Set Analyses:
Advanced Search

Advanced Search

 
Search By
Section (entire)
for


 



MCM2 Gene

protein-coding   GIFtS: 71
GCID: GC03P127317

Minichromosome Maintenance Complex Component 2

(Previous names: minichromosome maintenance deficient (S. cerevisiae) 2 (mitotin),...)
(Previous symbols: CCNL1, CDCL1)
  See MCM2-related disease
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
About This Section

TryGeneCards Plus

Aliases
Minichromosome Maintenance Complex Component 21 2     MITOTIN2
CDCL11 2 3 5     cdc192
CCNL11 2 3     Cell Devision Cycle-Like 12
Minichromosome Maintenance Protein 2 Homolog2 3     Cyclin-Like 12
Nuclear Protein BM282 3     DNA Replication Licensing Factor MCM22
BM282 3     MCM2 Minichromosome Maintenance Deficient 2, Mitotin2
MCM2 Minichromosome Maintenance Deficient 2, Mitotin (S. Cerevisiae)1     Minichromosome Maintenance Deficient 2 (Mitotin)2
Minichromosome Maintenance Deficient (S. Cerevisiae) 2 (Mitotin)1     EC 3.6.4.123
mitotin1     KIAA00303
D3S31942     

External Ids:    HGNC: 69441   Entrez Gene: 41712   Ensembl: ENSG000000731117   OMIM: 1169455   UniProtKB: P497363   

Export aliases for MCM2 gene to outside databases

Previous GC identifers: GC03P124148 GC03P127476 GC03P128598 GC03P128638 GC03P128799 GC03P124697


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

TryGeneCards Plus

Entrez Gene summary for MCM2 Gene:
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that
are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM
proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of
replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex
with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is
phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7. Multiple alternatively spliced transcript
variants have been found, but the full-length nature of some variants has not been defined. (provided by RefSeq,
Oct 2012)

GeneCards Summary for MCM2 Gene:
MCM2 (minichromosome maintenance complex component 2) is a protein-coding gene. Diseases associated with MCM2 include cervix endometriosis. GO annotations related to this gene include DNA replication origin binding and histone binding. An important paralog of this gene is MCM9.

UniProtKB/Swiss-Prot: MCM2_HUMAN, P49736
Function: Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase
essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active
ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that
a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site
of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute
differentially to the complex helicase activity. Required for the entry in S phase and for cell division

Gene Wiki entry for MCM2 Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
About This Section

TryGeneCards Plus
RefSeq DNA sequence at NCBI GenBank:
NC_000003.11  NC_018914.2  NT_005612.17  
Regulatory elements:
   Regulatory transcription factor binding sites in the MCM2 gene promoter:
         GR   p53   ATF-2   CUTL1   N-Myc   HOXA5   FOXJ2 (long isoform)   c-Jun   FOXJ2   GR-alpha   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidMCM2 promoter sequence
   Search Chromatin IP Primers for MCM2

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat MCM2


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 3q21   Ensembl cytogenetic band:  3q21.3   HGNC cytogenetic band: 3q21

MCM2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
MCM2 gene location

GeneLoc information about chromosome 3         GeneLoc Exon Structure

GeneLoc location for GC03P127317:  view genomic region     (about GC identifiers)

Start:
127,317,066 bp from pter      End:
127,341,279 bp from pter
Size:
24,214 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, Cloud-Clone Corp., and/or eBioscience,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, Cloud-Clone Corp., and/or eBioscience, Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, Cloud-Clone Corp, and/or others.)
About This Section

TryGeneCards Plus

UniProtKB/Swiss-Prot: MCM2_HUMAN, P49736 (See protein sequence)
Recommended Name: DNA replication licensing factor MCM2  
Size: 904 amino acids; 101896 Da
Subunit: Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit
order MCM2-MCM6-MCM4-MCM7-MCM3-MCM5 (By simililarity). Interacts with DBF4 (By similarity). Interacts with KAT7.
May interact with MCM10
Miscellaneous: Early fractionation of eukaryotic MCM proteins yielded a variety of dimeric, trimeric and
tetrameric complexes with unclear biological significance. Specifically a MCM467 subcomplex is shown to have in
vitro helicase activity which is inhibited by the MCM2 subunit. The MCM2-7 hexamer is the proposed physiological
active complex
Sequence caution: Sequence=BAA04642.1; Type=Erroneous initiation; Note=Translation N-terminally shortened;
Sequence=BAA12177.1; Type=Erroneous initiation; Note=Translation N-terminally extended; Sequence=CAA47749.1;
Type=Erroneous initiation; Note=Translation N-terminally shortened; Sequence=CAA47749.1; Type=Frameshift;
Positions=115, 124, 127, 129, 154, 158, 773, 811;
Secondary accessions: Q14577 Q15023 Q8N2V1 Q969W7 Q96AE1 Q9BRM7

