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Aliases for LRWD1 Gene

Aliases for LRWD1 Gene

  • Leucine-Rich Repeats And WD Repeat Domain Containing 1 2 3
  • Centromere Protein 33 2 3 4
  • ORCA 3 4 6
  • Origin Recognition Complex-Associated Protein 3 4
  • Origin Recognition Complex Associated 2 3
  • ORC-Associated Protein 3 4
  • CENP-33 3 4
  • Leucine-Rich Repeat And WD Repeat-Containing Protein 1 3
  • CENP33 4

External Ids for LRWD1 Gene

Summaries for LRWD1 Gene

GeneCards Summary for LRWD1 Gene

LRWD1 (Leucine-Rich Repeats And WD Repeat Domain Containing 1) is a Protein Coding gene. GO annotations related to this gene include chromatin binding and methyl-CpG binding.

UniProtKB/Swiss-Prot for LRWD1 Gene

  • Required for G1/S transition. Recruits and stabilizes the origin recognition complex (ORC) onto chromatin during G1 to establish pre-replication complex (preRC) and to heterochromatic sites in post-replicated cells. Binds a combination of DNA and histone methylation repressive marks on heterochromatin. Binds histone H3 and H4 trimethylation marks H3K9me3, H3K20me3 and H4K27me3 in a cooperative manner with DNA methylation. Required for silencing of major satellite repeats. May be important ORC2, ORC3 and ORC4 stability.

No data available for Entrez Gene Summary , Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for LRWD1 Gene

Genomics for LRWD1 Gene

Genomic Location for LRWD1 Gene

Start:
102,464,888 bp from pter
End:
102,473,168 bp from pter
Size:
8,281 bases
Orientation:
Plus strand

Genomic View for LRWD1 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for LRWD1 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for LRWD1 Gene

Regulatory Elements for LRWD1 Gene

Proteins for LRWD1 Gene

  • Protein details for LRWD1 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q9UFC0-LRWD1_HUMAN
    Recommended name:
    Leucine-rich repeat and WD repeat-containing protein 1
    Protein Accession:
    Q9UFC0
    Secondary Accessions:
    • A8K4K2
    • B2R9G2
    • Q8N0T9
    • Q8WV43
    • Q96GJ2

    Protein attributes for LRWD1 Gene

    Size:
    647 amino acids
    Molecular mass:
    70861 Da
    Quaternary structure:
    • Integral component of the ORC complex. Directly interacts with CDT1, GMNN and ORC2. Interacts with ORC2 only when non-ubiquitinated; this interaction prevents LRWD1 ubiquitination and degradation. Some of these interactions are regulated in a cell-cycle dependent manner. Interaction with ORC1 occurs predominantly during G1. Association with phosphorylated ORC1 during mitosis is not efficient. Interaction with CDT1 occurs during G1 phase, as well as during mitosis with phosphorylated CDT1. Interaction with GMNN occurs from G1/S to mitosis. Interaction with ORC2 is observed throughout the cell cycle. The stoichiometry of the ORCA/ORC/CDT1/GMNN complex is 1:1:1:2. Interacts with CUL4A and DDB1; this interaction may lead to ubiquitination.

neXtProt entry for LRWD1 Gene

Proteomics data for LRWD1 Gene at MOPED

Post-translational modifications for LRWD1 Gene

  • Ubiquitinated; undergoes Lys-48-linked polyubiquitination leading to proteasomal degradation. Ubiquitination occurs within the WD repeats at the end of the G1 phase. Ubiquitination may be catalyzed by the CUL4-DDB1 E3 ubiquitin-protein ligase complex and other E3 ligases.
  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Other Protein References for LRWD1 Gene

No data available for DME Specific Peptides for LRWD1 Gene

Domains for LRWD1 Gene

Gene Families for LRWD1 Gene

HGNC:
  • WDR :WD repeat domain containing

Suggested Antigen Peptide Sequences for LRWD1 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

Q9UFC0

UniProtKB/Swiss-Prot:

LRWD1_HUMAN
Domain:
  • The entire WD repeat region is required for the interaction with ORC, CDT1 and GMNN, as well as for association with chromatin and for binding to histone H3 and H4 trimethylation marks H3K9me3 and H4K20me3.:
    • Q9UFC0
Family:
  • Belongs to the LRWD1 family.:
    • Q9UFC0
Similarity:
  • Contains 4 LRR (leucine-rich) repeats.:
    • Q9UFC0
  • Contains 7 WD repeats.:
    • Q9UFC0
genes like me logo Genes that share domains with LRWD1: view

Function for LRWD1 Gene

Molecular function for LRWD1 Gene

UniProtKB/Swiss-Prot Function: Required for G1/S transition. Recruits and stabilizes the origin recognition complex (ORC) onto chromatin during G1 to establish pre-replication complex (preRC) and to heterochromatic sites in post-replicated cells. Binds a combination of DNA and histone methylation repressive marks on heterochromatin. Binds histone H3 and H4 trimethylation marks H3K9me3, H3K20me3 and H4K27me3 in a cooperative manner with DNA methylation. Required for silencing of major satellite repeats. May be important ORC2, ORC3 and ORC4 stability.

