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Category Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21

GIFtS
-

MAP3K8 Gene

protein-coding GIFtS: 68
GCID: GC10P030722

mitogen-activated protein kinase kinase kinase 8

(Previous symbols: COT, ESTF)

Explore 14 diseases affiliated with
MAP3K8 via

MalaCards

our new
Human Malady Compendium

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc, ⁷Ensembl, ⁸DME, ⁹miRBase, and/or ¹⁰fRNAdb)
About This Section

Aliases
Mitogen-Activated Protein Kinase Kinase Kinase 8¹ ²		TPL2² ⁵
COT¹ ² ³ ⁵		Cot (Cancer Osaka Thyroid) Oncogene²
ESTF¹ ² ³		Ewing Sarcoma Transformant²
EST¹ ² ⁵		Proto-Oncogene Serine/Threoine Protein Kinase²
MEKK8¹ ²		EC 2.7.11.25³
Tpl-2¹ ²		TPL-2³
C-COT¹		Cancer Osaka Thyroid Oncogene³
Proto-Oncogene C-Cot² ³		Serine/Threonine-Protein Kinase Cot³
Tumor Progression Locus 2² ³

External Ids:	HGNC: 6860¹	Entrez Gene: 1326²	Ensembl: ENSG00000107968⁷	OMIM: 191195⁵	UniProtKB: P41279³

Export aliases for MAP3K8 gene to outside databases

Previous GC identifers: GC10P030458 GC10P030873 GC10P030726 GC10P030762

(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for MAP3K8:

This gene is an oncogene that encodes a member of the serine/threonine protein kinase family. The encoded protein

localizes to the cytoplasm and can activate both the MAP kinase and JNK kinase pathways. This protein was shown to

activate IkappaB kinases, and thus induce the nuclear production of NF-kappaB. This protein was also found to promote

the production of TNF-alpha and IL-2 during T lymphocyte activation. This gene may also utilize a downstream in-frame

translation start codon, and thus produce an isoform containing a shorter N-terminus. The shorter isoform has been

shown to display weaker transforming activity. Alternate splicing results in multiple transcript variants that encode

the same protein. (provided by RefSeq, Sep 2011)

UniProtKB/Swiss-Prot: M3K8_HUMAN, P41279

Function: Required for TLR4 activation of the MEK/ERK pathway. Able to activate NF-kappa-B 1 by stimulating

proteasome-mediated proteolysis of NF-kappa-B 1/p105. Plays a role in the cell cycle. The longer form has some

transforming activity, although it is much weaker than the activated cot oncoprotein

Gene Wiki entry for MAP3K8

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section

RefSeq DNA sequence:

NC_000010.10 NC_018921.1 NT_008705.16

Regulatory elements:

SABiosciences Regulatory transcription factor binding sites in the MAP3K8 gene promoter:
Pbx1a AML1a Nkx2-5 Nkx5-1 CREB MEF-2A deltaCREB aMEF-2 NF-kappaB1
Other transcription factors

Browse SwitchGear Promoter luciferase reporter plasmids

Search SABiosciences Chromatin IP Primers for MAP3K8

Epigenetics:

QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat MAP3K8

Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 10p11.23 Ensembl cytogenetic band: 10p11.23 HGNC cytogenetic band: 10p11.2

MAP3K8 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
MAP3K8 gene location

GeneLoc information about chromosome 10 GeneLoc Exon Structure

GeneLoc location for GC10P030722: view genomic region (about GC identifiers)

Start:	30,722,866 bp from pter	End:	30,750,762 bp from pter
Size:	27,897 bases	Orientation:	plus strand

(According to ¹UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to ²PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: M3K8_HUMAN, P41279 (See protein sequence)

Recommended Name: Mitogen-activated protein kinase kinase kinase 8

Size: 467 amino acids; 52925 Da

Cofactor: Magnesium

Subunit: Forms a ternary complex with NFKB1 and TNIP2

Subcellular location: Cytoplasm

Developmental stage: Isoform 1 is activated specifically during the S and G2/M phases of the cell cycle

