Set Analyses:
Advanced Search

Advanced Search

 
Search By
Section (entire)
for


 



MBD3 Gene

protein-coding   GIFtS: 64
GCID: GC19M001576

Methyl-CpG Binding Domain Protein 3

  See MBD3-related diseases
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
About This Section

TryGeneCards Plus

Aliases
Methyl-CpG Binding Domain Protein 31 2
Methyl-CpG-Binding Domain Protein 32
Methyl-CpG-Binding Protein MBD33

External Ids:    HGNC: 69181   Entrez Gene: 536152   Ensembl: ENSG000000716557   OMIM: 6035735   UniProtKB: O959833   

Export aliases for MBD3 gene to outside databases

Previous GC identifers: GC19M001639 GC19M001516 GC19M001527 GC19M001350


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

TryGeneCards Plus

Entrez Gene summary for MBD3 Gene:
DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian
development. This gene belongs to a family of nuclear proteins which are characterized by the presence of a
methyl-CpG binding domain (MBD). The encoded protein is a subunit of the NuRD, a multisubunit complex containing
nucleosome remodeling and histone deacetylase activities. Unlike the other family members, the encoded protein is
not capable of binding to methylated DNA. The protein mediates the association of metastasis-associated protein 2
with the core histone deacetylase complex. Alternative splicing results in multiple transcript variants of this
gene. (provided by RefSeq, Jul 2013)

GeneCards Summary for MBD3 Gene:
MBD3 (methyl-CpG binding domain protein 3) is a protein-coding gene. Diseases associated with MBD3 include cutaneous lupus erythematosus, and subacute cutaneous lupus erythematosus. GO annotations related to this gene include chromatin binding. An important paralog of this gene is MBD3L4.

UniProtKB/Swiss-Prot: MBD3_HUMAN, O95983
Function: Acts as transcriptional repressor and plays a role in gene silencing. Does not bind to DNA by itself
(PubMed:12124384). Binds to DNA with a preference for sites containing methylated CpG dinucleotides (in vitro).
Binds to a lesser degree DNA containing unmethylated CpG dinucleotides (PubMed:24307175). Recruits histone
deacetylases and DNA methyltransferases

Gene Wiki entry for MBD3 Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
About This Section

TryGeneCards Plus
RefSeq DNA sequence at NCBI GenBank:
NC_000019.9  NC_018930.2  NT_011295.12  
Regulatory elements:
   Regulatory transcription factor binding sites in the MBD3 gene promoter:
         Elk-1   RP58   XBP-1   CBF-C   Egr-4   CBF-B   CBF-A   c-Myb   STAT3   CBF(2)   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidMBD3 promoter sequence
   Search Chromatin IP Primers for MBD3

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat MBD3


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 19p13.3   Ensembl cytogenetic band:  19p13.3   HGNC cytogenetic band: 19p13

MBD3 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
MBD3 gene location

GeneLoc information about chromosome 19         GeneLoc Exon Structure

GeneLoc location for GC19M001576:  view genomic region     (about GC identifiers)

Start:
1,576,639 bp from pter      End:
1,592,882 bp from pter
Size:
16,244 bases      Orientation:
minus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, Cloud-Clone Corp., and/or eBioscience,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, Cloud-Clone Corp., and/or eBioscience, Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, Cloud-Clone Corp, and/or others.)
About This Section

TryGeneCards Plus

UniProtKB/Swiss-Prot: MBD3_HUMAN, O95983 (See protein sequence)
Recommended Name: Methyl-CpG-binding domain protein 3  
Size: 291 amino acids; 32844 Da
Subunit: Heterodimer with MBD2. Part of the NuRD and the MeCP1 complex. Interacts with BCL6, HDAC1, MTA2, DNMT1,
p66-alpha and p66-beta
Sequence caution: Sequence=AAH32443.1; Type=Erroneous initiation; Note=Translation N-terminally shortened;
1 PDB 3D structure from and Proteopedia for MBD3:
2MB7 (3D)    
Secondary accessions: A8K4B7 D6W5Z2 Q6PIL9 Q6PJZ9 Q86XF4
Alternative splicing: 2 isoforms:  O95983-1   O95983-2   (No experimental confirmation available)

