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MAGEE2 Gene

protein-coding   GIFtS: 41

GC0XM074919
melanoma antigen family E, 2
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
HCA3 1, 2, 3
MGC138154 2
OTTHUMP00000023584 2
Descriptions
Hepatocellular carcinoma-associated protein 3 3
MAGE-E2 antigen 3
hepatocellular carcinoma-associated protein HCA3 2
melanoma antigen family E, 2 2
External Ids
HGNC: 249351
Entrez Gene: 1395992
UniProtKB: Q8TD903
Ensembl: ENSG000001866757
Search outside databases for aliases for MAGEE2 gene

Previous GC identifers: GC0XM073870 GC0XM074785

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

  --
(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
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Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the MAGEE2 gene  

Entrez Gene cytogenetic band: Xq13.3   Ensembl cytogenetic band:  Xq13.3   HGNC cytogenetic band: Xq13

MAGEE2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome X         GeneLoc Exon Structure

GeneLoc location for GC0XM074919:     (about GC identifiers)

Start:
74,919,548 bp from pter
End:
74,921,796 bp from pter
Size:
2,249 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000023.9  NT_011669.16  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: MAGE2_HUMAN, Q8TD90 (See protein sequence)
Recommended Name: Melanoma-associated antigen E2  
Size: 523 amino acids; 60378 Da
Secondary accessions: Q5JSI5

REFSEQ proteins: NP_619648.1  

ENSEMBL proteins: 
ENSP00000362457 


Human Recombinant Proteins 
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Browse recombinant and purified proteins available from Enzo Life Sciences
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Browse Abnova for Human Recombinant Proteins
                Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 


Antibodies for MAGEE2: 
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Search for antibodies at Abcam and the World's Antibody Gateway (free search engine of over 150 antibody companies)
Browse Abnova for Monoclonal and Polyclonal Antibodies
Search Novus for antibodies for MAGEE2

Assays for MAGEE2: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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1 InterPro domain/family:
 IPR002190 MAGE


   GeneDecks  MAGEE2 for the domains selected above  
About GeneDecksing

Graphical View of Domain Structure for InterPro Entry Q8TD90

ProtoNet protein and cluster: Q8TD90

UniProtKB/Swiss-Prot: MAGE2_HUMAN, Q8TD90
Similarity: Contains 2 MAGE domains

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (MAGEE2)
Browse for Gene Knock-down Tools from Millipore
Browse Abnova for Chimera RNAi Products
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_138703

              Applied Biosystems Silencer® siRNAs for MAGEE2

              Sigma-Aldrich siRNA for MAGEE2  
                     Sigma-Aldrich shRNA for MAGEE2  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Browse Clone Ranger at Invitrogen for clones
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_138703
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_138703
                                 untagged cDNA clone in CMV expression vector: NM_138703 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_138703

1 MGI phenotypic allele for Magee2 (no phenotypes)

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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  --
(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for MAGEE2

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (MAGEE2)
Browse for Gene Knock-down Tools from Millipore
Browse Abnova for Chimera RNAi Products
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_138703

              Sigma-Aldrich siRNA for MAGEE2  
                     Sigma-Aldrich shRNA for MAGEE2  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_138703  

REFSEQ mRNAs for MAGEE2 gene: 

NM_138703.3   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_138703  

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_138703
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_138703
                                 untagged cDNA clone in CMV expression vector: NM_138703 

Additional cDNA sequence: 

AF490509.1 AK094624.1 BC093964.1 BC111949.1 CR624393.1 

1 DOTS entry:

DT.40109913 

18 AceView cDNA sequences:

AA447180 F01615 NM_138703 AF490509 AK094624 CR624393 AL539705 CD636483 
CD636475 BX433215 CD636477 F05356 CD636479 CD636491 CD636489 CD636482 
CD636487 AL568006 

highest scoring ESTs for MAGEE2:

AA447180 AF490509 AK094624 F05356 AL539705 BC093964 BX433215 CD636475 CD636477 CD636479 

Unigene Cluster for MAGEE2:

Melanoma antigen family E, 2
Hs.356869  [show with all ESTs]
Unigene Representative Sequence: NM_138703