Explore the universe of human proteins at neXtProt for MCM2: NX_P49736

Explore proteomics data for MCM2 at MOPED

Post-translational modifications: 

  • Phosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents.
    Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by
    the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation
    of DNA replication1
  • Ubiquitination2 at Lys178, Lys469, Lys476, Lys492, Lys613, Lys722
  • Modification sites at PhosphoSitePlus

  • See MCM2 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins: NP_004517.2  
    ENSEMBL proteins: 
     ENSP00000265056   ENSP00000420007   ENSP00000419802   ENSP00000418930   ENSP00000417800  
     ENSP00000420528  
    Reactome Protein details: P49736

    MCM2 Human Recombinant Protein Products:

    Browse Purified and Recombinant Proteins at EMD Millipore
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Browse OriGene full length recombinant human proteins expressed in human HEK293 cells
    OriGene Protein Over-expression Lysate for MCM2
    OriGene Custom MassSpec
    OriGene Custom Protein Services for MCM2
    GenScript Custom Purified and Recombinant Proteins Services for MCM2
    Novus Biologicals MCM2 Protein
    Novus Biologicals MCM2 Lysate
    Browse Sino Biological Recombinant Proteins
    Browse Sino Biological Cell Lysates
    Browse ProSpec Recombinant Proteins
    Cloud-Clone Corp. Proteins for MCM2

     
    Search eBioscience for Proteins for MCM2 

    MCM2 Antibody Products:

    EMD Millipore Mono- and Polyclonal Antibodies for the study of MCM2
    R&D Systems Antibodies for MCM2
    Cell Signaling Technology (CST) Antibodies for MCM2 
    OriGene Antibodies for MCM2
    OriGene Custom Antibody Services for MCM2
    Novus Biologicals MCM2 Antibodies
    Abcam antibodies for MCM2
    Cloud-Clone Corp. Antibodies for MCM2
    ThermoFisher Antibody for MCM2
    LSBio Antibodies in human, mouse, rat for MCM2

    MCM2 Assay Products:

    Browse Kits and Assays available from EMD Millipore
    OriGene Custom Assay Services for MCM2
    Browse R&D Systems for biochemical assays
    GenScript Custom Assay Services for MCM2
    Browse Enzo Life Sciences for kits & assays
    Cloud-Clone Corp. ELISAs for MCM2
    Cloud-Clone Corp. CLIAs for MCM2
    Search eBioscience for ELISAs for MCM2 


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    TryGeneCards Plus
    Selected InterPro protein domains (see all 6):
     IPR008045 MCM2
     IPR018525 MCM_CS
     IPR027925 MCM_N
     IPR012340 NA-bd_OB-fold
     IPR027417 P-loop_NTPase

    Graphical View of Domain Structure for InterPro Entry P49736

    ProtoNet protein and cluster: P49736

    2 Blocks protein domains:
    IPB001208 MCM family
    IPB008045 Mini-chromosome maintenance (MCM) protein 2 signature


    UniProtKB/Swiss-Prot: MCM2_HUMAN, P49736
    Similarity: Belongs to the MCM family
    Similarity: Contains 1 MCM domain


    Find genes that share domains with MCM2           About GenesLikeMe


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, Vector BioLabs and/or Addgene, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Flow cytometry from eBioscience, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
    About This Section

    TryGeneCards Plus

    Molecular Function:

         UniProtKB/Swiss-Prot Summary: MCM2_HUMAN, P49736
    Function: Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase
    essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active
    ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that
    a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site
    of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute
    differentially to the complex helicase activity. Required for the entry in S phase and for cell division
    Catalytic activity: ATP + H(2)O = ADP + phosphate

         Genatlas biochemistry entry for MCM2:
    cell division cycle,early S-phase-like 1,involved in an early step of DNA replication,participating in the cell
    cycle control of genome duplication

         Enzyme Number (IUBMB): EC 3.6.4.121

         Gene Ontology (GO): Selected molecular function terms (see all 9):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003677DNA binding TAS8175912
    GO:0003678DNA helicase activity IEA--
    GO:0003688DNA replication origin binding IEA--
    GO:0005515protein binding IPI11095689
    GO:0005524ATP binding IEA--
         
