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Aliases for LGMN Gene

Aliases for LGMN Gene

  • Legumain 2 3
  • PRSC1 3 4 6
  • Protease, Cysteine, 1 (Legumain) 2 3
  • Asparaginyl Endopeptidase 3 4
  • Protease, Cysteine 1 3 4
  • EC 3.4.22.34 4 63
  • Cysteine Protease 1 3
  • LGMN1 3
  • AEP 3

External Ids for LGMN Gene

Previous HGNC Symbols for LGMN Gene

  • PRSC1

Previous GeneCards Identifiers for LGMN Gene

  • GC14M090677
  • GC14M086987
  • GC14M091160
  • GC14M092239
  • GC14M093170
  • GC14M073353

Summaries for LGMN Gene

Entrez Gene Summary for LGMN Gene

  • This gene encodes a cysteine protease that has a strict specificity for hydrolysis of asparaginyl bonds. This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Enzyme activation is triggered by acidic pH and appears to be autocatalytic. Protein expression occurs after monocytes differentiate into dendritic cells. A fully mature, active enzyme is produced following lipopolysaccharide expression in mature dendritic cells. Overexpression of this gene may be associated with the majority of solid tumor types. This gene has a pseudogene on chromosome 13. Several alternatively spliced transcript variants have been described, but the biological validity of only two has been determined. These two variants encode the same isoform. [provided by RefSeq, Jul 2008]

GeneCards Summary for LGMN Gene

LGMN (Legumain) is a Protein Coding gene. Among its related pathways are Class I MHC mediated antigen processing and presentation and Lysosome. GO annotations related to this gene include peptidase activity and cysteine-type endopeptidase activity. An important paralog of this gene is PIGK.

UniProtKB/Swiss-Prot for LGMN Gene

  • Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. Required for normal lysosomal protein degradation in renal proximal tubules. Required for normal degradation of internalized EGFR. Plays a role in the regulation of cell proliferation via its role in EGFR degradation (By similarity). May be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system.

Gene Wiki entry for LGMN Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for LGMN Gene

Genomics for LGMN Gene

Regulatory Elements for LGMN Gene

Genomic Location for LGMN Gene

Start:
92,703,807 bp from pter
End:
92,748,702 bp from pter
Size:
44,896 bases
Orientation:
Minus strand

Genomic View for LGMN Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for LGMN Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for LGMN Gene

Proteins for LGMN Gene

  • Protein details for LGMN Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q99538-LGMN_HUMAN
    Recommended name:
    Legumain
    Protein Accession:
    Q99538
    Secondary Accessions:
    • O00123
    • Q86TV2
    • Q86TV3
    • Q9BTY1

    Protein attributes for LGMN Gene

    Size:
    433 amino acids
    Molecular mass:
    49411 Da
    Quaternary structure:
    • Homodimer before autocatalytic removal of the propeptide (By similarity). Monomer after autocatalytic processing. May interact with integrins.

    Three dimensional structures from OCA and Proteopedia for LGMN Gene

    Alternative splice isoforms for LGMN Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for LGMN Gene

Proteomics data for LGMN Gene at MOPED

Post-translational modifications for LGMN Gene

Antibody Products

  • R&D Systems Antibodies for LGMN (Legumain/Asparaginyl Endopeptidase)

Domains for LGMN Gene

Protein Domains for LGMN Gene

Graphical View of Domain Structure for InterPro Entry

Q99538

UniProtKB/Swiss-Prot:

LGMN_HUMAN :
  • Q99538
Domain:
  • In the zymogen form, the uncleaved propeptide blocks access to the active site.
Family:
  • Belongs to the peptidase C13 family.
genes like me logo Genes that share domains with LGMN: view

No data available for Gene Families for LGMN Gene

Function for LGMN Gene

Molecular function for LGMN Gene

UniProtKB/Swiss-Prot BiophysicochemicalProperties: pH dependence: Optimum pH is 5.5 for the free enzyme, and pH 6 in the presence of bound integrins. {ECO:0000269 PubMed:23776206};
UniProtKB/Swiss-Prot CatalyticActivity: Hydrolysis of proteins and small molecule substrates at -Asn- -Xaa- bonds.
UniProtKB/Swiss-Prot Function: Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. Required for normal lysosomal protein degradation in renal proximal tubules. Required for normal degradation of internalized EGFR. Plays a role in the regulation of cell proliferation via its role in EGFR degradation (By similarity). May be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system.

