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MAGEE2 Gene

protein-coding   GIFtS: 45
GCID: GC0XM075002

Melanoma Antigen Family E, 2

  See related diseases
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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Aliases
Melanoma Antigen Family E, 21 2
Hepatocellular Carcinoma-Associated Protein 32 3
HCA32 3
MAGE-E2 Antigen2 3
Hepatocellular Carcinoma-Associated Protein HCA32
Melanoma-Associated Antigen E22

External Ids:    HGNC: 249351   Entrez Gene: 1395992   Ensembl: ENSG000001866757   OMIM: 3007605   UniProtKB: Q8TD903   

Export aliases for MAGEE2 gene to outside databases

Previous GC identifers: GC0XM073870 GC0XM074785 GC0XM074919 GC0XM068636


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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Entrez Gene summary for MAGEE2 Gene:
This gene encodes a member of the E subfamily of MAGE (melanoma antigen-encoding gene) gene family. The gene is
intronless and the encoded protein has two of the MAGE domains which are characteristic of MAGE family proteins.
(provided by RefSeq, Sep 2011)

GeneCards Summary for MAGEE2 Gene:
MAGEE2 (melanoma antigen family E, 2) is a protein-coding gene. Diseases associated with MAGEE2 include hepatocellular carcinoma, and melanoma. An important paralog of this gene is MAGEH1.




(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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RefSeq DNA sequence:
NC_000023.11  NC_018934.2  NT_011651.18  
Regulatory elements:
   Regulatory transcription factor binding sites in the MAGEE2 gene promoter:
         GR   USF1   Sp1   GR-beta   Arnt   POU2F1   USF-1   POU2F1a   Msx-1   GR-alpha   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidMAGEE2 promoter sequence
   Search Chromatin IP Primers for MAGEE2

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat MAGEE2


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: Xq13.3   Ensembl cytogenetic band:  Xq13.3   HGNC cytogenetic band: Xq13

MAGEE2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
MAGEE2 gene location

GeneLoc information about chromosome X         GeneLoc Exon Structure

GeneLoc location for GC0XM075002:  view genomic region     (about GC identifiers)

Start:
75,002,823 bp from pter      End:
75,005,079 bp from pter
Size:
2,257 bases      Orientation:
minus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Cloud-Clone Corp.,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Cloud-Clone Corp., Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, Cloud-Clone Corp, and/or others.)
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UniProtKB/Swiss-Prot: MAGE2_HUMAN, Q8TD90 (See protein sequence)
Recommended Name: Melanoma-associated antigen E2  
Size: 523 amino acids; 60378 Da
Secondary accessions: Q5JSI5

Explore the universe of human proteins at neXtProt for MAGEE2: NX_Q8TD90

Explore proteomics data for MAGEE2 at MOPED


See MAGEE2 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

REFSEQ proteins: NP_619648.1  
ENSEMBL proteins: 
 ENSP00000362457  

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Cloud-Clone Corp. CLIAs for MAGEE2


(According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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1 InterPro protein domain:
 IPR002190 MAGE

Graphical View of Domain Structure for InterPro Entry Q8TD90

ProtoNet protein and cluster: Q8TD90

UniProtKB/Swiss-Prot: MAGE2_HUMAN, Q8TD90
Similarity: Contains 2 MAGE domains


MAGEE2 for domains           About GeneDecksing


(According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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Animal Models:
   inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for MAGEE2
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1 qRT-PCR Assays for microRNA that regulate MAGEE2:
hsa-miR-664
SwitchGear 3'UTR luciferase reporter plasmidMAGEE2 3' UTR sequence
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(According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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Subcellular locations from COMPARTMENTS: 

CompartmentConfidence
cytosol2
nucleus2

(SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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    Custom Pathway & Disease-focused RT2 Profiler PCR Arrays for MAGEE2
Interactions:

    Search GeneGlobe Interaction Network for MAGEE2

STRING Interaction Network Preview (showing 1 interactants - click image to see more details)

