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MAGEA8 Gene

protein-coding GIFtS: 49

GC0XP148770

melanoma antigen family A, 8
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
(Previous symbol: MAGE8)

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc , and/or ⁷Ensembl, ⁸miRBase)
About This Section

Aliases
CT1.8 ^¹, ², ³
MAGE8 ^², ³, ⁵
MGC2182 ^¹, ²
OTTHUMP00000024218 ^²

Descriptions
Cancer/testis antigen 1.8 ^³
MAGE-8 antigen ^², ³
cancer/testis antigen family 1, member 8 ^²
melanoma antigen family A, 8 ^²

External Ids
HGNC: 6806¹
Entrez Gene: 4107²
UniProtKB: P43361³
Ensembl: ENSG00000156009⁷

Search outside databases for aliases for MAGEA8 gene

Previous GC identifers: GC0XP143633 GC0XP146013 GC0XP146853 GC0XP147668 GC0XP148668

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for MAGEA8:

This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50

to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show

considerable variability, suggesting that the existence of this gene family enables the same

function to be expressed under different transcriptional controls. The MAGEA genes are clustered

at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as

dyskeratosis congenita. [provided by RefSeq]

UniProtKB/Swiss-Prot: MAGA8_HUMAN, P43361

Function: Not known, though may play a role in embryonal development and tumor transformation or

aspects of tumor progression

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:

UCSC Golden Path with GeneCards custom track

Transcription factor binding sites upstream to the MAGEA8 gene

Entrez Gene cytogenetic band: Xq28 Ensembl cytogenetic band: Xq28 HGNC cytogenetic band: Xq28

MAGEA8 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome X GeneLoc Exon Structure

GeneLoc location for GC0XP148770: (about GC identifiers)

Start:	148,770,653 bp from pter
End:	148,775,266 bp from pter
Size:	4,614 bases
Orientation:	plus strand

RefSeq DNA sequence:

NC_000023.9 NT_011681.15

(According to ¹UniProtKB, and/or Ensembl, Phosphorylation sites according to ²Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: MAGA8_HUMAN, P43361 (See protein sequence)

Recommended Name: Melanoma-associated antigen 8

Size: 318 amino acids; 35215 Da

Secondary accessions: Q9BUN9

REFSEQ proteins: NP_005355.2

ENSEMBL proteins:

ENSP00000286482

Human Recombinant Proteins

	Browse Drug Discovery Central at Invitrogen for human recombinant proteins
	Browse Purified and Recombinant Proteins at Millipore
	Browse Human Recombinant Proteins at Sigma-Aldrich
	Browse R&D Systems for human recombinant proteins
	Browse recombinant and purified proteins available from Enzo Life Sciences
	Recombinant Proteins from Abcam (MAGEA8)
	Human Recombinant Proteins from Abnova (MAGEA8)

OriGene Purified Recombinant Human Protein: MAGEA8

1 Gene Ontology (GO) cellular component term (links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005575	cellular_component	ND	--

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Antibodies for MAGEA8:

	Browse Antibodies Central at Invitrogen
	Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
	Sigma-Aldrich Antibodies for MAGEA8
	Browse R&D Systems for Antibodies
	Antibodies from Abcam (MAGEA8), each with their Abpromise^SM
	Monoclonal and Polyclonal Antibodies from Abnova (MAGEA8)
	Novus Biologicals Antibodies for MAGEA8

Assays for MAGEA8:

	Browse Invitrogen for biochemical assays
	Browse Kits and Assays available from Millipore
	Browse R&D Systems for biochemical assays
	Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

ProtoNet protein and cluster: P43361

UniProtKB/Swiss-Prot: MAGA8_HUMAN, P43361

Similarity: Contains 1 MAGE domain

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

Inhib. RNA:		Invitrogen RNAi Products for gene knock-down (MAGEA8)
		Browse for Gene Knock-down Tools from Millipore
		Abnova Chimera RNAi Products for Gene knock-down (MAGEA8)

OriGene 29mer shRNA kit in GFP-retroviral vector: NM_005364

Applied Biosystems Silencer® siRNAs for MAGEA8

Sigma-Aldrich siRNA for MAGEA8

Sigma-Aldrich shRNA for MAGEA8

Explore Sigma-Aldrich super-pooled esiRNAs

Clones:		Invitrogen Clones for MAGEA8
		Browse Clones for the Expression of Recombinant Proteins Available from Millipore

