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MAGEE1 Gene

protein-coding GIFtS: 53

GC0XP075564

melanoma antigen family E, 1
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc , and/or ⁷Ensembl, ⁸miRBase)
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Aliases
DAMAGE ^¹, ², ⁵
HCA1 ^², ³
KIAA1587 ^¹, ², ³, ⁵
OTTHUMP00000023588 ^²

Descriptions
Hepatocellular carcinoma-associated protein 1 ^³
MAGE-E1 antigen ^³
dystrobrevin-associated MAGE protein ^²
hepatocellular carcinoma-associated HCA1 ^²
melanoma antigen family E, 1 ^²

External Ids
HGNC: 24934¹
Entrez Gene: 57692²
UniProtKB: Q9HCI5³
Ensembl: ENSG00000198934⁷

Search outside databases for aliases for MAGEE1 gene

Previous GC identifers: GC0XM135820 GC0XM138147 GC0XM139216 GC0XM139976 GC0XP074516 GC0XP075430

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:

UCSC Golden Path with GeneCards custom track

Transcription factor binding sites upstream to the MAGEE1 gene

Entrez Gene cytogenetic band: Xq13.3 Ensembl cytogenetic band: Xq13.3 HGNC cytogenetic band: Xq13

MAGEE1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome X GeneLoc Exon Structure

GeneLoc location for GC0XP075564: (about GC identifiers)

Start:	75,564,521 bp from pter
End:	75,568,148 bp from pter
Size:	3,628 bases
Orientation:	plus strand

RefSeq DNA sequence:

NC_000023.9 NT_011669.16

(According to ¹UniProtKB, and/or Ensembl, Phosphorylation sites according to ²Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: MAGE1_HUMAN, Q9HCI5 (See protein sequence)

Recommended Name: Melanoma-associated antigen E1

Size: 957 amino acids; 103254 Da

PDB structures from and Proteopedia :

2H5T (3D)

Secondary accessions: Q86TG0 Q8TD92 Q9H216

Post-translational modifications:

View phosphorylation sites using PhosphoSite²

REFSEQ proteins: NP_065983.1

ENSEMBL proteins:

ENSP00000354912

Human Recombinant Proteins

	Browse Drug Discovery Central at Invitrogen for human recombinant proteins
	Browse Purified and Recombinant Proteins at Millipore
	Browse Human Recombinant Proteins at Sigma-Aldrich
	Browse R&D Systems for human recombinant proteins
	Browse recombinant and purified proteins available from Enzo Life Sciences
	Browse Abcam for Recombinant Proteins
	Human Recombinant Proteins from Abnova (MAGEE1)

Browse Origene for full length recombinant human proteins expressed in human HEK293 cells

5/8 Gene Ontology (GO) cellular component terms (links to tree view) (see all 8 ):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005634	nucleus	ISS	14623885
GO:0005737	cytoplasm	IDA	18029348
GO:0005856	cytoskeleton	IDA	18029348
GO:0005886	plasma membrane	ISS	14623885
GO:0016010	colocalizes with dystrophin-associated glycoprotein complex	ISS	14623885

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Antibodies for MAGEE1:

	Browse Antibodies Central at Invitrogen
	Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
	Sigma-Aldrich Antibodies for MAGEE1
	Browse R&D Systems for Antibodies
	Search for antibodies at Abcam and the World's Antibody Gateway (free search engine of over 150 antibody companies)
	Monoclonal and Polyclonal Antibodies from Abnova (MAGEE1)
	Novus Biologicals Antibody for MAGEE1

Assays for MAGEE1:

	Browse Invitrogen for biochemical assays
	Browse Kits and Assays available from Millipore
	Browse R&D Systems for biochemical assays
	Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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Graphical View of Domain Structure for InterPro Entry Q9HCI5

ProtoNet protein and cluster: Q9HCI5

1 Blocks protein family: IPB002190 MAGE protein

UniProtKB/Swiss-Prot: MAGE1_HUMAN, Q9HCI5

Similarity: Contains 2 MAGE domains

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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Inhib. RNA:		Invitrogen RNAi Products for gene knock-down (MAGEE1)
		Browse for Gene Knock-down Tools from Millipore
		Browse Abnova for Chimera RNAi Products

OriGene 29mer shRNA kit in GFP-retroviral vector: NM_020932

Applied Biosystems Silencer® siRNAs for MAGEE1

Sigma-Aldrich siRNA for MAGEE1

Sigma-Aldrich shRNA Panels and shRNA for MAGEE1

Explore Sigma-Aldrich super-pooled esiRNAs

Clones:		Invitrogen Clones for MAGEE1
		Browse Clones for the Expression of Recombinant Proteins Available from Millipore

