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MAF1 Gene

protein-coding   GIFtS: 54

GC08P145231
MAF1 homolog (S. cerevisiae)
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases
DKFZp586G1123 1
MGC20332 2
MGC31779 2
MGC39758 2
Descriptions
MAF1 homolog (S. cerevisiae) 2
MAF1 protein 2
homolog of yeast MAF1 2
External Ids
HGNC: 249661
Entrez Gene: 842322
UniProtKB: Q9H0633
Ensembl: ENSG000001796327
Search outside databases for aliases for MAF1 gene

Previous GC identifers: GC00U990992 GC08M145276 GC08P144934 GC08P145265

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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EntrezGene summary for MAF1:
This gene encodes a protein that is similar to Maf1, a Saccharomyces cerevisiae protein highly
conserved in eukaryotic cells. Yeast Maf1 is a negative effector of RNA polymerase III (Pol III).
It responds to changes in the cellular environment and represses pol III transcription.
Biochemical studies identified the initiation factor TFIIIB as a target for Maf1-dependent
repression. [provided by RefSeq]

UniProtKB/Swiss-Prot: MAF1_HUMAN, Q9H063
Function: Mediator of diverse signals that repress RNA polymerase III transcription. Inhibits the
de novo assembly of TFIIIB onto DNA (By similarity)

Gene Wiki entry for MAF1

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the MAF1 gene  

Entrez Gene cytogenetic band: 8q24.3   Ensembl cytogenetic band:  8q24.3   HGNC cytogenetic band: 8q24.3

MAF1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 8         GeneLoc Exon Structure

GeneLoc location for GC08P145231:     (about GC identifiers)

Start:
145,231,293 bp from pter
End:
145,234,503 bp from pter
Size:
3,211 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000008.9  NT_023684.17  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: MAF1_HUMAN, Q9H063 (See protein sequence)
Recommended Name: Repressor of RNA polymerase III transcription MAF1 homolog  
Size: 256 amino acids; 28870 Da
Subunit: Interacts with BRF2
Subcellular location: Nucleus (By similarity)

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_115648.2  

    ENSEMBL proteins: 
    ENSP00000318604 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (MAF1)
    Human Recombinant Proteins from Abnova (MAF1)
                  OriGene Purified Recombinant Human Protein: MAF1 

    2 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005622 intracellular IDA11256614
    GO:0005634 nucleus IEA--
    About this table

    Antibodies for MAF1: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Browse Antibodies at Sigma-Aldrich
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (MAF1), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (MAF1)
    Search Novus for antibodies for MAF1

    Assays for MAF1: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    2 InterPro domains/families:
     IPR015257 Maf1
     IPR017152 RNA_pol_III_tscrpt_repres_MAF1


       GeneDecks  MAF1 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q9H063

    ProtoNet protein and cluster: Q9H063

    UniProtKB/Swiss-Prot: MAF1_HUMAN, Q9H063
    Similarity: Belongs to the MAF1 family

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (MAF1)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_032272

                  Applied Biosystems Silencer® siRNAs for MAF1

                  Sigma-Aldrich siRNA for MAF1  
                         Sigma-Aldrich shRNA for MAF1  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for MAF1
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_032272
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_032272
                                     untagged cDNA clone in CMV expression vector: NM_032272 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_032272

    UniProtKB/Swiss-Prot: MAF1_HUMAN, Q9H063
    Function: Mediator of diverse signals that repress RNA polymerase III transcription. Inhibits the
    de novo assembly of TFIIIB onto DNA (By similarity)

    1 Gene Ontology (GO) molecular function term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0030528 transcription regulator activity IEA--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


     Gene Network CentralTM Interacting Genes and Proteins Network for  MAF1 


    5/6 Interacting proteins for MAF1 (Q9H0632 ENSP000003186043) via UniProtKB, MINT, and/or STRING (see all 6 )
    InteractantInteraction Details
    GeneCardExternal ID(s)
    HBXIPO435042MINT-63154
    SUMO2P619562MINT-65173
    TBPENSP000002303543STRING (score=.872)
    MAFENSP000003270483STRING (score=.738)
    MAFBENSP000003624103STRING (score=.732)
    About this table