Explore the universe of human proteins at neXtProt for MBD3: NX_O95983

Explore proteomics data for MBD3 at MOPED

Post-translational modifications: 

  • Modification sites at neXtProt
  • Modification sites at PhosphoSitePlus

  • See MBD3 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins (2 alternative transcripts): 
    NP_001268382.1  NP_001268383.1  

    ENSEMBL proteins: 
     ENSP00000464718   ENSP00000412302   ENSP00000466670   ENSP00000156825  
    Reactome Protein details: O95983

    MBD3 Human Recombinant Protein Products:

    Browse Purified and Recombinant Proteins at EMD Millipore
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Browse OriGene full length recombinant human proteins expressed in human HEK293 cells
    OriGene Protein Over-expression Lysate for MBD3
    OriGene Custom MassSpec
    OriGene Custom Protein Services for MBD3
    GenScript Custom Purified and Recombinant Proteins Services for MBD3
    Novus Biologicals MBD3 Proteins
    Novus Biologicals MBD3 Lysate
    Browse Sino Biological Recombinant Proteins
    Browse Sino Biological Cell Lysates
    ProSpec Recombinant Protein for MBD3
    Cloud-Clone Corp. Proteins for MBD3

     
    Search eBioscience for Proteins for MBD3 

    MBD3 Antibody Products:

    EMD Millipore Mono- and Polyclonal Antibodies for the study of MBD3
    R&D Systems Antibodies for MBD3
    Cell Signaling Technology (CST) Antibodies for MBD3 
    OriGene Antibodies for MBD3
    OriGene Custom Antibody Services for MBD3
    Novus Biologicals MBD3 Antibodies
    Abcam antibodies for MBD3
    Cloud-Clone Corp. Antibodies for MBD3
    ThermoFisher Antibodies for MBD3
    LSBio Antibodies in human, mouse, rat for MBD3

    MBD3 Assay Products:

    Browse Kits and Assays available from EMD Millipore
    OriGene Custom Assay Services for MBD3
    Browse R&D Systems for biochemical assays
    GenScript Custom Assay Services for MBD3
    Browse Enzo Life Sciences for kits & assays
    Cloud-Clone Corp. ELISAs for MBD3
    Cloud-Clone Corp. CLIAs for MBD3
    Search eBioscience for ELISAs for MBD3 


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    TryGeneCards Plus
    3 InterPro protein domains:
     IPR025884 MeCpG-bd_2/3_C_dom
     IPR001739 Methyl_CpG_DNA-bd
     IPR016177 DNA-bd_dom

    Graphical View of Domain Structure for InterPro Entry O95983

    ProtoNet protein and cluster: O95983

    1 Blocks protein domain: IPB001739 Methyl-CpG binding

    UniProtKB/Swiss-Prot: MBD3_HUMAN, O95983
    Similarity: Contains 1 MBD (methyl-CpG-binding) domain


    Find genes that share domains with MBD3           About GenesLikeMe


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, Vector BioLabs and/or Addgene, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Flow cytometry from eBioscience, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
    About This Section

    TryGeneCards Plus

    Molecular Function:

         UniProtKB/Swiss-Prot Summary: MBD3_HUMAN, O95983
    Function: Acts as transcriptional repressor and plays a role in gene silencing. Does not bind to DNA by itself
    (PubMed:12124384). Binds to DNA with a preference for sites containing methylated CpG dinucleotides (in vitro).
    Binds to a lesser degree DNA containing unmethylated CpG dinucleotides (PubMed:24307175). Recruits histone
    deacetylases and DNA methyltransferases

         Genatlas biochemistry entry for MBD3:
    methyl-CpG binding protein 3,expressed in somatic tissues,not binding methylated DNA in vitro or in vivo

