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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

MAGED4 Gene

protein-coding   GIFtS: 43
GCID: GC0XP051927

melanoma antigen family D, 4

 Explore 3 diseases affiliated with
MAGED4 via our new
 Human Malady Compendium 
Biological research products
for MAGED4
    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section

Aliases
Melanoma Antigen Family D, 41 2     MAGE-D4 Antigen2 3
KIAA18591 3 5     MAGE-E1 Antigen2 3
MAGEE12 3 5     MGC32101
MAGE-E11 2     Melanoma-Associated Antigen D42
MAGE11 2     MAGED4A3

External Ids:    HGNC: 237931   Entrez Gene: 7282392   Ensembl: ENSG000001545457   OMIM: 3007025   UniProtKB: Q96JG83   

Export aliases for MAGED4 gene to outside databases

Previous GC identifers: GC0XP049889 GC0XP050579 GC0XM050721 GC0XM051637 GC0XM051821 GC0XP051949 GC0XP051952


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: MAGD4_HUMAN, Q96JG8
Function: May enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases.
Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate
complex




(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000023.10  NC_018934.1  NT_011638.13  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the MAGED4 gene promoter:
         RFX1   STAT5A   NF-AT   HNF-4alpha1   Meis-1b   IRF-7A   ZID   Meis-1a   NF-AT1   Meis-1   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidMAGED4 promoter sequence
   Search SABiosciences Chromatin IP Primers for MAGED4

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat MAGED4


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: Xp11.22   Ensembl cytogenetic band:  Xp11.22   HGNC cytogenetic band: Xp11

MAGED4 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
MAGED4 gene location

GeneLoc information about chromosome X         GeneLoc Exon Structure

GeneLoc location for GC0XP051927:  view genomic region     (about GC identifiers)

Start:
51,927,919 bp from pter      End:
51,935,364 bp from pter
Size:
7,446 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: MAGD4_HUMAN, Q96JG8 (See protein sequence)
Recommended Name: Melanoma-associated antigen D4  
Size: 741 amino acids; 81378 Da
Subunit: Interacts with TRIM27
Sequence caution: Sequence=AAH01207.1; Type=Erroneous initiation; Sequence=AAL50032.1; Type=Frameshift; Positions=186,
219; Sequence=BAB47488.2; Type=Erroneous initiation;
Secondary accessions: A8K093 Q5HYN6 Q8WXW4 Q9BQ84 Q9BVH1 Q9BYH3 Q9BYH4 Q9H217
Alternative splicing: 4 isoforms:  Q96JG8-1   Q96JG8-2   Q96JG8-3   Q96JG8-4   (No experimental confirmation available)

Explore the universe of human proteins at neXtProt for MAGED4: NX_Q96JG8

Post-translational modifications:

  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_Q96JG8

  • MAGED4 Protein expression data from MOPED and PaxDb:    About this image 
    Estimated protein expression log10 (pmol).

    REFSEQ proteins (4 alternative transcripts): 
    NP_001092270.1  NP_001258990.1  NP_001258991.1  NP_001258992.1  

    ENSEMBL proteins: 
     ENSP00000364777   ENSP00000399147  

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    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    MAGED4 for domains           About GeneDecksing

    1 InterPro domain/family:
     IPR002190 MAGE

    Graphical View of Domain Structure for InterPro Entry Q96JG8

    ProtoNet protein and cluster: Q96JG8

    1 Blocks protein family: IPB002190 MAGE protein

    UniProtKB/Swiss-Prot: MAGD4_HUMAN, Q96JG8
    Similarity: Contains 1 MAGE domain


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section

    Function Summary:

         UniProtKB/Swiss-Prot: MAGD4_HUMAN, Q96JG8
    Function: May enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases.
    Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate
    complex

    miRNA
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    hsa-miR-411* hsa-miR-141 hsa-miR-379* hsa-miR-891b hsa-miR-200a hsa-miR-450b-5p
    SwitchGear 3'UTR luciferase reporter plasmidMAGED4 3' UTR sequence
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    1 GenomeRNAi human phenotype for MAGED4:
     Synthetic lethal with Ras 


    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section



    Interactions:

        Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for MAGED4

    STRING Interaction Network Preview (showing 1 interactants - click image to see more details)

