Set Analyses:
Advanced Search

Advanced Search

 
Search By
Section (entire)
for


 



MCF2 Gene

protein-coding   GIFtS: 64
GCID: GC0XM138663

MCF.2 Cell Line Derived Transforming Sequence

  See MCF2-related diseases
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
About This Section

TryGeneCards Plus

Aliases
MCF.2 Cell Line Derived Transforming Sequence1 2
DBL2 3 5
Oncogene MCF2 (Oncogene DBL)1 2
Proto-Oncogene MCF-22 3
ARHGEF212
Proto-Oncogene DBL2

External Ids:    HGNC: 69401   Entrez Gene: 41682   Ensembl: ENSG000001019777   OMIM: 3110305   UniProtKB: P109113   

Export aliases for MCF2 gene to outside databases

Previous GC identifers: GC0XM133248 GC0XM135521 GC0XM136608 GC0XM137369 GC0XM138389 GC0XM138491 GC0XM127931


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

TryGeneCards Plus

Entrez Gene summary for MCF2 Gene:
The oncogenic protein encoded by this gene is a guanine nucleotide exchange factor (GEF) that exerts control over
some members of the Rho family of small GTPases. Several transcript variants encoding different isoforms have
been found for this gene. These isoforms exhibit different expression patterns and varying levels of GEF
activity.(provided by RefSeq, Jan 2010)

GeneCards Summary for MCF2 Gene:
MCF2 (MCF.2 cell line derived transforming sequence) is a protein-coding gene. Diseases associated with MCF2 include aarskog-scott syndrome, and scott syndrome. GO annotations related to this gene include phospholipid binding and Rho guanyl-nucleotide exchange factor activity. An important paralog of this gene is KALRN.

UniProtKB/Swiss-Prot: MCF2_HUMAN, P10911
Function: Guanine nucleotide exchange factor (GEF) that modulates the Rho family of GTPases. Promotes the
conversion of some member of the Rho family GTPase from the GDP-bound to the GTP-bound form. Isoform 1 exhibits
no activity toward RHOA, RAC1 or CDC42. Isoform 2 exhibits decreased GEF activity toward CDC42. Isoform 3
exhibits a weak but significant activity toward RAC1 and CDC42. Isoform 4 exhibits significant activity toward
RHOA and CDC42. The truncated DBL oncogene is active toward RHOA, RAC1 and CDC42

Gene Wiki entry for MCF2 Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
About This Section

TryGeneCards Plus
RefSeq DNA sequence at NCBI GenBank:
NC_000023.10  NT_011786.17  NC_018934.2  
Regulatory elements:
   Regulatory transcription factor binding sites in the MCF2 gene promoter:
         AhR   AML1a   GATA-3   Arnt   GATA-1   E47   HFH-1   Hand1   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidMCF2 promoter sequence
   Search Chromatin IP Primers for MCF2

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat MCF2


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: Xq27   Ensembl cytogenetic band:  Xq27.1   HGNC cytogenetic band: Xq26.3-q27.1

MCF2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
MCF2 gene location

GeneLoc information about chromosome X         GeneLoc Exon Structure

GeneLoc location for GC0XM138663:  view genomic region     (about GC identifiers)

Start:
138,663,929 bp from pter      End:
138,790,386 bp from pter
Size:
126,458 bases      Orientation:
minus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, Cloud-Clone Corp., and/or eBioscience,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, Cloud-Clone Corp., and/or eBioscience, Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, Cloud-Clone Corp, and/or others.)
About This Section

TryGeneCards Plus

UniProtKB/Swiss-Prot: MCF2_HUMAN, P10911 (See protein sequence)
Recommended Name: Proto-oncogene DBL  
Size: 925 amino acids; 107673 Da
Subunit: Interacts with an array of inositol phospholipids such as phosphatidylinositol 3-phosphate (PI3P),
phosphatidylinositol 4-phosphate (PI4P) and phosphatidylinositol 5-phosphate (PI5P). May interact with CCPG1
Sequence caution: Sequence=AAA52172.1; Type=Erroneous initiation;
Secondary accessions: B7Z3Y5 B7Z869 B7ZAV1 E9PH77 F5H091 P14919 Q5JYJ2 Q5JYJ3 Q5JYJ4 Q8IUF3
Q8IUF4 Q9UJB3
Alternative splicing: 6 isoforms:  P10911-1   P10911-2   P10911-3   P10911-4   P10911-5   P10911-6   (No experimental confirmation available)