    Find genes that share ontologies with MCM2           About GenesLikeMe


    Phenotypes:
         1 GenomeRNAi human phenotype for MCM2:
     Increased cell death in breast 

         Selected MGI mutant phenotypes (inferred from 3 alleles(MGI details for Mcm2) (see all 17):
     adipose tissue  behavior/neurological  cellular  digestive/alimentary  embryogenesis 
     endocrine/exocrine gland  growth/size/body  hematopoietic system  immune system  integument 
     mortality/aging  muscle  nervous system  normal  pigmentation 

    Find genes that share phenotypes with MCM2           About GenesLikeMe

    Animal Models:
       inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for MCM2
       inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for MCM2

       genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for MCM2
       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for MCM2

    miRNA
    Products:
        
    miRTarBase miRNAs that target MCM2:
    hsa-mir-31-5p (MIRT007327), hsa-mir-423-3p (MIRT042538), hsa-mir-615-3p (MIRT039755), hsa-mir-34a-5p (MIRT025400), hsa-mir-1 (MIRT023837), hsa-mir-501-3p (MIRT037990), hsa-mir-500a-5p (MIRT038004), hsa-mir-1296-5p (MIRT000473), hsa-mir-1226-3p (MIRT036508)

    Block miRNA regulation of human, mouse, rat MCM2 using miScript Target Protectors
    1 qRT-PCR Assays for microRNA that regulate MCM2:
    hsa-miR-4272
    SwitchGear 3'UTR luciferase reporter plasmidMCM2 3' UTR sequence
    Inhib. RNA
    Products:
        
    OriGene RNAi products in human, mouse, rat for MCM2
    Predesigned siRNA for gene silencing in human, mouse, rat MCM2

    Gene Editing
    Products:
    DNA2.0 Custom Protein Engineering Service for MCM2

    Clone
    Products:
         
    OriGene clones in human, mouse for MCM2 (see all 6)
    OriGene ORF clones in mouse, rat for MCM2
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector: MCM2 (NM_004526)
    Browse Sino Biological Human cDNA Clones
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for MCM2
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat MCM2

    Cell Line
    Products:
         
    GenScript Custom overexpressing Cell Line Services for MCM2
    Browse ESI BIO Cell Lines and PureStem Progenitors for MCM2 
    In Situ Assay
    Products:
       

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for MCM2

    Flow Cytometry
    Products:
       

     
    eBioscience FlowRNA Probe Sets ( VA1-12143) for MCM2 


    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
    About This Section

    TryGeneCards Plus

    Subcellular locations from UniProtKB/Swiss-Prot
    MCM2_HUMAN, P49736: Nucleus
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    nucleus5

    Gene Ontology (GO): Selected cellular component terms (see all 8):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000785chromatin IDA16899510
    GO:0005634nucleus IDA--
    GO:0005654nucleoplasm TAS--
    GO:0005664nuclear origin of replication recognition complex IEA--
    GO:0005730NOT nucleolus IDA--

    Find genes that share ontologies with MCM2           About GenesLikeMe


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
    About This Section

    TryGeneCards Plus

    SuperPaths for MCM2 About   (see all 11)  
    See pathways by source

    SuperPathSelected contained pathways About (see all per SuperPath)
    1Activation of the pre-replicative complex
    Activation of the pre-replicative complex0.66
    Cell cycle Start of DNA replication in early S phase0.53
    DNA Replication0.66
    2DNA strand elongation
    DNA strand elongation0.63
    Unwinding of DNA0.00
    DNA replication0.63
    3E2F mediated regulation of DNA replication
    Mitotic G1-G1/S phases0.81
    G1 to S cell cycle control0.35
    G1/S Transition0.81
    4Cell cycle
    Cell cycle0.59
    Cell cycle0.59
    5CDK-mediated phosphorylation and removal of Cdc6
    Switching of origins to a post-replicative state0.93
    Removal of licensing factors from origins0.86
    Orc1 removal from chromatin0.93
    M/G1 Transition0.81
    Regulation of DNA replication0.93
    DNA Replication Pre-Initiation0.81
    Synthesis of DNA0.92
    S Phase0.78


    Find genes that share SuperPaths with MCM2           About GenesLikeMe

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways

    1 Downloadable PowerPoint Slide of GeneGlobe Pathway Central Maps for MCM2
        Cell Cycle Control of Chromosomal Replication

    1 Cell Signaling Technology (CST) Pathway for MCM2
        Cell Cycle / Checkpoint Control

    2 GeneGo (Thomson Reuters) Pathways for MCM2
        Cell cycle Transition and termination of DNA replication
    Cell cycle Start of DNA replication in early S phase

