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MAEA Gene

protein-coding   GIFtS: 53

GC04P001273
macrophage erythroblast attacher
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases
EMLP 2
EMP 1, 2, 3, 5
HLC-10 2, 3
PIG5 2
Descriptions
Cell proliferation-inducing gene 5 protein 3
Erythroblast macrophage protein 2, 3
Human lung cancer oncogene 10 protein 3
lung cancer-related protein 10 2
macrophage erythroblast attacher 2
proliferation-inducing gene 5 2
External Ids
HGNC: 137311
Entrez Gene: 102962
UniProtKB: Q7L5Y93
Ensembl: ENSG000000903167
Search outside databases for aliases for MAEA gene

Previous GC identifer: GC04P001289

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for MAEA:
This gene product mediates the attachment of erythroblasts to macrophages. This attachment promotes
terminal maturation and enucleation of erythroblasts, presumably by suppressing apoptosis. This
protein is an integral membrane protein with the N-terminus on the extracellular side and the
C-terminus on the cytoplasmic side of the cell. Two immunologically related isoforms of
erythroblast macrophage protein with apparent molecular weights of 33 kD and 36 kD were detected
in macrophage membranes; this gene encodes the larger isoform. Multiple alternatively spliced
transcript variants encoding distinct isoforms have been found for this gene, but the biological
validity of some variants has not been determined. [provided by RefSeq]

UniProtKB/Swiss-Prot: MAEA_HUMAN, Q7L5Y9
Function: Play a role in erythroblast enucleation and in the development of the mature macrophages.
Mediates the attachment of erythroid cell to mature macrophages, in correlation with the presence
of MAEA at cell surface of mature macrophages; This MAEA-mediated contact inhibits erythroid cells
apoptosis. Participates to erythroblastic island formation, which is the functional unit of
definitive erythropoiesis. Associates with F-actin to regulate actin distribution in erythroblasts
and macrophages. May contribute to nuclear architecture and cells division events

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the MAEA gene  

Entrez Gene cytogenetic band: 4p16.3   Ensembl cytogenetic band:  4p16.3   HGNC cytogenetic band: 4p16.3

MAEA Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 4         GeneLoc Exon Structure

GeneLoc location for GC04P001273:     (about GC identifiers)

Start:
1,273,672 bp from pter
End:
1,323,925 bp from pter
Size:
50,254 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000004.10  NT_037622.5  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: MAEA_HUMAN, Q7L5Y9 (See protein sequence)
Recommended Name: Macrophage erythroblast attacher  
Size: 396 amino acids; 45287 Da
Subunit: Form a complex with F-actin
Subcellular location: Nucleus matrix. Cell membrane. Cytoplasm, cytoskeleton. Note=Localized as
nuclear speckled-like pattern
Developmental stage: Localized with condensed chromatin at prophase; Detected in nuclear spindle
poles at metaphase and in the contractile ring during telophase and cytokinesis
Sequence caution: Sequence=AAC67543.1; Type=Frameshift; Positions=252, 327;
Secondary accessions: O95285 Q5JB54 Q6ZRD6 Q9BQ11 Q9H9V6 Q9H9Z4 Q9NW84
Alternative splicing: 6 isoforms:  Q7L5Y9-1   Q7L5Y9-2   Q7L5Y9-3   Q7L5Y9-4   Q7L5Y9-5   Q7L5Y9-6   

REFSEQ proteins (2 alternative transcripts): 
NP_001017405.1  NP_005873.2  


ENSEMBL proteins: 
ENSP00000302830 ENSP00000264750 ENSP00000372405 


Human Recombinant Proteins 
Browse Drug Discovery Central at Invitrogen for human recombinant proteins
Browse Purified and Recombinant Proteins at Millipore
Browse Human Recombinant Proteins at Sigma-Aldrich  
Browse R&D Systems for human recombinant proteins
Browse recombinant and purified proteins available from Enzo Life Sciences
Recombinant Proteins from Abcam (MAEA)
Human Recombinant Proteins from Abnova (MAEA)
              OriGene Purified Recombinant Human Protein: MAEA 