Explore the universe of human proteins at neXtProt for MCF2: NX_P10911

Explore proteomics data for MCF2 at MOPED

Post-translational modifications: 

  • Phosphorylation by TNK2 enhances guanine nucleotide exchange factor (GEF) activity toward Rho family proteins1
  • Modification sites at neXtProt
  • Modification sites at PhosphoSitePlus

  • See MCF2 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins (6 alternative transcripts): 
    NP_001093325.1  NP_001165347.1  NP_001165348.1  NP_001165349.1  NP_001165350.1  NP_005360.3  

    ENSEMBL proteins: 
     ENSP00000427745   ENSP00000403271   ENSP00000359608   ENSP00000405848   ENSP00000430276  
     ENSP00000359605   ENSP00000342204   ENSP00000438155   ENSP00000359610   ENSP00000397055  
    Reactome Protein details: P10911

    MCF2 Human Recombinant Protein Products:

    Browse Purified and Recombinant Proteins at EMD Millipore
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Browse OriGene full length recombinant human proteins expressed in human HEK293 cells
    OriGene Protein Over-expression Lysate for MCF2
    OriGene Custom MassSpec
    OriGene Custom Protein Services for MCF2
    GenScript Custom Purified and Recombinant Proteins Services for MCF2
    Browse Sino Biological Recombinant Proteins
    Browse Sino Biological Cell Lysates
    Browse ProSpec Recombinant Proteins
    Cloud-Clone Corp. Proteins for MCF2

     
    Search eBioscience for Proteins for MCF2 

    MCF2 Antibody Products:

    Browse EMD Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Browse R&D Systems for Antibodies
    Cell Signaling Technology (CST) Antibodies for MCF2 
    Browse OriGene Antibodies
    OriGene Custom Antibody Services for MCF2
    Novus Biologicals MCF2 Antibody
    Abcam antibodies for MCF2
    Cloud-Clone Corp. Antibodies for MCF2
    ThermoFisher Antibodies for MCF2
    LSBio Antibodies in human, mouse, rat for MCF2

    MCF2 Assay Products:

    Browse Kits and Assays available from EMD Millipore
    OriGene Custom Assay Services for MCF2
    Browse R&D Systems for biochemical assays
    GenScript Custom Assay Services for MCF2
    Browse Enzo Life Sciences for kits & assays
    Cloud-Clone Corp. ELISAs for MCF2
    Cloud-Clone Corp. CLIAs for MCF2
    Search eBioscience for ELISAs for MCF2 


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    TryGeneCards Plus
    HGNC Gene Families:
    ARHGEF: Rho guanine nucleotide exchange factors

    Selected InterPro protein domains (see all 6):
     IPR000219 DH-domain
     IPR001849 Pleckstrin_homology
     IPR001251 CRAL-TRIO_dom
     IPR011993 PH_like_dom
     IPR018159 Spectrin/alpha-actinin

    Graphical View of Domain Structure for InterPro Entry P10911

    ProtoNet protein and cluster: P10911

    4 Blocks protein domains:
    IPB000219 DH domain
    IPB001849 Pleckstrin-like
    IPB002017 Spectrin repeat
    IPB003957 CCAAT-binding transcription factor subunit A signature


    UniProtKB/Swiss-Prot: MCF2_HUMAN, P10911
    Domain: The CRAL-TRIO domain is involved in interaction with inositol phospholipids
    Domain: The DH domain is essential for transforming activity and directly catalyzes GDP-GTP exchange activity. It
    may interact with CCPG1
    Similarity: Belongs to the MCF2 family
    Similarity: Contains 1 CRAL-TRIO domain
    Similarity: Contains 1 DH (DBL-homology) domain
    Similarity: Contains 1 PH domain
    Similarity: Contains 1 spectrin repeat


    Find genes that share domains with MCF2           About GenesLikeMe


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, Vector BioLabs and/or Addgene, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Flow cytometry from eBioscience, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
    About This Section

    TryGeneCards Plus

    Molecular Function:

         UniProtKB/Swiss-Prot Summary: MCF2_HUMAN, P10911
    Function: Guanine nucleotide exchange factor (GEF) that modulates the Rho family of GTPases. Promotes the
    conversion of some member of the Rho family GTPase from the GDP-bound to the GTP-bound form. Isoform 1 exhibits
    no activity toward RHOA, RAC1 or CDC42. Isoform 2 exhibits decreased GEF activity toward CDC42. Isoform 3
    exhibits a weak but significant activity toward RAC1 and CDC42. Isoform 4 exhibits significant activity toward
    RHOA and CDC42. The truncated DBL oncogene is active toward RHOA, RAC1 and CDC42

         Genatlas biochemistry entry for MCF2:
    transforming sequence,MCF.2 cell line derived,highly expressed in brain,specifically activating CDC42 and not RAC
    or RHOA

         Gene Ontology (GO): 2 molecular function terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005089Rho guanyl-nucleotide exchange factor activity IEA--
    GO:0005515protein binding IPI18470881
         
    Find genes that share ontologies with MCF2           About GenesLikeMe


    Phenotypes:
         3 MGI mutant phenotypes (inferred from 1 allele(MGI details for Mcf2):
     behavior/neurological  nervous system  reproductive system 

    Find genes that share phenotypes with MCF2           About GenesLikeMe

    Animal Models:
         MGI mouse knock-out Mcf2tm1Ehi for MCF2

       inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for MCF2
       inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for MCF2

       genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for MCF2
       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for MCF2

    miRNA
    Products:
        
    Block miRNA regulation of human, mouse, rat MCF2 using miScript Target Protectors
    6 qRT-PCR Assays for microRNAs that regulate MCF2:
    hsa-miR-124 hsa-miR-143 hsa-miR-506 hsa-miR-218 hsa-miR-636 hsa-miR-570
    SwitchGear 3'UTR luciferase reporter plasmidMCF2 3' UTR sequence
    Inhib. RNA
    Products:
        
    OriGene RNAi products in human, mouse, rat for MCF2
    Predesigned siRNA for gene silencing in human, mouse, rat MCF2

    Gene Editing
    Products:
    DNA2.0 Custom Protein Engineering Service for MCF2

    Clone
    Products:
         
    OriGene clones in human, mouse for MCF2 (see all 28)
    OriGene ORF clones in mouse, rat for MCF2
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector (see all 6): MCF2 (NM_005369)
    Browse Sino Biological Human cDNA Clones
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for MCF2
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat MCF2

    Cell Line
    Products:
         
    GenScript Custom overexpressing Cell Line Services for MCF2
    Browse ESI BIO Cell Lines and PureStem Progenitors for MCF2 
    In Situ Assay
    Products:
       

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for MCF2


    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
    About This Section

    TryGeneCards Plus

    Subcellular locations from UniProtKB/Swiss-Prot
    MCF2_HUMAN, P10911: Cytoplasm
    MCF2_HUMAN, P10911: Isoform 1: Membrane
    MCF2_HUMAN, P10911: Isoform 3: Membrane. Note=Colocalizes with CDC42 to plasma membrane
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    cytoskeleton4
    cytosol4
    nucleus2
    plasma membrane2
    mitochondrion1

    Gene Ontology (GO): 3 cellular component terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005829cytosol TAS--
    GO:0005856cytoskeleton TAS3056717
    GO:0016020membrane IEA--

    Find genes that share ontologies with MCF2           About GenesLikeMe


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
    About This Section

    TryGeneCards Plus

    SuperPaths for MCF2 About   (see all 11)  
    See pathways by source

    SuperPathContained pathways About
    1p75 NTR receptor-mediated signalling
    p75 NTR receptor-mediated signalling0.74
    NRAGE signals death through JNK0.71
    Cell death signalling via NRAGE, NRIF and NADE0.74
    G alpha (12/13) signalling events0.44
    2Signaling by GPCR
    Signaling by GPCR0.90
    Signal Transduction0.58
    GPCR downstream signaling0.90
    3Rho GTPase cycle
    Rho GTPase cycle1.00
    Signaling by Rho GTPases1.00
    4Signaling by FGFR
    Signalling by NGF0.71
    5Regulation of RhoA activity
    Regulation of RhoA activity


