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MAGEB2 Gene

protein-coding GIFtS: 59

GC0XP030143

melanoma antigen family B, 2
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc , and/or ⁷Ensembl, ⁸miRBase)
About This Section

Aliases
CT3.2 ^¹, ², ³
DAM6 ^¹, ², ³, ⁵
MAGE-XP-2 ^¹, ²
MGC26438 ^¹, ²
OTTHUMP00000023098 ^²

Descriptions
Cancer/testis antigen 3.2 ^³
DSS-AHC critical interval MAGE superfamily 6 ^³
DSS/AHC critical interval MAGE superfamily 6 ^²
MAGE XP-2 antigen ^³
MAGE-B2 antigen ^³
cancer/testis antigen family 3, member 2 ^²
melanoma antigen family B, 2 ^²
melanoma-associated antigen B2 ^²

External Ids
HGNC: 6809¹
Entrez Gene: 4113²
UniProtKB: O15479³
Ensembl: ENSG00000099399⁷

Search outside databases for aliases for MAGEB2 gene

Previous GC identifers: GC0XP028733 GC0XP028917 GC0XP029435 GC0XP029595 GC0XP029993

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for MAGEB2:

This gene is a member of the MAGEB gene family. The members of this family have their entire coding

sequences located in the last exon, and the encoded proteins show 50 to 68% sequence identity to

each other. The promoters and first exons of the MAGEB genes show considerable variability,

suggesting that the existence of this gene family enables the same function to be expressed under

different transcriptional controls. This gene is localized in the DSS (dosage-sensitive sex

reversal) critical region. It is expressed in testis and placenta, and in a significant fraction

of tumors of various histological types. The MAGEB genes are clustered on chromosome Xp22-p21.

[provided by RefSeq]

Gene Wiki entry for MAGEB2

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:

UCSC Golden Path with GeneCards custom track

Transcription factor binding sites upstream to the MAGEB2 gene

Entrez Gene cytogenetic band: Xp21.3 Ensembl cytogenetic band: Xp21.2 HGNC cytogenetic band: Xp21.3

MAGEB2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome X GeneLoc Exon Structure

GeneLoc location for GC0XP030143: (about GC identifiers)

Start:	30,143,601 bp from pter
End:	30,148,127 bp from pter
Size:	4,527 bases
Orientation:	plus strand

RefSeq DNA sequence:

NC_000023.9 NT_011757.15

(According to ¹UniProtKB, and/or Ensembl, Phosphorylation sites according to ²Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: MAGB2_HUMAN, O15479 (See protein sequence)

Recommended Name: Melanoma-associated antigen B2

Size: 319 amino acids; 35276 Da

Secondary accessions: O75860

Post-translational modifications:

View phosphorylation sites using PhosphoSite²

REFSEQ proteins: NP_002355.2

ENSEMBL proteins:

ENSP00000368273

Human Recombinant Proteins

	Browse Drug Discovery Central at Invitrogen for human recombinant proteins
	Browse Purified and Recombinant Proteins at Millipore
	Browse Human Recombinant Proteins at Sigma-Aldrich
	Browse R&D Systems for human recombinant proteins
	Browse recombinant and purified proteins available from Enzo Life Sciences
	Recombinant Proteins from Abcam (MAGEB2)
	Human Recombinant Proteins from Abnova (MAGEB2)

Browse Origene for full length recombinant human proteins expressed in human HEK293 cells

Antibodies for MAGEB2:

	Browse Antibodies Central at Invitrogen
	Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
	Browse Antibodies at Sigma-Aldrich
	Browse R&D Systems for Antibodies
	Antibodies from Abcam (MAGEB2), each with their Abpromise^SM
	Monoclonal and Polyclonal Antibodies from Abnova (MAGEB2)
	Novus Biologicals Antibody for MAGEB2

Assays for MAGEB2:

	Browse Invitrogen for biochemical assays
	Browse Kits and Assays available from Millipore
	Browse R&D Systems for biochemical assays
	Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

Graphical View of Domain Structure for InterPro Entry O15479

ProtoNet protein and cluster: O15479

1 Blocks protein family: IPB002190 MAGE protein

UniProtKB/Swiss-Prot: MAGB2_HUMAN, O15479

Similarity: Contains 1 MAGE domain

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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Inhib. RNA:		Invitrogen RNAi Products for gene knock-down (MAGEB2)
		Browse for Gene Knock-down Tools from Millipore
		Browse Abnova for Chimera RNAi Products

OriGene 29mer shRNA kit in GFP-retroviral vector: NM_002364

Applied Biosystems Silencer® siRNAs for MAGEB2

Sigma-Aldrich siRNA for MAGEB2

Sigma-Aldrich shRNA for MAGEB2

Explore Sigma-Aldrich super-pooled esiRNAs

Clones:		Invitrogen Clones for MAGEB2
		Browse Clones for the Expression of Recombinant Proteins Available from Millipore