OriGene GFP tagged cDNA clone in CMV expression vector: NM_005364
Myc/DDK tagged cDNA clone in CMV expression vector: NM_005364
untagged cDNA clone in CMV expression vector: NM_005364

Primers:

Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers

OriGene genome-wide validated SYBR primer pairs: NM_005364

UniProtKB/Swiss-Prot: MAGA8_HUMAN, P43361

Function: Not known, though may play a role in embryonal development and tumor transformation or

aspects of tumor progression

Genatlas biochemistry entry for MAGEA8:

human melanoma antigen,family A,member 8,expressed in melanoma,testis

1 Gene Ontology (GO) molecular function term (links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0003674	molecular_function	ND	--

About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to ¹UniProtKB, ²MINT, and/or ³STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

1 Gene Ontology (GO) biological process term (links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0008150	biological_process	ND	--

About this table

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences

Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for MAGEA8

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib. RNA:		Invitrogen RNAi Products for gene knock-down (MAGEA8)
		Browse for Gene Knock-down Tools from Millipore
		Abnova Chimera RNAi Products for Gene knock-down (MAGEA8)

OriGene 29mer shRNA kit in GFP-retroviral vector: NM_005364

Sigma-Aldrich siRNA for MAGEA8

Sigma-Aldrich shRNA for MAGEA8

Explore Sigma-Aldrich super-pooled esiRNAs

Applied Biosystems Silencer® siRNAs:

NM_005364

REFSEQ mRNAs for MAGEA8 gene:

NM_005364.3

Applied Biosystems TaqMan ® Gene Expression Assays:

NM_005364

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_005364
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_005364
                                 untagged cDNA clone in CMV expression vector: NM_005364

Additional cDNA sequence:

AK313943.1 BC002455.2 BC012744.2 BC098120.1 BT007340.1 CR619399.1

2 DOTS entries:

DT.207126 DT.100768791

24/33 AceView cDNA sequences (see all 33 ):

NM_005364 BI092675 BX360398 CR619399 BC002455 BQ434005 AA045817 BC012744

BX102591 AI032153 BI087592 BE275893 BX399615 BE729897 BE388427 BG481340

BE407511 BT007340 BI087522 BU188475 BE727811 BX417414 CF994013 BE892022

highest scoring ESTs for MAGEA8:

AA045817 AA045931 AI032153 AL575658 BC002455 BC012744 BE275893 BE387798 BE388427 BE407511

Unigene Cluster for MAGEA8:

Melanoma antigen family A, 8

Hs.522803 [show with all ESTs]

Unigene Representative Sequence: BC002455

GeneLoc Exon Structure

3 Alternative Splicing Database (ASD) splice patterns (SP) for MAGEA8

ExUns:	1a	·	1b	^	2	^	3a	·	3b	^	4a	·	4b	·	4c
SP1:							-
SP2:					-		-
SP3:					-

About this scheme

ECgene alternative splicing isoforms for MAGEA8

1 Ensembl transcript including schematic representation:

ENST00000286482

(Experimental results according to ¹GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to ²GeneAnnot , ³GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

MAGEA8 expression in normal and diseased human tissues

Applied Biosystems TaqMan ® Gene Expression Assays for MAGEA8

¹ / ² / ³

3 probe-sets matching MAGEA8 gene

Affymetrix probe-set	Array	# genes	Sensitivity	Specificity	Correlation	Length	Gb_Accession	Consensus	Uniqueness	Score	Rank
Affymetrix probe-set	Array	GeneAnnot data			GeneNote data		GeneTide data
34576_at²^{, 3}	U95-A	1	1.00	1.00	1.00	1.00	U10693	0.20	1.00	0.72	1
210274_at²^{, 3}	U133-A	1	1.00	1.00	--	--	BC002455	0.80	1.00	0.91	1
210274_at²	U133Plus2	1	1.00	1.00	--	--	--	--	--	--	--

About this table

Data from

(Publications) and GNF BioGPS
About these images
About these images

CGAP SAGE TAG: AGATAAAGAC

SOURCE GeneReport for Unigene cluster: Hs.522803
Expression variation in blood from EXPOLDB for MAGEA8
UniProtKB/Swiss-Prot: MAGA8_HUMAN, P43361
Tissue specificity: Expressed in many tumors of several types, such as melanoma, head and neck
squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for
testis and placenta

(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD and/or ⁵MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section

Orthologs for MAGEA8 gene from 1 species

Organism	Gene	Locus	Description	Human Similarity	NCBI accessions
chimpanzee (Pan troglodytes)	MAGEA8¹	--	melanoma antigen family A, 8	99.58(n) 99.06(a)	473819 XM_529192.2 XP_529192.1

