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MAGED4B Gene

protein-coding GIFtS: 43

GC0XM051822

melanoma antigen family D, 4B
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc , and/or ⁷Ensembl, ⁸miRBase)
About This Section

Aliases
KIAA1859 ^², ³
MAGED4A ^³
MAGEE1 ^³
MGC3210 ^²
MGC88639 ^²
OTTHUMP00000023307 ^²

Descriptions
MAGE-D4 antigen ^³
MAGE-E1 antigen ^³
melanoma antigen family D, 4B ^²

External Ids
HGNC: 22880¹
Entrez Gene: 81557²
UniProtKB: Q96JG8³
Ensembl: ENSG00000187243⁷

Search outside databases for aliases for MAGED4B gene

Previous GC identifer: GC0XU900705

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for MAGED4B:

This gene is a member of the MAGED gene family. It is expressed only in brain and ovary among

normal tissues, and two transcript variants of this gene are specifically expressed in glioma

cells among cancer cells. This gene and the other MAGED genes are clustered on chromosome Xp11.

Multiple alternatively spliced transcript variants have been found for this gene, however, the

full-length nature of some variants has not been defined. [provided by RefSeq]

Gene Wiki entry for MAGED4B

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:

UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: Xp11 Ensembl cytogenetic band: Xp11.22 HGNC cytogenetic band: Xp11

MAGED4B Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome X GeneLoc Exon Structure

GeneLoc location for GC0XM051822: (about GC identifiers)

Start:	51,821,663 bp from pter
End:	51,829,108 bp from pter
Size:	7,446 bases
Orientation:	minus strand

RefSeq DNA sequence:

NC_000023.9 NT_011638.12

(According to ¹UniProtKB, and/or Ensembl, Phosphorylation sites according to ²Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: MAGD4_HUMAN, Q96JG8 (See protein sequence)

Recommended Name: Melanoma-associated antigen D4

Size: 741 amino acids; 81378 Da

Sequence caution: Sequence=AAL50032.1; Type=Frameshift; Positions=186, 219;

Secondary accessions: A8K093 Q5HYN6 Q8WXW4 Q9BQ84 Q9BVH1 Q9BYH3 Q9BYH4 Q9H217

Alternative splicing: 4 isoforms: Q96JG8-1 Q96JG8-2 Q96JG8-3 Q96JG8-4

Post-translational modifications:

View phosphorylation sites using PhosphoSite²

REFSEQ proteins (3 alternative transcripts):

NP_110428.2 NP_803879.1 NP_803881.1

ENSEMBL proteins:

ENSP00000353252 ENSP00000335385

Human Recombinant Proteins

	Browse Drug Discovery Central at Invitrogen for human recombinant proteins
	Browse Purified and Recombinant Proteins at Millipore
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	Browse R&D Systems for human recombinant proteins
	Browse recombinant and purified proteins available from Enzo Life Sciences
	Browse Abcam for Recombinant Proteins
	Human Recombinant Proteins from Abnova (MAGED4B)

Browse Origene for full length recombinant human proteins expressed in human HEK293 cells

Antibodies for MAGED4B:

	Browse Antibodies Central at Invitrogen
	Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
	Sigma-Aldrich Antibodies for MAGED4B
	Browse R&D Systems for Antibodies
	Search for antibodies at Abcam and the World's Antibody Gateway (free search engine of over 150 antibody companies)
	Browse Abnova for Monoclonal and Polyclonal Antibodies
	Search Novus for antibodies for MAGED4B

Assays for MAGED4B:

	Browse Invitrogen for biochemical assays
	Browse Kits and Assays available from Millipore
	Browse R&D Systems for biochemical assays
	Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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Graphical View of Domain Structure for InterPro Entry Q96JG8

ProtoNet protein and cluster: Q96JG8

1 Blocks protein family: IPB002190 MAGE protein

UniProtKB/Swiss-Prot: MAGD4_HUMAN, Q96JG8

Similarity: Contains 1 MAGE domain

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

Inhib. RNA:		Invitrogen RNAi Products for gene knock-down (MAGED4B)
		Browse for Gene Knock-down Tools from Millipore
		Browse Abnova for Chimera RNAi Products

OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 3): NM_177535

Applied Biosystems Silencer® siRNAs for MAGED4B

Sigma-Aldrich siRNA for MAGED4B

Sigma-Aldrich shRNA for MAGED4B

Explore Sigma-Aldrich super-pooled esiRNAs

Clones:		Browse Clone Ranger at Invitrogen for clones
		Browse Clones for the Expression of Recombinant Proteins Available from Millipore

OriGene GFP tagged cDNA clones in CMV expression vector (see all 3): NM_030801
Myc/DDK tagged cDNA clones in CMV expression vector (see all 3): NM_030801
untagged cDNA clones in CMV expression vector (see all 4): AK094541

Primers:

Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers

OriGene genome-wide validated SYBR primer pairs: NM_177535

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to ¹UniProtKB, ²MINT, and/or ³STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences

Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for MAGED4B

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib. RNA:		Invitrogen RNAi Products for gene knock-down (MAGED4B)
		Browse for Gene Knock-down Tools from Millipore
		Browse Abnova for Chimera RNAi Products

OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 3): NM_177535

Sigma-Aldrich siRNA for MAGED4B

Sigma-Aldrich shRNA for MAGED4B

Explore Sigma-Aldrich super-pooled esiRNAs

Applied Biosystems Silencer® siRNAs:

NM_030801 NM_177535 NM_177537

REFSEQ mRNAs for MAGED4B gene (3 alternative transcripts):

NM_030801.2 NM_177535.1 NM_177537.1

Applied Biosystems TaqMan ® Gene Expression Assays:

NM_030801 NM_177535 NM_177537

               OriGene GFP tagged cDNA clones in CMV expression vector (see all 3): NM_030801
                                 Myc/DDK tagged cDNA clones in CMV expression vector (see all 3): NM_030801
                                 untagged cDNA clones in CMV expression vector (see all 4): AK094541

Additional cDNA sequence:

AB040527.1 AB040528.1 AB040529.1 AB058762.2 AF320908.1 AF329733.1 AK098830.1 AK308365.1

AL136582.1 BC001207.1 BC063838.1 BC081566.1 CR604784.1

24/27 DOTS entries (see all 27 ):

DT.100681454 DT.75111770 DT.100816309 DT.92430356 DT.100665895 DT.100816300 DT.100816303 DT.40107206

DT.100816302 DT.121302806 DT.121302697 DT.100816308 DT.121302770 DT.100816294 DT.121318310 DT.97763826

DT.102825067 DT.100799336 DT.100840251 DT.121302692 DT.121302698 DT.121318314 DT.95301976 DT.100808063

24/191 AceView cDNA sequences (see all 191 ):

AK098830 BI822544 BU623966 AB058762 AI467910 BX431256 AI271662 BV174918

BQ932562 AW071935 CR615914 NM_177537 AA232504 AI142536 BU517266 BQ898081

BG818476 AB040528 AA779042 BM984725 CF131382 BM998073 AB040527 AW068986

Unigene Cluster for MAGED4B:

Melanoma antigen family D, 4B

Hs.522650 [show with all ESTs]

Unigene Representative Sequence: AB040529

GeneLoc Exon Structure

2 Ensembl transcripts including schematic representations:

ENST00000360134 ENST00000335504

(Experimental results according to ¹GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to ²GeneAnnot , ³GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

MAGED4B expression in normal and diseased human tissues

Applied Biosystems TaqMan ® Gene Expression Assays for MAGED4B

¹ / ²

7 probe-sets matching MAGED4B gene

Affymetrix probe-set	Array	# genes	Sensitivity	Specificity	Correlation	Length	Gb_Accession	Consensus	Uniqueness	Score	Rank
Affymetrix probe-set	Array	GeneAnnot data			GeneNote data		GeneTide data
54896_g_at²	U95-B	2	1.00	0.50	0.97	1.35	--	--	--	--	--
54894_at²	U95-B	2	1.00	0.50	0.96	0.75	--	--	--	--	--
64928_at²	U95-C	2	0.56	0.50	0.98	0.92	--	--	--	--	--
223313_s_at²	U133-B	2	1.00	0.50	--	--	--	--	--	--	--
221261_x_at²	U133-A	2	1.00	0.50	--	--	--	--	--	--	--
223313_s_at²	U133Plus2	2	1.00	0.50	--	--	--	--	--	--	--
221261_x_at²	U133Plus2	2	1.00	0.50	--	--	--	--	--	--	--