OriGene GFP tagged cDNA clone in CMV expression vector: NM_002364
Myc/DDK tagged cDNA clone in CMV expression vector: NM_002364
untagged cDNA clone in CMV expression vector: NM_002364

Primers:

Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers

OriGene genome-wide validated SYBR primer pairs: NM_002364

Genatlas biochemistry entry for MAGEB2:

melanoma antigen,family B,member 2,expressed in placenta,testis,melanoma and lung carcinomas

1 Gene Ontology (GO) molecular function term (links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005515	protein binding	IPI	17353931

About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to ¹UniProtKB, ²MINT, and/or ³STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

5/19 Interacting proteins for MAGEB2 (O15479¹ ENSP00000368273³) via UniProtKB, MINT, and/or STRING (see all 19 )

Interactant		Interaction Details
GeneCard	External ID(s)	Interaction Details
CSNK2B	P67870¹	EBI-1057615, EBI-348169
LXN	Q9BS40¹	EBI-1057615, EBI-1044504
RPL23A	P62750¹	EBI-1057615, EBI-353254
RPL24	P83731¹	EBI-1057615, EBI-353146
RPL26	P61254¹	EBI-1057615, EBI-352405

About this table

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences

Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for MAGEB2

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib. RNA:		Invitrogen RNAi Products for gene knock-down (MAGEB2)
		Browse for Gene Knock-down Tools from Millipore
		Browse Abnova for Chimera RNAi Products

OriGene 29mer shRNA kit in GFP-retroviral vector: NM_002364

Sigma-Aldrich siRNA for MAGEB2

Sigma-Aldrich shRNA for MAGEB2

Explore Sigma-Aldrich super-pooled esiRNAs

Applied Biosystems Silencer® siRNAs:

NM_002364

REFSEQ mRNAs for MAGEB2 gene:

NM_002364.4

Applied Biosystems TaqMan ® Gene Expression Assays:

NM_002364

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_002364
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_002364
                                 untagged cDNA clone in CMV expression vector: NM_002364

Additional cDNA sequence:

AF015766.1 AK223081.1 AK313347.1 BC026071.2 CR590008.1 CR593708.1 CR611588.1 CR618278.1

CR618499.1 CR621494.1

2 DOTS entries:

DT.307058 DT.95163778

24/61 AceView cDNA sequences (see all 61 ):

CR593708 BX107939 CR611588 CR618278 BI520298 NM_002365 CR590008 CR621494

CR618499 BU175305 AA936574 AL525917 AK057441 AA889133 CA428956 BU629619

CA423793 AI637744 BC074756 AI479347 BU188463 AW589912 BM471242 BC026071

highest scoring ESTs for MAGEB2:

AA815379 AA889133 AF015766 AI637744 AL530590 AL562631 AL580893 AU099704 AW589912 AW614003

Unigene Cluster for MAGEB2:

Melanoma antigen family B, 2

Hs.113824 [show with all ESTs]

Unigene Representative Sequence: NM_002364

GeneLoc Exon Structure

2 Alternative Splicing Database (ASD) splice patterns (SP) for MAGEB2

ExUns:	1	^	2a	·	2b	·	2c
SP1:			-
SP2:

About this scheme

ECgene alternative splicing isoforms for MAGEB2

1 Ensembl transcript including schematic representation:

ENST00000378988

(Experimental results according to ¹GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to ²GeneAnnot , ³GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

MAGEB2 expression in normal and diseased human tissues

Applied Biosystems TaqMan ® Gene Expression Assays for MAGEB2

¹ / ² / ³

3 probe-sets matching MAGEB2 gene

Affymetrix probe-set	Array	# genes	Sensitivity	Specificity	Correlation	Length	Gb_Accession	Consensus	Uniqueness	Score	Rank
Affymetrix probe-set	Array	GeneAnnot data			GeneNote data		GeneTide data
35097_at²^{, 3}	U95-A	1	1.00	1.00	1.00	1.00	U93163	0.20	0.33	0.27	1
206218_at²^{, 3}	U133-A	1	1.00	1.00	--	--	NM_002364	0.60	1.00	0.82	1
206218_at²	U133Plus2	1	1.00	1.00	--	--	--	--	--	--	--

About this table

Data from

(Publications) and GNF BioGPS
About these images
About these images

CGAP SAGE TAG: AATAACTTGT

SOURCE GeneReport for Unigene cluster: Hs.113824
Expression variation in blood from EXPOLDB for MAGEB2
UniProtKB/Swiss-Prot: MAGB2_HUMAN, O15479
Tissue specificity: Expressed in testis and placenta, and in a significant fraction of tumors of
various histologic types

