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NBR1 Gene

protein-coding   GIFtS: 54

GC17P038578
neighbor of BRCA1 gene 1
(Previous names: membrane component, chromosome 17, surface marker 2 (ovarian carcinoma antigen CA125) )
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
(Previous symbol: M17S2)
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases
1A1-3B 1, 2, 3
1A13B 3
CA125 1
FLJ55359 2
FLJ98272 2
KIAA0049 1, 2, 3
M17S2 2, 3, 5
MIG19 2
Descriptions
Membrane component, chromosome 17, surface marker 2 3
Neighbor of BRCA1 gene 1 protein 3
membrane component, chromosome 17, surface marker 2
(ovarian carcinoma antigen CA125) 1, 2
migration-inducing protein 19 2
neighbor of BRCA1 gene 1 2
External Ids
HGNC: 67461
Entrez Gene: 40772
UniProtKB: Q145963
Search outside databases for aliases for NBR1 gene

Previous GC identifer: GC17P9L0001

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for NBR1:
The protein encoded by this gene was originally identified as an ovarian tumor antigen monitored in
ovarian cancer. The encoded protein contains a B-box/coiled coil motif, which is present in many
genes with transformation potential, but the function of this protein is unknown. This gene is
located on a region of chromosome 17q21.1 that is in close proximity to tumor suppressor gene
BRCA1. Three alternatively spliced variants encoding the same protein have been identified for
this gene. [provided by RefSeq]

Gene Wiki entry for NBR1
(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the NBR1 gene  

Entrez Gene cytogenetic band: 17q21.31   HGNC cytogenetic band: 17q21.31

NBR1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 17         GeneLoc Exon Structure

GeneLoc location for GC17P038578:     (about GC identifiers)

Start:
38,576,024 bp from pter
End:
38,600,582 bp from pter
Size:
24,559 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000017.9  NT_010755.15  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: NBR1_HUMAN, Q14596 (See protein sequence)
Recommended Name: Next to BRCA1 gene 1 protein  
Size: 966 amino acids; 107432 Da
Subunit: Homooligomer and heterooligomer. Interacts with SQSTM1, titin/TTN and RNF29
Caution: Was originally (PubMed:8069304) thought to be the ovarian carcinoma antigen CA125
PDB structures from and Proteopedia :
1WJ6 (3D)    2BKF (3D)    2CP8 (3D)    2G4S (3D)    
Secondary accessions: Q13173 Q15026 Q96GB6 Q9NRF7
Alternative splicing: 2 isoforms:  Q14596-1   Q14596-2   

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins (3 alternative transcripts): 
    NP_005890.2  NP_114064.1  NP_114068.1  

    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Enzo Life Sciences proteins for NBR1
    Recombinant Proteins from Abcam (NBR1)
    Human Recombinant Proteins from Abnova (NBR1)
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 


    Antibodies for NBR1: 
    Browse Antibodies Central at Invitrogen
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    Antibodies from Abcam (NBR1), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (NBR1)
    Novus Biologicals Antibodies for NBR1

    Assays for NBR1: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    4 InterPro domains/families:
     IPR000270 OPR_PB1
     IPR000449 UBA/transl_elong_EF1B_N
     IPR015940 UBA/transl_elong_EF1B_N_euk
     IPR000433 Znf_ZZ


       GeneDecks  NBR1 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q14596

    ProtoNet protein and cluster: Q14596

    3 Blocks protein families:
    IPB000270 Octicosapeptide/Phox/Bem1p
    IPB000433 Zn-finger
    IPB000449 Ubiquitin-associated domain


    UniProtKB/Swiss-Prot: NBR1_HUMAN, Q14596
    Domain: The OPR domain mediates interaction with SQSTM1
    Similarity: Contains 1 OPR domain
    Similarity: Contains 1 UBA domain
    Similarity: Contains 1 ZZ-type zinc finger

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (NBR1)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (NBR1)
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 4): NM_005899