    Find genes that share SuperPaths with MCF2           About GenesLikeMe

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways


    1 Cell Signaling Technology (CST) Pathway for MCF2
        Cytoskeletal Signaling

    2 GeneGo (Thomson Reuters) Pathways for MCF2
        G-protein signaling Regulation of RAC1 activity
    G-protein signaling Regulation of CDC42 activity

    4 BioSystems Pathways for MCF2
        EGFR1 Signaling Pathway
    Regulation of CDC42 activity
    Regulation of RhoA activity
    Regulation of RAC1 activity

    3 Reactome Pathways for MCF2
        G alpha (12/13) signalling events
    NRAGE signals death through JNK
    Rho GTPase cycle


        Custom Pathway & Disease-focused RT2 Profiler PCR Arrays for MCF2
    Interactions:

        GeneGlobe Interaction Network for MCF2

    STRING Interaction Network Preview (showing 5 interactants - click image to see 17)

    Selected Interacting proteins for MCF2 (P109111, 2, 3 ENSP000003970554) via UniProtKB, MINT, STRING, and/or I2D (see all 17)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    RDXP352412, 3, ENSP000003428304MINT-17859 MINT-17858 I2D: score=1 STRING: ENSP00000342830
    NME1P155311, 3, ENSP000000130344EBI-1914514,EBI-741141 I2D: score=1 STRING: ENSP00000013034
    RHOAP615863, ENSP000004001754I2D: score=2 STRING: ENSP00000400175
    RHOGP840953, ENSP000003394674I2D: score=1 STRING: ENSP00000339467
    STUB1Q9UNE73, ENSP000002195484I2D: score=1 STRING: ENSP00000219548
    About this table

    Gene Ontology (GO): Selected biological process terms (see all 8):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0007264small GTPase mediated signal transduction TAS--
    GO:0016358dendrite development IEA--
    GO:0035023regulation of Rho protein signal transduction ----
    GO:0035556intracellular signal transduction ----
    GO:0043065positive regulation of apoptotic process TAS--

    Find genes that share ontologies with MCF2           About GenesLikeMe



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
    About This Section

    TryGeneCards Plus
    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Browse Tocris compounds for MCF2

    3 HMDB Compounds for MCF2    About this table
    CompoundSynonyms CAS #PubMed Ids
    Guanosine diphosphate5'-GDP (see all 10)146-91-8--
    Guanosine monophosphate5'-GMP (see all 14)85-32-5--
    Guanosine triphosphate5'-GTP (see all 10)86-01-1--

    Selected Novoseek inferred chemical compound relationships for MCF2 gene (see all 12)    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    gdp 81.3 22 9681826 (2), 8570171 (1), 8969170 (1), 10518015 (1) (see all 17)
    gtp 73.6 14 9681826 (2), 8969170 (1), 11007481 (1), 15817389 (1) (see all 12)
    guanine 39.4 1 12496381 (1)
    tyrosine 29.7 9 10652228 (5), 11007481 (2), 12496381 (1)
    arginine 16.6 6 11094164 (1), 15755723 (1), 19074425 (1), 12024019 (1) (see all 5)
    agar 6.74 1 10021386 (1)
    lipid 0 1 11577097 (1)
    calcium 0 2 9141492 (2)
    threonine 0 1 11337492 (1)
    estrogen 0 1 9627117 (1)



    Find genes that share compounds with MCF2           About GenesLikeMe



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, and/or Addgene, Primers from OriGene, and/or QIAGEN, Flow cytometry from eBioscience )
    About This Section

    TryGeneCards Plus

    REFSEQ mRNAs for MCF2 gene (6 alternative transcripts): 
    NM_001099855.1  NM_001171876.1  NM_001171877.1  NM_001171878.1  NM_001171879.1  NM_005369.4  

    Unigene Cluster for MCF2:

    MCF.2 cell line derived transforming sequence
    Hs.387262  [show with all ESTs]
    Unigene Representative Sequence: NM_001171876
    11 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000520602(uc011mwn.1) ENST00000437564 ENST00000370576(uc004fav.3 uc004fau.3 uc010nsh.2 uc011mwl.2)
    ENST00000446225 ENST00000519895 ENST00000370573 ENST00000338585 ENST00000483690
    ENST00000536274(uc011mwm.2) ENST00000370578 ENST00000414978(uc011mwo.1 uc004faw.2)

    miRNA
    Products:
         