OriGene GFP tagged cDNA clone in CMV expression vector: NM_020932
Myc/DDK tagged cDNA clone in CMV expression vector: NM_020932
untagged cDNA clone in CMV expression vector: NM_020932

Primers:

Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers

OriGene genome-wide validated SYBR primer pairs: NM_020932

1 Gene Ontology (GO) molecular function term (links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005515	protein binding	ISS	14623885

About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to ¹UniProtKB, ²MINT, and/or ³STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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5/6 Interacting proteins for MAGEE1 (ENSP00000354912³) via UniProtKB, MINT, and/or STRING (see all 6 )

Interactant		Interaction Details
GeneCard	External ID(s)	Interaction Details
GADD45G	ENSP00000252506³	STRING (score=.839)
GADD45A	ENSP00000360025³	STRING (score=.802)
GADD45B	ENSP00000215631³	STRING (score=.8)
TP53	ENSP00000269305³	STRING (score=.736)
DDIT4L	ENSP00000354830³	STRING (score=.728)

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1 Gene Ontology (GO) biological process term (links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0008150	biological_process	ND	--

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(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences

Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for MAGEE1

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib. RNA:		Invitrogen RNAi Products for gene knock-down (MAGEE1)
		Browse for Gene Knock-down Tools from Millipore
		Browse Abnova for Chimera RNAi Products

OriGene 29mer shRNA kit in GFP-retroviral vector: NM_020932

Sigma-Aldrich siRNA for MAGEE1

Sigma-Aldrich shRNA Panels and shRNA for MAGEE1

Explore Sigma-Aldrich super-pooled esiRNAs

Applied Biosystems Silencer® siRNAs:

NM_020932

REFSEQ mRNAs for MAGEE1 gene:

NM_020932.1

Applied Biosystems TaqMan ® Gene Expression Assays:

NM_020932

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_020932
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_020932
                                 untagged cDNA clone in CMV expression vector: NM_020932

Additional cDNA sequence:

AB046807.1 AF086039.1 AF320909.1 AF490507.1 AK291270.1 BC050588.1

2 DOTS entries:

DT.410580 DT.121323942

24/28 AceView cDNA sequences (see all 28 ):

AF490507 AI298393 AB046807 NM_020932 AF320909 AF086039 AI124583 BC050588

N22111 BX107699 T41267 N66646 AA448930 CD630472 BP362794 AA449441

BQ717147 AI912696 CD512856 T40424 AA189100 AI281387 W26514 W28145

highest scoring ESTs for MAGEE1:

AI912696 AA189100 AA448930 AA449441 AB046807 AF086039 AF320909 AF490507 AI124583 AI281387

Unigene Cluster for MAGEE1:

Melanoma antigen family E, 1

Hs.8453 [show with all ESTs]

Unigene Representative Sequence: AB046807

GeneLoc Exon Structure

1 Ensembl transcript including schematic representation:

ENST00000361470

(Experimental results according to ¹GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to ²GeneAnnot , ³GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

MAGEE1 expression in normal and diseased human tissues

Applied Biosystems TaqMan ® Gene Expression Assays for MAGEE1

¹ / ² / ³

4 probe-sets matching MAGEE1 gene

Affymetrix probe-set	Array	# genes	Sensitivity	Specificity	Correlation	Length	Gb_Accession	Consensus	Uniqueness	Score	Rank
Affymetrix probe-set	Array	GeneAnnot data			GeneNote data		GeneTide data
52747_at²^{, 3}	U95-B	1	1.00	1.00	1.00	1.00	AI912696	1.00	1.00	1.00	1
229286_at²^{, 3}	U133-B	1	1.00	1.00	--	--	AI912696	1.00	1.00	1.00	1
1556047_s_at²	U133Plus2	1	1.00	1.00	--	--	--	--	--	--	--
229286_at²	U133Plus2	1	1.00	1.00	--	--	--	--	--	--	--

GeneDecks MAGEE1 for binary patterns associated with the probe-sets selected above

About GeneDecksing

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Data from

(Publications) and GNF BioGPS
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About these images

CGAP SAGE TAG: TATTGGGGGG

SOURCE GeneReport for Unigene cluster: Hs.8453

(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD and/or ⁵MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section