                  Applied Biosystems Silencer® siRNAs for NBR1

                  Sigma-Aldrich siRNA and siRNA Panels for NBR1  
                         Sigma-Aldrich shRNA Panels and shRNA for NBR1  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for NBR1
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                   OriGene GFP tagged cDNA clones in CMV expression vector (see all 3): NM_005899
                                     Myc/DDK tagged cDNA clones in CMV expression vector (see all 3): NM_005899
                                     untagged cDNA clones in CMV expression vector (see all 4): BC009808 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_005899

    2 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0008270 zinc ion binding IEA--
    GO:0046872 metal ion binding IEA--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


     Gene Network CentralTM Interacting Genes and Proteins Network for  NBR1 


    5/15 Interacting proteins for NBR1 (Q145962) via UniProtKB, MINT, and/or STRING (see all 15 )
    InteractantInteraction Details
    GeneCardExternal ID(s)
    MAP1LC3AQ9H4922MINT-7034375 MINT-7034418 MINT-7034400
    USP8P408182MINT-7034470 MINT-7034233 MINT-7034452
    RPS27AP629882MINT-7034350 MINT-7034309
    RPS27AP11P629882MINT-7034350 MINT-7034309
    RPS27AP12P629882MINT-7034350 MINT-7034309
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for NBR1

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (NBR1)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (NBR1)
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 4): NM_005899

                  Sigma-Aldrich siRNA and siRNA Panels for NBR1  
                         Sigma-Aldrich shRNA Panels and shRNA for NBR1  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_005899  NM_031858  NM_031862  

    REFSEQ mRNAs for NBR1 gene (3 alternative transcripts): 

    NM_005899.3   NM_031858.2   NM_031862.2   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_005899  NM_031858  NM_031862  

                   OriGene GFP tagged cDNA clones in CMV expression vector (see all 3): NM_005899
                                     Myc/DDK tagged cDNA clones in CMV expression vector (see all 3): NM_005899
                                     untagged cDNA clones in CMV expression vector (see all 4): BC009808 

    Additional cDNA sequence: 

    AK308324.1 AY450308.1 D30756.1 X76952.1 XM_001124828.2 

    24/30 DOTS entries (see all 30 ):

    DT.102839957  DT.100822404  DT.100822409  DT.101978440  DT.100022668  DT.100822401  DT.444984  DT.120910716 
    DT.97858444  DT.120910787  DT.75190497  DT.40108675  DT.100816331  DT.70103187  DT.75119216  DT.87015353 
    DT.91855250  DT.91924762  DT.87008244  DT.95263448  DT.437274  DT.92464067  DT.95305503  DT.100728131 

    24/77 AceView cDNA sequences (see all 77 ):

    AU140463 AL044169 CB989852 CB988916 BF724610 AF227189 BM459691 CF138277 
    AW025470 CF135984 AL042510 AW236032 AL039232 BQ924406 M79023 CB160484 
    AU140448 BU161042 BM827388 BM925429 BX452264 BU152844 BU173368 BX462113 

    highest scoring ESTs for NBR1:

    D30756 AA028089 AA028090 AA046065 AA046348 AA069906 AA083814 AA126012 AA126134 AA126858 

    Unigene Clusters for NBR1:

    Neighbor of BRCA1 gene 1
    Hs.373818  [show with all ESTs], Hs.546264  [show with all ESTs]
    Unigene Representative Sequences: AK290005, NM_031858


    GeneLoc Exon Structure

    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    NBR1 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for NBR1

    1 / 2 / 3

    5 probe-sets matching NBR1 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
    33444_at2, 3 U95-A 3 1.00 0.33 1.00 1.00 D30756 1.00 0.90 0.95 1

    201384_s_at2, 3 U133-A 3 1.00 0.39 -- -- NM_005899 0.60 1.00 0.82 1

    201383_s_at2, 3 U133-A 3 1.00 0.33 -- -- AL044170 0.60 1.00 0.82 1

    201384_s_at2 U133Plus2 3 1.00 0.39 -- -- -- -- -- -- --

    201383_s_at2 U133Plus2 3 1.00 0.33 -- -- -- -- -- -- --
    GeneDecks  NBR1 for binary patterns associated with the probe-sets selected above  
    About GeneDecksing
    About this table    
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: --