         Gene Ontology (GO): 5 molecular function terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003677DNA binding TAS9774669
    GO:0003682chromatin binding IEA--
    GO:0005488binding ----
    GO:0005515protein binding IPI12183469
    GO:0008327methyl-CpG binding IDA--
         
    Find genes that share ontologies with MBD3           About GenesLikeMe


    Phenotypes:
         1 GenomeRNAi human phenotype for MBD3:
     Decreased TP53 protein express 

         6 MGI mutant phenotypes (inferred from 3 alleles(MGI details for Mbd3):
     cellular  digestive/alimentary  homeostasis/metabolism  immune system  mortality/aging 
     tumorigenesis 

    Find genes that share phenotypes with MBD3           About GenesLikeMe

    Animal Models:
       inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for MBD3
       inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for MBD3

       genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for MBD3
       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for MBD3

    miRNA
    Products:
        
    miRTarBase miRNAs that target MBD3:
    hsa-mir-124-3p (MIRT022788), hsa-mir-877-3p (MIRT037081)

    Block miRNA regulation of human, mouse, rat MBD3 using miScript Target Protectors
    Search for qRT-PCR Assays for microRNAs that regulate MBD3
    SwitchGear 3'UTR luciferase reporter plasmidMBD3 3' UTR sequence
    Inhib. RNA
    Products:
        
    OriGene RNAi products in human, mouse, rat for MBD3
    Predesigned siRNA for gene silencing in human, mouse, rat MBD3

    Gene Editing
    Products:
    DNA2.0 Custom Protein Engineering Service for MBD3

    Clone
    Products:
         
    OriGene clones in human, mouse for MBD3 (see all 6)
    OriGene ORF clones in mouse, rat for MBD3
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector: MBD3 (NM_003926)
    Browse Sino Biological Human cDNA Clones
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for MBD3
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat MBD3
    Addgene plasmids for MBD3 

    Cell Line
    Products:
         
    GenScript Custom overexpressing Cell Line Services for MBD3
    Browse ESI BIO Cell Lines and PureStem Progenitors for MBD3 
    In Situ Assay
    Products:
       

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for MBD3


    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
    About This Section

    TryGeneCards Plus

    Subcellular locations from UniProtKB/Swiss-Prot
    MBD3_HUMAN, O95983: Nucleus. Chromosome. Note=Nuclear, in discrete foci. Detected on chromatin, at promoter
    regions of active genes
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    nucleus5
    cytosol2

    Gene Ontology (GO): Selected cellular component terms (see all 6):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000785chromatin ----
    GO:0000792heterochromatin IEA--
    GO:0005634nucleus ----
    GO:0005737cytoplasm IEA--
    GO:0016581NuRD complex NAS17827154

    Find genes that share ontologies with MBD3           About GenesLikeMe


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
    About This Section

    TryGeneCards Plus

    SuperPaths for MBD3 About   (see all 7)  
    See pathways by source

    SuperPathContained pathways About
    1RNA Polymerase I Promoter Opening
    RNA Polymerase I Transcription0.78
    RNA Polymerase I, RNA Polymerase III, and Mitochondrial Transcription0.73
    RNA Polymerase I Promoter Clearance0.78
    2RNA Polymerase I Promoter Escape
    RNA Polymerase I Transcription Initiation0.55
    3Gene Expression
    Gene Expression0.40
    4Development NOTCH1 mediated pathway for NF KB activity modulation
    Transcription Sin3 and NuRD in transcription regulation0.35
    5Macrophage Differentiation and Growth Inhibition by METS
    DNA Methylation and Transcriptional Repression0.30


    Find genes that share SuperPaths with MBD3           About GenesLikeMe

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways

    1 Downloadable PowerPoint Slide of GeneGlobe Pathway Central Maps for MBD3
        DNA Methylation and Transcriptional Repression

