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MAGEA12 Gene

protein-coding GIFtS: 51

GC0XM151649

melanoma antigen family A, 12
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
(Previous symbol: MAGE12)

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc , and/or ⁷Ensembl, ⁸miRBase)
About This Section

Aliases
CT1.12 ^¹, ², ³
MAGE12 ^², ³, ⁵
OTTHUMP00000024231 ^²

Descriptions
Cancer/testis antigen 1.12 ^³
MAGE-12 antigen ^³
MAGE12F antigen ^³
cancer/testis antigen family 1, member 12 ^²
melanoma antigen family A, 12 ^²

External Ids
HGNC: 6799¹
Entrez Gene: 4111²
UniProtKB: P43365³
Ensembl: ENSG00000197172⁷

Search outside databases for aliases for MAGEA12 gene

Previous GC identifers: GC0XM146239 GC0XM148556 GC0XM149647 GC0XM150516 GC0XM151476 GC0XM151628

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for MAGEA12:

This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50

to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show

considerable variability, suggesting that the existence of this gene family enables the same

function to be expressed under different transcriptional controls. The MAGEA genes are clustered

at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as

dyskeratosis congenita. [provided by RefSeq]

UniProtKB/Swiss-Prot: MAGAC_HUMAN, P43365

Function: Not known, though may play a role in tumor transformation or aspects of tumor progression

Gene Wiki entry for MAGEA12

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:

UCSC Golden Path with GeneCards custom track

Transcription factor binding sites upstream to the MAGEA12 gene

Entrez Gene cytogenetic band: Xq28 Ensembl cytogenetic band: Xq28 HGNC cytogenetic band: Xq28

MAGEA12 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome X GeneLoc Exon Structure

GeneLoc location for GC0XM151649: (about GC identifiers)

Start:	151,649,949 bp from pter
End:	151,653,831 bp from pter
Size:	3,883 bases
Orientation:	minus strand

RefSeq DNA sequence:

NC_000023.9 NT_011726.13

(According to ¹UniProtKB, and/or Ensembl, Phosphorylation sites according to ²Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: MAGAC_HUMAN, P43365 (See protein sequence)

Recommended Name: Melanoma-associated antigen 12

Size: 314 amino acids; 34836 Da

Secondary accessions: Q9NSD3

REFSEQ proteins: NP_005358.2

ENSEMBL proteins:

ENSP00000350592

Human Recombinant Proteins

	Browse Drug Discovery Central at Invitrogen for human recombinant proteins
	Browse Purified and Recombinant Proteins at Millipore
	Browse Human Recombinant Proteins at Sigma-Aldrich
	Browse R&D Systems for human recombinant proteins
	Browse recombinant and purified proteins available from Enzo Life Sciences
	Recombinant Proteins from Abcam (MAGEA12)
	Human Recombinant Proteins from Abnova (MAGEA12)

Browse Origene for full length recombinant human proteins expressed in human HEK293 cells

1 Gene Ontology (GO) cellular component term (links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005575	cellular_component	ND	--

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Antibodies for MAGEA12:

	Browse Antibodies Central at Invitrogen
	Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
	Browse Antibodies at Sigma-Aldrich
	Browse R&D Systems for Antibodies
	Antibodies from Abcam (MAGEA12), each with their Abpromise^SM
	Monoclonal and Polyclonal Antibodies from Abnova (MAGEA12)
	Novus Biologicals Antibodies for MAGEA12

Assays for MAGEA12:

	Browse Invitrogen for biochemical assays
	Browse Kits and Assays available from Millipore
	Browse R&D Systems for biochemical assays
	Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

Graphical View of Domain Structure for InterPro Entry P43365

ProtoNet protein and cluster: P43365

1 Blocks protein family: IPB002190 MAGE protein

UniProtKB/Swiss-Prot: MAGAC_HUMAN, P43365

Similarity: Contains 1 MAGE domain

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

Inhib. RNA:		Invitrogen RNAi Products for gene knock-down (MAGEA12)
		Browse for Gene Knock-down Tools from Millipore
		Abnova Chimera RNAi Products for Gene knock-down (MAGEA12)

OriGene 29mer shRNA kit in GFP-retroviral vector: NM_005367

Applied Biosystems Silencer® siRNAs for MAGEA12

Sigma-Aldrich siRNA for MAGEA12

Sigma-Aldrich shRNA for MAGEA12

Explore Sigma-Aldrich super-pooled esiRNAs

Clones:		Invitrogen Clones for MAGEA12
		Browse Clones for the Expression of Recombinant Proteins Available from Millipore