    3 BioSystems Pathways for MCM2
        Cell cycle
    G1 to S cell cycle control
    DNA Replication

    Selected Reactome Pathways for MCM2 (see all 7)
        Assembly of the pre-replicative complex
    Unwinding of DNA
    Switching of origins to a post-replicative state
    Activation of ATR in response to replication stress
    Activation of the pre-replicative complex


    2 Kegg Pathways  (Kegg details for MCM2):
        DNA replication
    Cell cycle

        Pathway & Disease-focused RT2 Profiler PCR Arrays including MCM2: 
              Cell Cycle in human mouse rat
              Cancer PathwayFinder in human mouse rat

    Interactions:

        GeneGlobe Interaction Network for MCM2

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    Selected Interacting proteins for MCM2 (P497361, 2, 3 ENSP000002650564) via UniProtKB, MINT, STRING, and/or I2D (see all 310)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    HIST1H4BP628051, 3EBI-374819,EBI-302023 I2D: score=5 
    HIST1H4CP628051, 3EBI-374819,EBI-302023 I2D: score=5 
    HIST1H4DP628051, 3EBI-374819,EBI-302023 I2D: score=5 
    HIST1H4EP628051, 3EBI-374819,EBI-302023 I2D: score=5 
    HIST1H4IP628051, 3EBI-374819,EBI-302023 I2D: score=5 
    About this table

    Gene Ontology (GO): Selected biological process terms (see all 11):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000082G1/S transition of mitotic cell cycle TAS--
    GO:0000278mitotic cell cycle TAS--
    GO:0006260DNA replication TAS--
    GO:0006268DNA unwinding involved in DNA replication IEA--
    GO:0006270DNA replication initiation IMP16899510

    Find genes that share ontologies with MCM2           About GenesLikeMe



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
    About This Section

    TryGeneCards Plus
    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Browse Tocris compounds for MCM2

    5 Novoseek inferred chemical compound relationships for MCM2 gene    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    paraffin 13.4 3 19293805 (1), 15735170 (1), 11839717 (1)
    threonine 0 1 17046832 (1)
    alanine 0 1 17046832 (1)
    estrogen 0 1 14501812 (1)
    serine 0 2 17046832 (1), 16864800 (1)



    Find genes that share compounds with MCM2           About GenesLikeMe



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, and/or Addgene, Primers from OriGene, and/or QIAGEN, Flow cytometry from eBioscience )
    About This Section

    TryGeneCards Plus

    REFSEQ mRNAs for MCM2 gene: 
    NM_004526.3  

    Unigene Cluster for MCM2:

    Minichromosome maintenance complex component 2
    Hs.477481  [show with all ESTs]
    Unigene Representative Sequence: NR_073375
    9 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000265056(uc003ejp.3 uc011bkm.2 uc010hsl.3 uc011bkn.2)
    ENST00000474964 ENST00000480910 ENST00000472731 ENST00000477668 ENST00000468659
    ENST00000491422 ENST00000473785 ENST00000468414
    miRNA
    Products:
         
    Block miRNA regulation of human, mouse, rat MCM2 using miScript Target Protectors
    1 qRT-PCR Assays for microRNA that regulate MCM2:
    hsa-miR-4272
    SwitchGear 3'UTR luciferase reporter plasmidMCM2 3' UTR sequence
    Inhib. RNA
    Products:
         
    OriGene RNAi products in human, mouse, rat for MCM2
    Predesigned siRNA for gene silencing in human, mouse, rat MCM2
    Clone
    Products:
         
    OriGene clones in human, mouse for MCM2 (see all 6)
    OriGene ORF clones in mouse, rat for MCM2
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector: MCM2 (NM_004526)
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for MCM2
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat MCM2
    Primer
    Products:
        
    OriGene qPCR primer pairs and template standards for MCM2
    OriGene qSTAR qPCR primer pairs in human, mouse for MCM2
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat MCM2
      QuantiTect SYBR Green Assays in human, mouse, rat MCM2
      QuantiFast Probe-based Assays in human, mouse, rat MCM2
    Flow Cytometry
    Products:
       

     
    eBioscience FlowRNA Probe Sets ( VA1-12143) for MCM2 

    Additional mRNA sequence: 

    AK128291.1 AK299934.1 BC000300.2 BC006165.1 BC007670.2 BC007938.2 BC014272.2 BC017258.2 
    BC017490.1 BC030131.2 BT009734.1 D21063.1 D83987.1 NR_073375.1 X67334.1 