    5 Interacting proteins for MAGED4 (Q96JG82, 3 ENSP000003647774) via UniProtKB, MINT, STRING, and/or I2D
    InteractantInteraction Details
    GeneCardExternal ID(s)
    MAPK8IP2Q133872, 3MINT-8262521 I2D: score=2 
    UBCP0CG483, ENSP000003448184I2D: score=1 STRING: ENSP00000344818
    PHYHIPQ925613I2D: score=4 
    CHMP243863I2D: score=1 
    RAE1P784063I2D: score=1 
    About this table

    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section
    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for MAGED4
    Search CenterWatch for drugs/clinical trials and news about MAGED4 / MAGD4 

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
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    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for MAGED4 gene (4 alternative transcripts): 
    NM_001098800.2  NM_001272061.1  NM_001272062.1  NM_001272063.1  

    Unigene Cluster for MAGED4:

    Melanoma antigen family D, 4
    Hs.571729  [show with all ESTs]
    Unigene Representative Sequence: BC001207
    12 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000471932 ENST00000459685 ENST00000488430 ENST00000375626 ENST00000477634
    ENST00000498483 ENST00000467526 ENST00000474812 ENST00000479281 ENST00000497416
    ENST00000497348 ENST00000416960(uc004dpz.3 uc004dpx.3 uc004dpw.3 uc004dpy.3 uc004dqa.3)


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    6 QIAGEN miScript miRNA Assays for microRNAs that regulate MAGED4:
    hsa-miR-411* hsa-miR-141 hsa-miR-379* hsa-miR-891b hsa-miR-200a hsa-miR-450b-5p
    SwitchGear 3'UTR luciferase reporter plasmidMAGED4 3' UTR sequence
    Inhib. RNA
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    OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for MAGED4
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    Clone
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      QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat MAGED4
      QIAGEN QuantiFast Probe-based Assays in human, mouse, rat MAGED4

    Additional cDNA sequence: 

    AB040527.1 AB040528.1 AB058762.2 AF320908.1 AF329733.1 AK098830.1 AL136582.1 BC001207.1 
    BC063838.1 BC081566.1 

    20 DOTS entries:

    DT.100681454  DT.75111770  DT.100816309  DT.92430356  DT.100816300  DT.100665895  DT.100808063  DT.100816294 
    DT.100816303  DT.121302770  DT.121302806  DT.121318307  DT.121318314  DT.95301976  DT.100816302  DT.100816308 
    DT.102825067  DT.121302697  DT.121318299  DT.40107206 

    GeneLoc Exon Structure


    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    MAGED4 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: --

    Microarray
    RNAseq (Illumina Body Map)
    (100×FPKM)½
    SAGE (Serial Analysis of Gene Expression)

    About this image
    See MAGED4 Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for MAGED4

    SOURCE GeneReport for Unigene cluster: Hs.571729

    UniProtKB/Swiss-Prot: MAGD4_HUMAN, Q96JG8
    Tissue specificity: Expressed only in brain and ovary among normal tissues. Isoform 1 and isoform 2 are specifically
    expressed in glioma cells among cancer cells. Detected in some renal cell carcinoma samples

        SABiosciences Custom PCR Arrays for MAGED4
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    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for MAGED4

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of animals.

    Orthologs for MAGED4 gene from 3/15 species (see all 15)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    lizard
    (Anolis carolinensis)
    Reptilia --
    --
    37(a)
    1 → many
    1(205669616-205678308)
    zebrafish
    (Danio rerio)
    Actinopterygii ndnl26
    necdin-like 2
    34(a)
    1 → many
    23(13012414-13025121)
    fruit fly
    (Drosophila melanogaster)
    Insecta MAGE6
    MAGE
    23(a)
    1 → many
    3R(2979960-2980898)


    ENSEMBL Gene Tree for MAGED4 (if available)
    TreeFam Gene Tree for MAGED4 (if available) 

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for MAGED4 gene
    MAGED4B1 2  MAGEC22  MAGEA9B2  MAGEB52  MAGEB162  MAGEB42  MAGEB12  MAGEC12  
    MAGEA82  MAGED22  MAGEA122  MAGEA92  MAGEB172  MAGEB102  MAGEA62  MAGEE12  
    MAGED12  MAGEA22  MAGEC32  MAGEA32  NDN2  MAGEB22  MAGEB32  MAGEE22  
    MAGEA42  MAGEA12  MAGEA102  MAGEB182  MAGEA2B2  MAGEB62  MAGEL22  TRO2  
    MAGEA112  NDNL22  MAGEF12  
    18/23 SIMAP similar genes for MAGED4 using alignment to 1 protein entry:     MAGD4_HUMAN(see all similar genes):
    MAGED4B    MAGEH1    TRO    MAGEA8    MAGEB2    MAGEA3
    MAGEA12    MAGEA6    MAGEF1    MAGED2    MAGEB3    MAGEC4
    NDNL2    MAGEB4    MAGEA4    MAGEA1    MAGEA9    MAGEA9B