5/9 Gene Ontology (GO) cellular component terms (links to tree view) (see all 9 ):

GO IDQualified GO termEvidencePubMed IDs
GO:0005624 membrane fraction IDA9763581
GO:0005634 nucleus IEA--
GO:0005737 cytoplasm IEA--
GO:0005819 spindle IDA16510120
GO:0005826 contractile ring IDA16510120
About this table

Antibodies for MAEA: 
Browse Antibodies Central at Invitrogen
Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
Browse Antibodies at Sigma-Aldrich
Browse R&D Systems for Antibodies
Antibodies from Abcam (MAEA), each with their AbpromiseSM
Browse Abnova for Monoclonal and Polyclonal Antibodies
Search Novus for antibodies for MAEA

Assays for MAEA: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

3 InterPro domains/families:
 IPR006595 CTLH_C
 IPR006594 LisH_dimerisation
 IPR013144 Ran-bd_prot-like_CRA_domain


   GeneDecks  MAEA for the domains selected above  
About GeneDecksing

Graphical View of Domain Structure for InterPro Entry Q7L5Y9

ProtoNet protein and cluster: Q7L5Y9

2 Blocks protein families:
IPB006594 Lissencephaly type-1-like homology motif
IPB006595 CTLH


UniProtKB/Swiss-Prot: MAEA_HUMAN, Q7L5Y9
Similarity: Contains 1 CTLH domain
Similarity: Contains 1 LisH domain

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (MAEA)
Browse for Gene Knock-down Tools from Millipore
Browse Abnova for Chimera RNAi Products
               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_001017405

              Applied Biosystems Silencer® siRNAs for MAEA

              Sigma-Aldrich siRNA and siRNA Panels for MAEA  
                     Sigma-Aldrich shRNA Panels and shRNA for MAEA  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Invitrogen Clones for MAEA
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
               OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_001017405
                                 Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_001017405
                                 untagged cDNA clones in CMV expression vector (see all 2): NM_001017405 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_001017405

UniProtKB/Swiss-Prot: MAEA_HUMAN, Q7L5Y9
Function: Play a role in erythroblast enucleation and in the development of the mature macrophages.
Mediates the attachment of erythroid cell to mature macrophages, in correlation with the presence
of MAEA at cell surface of mature macrophages; This MAEA-mediated contact inhibits erythroid cells
apoptosis. Participates to erythroblastic island formation, which is the functional unit of
definitive erythropoiesis. Associates with F-actin to regulate actin distribution in erythroblasts
and macrophages. May contribute to nuclear architecture and cells division events

5 MGI mutant phenotypes (inferred from 1 allele(MGI details for Maea):

growth/sizehematopoietic systemimmune systemlethality-prenatal/perinatalskin/coat/nails

1 Gene Ontology (GO) molecular function term (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0003779 actin binding IDA16510120
About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section



5/8 Interacting proteins for MAEA (ENSP000003028303) via UniProtKB, MINT, and/or STRING (see all 8 )
InteractantInteraction Details
GeneCardExternal ID(s)
BCL2ENSP000003296233STRING (score=.86)
COL1A1ENSP000002259643STRING (score=.84)
FN1ENSP000003235343STRING (score=.814)
PTGS2ENSP000003564383STRING (score=.8)
KLK3ENSP000003141513STRING (score=.744)
About this table

5/6 Gene Ontology (GO) biological process terms (links to tree view) (see all 6 ):

GO IDQualified GO termEvidencePubMed IDs
GO:0007049 cell cycle IEA--
GO:0007155 cell adhesion IDA9763581
GO:0007346 regulation of mitotic cell cycle NAS16510120
GO:0033033 negative regulation of myeloid cell apoptosis IDA9763581
GO:0043249 erythrocyte maturation IEA--
About this table
(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for MAEA