    3 Gene Ontology (GO) biological process terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006350 transcription IEA--
    GO:0006355 regulation of transcription, DNA-dependent IEA--
    GO:0016480 negative regulation of transcription from RNA polymerase III promoter IEA--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for MAF1

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (MAF1)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_032272

                  Sigma-Aldrich siRNA for MAF1  
                         Sigma-Aldrich shRNA for MAF1  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_032272  

    REFSEQ mRNAs for MAF1 gene: 

    NM_032272.4   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_032272  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_032272
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_032272
                                     untagged cDNA clone in CMV expression vector: NM_032272 

    Additional cDNA sequence: 

    AL136937.1 BC014082.2 BC018714.1 BC031273.1 CR533463.1 CR600048.1 CR621223.1 

    16 DOTS entries:

    DT.456226  DT.95370014  DT.97855651  DT.92433869  DT.95370026  DT.86854204  DT.95370023  DT.100059461 
    DT.92447789  DT.95370020  DT.95370021  DT.100653825  DT.100694224  DT.100847506  DT.97792575  DT.100834273 

    24/509 AceView cDNA sequences (see all 509 ):

    AI494521 BG314753 BU164016 BM705477 BE222230 BM789545 BQ086344 AI701046 
    BM832303 BM729354 AA477528 CR621223 AA056632 BU730617 BM762763 BX490677 
    AI817120 BE677318 CR600048 BM850571 BF434181 AW614830 BM993066 AA600986 

    highest scoring ESTs for MAF1:

    AA002187 AA022753 AA022864 AA036928 AA135841 AA143586 AA143587 AA155652 AA158793 AA282921 

    Unigene Cluster for MAF1:

    MAF1 homolog (S. cerevisiae)
    Hs.19673  [show with all ESTs]
    Unigene Representative Sequence: NM_032272


    GeneLoc Exon Structure

    5 Alternative Splicing Database (ASD) splice patterns (SP) for MAF1

    ExUns: 1 ^ 2a · 2b · 2c ^ 3 ^ 4 ^ 5a · 5b ^ 6 ^ 7a · 7b ^ 8a · 8b ^ 9a · 9b
    SP1:        -     -     -                                                                     
    SP2:                    -                                                                     
    SP3:        -     -     -                                                                     
    SP4:                                                                                          
    SP5:                                                                                          

    About this scheme

    ECgene alternative splicing isoforms for MAF1

    1 Ensembl transcript including schematic representation:
    ENST00000322428  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    MAF1 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for MAF1

    1 / 2 / 3

    3 probe-sets matching MAF1 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

    57059_at2, 3 U95-B 1 1.00 1.00 1.00 1.00 AA889576 0.80 1.00 0.91 1

    222998_at2, 3 U133-B 1 1.00 1.00 -- -- AL136937 0.80 1.00 0.91 1

    222998_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    About this table
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: CTGTGGCCGG

    SOURCE GeneReport for Unigene cluster: Hs.19673
    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for MAF1 gene from 5/19 species (see all 19 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    MAF11   -- MAF1 homolog (S. cerevisiae) 90.1(n)
    96.09(a)
    482087  XM_539208.2  XP_539208.2 
    chimpanzee
    (Pan troglodytes)
    MAF11   -- MAF1 homolog (S. cerevisiae) 99.22(n)
    100(a)
    464456  XM_001155873.1  XP_001155873.1 
    cow
    (Bos taurus)
    MAF11   -- MAF1 homolog (S. cerevisiae) 90.94(n)
    99.03(a)
    512498  NM_001034424.1  NP_001029596.1 
    rat
    (Rattus norvegicus)
    Maf11   -- MAF1 homolog (S. cerevisiae) 87.11(n)
    95.31(a)
    315093  NM_001014085.1  NP_001014107.1 
    mouse
    (Mus musculus)
    Maf11, 5 155
    MAF1 homolog (S. cerevisiae)1, 5 87.11(n)1
    94.14(a)1
    688771  NM_026859.21  NP_081135.21 
     AI8417715  AK0044065  (see all 17)
    About this table        Species with no ortholog for MAF1

