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MAPKSP1 Gene

protein-coding   GIFtS: 53

GC04M101023
MAPK scaffold protein 1
(Previous name: mitogen-activated protein kinase kinase 1 interacting protein 1 )
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
(Previous symbol: MAP2K1IP1)
Services    

Aliases &
Descriptions
for MAPKSP1

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section
Aliases
MAP2K1IP1 2, 3
MAPBP 1, 2
MP1 1, 2
Mp1 3
PRO0633 2
Descriptions
MAPK scaffold protein 1 2
MEK binding partner 1 2
MEK partner 1 2
MEK-binding partner 1 3
Mitogen-activated protein kinase kinase 1-interacting
protein 1 3
mitogen-activated protein kinase kinase 1 interacting
protein 1 1, 2
External Ids
HGNC: 156061
Entrez Gene: 86492
UniProtKB: Q9UHA43
Ensembl: ENSG000001092707
Search outside databases for aliases for MAPKSP1 gene

Summaries
for MAPKSP1

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

 EntrezGene summary for MAPKSP1:
This gene encodes a scaffold protein that functions in the extracellular signal-regulated kinase
(ERK) cascade. The protein is localized to late endosomes by the mitogen-activated protein-binding
protein-interacting protein, and binds specifically to MAP kinase kinase MAP2K1/MEK1, MAP kinase
MAPK3/ERK1, and MAP kinase MAPK1/ERK2. Studies of the orthologous gene in mouse indicate that it
regulates late endosomal traffic and cell proliferation. Multiple transcript variants are
expressed by this gene, but only one variant is thought to express a protein. [provided by RefSeq]

UniProtKB/Swiss-Prot: MPKS1_HUMAN, Q9UHA4
Function: Adapter protein that enhances the efficiency of the MAP kinase cascade. Facilitates the
activation of MAPK2 (By similarity)

Gene Wiki entry for MAPKSP1 (MAP2K1IP1)

Genomic Location
for MAPKSP1

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

 Genomic View:
UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 4q23   Ensembl cytogenetic band:  4q23   HGNC cytogenetic band: 4q24-q26

MAPKSP1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 4         GeneLoc Exon Structure

GeneLoc location for GC04M101023:     (about GC identifiers)

Start:
 101,021,387 bp from pter
End:
 101,034,726 bp from pter
Size:
 13,340 bases
Orientation:
 minus strand
RefSeq DNA sequence:
NC_000004.10  NT_016354.18  

Proteins
for MAPKSP1

(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

 UniProtKB/Swiss-Prot: MPKS1_HUMAN, Q9UHA4 (See protein sequence)
Recommended Name: Mitogen-activated protein kinase scaffold protein 1  
Size: 124 amino acids; 13623 Da
Subunit: Interacts with MAP2K1 and MAPK2. Interacts with MORG1 (By similarity). Heterodimer with
MAPBPIP
Subcellular location: Late endosome membrane; Peripheral membrane protein; Cytoplasmic side (By
similarity)
PDB structures from and Proteopedia :
1SKO (3D)    2ZL1 (3D)    3CPT (3D)    
Secondary accessions: B2R4A1 Q9H364

REFSEQ proteins: NP_068805.1  

ENSEMBL proteins: 
ENSP00000226522 


Human Recombinant Proteins 
Browse Drug Discovery Central at Invitrogen for human recombinant proteins
Browse Purified and Recombinant Proteins at Millipore
Browse Human Recombinant Proteins at Sigma-Aldrich  
Browse R&D Systems for human recombinant proteins
Browse recombinant and purified proteins available from Enzo Life Sciences
Recombinant Proteins from Abcam (MAP2K1IP1)
Human Recombinant Proteins from Abnova (MAP2K1IP1)
                Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

2 Gene Ontology (GO) cellular component terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0016020 membrane IEA--
GO:0031902 late endosome membrane IEA--
About this table