OriGene GFP tagged cDNA clone in CMV expression vector: NM_005367
Myc/DDK tagged cDNA clone in CMV expression vector: NM_005367
untagged cDNA clone in CMV expression vector: NM_005367

Primers:

Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers

OriGene genome-wide validated SYBR primer pairs: NM_005367

UniProtKB/Swiss-Prot: MAGAC_HUMAN, P43365

Function: Not known, though may play a role in tumor transformation or aspects of tumor progression

Genatlas biochemistry entry for MAGEA12:

human melanoma antigen,family A,member 12,expressed in melanoma,testis

1 Gene Ontology (GO) molecular function term (links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0003674	molecular_function	ND	--

About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to ¹UniProtKB, ²MINT, and/or ³STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

1 Interacting protein for MAGEA12 (P43365²) via UniProtKB, MINT, and/or STRING
Interactant Interaction Details
GeneCard External ID(s)
STAT5A P42229² MINT-67540
About this table
1 Gene Ontology (GO) biological process term (links to tree view):
GO ID Qualified GO term Evidence PubMed IDs
GO:0008150 biological_process ND --
About this table

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences

Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for MAGEA12

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib. RNA:		Invitrogen RNAi Products for gene knock-down (MAGEA12)
		Browse for Gene Knock-down Tools from Millipore
		Abnova Chimera RNAi Products for Gene knock-down (MAGEA12)

OriGene 29mer shRNA kit in GFP-retroviral vector: NM_005367

Sigma-Aldrich siRNA for MAGEA12

Sigma-Aldrich shRNA for MAGEA12

Explore Sigma-Aldrich super-pooled esiRNAs

Applied Biosystems Silencer® siRNAs:

NM_005367

REFSEQ mRNAs for MAGEA12 gene:

NM_005367.4

Applied Biosystems TaqMan ® Gene Expression Assays:

NM_005367

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_005367
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_005367
                                 untagged cDNA clone in CMV expression vector: NM_005367

Additional cDNA sequence:

AK314168.1 BC003408.2 BC098146.1 BT007108.1 CR541775.1 CR594285.1

4 DOTS entries:

DT.441466 DT.95248475 DT.91772013 DT.95280682

24/183 AceView cDNA sequences (see all 183 ):

NM_005362 BC005963 BX505148 BC063681 BF219695 BM781931 CA415709 BM449726

BC000340 CR594285 AW628104 BE279401 BF791772 AI222439 BM762699 NM_175742

BU182773 BQ226610 BG575302 BX106621 CD557117 BM479202 BQ431490 BM015882

highest scoring ESTs for MAGEA12:

L18877 BC003408 BE542433 BE795782 BE890732 BE895222 BM015975 BM479202 BM846958 BM847865

Unigene Cluster for MAGEA12:

Melanoma antigen family A, 12

Hs.169246 [show with all ESTs]

Unigene Representative Sequence: NM_005367

GeneLoc Exon Structure

1 Ensembl transcript including schematic representation:

ENST00000357916

(Experimental results according to ¹GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to ²GeneAnnot , ³GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

MAGEA12 expression in normal and diseased human tissues

Applied Biosystems TaqMan ® Gene Expression Assays for MAGEA12

¹ / ² / ³

3 probe-sets matching MAGEA12 gene

Affymetrix probe-set	Array	# genes	Sensitivity	Specificity	Correlation	Length	Gb_Accession	Consensus	Uniqueness	Score	Rank
Affymetrix probe-set	Array	GeneAnnot data			GeneNote data		GeneTide data
31480_f_at²^{, 3}	U95-A	11	1.00	0.32	1.00	1.00	L18877	0.20	1.00	0.72	1
210467_x_at²^{, 3}	U133-A	10	1.00	0.35	--	--	BC003408	0.60	0.62	0.61	1
210467_x_at²	U133Plus2	10	1.00	0.35	--	--	--	--	--	--	--

About this table

Data from

(Publications) and GNF BioGPS
About these images
About these images

CGAP SAGE TAG: GATACCTGGA

SOURCE GeneReport for Unigene cluster: Hs.169246
UniProtKB/Swiss-Prot: MAGAC_HUMAN, P43365
Tissue specificity: Expressed in many tumors of several types, such as melanoma, head and neck
squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for
testes

(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD and/or ⁵MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section