(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD and/or ⁵MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section

Orthologs for MAGEB2 gene from 3 species

Organism	Gene	Locus	Description	Human Similarity	NCBI accessions
chimpanzee (Pan troglodytes)	MAGEB2¹	--	melanoma antigen family B, 2	98.75(n) 96.55(a)	465553 XM_520990.2 XP_520990.2
rat (Rattus norvegicus)	Mageb4¹	--	melanoma antigen family B, 4	67.63(n) 52.58(a)	503441 XM_578989.1 XP_578989.1
mouse (Mus musculus)	Gm41¹	--	gene model 41, (NCBI)	67.2(n) 52.09(a)	245502 XM_111935.6 XP_111935.5

About this table Species with no ortholog for MAGEB2

ENSEMBL Gene Tree for MAGEB2

(Paralogs according to ¹HomoloGene
and ²Ensembl, Pseudogenes according to ³Pseudogene.org)
About This Section

Paralogs for MAGEB2 gene

MAGEB17² MAGEB6² MAGEB1² MAGEB4²

Pseudogenes

1 related pseudogene

(According to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section

10/42 NCBI SNPs in MAGEB2 are shown (see all 42 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay) (see all 45)

AB	SNP ID	Valid	Chr X pos	Sequence	Recs	AA Chg	Type	More	Recs	Pop	Total sample	More
AB	Genomic Data				Transcription Data				Allele Frequencies
				--	--	--		--	--			--
	rs2238920^1,2	A,C,F,H,O	30142097(+)	`CCTCAG/AGTCAG`	1	--	ng5¹ tfbs³		7	EU EA WA NA	532
	rs1053918^1,2	A,C,F,H,O	30148011(+)	`AGAGGT/GGTAAA`	1	--	ut3¹ ese³		7	EU EA WA NA	560
	rs5972091^1,2	A,C,F,H	30147947(+)	`TTAGAG/ATAGAA`	1	--	ut3¹		12	EU EA WA NS NA	666
	rs11797594^1,2	A,C,F,H	30142113(+)	`GATGGA/CGTGCC`	1	--	ng5¹		7	EU EA WA NA	526
	rs11798106^1,2	A,C,F,H	30142985(+)	`AAACCC/GACGCC`	1	--	ng5¹		9	EU EA WA NA	744
	rs12009082^1,2	A,C,F,H	30143343(+)	`ACGCTC/TAGATT`	1	--	ng5¹		2	EU WA	208
	rs11095177^1,2	A,C,F,H	30142596(+)	`atgttA/Caaatt`	1	--	ng5¹ tfbs³		4	EU EA WA	390
	rs2529541^1,2	A,C,F,H	30146799(+)	`CCCAGA/GAGCCT`	1	K/E	mis¹ ese³		4	EU EA WA	404
	rs5972090^1,2	C,F,H	30147570(+)	`AAGCCG/AGAGTC`	1	R/G	mis¹ ese³		8	EU EA WA	721
	rs2238922^1,2	C,F,H	30142394(+)	`ggtaaA/Ggggga`	1	--	ng5¹		4	EU EA WA	392

About this table

HapMap Linkage Disequilibrium images for MAGEB2 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, Novoseek, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 300098

1 Novoseek disease relationship for MAGEB2 gene

Disease	Score	Articles	PubMed IDs for Articles with Shared Sentences (# sentences)
tumors	19.47	2	9441743 (2)

About this table

(Possibly Related Articles in Doctor's Guide)
About This Section

(in PubMed. Associations of this gene to articles via ¹Novoseek, ²HGNC, ³Entrez Gene, ⁴UniProtKB/Swiss-Prot, ⁵UniProtKB/TrEMBL, ⁶GAD, and/or ⁷PharmGKB)
About This Section

10/16 PubMed articles for MAGEB2 gene (see all 16 ):

Two members of the human MAGEB gene family located in Xp21.3 are expressed in tumors of various histological origins. (PubMed id 9441743)^{1, 2, 3, 4} Lurquin C....Boon T. (1997)
MAGE Xp-2: a member of the MAGE gene family isolated from an expression library using systemic lupus erythematosus sera. (PubMed id 9538511)^{1, 3, 4} McCurdy D.K.... Gatti R.A. (1998)
A family of rapidly evolving genes from the sex reversal critical region in Xp21. (PubMed id 8535061)^{1, 3, 4} Dabovic B.... Camerino G. (1995)
The DNA sequence of the human X chromosome. (PubMed id 15772651)^{3, 4} Ross M.T.... Bentley D.R. (2005)
The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)^{3, 4} Gerhard D.S....Malek J. (2004)
A quantitative atlas of mitotic phosphorylation. (PubMed id 18669648)⁴ Dephoure N.... Gygi S.P. (2008)
Valproate induces widespread epigenetic reprogramming which involves demethylation of specific genes. (PubMed id 17012225)³ Milutinovic S....Szyf M. (2007)
Large-scale mapping of human protein-protein interactions by mass spectrometry. (PubMed id 17353931)³ Ewing R.M....Figeys D. (2007)
Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)³ Strausberg R.L....Marra M.A. (2002)
Hypertonicity induction of melanoma antigen, a tumor-associated antigen. (PubMed id 12018852)³ Park J.H. and Lee S.W. (2002)

