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Aliases for MCF2 Gene

Aliases for MCF2 Gene

  • MCF.2 Cell Line Derived Transforming Sequence 2 3
  • DBL 3 4 6
  • Oncogene MCF2 (Oncogene DBL) 2 3
  • Proto-Oncogene MCF-2 3 4
  • Proto-Oncogene DBL 3
  • ARHGEF21 3

External Ids for MCF2 Gene

Previous GeneCards Identifiers for MCF2 Gene

  • GC0XM133248
  • GC0XM135521
  • GC0XM136608
  • GC0XM137369
  • GC0XM138389
  • GC0XM138491
  • GC0XM138663
  • GC0XM127931

Summaries for MCF2 Gene

Entrez Gene Summary for MCF2 Gene

  • The oncogenic protein encoded by this gene is a guanine nucleotide exchange factor (GEF) that exerts control over some members of the Rho family of small GTPases. Several transcript variants encoding different isoforms have been found for this gene. These isoforms exhibit different expression patterns and varying levels of GEF activity.[provided by RefSeq, Jan 2010]

GeneCards Summary for MCF2 Gene

MCF2 (MCF.2 Cell Line Derived Transforming Sequence) is a Protein Coding gene. Diseases associated with MCF2 include aarskog-scott syndrome and scott syndrome. Among its related pathways are Signaling by FGFR and Signaling by GPCR. GO annotations related to this gene include phospholipid binding and Rho guanyl-nucleotide exchange factor activity. An important paralog of this gene is ARHGEF40.

UniProtKB/Swiss-Prot for MCF2 Gene

  • Guanine nucleotide exchange factor (GEF) that modulates the Rho family of GTPases. Promotes the conversion of some member of the Rho family GTPase from the GDP-bound to the GTP-bound form. Isoform 1 exhibits no activity toward RHOA, RAC1 or CDC42. Isoform 2 exhibits decreased GEF activity toward CDC42. Isoform 3 exhibits a weak but significant activity toward RAC1 and CDC42. Isoform 4 exhibits significant activity toward RHOA and CDC42. The truncated DBL oncogene is active toward RHOA, RAC1 and CDC42

Gene Wiki entry for MCF2 Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for MCF2 Gene

Genomics for MCF2 Gene

Regulatory Elements for MCF2 Gene

Epigenetics Products

  • DNA Methylation CpG Assay Predesigned for Pyrosequencing in human,mouse,rat

Genomic Location for MCF2 Gene

Start:
139,581,770 bp from pter
End:
139,708,227 bp from pter
Size:
126,458 bases
Orientation:
Minus strand

Genomic View for MCF2 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for MCF2 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for MCF2 Gene

Proteins for MCF2 Gene

  • Protein details for MCF2 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P10911-MCF2_HUMAN
    Recommended name:
    Proto-oncogene DBL
    Protein Accession:
    P10911
    Secondary Accessions:
    • B7Z3Y5
    • B7Z869
    • B7ZAV1
    • E9PH77
    • F5H091
    • P14919
    • Q5JYJ2
    • Q5JYJ3
    • Q5JYJ4
    • Q8IUF3
    • Q8IUF4
    • Q9UJB3

    Protein attributes for MCF2 Gene

    Size:
    925 amino acids
    Molecular mass:
    107673 Da
    Quaternary structure:
    • Interacts with an array of inositol phospholipids such as phosphatidylinositol 3-phosphate (PI3P), phosphatidylinositol 4-phosphate (PI4P) and phosphatidylinositol 5-phosphate (PI5P). May interact with CCPG1.
    SequenceCaution:
    • Sequence=AAA52172.1; Type=Erroneous initiation; Evidence={ECO:0000305};

    Alternative splice isoforms for MCF2 Gene

neXtProt entry for MCF2 Gene

Proteomics data for MCF2 Gene at MOPED

Post-translational modifications for MCF2 Gene

  • Phosphorylation by TNK2 enhances guanine nucleotide exchange factor (GEF) activity toward Rho family proteins.
  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

No data available for DME Specific Peptides for MCF2 Gene

Domains for MCF2 Gene

Gene Families for MCF2 Gene

HGNC:
  • ARHGEF :Rho guanine nucleotide exchange factors

Graphical View of Domain Structure for InterPro Entry

P10911

UniProtKB/Swiss-Prot:

MCF2_HUMAN :
  • P10911
Domain:
  • The CRAL-TRIO domain is involved in interaction with inositol phospholipids
  • The DH domain is essential for transforming activity and directly catalyzes GDP-GTP exchange activity. It may interact with CCPG1
  • Contains 1 CRAL-TRIO domain.
  • Contains 1 DH (DBL-homology) domain.
  • Contains 1 PH domain.
Family:
  • Belongs to the MCF2 family.
Similarity:
  • Contains 1 spectrin repeat.
genes like me logo Genes that share domains with MCF2: view

