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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

MAGEA2B Gene

protein-coding   GIFtS: 48
GCID: GC0XP151883

melanoma antigen family A, 2B

 Explore 7 diseases affiliated with
MAGEA2B via our new
 Human Malady Compendium 
Biological research products
for MAGEA2B
    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section

Aliases
Melanoma Antigen Family A, 2B1 2     MAGEA2b1
MAGE21 2 3     MGC169731
MAGEA21 2 3     Melanoma Antigen 22
Cancer/Testis Antigen 1.22 3     Melanoma Antigen, Family A, 2, Copy B2
CT1.22 3     Melanoma-Associated Antigen 22
MAGE-2 Antigen2 3     MAGEA2A3

External Ids:    HGNC: 193401   Entrez Gene: 2667402   Ensembl: ENSG000001833057   OMIM: 3005495   UniProtKB: P433563   

Export aliases for MAGEA2B gene to outside databases

Previous GC identifers: GC0XP150502 GC0XP151538 GC0XM151670 GC0XP151633


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for MAGEA2B:
This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence
identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting
that the existence of this gene family enables the same function to be expressed under different transcriptional
controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary
disorders, such as dyskeratosis congenita. This gene has two identical copies at different loci. (provided by RefSeq,
Jul 2008)

UniProtKB/Swiss-Prot: MAGA2_HUMAN, P43356
Function: Reduces p53/TP53 transactivation function through recruitment of HDAC3 to p53/TP53 transcription sites. Also
represses p73/TP73 activity. Proposed to enhance ubiquitin ligase activity of RING-type zinc finger-containing E3
ubiquitin-protein ligases. In vitro enhances ubiquitin ligase activity of TRIM28 and stimulates p53/TP53
ubiquitination by TRIM28 potentially in presence of Ubl-conjugating enzyme UBE2H. Proposed to act through recruitment
and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. May play a role in embryonal
development and tumor transformation or aspects of tumor progression. In vitro promotes cell viability in melanoma
cell lines. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes




(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000023.10  NT_167198.1  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the MAGEA2B gene promoter:
         p53   HNF-1   FOXJ2 (long isoform)   Olf-1   FOXJ2   HNF-1A   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidMAGEA2B promoter sequence
   Search SABiosciences Chromatin IP Primers for MAGEA2B

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat MAGEA2B


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: Xq28   Ensembl cytogenetic band:  Xq28   HGNC cytogenetic band: Xq28

MAGEA2B Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
MAGEA2B gene location

GeneLoc information about chromosome X         GeneLoc Exon Structure

GeneLoc location for GC0XP151883:  view genomic region     (about GC identifiers)

Start:
151,883,082 bp from pter      End:
151,887,096 bp from pter
Size:
4,015 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: MAGA2_HUMAN, P43356 (See protein sequence)
Recommended Name: Melanoma-associated antigen 2  
Size: 314 amino acids; 35055 Da
Subunit: Interacts with TRIM28 and UBE2H. Interacts with HDAC3
Subcellular location: Nucleus
Secondary accessions: A8K328 Q32NC6

Explore the universe of human proteins at neXtProt for MAGEA2B: NX_P43356

Post-translational modifications:

  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_P43356

  • MAGEA2B Protein expression data from MOPED and PaxDb:    About this image 
    Estimated protein expression log10 (pmol).

    REFSEQ proteins: NP_705692.1  
    ENSEMBL proteins: 
     ENSP00000359316   ENSP00000395386   ENSP00000389223   ENSP00000389999   ENSP00000397216  
     ENSP00000413384   ENSP00000386463   ENSP00000333487  

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    Uscn Proteins for MAGEA2B

    Gene Ontology (GO): 1 cellular component term (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634nucleus IDA16847267


    MAGEA2B for ontologies           About GeneDecksing



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    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    MAGEA2B for domains           About GeneDecksing

    2 InterPro domains/families:
     IPR021072 Melanoma_ass_antigen_N
     IPR002190 MAGE

    Graphical View of Domain Structure for InterPro Entry P43356

    ProtoNet protein and cluster: P43356

    1 Blocks protein family: IPB002190 MAGE protein

    UniProtKB/Swiss-Prot: MAGA2_HUMAN, P43356
    Similarity: Contains 1 MAGE domain


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section

    Function Summary:

         UniProtKB/Swiss-Prot: MAGA2_HUMAN, P43356
    Function: Reduces p53/TP53 transactivation function through recruitment of HDAC3 to p53/TP53 transcription sites. Also
    represses p73/TP73 activity. Proposed to enhance ubiquitin ligase activity of RING-type zinc finger-containing E3
    ubiquitin-protein ligases. In vitro enhances ubiquitin ligase activity of TRIM28 and stimulates p53/TP53
    ubiquitination by TRIM28 potentially in presence of Ubl-conjugating enzyme UBE2H. Proposed to act through recruitment
    and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. May play a role in embryonal
    development and tumor transformation or aspects of tumor progression. In vitro promotes cell viability in melanoma
    cell lines. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes

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    Gene Ontology (GO): 4 molecular function terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005515protein binding IPI--
    GO:0031625ubiquitin protein ligase binding IDA--
    GO:0042826histone deacetylase binding IDA16847267
    GO:0070491repressing transcription factor binding IDA16847267


    MAGEA2B for ontologies           About GeneDecksing



    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section



    Interactions:

        Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for MAGEA2B

    STRING Interaction Network Preview (showing 4 interactants - click image to see more details)

    5 Interacting proteins for MAGEA2B (P433561, 3 ENSP000003334874) via UniProtKB, MINT, STRING, and/or I2D
    InteractantInteraction Details
    GeneCardExternal ID(s)
    TP53P046371, 3, ENSP000002693054EBI-5650739,EBI-366083 I2D: score=1 STRING: ENSP00000269305
    HDAC3O153791, 3, ENSP000003029674EBI-5650739,EBI-607682 I2D: score=1 STRING: ENSP00000302967
    TRIM28Q132631, ENSP000002530244EBI-5650739,EBI-78139 STRING: ENSP00000253024
    UBE2HP622561EBI-5650739,EBI-2129909
    UBCENSP000003448184STRING: ENSP00000344818
    About this table

    Gene Ontology (GO): 5 biological process terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000122negative regulation of transcription from RNA polymerase II promoter IDA16847267
    GO:0006351transcription, DNA-dependent IEA--
    GO:0044257cellular protein catabolic process IMP--
    GO:0051443positive regulation of ubiquitin-protein ligase activity IMP--
    GO:0072331signal transduction by p53 class mediator IDA16847267


    MAGEA2B for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section
    Browse Small Molecules at EMD Millipore
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    Search CenterWatch for drugs/clinical trials and news about MAGEA2B / MAGA2 

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
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    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for MAGEA2B gene: 
    NM_153488.3  

    Unigene Cluster for MAGEA2B:

    Melanoma antigen family A, 2B
    Hs.534597  [show with all ESTs]
    Unigene Representative Sequence: BC108720
    11 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000492402 ENST00000370293(uc022chg.1) ENST00000423993 ENST00000447530
    ENST00000458057 ENST00000422085 ENST00000497578 ENST00000453150 ENST00000409560
    ENST00000467743 ENST00000331220(uc004ffx.1 uc004ffy.1 uc004ffz.1 uc010ntm.1 uc004fga.1)


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    Additional cDNA sequence: 

    AK290443.1 BC013098.2 BC063681.1 BC108720.1 BC109188.1 BC112158.1 

    11 DOTS entries:

    DT.95248471  DT.100815016  DT.100815017  DT.101983920  DT.97848398  DT.121277723  DT.121281043  DT.91772022 
    DT.121277711  DT.121281045  DT.92011980 

    24/183 AceView cDNA sequences (see all 183):

    BE901056 BM999965 BI090658 CA429655 NM_175742 AA905896 BM781931 BQ226610 
    CR594285 BX106621 BF219695 BX505148 BE279401 BC011744 AW673548 BU622519 
    CA488474 BQ431490 NM_175743 BM479202 NM_153488 BG575302 CA441190 BC003408 

    GeneLoc Exon Structure


    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    MAGEA2B expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: ATACAAGGAA

    Microarray
    RNAseq (Illumina Body Map)
    (100×FPKM)½
    SAGE (Serial Analysis of Gene Expression)

    About this image
    See MAGEA2B Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for MAGEA2B

    SOURCE GeneReport for Unigene cluster: Hs.534597

    UniProtKB/Swiss-Prot: MAGA2_HUMAN, P43356
    Tissue specificity: Expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma,
    lung carcinoma and breast carcinoma, but not in normal tissues except for testes

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    In Situ
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    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of animals.