Orthologs for MAGEE1 gene from 4 species

Organism	Gene	Locus	Description	Human Similarity	NCBI accessions
chimpanzee (Pan troglodytes)	MAGEC2¹	--	melanoma antigen family C, 2	98.33(n) 97.77(a)	741367 XM_001143922.1 XP_001143922.1
rat (Rattus norvegicus)	Magee1¹	--	melanoma antigen, family E, 1	76.04(n) 68.3(a)	317232 XM_228531.4 XP_228531.2
mouse (Mus musculus)	Magee1^{1, 5}	X⁵	melanoma antigen, family E, 1^{1, 5}	74.38(n)¹ 66.3(a)¹	107528¹ NM_053201.3¹ NP_444431.3¹ AF319978⁵ AI413449⁵ (see all 14)
baker's yeast (Saccharomyces cerevisiae)	DAN4(YJR151C)⁴	10(715730-712245)	Cell wall mannoprotein with similarity to Tir1p, Tir2p, more	--	853616 NP_012685.1

About this table Species with no ortholog for MAGEE1

ENSEMBL Gene Tree for MAGEE1

(Paralogs according to ¹HomoloGene
and ²Ensembl, Pseudogenes according to ³Pseudogene.org)
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Paralogs for MAGEE1 gene

MAGEH1² NDNL2² MAGEE2² NDN² MAGEF1²

(According to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section

10/16 NCBI SNPs in MAGEE1 are shown (see all 16 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay) (see all 21)

AB	SNP ID	Valid	Chr X pos	Sequence	Recs	AA Chg	Type	More	Recs	Allele freq	Pop	Total sample	More
AB	Genomic Data				Transcription Data				Allele Frequencies
				--	--	--		--	--				--
	rs6647995^1,2	A,C,F,H	75563338(+)	`CAATAG/AAGGGT`	1	--	ng5¹		16		EU EA WA NS NA	847
	rs7051260^1,2	A,C	75565744(+)	`GAGGAC/GTTGAG`	1	E/D	mis¹		0	--	--	--	--
--	rs2144455^1,2	A,F	75568511(-)	`tgagcC/Tgagat`	1	--	ng3¹		3		NA EA	252
--	rs7066146^1,2	A,C	75564955(+)	`GGCCCA/CAGCAC`	1	P/P	syn¹ ese³		0	--	--	--	--
--	rs5982146^1,2	--	75565373(+)	`TCGCCA/GCCACT`	1	A/T	mis¹ ese³		0	--	--	--	--
--	rs6647996^1,2	--	75564031(+)	`CAGGCA/TTTCAG`	1	--	ng5¹		0	--	--	--	--
--	rs41298484^1,2	--	75565464(+)	`AAGCAA/CCCCCG`	1	T/N	mis¹		0	--	--	--	--
--	rs67821007^1,2	--	75564348(+)	`CCTACC/TAAAGT`	1	--	ng5¹		0	--	--	--	--
--	rs41307357^1,2	--	75564724(+)	`GGCAGA/GACCAT`	1	--	ut5¹		0	--	--	--	--
--	rs61732405^1,2	--	75565337(+)	`CTACAC/TCTGGT`	1	P/S	mis¹		0	--	--	--	--

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HapMap Linkage Disequilibrium images for MAGEE1 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 300759

(Possibly Related Articles in Doctor's Guide)
About This Section

(in PubMed. Associations of this gene to articles via ¹Novoseek, ²HGNC, ³Entrez Gene, ⁴UniProtKB/Swiss-Prot, ⁵UniProtKB/TrEMBL, ⁶GAD, and/or ⁷PharmGKB)
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8 PubMed articles for MAGEE1 gene:

The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)^{3, 4} Gerhard D.S....Malek J. (2004)
DAMAGE, a novel alpha-dystrobrevin-associated MAGE protein in dystrophin complexes. (PubMed id 14623885)^{2, 3} Albrecht D.E. and Froehner S.C. (2004)
Prediction of the coding sequences of unidentified human genes. XVIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. (PubMed id 10997877)^{3, 4} Nagase T....Ohara O. (2000)
Toward a confocal subcellular atlas of the human proteome. (PubMed id 18029348)³ Barbe L....Andersson-Svahn H. (2008)
The consensus coding sequences of human breast and colorectal cancers. (PubMed id 16959974)⁴ Sjoeblom T.... Velculescu V.E. (2006)
A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration. (PubMed id 16713569)³ Lim J....Zoghbi H.Y. (2006)
Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)³ Strausberg R.L....Marra M.A. (2002)
Hepatitis C virus core protein interacts with 14-3-3 protein and activates the kinase Raf-1. (PubMed id )⁴ Aoki H.... Hino O. (2000)