Gene Ontology (GO) - Molecular Function for LRWD1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003682 chromatin binding IDA 20932478
GO:0005515 protein binding IPI 20932478
GO:0008327 methyl-CpG binding IDA 21029866
GO:0035064 methylated histone binding IDA 21029866
genes like me logo Genes that share ontologies with LRWD1: view

miRNA for LRWD1 Gene

miRTarBase miRNAs that target LRWD1

No data available for Enzyme Numbers (IUBMB) , Phenotypes , Animal Models , Transcription Factor Targeting and HOMER Transcription for LRWD1 Gene

Localization for LRWD1 Gene

Subcellular locations from UniProtKB/Swiss-Prot for LRWD1 Gene

Nucleus. Chromosome, centromere. Chromosome, telomere. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Localizes to heterochromatin during G1 phase. Restricted to centromeres or telomeres as cells progress though S phase. When cells enter mitosis, relocalizes to centromeres. Recruitment to pericentric heterochromatin largely depends on the presence of H3K9me3.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for LRWD1 Gene COMPARTMENTS Subcellular localization image for LRWD1 gene
Compartment Confidence
nucleus 5
cytosol 2
mitochondrion 1
peroxisome 1

Gene Ontology (GO) - Cellular Components for LRWD1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IDA 21029866
GO:0005664 nuclear origin of replication recognition complex IDA 20932478
GO:0005721 pericentric heterochromatin IDA 20932478
GO:0005737 cytoplasm IEA --
GO:0005815 microtubule organizing center IEA --
genes like me logo Genes that share ontologies with LRWD1: view

Pathways for LRWD1 Gene

SuperPathways for LRWD1 Gene

No Data Available

Gene Ontology (GO) - Biological Process for LRWD1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006270 DNA replication initiation TAS 20932478
GO:0006325 chromatin organization IMP 20932478
GO:0016568 chromatin modification IEA --
GO:0071169 establishment of protein localization to chromatin IDA 20932478
genes like me logo Genes that share ontologies with LRWD1: view

No data available for Pathways by source for LRWD1 Gene

Transcripts for LRWD1 Gene

Unigene Clusters for LRWD1 Gene

Leucine-rich repeats and WD repeat domain containing 1:
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for LRWD1 Gene

ExUns: 1a · 1b · 1c · 1d · 1e ^ 2 ^ 3 ^ 4 ^ 5a · 5b ^ 6a · 6b ^ 7 ^ 8a · 8b ^ 9 ^ 10 ^ 11a · 11b ^ 12 ^ 13 ^ 14 ^ 15 ^ 16a · 16b · 16c
SP1: - - - - -
SP2: - -
SP3:
SP4: -
SP5:
SP6: - - - - -
SP7:
SP8: -

Relevant External Links for LRWD1 Gene

GeneLoc Exon Structure for
LRWD1
ECgene alternative splicing isoforms for
LRWD1

Expression for LRWD1 Gene

mRNA expression in normal human tissues for LRWD1 Gene

mRNA differential expression in normal tissues according to GTEx for LRWD1 Gene

This gene is overexpressed in Testis (16.1).

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, MOPED, and MaxQB for LRWD1 Gene

SOURCE GeneReport for Unigene cluster for LRWD1 Gene Hs.274135

mRNA Expression by UniProt/SwissProt for LRWD1 Gene

Q9UFC0-LRWD1_HUMAN
Tissue specificity: Testis-specific. Drastically down-regulated in testis from patients with Sertoli cell-only syndrome (SCOS).
genes like me logo Genes that share expressions with LRWD1: view

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery for LRWD1 Gene

Orthologs for LRWD1 Gene

This gene was present in the common ancestor of chordates.

