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MAGED2 Gene

protein-coding   GIFtS: 62

GC0XP054850
melanoma antigen family D, 2
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases
11B6 1, 2, 3
BCG-1 3
BCG1 1, 2, 3
HCA10 1, 2
JCL-1 1, 2
MAGE-D 3
MAGE-D2 1, 2
MAGED 1, 2, 5
MGC8386 1, 2
OTTHUMP00000023381 2
OTTHUMP00000023382 2
Descriptions
Breast cancer-associated gene 1 protein 3
Hepatocellular carcinoma-associated protein JCL-1 3
MAGE-D2 antigen 3
breast cancer associated gene 1 2
hepatocellular carcinoma associated protein 2
hepatocellular carcinoma-associated protein HCA10 2
melanoma antigen family D, 2 2
melanoma-associated antigen D2 2
External Ids
HGNC: 163531
Entrez Gene: 109162
UniProtKB: Q9UNF13
Ensembl: ENSG000001023167
Search outside databases for aliases for MAGED2 gene

Previous GC identifers: GC0XP051686 GC0XP052772 GC0XP053439 GC0XP053800 GC0XP054717

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for MAGED2:
This gene is a member of the MAGED gene family. While the MAGEA and MAGEB genes are silent in
normal tissues with the exception of testis and placenta, the MAGED genes are expressed
ubiquitously. The MAGED genes are clustered on chromosome Xp11. This gene is located in Xp11.2, a
hot spot for X-linked mental retardation (XLMR). Multiple alternatively spliced transcript
variants have been found for this gene, however, the full length nature of some variants has not
been defined. [provided by RefSeq]

Gene Wiki entry for MAGED2
(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the MAGED2 gene  

Entrez Gene cytogenetic band: Xp11.2   Ensembl cytogenetic band:  Xp11.21   HGNC cytogenetic band: Xp11.2

MAGED2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome X         GeneLoc Exon Structure

GeneLoc location for GC0XP054850:     (about GC identifiers)

Start:
54,850,757 bp from pter
End:
54,859,170 bp from pter
Size:
8,414 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000023.9  NT_011630.14  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: MAGD2_HUMAN, Q9UNF1 (See protein sequence)
Recommended Name: Melanoma-associated antigen D2  
Size: 606 amino acids; 64954 Da
Secondary accessions: A6NMX0 O76058 Q5BJF3 Q8NAL6 Q9H218 Q9P0U9 Q9UM52
Alternative splicing: 2 isoforms:  Q9UNF1-1   Q9UNF1-2   

Post-translational modifications:

  • Phosphorylated upon DNA damage, probably by ATM or ATR1
  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins (3 alternative transcripts): 
    NP_055414.2  NP_803182.1  NP_957516.1  


    ENSEMBL proteins: 
    ENSP00000364198 ENSP00000364200 ENSP00000364209 ENSP00000336962 ENSP00000364193 ENSP00000340290 
    ENSP00000218439 ENSP00000379526 ENSP00000364202 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (MAGED2)
    Human Recombinant Proteins from Abnova (MAGED2)
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 


    Antibodies for MAGED2: 
    Browse Antibodies Central at Invitrogen
    Millipore Mono- and Polyclonal Antibodies for the study of MAGED2
    Sigma-Aldrich Antibodies for MAGED2
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (MAGED2), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (MAGED2)
    Novus Biologicals Antibodies for MAGED2

    Assays for MAGED2: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    1 InterPro domain/family:
     IPR002190 MAGE


       GeneDecks  MAGED2 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q9UNF1

    ProtoNet protein and cluster: Q9UNF1

    1 Blocks protein family: IPB002190 MAGE protein

    UniProtKB/Swiss-Prot: MAGD2_HUMAN, Q9UNF1
    Similarity: Contains 1 MAGE domain

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (MAGED2)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (MAGED2)
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 3): NM_014599

                  Applied Biosystems Silencer® siRNAs for MAGED2

                  Sigma-Aldrich siRNA for MAGED2  
                         Sigma-Aldrich shRNA for MAGED2  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for MAGED2
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                   OriGene GFP tagged cDNA clones in CMV expression vector (see all 3): NM_014599
                                     Myc/DDK tagged cDNA clones in CMV expression vector (see all 3): NM_014599
                                     untagged cDNA clones in CMV expression vector (see all 5): AK091003 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_177433