    SOURCE GeneReport for Unigene clusters: Hs.373818 Hs.546264

    Expression variation in blood from EXPOLDB for NBR1

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for NBR1 gene from 5/9 species (see all 9 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    NBR11   -- neighbor of BRCA1 gene 1 89.53(n)
    88.55(a)
    480509  XM_537628.2  XP_537628.2 
    chimpanzee
    (Pan troglodytes)
    NBR11   -- neighbor of BRCA1 gene 1 99.62(n)
    99.38(a)
    455047  XM_001155005.1  XP_001155005.1 
    cow
    (Bos taurus)
    NBR11   -- neighbor of BRCA1 gene 1 90.1(n)
    88.08(a)
    515032  XM_592972.3  XP_592972.2 
    rat
    (Rattus norvegicus)
    Nbr11   -- neighbor of Brca1 gene 1 87.36(n)
    85.65(a)
    303554  NM_001024765.1  NP_001019936.1 
    mouse
    (Mus musculus)
    Nbr11, 5 11 (60.50 cM)5
    neighbor of Brca1 gene 11, 5 87.01(n)1
    85.89(a)1
    179661  NM_008676.21  NP_032702.11 
     AF2271885  AF4084365  (see all 22)
    About this table        Species with no ortholog for NBR1
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

      --
    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/109 NCBI SNPs in NBR1 are shown (see all 109 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 24)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 17 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    --
    rs20113161,2
    H38574089(-) ttctcC/Atgcct 2 -- ng514Minor allele frequency- A:0.00EU EA WA 418
    rs360543171,2
    F38595151(+) GTTAAC/TGAAAC 3 N/N syn11Minor allele frequency- T:0.04NA 76
    rs72200091,2
    A,C,F,H38584238(+) AAATTA/GTATAG 3 -- int17Minor allele frequency- G:0.03EU EA WA NA 554
    rs116532311,2
    A,C,F,H38584986(+) GCCTTG/ATTTAA 3 -- int16Minor allele frequency- A:0.27EU EA WA NA 592
    --
    rs13993231,2
    A,C,F,H38585856(-) CCTATA/GAGCAA 3 -- int17Minor allele frequency- G:0.29NA EU EA WA 556
    rs116534601,2
    A,C,F,H38584832(+) CTGAAC/TGCTTG 3 -- int14Minor allele frequency- T:0.25EU EA WA 418
    rs42391491,2
    A,C,F,H38581621(+) CCCCAG/ATAAAG 3 -- int18Minor allele frequency- A:0.50EU EA WA NA 634
    rs116516231,2
    A,C,F,H38583373(+) GTTATG/AGGTTA 3 -- int14Minor allele frequency- A:0.25EU EA WA 412
    rs116509131,2
    A,C,F,H38585123(+) ATATAT/CAGGAT 3 -- int14Minor allele frequency- C:0.25EU EA WA 408
    rs80700851,2
    A,C,F,H38595510(+) TTTGTA/GTGTTG 3 -- int111Minor allele frequency- G:0.29EU EA WA NA 972
    About this table

    HapMap Linkage Disequilibrium images for NBR1 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 166945

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/18 PubMed articles for NBR1 gene (see all 18 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 4077 HGNC: 6746 AceView: LOC439923 euGenes: HUgn4077 ECgene: NBR1
    H-InvDB: NBR1
    (According to HUGE)
    About This Section

    HUGE: KIAA0049
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

    NameDescription
    ATLAS Chromosomes in Cancer entry for NBR1 Genetics and Cytogenetics in Oncology and Haematology
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for NBR1:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



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     Predesigned and custom siRNAs for NBR1 Antibodies for NBR1
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for NBR1 Browse small molecules at Sigma-Aldrich
     "Your Favorite Gene" Pathwaysfeedback


      
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     Recombinant Proteins
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     Proteins for NBR1
     Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
     Antibodies for NBR1

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