Antibodies for MAPKSP1: 
Browse Antibodies Central at Invitrogen
Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
Sigma-Aldrich Antibodies for MAPKSP1
R&D Systems Antibodies for MAPKSP1 (MP1)
Antibodies from Abcam (MAP2K1IP1), each with their AbpromiseSM
Monoclonal and Polyclonal Antibodies from Abnova (MAP2K1IP1)
Novus Biologicals Antibodies for MAPKSP1

Assays for MAPKSP1: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

Protein Domains/
Families
for MAPKSP1

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section
1 InterPro domain/family:
 IPR015019 MAPKK1_Int


   GeneDecks  MAPKSP1 for the domains selected above  
About GeneDecksing

Graphical View of Domain Structure for InterPro Entry Q9UHA4

ProtoNet protein and cluster: Q9UHA4

UniProtKB/Swiss-Prot: MPKS1_HUMAN, Q9UHA4
Similarity: Belongs to the MAPKSP1 family

Gene Function
for MAPKSP1

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section
Inhib.
RNA:		Invitrogen RNAi Products for gene knock-down (MAPKSP1)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (MAP2K1IP1)
               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_021970

              Applied Biosystems Silencer® siRNAs for MAPKSP1

              Sigma-Aldrich siRNA for MAPKSP1  
                     Sigma-Aldrich shRNA for MAPKSP1  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Invitrogen Clones for MAPKSP1
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_021970
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_021970
                                 untagged cDNA clone in CMV expression vector: NM_021970 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_021970

UniProtKB/Swiss-Prot: MPKS1_HUMAN, Q9UHA4
Function: Adapter protein that enhances the efficiency of the MAP kinase cascade. Facilitates the
activation of MAPK2 (By similarity)

2 Gene Ontology (GO) molecular function terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005515 protein binding IPI15016825
GO:0019209 kinase activator activity IEA--
About this table

Pathways & Interactions
for MAPKSP1

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section


4 Sigma-Aldrich "Your Favorite Gene" Pathways for  MAPKSP1  (Your Favorite Gene powered by Ingenuity) 
 Molecular Mechanisms of Cancer
 ERK/MAPK Signaling
 G-Protein Coupled Receptor Signaling
 cAMP-mediated Signaling

   GeneDecks  MAPKSP1 for the pathways selected above  
About GeneDecksing

1 Cell Signaling Technology (CST) Pathway for MAPKSP1: 
 MAPK/Erk in Growth and Differentiation

   GeneDecks  MAPKSP1 for the pathways selected above  
About GeneDecksing

1 Kegg Pathway  (Kegg details for MAPKSP1):
 hsa04010 MAPK signaling pathway

   GeneDecks  MAPKSP1 for the pathways selected above  
About GeneDecksing
 Gene Network CentralTM Interacting Genes and Proteins Network for  MAPKSP1 


5/6 Interacting proteins for MAPKSP1 (Q9UHA41 ENSP000002265223) via UniProtKB, MINT, and/or STRING (see all 6 )
InteractantInteraction Details
GeneCardExternal ID(s)
Robld3 Robld3 (xeno)Q9JHS31EBI-1038192, EBI-1038198
ROBLD3ENSP000003572853STRING (score=.996)
MAP2K1ENSP000003024863STRING (score=.981)
MAPK1ENSP000002158323STRING (score=.94)
MAP2K2ENSP000002629483STRING (score=.92)
About this table

1 Gene Ontology (GO) biological process term (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0000186 activation of MAPKK activity IEA--
About this table

Drugs & Compounds
for MAPKSP1

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for MAPKSP1

Transcripts
for MAPKSP1

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section
Inhib.
RNA:		Invitrogen RNAi Products for gene knock-down (MAPKSP1)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (MAP2K1IP1)
               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_021970

              Sigma-Aldrich siRNA for MAPKSP1  
                     Sigma-Aldrich shRNA for MAPKSP1  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_021970  

REFSEQ mRNAs for MAPKSP1 gene: 

