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MAGEB3 Gene

protein-coding GIFtS: 50

GC0XP030158

melanoma antigen family B, 3
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc , and/or ⁷Ensembl, ⁸miRBase)
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Aliases
CT3.5 ^¹, ²
OTTHUMP00000023099 ^²

Descriptions
MAGE-B3 antigen ^³
cancer/testis antigen family 3, member 5 ^²
melanoma antigen family B, 3 ^²
melanoma-associated antigen B3 ^²

External Ids
HGNC: 6810¹
Entrez Gene: 4114²
UniProtKB: O15480³
Ensembl: ENSG00000198798⁷

Search outside databases for aliases for MAGEB3 gene

Previous GC identifers: GC0XP028753 GC0XP028932 GC0XP029450 GC0XP029615 GC0XP030008

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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EntrezGene summary for MAGEB3:

This gene is a MAGE-B subfamily member of the MAGE gene family. MAGE family member proteins direct

the expression of tumor antigens recognized on a human melanoma by autologous cytolytic T

lymphocytes. There are two known clusters of MAGE genes on chromosome X. The members of the MAGE-A

subfamily are located in the Xq28 region, while the members of the MAGE-B subfamily are clustered

in the Xp21 region. [provided by RefSeq]

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
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Genomic View:

UCSC Golden Path with GeneCards custom track

Transcription factor binding sites upstream to the MAGEB3 gene

Entrez Gene cytogenetic band: Xp21.3 Ensembl cytogenetic band: Xp21.2 HGNC cytogenetic band: Xp21.3

MAGEB3 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome X GeneLoc Exon Structure

GeneLoc location for GC0XP030158: (about GC identifiers)

Start:	30,158,474 bp from pter
End:	30,165,528 bp from pter
Size:	7,055 bases
Orientation:	plus strand

RefSeq DNA sequence:

NC_000023.9 NT_011757.15

(According to ¹UniProtKB, and/or Ensembl, Phosphorylation sites according to ²Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: MAGB3_HUMAN, O15480 (See protein sequence)

Recommended Name: Melanoma-associated antigen B3

Size: 346 amino acids; 39211 Da

Secondary accessions: A0AVE4 B3KQ52 O75861

REFSEQ proteins: NP_002356.2

ENSEMBL proteins:

ENSP00000368271 ENSP00000355198

Human Recombinant Proteins

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	Recombinant Proteins from Abcam (MAGEB3)
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Browse Origene for full length recombinant human proteins expressed in human HEK293 cells

Antibodies for MAGEB3:

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	Antibodies from Abcam (MAGEB3), each with their Abpromise^SM
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Assays for MAGEB3:

	Browse Invitrogen for biochemical assays
	Browse Kits and Assays available from Millipore
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	Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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Graphical View of Domain Structure for InterPro Entry O15480

ProtoNet protein and cluster: O15480

1 Blocks protein family: IPB002190 MAGE protein

UniProtKB/Swiss-Prot: MAGB3_HUMAN, O15480

Similarity: Contains 1 MAGE domain

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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Inhib. RNA:		Invitrogen RNAi Products for gene knock-down (MAGEB3)
		Browse for Gene Knock-down Tools from Millipore
		Browse Abnova for Chimera RNAi Products

OriGene 29mer shRNA kit in GFP-retroviral vector: NM_002365

Applied Biosystems Silencer® siRNAs for MAGEB3

Sigma-Aldrich siRNA for MAGEB3

Sigma-Aldrich shRNA for MAGEB3

Explore Sigma-Aldrich super-pooled esiRNAs

Clones:		Invitrogen Clones for MAGEB3
		Browse Clones for the Expression of Recombinant Proteins Available from Millipore

OriGene GFP tagged cDNA clone in CMV expression vector: NM_002365
Myc/DDK tagged cDNA clone in CMV expression vector: NM_002365
untagged cDNA clone in CMV expression vector: NM_002365

Primers:

Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers

OriGene genome-wide validated SYBR primer pairs: NM_002365

Genatlas biochemistry entry for MAGEB3:

melanoma antigen,family B,member 3,expressed in normal testis and in testicular tumor

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to ¹UniProtKB, ²MINT, and/or ³STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
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Browse drugs & compounds from Enzo Life Sciences

Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for MAGEB3

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
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Inhib. RNA:		Invitrogen RNAi Products for gene knock-down (MAGEB3)
		Browse for Gene Knock-down Tools from Millipore
		Browse Abnova for Chimera RNAi Products

OriGene 29mer shRNA kit in GFP-retroviral vector: NM_002365

Sigma-Aldrich siRNA for MAGEB3

Sigma-Aldrich shRNA for MAGEB3

Explore Sigma-Aldrich super-pooled esiRNAs

Applied Biosystems Silencer® siRNAs:

NM_002365

REFSEQ mRNAs for MAGEB3 gene:

NM_002365.3

Applied Biosystems TaqMan ® Gene Expression Assays:

NM_002365

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_002365
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_002365
                                 untagged cDNA clone in CMV expression vector: NM_002365

Additional cDNA sequence:

AK057441.1 BC069784.1 BC074756.2 BC126320.1 BC126322.1

1 DOTS entry:

DT.106558

24/61 AceView cDNA sequences (see all 61 ):

CR618278 NM_002365 CR593708 CR618499 CR621494 CR590008 CR611588 BI520298

BX107939 BM471242 BI831893 AL525285 BM473754 AL562631 BQ773446 BU175305

BU188463 AL525917 CA428956 BX329175 AA815379 BC026071 AK057441 BC069784

highest scoring ESTs for MAGEB3:

AA934059 AA936574 AK057441 BC069784 BX113848 BC074756 NM_002365 BC126320 BC126322 BP326186

Unigene Cluster for MAGEB3:

Melanoma antigen family B, 3

Hs.113290 [show with all ESTs]

Unigene Representative Sequence: NM_002365

GeneLoc Exon Structure

2 Ensembl transcripts including schematic representations:

ENST00000378986 ENST00000361644

(Experimental results according to ¹GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to ²GeneAnnot , ³GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
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MAGEB3 expression in normal and diseased human tissues

Applied Biosystems TaqMan ® Gene Expression Assays for MAGEB3

¹ / ² / ³

3 probe-sets matching MAGEB3 gene

Affymetrix probe-set	Array	# genes	Sensitivity	Specificity	Correlation	Length	Gb_Accession	Consensus	Uniqueness	Score	Rank
Affymetrix probe-set	Array	GeneAnnot data			GeneNote data		GeneTide data
32476_at²^{, 3}	U95-A	1	1.00	1.00	1.00	1.00	U93163	0.20	0.33	0.27	1
207579_at²^{, 3}	U133-A	1	1.00	1.00	--	--	NM_002365	0.60	1.00	0.82	1
207579_at²	U133Plus2	1	1.00	1.00	--	--	--	--	--	--	--

About this table

Data from

(Publications) and GNF BioGPS
About these images
About these images

CGAP SAGE TAG: GGCAGAAAGT

SOURCE GeneReport for Unigene cluster: Hs.113290
Expression variation in blood from EXPOLDB for MAGEB3
UniProtKB/Swiss-Prot: MAGB3_HUMAN, O15480
Tissue specificity: Expressed in testis

(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD and/or ⁵MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section

Orthologs for MAGEB3 gene from 4 species

Organism	Gene	Locus	Description	Human Similarity	NCBI accessions
chimpanzee (Pan troglodytes)	MAGEB3¹	--	melanoma antigen family B, 3	99.13(n) 97.4(a)	473547 XM_528919.2 XP_528919.1
cow (Bos taurus)	MAGEB3¹	--	melanoma antigen family B, 3	74.77(n) 62.93(a)	509870 XM_586930.1 XP_586930.1
rat (Rattus norvegicus)	Mageb3¹	--	melanoma antigen family B, 3	73.54(n) 62.15(a)	302746 XM_229038.3 XP_229038.3
mouse (Mus musculus)	Gm5758⁵ EG436212¹	X⁵	predicted gene 5758⁵ predicted gene, EG436212¹	73.58(n)¹ 62.85(a)¹	436212¹ XM_488356.1¹ XP_488356.1¹