Secondary accessions: A8K2Q5 D3DRX1 Q14275 Q5T855 Q9HC81

Alternative initiation: 2 isoforms: P41279-1 P41279-2

Explore the universe of human proteins at neXtProt for MAP3K8: NX_P41279

Post-translational modifications:

Autophosphorylated. Isoform 1 undergoes phosphorylation mainly on Ser residues, and isoform 2 on both Ser and Thr

residues¹

View modification sites using PhosphoSitePlus²

View neXtProt modification sites for NX_P41279

MAP3K8 Protein expression data from MOPED and PaxDb: About this image

REFSEQ proteins (2 alternative transcripts):

NP_001231063.1 NP_005195.2

ENSEMBL proteins:

ENSP00000364477 ENSP00000409653 ENSP00000364471 ENSP00000391275 ENSP00000364470

ENSP00000411580 ENSP00000263056 ENSP00000443610

Reactome Protein details: P41279
Human Recombinant Protein Products:

	EMD Millipore Purified and/or Recombinant MAP3K8 Protein
	R&D Systems Recombinant & Natural Proteins for MAP3K8 (MAP3K8/Tpl2/COT)
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	Novus Biologicals MAP3K8 Proteins Novus Biologicals MAP3K8 Lysate
	Browse Sino Biological Recombinant Proteins
	Browse ProSpec Recombinant Proteins
	Uscn Proteins for MAP3K8

Gene Ontology (GO): 2 cellular component terms (GO ID links to tree view): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005737	cytoplasm	IDA	--
GO:0005829	cytosol	TAS	--

MAP3K8 for ontologies About GeneDecksing

MAP3K8 Antibody Products:

	Browse EMD Millipore's Extensive Line of Mono- and Polyclonal Antibodies
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	GenScript Custom Superior Antibodies Services for MAP3K8
	Novus Biologicals MAP3K8 Antibodies
	Abcam antibodies for MAP3K8
	Uscn Antibodies for MAP3K8
	ThermoFisher Antibody for MAP3K8

Assay Products for MAP3K8:

	Browse Kits and Assays available from EMD Millipore
	OriGene Custom Immunoassay Development Browse OriGene Fluorogenic Cell Assay Kits
	Browse R&D Systems for biochemical assays
	GenScript Custom Assay Services for MAP3K8
	Browse Enzo Life Sciences for kits & assays
	Uscn ELISAs and CLIAs for MAP3K8

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

MAP3K8 for domains About GeneDecksing

4 InterPro domains/families:

IPR011009 Kinase-like_dom

IPR008271 Ser/Thr_kinase_AS

IPR000719 Prot_kinase_cat_dom

IPR017424 MAPKKK8

Graphical View of Domain Structure for InterPro Entry P41279

ProtoNet protein and cluster: P41279

UniProtKB/Swiss-Prot: M3K8_HUMAN, P41279

Similarity: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase

subfamily

Similarity: Contains 1 protein kinase domain

(According to ¹UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or ²DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
About This Section

Function Summary:

UniProtKB/Swiss-Prot: M3K8_HUMAN, P41279

Function: Required for TLR4 activation of the MEK/ERK pathway. Able to activate NF-kappa-B 1 by stimulating

proteasome-mediated proteolysis of NF-kappa-B 1/p105. Plays a role in the cell cycle. The longer form has some

transforming activity, although it is much weaker than the activated cot oncoprotein

Catalytic activity: ATP + a protein = ADP + a phosphoprotein

Genatlas biochemistry entry for MAP3K8:

mitogen-activated protein kinase kinase kinase 8,with two alternatively spliced transcripts

Enzyme Number (IUBMB): EC 2.7.11.25¹

miRNA Products:		miRTarBase miRNAs that target MAP3K8: hsa-mir-370 (MIRT001074)