NM_021970.3   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_021970  

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_021970
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_021970
                                 untagged cDNA clone in CMV expression vector: NM_021970 

Additional cDNA sequence: 

AF130115.1 AF201947.1 AF305816.1 AK022313.1 AK093581.1 AK223311.1 AK311755.1 BC005340.1 
BC026245.1 BC110402.1 CR598975.1 CR602830.1 CR611511.1 CR613681.1 

8 DOTS entries:

DT.453163  DT.100811747  DT.100688450  DT.95230755  DT.40198023  DT.100811746  DT.95156954  DT.100739854 

24/377 AceView cDNA sequences (see all 377 ):

Z40283 BM141827 BI964883 BM141726 AU121987 AI418822 AA316206 BM769857 
BM141680 CR611511 AI147357 AW043674 F04907 BM141938 BF475586 AI027406 
AI332604 CK819906 BM830792 BM474916 BQ932027 BU674511 AI761245 AA854295 

Unigene Cluster for MAPKSP1:

MAPK scaffold protein 1
Hs.716375  [show with all ESTs]
Unigene Representative Sequence: NM_021970


GeneLoc Exon Structure

2 Alternative Splicing Database (ASD) splice patterns (SP) for MAPKSP1

ExUns: 1a · 1b ^ 2 ^ 3 ^ 4 ^ 5a · 5b ^ 6 ^ 7a · 7b
SP1:                                                            
SP2:                                -                           

About this scheme

ECgene alternative splicing isoforms for MAPKSP1

1 Ensembl transcript including schematic representation:
ENST00000226522  

Expression
for MAPKSP1

(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

MAPKSP1 expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for MAPKSP1

1 / 2

7 probe-sets matching MAPKSP1 gene


Affymetrix
probe-set		 Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

46103_at2 U95-B 1 1.00 1.00 0.47 0.91 -- -- -- -- --

46221_i_at2 U95-B 1 1.00 1.00 0.75 1.20 -- -- -- -- --
46222_r_at2 U95-B 1 1.00 1.00 0.57 0.89 -- -- -- -- --

227562_at2 U133-B 1 1.00 1.00 -- -- -- -- -- -- --

217971_at2 U133-A 1 1.00 1.00 -- -- -- -- -- -- --

227562_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

217971_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
GeneDecks  MAPKSP1 for binary patterns associated with the probe-sets selected above  
About GeneDecksing
About this table    
Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: ATAGTACAGC

SOURCE GeneReport for Unigene cluster: Hs.716375

Orthologs
for MAPKSP1

(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section
Orthologs for MAPKSP1 gene from 5/11 species (see all 11 )
Organism Gene Locus Description Human
Similarity		 NCBI accessions
dog
(Canis familiaris)		 MAPKSP11   -- MAPK scaffold protein 1 94.89(n)
99.19(a)		 478492  XM_535670.2  XP_535670.1 
chimpanzee
(Pan troglodytes)		 MAPKSP11   -- MAPK scaffold protein 1 99.73(n)
100(a)		 461399  XM_001167664.1  XP_001167664.1 
cow
(Bos taurus)		 MAPKSP11   -- MAPK scaffold protein 1 93.55(n)
99.19(a)		 533155  NM_001075982.1  NP_001069450.1 
rat
(Rattus norvegicus)		 Map2k1ip11   -- mitogen-activated protein kinase kinase 1 interacting more 88.71(n)
96.77(a)		 362045  NM_001008375.1  NP_001008376.1 
mouse
(Mus musculus)		 Mapksp15 3 MAPK scaffold protein 1   -- BG075477 
About this table        Species with no ortholog for MAPKSP1