    Block miRNA regulation of human, mouse, rat MCF2 using miScript Target Protectors
    6 qRT-PCR Assays for microRNAs that regulate MCF2:
    hsa-miR-124 hsa-miR-143 hsa-miR-506 hsa-miR-218 hsa-miR-636 hsa-miR-570
    SwitchGear 3'UTR luciferase reporter plasmidMCF2 3' UTR sequence
    Inhib. RNA
    Products:
         
    OriGene RNAi products in human, mouse, rat for MCF2
    Predesigned siRNA for gene silencing in human, mouse, rat MCF2
    Clone
    Products:
         
    OriGene clones in human, mouse for MCF2 (see all 28)
    OriGene ORF clones in mouse, rat for MCF2
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector (see all 6): MCF2 (NM_005369)
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for MCF2
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat MCF2
    Primer
    Products:
        
    OriGene qSTAR qPCR primer pairs in human, mouse for MCF2
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat MCF2
      QuantiTect SYBR Green Assays in human, mouse, rat MCF2
      QuantiFast Probe-based Assays in human, mouse, rat MCF2

    Additional mRNA sequence: 

    AB085901.1 AB085902.1 AK296488.1 AK296529.1 AK302957.1 AK313900.1 AK316416.1 AL117234.1 
    BC151247.1 J03639.1 X12556.1 X13230.1 

    5 DOTS entries:

    DT.95366728  DT.92070670  DT.92070671  DT.95366727  DT.121301235 

    24 AceView cDNA sequences:

    BX093553 AB085902 BQ632565 AL117234 AB085901 BQ632854 BQ189571 AL040486 
    AL120131 AL046410 AW341436 BG205068 X12556 NM_005369 BF062094 AV752834 
    X13230 N35192 AA610032 AI004109 J03639 N23406 AA758713 BU568480 

    GeneLoc Exon Structure


    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    TryGeneCards Plus

    MCF2 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: TTGTAATTCT
    MCF2 Expression
    About this image


    MCF2 expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database
     selected tissues (see all 3) fully expand
     
     Bone (Muscoskeletal System)    fully expand to see all 2 entries
             Zeugopod Long Bone
     
     Inner Cell Mass (Early Embryonic Tissues)
             Blimp1- mVenus and stella-ECFP genetically modified stem cells
     
     Testis (Reproductive System)
    MCF2 Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    MCF2 Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.387262

    UniProtKB/Swiss-Prot: MCF2_HUMAN, P10911
    Tissue specificity: Isoform 1 is expressed only in brain. Isoform 3 is expressed in heart, kidney, spleen, liver
    and testis. Isoform 4 is expressed in brain, heart, kidney, testis, placenta, stomach and peripheral blood. The
    protein is detectable in brain, heart, kidney, intestine, muscle, lung and testis

        Custom PCR Arrays for MCF2
    Primer
    Products:
    OriGene qSTAR qPCR primer pairs in human, mouse for MCF2
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat MCF2
    QuantiTect SYBR Green Assays in human, mouse, rat MCF2
    QuantiFast Probe-based Assays in human, mouse, rat MCF2
    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for MCF2

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    TryGeneCards Plus

    This gene was present in the common ancestor of animals.

    Orthologs for MCF2 gene from Selected species (see all 14)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Mcf21 , 5 mcf.2 transforming sequence1, 5 81.16(n)1
    76.05(a)1
      X (33.50 cM)5
    1099041  NM_133197.31  NP_573460.21 
     600559565 
    chicken
    (Gallus gallus)
    Aves MCF21 MCF.2 cell line derived transforming sequence 68.73(n)
    64.86(a)
      422253  XM_420239.4  XP_420239.4 
    lizard
    (Anolis carolinensis)
    Reptilia MCF26
    MCF.2 cell line derived transforming sequence
    57(a)
    1 ↔ 1
    GL343694.1(1608-69526)
    tropical clawed frog
    (Xenopus tropicalis)
    Amphibia mcf21 MCF.2 cell line derived transforming sequence 65.01(n)
    58.77(a)
      100489667  XM_002932628.2  XP_002932674.2 
    zebrafish
    (Danio rerio)
    Actinopterygii mcf2a1 MCF.2 cell line derived transforming sequence a 58.32(n)
    54.05(a)
      557201  XM_005173226.1  XP_005173283.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta CG304403 guanyl-nucleotide exchange factor 39(a)   41F4   --
    worm
    (Caenorhabditis elegans)
    Secernentea cgef-16
    Protein CGEF-1, isoform b (cgef-1) mRNA, complete ...
    21(a)
    1 → many
    X(2772042-2799243) WBGene00006496


