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MAK10 Gene

protein-coding GIFtS: 43

GC09P087745

MAK10 homolog, amino-acid N-acetyltransferase subunit (S. cerevisiae)
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc , and/or ⁷Ensembl, ⁸miRBase)
About This Section

Aliases
EGAP ^³
FLJ21613 ^¹, ²
FLJ22643 ^¹, ²
OTTHUMP00000021584 ^²
bA379P1.1 ^¹, ²

Descriptions
Embryonic growth-associated protein homolog ^³
MAK10 homolog, amino-acid N-acetyltransferase subunit (S. cerevisiae) ^²
corneal wound healing-related protein ^²

External Ids
HGNC: 24340¹
Entrez Gene: 60560²
UniProtKB: Q5VZE5³
Ensembl: ENSG00000135040⁷

Search outside databases for aliases for MAK10 gene

Previous GC identifers: GC09P084014 GC09P085785

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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UniProtKB/Swiss-Prot: MAK10_HUMAN, Q5VZE5

Function: Component of NAT13-MAK10 complex, which probably displays alpha (N-terminal)

acetyltransferase activity. Regulates proliferation of smooth muscle cells (By similarity)

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:

UCSC Golden Path with GeneCards custom track

Transcription factor binding sites upstream to the MAK10 gene

Entrez Gene cytogenetic band: 9q21.33 Ensembl cytogenetic band: 9q21.33 HGNC cytogenetic band: 9q22.1

MAK10 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 9 GeneLoc Exon Structure

GeneLoc location for GC09P087745: (about GC identifiers)

Start:	87,745,877 bp from pter
End:	87,827,037 bp from pter
Size:	81,161 bases
Orientation:	plus strand

RefSeq DNA sequence:

NC_000009.10 NT_023935.17

(According to ¹UniProtKB, and/or Ensembl, Phosphorylation sites according to ²Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: MAK10_HUMAN, Q5VZE5 (See protein sequence)

Recommended Name: Protein MAK10 homolog

Size: 725 amino acids; 83639 Da

Subunit: Interacts with NAT13 (By similarity)

Subcellular location: Cytoplasm (By similarity)

Sequence caution: Sequence=BAB15097.1; Type=Frameshift; Positions=518;

Secondary accessions: Q9H631 Q9H703

REFSEQ proteins: NP_078911.3

ENSEMBL proteins:

ENSP00000365209 ENSP00000365208 ENSP00000354972

Human Recombinant Proteins

	Browse Drug Discovery Central at Invitrogen for human recombinant proteins
	Browse Purified and Recombinant Proteins at Millipore
	Browse Human Recombinant Proteins at Sigma-Aldrich
	Browse R&D Systems for human recombinant proteins
	Browse recombinant and purified proteins available from Enzo Life Sciences
	Browse Abcam for Recombinant Proteins
	Browse Abnova for Human Recombinant Proteins

Browse Origene for full length recombinant human proteins expressed in human HEK293 cells

1 Gene Ontology (GO) cellular component term (links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005737	cytoplasm	ISS	--

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Antibodies for MAK10:

	Browse Antibodies Central at Invitrogen
	Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
	Sigma-Aldrich Antibodies for MAK10
	Browse R&D Systems for Antibodies
	Search for antibodies at Abcam and the World's Antibody Gateway (free search engine of over 150 antibody companies)
	Browse Abnova for Monoclonal and Polyclonal Antibodies
	Search Novus for antibodies for MAK10

Assays for MAK10:

	Browse Invitrogen for biochemical assays
	Browse Kits and Assays available from Millipore
	Browse R&D Systems for biochemical assays
	Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

Graphical View of Domain Structure for InterPro Entry Q5VZE5

ProtoNet protein and cluster: Q5VZE5

UniProtKB/Swiss-Prot: MAK10_HUMAN, Q5VZE5

Similarity: Belongs to the MAK10 family

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

Inhib. RNA:		Invitrogen RNAi Products for gene knock-down (MAK10)
		Browse for Gene Knock-down Tools from Millipore
		Browse Abnova for Chimera RNAi Products

OriGene 29mer shRNA kit in GFP-retroviral vector: NM_024635

Applied Biosystems Silencer® siRNAs for MAK10

Sigma-Aldrich siRNA for MAK10

Sigma-Aldrich shRNA for MAK10

Explore Sigma-Aldrich super-pooled esiRNAs

Clones:		Browse Clone Ranger at Invitrogen for clones
		Browse Clones for the Expression of Recombinant Proteins Available from Millipore

OriGene GFP tagged cDNA clone in CMV expression vector: NM_024635
Myc/DDK tagged cDNA clone in CMV expression vector: NM_024635
untagged cDNA clone in CMV expression vector: NM_024635