    1 Cell Signaling Technology (CST) Pathway for MBD3
        Chromatin Regulation / Acetylation

    1 GeneGo (Thomson Reuters) Pathway for MBD3
        Transcription Sin3 and NuRD in transcription regulation

    1 BioSystems Pathway for MBD3
        Signaling events mediated by HDAC Class I

    1 Reactome Pathway for MBD3
        RNA Polymerase I Transcription Initiation


        Pathway & Disease-focused RT2 Profiler PCR Array including MBD3: 
              Epigenetic Chromatin Remodeling Factors in human mouse rat

    Interactions:

        GeneGlobe Interaction Network for MBD3

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    Selected Interacting proteins for MBD3 (O959831, 2, 3 ENSP000001568254) via UniProtKB, MINT, STRING, and/or I2D (see all 665)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    DDX5P178441, 2, 3, ENSP000002257924EBI-1783068,EBI-351962 MINT-7945693 I2D: score=3 STRING: ENSP00000225792
    MTA2O947762, 3, ENSP000002788234MINT-7945693 MINT-7943765 I2D: score=5 STRING: ENSP00000278823
    HDAC1Q135472, 3, ENSP000003626494MINT-7945693 MINT-7943765 I2D: score=4 STRING: ENSP00000362649
    HDAC2Q927692, 3, ENSP000003813314MINT-7945693 MINT-7943765 I2D: score=2 STRING: ENSP00000381331
    AURKAO149652, 3, ENSP000002169114MINT-7945693 I2D: score=3 STRING: ENSP00000216911
    About this table

    Gene Ontology (GO): Selected biological process terms (see all 6):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000122negative regulation of transcription from RNA polymerase II promoter IEA--
    GO:0001701in utero embryonic development IEA--
    GO:0006346methylation-dependent chromatin silencing IEA--
    GO:0006351transcription, DNA-templated IEA--
    GO:0009888tissue development IEA--

    Find genes that share ontologies with MBD3           About GenesLikeMe



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
    About This Section

    TryGeneCards Plus
    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Browse Tocris compounds for MBD3



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, and/or Addgene, Primers from OriGene, and/or QIAGEN, Flow cytometry from eBioscience )
    About This Section

    TryGeneCards Plus

    REFSEQ mRNAs for MBD3 gene (3 alternative transcripts): 
    NM_001281453.1  NM_001281454.1  NM_003926.5  

    Unigene Cluster for MBD3:

    Methyl-CpG binding domain protein 3
    Hs.178728  [show with all ESTs]
    Unigene Representative Sequence: NM_003926
    11 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000590550 ENST00000434436(uc002ltk.3) ENST00000592012 ENST00000590830
    ENST00000589901 ENST00000585903 ENST00000585967 ENST00000589064 ENST00000592361
    ENST00000592965 ENST00000156825(uc002ltj.3 uc002ltl.1)
    miRNA
    Products:
         
    Block miRNA regulation of human, mouse, rat MBD3 using miScript Target Protectors
    Search for qRT-PCR Assays for microRNAs that regulate MBD3
    SwitchGear 3'UTR luciferase reporter plasmidMBD3 3' UTR sequence
    Inhib. RNA
    Products:
         
    OriGene RNAi products in human, mouse, rat for MBD3
    Predesigned siRNA for gene silencing in human, mouse, rat MBD3
    Clone
    Products:
         
    OriGene clones in human, mouse for MBD3 (see all 6)
    OriGene ORF clones in mouse, rat for MBD3
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector: MBD3 (NM_003926)
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for MBD3
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat MBD3
    Addgene plasmids for MBD3 
    Primer
    Products:
        
    OriGene qPCR primer pairs and template standards for MBD3
    OriGene qSTAR qPCR primer pairs in human, mouse for MBD3
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat MBD3
      QuantiTect SYBR Green Assays in human, mouse, rat MBD3
      QuantiFast Probe-based Assays in human, mouse, rat MBD3

    Additional mRNA sequence: 