    9 DOTS entries:

    DT.119111  DT.100783511  DT.101960187  DT.100783507  DT.91725754  DT.97855950  DT.120861149  DT.95303402 
    DT.95303406 

    Selected AceView cDNA sequences (see all 362):

    CR605781 BE302906 BC014272 BC007670 BQ961276 BQ957649 AI275174 BC000300 
    BG826399 BU528650 BM756916 BU556833 AW467315 CF780814 BM795266 BM910405 
    BM793682 AA626883 CF121359 BU191613 BQ056535 BQ435444 BU196553 BM821302 

    GeneLoc Exon Structure

    5 Alternative Splicing Database (ASD) splice patterns (SP) for MCM2    About this scheme

    ExUns: 1a · 1b ^ 2a · 2b ^ 3a · 3b ^ 4a · 4b ^ 5 ^ 6 ^ 7 ^ 8 ^ 9 ^ 10 ^ 11 ^ 12 ^ 13 ^ 14a · 14b ^ 15 ^ 16 ^ 17 ^ 18a · 18b
    SP1:              -     -                                                                                   -                                       
    SP2:              -     -           -     -     -                                                           -                                       
    SP3:                                                                                                                                                
    SP4:                                                                                                                                                
    SP5:                                                                                                                                                


    ECgene alternative splicing isoforms for MCM2

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    TryGeneCards Plus

    MCM2 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: CGGATTATCC
    MCM2 Expression
    About this image


    MCM2 expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database
     selected tissues (see all 4) fully expand
     
     Neural Tube (Nervous System)    fully expand to see all 4 entries
             Metencephalon
     
     Trophoblast (Extraembryonic Tissues)
             Trophoblast Cells Trophoblast
     
     Bone (Muscoskeletal System)
             Bone Marrow
     
     Brain (Nervous System)
             Medulla Oblongata
    MCM2 Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    MCM2 Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.477481
        Pathway & Disease-focused RT2 Profiler PCR Arrays including MCM2: 
              Cell Cycle in human mouse rat
              Cancer PathwayFinder in human mouse rat

    Primer
    Products:
    OriGene qPCR primer pairs and template standards for MCM2
    OriGene qSTAR qPCR primer pairs in human, mouse for MCM2
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat MCM2
    QuantiTect SYBR Green Assays in human, mouse, rat MCM2
    QuantiFast Probe-based Assays in human, mouse, rat MCM2
    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for MCM2

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    TryGeneCards Plus

    This gene was present in the common ancestor of eukaryotes.

    Orthologs for MCM2 gene from Selected species (see all 28)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Mcm21 , 5 minichromosome maintenance deficient 2 mitotin (S. cerevisiae) less1, 5 87.32(n)1
    95.58(a)1
      6 (39.64 cM)5
    172161  NM_008564.21  NP_032590.21 
     888834755 
    chicken
    (Gallus gallus)
    Aves MCM21 minichromosome maintenance complex component 2 81.11(n)
    90.07(a)
      416027  NM_001006139.1  NP_001006139.1 
    lizard
    (Anolis carolinensis)
    Reptilia MCM26
    minichromosome maintenance complex component 2
    89(a)
    1 ↔ 1
    2(166209259-166230612)
    African clawed frog
    (Xenopus laevis)
    Amphibia mcm2-prov2 DNA replication licensing factor MCM2 78.03(n)    U44047.1 
    zebrafish
    (Danio rerio)
    Actinopterygii mcm22 MCM2 minichromosome maintenance deficient 2, mitotin (S. cerevisiae) less 79.94(n)   192338  BC048026.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta Mcm21 , 3 pre-replicative complex formation and
    maintenance DNA binding less3
    Minichromosome maintenance 21
    59(a)3
    62.91(n)1
    65.48(a)1
      3 84F63
    409731  NM_057773.31  NP_477121.11 
    worm
    (Caenorhabditis elegans)
    Secernentea mcm-21 , 3 MCM2/3/5 family3
    mcm-21
    53(a)3
    57.36(n)1
    59.22(a)1
      II(12001573-12006508)3
    1748411  NM_001027245.51  NP_001022416.11 
    baker's yeast
    (Saccharomyces cerevisiae)
    Saccharomycetes MCM2(YBL023C)4
    MCM21
    Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex less4
    MCM21
    51.96(n)1
    54.13(a)1
      2(177526-174920)4
    8522581, 4  NP_009530.11, 4 
    thale cress
    (Arabidopsis thaliana)
    eudicotyledons MCM21 MCM2 54.2(n)
    52.52(a)
      841056  NM_103572.4  NP_175112.2 
    rice
    (Oryza sativa)
    Liliopsida Os.129742 Oryza sativa (japonica cultivar-group) cDNA cloneJ023133N01, full insert sequence less 73.39(n)    AK121422.1 