		OriGene 3'-UTR Clone: MAP3K8 Browse MicroRNA Expression Plasmids
		QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat MAP3K8
		8/24 QIAGEN miScript miRNA Assays for microRNAs that regulate MAP3K8 (see all 24):
		hsa-miR-4328 hsa-miR-539 hsa-miR-106a hsa-miR-519a hsa-miR-105 hsa-miR-144 hsa-miR-501-5p hsa-miR-93

		SwitchGear 3'UTR luciferase reporter plasmid: MAP3K8 3' UTR sequence

Inhib. RNA Products:		Browse for Gene Knock-down Tools from EMD Millipore
		OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for MAP3K8 (see all 7) OriGene shRNA RFP: MAP3K8 OriGene siRNA: MAP3K8
		QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat MAP3K8

Gene Editing
Products:

DNA2.0 Custom Protein Engineering Service for MAP3K8

Clone Products:		Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore
		OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for MAP3K8 (see all 3) OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for MAP3K8 (see all 2) OriGene custom cloning services � gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
		GenScript: all cDNA clones in your preferred vector: MAP3K8 (NM_005204)
		Sino Biological Human cDNA Clone for MAP3K8
		DNA2.0 Custom Codon Optimized Gene Synthesis Service for MAP3K8
		Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat MAP3K8

Cell Line Products:		GenScript Custom overexpressing Cell Line Services for MAP3K8
		LifeMap BioReagents: cell line associated with MAP3K8: PureStem E15, Meso-prx/latp Progenitor

In Situ Assay
Products:

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for MAP3K8

Gene Ontology (GO): 5/6 molecular function terms (GO ID links to tree view) (see all 6): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0000287	magnesium ion binding	IEA	--
GO:0004674	protein serine/threonine kinase activity	TAS	8226782
GO:0004709	MAP kinase kinase kinase activity	IEA	--
GO:0005515	protein binding	IPI	--
GO:0005524	ATP binding	IEA	--

MAP3K8 for ontologies About GeneDecksing

5 GenomeRNAi human phenotypes for MAP3K8:

Decreased focal adhesion (FA)	Decreased substrate adherent c	Downregulation of NF-kappaB pa	Increased cell death HMECs cel
Wnt reporter downregulated

Animal Models:
Mouse knock-outs for MAP3K8: Map3k8^tm1Tmat Map3k8^tm1Ceci
5 MGI mutant phenotypes (inferred from 3 alleles) (MGI details for Map3k8):

cellular

digestive/alimentary

hematopoietic system

immune system

mortality/aging

MAP3K8 for phenotypes About GeneDecksing

(Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to ¹UniProtKB, ²MINT, ³I2D, and/or ⁴STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
About This Section

Unified GeneCards pathways - 5/24 super-pathways (see all 24) About this table

See pathways by source

Super-pathway

contained gene-specific pathways

CD28 co-stimulation

	CD28 co-stimulation	1.00			Costimulation by the CD28 family	0.41
	CD28 dependent PI3K/Akt signaling	0.66

Signal transduction_JNK pathway

Signal transduction_JNK pathway

1.00

Signal transduction JNK pathway

0.98

Immune System

Immune System

1.00

Adaptive Immune System

0.59

Toll-like receptor signaling pathway

Toll-like receptor signaling pathway

1.00

Toll-like receptor signaling pathway

0.97

MAPK signaling pathway

MAPK signaling pathway

1.00

MAPK signaling pathway

0.50

Pathway sources
See GeneCards unified pathways
Show all pathways

1 EMD Millipore Pathway for MAP3K8

Signal transduction JNK pathway

5/8 Downloadable PowerPoint Slides of QIAGEN Pathway Central Maps for MAP3K8 (see all 8)

	MAPK Signaling
	TNFR2 Pathway
	ERK5 Signaling
	NFAT and Cardiac Hypertrophy
	MAPK Family Pathway