    ENSEMBL Gene Tree for MAF1
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Pseudogenes
    1 related pseudogene


    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10 NCBI SNPs in MAF1 are shown
    (Click for Applied Biosystems TaqMan ® Genotyping Assay) (only AB snps)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 8 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs100914011,2
    A,C,F,H145234586(+) GCTGCC/GGGCCG 1 -- ng314Minor allele frequency- G:0.13EU EA WA 418
    rs115461441,2
    C,F145233811(+) GAAGCA/GGGGGC 1 G/R mis15Minor allele frequency- G:0.04NA EU EA WA 480
    --
    rs66851,2
    A145234360(-) TGCTCA/GTGGGG 1 -- ut31 ese30--------
    rs132633421,2
    A145233884(+) GGTCCC/TTGAAG 1 -- int10--------
    --
    rs723748761,2
    --145234538(+) CTGCG-/TGATGCGGTGTG
    GGGGTGGGGCCTGC
    GTGTG
    1 -- ng310--------
    --
    rs115461431,2
    --145234245(-) GGCAGC/TCGCTC 1 -- ut31 ese30--------
    --
    rs733813111,2
    --145234273(+) CACAGA/CAGGGA 1 -- ut310--------
    --
    rs351105631,2
    --145234603(+) TAAGT-/ACCTGG 1 -- ng310--------
    --
    rs574336241,2
    --145233786(+) GAGGAA/GGAGGT 1 E/E syn10--------
    --
    rs359534131,2
    --145233877(+) GACCC-/CGGGTC 1 -- int10--------
    About this table
    The following additional 47 SNP(s) have been found in Applied Biosystems data source:
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)


     rs35973351 rs35387134 rs35013727 rs12543080 rs35029296 rs13281660 rs28398315
     rs7836836 rs34960192 rs4977196 rs4075064 rs35514433 rs34755830 rs13254954
     rs11136256 rs59976468 rs35671863 rs2070688 rs1126919 rs35351724 rs4977193
     rs4075389 rs34324679 rs34281202 rs61745189 rs61745399 rs35801151 rs1132230
     rs4977194 rs6558301 rs4977195 rs1071599 rs3211523 rs12550729 rs34173062
     rs34839093 rs11541804 rs34948990 rs34580334 rs55908964 rs35397593 rs34674752
     rs34079855 rs34079907 rs34728076 rs7829844 rs35844464

    HapMap Linkage Disequilibrium images for MAF1 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 610210

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/16 PubMed articles for MAF1 gene (see all 16 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 84232 HGNC: 24966 AceView: MAF1 Ensembl:ENSG00000179632 euGenes: HUgn84232
    ECgene: MAF1 H-InvDB: MAF1
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for MAF1:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



      Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
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     Predesigned and custom siRNAs for MAF1 Browse antibodies at Sigma-Aldrich
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for MAF1 Browse small molecules at Sigma-Aldrich
     "Your Favorite Gene" Pathwaysfeedback


      
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     Recombinant & Natural Proteins  

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     Custom Service for Rabbit Pab from Full-length Protein
      
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     Recombinant Proteins
    (MAF1)
     Antibodies (MAF1)
     Tagged/untagged cDNA clones
     Validated SYBR primer pairs
     Purified proteins from HEK293T cells
     Western blot validated antibodies
     shRNA in GFP-retroviral vector
     Search Tocris compounds for MAF1




     Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
     Search Novus for antibodies for MAF1

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