Function for MCF2 Gene

Molecular function for MCF2 Gene

GENATLAS Biochemistry: transforming sequence,MCF.2 cell line derived,highly expressed in brain,specifically activating CDC42 and not RAC or RHOA
UniProtKB/Swiss-Prot Function: Guanine nucleotide exchange factor (GEF) that modulates the Rho family of GTPases. Promotes the conversion of some member of the Rho family GTPase from the GDP-bound to the GTP-bound form. Isoform 1 exhibits no activity toward RHOA, RAC1 or CDC42. Isoform 2 exhibits decreased GEF activity toward CDC42. Isoform 3 exhibits a weak but significant activity toward RAC1 and CDC42. Isoform 4 exhibits significant activity toward RHOA and CDC42. The truncated DBL oncogene is active toward RHOA, RAC1 and CDC42

Gene Ontology (GO) - Molecular Function for MCF2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005089 Rho guanyl-nucleotide exchange factor activity IEA --
GO:0005515 protein binding IPI 18470881
genes like me logo Genes that share ontologies with MCF2: view
genes like me logo Genes that share phenotypes with MCF2: view

Animal Models for MCF2 Gene

MGI Knock Outs for MCF2:

Animal Model Products

CRISPR Products

No data available for Enzyme Numbers (IUBMB) , miRNA , Transcription Factor Targeting and HOMER Transcription for MCF2 Gene

Localization for MCF2 Gene

Subcellular locations from UniProtKB/Swiss-Prot for MCF2 Gene

Cytoplasm.
Isoform 1: Membrane.
Isoform 3: Membrane. Note=Colocalizes with CDC42 to plasma membrane.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for MCF2 Gene COMPARTMENTS Subcellular localization image for MCF2 gene
Compartment Confidence
cytoskeleton 4
cytosol 4
nucleus 2
plasma membrane 2
mitochondrion 1

Gene Ontology (GO) - Cellular Components for MCF2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005829 cytosol TAS --
GO:0005856 cytoskeleton TAS 3056717
GO:0016020 membrane IEA --
GO:0070062 extracellular exosome IDA 23376485
genes like me logo Genes that share ontologies with MCF2: view

Pathways for MCF2 Gene

genes like me logo Genes that share pathways with MCF2: view

Gene Ontology (GO) - Biological Process for MCF2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0007264 small GTPase mediated signal transduction TAS --
GO:0016358 dendrite development IEA --
GO:0035023 regulation of Rho protein signal transduction --
GO:0035556 intracellular signal transduction --
GO:0043065 positive regulation of apoptotic process TAS --
genes like me logo Genes that share ontologies with MCF2: view

Compounds for MCF2 Gene

(3) HMDB Compounds for MCF2 Gene

Compound Synonyms Cas Number PubMed IDs
Guanosine diphosphate
  • 5'-GDP
146-91-8
Guanosine monophosphate
  • 5'-GMP
85-32-5
Guanosine triphosphate
  • 5'-GTP
86-01-1

(12) Novoseek inferred chemical compound relationships for MCF2 Gene

Compound -log(P) Hits PubMed IDs
gdp 81.3 18
gtp 73.6 13
guanine 39.4 1
tyrosine 29.7 8
arginine 16.6 5
genes like me logo Genes that share compounds with MCF2: view

Transcripts for MCF2 Gene

Unigene Clusters for MCF2 Gene

MCF.2 cell line derived transforming sequence:
Representative Sequences:

CRISPR Products

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for MCF2

Primer Products

  • OriGene qSTAR qPCR primer pairs in human,mouse,rat for MCF2

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for MCF2 Gene

No ASD Table

Relevant External Links for MCF2 Gene

GeneLoc Exon Structure for
MCF2
ECgene alternative splicing isoforms for
MCF2

Expression for MCF2 Gene

mRNA expression in normal human tissues for MCF2 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for MCF2 Gene

This gene is overexpressed in Adrenal Gland (6.9), Testis (4.8), Brain - Hypothalamus (4.4), and Brain - Frontal Cortex (BA9) (4.1).

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, and MOPED for MCF2 Gene

SOURCE GeneReport for Unigene cluster for MCF2 Gene Hs.387262

mRNA Expression by UniProt/SwissProt for MCF2 Gene

P10911-MCF2_HUMAN
Tissue specificity: Isoform 1 is expressed only in brain. Isoform 3 is expressed in heart, kidney, spleen, liver and testis. Isoform 4 is expressed in brain, heart, kidney, testis, placenta, stomach and peripheral blood. The protein is detectable in brain, heart, kidney, intestine, muscle, lung and testis.
genes like me logo Genes that share expressions with MCF2: view

In Situ Assay Products

Orthologs for MCF2 Gene

This gene was present in the common ancestor of animals.