About this table Species with no ortholog for MAGEA8

ENSEMBL Gene Tree for MAGEA8

(Paralogs according to ¹HomoloGene
and ²Ensembl, Pseudogenes according to ³Pseudogene.org)
About This Section

Paralogs for MAGEA8 gene

MAGEA3² MAGEA2B² MAGEA12² MAGEA9² MAGEA9B² MAGEA4² MAGEA11² MAGEA1²

MAGEA6² MAGEA10² MAGEA2²

(According to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section

10/32 NCBI SNPs in MAGEA8 are shown (see all 32 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay) (see all 18)

AB	SNP ID	Valid	Chr X pos	Sequence	Recs	AA Chg	Type	More	Recs	Allele freq	Pop	Total sample	More
AB	Genomic Data				Transcription Data				Allele Frequencies
				--	--	--		--	--				--
--	rs12010332^1,2	C,F,H	148774621(+)	`CCCATC/TCCTGC`	1	S/F	mis¹ ese³		4		EU EA NA	366
	rs5983913^1,2	A,F,H	148769390(+)	`AAACAT/GAGAAA`	1	--	ng5¹		3		EU EA	298
	rs35744768^1,2	C,F	148774066(+)	`AGTTCG/ATTTCC`	1	H/R	mis¹		5		NA EU EA WA	393
	rs7064596^1,2	F,H	148775341(+)	`GGAGAT/CGCTAC`	1	--	ng3¹		4		EU EA WA	406
	rs6641385^1,2	H	148769142(+)	`gctgaC/Gagatt`	1	--	ng5¹		4		EU EA WA	400
	rs5983916^1,2	A,C,F,H	148774385(+)	`GAAGCA/GTTGAG`	1	A/A	syn¹		8		EU EA NA	572
	rs5983915^1,2	A,C,F,H	148774301(+)	`AGTACG/ACCCAA`	1	T/T	syn¹ ese³		12		EU EA WA NA	836
	rs5983914^1,2	A,C,F,H	148771995(+)	`GATGAA/CTGGGG`	1	--	int¹		4		EU EA WA	418
--	rs45519637^1,2	--	148774431(+)	`TCTATG/TGGAAG`	1	W/G	mis¹		0	--	--	--	--
--	rs45564732^1,2	--	148774475(+)	`GAGAAC/GTACCT`	1	N/K	mis¹		0	--	--	--	--

About this table

HapMap Linkage Disequilibrium images for MAGEA8 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 300341

(Possibly Related Articles in Doctor's Guide)
About This Section

(in PubMed. Associations of this gene to articles via ¹Novoseek, ²HGNC, ³Entrez Gene, ⁴UniProtKB/Swiss-Prot, ⁵UniProtKB/TrEMBL, ⁶GAD, and/or ⁷PharmGKB)
About This Section

8 PubMed articles for MAGEA8 gene:

The melanoma antigen gene (MAGE) family is clustered in the chromosomal band Xq28. (PubMed id 8575766)^{2, 3} Rogner U.C....Poustka A. (1995)
Structure, chromosomal localization, and expression of 12 genes of the MAGE family. (PubMed id 7927540)^{3, 4} De Plaen E.... Boon T. (1994)
The DNA sequence of the human X chromosome. (PubMed id 15772651)⁴ Ross M.T.... Bentley D.R. (2005)
The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)⁴ Gerhard D.S....Malek J. (2004)
Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)³ Strausberg R.L....Marra M.A. (2002)
Hepatitis C virus core protein interacts with 14-3-3 protein and activates the kinase Raf-1. (PubMed id )⁴ Aoki H.... Hino O. (2000)
Identification, expression, and nuclear location of murine Mage-b2 protein, a tumor-associated antigen. (PubMed id 11211869)³ Lee J.H....Park J.H. (2000)
Quantitative evaluation of the expression of MAGE genes in tumors by limiting dilution of cDNA libraries. (PubMed id 10521804)³ Serrano A....Boon T. (1999)

(in PubMed, OMIM, and NCBI Bookshelf)
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		AND	OR
Aliases
Free Text

Query String		PubMed OMIM NCBI Bookshelf
	(Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 4107	HGNC: 6806	AceView: MAGEA8	Ensembl:ENSG00000156009	euGenes: HUgn4107
ECgene: MAGEA8	H-InvDB: MAGEA8

(According to HUGE)
About This Section

(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

Name	Description
ATLAS Chromosomes in Cancer entry for MAGEA8	Genetics and Cytogenetics in Oncology and Haematology