(in PubMed, OMIM, and NCBI Bookshelf)
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		AND	OR
Aliases
Free Text

Query String		PubMed OMIM NCBI Bookshelf
	(Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 57692	HGNC: 24934	AceView: MAGEE1	Ensembl:ENSG00000198934	euGenes: HUgn57692
ECgene: MAGEE1	H-InvDB: MAGEE1

(According to HUGE)
About This Section

HUGE: KIAA1587

(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
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Name	Description
ATLAS Chromosomes in Cancer entry for MAGEE1	Genetics and Cytogenetics in Oncology and Haematology

Licensable Technologies for MAGEE1
(Available from WIS Yeda, Salk, Tufts)

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(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
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Products for MAGEE1:

TaqMan ® Gene Expression Assays

TaqMan ® Genotyping Assays

Free SNP selection tool

Invitrogen iPath Pathways	Invitrogen BLOCK-iT™ RNAi
Invitrogen Antibodies	Invitrogen Assays
Invitrogen Clones	Invitrogen Q-PCR Products
Invitrogen Human Recombinant Kinases	Invitrogen Custom Antibody and Peptide Service
Invitrogen Proteins / Assays / Screening Services	Search Invitrogen catalog for MAGEE1-related products

	Millipore Custom Antibody & Bulk Services
	Millipore Preclinical / Clinical Development Services
	Millipore Immunoassay Services
	Millipore Target Screening & Profiling Services

	Predesigned and custom siRNAs for MAGEE1		Antibodies for MAGEE1
	Explore super-pooled esiRNAs		Browse proteins at Sigma-Aldrich
	Lentivirus-delivered shRNAs for MAGEE1		Browse small molecules at Sigma-Aldrich
	"Your Favorite Gene" Pathways		feedback

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	Cell Culture Products		ELISAs
	Flow Cytometry Kits		Protease Activity Assays & Reagents
	Cell Selection/Detection Kits & Reagents		ELISA/Assay Development Kits & Reagents
	Multiplex/Array Assay Kits & Reagents		ELISpot Kits & Development Modules
	Recombinant & Natural Proteins

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MAGEE1 Gene

protein-coding GIFtS: 53

Aliases &
Descriptions
for MAGEE1

Summaries
for MAGEE1

Genomic Location
for MAGEE1

Proteins
for MAGEE1

Protein Domains/
Families
for MAGEE1

Gene Function
for MAGEE1

Pathways & Interactions
for MAGEE1

Drugs & Compounds
for MAGEE1

Transcripts
for MAGEE1

Expression
for MAGEE1

Orthologs
for MAGEE1

Paralogs
for MAGEE1

SNPs/Variants
for MAGEE1

Disorders & Mutations
for MAGEE1

Medical News
for MAGEE1

Publications
for MAGEE1

Search for MAGEE1

Genome Databases showing MAGEE1

Other Databases showing MAGEE1

Specialized Databases showing MAGEE1

Licensable Technologies
for MAGEE1

Services
for MAGEE1

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

MAGEE1 Gene

protein-coding GIFtS: 53

Aliases & Descriptionsfor MAGEE1

Summariesfor MAGEE1

Genomic Locationfor MAGEE1

Proteinsfor MAGEE1

Protein Domains/Familiesfor MAGEE1

Gene Functionfor MAGEE1

Pathways & Interactionsfor MAGEE1

Drugs & Compoundsfor MAGEE1

Transcriptsfor MAGEE1

Expression for MAGEE1

Orthologsfor MAGEE1

Paralogsfor MAGEE1

SNPs/Variantsfor MAGEE1

Disorders & Mutationsfor MAGEE1

Medical Newsfor MAGEE1

Publicationsfor MAGEE1

Search for MAGEE1

Genome Databases showing MAGEE1

Other Databases showing MAGEE1

Specialized Databases showing MAGEE1

Licensable Technologiesfor MAGEE1

Servicesfor MAGEE1

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

Aliases &
Descriptions
for MAGEE1

Summaries
for MAGEE1

Genomic Location
for MAGEE1

Proteins
for MAGEE1

Protein Domains/
Families
for MAGEE1

Gene Function
for MAGEE1

Pathways & Interactions
for MAGEE1

Drugs & Compounds
for MAGEE1

Transcripts
for MAGEE1

Expression
for MAGEE1

Orthologs
for MAGEE1

Paralogs
for MAGEE1

SNPs/Variants
for MAGEE1

Disorders & Mutations
for MAGEE1

Medical News
for MAGEE1

Publications
for MAGEE1

Licensable Technologies
for MAGEE1

Services
for MAGEE1