Primers:

Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers

OriGene genome-wide validated SYBR primer pairs: NM_024635

UniProtKB/Swiss-Prot: MAK10_HUMAN, Q5VZE5

Function: Component of NAT13-MAK10 complex, which probably displays alpha (N-terminal)

acetyltransferase activity. Regulates proliferation of smooth muscle cells (By similarity)

1 Gene Ontology (GO) molecular function term (links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005515	protein binding	ISS	--

About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to ¹UniProtKB, ²MINT, and/or ³STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

2 Interacting proteins for MAK10 (ENSP00000354972³) via UniProtKB, MINT, and/or STRING

Interactant		Interaction Details
GeneCard	External ID(s)	Interaction Details
NAT12	ENSP00000298406³	STRING (score=.73)
NARG1	ENSP00000296543³	STRING (score=.708)

About this table

1 Gene Ontology (GO) biological process term (links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0048659	smooth muscle cell proliferation	ISS	--

About this table

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences

Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for MAK10

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib. RNA:		Invitrogen RNAi Products for gene knock-down (MAK10)
		Browse for Gene Knock-down Tools from Millipore
		Browse Abnova for Chimera RNAi Products

OriGene 29mer shRNA kit in GFP-retroviral vector: NM_024635

Sigma-Aldrich siRNA for MAK10

Sigma-Aldrich shRNA for MAK10

Explore Sigma-Aldrich super-pooled esiRNAs

Applied Biosystems Silencer® siRNAs:

NM_024635

REFSEQ mRNAs for MAK10 gene:

NM_024635.3

Applied Biosystems TaqMan ® Gene Expression Assays:

NM_024635

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_024635
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_024635
                                 untagged cDNA clone in CMV expression vector: NM_024635

Additional cDNA sequence:

AK025266.1 AK026296.1 AK056059.1 AK225237.1 AK292239.1 BC117427.1 BC117429.1

7 DOTS entries:

DT.218694 DT.413781 DT.121208178 DT.95180277 DT.92420200 DT.40220171 DT.92039121

24/71 AceView cDNA sequences (see all 71 ):

BM979022 AK026296 CB409642 AL707881 AI800650 AI697370 AI469401 AA770677

BX954704 AA888870 BM979511 AW169962 AA480211 AW090031 BM699453 NM_024635

AW007583 BG112812 N31792 AK056059 BE504263 BQ007055 AA767659 BU737953

highest scoring ESTs for MAK10:

AI469401 AA188955 AA290566 AA397468 AA480211 AA700321 AA730506 AA769434 AA770677 AA772636

Unigene Cluster for MAK10:

MAK10 homolog, amino-acid N-acetyltransferase subunit (S. cerevisiae)

Hs.436098 [show with all ESTs]

Unigene Representative Sequence: NM_024635

GeneLoc Exon Structure

3 Ensembl transcripts including schematic representations:

ENST00000376041 ENST00000376040 ENST00000361671

(Experimental results according to ¹GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to ²GeneAnnot , ³GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

MAK10 expression in normal and diseased human tissues

Applied Biosystems TaqMan ® Gene Expression Assays for MAK10

¹ / ² / ³

7 probe-sets matching MAK10 gene

Affymetrix probe-set	Array	# genes	Sensitivity	Specificity	Correlation	Length	Gb_Accession	Consensus	Uniqueness	Score	Rank
Affymetrix probe-set	Array	GeneAnnot data			GeneNote data		GeneTide data
46994_at²^{, 3}	U95-B	1	1.00	1.00	0.83	1.00	AI469401	1.00	1.00	1.00	1
91259_i_at²^{, 3}	U95-E	1	1.00	1.00	0.83	0.99	AA947117	0.80	1.00	0.91	1
45226_s_at²^{, 3}	U95-B	1	1.00	1.00	0.66	1.02	AA769434	0.80	1.00	0.91	1
220925_at²^{, 3}	U133-A	1	1.00	1.00	--	--	NM_021929	0.20	1.00	0.72	1
219362_at²^{, 3}	U133-A	1	1.00	1.00	--	--	NM_024635	0.60	1.00	0.82	1
220925_at²	U133Plus2	1	1.00	1.00	--	--	--	--	--	--	--
219362_at²	U133Plus2	1	1.00	1.00	--	--	--	--	--	--	--

GeneDecks MAK10 for binary patterns associated with the probe-sets selected above

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Data from

(Publications) and GNF BioGPS
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About these images

CGAP SAGE TAG: GAAGAAACTG

SOURCE GeneReport for Unigene cluster: Hs.436098

(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD and/or ⁵MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section