Orthologs for MAGEA12 gene from 1 species

Organism	Gene	Locus	Description	Human Similarity	NCBI accessions
chimpanzee (Pan troglodytes)	MAGEA12¹	--	melanoma antigen family A, 12	99.04(n) 98.73(a)	465915 XM_521314.2 XP_521314.2

About this table Species with no ortholog for MAGEA12

ENSEMBL Gene Tree for MAGEA12

(Paralogs according to ¹HomoloGene
and ²Ensembl, Pseudogenes according to ³Pseudogene.org)
About This Section

Paralogs for MAGEA12 gene

MAGEA3² MAGEA2B² MAGEA8² MAGEA9² MAGEA9B² MAGEA4² MAGEA11² MAGEA1²

MAGEA10² MAGEA6² MAGEA2²

(According to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section

10/15 NCBI SNPs in MAGEA12 are shown (see all 15 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay) (see all 12)

AB	SNP ID	Valid	Chr X pos	Sequence	Recs	AA Chg	Type	More	Recs	Allele freq	Pop	Total sample	More
AB	Genomic Data				Transcription Data				Allele Frequencies
				--	--	--		--	--				--
--	rs2471773^1,2	A,C,F,H,O	151650011(+)	`ATCCAC/TGCTTT`	1	--	ut3¹		11		EU EA WA NA	766
--	rs2471772^1,2	A,C,F,H,O	151649731(+)	`AACTCT/CCTCCT`	1	--	ng5¹		4		EU NA	252
--	rs3005^1,2	A,C,F	151650445(-)	`GGCCCC/TATCCA`	1	--	ut3¹		4		NA EA	216
--	rs16996512^1,2	C,F	151651286(+)	`GGTGAC/GTCGGC`	1	E/D	mis¹		7		NA EU EA WA	352
--	rs2515830^1,2	C	151650416(+)	`CTCATA/GTCACA`	1	--	ut3¹		0	--	--	--	--
--	rs2233056^1,2	A	151650474(-)	`GAGGGA/GGTCTG`	1	--	ut3¹		0	--	--	--	--
--	rs16996510^1,2	F	151651512(+)	`GCTCCG/AGGGCC`	1	--	ut5¹		3		NA	142
--	rs1894358^1,2	F,H	151651283(+)	`CTTGGT/GGACTC`	1	S/S	syn¹		8		NA EA EU	534
--	rs2233055^1,2	O	151650569(-)	`GGAGGA/GCCTCA`	1	G/G	syn¹		0	--	--	--	--
--	rs72182325^1,2	--	151650616(+)	`GCTGG-/TTTCAA`	1	T/	fra¹		0	--	--	--	--

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HapMap Linkage Disequilibrium images for MAGEA12 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 300177

(Possibly Related Articles in Doctor's Guide)
About This Section

(in PubMed. Associations of this gene to articles via ¹Novoseek, ²HGNC, ³Entrez Gene, ⁴UniProtKB/Swiss-Prot, ⁵UniProtKB/TrEMBL, ⁶GAD, and/or ⁷PharmGKB)
About This Section

10 PubMed articles for MAGEA12 gene:

The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)^{3, 4} Gerhard D.S....Malek J. (2004)
Comparative genome sequence analysis of the Bpa/Str region in mouse and Man. (PubMed id 10854409)^{3, 4} Mallon A.M....Brown S.D. (2000)
The melanoma antigen gene (MAGE) family is clustered in the chromosomal band Xq28. (PubMed id 8575766)^{2, 3} Rogner U.C....Poustka A. (1995)
Cloning and analysis of MAGE-1-related genes. (PubMed id 8037761)^{3, 4} Ding M.... Fenton R.G. (1994)
Sequence and expression pattern of the human MAGE2 gene. (PubMed id 8276455)^{3, 4} De Smet C....Boon T. (1994)
Expression of melanoma-associated antigens in oral squamous cell carcinoma. (PubMed id 18197853)³ Ries J....Nkenke E. (2008)
Methyl-CpG binding domain proteins and their involvement in the regulation of the MAGE-A1, MAGE-A2, MAGE-A3, and MAGE-A12 gene promoters. (PubMed id 17634428)³ Wischnewski F....Schwarzenbach H. (2007)
Towards a proteome-scale map of the human protein-protein interaction network. (PubMed id 16189514)³ Rual J.F....Vidal M. (2005)
Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)³ Strausberg R.L....Marra M.A. (2002)
Structure, chromosomal localization, and expression of 12 genes of the MAGE family. (PubMed id 7927540)³ De Plaen E.... Boon T. (1994)