    ENSEMBL Gene Tree for MCF2 (if available)
    TreeFam Gene Tree for MCF2 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section

    TryGeneCards Plus
    Paralogs for MCF2 gene
    KALRN2  ARHGEF252  MCF2L22  PLEKHG4B2  TRIO2  MCF2L2  KIAA17552  PLEKHG42  
    ARHGEF402  
    6 SIMAP similar genes for MCF2 using alignment to 4 protein entries:     MCF2_HUMAN (see all proteins):
    MCF2L    tgat    MCF2L2    KALRN    PREX2    TRIO

    Find genes that share paralogs with MCF2           About GenesLikeMe



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
    About This Section

    TryGeneCards Plus

    Selected SNPs for MCF2 (see all 1882)    About this table                                 

    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr X posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1509650691,2
    --138620755(+) AGCTAG/TCTATG 6 -- ds50010--------
    rs1839143081,2
    --138620821(+) GCATTA/CCCATG 6 -- ds50010--------
    rs66358861,2
    C,F,A,H--138620910(+) TTTGCT/CTGGAG 6 -- ds500110Minor allele frequency- C:0.01NS EA NA 1190
    rs1867666831,2
    --138621167(+) GAAAAC/TAGCAG 6 -- ut310--------
    rs1916746981,2
    --138621223(+) AAAAAA/TGTGTG 6 -- ut310--------
    rs1446403481,2
    C--138621298(+) ACAGAC/TAGTAG 6 -- ut310--------
    rs736340501,2
    C--138621793(+) CATTTC/GACATA 8 S * ut31 stg11Minor allele frequency- G:0.00WA 2
    rs1481147921,2
    C--138621813(+) TCAATA/GTAGGA 12 H Y mis1 syn11Minor allele frequency- G:0.00NA 4544
    rs1418711841,2
    F--138621814(+) CAATAT/CAGGAG 12 /L /Y /C syn1 mis11Minor allele frequency- C:0.00NA 4544
    rs1506387291,2
    C,F--138621839(+) CAGGTC/TTCTAC 12 K R N D mis11Minor allele frequency- T:0.00NA 4544

    HapMap Linkage Disequilibrium report for MCF2 (138663929 - 138790386 bp)

    Structural Variations
         Database of Genomic Variants (DGV) 3 variations for MCF2:    About this table    
    Variant IDTypeSubtypePubMed ID
    esv268694CNV Insertion20981092
    nsv523170CNV Gain19592680
    nsv528242CNV Gain19592680

    Human Gene Mutation Database (HGMD): MCF2
    Locus Specific Mutation Databases (LSDB): MCF2

    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing MCF2
    DNA2.0 Custom Variant and Variant Library Synthesis for MCF2

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    TryGeneCards Plus
    OMIM gene information: 311030    OMIM disorders: --

    UniProtKB/Swiss-Prot: MCF2_HUMAN, P10911
  • Note=MCF2 and DBL represent two activated versions of the same proto-oncogene

  • 2 diseases for MCF2:    
    About MalaCards
    aarskog-scott syndrome    scott syndrome

    2 diseases from the University of Copenhagen DISEASES database for MCF2:
    Aarskog-Scott syndrome     Malaria

    Find genes that share disorders with MCF2           About GenesLikeMe

    5 Novoseek inferred disease relationships for MCF2 gene    About this table

    Disease   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    lymphoma b-cell 11.5 2 15163014 (1), 15306850 (1)
    dysplasia 6.74 3 8969170 (1), 8994827 (1), 9671479 (1)
    leukemia 2.92 2 15276214 (1), 15163014 (1)
    tumors 0 1 2098495 (1)
    lymphoma 0 1 15163014 (1)

    Genetic Association Database (GAD): MCF2
    Tumor Gene Database (TGDB): MCF2

    Export disorders for MCF2 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    TryGeneCards Plus