Orthologs for MAK10 gene from 5/15 species (see all 15 )

Organism	Gene	Locus	Description	Human Similarity	NCBI accessions
dog (Canis familiaris)	MAK10¹	--	MAK10 homolog, amino-acid N-acetyltransferase subunit more	94.57(n) 99.31(a)	476304 XM_533507.2 XP_533507.2
chimpanzee (Pan troglodytes)	MAK10¹	--	MAK10 homolog, amino-acid N-acetyltransferase subunit more	99.82(n) 100(a)	472971 XM_001136900.1 XP_001136900.1
cow (Bos taurus)	MAK10¹	--	MAK10 homolog, amino-acid N-acetyltransferase subunit more	95.26(n) 99.17(a)	506175 XM_868391.2 XP_873484.2
rat (Rattus norvegicus)	Mak10¹	--	MAK10 homolog, amino-acid N-acetyltransferase subunit, more	88.23(n) 96.14(a)	64472 NM_133324.1 NP_579858.1
mouse (Mus musculus)	Mak10^{1, 5}	13⁵	MAK10 homolog, amino-acid N-acetyltransferase subunit, more^{1, 5}	88.87(n)¹ 97.1(a)¹	78689¹ NM_030153.1¹ NP_084429.1¹ AI158944⁵ AK021042⁵ (see all 26)

About this table Species with no ortholog for MAK10

ENSEMBL Gene Tree for MAK10

(Paralogs according to ¹HomoloGene
and ²Ensembl, Pseudogenes according to ³Pseudogene.org)
About This Section

(According to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section

10/305 NCBI SNPs in MAK10 are shown (see all 305 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay) (see all 90)

AB	SNP ID	Valid	Chr 9 pos	Sequence	Recs	AA Chg	Type	More	Recs	Allele freq	Pop	Total sample	More
AB	Genomic Data				Transcription Data				Allele Frequencies
				--	--	--		--	--				--
	rs7450^1,2	A,C,F,H,O	87826707(+)	`CTGGGG/AAGGTG`	1	--	ut3¹ ese³		6		MN EU EA WA	790
--	rs10868353^1,2	A	87743903(+)	`TTTTTA/GGTAGA`	1	--	ng5¹		0	--	--	--	--
	rs4418412^1,2	H	87826749(+)	`CAACTC/TTTAGA`	1	--	ut3¹		4		EU EA WA	396
	rs13289420^1,2	H	87827062(+)	`GTAGGT/ACTTTG`	1	--	ng3¹		4		EU EA WA	406
	rs10868361^1,2	A,C,F,H	87812427(+)	`TCCAGC/TGTAAG`	1	--	int¹		10		EU EA WA	1016
	rs10868356^1,2	A,C,F,H	87766551(+)	`TGATTG/TATGTA`	1	--	int¹		8		EU EA WA NA	624
	rs10868362^1,2	A,C,F,H	87824482(+)	`agaggC/Tgtagc`	1	--	int¹		4		EU EA WA	418
	rs10868357^1,2	A,C,F,H	87787082(+)	`AAAAAA/CTCCAG`	1	--	int¹		7		NA EU EA WA	560
	rs4877955^1,2	A,C,F,H	87795025(+)	`CATCTG/TCCCCC`	1	--	int¹		7		EU EA WA NA	560
	rs4877956^1,2	A,C,F,H	87797808(+)	`ACATGC/GAACAT`	1	--	int¹		11		EU EA WA NA	980

About this table

HapMap Linkage Disequilibrium images for MAK10 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

(Possibly Related Articles in Doctor's Guide)
About This Section

(in PubMed. Associations of this gene to articles via ¹Novoseek, ²HGNC, ³Entrez Gene, ⁴UniProtKB/Swiss-Prot, ⁵UniProtKB/TrEMBL, ⁶GAD, and/or ⁷PharmGKB)
About This Section

10 PubMed articles for MAK10 gene:

Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)^{2, 3, 4} Ota T....Sugano S. (2004)
Embryonic growth-associated protein is one subunit of a novel N- terminal acetyltransferase complex essential for embryonic vascular development. (PubMed id 16484612)⁴ Wenzlau J.M.... Weiser-Evans M.C.M. (2006)
Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. (PubMed id 16344560)³ Kimura K.... Sugano S. (2006)
The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)⁴ Gerhard D.S....Malek J. (2004)
DNA sequence and analysis of human chromosome 9. (PubMed id 15164053)⁴ Humphray S.J.... Dunham I. (2004)
Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)³ Strausberg R.L....Marra M.A. (2002)
A novel epithelial wound-related gene is abundantly expressed in developing rat cornea and skin. (PubMed id 10855038)³ Yi X.J....Yu F.S. (2000)
Construction and characterization of a full length-enriched and a 5'- end-enriched cDNA library. (PubMed id 9373149)³ Suzuki Y.... Sugano S. (1997)
Normalization and subtraction: two approaches to facilitate gene discovery. (PubMed id 8889548)³ Bonaldo M.F....Soares M.B. (1996)
Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides. (PubMed id 8125298)³ Maruyama K. and Sugano S. (1994)