GeneDecks MAGED4B for binary patterns associated with the probe-sets selected above

About GeneDecksing

About this table

Data from

(Publications) and GNF BioGPS
About these images
About these images

CGAP SAGE TAG: --

SOURCE GeneReport for Unigene cluster: Hs.522650
UniProtKB/Swiss-Prot: MAGD4_HUMAN, Q96JG8
Tissue specificity: Expressed only in brain and ovary among normal tissues. Isoform 1 and isoform 2
are specifically expressed in glioma cells among cancer cells. Detected in some renal cell
carcinoma samples

(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD and/or ⁵MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section

ENSEMBL Gene Tree for MAGED4B

(Paralogs according to ¹HomoloGene
and ²Ensembl, Pseudogenes according to ³Pseudogene.org)
About This Section

Paralogs for MAGED4B gene

MAGED4¹ ² LOC100132401² MAGED1² MAGED2² TRO²

(According to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section

(in which this Gene is Involved, According to OMIM, UniProtKB, Novoseek, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 300765

10 Novoseek disease relationships for MAGED4B gene

Disease	Score	Articles	PubMed IDs for Articles with Shared Sentences (# sentences)
melanoma	72.91	33	11756773 (5), 15223957 (5), 15946236 (2), 7750125 (1) (see all 18)
metastatic melanoma	48.99	1	9185691 (1)
glioblastoma	48.67	2	15256472 (1), 8509230 (1)
astrocytoma	42.91	1	12494904 (1)
malignant glioma	37.24	1	15256472 (1)
glioma	36.66	3	9154761 (2), 15256472 (1)
metastasis	27.99	2	15305155 (1), 9650552 (1)
tumors	25.41	23	16225959 (4), 9154761 (1), 8524854 (1), 9176405 (1) (see all 6)
brain tumors	20.89	2	9154761 (1)
cancer	6.73	3	12494904 (1), 16225959 (1), 17803450 (1)

About this table

(Possibly Related Articles in Doctor's Guide)
About This Section

(in PubMed. Associations of this gene to articles via ¹Novoseek, ²HGNC, ³Entrez Gene, ⁴UniProtKB/Swiss-Prot, ⁵UniProtKB/TrEMBL, ⁶GAD, and/or ⁷PharmGKB)
About This Section

10/43 PubMed articles for MAGED4B gene (see all 43 ):

Structural characterization and chromosomal localization of the MAGE- E1 gene. (PubMed id 11602350)^{1, 3, 4} Kawano Y....Ikenaka K. (2001)
Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)^{3, 4} Ota T....Sugano S. (2004)
The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)^{3, 4} Gerhard D.S....Malek J. (2004)
Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones. (PubMed id 12168954)^{3, 4} Nakajima D.... Nagase T. (2002)
Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs. (PubMed id 11230166)^{3, 4} Wiemann S.... Poustka A. (2001)
Prediction of the coding sequences of unidentified human genes. XX. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. (PubMed id 11347906)^{3, 4} Nagase T....Ohara O. (2001)
MAGE-E1, a new member of the melanoma-associated antigen gene family and its expression in human glioma. (PubMed id 11406556)^{3, 4} Sasaki M.... Ikenaka K. (2001)
Expression of MAGE-D4, a novel MAGE family antigen, is correlated with tumor-cell proliferation of non-small cell lung cancer. (PubMed id 16225959)^{1, 3} Ito S....Tanaka F. (2006)
The DNA sequence of the human X chromosome. (PubMed id 15772651)⁴ Ross M.T.... Bentley D.R. (2005)
MAGED4-expression in renal cell carcinoma and identification of an HLA-A*25-restricted MHC class I ligand from solid tumor tissue. (PubMed id 16082191)⁴ Kraemer B.F....Stevanovic S. (2005)