ENSEMBL Gene Tree for MAPKSP1

Paralogs
for MAPKSP1

(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

Pseudogenes
2 related pseudogenes


SNPs/Variants
for MAPKSP1

(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section


10/43 NCBI SNPs in MAPKSP1 are shown (see all 43 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 23)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 4 posSequenceRecsAA
Chg		TypeMoreRecsAllele
freq		PopTotal
sample		More
------------
rs119444051,2
C,F,H101018984(+) AGCACT/CATTAA 1 -- ut314Minor allele frequency- C:0.28EU EA WA 418
rs22987351,2
C,F,H101034640(-) GCGGCG/TTAGAA 1 -- ut515Minor allele frequency- T:0.50EA EU WA 1896
rs22987341,2
F,H101035123(-) CTCCCC/TATTCA 1 -- ng31 tfbs35Minor allele frequency- T:0.25EA EU WA 1916
rs65328351,2
A,C,F,H101024230(+) AAGGTC/AATTAA 1 -- int13Minor allele frequency- A:0.40EU EA 246
rs13654941,2
C,F,H,O101025275(+) ACTAAC/TTTCTT 1 -- int1 trp316Minor allele frequency- N:0.00EA NA EU WA 1416
rs76882841,2
C,F,H101033203(+) AAGTGT/CTTAAC 1 -- int14Minor allele frequency- C:0.03EU EA WA 416
rs119453031,2
C,F,H101029634(+) ctctaT/Cgtacc 1 -- int14Minor allele frequency- C:0.28EU EA WA 418
rs119379851,2
C,F,H101027932(+) TGATAC/TTATGA 1 -- int112Minor allele frequency- T:0.27EA NA EU WA 1054
rs100167411,2
C,F,H101026244(+) GTATCT/GCTCAT 1 -- int18Minor allele frequency- G:0.16EU EA WA 828
rs68483371,2
A,C101030219(+) AATATA/GAATAG 1 -- int10--------
About this table

HapMap Linkage Disequilibrium images for MAPKSP1 (up to first 250kb)

Disorders & Mutations
for MAPKSP1

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

 OMIM: 603296

Medical News
for MAPKSP1

(Possibly Related Articles in Doctor's Guide)
About This Section

   --

Publications
for MAPKSP1

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
About This Section

10/23 PubMed articles for MAPKSP1 gene (see all 23 ):

Search for MAPKSP1

(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

 ANDOR
Aliases
Free Text  
  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)

Genome Databases showing MAPKSP1

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section
Entrez Gene: 8649 HGNC: 15606 AceView: MAP2K1IP1 Ensembl:ENSG00000109270 euGenes: HUgn8649
ECgene: MAPKSP1 H-InvDB: MAPKSP1

Other Databases showing MAPKSP1

(According to HUGE)
About This Section

   --

Specialized Databases showing MAPKSP1

(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

   --

Licensable Technologies
for MAPKSP1

(Available from WIS Yeda, Salk, Tufts)
About This Section

  --

Services
for MAPKSP1

(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for MAPKSP1:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



  Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
  Invitrogen Antibodies  Invitrogen Assays
  Invitrogen Clones  Invitrogen Q-PCR Products
  Invitrogen Human Recombinant Kinases  Invitrogen Custom Antibody and Peptide Service
  Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for MAPKSP1-related products

 Millipore Custom Antibody & Bulk Services
 Millipore Preclinical / Clinical Development Services
 Millipore Immunoassay Services
 Millipore Target Screening & Profiling Services


 Predesigned and custom siRNAs for MAPKSP1 Antibodies for MAPKSP1
 Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
 Lentivirus-delivered shRNAs for MAPKSP1 Browse small molecules at Sigma-Aldrich
 "Your Favorite Gene" Pathwaysfeedback


  
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 Recombinant & Natural Proteins  

 Recombinant Proteins (MAP2K1IP1)
 Antibodies (MAP2K1IP1)
 Chimera RNAi (MAP2K1IP1)
 Custom Service for Mouse Mab
 Custom Service for Rabbit Pab from Full-length Protein
  
 Search for Antibodies & Assays

 Recombinant Proteins
(MAP2K1IP1)
 Antibodies (MAP2K1IP1)
 Search OriGene for MAPKSP1
 Search Tocris compounds for MAPKSP1




 Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
 Antibodies for MAPKSP1

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