(in PubMed, OMIM, and NCBI Bookshelf)
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		AND	OR
Aliases
Free Text

Query String		PubMed OMIM NCBI Bookshelf
	(Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
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Entrez Gene: 60560	HGNC: 24340	AceView: MAK10	Ensembl:ENSG00000135040	euGenes: HUgn60560
ECgene: MAK10	H-InvDB: MAK10

(According to HUGE)
About This Section

(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

Licensable Technologies for MAK10
(Available from WIS Yeda, Salk, Tufts)

About This Section

(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section

Products for MAK10:

TaqMan ® Gene Expression Assays

TaqMan ® Genotyping Assays

Free SNP selection tool

Invitrogen iPath Pathways	Invitrogen BLOCK-iT™ RNAi
Invitrogen Antibodies	Invitrogen Assays
Invitrogen Clones	Invitrogen Q-PCR Products
Invitrogen Human Recombinant Kinases	Invitrogen Custom Antibody and Peptide Service
Invitrogen Proteins / Assays / Screening Services	Search Invitrogen catalog for MAK10-related products

	Millipore Custom Antibody & Bulk Services
	Millipore Preclinical / Clinical Development Services
	Millipore Immunoassay Services
	Millipore Target Screening & Profiling Services

	Predesigned and custom siRNAs for MAK10		Antibodies for MAK10
	Explore super-pooled esiRNAs		Browse proteins at Sigma-Aldrich
	Lentivirus-delivered shRNAs for MAK10		Browse small molecules at Sigma-Aldrich
	"Your Favorite Gene" Pathways		feedback

	Antibodies		Primer Pairs
	Cell Culture Products		ELISAs
	Flow Cytometry Kits		Protease Activity Assays & Reagents
	Cell Selection/Detection Kits & Reagents		ELISA/Assay Development Kits & Reagents
	Multiplex/Array Assay Kits & Reagents		ELISpot Kits & Development Modules
	Recombinant & Natural Proteins

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	Custom Service for Rabbit Pab from Full-length Protein

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Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies

Search Novus for antibodies for MAK10

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MAK10 Gene

protein-coding GIFtS: 43

Aliases &
Descriptions
for MAK10

Summaries
for MAK10

Genomic Location
for MAK10

Proteins
for MAK10

Protein Domains/
Families
for MAK10

Gene Function
for MAK10

Pathways & Interactions
for MAK10

Drugs & Compounds
for MAK10

Transcripts
for MAK10

Expression
for MAK10

Orthologs
for MAK10

Paralogs
for MAK10

SNPs/Variants
for MAK10

Disorders & Mutations
for MAK10

Medical News
for MAK10

Publications
for MAK10

Search for MAK10

Genome Databases showing MAK10

Other Databases showing MAK10

Specialized Databases showing MAK10

Licensable Technologies
for MAK10

Services
for MAK10

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

MAK10 Gene

protein-coding GIFtS: 43

Aliases & Descriptionsfor MAK10

Summariesfor MAK10

Genomic Locationfor MAK10

Proteinsfor MAK10

Protein Domains/Familiesfor MAK10

Gene Functionfor MAK10

Pathways & Interactionsfor MAK10

Drugs & Compoundsfor MAK10

Transcriptsfor MAK10

Expression for MAK10

Orthologsfor MAK10

Paralogsfor MAK10

SNPs/Variantsfor MAK10

Disorders & Mutationsfor MAK10

Medical Newsfor MAK10

Publicationsfor MAK10

Search for MAK10

Genome Databases showing MAK10

Other Databases showing MAK10

Specialized Databases showing MAK10

Licensable Technologiesfor MAK10

Servicesfor MAK10

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

Aliases &
Descriptions
for MAK10

Summaries
for MAK10

Genomic Location
for MAK10

Proteins
for MAK10

Protein Domains/
Families
for MAK10

Gene Function
for MAK10

Pathways & Interactions
for MAK10

Drugs & Compounds
for MAK10

Transcripts
for MAK10

Expression
for MAK10

Orthologs
for MAK10

Paralogs
for MAK10

SNPs/Variants
for MAK10

Disorders & Mutations
for MAK10

Medical News
for MAK10

Publications
for MAK10

Licensable Technologies
for MAK10

Services
for MAK10