Orthologs for LRWD1 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia LRWD1 35
  • 99.33 (n)
  • 99.69 (a)
LRWD1 36
  • 100 (a)
OneToOne
cow
(Bos Taurus)
Mammalia DKFZP434K1815 36
  • 80 (a)
OneToOne
LRWD1 35
  • 84.69 (n)
  • 80.31 (a)
dog
(Canis familiaris)
Mammalia LRWD1 35
  • 87.32 (n)
  • 88.89 (a)
LRWD1 36
  • 86 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Lrwd1 35
  • 80.59 (n)
  • 78.42 (a)
Lrwd1 16
Lrwd1 36
  • 78 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia LRWD1 36
  • 53 (a)
OneToOne
chicken
(Gallus gallus)
Aves LRWD1 35
  • 55.24 (n)
  • 49.69 (a)
LRWD1 36
  • 48 (a)
OneToOne
African clawed frog
(Xenopus laevis)
Amphibia Xl.31991 35
tropical clawed frog
(Silurana tropicalis)
Amphibia lrwd1 35
  • 51.53 (n)
  • 47.59 (a)
zebrafish
(Danio rerio)
Actinopterygii lrwd1 35
  • 49.61 (n)
  • 38.41 (a)
lrwd1 36
  • 35 (a)
OneToOne
sea squirt
(Ciona savignyi)
Ascidiacea -- 36
  • 34 (a)
OneToOne
Species with no ortholog for LRWD1:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • lizard (Anolis carolinensis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • platypus (Ornithorhynchus anatinus)
  • rainbow trout (Oncorhynchus mykiss)
  • rat (Rattus norvegicus)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for LRWD1 Gene

ENSEMBL:
Gene Tree for LRWD1 (if available)
TreeFam:
Gene Tree for LRWD1 (if available)

Paralogs for LRWD1 Gene

No data available for Paralogs for LRWD1 Gene

Variants for LRWD1 Gene

Sequence variations from dbSNP and Humsavar for LRWD1 Gene

SNP ID Clin Chr 07 pos Sequence Context AA Info Type MAF
rs3800987 -- 102,472,533(+) TCCAC(A/G)GTGGC synonymous-codon, reference, intron-variant
rs4727526 -- 102,467,906(+) GGGCA(C/T)GTGCC intron-variant
rs4729791 -- 102,467,705(+) ATGGC(C/T)TGGCC intron-variant
rs6959500 -- 102,472,203(+) CATCT(C/T)GTGCT intron-variant
rs6971245 -- 102,467,565(+) GAGGG(C/G)CTGTG intron-variant

Structural Variations from Database of Genomic Variants (DGV) for LRWD1 Gene

Variant ID Type Subtype PubMed ID
nsv428182 CNV Gain 18775914
nsv831083 CNV Loss 17160897
nsv464664 CNV Gain 19166990
nsv464665 CNV Loss 19166990
dgv7412n71 CNV Loss 21882294
nsv888832 CNV Loss 21882294
nsv5879 CNV Loss 18451855
nsv8192 CNV Gain 18304495
dgv7413n71 CNV Loss 21882294
esv27624 CNV Gain+Loss 19812545
nsv471450 CNV Duplication 19718026
nsv482111 CNV Gain 20164927

Relevant External Links for LRWD1 Gene

HapMap Linkage Disequilibrium report
LRWD1

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for LRWD1 Gene

Disorders for LRWD1 Gene

(1) University of Copenhagen DISEASES for LRWD1 Gene

genes like me logo Genes that share disorders with LRWD1: view

No data available for UniProtKB/Swiss-Prot for LRWD1 Gene

Publications for LRWD1 Gene

  1. Quantitative interaction proteomics and genome-wide profiling of epigenetic histone marks and their readers. (PMID: 20850016) Vermeulen M. … Mann M. (Cell 2010) 2 3 4
  2. A WD-repeat protein stabilizes ORC binding to chromatin. (PMID: 20932478) Shen Z. … Prasanth S.G. (Mol. Cell 2010) 2 3 4
  3. Nucleosome-interacting proteins regulated by DNA and histone methylation. (PMID: 21029866) Bartke T. … Kouzarides T. (Cell 2010) 3 4
  4. Expression of lrwd1 in mouse testis and its centrosomal localization. (PMID: 20180869) Teng Y.N. … Kuo T.Y. (Int. J. Androl. 2010) 2 3
  5. Leucine-rich repeat and WD repeat-containing protein 1 is recruited to pericentric heterochromatin by trimethylated lysine 9 of histone H3 and maintains heterochromatin silencing. (PMID: 22427655) Chan K.M. … Zhang Z. (J. Biol. Chem. 2012) 3 4

Products for LRWD1 Gene

Sources for LRWD1 Gene

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