(in PubMed, OMIM, and NCBI Bookshelf)
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		AND	OR
Aliases
Free Text

Query String		PubMed OMIM NCBI Bookshelf
	(Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
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Entrez Gene: 81557	HGNC: 22880	AceView: MAGED4	Ensembl:ENSG00000187243	euGenes: HUgn81557
ECgene: MAGED4B	H-InvDB: MAGED4B

(According to HUGE)
About This Section

HUGE: KIAA1859

(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
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Licensable Technologies for MAGED4B
(Available from WIS Yeda, Salk, Tufts)

About This Section

(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
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Products for MAGED4B:

TaqMan ® Gene Expression Assays

TaqMan ® Genotyping Assays

Free SNP selection tool

Invitrogen iPath Pathways	Invitrogen BLOCK-iT™ RNAi
Invitrogen Antibodies	Invitrogen Assays
Invitrogen Clones	Invitrogen Q-PCR Products
Invitrogen Human Recombinant Kinases	Invitrogen Custom Antibody and Peptide Service
Invitrogen Proteins / Assays / Screening Services	Search Invitrogen catalog for MAGED4B-related products

	Millipore Custom Antibody & Bulk Services
	Millipore Preclinical / Clinical Development Services
	Millipore Immunoassay Services
	Millipore Target Screening & Profiling Services

	Predesigned and custom siRNAs for MAGED4B		Antibodies for MAGED4B
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	Multiplex/Array Assay Kits & Reagents		ELISpot Kits & Development Modules
	Recombinant & Natural Proteins

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MAGED4B Gene

protein-coding GIFtS: 43

Aliases &
Descriptions
for MAGED4B

Summaries
for MAGED4B

Genomic Location
for MAGED4B

Proteins
for MAGED4B

Protein Domains/
Families
for MAGED4B

Gene Function
for MAGED4B

Pathways & Interactions
for MAGED4B

Drugs & Compounds
for MAGED4B

Transcripts
for MAGED4B

Expression
for MAGED4B

Orthologs
for MAGED4B

Paralogs
for MAGED4B

SNPs/Variants
for MAGED4B

Disorders & Mutations
for MAGED4B

Medical News
for MAGED4B

Publications
for MAGED4B

Search for MAGED4B

Genome Databases showing MAGED4B

Other Databases showing MAGED4B

Specialized Databases showing MAGED4B

Licensable Technologies
for MAGED4B

Services
for MAGED4B

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

MAGED4B Gene

protein-coding GIFtS: 43

Aliases & Descriptionsfor MAGED4B

Summariesfor MAGED4B

Genomic Locationfor MAGED4B

Proteinsfor MAGED4B

Protein Domains/Familiesfor MAGED4B

Gene Functionfor MAGED4B

Pathways & Interactionsfor MAGED4B

Drugs & Compoundsfor MAGED4B

Transcriptsfor MAGED4B

Expression for MAGED4B

Orthologsfor MAGED4B

Paralogsfor MAGED4B

SNPs/Variantsfor MAGED4B

Disorders & Mutationsfor MAGED4B

Medical Newsfor MAGED4B

Publicationsfor MAGED4B

Search for MAGED4B

Genome Databases showing MAGED4B

Other Databases showing MAGED4B

Specialized Databases showing MAGED4B

Licensable Technologiesfor MAGED4B

Servicesfor MAGED4B

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

Aliases &
Descriptions
for MAGED4B

Summaries
for MAGED4B

Genomic Location
for MAGED4B

Proteins
for MAGED4B

Protein Domains/
Families
for MAGED4B

Gene Function
for MAGED4B

Pathways & Interactions
for MAGED4B

Drugs & Compounds
for MAGED4B

Transcripts
for MAGED4B

Expression
for MAGED4B

Orthologs
for MAGED4B

Paralogs
for MAGED4B

SNPs/Variants
for MAGED4B

Disorders & Mutations
for MAGED4B

Medical News
for MAGED4B

Publications
for MAGED4B

Licensable Technologies
for MAGED4B

Services
for MAGED4B