:primase complex TAS1549468
    GO:0005730NOT nucleolus IDA--
    GO:0005737cytoplasm ----

    Find genes that share ontologies with MCM3           About GenesLikeMe


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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    SuperPaths for MCM3 About   (see all 11)  
    See pathways by source

    SuperPathSelected contained pathways About (see all per SuperPath)
    1Activation of the pre-replicative complex
    Activation of the pre-replicative complex0.66
    Cell cycle Start of DNA replication in early S phase0.53
    DNA Replication0.66
    2DNA strand elongation
    DNA strand elongation0.63
    Unwinding of DNA0.00
    DNA replication0.63
    3E2F mediated regulation of DNA replication
    Mitotic G1-G1/S phases0.81
    G1 to S cell cycle control0.35
    G1/S Transition0.81
    4Cell cycle
    Cell cycle0.59
    Cell cycle0.59
    5CDK-mediated phosphorylation and removal of Cdc6
    Switching of origins to a post-replicative state0.93
    Removal of licensing factors from origins0.86
    Orc1 removal from chromatin0.93
    M/G1 Transition0.81
    Regulation of DNA replication0.93
    DNA Replication Pre-Initiation0.81
    Synthesis of DNA0.92
    S Phase0.78


    Find genes that share SuperPaths with MCM3           About GenesLikeMe

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways

    1 Downloadable PowerPoint Slide of GeneGlobe Pathway Central Maps for MCM3
        Cell Cycle Control of Chromosomal Replication

    1 Cell Signaling Technology (CST) Pathway for MCM3
        Cell Cycle / Checkpoint Control

    1 GeneGo (Thomson Reuters) Pathway for MCM3
        Cell cycle Start of DNA replication in early S phase

    5 BioSystems Pathways for MCM3
        Cell cycle
    G1 to S cell cycle control
    DNA Replication
    RB in Cancer
    E2F transcription factor network


    Selected Reactome Pathways for MCM3 (see all 7)
        Assembly of the pre-replicative complex
    Unwinding of DNA
    Switching of origins to a post-replicative state
    Activation of ATR in response to replication stress
    Activation of the pre-replicative complex


    2 Kegg Pathways  (Kegg details for MCM3):
        DNA replication
    Cell cycle

        Pathway & Disease-focused RT2 Profiler PCR Array including MCM3: 
              Cell Cycle in human mouse rat

    Interactions:

        GeneGlobe Interaction Network for MCM3

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    Selected Interacting proteins for MCM3 (P252051, 2, 3 ENSP000002298544) via UniProtKB, MINT, STRING, and/or I2D (see all 308)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    HIST1H4BP628051, 3EBI-355153,EBI-302023 I2D: score=3 
    HIST1H4CP628051, 3EBI-355153,EBI-302023 I2D: score=3 
    HIST1H4DP628051, 3EBI-355153,EBI-302023 I2D: score=3 
    HIST1H4EP628051, 3EBI-355153,EBI-302023 I2D: score=3 
    HIST1H4IP628051, 3EBI-355153,EBI-302023 I2D: score=3 
    About this table

    Gene Ontology (GO): Selected biological process terms (see all 7):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000082G1/S transition of mitotic cell cycle TAS--
    GO:0000278mitotic cell cycle TAS--
    GO:0006260DNA replication TAS--
    GO:0006270DNA replication initiation IEA--
    GO:0006271DNA strand elongation involved in DNA replication TAS--

    Find genes that share ontologies with MCM3           About GenesLikeMe



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Browse Tocris compounds for MCM3

    1 Novoseek inferred chemical compound relationship for MCM3 gene    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    estrogen 0 2 14501812 (2)



    Find genes that share compounds with MCM3           About GenesLikeMe



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, and/or Addgene, Primers from OriGene, and/or QIAGEN, Flow cytometry from eBioscience )
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    REFSEQ mRNAs for MCM3 gene (2 alternative transcripts): 
    NM_001270472.1  NM_002388.4  

    Unigene Cluster for MCM3:

    Minichromosome maintenance complex component 3
    Hs.179565  [show with all ESTs]
    Unigene Representative Sequence: NM_002388
    6 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000229854(uc003pan.1 uc011dwu.1) ENST00000596288 ENST00000421471
    ENST00000476448 ENST00000596983 ENST00000419835
    miRNA
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    Selected qRT-PCR Assays for microRNAs that regulate MCM3 (see all 21):
    hsa-miR-520e hsa-miR-302d hsa-miR-106a hsa-miR-302e hsa-miR-520a-3p hsa-miR-372 hsa-miR-93 hsa-miR-520d-3p
    SwitchGear 3'UTR luciferase reporter plasmidMCM3 3' UTR sequence
    Inhib. RNA
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    Predesigned siRNA for gene silencing in human, mouse, rat MCM3
    Clone
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    GenScript: all cDNA clones in your preferred vector: MCM3 (NM_002388)
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    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat MCM3
    Primer
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      QuantiFast Probe-based Assays in human, mouse, rat MCM3
    Flow Cytometry
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    eBioscience FlowRNA Probe Sets ( VA1-12261) for MCM3 

    Additional mRNA sequence: 

    AK222586.1 AK299566.1 AK300754.1 AK301704.1 AK312928.1 AY032603.1 BC001626.1 BC003509.1 
    D38073.1 X62153.2 

    24 DOTS entries:

    DT.95265124  DT.456368  DT.100827031  DT.95265132  DT.99994761  DT.91761440  DT.91761437  DT.92064219 
    DT.121345104  DT.121345133  DT.121345139  DT.99990224  DT.100827037  DT.100827043  DT.102833209  DT.121345126 
    DT.121345128  DT.121345136  DT.91761446  DT.91761450  DT.95205185  DT.95265138  DT.95265140  DT.97861316 

    Selected AceView cDNA sequences (see all 449):

    BM927480 BU857116 BE907579 BU856617 BQ651630 CF529217 BU154249 AU124153 
    BU183082 BQ012986 BU558287 BP378212 BM788699 AA953255 CA407119 BQ063041 
    NM_002388 AW769882 CO399524 BQ061652 CB129538 AL079481 CR592608 BM790143 

    GeneLoc Exon Structure

    Selected Alternative Splicing Database (ASD) splice patterns (SP) for MCM3 (see all 13)    About this scheme

    ExUns: 1a · 1b · 1c ^ 2a · 2b · 2c ^ 3 ^ 4a · 4b ^ 5 ^ 6a · 6b · 6c ^ 7a · 7b ^ 8 ^ 9a · 9b ^ 10 ^ 11a · 11b ^ 12a · 12b · 12c ^ 13 ^ 14a ·
    SP1:                                                        -                                                                 -     -                           
    SP2:                                                                                                                                                            
    SP3:                                                        -                                                                                                   
    SP4:                                                        -     -     -     -     -     -     -     -     -     -     -     -     -                           
    SP5:                                                        -                                                     -                                             

    ExUns: 14b ^ 15a · 15b ^ 16 ^ 17a · 17b ^ 18 ^ 19 ^ 20a · 20b
    SP1:                                -           -               
    SP2:                                -                           
    SP3:                                                            
    SP4:                                                            
    SP5:                                                            


    ECgene alternative splicing isoforms for MCM3

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
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    MCM3 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: CAGGTCAAGA
    MCM3 Expression
    About this image


    MCM3 expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database
     selected tissues (see all 5) fully expand
     
     Trophoblast (Extraembryonic Tissues)
             Trophoblast Cells Trophoblast
     
     Bone (Muscoskeletal System)
             Bone Marrow
     
     Neural Tube (Nervous System)
             Mesencephalic Ventricular Zone
     
     Skeletal Muscle (Muscoskeletal System)
             Muscle Progenitor Cells Thoracic Ventrolateral Dermomyotome Lip
     
     Somite (Muscoskeletal System)
             Muscle Progenitor Cells Thoracic Ventrolateral Dermomyotome Lip
    MCM3 Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    MCM3 Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.179565
        Pathway & Disease-focused RT2 Profiler PCR Array including MCM3: 
              Cell Cycle in human mouse rat

    Primer
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    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for MCM3

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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    This gene was present in the common ancestor of eukaryotes.