    ENSEMBL Gene Tree for MCM2 (if available)
    TreeFam Gene Tree for MCM2 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section

    TryGeneCards Plus
    Paralogs for MCM2 gene
    MCM92  
    6 SIMAP similar genes for MCM2 using alignment to 7 protein entries:     MCM2_HUMAN (see all proteins):
    FLJ00323    MCM9    MCM6    MCM3    MCM7    MCM4

    Find genes that share paralogs with MCM2           About GenesLikeMe



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
    About This Section

    TryGeneCards Plus

    Selected SNPs for MCM2 (see all 688)    About this table                                 

    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 3 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1115042451,2
    C,F--127315229(+) CCCAGG/AATAAT 2 -- us2k12Minor allele frequency- A:0.04NA 122
    rs1841613871,2
    --127315309(+) CATCCA/GAGGTG 2 -- us2k10--------
    rs175383771,2
    C,F--127315495(+) GGATAT/CGTCCC 2 -- us2k11Minor allele frequency- C:0.01NS 180
    rs1478915851,2
    --127315760(+) GGAGAC/TCAGCC 2 -- us2k10--------
    rs27202621,2
    C,F,A,H--127315788(-) agagaG/Tgggat 2 -- us2k111Minor allele frequency- T:0.44NA NS WA CSA 318
    rs1873230651,2
    --127315828(+) ATGGCA/GTGCAT 2 -- us2k10--------
    rs1416924571,2
    C--127315896(+) GGAGGC/TTGCAG 2 -- us2k10--------
    rs1115898371,2
    C--127315957(+) GTCTC-/AAAAAA 2 -- us2k11Minor allele frequency- A:0.50CSA 2
    rs1470332821,2
    --127316038(+) GGGTCA/CCAGGC 2 -- us2k10--------
    rs174968461,2
    C,F--127316284(+) ATACAG/CAATAG 2 -- us2k11Minor allele frequency- C:0.01NS 180

    HapMap Linkage Disequilibrium report for MCM2 (127317066 - 127341279 bp)

    Structural Variations
         Database of Genomic Variants (DGV) 4 variations for MCM2:    About this table    
    Variant IDTypeSubtypePubMed ID
    esv2653998CNV Deletion19546169
    esv2102273CNV Deletion18987734
    esv2555442CNV Deletion19546169
    dgv5249n71CNV Loss21882294

    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing MCM2
    DNA2.0 Custom Variant and Variant Library Synthesis for MCM2

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    TryGeneCards Plus
    OMIM gene information: 116945    OMIM disorders: --

    1 disease for MCM2:    
    About MalaCards
    cervix endometriosis

    2 diseases from the University of Copenhagen DISEASES database for MCM2:
    Cervix endometriosis     Carcinoma

    Find genes that share disorders with MCM2           About GenesLikeMe

    Selected Novoseek inferred disease relationships for MCM2 gene (see all 19)    About this table

    Disease   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    dysplasia 49.2 27 12855631 (6), 11872961 (3), 19116018 (2), 16832409 (2) (see all 9)
    laryngeal squamous cell carcinoma 41 4 16832409 (1), 12966424 (1)
    carcinoma squamous cell 39.3 2 12949987 (1), 16832409 (1)
    adenocarcinoma 39.2 22 18803052 (5), 17940502 (4), 19077563 (2), 19188825 (1) (see all 6)
    adenoma follicular 37.6 1 16722928 (1)
    bladder carcinoma 33.6 3 12565771 (2)
    barrett esophagus 32.3 2 12855631 (1)
    tumors 29.2 36 11948116 (4), 12949987 (3), 18465232 (3), 18774496 (3) (see all 11)
    carcinoma 21.5 10 19293805 (3), 18373176 (3), 17013685 (1), 12966424 (1) (see all 5)
    cancer 19.6 9 18286467 (2), 16545117 (2), 16112007 (1), 18373176 (1) (see all 6)

    Genetic Association Database (GAD): MCM2
    Human Genome Epidemiology (HuGE) Navigator: MCM2 (3 documents)

    Export disorders for MCM2 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    TryGeneCards Plus