GeneLoc Exon Structure

1 Ensembl transcript including schematic representation:
ENST00000373359  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
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MAGEE2 expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for MAGEE2

1 / 2

1 probe-set matching MAGEE2 gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

1553254_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
About this table
Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: TTTTTCCTTG

SOURCE GeneReport for Unigene cluster: Hs.356869
(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for MAGEE2 gene from 5 species
Organism Gene Locus Description Human
Similarity
NCBI accessions
dog
(Canis familiaris)
MAGEE21   -- melanoma antigen family E, 2 89.14(n)
86.78(a)
480961  XM_538082.2  XP_538082.2 
chimpanzee
(Pan troglodytes)
MAGEE21   -- melanoma antigen family E, 2 99.35(n)
98.79(a)
465724  XM_521144.2  XP_521144.2 
cow
(Bos taurus)
MAGEE21   -- melanoma antigen family E, 2 89.46(n)
86.97(a)
525318  NM_001076876.1  NP_001070344.1 
rat
(Rattus norvegicus)
Magee21   -- melanoma antigen, family E, 2 86.26(n)
83.62(a)
302392  XM_228532.3  XP_228532.1 
mouse
(Mus musculus)
Magee21, 5 X5
melanoma antigen, family E, 21, 5 86.32(n)1
84.2(a)1
2727901  NM_053206.11  NP_444436.11 
 AA4441995  AF3199825  (see all 13)
About this table        Species with no ortholog for MAGEE2

ENSEMBL Gene Tree for MAGEE2
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

Paralogs for MAGEE2 gene
MAGEH12  NDNL22  MAGEE12  NDN2  MAGEF12  

(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
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10/17 NCBI SNPs in MAGEE2 are shown (see all 17 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 16)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr X posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
rs13438791,2
A,C,F,H74921254(+) GTACTC/ATCTGA 1 X/E non1 ese313Minor allele frequency- A:0.41NA EA EU WA 1157
rs44302421,2
C,F,H74922637(+) AGGGTC/GAATTA 1 -- ng31 tfbs312Minor allele frequency- G:0.07NS EU EA WA NA 664
rs169920961,2
F,H74923200(+) TTGCAT/GTTTTC 1 -- ng317Minor allele frequency- G:0.04NA EU EA WA 536
rs126886001,2
C,H74920741(+) GTCACC/ATTCCA 1 C/G mis14Minor allele frequency- A:0.00EU EA WA 406
rs169920931,2
F,H74919815(+) GACCGG/TGATGG 1 -- ut317Minor allele frequency- T:0.01NA EU EA WA 514
rs48926161,2
C74923644(+) CTTTAA/CCCTTC 1 -- ng31 tfbs30--------
rs617324401,2
--74920819(+) GGCTTA/GCATCT 2 E/Q E/X mis1 non10--------
--
rs617324411,2
--74921479(+) GCACTA/CGGGGG 2 Q/E Q/X mis1 non10--------
--
rs681367141,2
--74919341(+) TTGTGA/GACTCT 1 -- ng510--------
--
rs413106791,2
--74920423(+) TCTCCC/TCAGGC 1 G/R mis10--------
About this table

HapMap Linkage Disequilibrium images for MAGEE2 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 300760

Human Gene Mutation Database: MAGEE2

(Possibly Related Articles in Doctor's Guide)
About This Section

  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
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6 PubMed articles for MAGEE2 gene:
(in PubMed, OMIM, and NCBI Bookshelf)
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 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
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Entrez Gene: 139599 HGNC: 24935 AceView: MAGEE2 Ensembl:ENSG00000186675 euGenes: HUgn139599
ECgene: MAGEE2 H-InvDB: MAGEE2
(According to HUGE)
About This Section

  --
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

NameDescription
ATLAS Chromosomes in Cancer entry for MAGEE2 Genetics and Cytogenetics in Oncology and Haematology
(Available from WIS Yeda, Salk, Tufts)
About This Section

  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for MAGEE2:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



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 Predesigned and custom siRNAs for MAGEE2 Browse antibodies at Sigma-Aldrich
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 Lentivirus-delivered shRNAs for MAGEE2 Browse small molecules at Sigma-Aldrich
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