Selected Interacting proteins for MAGEE2 (Q8TD902 ENSP000003624574) via UniProtKB, MINT, STRING, and/or I2D (see all 553)
InteractantInteraction Details
GeneCardExternal ID(s)
CDC5LQ994592, ENSP000003605324MINT-7947479 STRING: ENSP00000360532
ABCF1Q8NE712MINT-7947479
ABCF3Q9NUQ82MINT-7947479
ACTBP607092MINT-7947479
ACTL6AO960192MINT-7947479
About this table

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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Browse Small Molecules at EMD Millipore
   Browse drugs & compounds from Enzo Life Sciences
  Browse compounds at ApexBio 

Browse Tocris compounds for MAGEE2 (MAGE2)



(Secondary structures according to fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, and/or QIAGEN )
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REFSEQ mRNAs for MAGEE2 gene: 
NM_138703.4  

Unigene Cluster for MAGEE2:

Melanoma antigen family E, 2
Hs.356869  [show with all ESTs]
Unigene Representative Sequence: NM_138703
1 Ensembl transcript including schematic representation, and UCSC links where relevant:
ENST00000373359(uc004ecj.2)
miRNA
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1 qRT-PCR Assays for microRNA that regulate MAGEE2:
hsa-miR-664
SwitchGear 3'UTR luciferase reporter plasmidMAGEE2 3' UTR sequence
Inhib. RNA
Products:
     
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Predesigned siRNA for gene silencing in human, mouse, rat MAGEE2
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Primer
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  QuantiFast Probe-based Assays in human, mouse, rat MAGEE2

Additional mRNA sequence: 

AF490509.1 AK094624.1 BC093964.1 BC111949.1 

1 DOTS entry:

DT.40109913 

18 AceView cDNA sequences:

AK094624 F01615 NM_138703 CR624393 AF490509 AA447180 CD636475 CD636477 
F05356 AL539705 CD636483 BX433215 CD636479 CD636491 CD636489 CD636482 
CD636487 AL568006 

GeneLoc Exon Structure


(RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
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MAGEE2 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS <intensity>2/3
CGAP TAG: TTTTTCCTTG
MAGEE2 Expression
About this image

MAGEE2 Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

MAGEE2 Protein Expression

SOURCE GeneReport for Unigene cluster: Hs.356869
    Custom PCR Arrays for MAGEE2
Primer
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In Situ
Assay Products:
 

 
Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for MAGEE2

(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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This gene was present in the common ancestor of human and mouse.

Orthologs for MAGEE2 gene from Selected species (see all 5)    About this table
Organism Taxonomic
classification
Gene Description Human
Similarity
Orthology
Type
Details
mouse
(Mus musculus)
Mammalia Magee21 , 5 melanoma antigen, family E, 21, 5 86.22(n)1
84.04(a)1
  X (46.77 cM)5
2727901  NM_053206.21  NP_444436.11 
 1048549525 


ENSEMBL Gene Tree for MAGEE2 (if available)
TreeFam Gene Tree for MAGEE2 (if available)

(Paralogs according to 1HomoloGene,
2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
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Paralogs for MAGEE2 gene
MAGEH12  MAGEE12  MAGEC32  
8 SIMAP similar genes for MAGEE2 using alignment to 1 protein entry:     MAGE2_HUMAN:
MAGEH1    NDNL2    MAGEF1    MAGEB2    MAGEB3    MAGEA8
NDN    MAGEC4