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (MAEA)
Browse for Gene Knock-down Tools from Millipore
Browse Abnova for Chimera RNAi Products
               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_001017405

              Sigma-Aldrich siRNA and siRNA Panels for MAEA  
                     Sigma-Aldrich shRNA Panels and shRNA for MAEA  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_001017405  NM_005882  

REFSEQ mRNAs for MAEA gene (2 alternative transcripts): 

NM_001017405.1   NM_005882.3   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_001017405  NM_005882  

               OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_001017405
                                 Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_001017405
                                 untagged cDNA clones in CMV expression vector (see all 2): NM_001017405 

Additional cDNA sequence: 

AB208907.1 AF084928.1 AK001088.1 AK022515.1 AK022586.1 AK091951.1 AK094253.1 AK127875.1 
AK296174.1 AK298944.1 AK300572.1 AK301155.1 AK308901.1 AY189687.1 AY236486.1 BC001225.1 
BC006470.1 BT006957.1 CR601639.1 CR606463.1 CR615926.1 

24/34 DOTS entries (see all 34 ):

DT.102828944  DT.100803262  DT.95363778  DT.102828943  DT.100823297  DT.91944969  DT.101981808  DT.99994246 
DT.445825  DT.121266255  DT.91758643  DT.100823303  DT.121266257  DT.121266296  DT.100823305  DT.91982110 
DT.100823310  DT.121266305  DT.97825508  DT.100823300  DT.121266128  DT.121266139  DT.100823295  DT.100823296 

24/468 AceView cDNA sequences (see all 468 ):

AL599051 BG674252 BQ643464 AY189687 AK091951 BU622852 CA407470 AA890207 
AI468871 AI078734 BX380184 BG116905 BM752947 BU846328 AA291394 BQ942851 
BC001225 CA440896 AI421773 BU931849 AL521008 BM763749 BE550620 CA444845 

highest scoring ESTs for MAEA:

AA025521 AA041297 AA069148 AA079028 AA149893 AA150393 AA159350 AA215452 AA224400 AA291394 

Unigene Cluster for MAEA:

Macrophage erythroblast attacher
Hs.139896  [show with all ESTs]
Unigene Representative Sequence: AB208907


GeneLoc Exon Structure

5/6 Alternative Splicing Database (ASD) splice patterns (SP) for MAEA (see all 6 )

ExUns: 1 ^ 2 ^ 3 ^ 4a · 4b · 4c · 4d ^ 5a · 5b ^ 6 ^ 7a · 7b ^ 8 ^ 9a · 9b ^ 10
SP1:        -                       -     -     -                                                   
SP2:                                                                                                
SP3:        -           -     -     -     -                                                         
SP4:                                                                                                
SP5:                                                                                                

About this scheme

ECgene alternative splicing isoforms for MAEA

3 Ensembl transcripts including schematic representations:
ENST00000303400  ENST00000264750  ENST00000382947  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

MAEA expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for MAEA

1 / 2 / 3

3 probe-sets matching MAEA gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

32832_at2, 3 U95-A 1 1.00 1.00 1.00 1.00 AF084928 0.80 0.88 0.84 1

207922_s_at2, 3 U133-A 1 1.00 1.00 -- -- NM_005882 0.60 1.00 0.82 1

207922_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
About this table
Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: CGAATAAAAT