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


     Gene Network CentralTM Interacting Genes and Proteins Network for  MAGED2 


    4 Interacting proteins for MAGED2 (ENSP000003642093 Q9UNF12) via UniProtKB, MINT, and/or STRING
    InteractantInteraction Details
    GeneCardExternal ID(s)
    TP53ENSP000002693053STRING (score=.875)
    MTA1ENSP000003336333STRING (score=.72)
    NAP1L1ENSP000002611823STRING (score=.7)
    --Q5JR012MINT-65781
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for MAGED2

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (MAGED2)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (MAGED2)
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 3): NM_014599

                  Sigma-Aldrich siRNA for MAGED2  
                         Sigma-Aldrich shRNA for MAGED2  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_014599  NM_177433  NM_201222  

    REFSEQ mRNAs for MAGED2 gene (3 alternative transcripts): 

    NM_014599.4   NM_177433.1   NM_201222.1   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_014599  NM_177433  NM_201222  

                   OriGene GFP tagged cDNA clones in CMV expression vector (see all 3): NM_014599
                                     Myc/DDK tagged cDNA clones in CMV expression vector (see all 3): NM_014599
                                     untagged cDNA clones in CMV expression vector (see all 5): AK091003 

    Additional cDNA sequence: 

    AF126181.1 AF128527.1 AF128528.1 AF148815.1 AF320907.1 AJ293618.1 AK092463.1 AK098645.1 
    AK297910.1 AK301599.1 AK301821.1 BC000304.2 BC091503.1 BC107040.1 BX647995.1 CR595215.1 
    CR597524.1 CR605686.1 CR606543.1 CR624805.1 U92544.1 

    24/30 DOTS entries (see all 30 ):

    DT.450043  DT.100042116  DT.92044994  DT.92464652  DT.87079441  DT.92464648  DT.100681955  DT.100848601 
    DT.40288367  DT.100681934  DT.95186940  DT.87079444  DT.95186927  DT.100875514  DT.99983002  DT.100042128 
    DT.100681933  DT.100867368  DT.100681946  DT.102835893  DT.95186923  DT.100033072  DT.100042122  DT.100681971 

    24/1068 AceView cDNA sequences (see all 1068 ):

    BM846856 AA176338 CA448962 AA643888 BE383843 BM694110 CB305571 AI564907 
    CF131211 CA447098 BU556549 AA613956 BM550206 AI306585 NM_201222 T30710 
    BU556568 BM980484 BQ720755 BF689822 BF434364 AI669857 AI369794 CR606543 

    highest scoring ESTs for MAGED2:

    AI934895 AA039316 AA081759 AA101569 AA113973 AA113981 AA115167 AA166897 AA172257 AA176356 

    Unigene Cluster for MAGED2:

    Melanoma antigen family D, 2
    Hs.522665  [show with all ESTs]
    Unigene Representative Sequence: AK092463


    GeneLoc Exon Structure

    5/15 Alternative Splicing Database (ASD) splice patterns (SP) for MAGED2 (see all 15 )

    ExUns: 1a · 1b · 1c ^ 2 ^ 3a · 3b ^ 4 ^ 5 ^ 6a · 6b · 6c ^ 7a · 7b · 7c · 7d · 7e ^ 8a · 8b · 8c · 8d ^ 9 ^ 10 ^ 11a · 11b ^ 12a · 12b ^
    SP1:                                      -                       -     -     -                                   -     -                             -         
    SP2:                    -     -     -     -                                                                                                           -         
    SP3:                                      -                                                                                                           -         
    SP4:                                                                                                                                                  -         
    SP5:                    -     -     -     -           -     -     -     -     -     -                                                                           

    ExUns: 13 ^ 14a · 14b · 14c ^ 15 ^ 16 ^ 17 ^ 18a · 18b
    SP1:              -     -                                 
    SP2:              -     -                                 
    SP3:              -     -                                 
    SP4:              -     -                                 
    SP5:                                                      

    About this scheme

    ECgene alternative splicing isoforms for MAGED2

    9 Ensembl transcripts including schematic representations:
    ENST00000375058  ENST00000375060  ENST00000375068  ENST00000347546  ENST00000375053  ENST00000343474  
    ENST00000218439  ENST00000396224  ENST00000375062  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    MAGED2 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for MAGED2

    1 / 2 / 3

    7 probe-sets matching MAGED2 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

    34860_g_at2, 3 U95-A 1 1.00 1.00 0.98 1.02 Z98046 0.20 1.00 0.72 1

    51719_at2, 3 U95-C 1 1.00 1.00 0.96 1.06 AI934895 1.00 1.00 1.00 1

    34859_at2, 3 U95-A 6 1.00 0.95 0.98 0.92 Z98046 0.20 1.00 0.72 1

    208682_s_at2, 3 U133-A 1 1.00 1.00 -- -- AF126181 0.60 0.75 0.68 1

    213627_at2, 3 U133-A 1 1.00 1.00 -- -- AI924630 0.80 1.00 0.91 1

    208682_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    213627_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    About this table
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: AATGGAAATC