    PubMed articles for MCM2 gene, integrated from 10 sources (see all 263):
    (articles sorted by number of sources associating them with MCM2)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. The human gene for nuclear protein BM28 (CDCL1), a new member of the early S-phase family of proteins, maps to chromosome band 3q21. (PubMed id 8258304)1, 2, 3, 9 Mincheva A.... Lichter P. (Cytogenet. Cell Genet. 1994)
    2. Essential role of phosphorylation of MCM2 by Cdc7/Dbf4 in the initiation of DNA replication in mammalian cells. (PubMed id 16899510)1, 2, 9 Tsuji T.... Jiang W. (Mol. Biol. Cell 2006)
    3. Nuclear matrix protein mitotin messenger RNA is expressed at constant levels during the cell cycle. (PubMed id 1710453)1, 3, 9 Todorov I.T....Bibor-Hardy V. (Biochem. Biophys. Res. Commun. 1991)
    4. The human homolog of Saccharomyces cerevisiae Mcm10 interacts with replication factors and dissociates from nuclease-resistant nuclear structures in G(2) phase. (PubMed id 11095689)1, 2, 9 Izumi M.... Hanaoka F. (Nucleic Acids Res. 2000)
    5. Centrosome-related genes, genetic variation, and risk of breast cancer. (PubMed id 20508983)1, 4 Olson J.E....Couch F.J. (Breast Cancer Res. Treat. 2011)
    6. Evaluation of candidate stromal epithelial cross-talk genes identifies association between risk of serous ovarian cancer and TERT, a cancer susceptibility &quot;hot-spot&quot;. (PubMed id 20628624)1, 4 Johnatty S.E.... . (PLoS Genet. 2010)
    7. Identification and characterization of a novel component of the human minichromosome maintenance complex. (PubMed id 17296731)1, 2 Sakwe A.M.... Frappier L. (Mol. Cell. Biol. 2007)
    8. ING tumor suppressor proteins are critical regulators of chromatin acetylation required for genome expression and perpetuation. (PubMed id 16387653)1, 2 Doyon Y.... Cote J. (Mol. Cell 2006)
    9. A probability-based approach for high-throughput protein phosphorylation analysis and site localization. (PubMed id 16964243)1, 2 Beausoleil S.A.... Gygi S.P. (Nat. Biotechnol. 2006)
    10. Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. (PubMed id 17081983)1, 2 Olsen J.V....Mann M. (Cell 2006)

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

    TryGeneCards Plus
     ANDOR
    Aliases
    Disorders
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section

    TryGeneCards Plus
    Entrez Gene: 4171 HGNC: 6944 AceView: MCM2 Ensembl:ENSG00000073111 euGenes: HUgn4171
    ECgene: MCM2 Kegg: 4171 H-InvDB: MCM2

    (According to HUGE)
    About This Section

    TryGeneCards Plus
    HUGE: KIAA0030

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
    About This Section

    TryGeneCards Plus
    NameDescription
    PharmGKB entry for MCM2 Pharmacogenomics, SNPs, Pathways
    ATLAS Chromosomes in Cancer entry for MCM2 Genetics and Cytogenetics in Oncology and Haematology
    NIEHS-SNPshttp://egp.gs.washington.edu/data/mcm2/

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section

    TryGeneCards Plus
    Patent Information for MCM2 gene:
    Search GeneIP for patents involving MCM2

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, eBioscience, and/or others, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Addgene, Cell lines from GenScript, and ESI BIO, Flow cytometery from eBioscience, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
    Advanced Cell Diagnostics, Animal models from inGenious Targeting Laboratory, genOway)
    About This Section

    TryGeneCards Plus

     Browse Small Molecules at EMD Millipore
     Browse Purified and Recombinant Proteins at EMD Millipore
     Browse Kits and Assays available from EMD Millipore
     EMD Millipore Mono- and Polyclonal Antibodies for the study of MCM2
     
     EMD Millipore genomic analysis products

      
     Antibodies for MCM2 (MCM2)   Browse Cell Culture Products  
     Browse ELISAs   Browse Flow Cytometry Kits  
     Browse Primer Pairs   Browse Enzyme Activity Assays/Reagents  
     Browse ELISpot/FluoroSpot Kits/Development Modules   Browse TFB/Immunoprecipitation Assays  
     Browse Apoptosis Detection Kits/Reagents   Browse Ubiquitin Proteasome Pathway (UPP) Assay Kits/Reagents  
     Browse DNA Damage/Repair Kits/Reagents   Browse Luminex Assays  
     Browse Cell Selection/Detection Kits/Reagents   Browse Secondary Antibodies/Controls/Staining Reagents  
     Browse Recombinant/Natural Proteins   Browse Stem Cell Products  
     Browse cDNA Clones   Browse Proteome Profiler Antibody Arrays  
     OriGene Antibodies for MCM2   OriGene RNAi products in human, mouse, rat for MCM2  
     OriGene qPCR primer pairs and template standards for MCM2   OriGene Protein Over-expression Lysate for MCM2  
     OriGene Custom Mass Spec   OriGene clones in human, mouse for MCM2  
     OriGene qSTAR qPCR primer pairs in human, mouse for MCM2   Browse OriGene full length recombinant human proteins expressed in human HEK293 cells  
     OriGene ORF clones in mouse, rat for MCM2   OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling  
     OriGene Custom Antibody Services for MCM2   OriGene Custom Protein Services for MCM2  