About this table Species with no ortholog for MAGEB3

ENSEMBL Gene Tree for MAGEB3

(Paralogs according to ¹HomoloGene
and ²Ensembl, Pseudogenes according to ³Pseudogene.org)
About This Section

Paralogs for MAGEB3 gene

MAGEB5² MAGEB16² MAGEB10² MAGEB18²

Pseudogenes

2 related pseudogenes

(According to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
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10/47 NCBI SNPs in MAGEB3 are shown (see all 47 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay) (see all 45)

AB	SNP ID	Valid	Chr X pos	Sequence	Recs	AA Chg	Type	More	Recs	Allele freq	Pop	Total sample	More
AB	Genomic Data				Transcription Data				Allele Frequencies
				--	--	--		--	--				--
	rs2529554^1,2	C,F,H	30165441(+)	`CTGTGT/CTTTAA`	1	--	ut3¹		11		EU EA WA NA	764
	rs2071309^1,2	C,F,H	30164297(+)	`TCTAAC/TCATGA`	1	I/T	mis¹		11		EU EA WA NA	770
	rs2071308^1,2	C,F,H	30164282(+)	`GTCTCG/ACACAG`	1	H/R	mis¹ ese³		4		EU EA WA	410
	rs7063368^1,2	H	30165236(+)	`TAAGAG/ATAAAA`	1	--	ut3¹		4		EU EA WA	418
	rs2529547^1,2	H	30157062(+)	`GTTGTG/TGGAAA`	1	--	ng5¹		4		EU EA WA	416
	rs2856732^1,2	H	30158196(+)	`TAAAAC/GAGAAG`	1	--	ng5¹ tfbs³		4		EU EA WA	418
--	rs2529548^1,2	C	30157599(+)	`aaaaaA/Taaata`	1	--	ng5¹ tfbs³ trp³		0	--	--	--	--
--	rs5973797^1,2	A	30157466(+)	`ggacaC/Tctgta`	1	--	ng5¹		0	--	--	--	--
	rs2071310^1,2	A,C,F,H	30164451(+)	`GTGGTA/GTTTGG`	1	V/V	syn¹		9		EU EA WA NA	709
	rs2529552^1,2	A,C,F,H,O	30161889(+)	`TCCTGG/TTTGAG`	1	--	int¹		8		EU EA WA NA	609

About this table

HapMap Linkage Disequilibrium images for MAGEB3 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 300152

(Possibly Related Articles in Doctor's Guide)
About This Section

(in PubMed. Associations of this gene to articles via ¹Novoseek, ²HGNC, ³Entrez Gene, ⁴UniProtKB/Swiss-Prot, ⁵UniProtKB/TrEMBL, ⁶GAD, and/or ⁷PharmGKB)
About This Section

6 PubMed articles for MAGEB3 gene:

Two members of the human MAGEB gene family located in Xp21.3 are expressed in tumors of various histological origins. (PubMed id 9441743)^{2, 3, 4} Lurquin C....Boon T. (1997)
The DNA sequence of the human X chromosome. (PubMed id 15772651)^{3, 4} Ross M.T.... Bentley D.R. (2005)
The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)^{3, 4} Gerhard D.S....Malek J. (2004)
Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)^{3, 4} Ota T....Sugano S. (2004)
Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)³ Strausberg R.L....Marra M.A. (2002)
MAGE-B5, MAGE-B6, MAGE-C2, and MAGE-C3: four new members of the MAGE family with tumor-specific expression. (PubMed id 10861452)³ Lucas S.... Boon T. (2000)

(in PubMed, OMIM, and NCBI Bookshelf)
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		AND	OR
Aliases
Free Text