    Orthologs for MCM3 gene from Selected species (see all 27)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Mcm31 , 5 minichromosome maintenance deficient 3 (S. cerevisiae)1, 5 88.32(n)1
    93.79(a)1
      1 (6.50 cM)5
    172151  NM_008563.21  NP_032589.11 
     208030145 
    chicken
    (Gallus gallus)
    Aves MCM31 minichromosome maintenance complex component 3 77.1(n)
    81.81(a)
      422043  NM_001006421.1  NP_001006421.1 
    lizard
    (Anolis carolinensis)
    Reptilia MCM36
    minichromosome maintenance complex component 3
    77(a)
    1 ↔ 1
    1(122893736-122921201)
    African clawed frog
    (Xenopus laevis)
    Amphibia mcm3-prov2 MCM3 minichromosome maintenance deficient 3 (S. cerevisiae) 77.79(n)    BC044051.1 
    zebrafish
    (Danio rerio)
    Actinopterygii mcm32 MCM3 minichromosome maintenance deficient 3 (S. cerevisiae) 78.49(n)   192323  BC056718.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta Mcm31 , 3 pre-replicative complex formation and
    maintenance more3
    Minichromosome maintenance 31
    61(a)3
    62(n)1
    64.71(a)1
      4F53
    314491  NM_078493.31  NP_511048.21 
    worm
    (Caenorhabditis elegans)
    Secernentea mcm-33 DNA replication licensing factor MCM3 48(a)   V(15059555-15062189)   --
    baker's yeast
    (Saccharomyces cerevisiae)
    Saccharomycetes MCM3(YEL032W)4
    MCM31
    Protein involved in DNA replication; component of the more4
    MCM31
    54.69(n)1
    54.43(a)1
      5(86937-89852)4
    8566801, 4  NP_010882.11, 4 
    thale cress
    (Arabidopsis thaliana)
    eudicotyledons MCM31 MCM3 54.61(n)
    52.05(a)
      834670  NM_123997.2  NP_199440.1 
    rice
    (Oryza sativa)
    Liliopsida Os.233592 Oryza sativa (japonica cultivar-group) cDNA cloneJ more 72.82(n)    AK063430.1 


    ENSEMBL Gene Tree for MCM3 (if available)
    TreeFam Gene Tree for MCM3 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
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    Paralogs for MCM3 gene
    5 SIMAP similar genes for MCM3 using alignment to 5 protein entries:     MCM3_HUMAN (see all proteins):
    HCC5    MCM4    FLJ00323    MCM2    MCM7

    Find genes that share paralogs with MCM3           About GenesLikeMe


    1 Pseudogenes.org Pseudogene for MCM3
    PGOHUM00000234917


    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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    Selected SNPs for MCM3 (see all 676)    About this table                                 

    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 6 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs669961341,2
    C--51979186(+) AAAAA-/A/    
       AAAAA
    TAGGT
    2 -- int11NA 2
    rs1464856321,2
    --52128335(+) GCTGTA/GATTGT 2 -- ds50010--------
    rs349427431,2
    C--52128383(+) GTCTCAAA/-AAAAA 2 -- ds50011Minor allele frequency- -:0.50NA 2
    rs1410374861,2
    --52128460(+) TGAGGA/TGTCTG 2 -- ds50010--------
    rs1148980681,2
    C,F--52128468(+) CTGACG/ATATCG 2 -- ds50011Minor allele frequency- A:0.02WA 118
    rs17377481,2
    C,O,H--52128473(-) ATACCT/CGATAC 2 -- ds500115Minor allele frequency- C:0.00NA WA CSA EA 516
    rs17810101,2
    C,H--52128495(+) GAAGGG/ATAAGT 2 -- ds500116Minor allele frequency- A:0.00NS EA NA WA CSA 795
    rs1456821151,2
    --52128520(+) AAAGCA/GCCAGG 2 -- ds50010--------
    rs172402871,2
    C,F--52128553(-) GAGTAA/GAAGTT 2 -- ds50011Minor allele frequency- G:0.01NS 150
    rs172402801,2
    C,F--52128630(-) GCGTTT/CATCAG 2 -- ds50012Minor allele frequency- C:0.02NS NA 274