     
     
     Block miRNA regulation of human, mouse, rat MCM2 using miScript Target Protectors SeqTarget long-range PCR primers for resequencing MCM2
     DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat MCM2 Predesigned siRNA for gene silencing in human, mouse, rat MCM2
     QuantiFast Probe-based Assays in human, mouse, rat MCM2 QuantiTect SYBR Green Assays in human, mouse, rat MCM2
     PCR Arrays including human, mouse, rat MCM2 Search Chromatin IP Primers for MCM2
     Pre-validated RT2 qPCR Primer Assay in human, mouse, rat MCM2  GeneGlobe Interaction Network for MCM2
     Regulatory tfbs in MCM2 promoter
     GenScript Custom Purified and Recombinant Proteins Services for MCM2 GenScript cDNA clones with any tag delivered in your preferred vector for MCM2
     GenScript Custom Assay Services for MCM2 GenScript Custom overexpressing Cell Line Services for MCM2
     CloneReady with Over 120,000 Genes  Gene Synthesis: Any Gene in Any Vector
     Vector-based siRNA and miRNA, Ready for Transfection Gene Mutant Library, Variants up to 10^11
     Plasmid Preparation Custom Peptide Services
     Antibodies & Assays for MCM2 

     Search Tocris compounds for MCM2
     Browse Sino Biological Proteins
     Browse Sino Biological Cell Lysates
     Browse Sino Biological cDNA Clones
     4000+ Proteins
     Search Sino Biological for antibodies, proteins & pathways
     Protein Production Services
     Transfection Reagents
     Protein A/G/L resins
     Isotyping reagents
     Search www.enzolifesciences.com for proteins, assays, substrates, inhibitors & antibodies

     Novus Tissue Slides
     MCM2 antibodies
     MCM2 proteins
     MCM2 lysates
     Antibodies for MCM2
     See all of Abcam's Antibodies, Kits and Proteins for MCM2
     Custom Antibody / Protein Production Service
     Bulk Purchasing
     Advantages of Rabbit Monoclonal antibodies
     Abcam protocols and scientific support
     Browse ProSpec Recombinant Proteins
     Proteins for MCM2
     Antibodies for MCM2
     ELISAs for MCM2
     CLIAs for MCM2



     Browse ESI BIO Cell Lines and PureStem Progenitors for MCM2
     Gene Synthesis
     Protein Engineering
     Variant Library Synthesis
     Codon Optimization
     Protein Production and Purification
     Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for MCM2
     SwitchGear 3'UTR luciferase reporter plasmids for MCM2
     SwitchGear Promoter luciferase reporter plasmids for MCM2
     ThermoFisher Antibody for MCM2
     Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat MCM2
     inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for MCM2
     inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for MCM2
     LSBio Antibodies in human, mouse, rat for MCM2
    Customized transgenic rodents for:
     Humanization
     Biomarker expression
     Off-target effect monitoring
     Translational medicine
     Tissue-specific gene expresssion
     Time-controlled gene expresssion
     Browse compounds at ApexBio
     Search Addgene for plasmids for MCM2
      Search eBioscience for proteins for MCM2
      Search eBioscience for elisas for MCM2
      eBioscience FlowRNA Probe Sets
           
    GeneCards Homepage - Last full update: 7 May 2014 - Incrementals: 9 May 2014 , 2 Jun 2014 , 26 Jun 2014 , 30 Jun 2014 , 21 Aug 2014 , 8 Sep 2014

    View Random Gene

    Category
    (GIFtS: )
    GIFtS Group
    The GeneCards human gene database gene index: 1 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 


    Developed at the Crown Human Genome Center, Department of Molecular Genetics, the Weizmann Institute of Science

    Hot genes      Disease genes      MCM2 gene at Home site.
    Version: 3.12.224 13 Oct 2014
    hostname: 356980-web2.xennexinc.com index build: 128 solr: 1.4