(in PubMed, OMIM, and NCBI Bookshelf)
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		AND	OR
Aliases
Free Text

Query String		PubMed OMIM NCBI Bookshelf
	(Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 4113	HGNC: 6809	AceView: MAGEB2andMAGEB3	Ensembl:ENSG00000099399	euGenes: HUgn4113
ECgene: MAGEB2	H-InvDB: MAGEB2

(According to HUGE)
About This Section

(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
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Name	Description
ATLAS Chromosomes in Cancer entry for MAGEB2	Genetics and Cytogenetics in Oncology and Haematology

Licensable Technologies for MAGEB2
(Available from WIS Yeda, Salk, Tufts)

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(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
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Products for MAGEB2:

TaqMan ® Gene Expression Assays

TaqMan ® Genotyping Assays

Free SNP selection tool

Invitrogen iPath Pathways	Invitrogen BLOCK-iT™ RNAi
Invitrogen Antibodies	Invitrogen Assays
Invitrogen Clones	Invitrogen Q-PCR Products
Invitrogen Human Recombinant Kinases	Invitrogen Custom Antibody and Peptide Service
Invitrogen Proteins / Assays / Screening Services	Search Invitrogen catalog for MAGEB2-related products

	Millipore Custom Antibody & Bulk Services
	Millipore Preclinical / Clinical Development Services
	Millipore Immunoassay Services
	Millipore Target Screening & Profiling Services

	Predesigned and custom siRNAs for MAGEB2		Browse antibodies at Sigma-Aldrich
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	Lentivirus-delivered shRNAs for MAGEB2		Browse small molecules at Sigma-Aldrich
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MAGEB2 Gene

protein-coding GIFtS: 59

Aliases &
Descriptions
for MAGEB2

Summaries
for MAGEB2

Genomic Location
for MAGEB2

Proteins
for MAGEB2

Protein Domains/
Families
for MAGEB2

Gene Function
for MAGEB2

Pathways & Interactions
for MAGEB2

Drugs & Compounds
for MAGEB2

Transcripts
for MAGEB2

Expression
for MAGEB2

Orthologs
for MAGEB2

Paralogs
for MAGEB2

SNPs/Variants
for MAGEB2

Disorders & Mutations
for MAGEB2

Medical News
for MAGEB2

Publications
for MAGEB2

Search for MAGEB2

Genome Databases showing MAGEB2

Other Databases showing MAGEB2

Specialized Databases showing MAGEB2

Licensable Technologies
for MAGEB2

Services
for MAGEB2

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

MAGEB2 Gene

protein-coding GIFtS: 59

Aliases & Descriptionsfor MAGEB2

Summariesfor MAGEB2

Genomic Locationfor MAGEB2

Proteinsfor MAGEB2

Protein Domains/Familiesfor MAGEB2

Gene Functionfor MAGEB2

Pathways & Interactionsfor MAGEB2

Drugs & Compoundsfor MAGEB2

Transcriptsfor MAGEB2

Expression for MAGEB2

Orthologsfor MAGEB2

Paralogsfor MAGEB2

SNPs/Variantsfor MAGEB2

Disorders & Mutationsfor MAGEB2

Medical Newsfor MAGEB2

Publicationsfor MAGEB2

Search for MAGEB2

Genome Databases showing MAGEB2

Other Databases showing MAGEB2

Specialized Databases showing MAGEB2

Licensable Technologiesfor MAGEB2

Servicesfor MAGEB2

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

Aliases &
Descriptions
for MAGEB2

Summaries
for MAGEB2

Genomic Location
for MAGEB2

Proteins
for MAGEB2

Protein Domains/
Families
for MAGEB2

Gene Function
for MAGEB2

Pathways & Interactions
for MAGEB2

Drugs & Compounds
for MAGEB2

Transcripts
for MAGEB2

Expression
for MAGEB2

Orthologs
for MAGEB2

Paralogs
for MAGEB2

SNPs/Variants
for MAGEB2

Disorders & Mutations
for MAGEB2

Medical News
for MAGEB2

Publications
for MAGEB2

Licensable Technologies
for MAGEB2

Services
for MAGEB2