    HapMap Linkage Disequilibrium report for MCM3 (52128807 - 52149679 bp)

    Structural Variations
         Database of Genomic Variants (DGV) 1 variation for MCM3:    About this table    
    Variant IDTypeSubtypePubMed ID
    nsv830662CNV Gain17160897

    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing MCM3
    DNA2.0 Custom Variant and Variant Library Synthesis for MCM3

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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    OMIM gene information: 602693    OMIM disorders: --

    2 diseases for MCM3:    
    About MalaCards
    cervical cancer    papillary thyroid carcinoma


    Find genes that share disorders with MCM3           About GenesLikeMe

    6 Novoseek inferred disease relationships for MCM3 gene    About this table

    Disease   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    anaplastic astrocytoma 44.2 1 15671553 (1)
    breast cancer 0 1 15623617 (1)
    tumors 0 8 15623617 (2), 19818763 (2), 15671553 (1), 19389522 (1)
    carcinoma 0 2 15623617 (1), 10495426 (1)
    melanoma 0 1 15623617 (1)
    cancer 0 9 15623617 (5), 12631269 (2), 11745678 (1)

    Genetic Association Database (GAD): MCM3
    Human Genome Epidemiology (HuGE) Navigator: MCM3 (2 documents)

    Export disorders for MCM3 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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    PubMed articles for MCM3 gene, integrated from 10 sources (see all 160):
    (articles sorted by number of sources associating them with MCM3)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Properties of the nuclear P1 protein, a mammalian homologue of the yeast Mcm3 replication protein. (PubMed id 1549468)1, 2, 3 Thoemmes P.... Knippers R. (Nucleic Acids Res. 1992)
    2. Identification and characterization of a novel component of the human minichromosome maintenance complex. (PubMed id 17296731)1, 2, 9 Sakwe A.M.... Frappier L. (Mol. Cell. Biol. 2007)
    3. Minichromosome maintenance proteins are direct targets of the ATM and ATR checkpoint kinases. (PubMed id 15210935)1, 2, 9 Cortez D.... Elledge S.J. (Proc. Natl. Acad. Sci. U.S.A. 2004)
    4. Centrosome-related genes, genetic variation, and risk of breast cancer. (PubMed id 20508983)1, 4 Olson J.E....Couch F.J. (Breast Cancer Res. Treat. 2011)
    5. A probability-based approach for high-throughput protein phosphorylation analysis and site localization. (PubMed id 16964243)1, 2 Beausoleil S.A.... Gygi S.P. (Nat. Biotechnol. 2006)
    6. Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. (PubMed id 17081983)1, 2 Olsen J.V....Mann M. (Cell 2006)
    7. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S.... Malek J. (Genome Res. 2004)
    8. The DNA sequence and analysis of human chromosome 6. (PubMed id 14574404)1, 2 Mungall A.J.... Beck S. (Nature 2003)
    9. Identification of the yeast MCM3-related protein as a component of Xenopus DNA replication licensing factor. (PubMed id 7758114)1, 2 Kubota Y.... Nojima H. (Cell 1995)
    10. The P1 family: a new class of nuclear mammalian proteins related to the yeast Mcm replication proteins. (PubMed id 8265339)1, 2 Hu B.... Knippers R. (Nucleic Acids Res. 1993)

    (in PubMed, OMIM, and NCBI Bookshelf)
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     ANDOR
    Aliases
    Disorders
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Entrez Gene: 4172 HGNC: 6945 AceView: MCM3 Ensembl:ENSG00000112118 euGenes: HUgn4172
    ECgene: MCM3 Kegg: 4172 H-InvDB: MCM3

    (According to HUGE)
    About This Section

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      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
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    NameDescription
    PharmGKB entry for MCM3 Pharmacogenomics, SNPs, Pathways
    ATLAS Chromosomes in Cancer entry for MCM3 Genetics and Cytogenetics in Oncology and Haematology
    NIEHS-SNPshttp://egp.gs.washington.edu/data/mcm3/

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
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    Patent Information for MCM3 gene:
    Search GeneIP for patents involving MCM3

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, eBioscience, and/or others, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Addgene, Cell lines from GenScript, and ESI BIO, Flow cytometery from eBioscience, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
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