    MAGED4 for paralogs           About GeneDecksing



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    3 NCBI SNPs in MAGED4 are shown     About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr X posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs2016532311,2
    --51929213(+) CCAGGC/TCTTCG 2 G syn10--------
    rs2009210011,2
    --51931766(+) CCCACA/GTAGAA 2 -- int1 us2k10--------
    rs2003668011,2
    --51934214(+) GGGAGA/CGCCTC 2 -- int1 ds50010--------

    HapMap Linkage Disequilibrium report for MAGED4 (51927919 - 51935364 bp)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
          Database of Genomic Variants (DGV) variations for MAGED4: --

    SABiosciences Cancer Mutation PCR Assays
    Search QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing MAGED4
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    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    MAGED4 for disorders           About GeneDecksing

    OMIM gene information: 300702    OMIM disorders: --

    3 diseases for MAGED4:    About MalaCards
    melanoma    renal cell carcinoma    carcinoma


    Export disorders for MAGED4 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for MAGED4 gene, integrated from 9 sources (see all 17):
    (articles sorted by number of sources associating them with MAGED4)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. MAGE-E1, a new member of the melanoma-associated antigen gene family and its expression in human glioma. (PubMed id 11406556)1, 2, 3 Sasaki M.... Ikenaka K. (2001)
    2. Structural characterization and chromosomal localization of the MAGE- E1 gene. (PubMed id 11602350)1, 2 Kawano Y....Ikenaka K. (2001)
    3. The proteomic analysis of endogenous FAT10 substrates identifies p62/SQSTM1 as a substrate of FAT10ylation. (PubMed id 22797925)1 Aichem A....Groettrup M. (2012)
    4. Methods for quantification of in vivo changes in prote in ubiquitination following proteasome and deubiquitinase inhibition. (PubMed id 22505724)1 Udeshi N.D....Carr S.A. (2012)
    5. Ubiquitin-specific peptidase 9, X-linked (USP9X) modul ates activity of mammalian target of rapamycin (mTOR). (PubMed id 22544753)1 Agrawal P....Hughes R.E. (2012)
    6. MAGE-RING protein complexes comprise a family of E3 u biquitin ligases. (PubMed id 20864041)2 Doyle J.M....Potts P.R. (2010)
    7. Expression of MAGE-D4, a novel MAGE family antigen, is correlated with tumor-cell proliferation of non-small cell lung cancer. (PubMed id 16225959)1 Ito S....Tanaka F. (2006)
    8. The DNA sequence of the human X chromosome. (PubMed id 15772651)2 Ross M.T.... Bentley D.R. (2005)
    9. MAGED4-expression in renal cell carcinoma and identification of an HLA-A*25-restricted MHC class I ligand from solid tumor tissue. (PubMed id 16082191)2 Kraemer B.F....Stevanovic S. (2005)
    10. Large-scale analysis of the human ubiquitin-related p roteome. (PubMed id 16196087)1 Matsumoto M....Nakayama K.I. (2005)

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section
     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 728239 HGNC: 23793 Ensembl:ENSG00000154545 euGenes: HUgn728239 ECgene: MAGED4
    H-InvDB: MAGED4

    (According to HUGE)
    About This Section
    HUGE: KIAA1859

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for MAGED4 Pharmacogenomics, SNPs, Pathways
    ATLAS Chromosomes in Cancer entry for MAGED4 Genetics and Cytogenetics in Oncology and Haematology

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section
    Patent Information for MAGED4 gene:
    Search GeneIP for patents involving MAGED4

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or Enzo Life Sciences),
    In Situ Hybridization Assays from
    Advanced Cell Diagnostics
    About This Section

     
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    GeneCards Homepage - Last full update: 19 Mar 2013 - Incrementals: 21 Mar 2013 , 15 Apr 2013 , 26 Apr 2013

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    VWF
    (GIFTS: 73)
    von Willebrand factor
    GIFtS Group
    The GeneCards human gene database gene index: 1 3 5 6 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 


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