1 Cell Signaling Technology (CST) Pathway for MAP3K8

MAP Kinase Signaling

1 GeneGo (Thomson Reuters) Pathway for MAP3K8

Signal transduction JNK pathway

5/10 BioSystems Pathways for MAP3K8 (see all 10)

	MAPK signaling pathway
	Toll-like receptor signaling pathway
	TCR Signaling Pathway
	Insulin Signaling
	TNF-alpha/NF-kB Signaling Pathway

5/8 Reactome Pathways for MAP3K8 (see all 8)

	Costimulation by the CD28 family
	Cytokine Signaling in Immune system
	Adaptive Immune System
	CD28 dependent PI3K/Akt signaling
	Interleukin-1 signaling

3 Kegg Pathways (Kegg details for MAP3K8):

	MAPK signaling pathway
	Toll-like receptor signaling pathway
	T cell receptor signaling pathway

MAP3K8 for pathways About GeneDecksing

Interactions:

SABiosciences Gene Network Central^TM Interacting Genes and Proteins Network for MAP3K8

STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

5/60 Interacting proteins for MAP3K8 (P41279^{1, 2, 3} ENSP00000263056⁴) via UniProtKB, MINT, STRING, and/or I2D (see all 60)

Interactant		Interaction Details
GeneCard	External ID(s)	Interaction Details
NFKB2	Q00653²^,³, ENSP00000189444⁴	MINT-47914 MINT-47828 MINT-47833 I2D: score=5 STRING: ENSP00000189444
NFKB1	P19838²^,³, ENSP00000226574⁴	MINT-47827 I2D: score=6 STRING: ENSP00000226574
TNIP2	Q8NFZ5¹^,²^,³, ENSP00000321203⁴	EBI-354900,EBI-359372 MINT-47826 I2D: score=7 STRING: ENSP00000321203
RELA	Q04206²^,³, ENSP00000384273⁴	MINT-47829 MINT-47834 I2D: score=3 STRING: ENSP00000384273
CALM1	P62158³, ENSP00000349467⁴	I2D: score=1 STRING: ENSP00000349467

About this table

Gene Ontology (GO): 3 biological process terms (GO ID links to tree view): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0006468	protein phosphorylation	TAS	2072910
GO:0007049	cell cycle	IEA	--
GO:0031295	T cell costimulation	TAS	--

MAP3K8 for ontologies About GeneDecksing

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
About This Section

MAP3K8 for compounds About GeneDecksing

EMD Millipore small molecules for MAP3K8:
Small Molecule - inhibitor

Browse drugs & compounds from Enzo Life Sciences

Browse Tocris compounds for MAP3K8

3 HMDB Compounds for MAP3K8 About this table

Compound	Synonyms	CAS #	PubMed Ids
ADP	adenosindiphosphorsaeure (see all 8)	58-64-0	--
Adenosine triphosphate	5'-(tetrahydrogen triphosphate) Adenosine (see all 24)	56-65-5	--
Magnesium	Magnesium (see all 2)	7439-95-4	--

2 Novoseek chemical compound relationships for MAP3K8 gene About this table

Compound	-log (P-Val)	Hits	PubMed IDs for Articles with Shared Sentences (# sentences)
serine	50	3	2072910 (1), 19808894 (1), 10669751 (1)
threonine	49.2	1	10669751 (1)

Search CenterWatch for drugs/clinical trials and news about MAP3K8 / M3K8

(Secondary structures according to fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from EMD Millipore,
siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
About This Section

REFSEQ mRNAs for MAP3K8 gene (2 alternative transcripts):

NM_001244134.1 NM_005204.3

Unigene Cluster for MAP3K8:

Mitogen-activated protein kinase kinase kinase 8

Hs.432453 [show with all ESTs]

Unigene Representative Sequence: NM_005204

8 Ensembl transcripts including schematic representations, and UCSC links where relevant:

ENST00000375328(uc009xlf.2) ENST00000415139 ENST00000375322 ENST00000413724

ENST00000375321(uc001ivj.2) ENST00000430603 ENST00000263056(uc001ivi.2)