    PubMed articles for MCF2 gene, integrated from 10 sources (see all 194):
    (articles sorted by number of sources associating them with MCF2)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Activation of a mcf.2 oncogene by deletion of amino-terminal coding sequences. (PubMed id 2577874)1, 2, 3 Noguchi T.... Birnbaum D. (Oncogene 1988)
    2. Activation of the guanine nucleotide exchange factor Dbl following ACK1-dependent tyrosine phosphorylation. (PubMed id 10652228)1, 2, 9 Kato J.... Satoh T. (Biochem. Biophys. Res. Commun. 2000)
    3. Alternative splicing variants of the human DBL (MCF-2) proto- oncogene. (PubMed id 12445822)1, 2, 9 Komai K.... Shiozawa S. (Biochem. Biophys. Res. Commun. 2002)
    4. Localization of the 5' end of the MCF2 oncogene to human chromosome 15q15----q23. (PubMed id 1611909)1, 3, 9 Galland F....Birnbaum D. (Cytogenet. Cell Genet. 1992)
    5. The DNA sequence of the human X chromosome. (PubMed id 15772651)1, 2 Ross M.T.... Bentley D.R. (Nature 2005)
    6. Cellular transformation and guanine nucleotide exchange activity are catalyzed by a common domain on the dbl oncogene product. (PubMed id 8276860)1, 2 Hart M.J.... Zheng Y. (J. Biol. Chem. 1994)
    7. A region of proto-dbl essential for its transforming activity shows sequence similarity to a yeast cell cycle gene, CDC24, and the human breakpoint cluster gene, bcr. (PubMed id 2065022)1, 2 Ron D.... Eva A. (New Biol. 1991)
    8. The predicted DBL oncogene product defines a distinct class of transforming proteins. (PubMed id 3281159)1, 2 Eva A.... Aaronson S.A. (Proc. Natl. Acad. Sci. U.S.A. 1988)
    9. Molecular cloning and characterization of the human dbl proto- oncogene: evidence that its overexpression is sufficient to transform NIH/3T3 cells. (PubMed id 3056717)1, 2 Ron D.... Eva A. (EMBO J. 1988)
    10. Characterization of novel splicing variants of the mouse MCF-2 (DBL) proto-oncogene. (PubMed id 13679059)1, 9 Komai K....Shiozawa S. (Biochem. Biophys. Res. Commun. 2003)

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

    TryGeneCards Plus
     ANDOR
    Aliases
    Disorders
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section

    TryGeneCards Plus
    Entrez Gene: 4168 HGNC: 6940 AceView: MCF2 Ensembl:ENSG00000101977 euGenes: HUgn4168
    ECgene: MCF2 H-InvDB: MCF2

    (According to HUGE)
    About This Section

    TryGeneCards Plus
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
    About This Section

    TryGeneCards Plus
    NameDescription
    PharmGKB entry for MCF2 Pharmacogenomics, SNPs, Pathways
    ATLAS Chromosomes in Cancer entry for MCF2 Genetics and Cytogenetics in Oncology and Haematology

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section

    TryGeneCards Plus
    Patent Information for MCF2 gene:
    Search GeneIP for patents involving MCF2

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, eBioscience, and/or others, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Addgene, Cell lines from GenScript, and ESI BIO, Flow cytometery from eBioscience, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
    Advanced Cell Diagnostics, Animal models from inGenious Targeting Laboratory, genOway)
    About This Section

    TryGeneCards Plus

     
     EMD Millipore genomic analysis products

      
     Browse Antibodies   Browse Cell Culture Products  
     Browse ELISAs   Browse Flow Cytometry Kits  
     Browse Primer Pairs   Browse Enzyme Activity Assays/Reagents  
     Browse ELISpot/FluoroSpot Kits/Development Modules   Browse TFB/Immunoprecipitation Assays  
     Browse Apoptosis Detection Kits/Reagents   Browse Ubiquitin Proteasome Pathway (UPP) Assay Kits/Reagents  
     Browse DNA Damage/Repair Kits/Reagents   Browse Luminex Assays  
     Browse Cell Selection/Detection Kits/Reagents   Browse Secondary Antibodies/Controls/Staining Reagents  
     Browse Recombinant/Natural Proteins   Browse Stem Cell Products  
     Browse cDNA Clones   Browse Proteome Profiler Antibody Arrays  
     Browse OriGene Antibodies   OriGene RNAi products in human, mouse, rat for MCF2  
     Browse OriGene qPCR primer pairs and template standards   OriGene Protein Over-expression Lysate for MCF2  
     OriGene Custom Mass Spec   OriGene clones in human, mouse for MCF2  
     OriGene qSTAR qPCR primer pairs in human, mouse for MCF2   Browse OriGene full length recombinant human proteins expressed in human HEK293 cells  
     OriGene ORF clones in mouse, rat for MCF2   OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling  
     OriGene Custom Antibody Services for MCF2   OriGene Custom Protein Services for MCF2  