Orthologs for MCF2 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia MCF2 35
  • 96.42 (n)
  • 95.63 (a)
cow
(Bos Taurus)
Mammalia MCF2 35
  • 88.62 (n)
  • 86.55 (a)
MCF2 36
  • 86 (a)
OneToOne
dog
(Canis familiaris)
Mammalia MCF2 35
  • 88.38 (n)
  • 86.95 (a)
MCF2 36
  • 86 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Mcf2 35
  • 81.16 (n)
  • 76.05 (a)
Mcf2 16
Mcf2 36
  • 74 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia MCF2 36
  • 57 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia MCF2 36
  • 60 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Mcf2 35
  • 80.03 (n)
  • 73.82 (a)
chicken
(Gallus gallus)
Aves MCF2 35
  • 68.73 (n)
  • 64.86 (a)
MCF2 36
  • 58 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia MCF2 36
  • 57 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia mcf2 35
  • 65.01 (n)
  • 58.77 (a)
zebrafish
(Danio rerio)
Actinopterygii mcf2a 35
  • 58.32 (n)
  • 54.05 (a)
mcf2a 36
  • 47 (a)
OneToMany
mcf2b 36
  • 47 (a)
OneToMany
fruit fly
(Drosophila melanogaster)
Insecta CG30440 36
  • 23 (a)
OneToMany
CG30440 37
  • 39 (a)
worm
(Caenorhabditis elegans)
Secernentea cgef-1 36
  • 21 (a)
OneToMany
sea squirt
(Ciona savignyi)
Ascidiacea -- 36
  • 33 (a)
OneToMany
Species with no ortholog for MCF2:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for MCF2 Gene

ENSEMBL:
Gene Tree for MCF2 (if available)
TreeFam:
Gene Tree for MCF2 (if available)

Paralogs for MCF2 Gene

Selected SIMAP similar genes for MCF2 Gene using alignment to 4 proteins:

genes like me logo Genes that share paralogs with MCF2: view

Variants for MCF2 Gene

Sequence variations from dbSNP and Humsavar for MCF2 Gene

SNP ID Clin Chr 0X pos Sequence Context AA Info Type MAF
rs761143 -- 139,635,664(+) AAAAA(A/T)ATATA intron-variant
rs761144 -- 139,635,764(+) CAAAG(G/T)TTACA intron-variant
rs761145 -- 139,635,821(+) TCACC(C/T)AGGTA intron-variant
rs761146 -- 139,635,833(+) TAAGC(C/T)CAGTA intron-variant
rs764159 -- 139,611,980(+) AAGAA(A/G)aagtg intron-variant

Structural Variations from Database of Genomic Variants (DGV) for MCF2 Gene

Variant ID Type Subtype PubMed ID
nsv528242 CNV Gain 19592680
nsv523170 CNV Gain 19592680
esv268694 CNV Insertion 20981092

Relevant External Links for MCF2 Gene

HapMap Linkage Disequilibrium report
MCF2
Human Gene Mutation Database (HGMD)
MCF2
Locus Specific Mutation Databases (LSDB)
MCF2

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for MCF2 Gene

Disorders for MCF2 Gene

MalaCards: The human disease database

MalaCards: The human disease database. (2) Diseases for MCF2 Gene including...

Search for MCF2 Gene in MalaCards »

UniProtKB/Swiss-Prot

MCF2_HUMAN
  • Note=MCF2 and DBL represent two activated versions of the same proto-oncogene.

(2) University of Copenhagen DISEASES for MCF2 Gene

(5) Novoseek inferred disease relationships for MCF2 Gene

Disease -log(P) Hits PubMed IDs
lymphoma b-cell 11.5 2
dysplasia 6.74 3
leukemia 2.92 2
tumors 0 1
lymphoma 0 1

Relevant External Links for MCF2

Genetic Association Database (GAD)
MCF2
Tumor Gene Database (TGDB):
MCF2
genes like me logo Genes that share disorders with MCF2: view

Publications for MCF2 Gene

  1. Localization of the 5' end of the MCF2 oncogene to human chromosome 15q15----q23. (PMID: 1611909) Galland F. … Birnbaum D. (Cytogenet. Cell Genet. 1992) 2 3 23
  2. Activation of the guanine nucleotide exchange factor Dbl following ACK1-dependent tyrosine phosphorylation. (PMID: 10652228) Kato J. … Satoh T. (Biochem. Biophys. Res. Commun. 2000) 3 4 23
  3. Alternative splicing variants of the human DBL (MCF-2) proto- oncogene. (PMID: 12445822) Komai K. … Shiozawa S. (Biochem. Biophys. Res. Commun. 2002) 3 4 23
  4. Activation of a mcf.2 oncogene by deletion of amino-terminal coding sequences. (PMID: 2577874) Noguchi T. … Birnbaum D. (Oncogene 1988) 2 3 4
  5. Molecular cloning and characterization of the human dbl proto- oncogene: evidence that its overexpression is sufficient to transform NIH/3T3 cells. (PMID: 3056717) Ron D. … Eva A. (EMBO J. 1988) 3 4

Products for MCF2 Gene

Sources for MCF2 Gene

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