(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

		AND	OR
Aliases
Free Text

Query String		PubMed OMIM NCBI Bookshelf
	(Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 4111	HGNC: 6799	AceView: MAGEA2andMAGEA3andMAGEA12	Ensembl:ENSG00000197172	euGenes: HUgn4111
ECgene: MAGEA12	H-InvDB: MAGEA12

(According to HUGE)
About This Section

(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

Name	Description
ATLAS Chromosomes in Cancer entry for MAGEA12	Genetics and Cytogenetics in Oncology and Haematology

Licensable Technologies for MAGEA12
(Available from WIS Yeda, Salk, Tufts)

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(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section

Products for MAGEA12:

TaqMan ® Gene Expression Assays

TaqMan ® Genotyping Assays

Free SNP selection tool

Invitrogen iPath Pathways	Invitrogen BLOCK-iT™ RNAi
Invitrogen Antibodies	Invitrogen Assays
Invitrogen Clones	Invitrogen Q-PCR Products
Invitrogen Human Recombinant Kinases	Invitrogen Custom Antibody and Peptide Service
Invitrogen Proteins / Assays / Screening Services	Search Invitrogen catalog for MAGEA12-related products

	Millipore Custom Antibody & Bulk Services
	Millipore Preclinical / Clinical Development Services
	Millipore Immunoassay Services
	Millipore Target Screening & Profiling Services

	Predesigned and custom siRNAs for MAGEA12		Browse antibodies at Sigma-Aldrich
	Explore super-pooled esiRNAs		Browse proteins at Sigma-Aldrich
	Lentivirus-delivered shRNAs for MAGEA12		Browse small molecules at Sigma-Aldrich
	"Your Favorite Gene" Pathways		feedback

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	Cell Culture Products		ELISAs
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	Multiplex/Array Assay Kits & Reagents		ELISpot Kits & Development Modules
	Recombinant & Natural Proteins

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MAGEA12 Gene

protein-coding GIFtS: 51

Aliases &
Descriptions
for MAGEA12

Summaries
for MAGEA12

Genomic Location
for MAGEA12

Proteins
for MAGEA12

Protein Domains/
Families
for MAGEA12

Gene Function
for MAGEA12

Pathways & Interactions
for MAGEA12

Drugs & Compounds
for MAGEA12

Transcripts
for MAGEA12

Expression
for MAGEA12

Orthologs
for MAGEA12

Paralogs
for MAGEA12

SNPs/Variants
for MAGEA12

Disorders & Mutations
for MAGEA12

Medical News
for MAGEA12

Publications
for MAGEA12

Search for MAGEA12

Genome Databases showing MAGEA12

Other Databases showing MAGEA12

Specialized Databases showing MAGEA12

Licensable Technologies
for MAGEA12

Services
for MAGEA12

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

MAGEA12 Gene

protein-coding GIFtS: 51

Aliases & Descriptionsfor MAGEA12

Summariesfor MAGEA12

Genomic Locationfor MAGEA12

Proteinsfor MAGEA12

Protein Domains/Familiesfor MAGEA12

Gene Functionfor MAGEA12

Pathways & Interactionsfor MAGEA12

Drugs & Compoundsfor MAGEA12

Transcriptsfor MAGEA12

Expression for MAGEA12

Orthologsfor MAGEA12

Paralogsfor MAGEA12

SNPs/Variantsfor MAGEA12

Disorders & Mutationsfor MAGEA12

Medical Newsfor MAGEA12

Publicationsfor MAGEA12

Search for MAGEA12

Genome Databases showing MAGEA12

Other Databases showing MAGEA12

Specialized Databases showing MAGEA12

Licensable Technologiesfor MAGEA12

Servicesfor MAGEA12

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

Aliases &
Descriptions
for MAGEA12

Summaries
for MAGEA12

Genomic Location
for MAGEA12

Proteins
for MAGEA12

Protein Domains/
Families
for MAGEA12

Gene Function
for MAGEA12

Pathways & Interactions
for MAGEA12

Drugs & Compounds
for MAGEA12

Transcripts
for MAGEA12

Expression
for MAGEA12

Orthologs
for MAGEA12

Paralogs
for MAGEA12

SNPs/Variants
for MAGEA12

Disorders & Mutations
for MAGEA12

Medical News
for MAGEA12

Publications
for MAGEA12

Licensable Technologies
for MAGEA12

Services
for MAGEA12