MAGEE2 for paralogs           About GeneDecksing



(SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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Selected SNPs for MAGEE2 (see all 130)    About this table    
Genomic DataTranscription Related DataAllele Frequencies
SNP IDValidClinical
significance
Chr X posSequence#AA
Chg
TypeMore#Allele
freq
PopTotal
sample
More
----------
rs1438504271,2
C,Funtested175011581(+) GTTGCA/C/TCATCA 2 S G mis11NA 4550
rs1815693401,2
--75010292(+) AAATTA/CTTCAC 1 -- ds50010--------
rs1395013881,2
--75010367(+) TCTTAA/CAATAG 1 -- ds50010--------
rs681367141,2
C,F--75010553(+) TTGTGA/GACTCT 1 -- ds50012Minor allele frequency- G:0.25WA 4
rs1852738811,2
--75010600(+) CCATAA/GCATAA 1 -- ds50010--------
rs1915013091,2
--75010634(+) GAGCCC/TTCCCC 1 -- ds50010--------
rs1473491421,2
--75010716(+) TGGCAA/GGTGCT 1 -- ds50010--------
rs169920931,2
C,F,H--75011027(+) GACCGG/TGATGG 1 -- ut3111Minor allele frequency- T:0.02NA NS EA 1214
rs1825234071,2
C--75011280(+) TGATAA/GTTGCC 2 T I mis10--------
rs1501537531,2
F--75011303(+) CTGGCC/TTCCTC 2 E syn11Minor allele frequency- T:0.00NA 4550

HapMap Linkage Disequilibrium report for MAGEE2 (75002823 - 75005079 bp)

Structural Variations
     Database of Genomic Variants (DGV) 1 variation for MAGEE2:    About this table    
Variant IDTypeSubtypePubMed ID
esv2752334CNV Gain17911159

Human Gene Mutation Database (HGMD): MAGEE2
Locus Specific Mutation Databases (LSDB): MAGEE2

Site Specific Mutation Identification with PCR Assays
SeqTarget long-range PCR primers for resequencing MAGEE2
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(in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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OMIM gene information: 300760    OMIM disorders: --

2 diseases for MAGEE2:    
About MalaCards
hepatocellular carcinoma    melanoma


MAGEE2 for disorders           About GeneDecksing


Export disorders for MAGEE2 gene to outside databases

(in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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PubMed articles for MAGEE2 gene integrated from 10 sources:
(articles sorted by number of sources associating them with MAGEE2)
    Utopia: connect your pdf to the dynamic
world of online information

  1. The DNA sequence of the human X chromosome. (PubMed id 15772651)1, 2 Ross M.T.... Bentley D.R. (Nature 2005)
  2. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S.... Malek J. (Genome Res. 2004)
  3. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (Nat. Genet. 2004)
  4. An overview of the MAGE gene family with the identification of all human members of the family. (PubMed id 11454705)1, 3 Chomez P.... Lucas S. (Cancer Res. 2001)
  5. Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. (PubMed id 16344560)1 Kimura K.... Sugano S. (Genome Res. 2006)
  6. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1 Strausberg R.L....Marra M.A. (Proc. Natl. Acad. Sci. U.S.A. 2002)
  7. (PubMed id )2 

(in PubMed, OMIM, and NCBI Bookshelf)
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 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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Entrez Gene: 139599 HGNC: 24935 AceView: MAGEE2 Ensembl:ENSG00000186675 euGenes: HUgn139599
ECgene: MAGEE2 H-InvDB: MAGEE2

(According to HUGE)
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  --

(According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
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NameDescription
PharmGKB entry for MAGEE2 Pharmacogenomics, SNPs, Pathways
ATLAS Chromosomes in Cancer entry for MAGEE2 Genetics and Cytogenetics in Oncology and Haematology

(Patent information from GeneIP,
Licensable technologies from WIS Yeda, Salk, Tufts,
IP news from LifeMap Sciences, Inc.)
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Patent Information for MAGEE2 gene:
Search GeneIP for patents involving MAGEE2

GeneCards and IP:
Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript, and ESI BIO, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
Advanced Cell Diagnostics, Animal models from inGenious Targeting Laboratory, genOway)
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GeneCards Homepage - Last full update: 7 May 2014 - Incrementals: 9 May 2014 , 2 Jun 2014 , 26 Jun 2014 , 30 Jun 2014

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The GeneCards human gene database gene index: 1 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 


Developed at the Crown Human Genome Center, Department of Molecular Genetics, the Weizmann Institute of Science

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