Query String		PubMed OMIM NCBI Bookshelf
	(Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
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Entrez Gene: 4114	HGNC: 6810	AceView: MAGEB2andMAGEB3	Ensembl:ENSG00000198798	euGenes: HUgn4114
ECgene: MAGEB3	H-InvDB: MAGEB3

(According to HUGE)
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(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
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Name	Description
ATLAS Chromosomes in Cancer entry for MAGEB3	Genetics and Cytogenetics in Oncology and Haematology

Licensable Technologies for MAGEB3
(Available from WIS Yeda, Salk, Tufts)

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(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
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Products for MAGEB3:

TaqMan ® Gene Expression Assays

TaqMan ® Genotyping Assays

Free SNP selection tool

Invitrogen iPath Pathways	Invitrogen BLOCK-iT™ RNAi
Invitrogen Antibodies	Invitrogen Assays
Invitrogen Clones	Invitrogen Q-PCR Products
Invitrogen Human Recombinant Kinases	Invitrogen Custom Antibody and Peptide Service
Invitrogen Proteins / Assays / Screening Services	Search Invitrogen catalog for MAGEB3-related products

	Millipore Custom Antibody & Bulk Services
	Millipore Preclinical / Clinical Development Services
	Millipore Immunoassay Services
	Millipore Target Screening & Profiling Services

	Predesigned and custom siRNAs for MAGEB3		Browse antibodies at Sigma-Aldrich
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MAGEB3 Gene

protein-coding GIFtS: 50

Aliases &
Descriptions
for MAGEB3

Summaries
for MAGEB3

Genomic Location
for MAGEB3

Proteins
for MAGEB3

Protein Domains/
Families
for MAGEB3

Gene Function
for MAGEB3

Pathways & Interactions
for MAGEB3

Drugs & Compounds
for MAGEB3

Transcripts
for MAGEB3

Expression
for MAGEB3

Orthologs
for MAGEB3

Paralogs
for MAGEB3

SNPs/Variants
for MAGEB3

Disorders & Mutations
for MAGEB3

Medical News
for MAGEB3

Publications
for MAGEB3

Search for MAGEB3

Genome Databases showing MAGEB3

Other Databases showing MAGEB3

Specialized Databases showing MAGEB3

Licensable Technologies
for MAGEB3

Services
for MAGEB3

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

MAGEB3 Gene

protein-coding GIFtS: 50

Aliases & Descriptionsfor MAGEB3

Summariesfor MAGEB3

Genomic Locationfor MAGEB3

Proteinsfor MAGEB3

Protein Domains/Familiesfor MAGEB3

Gene Functionfor MAGEB3

Pathways & Interactionsfor MAGEB3

Drugs & Compoundsfor MAGEB3

Transcriptsfor MAGEB3

Expression for MAGEB3

Orthologsfor MAGEB3

Paralogsfor MAGEB3

SNPs/Variantsfor MAGEB3

Disorders & Mutationsfor MAGEB3

Medical Newsfor MAGEB3

Publicationsfor MAGEB3

Search for MAGEB3

Genome Databases showing MAGEB3

Other Databases showing MAGEB3

Specialized Databases showing MAGEB3

Licensable Technologiesfor MAGEB3

Servicesfor MAGEB3

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

Aliases &
Descriptions
for MAGEB3

Summaries
for MAGEB3

Genomic Location
for MAGEB3

Proteins
for MAGEB3

Protein Domains/
Families
for MAGEB3

Gene Function
for MAGEB3

Pathways & Interactions
for MAGEB3

Drugs & Compounds
for MAGEB3

Transcripts
for MAGEB3

Expression
for MAGEB3

Orthologs
for MAGEB3

Paralogs
for MAGEB3

SNPs/Variants
for MAGEB3

Disorders & Mutations
for MAGEB3

Medical News
for MAGEB3

Publications
for MAGEB3

Licensable Technologies
for MAGEB3

Services
for MAGEB3