    SOURCE GeneReport for Unigene cluster: Hs.522665

    Expression variation in blood from EXPOLDB for MAGED2

    UniProtKB/Swiss-Prot: MAGD2_HUMAN, Q9UNF1
    Tissue specificity: Widely expressed in normal tissues

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for MAGED2 gene from 5 species
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    MAGED21   -- melanoma antigen family D, 2 91.01(n)
    90.12(a)
    480934  XM_859711.1  XP_864804.1 
    chimpanzee
    (Pan troglodytes)
    MAGED21   -- melanoma antigen family D, 2 98.86(n)
    98.64(a)
    473624  XM_528996.2  XP_528996.2 
    cow
    (Bos taurus)
    MAGED21   -- melanoma antigen family D, 2 90.87(n)
    89.8(a)
    514372  NM_001075665.1  NP_001069133.1 
    rat
    (Rattus norvegicus)
    Maged21   -- melanoma antigen, family D, 2 88.34(n)
    88.12(a)
    113947  NM_080479.2  NP_536727.2 
    mouse
    (Mus musculus)
    Maged21, 5 X5
    melanoma antigen, family D, 21, 5 88.17(n)1
    87.62(a)1
    808841  NM_030700.11  NP_109625.11 
     AF3199765  AI8515745  (see all 15)
    About this table        Species with no ortholog for MAGED2

    ENSEMBL Gene Tree for MAGED2
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for MAGED2 gene
    MAGED4B2  LOC1001324012  MAGED42  MAGED12  TRO2  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/35 NCBI SNPs in MAGED2 are shown (see all 35 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 34)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr X posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs7439741,2
    A,C,F,H,O54851682(-) TACGAT/GCCGGT 3 -- ng51 int18Minor allele frequency- G:0.43EU EA WA NA 780
    rs7439751,2
    A,C,F,H54852020(-) TGCGCA/GATGGT 3 -- int1 ng514Minor allele frequency- G:0.02EU EA WA 398
    rs120149771,2
    C,F,H54854002(+) GAAGAG/CGATGG 3 D/E mis1 ese3 trp34Minor allele frequency- C:0.01EU EA WA 406
    rs66142901,2
    H54853098(+) ACTCAG/TCTGAC 3 Q/H mis1 spl3 ese34Minor allele frequency- T:0.00EU EA WA 418
    rs46125171,2
    H54849244(+) cctagA/Cattct 1 -- ng514Minor allele frequency- C:0.00EU EA WA 418
    rs10430341,2
    A,C,F,H,O54853230(+) GATACG/AAAGGT 3 T/T syn1 ese37Minor allele frequency- A:0.46EU EA WA NA 672
    rs20719321,2
    A,C,F,H54854802(+) TATTCC/TTTGGA 3 S/S syn117Minor allele frequency- T:0.41EU EA WA NA MN 1354
    rs10430311,2
    A,C,F,H54853086(+) GCCTCA/GTCTAC 3 S/S syn1 ese39Minor allele frequency- G:0.37MN NA EU EA WA 771
    rs10430441,2
    A,C,F,H54854065(+) AAGGCC/TCTAAT 3 A/A syn1 ese35Minor allele frequency- T:0.49MN EU EA WA 594
    rs115559271,2
    C,F,H54858462(-) AAATCC/TGCTTC 3 A/A syn1 ese38Minor allele frequency- T:0.05NA EU EA WA 608
    About this table

    HapMap Linkage Disequilibrium images for MAGED2 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 300470

    Human Genome Epidemiology Navigator: MAGED2 (1 document)

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/20 PubMed articles for MAGED2 gene (see all 20 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 10916 HGNC: 16353 AceView: MAGED2 Ensembl:ENSG00000102316 euGenes: HUgn10916
    ECgene: MAGED2 H-InvDB: MAGED2
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

    NameDescription
    ATLAS Chromosomes in Cancer entry for MAGED2 Genetics and Cytogenetics in Oncology and Haematology
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for MAGED2:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



      Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
      Invitrogen Antibodies  Invitrogen Assays
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     Predesigned and custom siRNAs for MAGED2 Antibodies for MAGED2
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for MAGED2 Browse small molecules at Sigma-Aldrich
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