ENST00000542547

miRNA Products:		OriGene 3'-UTR Clone: MAP3K8 Browse OriGene MicroRNA Expression Plasmids
		QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat MAP3K8
		8/24 QIAGEN miScript miRNA Assays for microRNAs that regulate MAP3K8 (see all 24):
		hsa-miR-4328 hsa-miR-539 hsa-miR-106a hsa-miR-519a hsa-miR-105 hsa-miR-144 hsa-miR-501-5p hsa-miR-93

		SwitchGear 3'UTR luciferase reporter plasmid: MAP3K8 3' UTR sequence

Inhib. RNA Products:		Browse for Gene Knock-down Tools from EMD Millipore
		OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for MAP3K8 (see all 7) OriGene shRNA RFP: MAP3K8 OriGene siRNA: MAP3K8
		QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat MAP3K8

Clone Products:		OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for MAP3K8 (see all 3) OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for MAP3K8 (see all 2) OriGene custom cloning services � gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
		GenScript: all cDNA clones in your preferred vector: MAP3K8 (NM_005204)
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		Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat MAP3K8

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		SABiosciences RT² qPCR Primer Assay in human, mouse, rat MAP3K8
		QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat MAP3K8
		QIAGEN QuantiFast Probe-based Assays in human, mouse, rat MAP3K8

Additional cDNA sequence:

AK290320.1 AK313295.1 BC104833.1 BC113566.1 CR542284.1 D14497.1

4 DOTS entries:

DT.415680 DT.100773621 DT.92424295 DT.415684

24/122 AceView cDNA sequences (see all 122):

BM848442 AI042009 BI753824 CF994198 AA156840 CB124240 CB125233 AI362092

AA677028 CB125814 AI471552 BX103131 BI908729 CD365718 AV659361 CR619928

CF594092 CD367347 CA426041 BU753921 BU929720 NM_005204 T29129 BU934131

GeneLoc Exon Structure

5/7 Alternative Splicing Database (ASD) splice patterns (SP) for MAP3K8 (see all 7) About this scheme

ExUns:	1a	·	1b	·	1c	^	2a	·	2b	^	3a	·	3b	·	3c	^	4a	·	4b	·	4c	·	4d	^	5	^	6a	·	6b	·	6c	^	7	^	8	^	9	^	10
SP1:							-		-		-				-		-
SP2:
SP3:							-		-		-		-		-		-
SP4:											-		-		-		-
SP5:											-				-		-

ECgene alternative splicing isoforms for MAP3K8

(RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section

MAP3K8 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: AATCTTTCAT

About this image

MAP3K8 expression in embryonic tissues and stem cells

Expression by the Database of Embryonic development, Stem cell research, and Regenerative medicine About this table

Stem Cell Differentiation: 1 LifeMap Cell

Name

Category

PureStem™ mesenchymal progenitor E15 (Embryonic Progenitor Cell)

Adipose, Bone, Cartilage

Expression:

Positive

Negative

Selective marker

Experimental details:

Curated

Microarrays

In-situ hybridization

See MAP3K8 Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for MAP3K8

SOURCE GeneReport for Unigene cluster: Hs.432453

UniProtKB/Swiss-Prot: M3K8_HUMAN, P41279

Tissue specificity: Expressed in several normal tissues and human tumor-derived cell lines

SABiosciences Custom PCR Arrays for MAP3K8

Primer Products:		OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for MAP3K8 Browse OriGene validated miRNA SYBR primer pairs
		SABiosciences RT² qPCR Primer Assay in human, mouse, rat MAP3K8
		QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat MAP3K8
		QIAGEN QuantiFast Probe-based Assays in human, mouse, rat MAP3K8

In Situ
Assay Products:

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for MAP3K8

(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD , ⁵MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or ⁶Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
About This Section

This gene was present in the common ancestor of chordates.