SOURCE GeneReport for Unigene cluster: Hs.139896

Expression variation in blood from EXPOLDB for MAEA

UniProtKB/Swiss-Prot: MAEA_HUMAN, Q7L5Y9
Tissue specificity: Ubiquitous

(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for MAEA gene from 5/20 species (see all 20 )
Organism Gene Locus Description Human
Similarity
NCBI accessions
dog
(Canis familiaris)
MAEA1   -- macrophage erythroblast attacher 92.14(n)
99.2(a)
610745  XM_848291.1  XP_853384.1 
chimpanzee
(Pan troglodytes)
MAEA1   -- macrophage erythroblast attacher 99.33(n)
99.75(a)
461061  XM_517061.2  XP_517061.2 
cow
(Bos taurus)
MAEA1   -- macrophage erythroblast attacher 91.58(n)
99.24(a)
511956  NM_001034415.1  NP_001029587.1 
rat
(Rattus norvegicus)
Maea1   -- macrophage erythroblast attacher 89.87(n)
98.7(a)
298982  NM_001008319.1  NP_001008320.1 
mouse
(Mus musculus)
Maea1, 5 55
macrophage erythroblast attacher1, 5 89.39(n)1
98.74(a)1
590031  NM_021500.21  NP_067475.21 
 AA0345455  AF2632475  (see all 26)
About this table        Species with no ortholog for MAEA

ENSEMBL Gene Tree for MAEA
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

Paralogs for MAEA gene
RMND5B2  RMND5A2  

(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section


10/475 NCBI SNPs in MAEA are shown (see all 475 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 146)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 4 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
--
rs9226981,2
A,C,F,H,O1272722(-) AAAGTA/GTTATT 2 -- ng518Minor allele frequency- G:0.43MN EU EA WA NA 744
rs112479831,2
A,C,F,H1271951(+) gatgcC/Gtacat 2 -- ng51 tfbs34Minor allele frequency- G:0.19EU EA WA 420
--
rs96318041,2
A,C,F,H1273032(+) GGCTGT/AAATGA 2 -- ng516Minor allele frequency- A:0.49EU EA WA MN 768
rs17321001,2
A,F,H1271761(-) CCAACA/GTTCTT 2 -- ng514Minor allele frequency- G:0.46EU EA WA 418
--
rs17320991,2
A,C,F1272696(-) TGCAGG/ACTCCA 2 -- ng513Minor allele frequency- A:0.47NA 136
rs76798601,2
A,C1323987(+) CTCCCC/TGGGGC 2 -- ng310--------
--
rs96318051,2
F1273035(+) TGAAAT/CGAAGG 2 -- ng512Minor allele frequency- C:0.46MN EA 356
--
rs171645871,2
F1273535(+) ACCCGG/ACAGTG 2 -- ng514Minor allele frequency- A:0.01MN EA 712
--
rs11285971,2
F1323801(+) GAACCA/CCCGTA 2 -- ut31 ese31Minor allele frequency- C:0.03MN 184
--
rs171645891,2
F1273608(+) GGCCCG/TCCCCC 2 -- ng512Minor allele frequency- T:0.20MN EA 356
About this table

HapMap Linkage Disequilibrium images for MAEA (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 606801

(Possibly Related Articles in Doctor's Guide)
About This Section

  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
About This Section

9 PubMed articles for MAEA gene:
(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 10296 HGNC: 13731 AceView: MAEA Ensembl:ENSG00000090316 euGenes: HUgn10296
ECgene: MAEA H-InvDB: MAEA
(According to HUGE)
About This Section

  --
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

  --
(Available from WIS Yeda, Salk, Tufts)
About This Section

  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for MAEA:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



  Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
  Invitrogen Antibodies  Invitrogen Assays
  Invitrogen Clones  Invitrogen Q-PCR Products
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  Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for MAEA-related products

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 Millipore Preclinical / Clinical Development Services
 Millipore Immunoassay Services
 Millipore Target Screening & Profiling Services


 Predesigned and custom siRNAs for MAEA Browse antibodies at Sigma-Aldrich
 Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
 Lentivirus-delivered shRNAs for MAEA Browse small molecules at Sigma-Aldrich
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 Tagged/untagged cDNA clones
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 shRNA in GFP-retroviral vector
 Search Tocris compounds for MAEA




 Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
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