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Category Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21

GIFtS
-

MARK2 Gene

protein-coding GIFtS: 62
GCID: GC11P063606

MAP/microtubule affinity-regulating kinase 2

(Previous name: ELKL motif kinase )
(Previous symbol: EMK1)

Explore 8 diseases affiliated with
MARK2 via

MalaCards

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Human Malady Compendium

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc, ⁷Ensembl, ⁸DME, ⁹miRBase, and/or ¹⁰fRNAdb)
About This Section

Aliases
MAP/Microtubule Affinity-Regulating Kinase 2¹ ² ³		EC 2.7.11.1³ ⁸
EMK1¹ ² ³ ⁵		ELKL Motif Kinase¹
Par1b¹ ² ³		Ser/Thr Protein Kinase PAR-1B²
PAR-1¹ ²		Serine/Threonine Protein Kinase EMK²
EMK-1² ³		Serine/Threonine-Protein Kinase MARK2²
Par-1b² ³		EC 2.7.11.26³
ELKL Motif Kinase 1² ³		PAR1 Homolog³
PAR1 Homolog B² ³		EC 2.7.11⁸

External Ids:	HGNC: 3332¹	Entrez Gene: 2011²	Ensembl: ENSG00000072518⁷	OMIM: 600526⁵	UniProtKB: Q7KZI7³

Export aliases for MARK2 gene to outside databases

Previous GC identifers: GC11P066121 GC11P065286 GC11P063857 GC11P063382 GC11P063431 GC11P063363 GC11P059934

(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for MARK2:

This gene encodes a member of the Par-1 family of serine/threonine protein kinases. The protein is an important

regulator of cell polarity in epithelial and neuronal cells, and also controls the stability of microtubules through

phosphorylation and inactivation of several microtubule-associating proteins. The protein localizes to cell membranes.

Multiple transcript variants encoding different isoforms have been found for this gene. (provided by RefSeq, Jul 2009)

UniProtKB/Swiss-Prot: MARK2_HUMAN, Q7KZI7

Function: Serine/threonine-protein kinase involved in cell polarity and microtubule dynamics regulation. Phosphorylates

CRTC2/TORC2, DCX, HDAC7, KIF13B, MAP2, MAP4, MAPT/TAU, and RAB11FIP2. Plays a key role in cell polarity by

phosphorylating the microtubule-associated proteins MAP2, MAP4 and MAPT/TAU at KXGS motifs, causing detachment from

microtubules, and their disassembly. Regulates epithelial cell polarity by phosphorylating RAB11FIP2. Involved in the

regulation of neuronal migration through its dual activities in regulating cellular polarity and microtubule dynamics,

possibly by phosphorylating and regulating DCX. Regulates axogenesis by phosphorylating KIF13B, promoting interaction

between KIF13B and 14-3-3 and inhibiting microtubule-dependent accumulation of KIF13B. Also required for neurite

outgrowth and establishment of neuronal polarity. Regulates localization and activity of some histone deacetylases by

mediating phosphorylation of HDAC7, promoting subsequent interaction between HDAC7 and 14-3-3 and export from the

nucleus. Also acts as a positive regulator of the Wnt signaling pathway, probably by mediating phosphorylation of

dishevelled proteins (DVL1, DVL2 and/or DVL3). Modulates the developmental decision to build a columnar versus a

hepatic epithelial cell apparently by promoting a switch from a direct to a transcytotic mode of apical protein

delivery. Essential for the asymmetric development of membrane domains of polarized epithelial cells

Gene Wiki entry for MARK2

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section

RefSeq DNA sequence:

NC_000011.9 NC_018922.1 NT_167190.1

Regulatory elements:

SABiosciences Regulatory transcription factor binding sites in the MARK2 gene promoter:
PPAR-gamma1 AP-2alpha isoform 3 PPAR-gamma2 AP-2alpha isoform 2 AP-2alpha isoform 4 AP-2alpha AP-2alphaA
Other transcription factors

SwitchGear Promoter luciferase reporter plasmids (see all 2): MARK2 promoter sequence

Search SABiosciences Chromatin IP Primers for MARK2

Epigenetics:

QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat MARK2

Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 11q13.1 Ensembl cytogenetic band: 11q13.1 HGNC cytogenetic band: 11q13.1

MARK2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
MARK2 gene location

GeneLoc information about chromosome 11 GeneLoc Exon Structure

GeneLoc location for GC11P063606: view genomic region (about GC identifiers)

Start:	63,606,400 bp from pter	End:	63,678,492 bp from pter
Size:	72,093 bases	Orientation:	plus strand

(According to ¹UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to ²PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: MARK2_HUMAN, Q7KZI7 (See protein sequence)

Recommended Name: Serine/threonine-protein kinase MARK2

Size: 788 amino acids; 87911 Da

Cofactor: Magnesium (By similarity)

Subunit: Homodimer. Interacts with PAK7/PAK5; leading to inhibit the protein kinase activity (By similarity). Interacts

(when phosphorylated at Thr-596) with YWHAZ. In case of infection, interacts with H.pylori CagA, leading to inhibit

kinase activity and junctional and polarity defects

Subcellular location: Cell membrane; Peripheral membrane protein. Cytoplasm. Note=Phosphorylation at Thr-596 by

PRKCZ/aPKC and subsequent interaction with 14-3-3 protein YWHAZ promotes relocation from the cell membrane to the

cytoplasm

Sequence caution: Sequence=AAK82368.1; Type=Erroneous initiation; Note=Translation N-terminally extended;

1 PDB 3D structure from and Proteopedia for MARK2:

3IEC (3D)