Orthologs for MAP3K8 gene from 4/13 species (see all 13) About this table

Organism	Taxonomic classification	Gene	Description	Human Similarity	Orthology Type	Details
mouse (Mus musculus)	Mammalia	Map3k8^{1 , 5}	mitogen-activated protein kinase kinase kinase 8^{1, 5}	87.79(n)¹ 93.36(a)¹		18 (2.73 cM)⁵ 26410¹ NM_007746.2¹ NP_031772.1¹ 4331327⁵
chicken (Gallus gallus)	Aves	MAP3K8¹	mitogen-activated protein kinase kinase kinase 8	76.18(n) 84.12(a)		420479 XM_418581.3 XP_418581.2
lizard (Anolis carolinensis)	Reptilia	MAP3K8⁶	--	81(a)	1 ↔ 1	6(13497525-13508788)
zebrafish (Danio rerio)	Actinopterygii	LOC563727¹	mitogen-activated protein kinase kinase kinase 8-like	62.32(n) 63.15(a)		563727 XM_687085.3 XP_692177.2

ENSEMBL Gene Tree for MAP3K8 (if available)
TreeFam Gene Tree for MAP3K8 (if available)

(Paralogs according to ¹HomoloGene,
²Ensembl, and ³SIMAP, Pseudogenes according to ⁴Pseudogene.org Build 68)
About This Section

Paralogs for MAP3K8 gene

MAP3K1² MAP3K3² MAP3K5² MAP3K15² MAP3K19² MAP3K2² MAP3K6²

2 SIMAP similar genes for MAP3K8 using alignment to 6 protein entries: M3K8_HUMAN (see all proteins):

PAK4 MST094

MAP3K8 for paralogs About GeneDecksing

(SNPs/Variants according to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
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SNP ID	Valid	Clinical significance	Chr 10 pos	Sequence	#	AA Chg	Type	More	#	Allele freq	Pop	Total sample	More
Genomic Data					Transcription Related Data				Allele Frequencies
				--	--	--		--	--
rs187757989^1,2		--	30720985(+)	`CGACGC/TTGGAT`	2	--	us2k¹		0	--	--	--	--
rs192890621^1,2		--	30720988(+)	`CGTTGG/TATTTG`	2	--	us2k¹		0	--	--	--	--
rs12763912^1,2	C,H	--	30721091(+)	`TACATC/ACTCGG`	2	--	us2k¹		4		NS EA	416
rs117783631^1,2	F,	--	30721120(+)	`TTCCAT/CAGCAG`	2	--	us2k¹		1		EA	120
rs141205529^1,2		--	30721170(+)	`TTTTTA/TAAATT`	2	--	us2k¹		0	--	--	--	--
rs8176941^1,2	C,F,	--	30721195(+)	`CCGCGG/TTGGCT`	2	--	us2k¹		2		NS	92
rs8176942^1,2	C,F,	--	30721213(+)	`GTAATC/TCCAGC`	2	--	us2k¹		3		NS WA	92
rs12764530^1,2	H	--	30721504(+)	`ttgagA/Ccaagg`	2	--	us2k¹		4		NS EA	420
rs149633588^1,2		--	30721653(+)	`ATTTTC/TAGATT`	2	--	us2k¹		0	--	--	--	--
rs8176943^1,2	C,F,A,H,	--	30721805(+)	`GCACGG/AACCTG`	2	--	us2k¹		17		NS EA WA	2042

HapMap Linkage Disequilibrium report for MAP3K8 (30722866 - 30750762 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV): 1 variation for MAP3K8
1 CNV: 4674

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(in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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MAP3K8 for disorders           About GeneDecksing