    AF072247.1 AK001474.1 AK021532.1 AK290882.1 BC000872.1 BC009372.1 BC009438.1 BC018784.1 
    BC032443.1 BC043619.1 X58233.1 

    21 DOTS entries:

    DT.100034143  DT.65284492  DT.101978021  DT.100819819  DT.100819817  DT.100806804  DT.100819820  DT.121471043 
    DT.75138848  DT.100819822  DT.91823425  DT.100797144  DT.95211055  DT.100708644  DT.121470970  DT.86836938 
    DT.92443555  DT.95210998  DT.100037882  DT.91997741  DT.97859994 

    Selected AceView cDNA sequences (see all 851):

    AA588259 NM_006830 BF939827 BX380183 BM042169 AA427497 AW163325 BQ066010 
    AA580639 AW205720 AA715239 BF219927 BF772409 CR605036 CB152093 BU181763 
    BQ680714 BQ027245 AI123794 BQ951728 CF528536 AI420139 AI372848 BM783477 

    GeneLoc Exon Structure

    Selected Alternative Splicing Database (ASD) splice patterns (SP) for MBD3 (see all 8)    About this scheme

    ExUns: 1a · 1b · 1c ^ 2 ^ 3 ^ 4a · 4b · 4c ^ 5a · 5b · 5c ^ 6 ^ 7 ^ 8a · 8b · 8c ^ 9a · 9b · 9c
    SP1:              -     -     -     -     -           -                 -                                             
    SP2:                    -     -     -     -           -                 -                                             
    SP3:                                                  -                 -                                             
    SP4:                                                  -                 -                                             
    SP5:                          -     -     -           -                 -                                             


    ECgene alternative splicing isoforms for MBD3

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    TryGeneCards Plus

    MBD3 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: GGTGGATGTG
    MBD3 Expression
    About this image


    MBD3 expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database
     selected tissues (see all 4) fully expand
     
     Heart (Cardiovascular System)    fully expand to see all 2 entries
             Atrioventricular Canal Cells Atrioventricular Canal
             Outflow Tract
     
     Brain (Nervous System)    fully expand to see all 2 entries
             Cerebral Cortex
     
     Trophoblast (Extraembryonic Tissues)
             Trophoblast Cells Trophoblast
     
     Bone (Muscoskeletal System)
             Bone Marrow
    MBD3 Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    MBD3 Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.178728
        Pathway & Disease-focused RT2 Profiler PCR Array including MBD3: 
              Epigenetic Chromatin Remodeling Factors in human mouse rat

    Primer
    Products:
    OriGene qPCR primer pairs and template standards for MBD3
    OriGene qSTAR qPCR primer pairs in human, mouse for MBD3
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat MBD3
    QuantiTect SYBR Green Assays in human, mouse, rat MBD3
    QuantiFast Probe-based Assays in human, mouse, rat MBD3
    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for MBD3

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    TryGeneCards Plus

    This gene was present in the common ancestor of animals.

    Orthologs for MBD3 gene from Selected species (see all 13)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Mbd31 , 5 methyl-CpG binding domain protein 31, 5 89.14(n)1
    97.75(a)1
      10 (39.72 cM)5
    171921  NM_013595.21  NP_038623.11 
     803925395 
    chicken
    (Gallus gallus)
    Aves MBD31 methyl-CpG binding domain protein 3 82.37(n)
    96.15(a)
      770153  NM_001079751.2  NP_001073219.1 
    African clawed frog
    (Xenopus laevis)
    Amphibia MBD32 methyl-CpG binding protein MBD3 long form 76.44(n)    AF170346.1 
    zebrafish
    (Danio rerio)
    Actinopterygii Dr.284072 Danio rerio methyl-CpG binding protein 3a (mbd3a) mRNA, more 79.83(n)    AY398379.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta MBD-like6
    MBD-like
    22(a)
    1 → many
    3R(5385487-5387255)