     
     
     Block miRNA regulation of human, mouse, rat MCF2 using miScript Target Protectors SeqTarget long-range PCR primers for resequencing MCF2
     DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat MCF2 Predesigned siRNA for gene silencing in human, mouse, rat MCF2
     QuantiFast Probe-based Assays in human, mouse, rat MCF2 QuantiTect SYBR Green Assays in human, mouse, rat MCF2
     Custom PCR Arrays for MCF2 Search Chromatin IP Primers for MCF2
     Pre-validated RT2 qPCR Primer Assay in human, mouse, rat MCF2  GeneGlobe Interaction Network for MCF2
     Regulatory tfbs in MCF2 promoter
     GenScript Custom Purified and Recombinant Proteins Services for MCF2 GenScript cDNA clones with any tag delivered in your preferred vector for MCF2
     GenScript Custom Assay Services for MCF2 GenScript Custom overexpressing Cell Line Services for MCF2
     CloneReady with Over 120,000 Genes  Gene Synthesis: Any Gene in Any Vector
     Vector-based siRNA and miRNA, Ready for Transfection Gene Mutant Library, Variants up to 10^11
     Plasmid Preparation Custom Peptide Services
     Antibodies & Assays for MCF2 

     Search Tocris compounds for MCF2
     Browse Sino Biological Proteins
     Browse Sino Biological Cell Lysates
     Browse Sino Biological cDNA Clones
     4000+ Proteins
     Search Sino Biological for antibodies, proteins & pathways
     Protein Production Services
     Transfection Reagents
     Protein A/G/L resins
     Isotyping reagents
     Search www.enzolifesciences.com for proteins, assays, substrates, inhibitors & antibodies

     Novus Tissue Slides
     MCF2 antibodies
     Antibodies for MCF2
     See all of Abcam's Antibodies, Kits and Proteins for MCF2
     Custom Antibody / Protein Production Service
     Bulk Purchasing
     Advantages of Rabbit Monoclonal antibodies
     Abcam protocols and scientific support
     Browse ProSpec Recombinant Proteins
     Proteins for MCF2
     Antibodies for MCF2
     ELISAs for MCF2
     CLIAs for MCF2



     Browse ESI BIO Cell Lines and PureStem Progenitors for MCF2
     Gene Synthesis
     Protein Engineering
     Variant Library Synthesis
     Codon Optimization
     Protein Production and Purification
     Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for MCF2
     SwitchGear 3'UTR luciferase reporter plasmids for MCF2
     SwitchGear Promoter luciferase reporter plasmids for MCF2
     ThermoFisher Antibodies for MCF2
     Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat MCF2
     inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for MCF2
     inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for MCF2
     LSBio Antibodies in human, mouse, rat for MCF2
    Customized transgenic rodents for:
     Humanization
     Biomarker expression
     Off-target effect monitoring
     Translational medicine
     Tissue-specific gene expresssion
     Time-controlled gene expresssion
     Browse compounds at ApexBio
     Search Addgene for plasmids for MCF2
      Search eBioscience for proteins for MCF2
      Search eBioscience for elisas for MCF2
      eBioscience FlowRNA Probe Sets
           
    GeneCards Homepage - Last full update: 7 May 2014 - Incrementals: 9 May 2014 , 2 Jun 2014 , 26 Jun 2014 , 30 Jun 2014 , 21 Aug 2014 , 8 Sep 2014

    View Random Gene

    Category
    (GIFtS: )
    GIFtS Group
    The GeneCards human gene database gene index: 1 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 


    Developed at the Crown Human Genome Center, Department of Molecular Genetics, the Weizmann Institute of Science

    Hot genes      Disease genes      MCF2 gene at Home site.
    Version: 3.12.224 13 Oct 2014
    hostname: 356980-web2.xennexinc.com index build: 128 solr: 1.4