    Orthologs for MAGEA2B gene from 3/14 species (see all 14)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    lizard
    (Anolis carolinensis)
    Reptilia --
    --
    32(a)
    1 → many
    1(205669616-205678308)
    zebrafish
    (Danio rerio)
    Actinopterygii ndnl26
    necdin-like 2
    23(a)
    1 → many
    23(13012414-13025121)
    fruit fly
    (Drosophila melanogaster)
    Insecta MAGE6
    MAGE
    19(a)
    1 → many
    3R(2979960-2980898)


    ENSEMBL Gene Tree for MAGEA2B (if available)
    TreeFam Gene Tree for MAGEA2B (if available) 

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for MAGEA2B gene
    MAGEA21 2  MAGEC22  MAGEA9B2  MAGEB52  MAGEB162  MAGEB42  MAGEB12  MAGEC12  
    MAGED42  MAGEA82  MAGED22  MAGEA122  MAGEA92  MAGEB172  MAGEB102  MAGEA62  
    MAGEE12  MAGED12  MAGEC32  MAGEA32  NDN2  MAGEB22  MAGEB32  MAGED4B2  
    MAGEE22  MAGEA42  MAGEA12  MAGEA102  MAGEB182  MAGEB62  MAGEL22  TRO2  
    MAGEA112  NDNL22  MAGEF12  
    18/35 SIMAP similar genes for MAGEA2B using alignment to 9 protein entries:     MAGA2_HUMAN (see all proteins) (see all similar genes):
    MAGEA2    MAGEA12    MAGEA3    MAGEA6    MAGEA5    MAGEA4
    MAGEA8    MAGEC4    MAGEA11    MAGEA1    MAGEA9    MAGEA9B
    MAGEC3    MAGEA13P    TRO    MAGEB16    MAGEB4    MAGEB5

    MAGEA2B for paralogs           About GeneDecksing



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    4 NCBI SNPs in MAGEA2B are shown     About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr X posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs2015329931,2
    --151886087(+) GGCATC/TGAGGT 2 I syn10--------
    rs2004594731,2
    ----151886336(+) CTCATC/GCAAGA 2 I M mis10--------
    rs2022319431,2
    ----151886335(+) GCTCAC/TGCAAG 2 T M mis10--------
    rs2011462481,2
    ----151886343(+) AAGATC/TTGGTG 2 L syn10--------

    HapMap Linkage Disequilibrium report for MAGEA2B (151883082 - 151887096 bp)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
    Database of Genomic Variants (DGV): 1 variation for MAGEA2B
         1 CNV: 3273

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    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    MAGEA2B for disorders           About GeneDecksing

    OMIM gene information: 300549    OMIM disorders: --

    7 diseases for MAGEA2B:    About MalaCards
    dyskeratosis congenita    melanoma    dyskeratosis    squamous cell carcinoma
    lung carcinoma    breast carcinoma    carcinoma


    Export disorders for MAGEA2B gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for MAGEA2B gene, integrated from 9 sources (see all 15):
    (articles sorted by number of sources associating them with MAGEA2B)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. MAGE-RING protein complexes comprise a family of E3 u biquitin ligases. (PubMed id 20864041)1, 2 Doyle J.M....Potts P.R. (2010)
    2. MAGE-A tumor antigens target p53 transactivation function through histone deacetylase recruitment and confer resistance to chemotherapeutic agents. (PubMed id 16847267)1, 2 Monte M....Schneider C. (2006)
    3. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
    4. Comparative genome sequence analysis of the Bpa/Str region in mouse and man. (PubMed id 10854409)1, 2 Mallon A.-M....Brown S.D.M. (2000)
    5. The melanoma antigen gene (MAGE) family is clustered in the chromosomal band Xq28. (PubMed id 8575766)1, 3 Rogner U.C....Poustka A. (1995)
    6. Human gene MAGE-3 codes for an antigen recognized on a melanoma by autologous cytolytic T lymphocytes. (PubMed id 8113684)1, 2 Gaugler B.... Boon T. (1994)
    7. Sequence and expression pattern of the human MAGE2 gene. (PubMed id 8276455)1, 2 De Smet C....Boon T. (1994)
    8. Ubiquitin ligase substrate identification through qua ntitative proteomics at both the protein and peptide levels. (PubMed id 21987572)1 Lee K.A....Doedens J.R. (2011)
    9. Global identification of modular cullin-RING ligase su bstrates. (PubMed id 21963094)1 Emanuele M.J....Elledge S.J. (2011)
    10. MAGE-A, mMage-b, and MAGE-C proteins form complexes with KAP1 and suppress p53-dependent apoptosis in MAGE-positive cell lines. (PubMed id 17942928)2 Yang B.... Longley B.J. (2007)

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    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Entrez Gene: 266740 HGNC: 19340 AceView: MAGEA2andMAGEA3andMAGEA12 Ensembl:ENSG00000183305 euGenes: HUgn266740
    ECgene: MAGEA2B H-InvDB: MAGEA2B

    (According to HUGE)
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