    ENSEMBL Gene Tree for MBD3 (if available)
    TreeFam Gene Tree for MBD3 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section

    TryGeneCards Plus
    Paralogs for MBD3 gene
    MBD3L42  MBD22  MBD3L32  MBD3L12  MBD3L52  MBD3L22  
    6 SIMAP similar genes for MBD3 using alignment to 2 protein entries:     MBD3_HUMAN (see all proteins):
    MBD2    DKFZp586O0821    NY-CO-41    MBD3L1    MBD3L2    MBD3L5

    Find genes that share paralogs with MBD3           About GenesLikeMe



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
    About This Section

    TryGeneCards Plus

    Selected SNPs for MBD3 (see all 479)    About this table                                 

    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 19 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1393288381,2
    C--1368599(+) AATTAC/G/TGTTTT 1 -- us2k10--------
    rs1179372481,2
    C,F--1505032(+) TGCAGC/TGGCTG 1 -- ds50011Minor allele frequency- T:0.04NA 120
    rs1866021161,2
    --1505110(+) GGAGCA/GGTGCT 1 -- ds50010--------
    rs1508797321,2
    --1505205(+) GGGCAC/TGGCCC 1 -- ds50010--------
    rs1918208601,2
    --1505206(+) GGCACA/GGCCCT 1 -- ds50010--------
    rs1840397941,2
    --1505223(+) CAGATA/GCCCAG 1 -- ds50010--------
    rs1393332551,2
    --1505232(+) AGGCCA/GGCCTG 1 -- ds50010--------
    rs1158448191,2
    --1505322(+) ACACCA/GCTTTC 1 -- ds50010--------
    rs1445284011,2
    --1505392(+) CGCACC/TGAACT 1 -- ds50010--------
    rs1129808841,2
    C,F--1505393(+) GCACCG/CAACTC 1 -- ds50012Minor allele frequency- C:0.02CSA WA 120

    HapMap Linkage Disequilibrium report for MBD3 (1576639 - 1592882 bp)

    Structural Variations
         Database of Genomic Variants (DGV) Selected variations for MBD3 (see all 16):    About this table    
    Variant IDTypeSubtypePubMed ID
    dgv3589n71CNV Loss21882294
    dgv3608n71CNV Loss21882294
    dgv3607n71CNV Loss21882294
    dgv3601n71CNV Loss21882294
    dgv3611n71CNV Loss21882294
    nsv910292CNV Loss21882294
    nsv910540CNV Loss21882294
    dgv3610n71CNV Loss21882294
    dgv3603n71CNV Loss21882294
    nsv910499CNV Loss21882294

    Human Gene Mutation Database (HGMD): MBD3
    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing MBD3
    DNA2.0 Custom Variant and Variant Library Synthesis for MBD3

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    TryGeneCards Plus
    OMIM gene information: 603573    OMIM disorders: --

    3 diseases for MBD3:    
    About MalaCards
    cutaneous lupus erythematosus    subacute cutaneous lupus erythematosus    silver-russell syndrome


    Find genes that share disorders with MBD3           About GenesLikeMe


    Export disorders for MBD3 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    TryGeneCards Plus