Secondary accessions: Q15449 Q15524 Q5XGA3 Q68A18 Q96HB3 Q96RG0

Alternative promoter usage, Alternative splicing: 16 isoforms: Q7KZI7-1 Q7KZI7-2 Q7KZI7-3 Q7KZI7-4 Q7KZI7-5 Q7KZI7-6 Q7KZI7-7 Q7KZI7-8

Q7KZI7-9 Q7KZI7-10 Q7KZI7-11 Q7KZI7-12 Q7KZI7-13 Q7KZI7-14 Q7KZI7-15 Q7KZI7-16

(Produced by alternative splicing of isoform 1. Contains a phosphoserine at position 409)

Explore the universe of human proteins at neXtProt for MARK2: NX_Q7KZI7

Post-translational modifications:

Autophosphorylated. Phosphorylated at Thr-208 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo

kinase and CAB39. Phosphorylation at Thr-208 by TAOK1 activates the kinase activity, leading to phosphorylation and

detachment of MAPT/TAU from microtubules. Phosphorylation at Ser-212 by GSK3-beta (GSK3B) inhibits the kinase

activity. Phosphorylation by CaMK1 promotes activity and is required to promote neurite outgrowth. Phosphorylation at

Thr-596 by PRKCZ/aPKC in polarized epithelial cells inhibits the kinase activity and promotes binding to 14-3-3

protein YWHAZ, leading to relocation from cell membrane to cytoplasm¹

View modification sites using PhosphoSitePlus²

View neXtProt modification sites for NX_Q7KZI7

4/23 DME Specific Peptides for MARK2 (Q7KZI7) (see all 23)

FRQIVSA

FAKVKLA

EYASGGE

LVAHGRM

MARK2 Protein expression data from MOPED and PaxDb: About this image

REFSEQ proteins (5 alternative transcripts):

NP_001034558.2 NP_001156768.1 NP_001156769.1 NP_004945.4 NP_059672.2

ENSEMBL proteins:

ENSP00000425765 ENSP00000355091 ENSP00000294247 ENSP00000423452 ENSP00000444956

ENSP00000444859 ENSP00000437509 ENSP00000423974 ENSP00000423844 ENSP00000421075

ENSP00000386128 ENSP00000415494 ENSP00000385751 ENSP00000326632 ENSP00000367040

ENSP00000389184 ENSP00000367041

Human Recombinant Protein Products for MARK2:

	EMD Millipore Purified and/or Recombinant MARK2 Protein
	Browse R&D Systems for human recombinant proteins
	Browse recombinant and purified proteins available from Enzo Life Sciences
	OriGene Purified Protein: MARK2 OriGene Protein Over-expression Lysate (see all 2): MARK2 OriGene Custom Protein Services for MARK2
	GenScript Custom Purified and Recombinant Proteins Services for MARK2
	Novus Biologicals MARK2 Proteins Novus Biologicals MARK2 Lysates
	Browse Sino Biological Recombinant Proteins
	Browse ProSpec Recombinant Proteins
	Uscn Proteins for MARK2

Gene Ontology (GO): 4 cellular component terms (GO ID links to tree view): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005634	nucleus	IEA	--
GO:0005886	plasma membrane	IEA	--
GO:0016020	membrane	IMP	14976552
GO:0045180	basal cortex	IEA	--

MARK2 for ontologies About GeneDecksing

MARK2 Antibody Products:

	EMD Millipore Mono- and Polyclonal Antibodies for the study of MARK2
	Browse R&D Systems for Antibodies
	Cell Signaling Technology (CST) Antibodies for MARK2
	OriGene Antibodies (see all 2): MARK2 OriGene Custom Antibody Services for MARK2
	GenScript Custom Superior Antibodies Services for MARK2
	Novus Biologicals MARK2 Antibodies
	Abcam antibodies for MARK2
	Uscn Antibodies for MARK2
	ThermoFisher Antibodies for MARK2

Assay Products for MARK2:

	Browse Kits and Assays available from EMD Millipore
	OriGene Custom Immunoassay Development Browse OriGene Fluorogenic Cell Assay Kits
	Browse R&D Systems for biochemical assays
	GenScript Custom Assay Services for MARK2
	Browse Enzo Life Sciences for kits & assays
	Uscn ELISAs and CLIAs for MARK2

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

MARK2 for domains About GeneDecksing

5/7 InterPro domains/families (see all 7):

IPR017441 Protein_kinase_ATP_BS

IPR001772 KA1_dom

IPR002290 Ser/Thr_dual-sp_kinase_dom

IPR011009 Kinase-like_dom

IPR008271 Ser/Thr_kinase_AS

Graphical View of Domain Structure for InterPro Entry Q7KZI7

ProtoNet protein and cluster: Q7KZI7

2 Blocks protein families:

IPB000449 Ubiquitin-associated domain

IPB001772 Kinase-associated

UniProtKB/Swiss-Prot: MARK2_HUMAN, Q7KZI7

Domain: The UBA domain does not seem to bind ubiquitin and ubiquitin-like and might play a role in regulating the

enzyme conformation and localization. Activation of the kinase activity following phosphorylation at Thr-208 is

accompanied by a conformational change that alters the orientation of the UBA domain with respect to the catalytic

domain

Domain: The KA1 domain mediates binding to phospholipids and targeting to membranes (By similarity)

Similarity: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily

Similarity: Contains 1 KA1 (kinase-associated) domain

Similarity: Contains 1 protein kinase domain

Similarity: Contains 1 UBA domain

(According to ¹UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or ²DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory,
bound targets from SABiosciences, miRNA Gene Targets from miRTarBase, shRNA from OriGene, Sirion Biotech, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Sirion Biotech, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, Sirion Biotech, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
About This Section

Molecular Function:

UniProtKB/Swiss-Prot Summary: MARK2_HUMAN, Q7KZI7