Licensable Technologies for MAGEA8
(Available from WIS Yeda, Salk, Tufts)

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(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
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Products for MAGEA8:

TaqMan ® Gene Expression Assays

TaqMan ® Genotyping Assays

Free SNP selection tool

Invitrogen iPath Pathways	Invitrogen BLOCK-iT™ RNAi
Invitrogen Antibodies	Invitrogen Assays
Invitrogen Clones	Invitrogen Q-PCR Products
Invitrogen Human Recombinant Kinases	Invitrogen Custom Antibody and Peptide Service
Invitrogen Proteins / Assays / Screening Services	Search Invitrogen catalog for MAGEA8-related products

	Millipore Custom Antibody & Bulk Services
	Millipore Preclinical / Clinical Development Services
	Millipore Immunoassay Services
	Millipore Target Screening & Profiling Services

	Predesigned and custom siRNAs for MAGEA8		Antibodies for MAGEA8
	Explore super-pooled esiRNAs		Browse proteins at Sigma-Aldrich
	Lentivirus-delivered shRNAs for MAGEA8		Browse small molecules at Sigma-Aldrich
	"Your Favorite Gene" Pathways		feedback

	Antibodies		Primer Pairs
	Cell Culture Products		ELISAs
	Flow Cytometry Kits		Protease Activity Assays & Reagents
	Cell Selection/Detection Kits & Reagents		ELISA/Assay Development Kits & Reagents
	Multiplex/Array Assay Kits & Reagents		ELISpot Kits & Development Modules
	Recombinant & Natural Proteins

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	Custom Service for Rabbit Pab from Full-length Protein

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	Antibodies (MAGEA8)

	Tagged/untagged cDNA clones
	Validated SYBR primer pairs
	Purified proteins from HEK293T cells
	Western blot validated antibodies
	shRNA in GFP-retroviral vector

Search Tocris compounds for MAGEA8

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Antibodies for MAGEA8

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MAGEA8 Gene

protein-coding GIFtS: 49

Aliases &
Descriptions
for MAGEA8

Summaries
for MAGEA8

Genomic Location
for MAGEA8

Proteins
for MAGEA8

Protein Domains/
Families
for MAGEA8

Gene Function
for MAGEA8

Pathways & Interactions
for MAGEA8

Drugs & Compounds
for MAGEA8

Transcripts
for MAGEA8

Expression
for MAGEA8

Orthologs
for MAGEA8

Paralogs
for MAGEA8

SNPs/Variants
for MAGEA8

Disorders & Mutations
for MAGEA8

Medical News
for MAGEA8

Publications
for MAGEA8

Search for MAGEA8

Genome Databases showing MAGEA8

Other Databases showing MAGEA8

Specialized Databases showing MAGEA8

Licensable Technologies
for MAGEA8

Services
for MAGEA8

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

MAGEA8 Gene

protein-coding GIFtS: 49

Aliases & Descriptionsfor MAGEA8

Summariesfor MAGEA8

Genomic Locationfor MAGEA8

Proteinsfor MAGEA8

Protein Domains/Familiesfor MAGEA8

Gene Functionfor MAGEA8

Pathways & Interactionsfor MAGEA8

Drugs & Compoundsfor MAGEA8

Transcriptsfor MAGEA8

Expression for MAGEA8

Orthologsfor MAGEA8

Paralogsfor MAGEA8

SNPs/Variantsfor MAGEA8

Disorders & Mutationsfor MAGEA8

Medical Newsfor MAGEA8

Publicationsfor MAGEA8

Search for MAGEA8

Genome Databases showing MAGEA8

Other Databases showing MAGEA8

Specialized Databases showing MAGEA8

Licensable Technologiesfor MAGEA8

Servicesfor MAGEA8

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

Aliases &
Descriptions
for MAGEA8

Summaries
for MAGEA8

Genomic Location
for MAGEA8

Proteins
for MAGEA8

Protein Domains/
Families
for MAGEA8

Gene Function
for MAGEA8

Pathways & Interactions
for MAGEA8

Drugs & Compounds
for MAGEA8

Transcripts
for MAGEA8

Expression
for MAGEA8

Orthologs
for MAGEA8

Paralogs
for MAGEA8

SNPs/Variants
for MAGEA8

Disorders & Mutations
for MAGEA8

Medical News
for MAGEA8

Publications
for MAGEA8

Licensable Technologies
for MAGEA8

Services
for MAGEA8