    PubMed articles for MBD3 gene, integrated from 10 sources (see all 91):
    (articles sorted by number of sources associating them with MBD3)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Identification and characterization of a family of mammalian methyl- CpG binding proteins. (PubMed id 9774669)1, 2, 3, 9 Hendrich B. and Bird A. (Mol. Cell. Biol. 1998)
    2. Genomic structure and chromosomal mapping of the murine and human mbd1, mbd2, mbd3, and mbd4 genes. (PubMed id 10441743)1, 3, 9 Hendrich B.... Bird A. (Mamm. Genome 1999)
    3. Differential roles for MBD2 and MBD3 at methylated CpG islands, active promoters and binding to exon sequences. (PubMed id 23361464)1, 2 GA1nther K....Renkawitz R. (Nucleic Acids Res. 2013)
    4. MBD3, a component of the NuRD complex, facilitates chromatin alteration and deposition of epigenetic marks. (PubMed id 18644863)1, 2 Morey L....Di Croce L. (Mol. Cell. Biol. 2008)
    5. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (Nat. Genet. 2004)
    6. MTA3 and the Mi-2/NuRD complex regulate cell fate during B lymphocyte differentiation. (PubMed id 15454082)1, 2 Fujita N....Wade P.A. (Cell 2004)
    7. The DNA sequence and biology of human chromosome 19. (PubMed id 15057824)1, 2 Grimwood J.... Lucas S.M. (Nature 2004)
    8. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S.... Malek J. (Genome Res. 2004)
    9. Identification and functional characterization of the p66/p68 components of the MeCP1 complex. (PubMed id 11756549)1, 2 Feng Q.... Zhang Y. (Mol. Cell. Biol. 2002)
    10. The mCpG-binding domain of human MBD3 does not bind to mCpG but interacts with NuRD/Mi2 components HDAC1 and MTA2. (PubMed id 12124384)1, 2 Saito M. and Ishikawa F. (J. Biol. Chem. 2002)

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

    TryGeneCards Plus
     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section

    TryGeneCards Plus
    Entrez Gene: 53615 HGNC: 6918 AceView: MBD3andUQCR Ensembl:ENSG00000071655 euGenes: HUgn53615
    ECgene: MBD3 H-InvDB: MBD3

    (According to HUGE)
    About This Section

    TryGeneCards Plus
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
    About This Section

    TryGeneCards Plus
    NameDescription
    PharmGKB entry for MBD3 Pharmacogenomics, SNPs, Pathways
    ATLAS Chromosomes in Cancer entry for MBD3 Genetics and Cytogenetics in Oncology and Haematology

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section

    TryGeneCards Plus
    Patent Information for MBD3 gene:
    Search GeneIP for patents involving MBD3

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, eBioscience, and/or others, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Addgene, Cell lines from GenScript, and ESI BIO, Flow cytometery from eBioscience, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
    Advanced Cell Diagnostics, Animal models from inGenious Targeting Laboratory, genOway)
    About This Section

    TryGeneCards Plus

     Browse Kits and Assays available from EMD Millipore
     Browse Small Molecules at EMD Millipore
     EMD Millipore Mono- and Polyclonal Antibodies for the study of MBD3
     Browse Purified and Recombinant Proteins at EMD Millipore
     
     EMD Millipore genomic analysis products

      
     Antibodies for MBD3 (MBD3)   Browse Cell Culture Products  
     Browse ELISAs   Browse Flow Cytometry Kits  
     Browse Primer Pairs   Browse Enzyme Activity Assays/Reagents  
     Browse ELISpot/FluoroSpot Kits/Development Modules   Browse TFB/Immunoprecipitation Assays  
     Browse Apoptosis Detection Kits/Reagents   Browse Ubiquitin Proteasome Pathway (UPP) Assay Kits/Reagents  
     Browse DNA Damage/Repair Kits/Reagents   Browse Luminex Assays  
     Browse Cell Selection/Detection Kits/Reagents   Browse Secondary Antibodies/Controls/Staining Reagents  
     Browse Recombinant/Natural Proteins   Browse Stem Cell Products  
     Browse cDNA Clones   Browse Proteome Profiler Antibody Arrays  
     OriGene Antibodies for MBD3   OriGene RNAi products in human, mouse, rat for MBD3  
     OriGene qPCR primer pairs and template standards for MBD3   OriGene Protein Over-expression Lysate for MBD3  
     OriGene Custom Mass Spec   OriGene clones in human, mouse for MBD3  
     OriGene qSTAR qPCR primer pairs in human, mouse for MBD3   Browse OriGene full length recombinant human proteins expressed in human HEK293 cells  
     OriGene ORF clones in mouse, rat for MBD3   OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling  
     OriGene Custom Antibody Services for MBD3   OriGene Custom Protein Services for MBD3  