OMIM gene information: 191195
OMIM disorders: 211980
14 diseases for MAP3K8:    About MalaCards

diffuse large b-cell lymphoma b-cell lymphomas sarcoma lung cancer, somatic

thyroiditis acute myeloid leukemia myeloid leukemia lung cancer

alzheimer's disease leukemia prostate cancer breast cancer

prostatitis carcinoma

3 Novoseek disease relationships for MAP3K8 gene About this table

Disease	-log (P-Val)	Hits	PubMed IDs for Articles with Shared Sentences (# sentences)
tumors	7.68	2	7937886 (1), 20045951 (1)
cancer	6.45	5	18831712 (1), 2072910 (1), 18319612 (1), 20045951 (1)
inflammation	0	2	15699325 (1), 16039990 (1)

Human Genome Epidemiology (HuGE) Navigator: MAP3K8 (4 documents)

Export disorders for MAP3K8 gene to outside databases

(in PubMed. Associations of this gene to articles via ¹Entrez Gene, ²UniProtKB/Swiss-Prot, ³HGNC, ⁴GAD, ⁵PharmGKB, ⁶HMDB, ⁷DrugBank, ⁸UniProtKB/TrEMBL, ⁹ Novoseek, and/or ¹⁰fRNAdb)
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PubMed articles for MAP3K8 gene, integrated from 9 sources (see all 92):
(articles sorted by number of sources associating them with MAP3K8)

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Structure and transforming potential of the human cot oncogene encoding a putative protein kinase. (PubMed id 2072910)^{1, 2, 3, 9} Miyoshi J.... Kakunaga T. (1991)
Expression cDNA cloning of a serine kinase transforming gene. (PubMed id 8479752)^{1, 2, 3} Chan A.M....Aaronson S.A. (1993)
ABIN-2 forms a ternary complex with TPL-2 and NF-kappa B1 p105 and is essential for TPL-2 protein stability. (PubMed id 15169888)^{1, 2, 9} Lang V....Ley S.C. (2004)
COT kinase proto-oncogene expression in T cells: implication of the JNK/SAPK signal transduction pathway in COT promoter activation. (PubMed id 10896655)^{1, 2, 9} Sanchez-Gongora E.... Alemany S. (2000)
The human cot proto-oncogene encodes two protein serine/threonine kinases with different transforming activities by alternative initiation of translation. (PubMed id 8226782)^{1, 2, 9} Aoki M.... Toyoshima K. (1993)
The DNA sequence and comparative analysis of human chromosome 10. (PubMed id 15164054)^{1, 2} Deloukas P.... Rogers J. (2004)
The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)^{1, 2} Gerhard D.S....Malek J. (2004)
Identification and characterization of protein products of the cot oncogene with serine kinase activity. (PubMed id 1833717)^{1, 2} Aoki M.... Toyoshima K. (1991)
The oncogenic protein kinase Tpl-2/Cot contributes to Epstein-Barr virus-encoded latent infection membrane protein 1-induced NF-kappaB signaling downstream of TRAF2. (PubMed id 11932422)^{1, 9} Eliopoulos A.G....Young L.S. (2002)
Proteomics analysis of immunoprecipitated proteins associated with the oncogenic kinase cot. (PubMed id 18319612)^{1, 9} Wu B. and Wilmouth R.C. (2008)

(in PubMed, OMIM, and NCBI Bookshelf)
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		AND	OR
Aliases
Disorders
Free Text

Query String		PubMed OMIM NCBI Bookshelf
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(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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Entrez Gene: 1326	HGNC: 6860	AceView: MAP3K8	Ensembl:ENSG00000107968	euGenes: HUgn1326
ECgene: MAP3K8	Kegg: 1326	H-InvDB: MAP3K8

(According to HUGE)
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(According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
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Name	Description
PharmGKB entry for MAP3K8	Pharmacogenomics, SNPs, Pathways
ATLAS Chromosomes in Cancer entry for MAP3K8	Genetics and Cytogenetics in Oncology and Haematology
SeattleSNPs	http://pga.gs.washington.edu/data/map3k8/

Intellectual Property for MAP3K8 gene
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Patent Information for MAP3K8 gene:
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mitogen-activated protein kinase kinase kinase 8

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mitogen-activated protein kinase kinase kinase 8

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