     
     
     Block miRNA regulation of human, mouse, rat MBD3 using miScript Target Protectors SeqTarget long-range PCR primers for resequencing MBD3
     DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat MBD3 Predesigned siRNA for gene silencing in human, mouse, rat MBD3
     QuantiFast Probe-based Assays in human, mouse, rat MBD3 QuantiTect SYBR Green Assays in human, mouse, rat MBD3
     PCR Arrays including human, mouse, rat MBD3 Search Chromatin IP Primers for MBD3
     Pre-validated RT2 qPCR Primer Assay in human, mouse, rat MBD3  GeneGlobe Interaction Network for MBD3
     Regulatory tfbs in MBD3 promoter
     GenScript Custom Purified and Recombinant Proteins Services for MBD3 GenScript cDNA clones with any tag delivered in your preferred vector for MBD3
     GenScript Custom Assay Services for MBD3 GenScript Custom overexpressing Cell Line Services for MBD3
     CloneReady with Over 120,000 Genes  Gene Synthesis: Any Gene in Any Vector
     Vector-based siRNA and miRNA, Ready for Transfection Gene Mutant Library, Variants up to 10^11
     Plasmid Preparation Custom Peptide Services
     Antibodies & Assays for MBD3 

     Search Tocris compounds for MBD3
     Browse Sino Biological Proteins
     Browse Sino Biological Cell Lysates
     Browse Sino Biological cDNA Clones
     4000+ Proteins
     Search Sino Biological for antibodies, proteins & pathways
     Protein Production Services
     Transfection Reagents
     Protein A/G/L resins
     Isotyping reagents
     Search www.enzolifesciences.com for proteins, assays, substrates, inhibitors & antibodies

     Novus Tissue Slides
     MBD3 antibodies
     MBD3 proteins
     MBD3 lysates
     Antibodies for MBD3
     See all of Abcam's Antibodies, Kits and Proteins for MBD3
     Custom Antibody / Protein Production Service
     Bulk Purchasing
     Advantages of Rabbit Monoclonal antibodies
     Abcam protocols and scientific support
     Recombinant Protein for MBD3
     Proteins for MBD3
     Antibodies for MBD3
     ELISAs for MBD3
     CLIAs for MBD3



     Browse ESI BIO Cell Lines and PureStem Progenitors for MBD3
     Gene Synthesis
     Protein Engineering
     Variant Library Synthesis
     Codon Optimization
     Protein Production and Purification
     Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for MBD3
     SwitchGear 3'UTR luciferase reporter plasmids for MBD3
     SwitchGear Promoter luciferase reporter plasmids for MBD3
     ThermoFisher Antibodies for MBD3
     Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat MBD3
     inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for MBD3
     inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for MBD3
     LSBio Antibodies in human, mouse, rat for MBD3
    Customized transgenic rodents for:
     Humanization
     Biomarker expression
     Off-target effect monitoring
     Translational medicine
     Tissue-specific gene expresssion
     Time-controlled gene expresssion
     Browse compounds at ApexBio
     Addgene plasmids for MBD3
      Search eBioscience for proteins for MBD3
      Search eBioscience for elisas for MBD3
      eBioscience FlowRNA Probe Sets
           
    GeneCards Homepage - Last full update: 7 May 2014 - Incrementals: 9 May 2014 , 2 Jun 2014 , 26 Jun 2014 , 30 Jun 2014 , 21 Aug 2014 , 8 Sep 2014

    View Random Gene

    Category
    (GIFtS: )
    GIFtS Group
    The GeneCards human gene database gene index: 1 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 


    Developed at the Crown Human Genome Center, Department of Molecular Genetics, the Weizmann Institute of Science

    Hot genes      Disease genes      MBD3 gene at Home site.
    Version: 3.12.212 17 Sep 2014
    hostname: 356980-web2.xennexinc.com index build: 128 solr: 1.4