Enzyme Numbers (IUBMB) for LGMN Gene

Gene Ontology (GO) - Molecular Function for LGMN Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004197 cysteine-type endopeptidase activity ISS --
GO:0008233 peptidase activity IDA 136644
genes like me logo Genes that share ontologies with LGMN: view
genes like me logo Genes that share phenotypes with LGMN: view

Animal Models for LGMN Gene

MGI Knock Outs for LGMN:

Animal Model Products

miRNA for LGMN Gene

No data available for Transcription Factor Targeting and HOMER Transcription for LGMN Gene

Localization for LGMN Gene

Subcellular locations from UniProtKB/Swiss-Prot for LGMN Gene

Lysosome.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for LGMN Gene COMPARTMENTS Subcellular localization image for LGMN gene
Compartment Confidence
lysosome 4
vacuole 4
endosome 2
extracellular 2
endoplasmic reticulum 1
peroxisome 1

Gene Ontology (GO) - Cellular Components for LGMN Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005764 lysosome ISS --
GO:0005770 late endosome IEA --
GO:0043202 lysosomal lumen TAS --
GO:0045177 apical part of cell IEA --
GO:0070062 extracellular exosome IDA 23533145
genes like me logo Genes that share ontologies with LGMN: view

Pathways for LGMN Gene

genes like me logo Genes that share pathways with LGMN: view

Interacting Proteins for LGMN Gene

Gene Ontology (GO) - Biological Process for LGMN Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0002224 toll-like receptor signaling pathway TAS --
GO:0003014 renal system process ISS --
GO:0006508 proteolysis ISS --
GO:0008202 steroid metabolic process TAS --
GO:0010447 response to acidic pH IEA --
genes like me logo Genes that share ontologies with LGMN: view

Compounds for LGMN Gene

(6) Novoseek inferred chemical compound relationships for LGMN Gene

Compound -log(P) Hits PubMed IDs
clostripain 81.7 3
cysteine 74.4 37
asparagine 65.5 9
aspartate 20.8 2
histidine 5.33 1
genes like me logo Genes that share compounds with LGMN: view

Transcripts for LGMN Gene

Unigene Clusters for LGMN Gene

Legumain:
Representative Sequences:

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for LGMN

Primer Products

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for LGMN Gene

ExUns: 1a · 1b ^ 2 ^ 3 ^ 4 ^ 5a · 5b ^ 6 ^ 7 ^ 8a · 8b ^ 9 ^ 10 ^ 11 ^ 12a · 12b ^ 13a · 13b ^ 14a · 14b · 14c · 14d · 14e
SP1: - - -
SP2:
SP3: - - -
SP4: - - - -
SP5:
SP6: - - -
SP7: - - -
SP8:

Relevant External Links for LGMN Gene

GeneLoc Exon Structure for
LGMN
ECgene alternative splicing isoforms for
LGMN

Expression for LGMN Gene

mRNA expression in normal human tissues for LGMN Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, MOPED, and MaxQB for LGMN Gene

SOURCE GeneReport for Unigene cluster for LGMN Gene Hs.18069

mRNA Expression by UniProt/SwissProt for LGMN Gene

Q99538-LGMN_HUMAN
Tissue specificity: Ubiquitous. Particularly abundant in kidney, heart and placenta
genes like me logo Genes that share expressions with LGMN: view

In Situ Assay Products

No data available for mRNA differential expression in normal tissues for LGMN Gene

Orthologs for LGMN Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for LGMN Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia LGMN 35
  • 99.15 (n)
  • 99.31 (a)
LGMN 36
  • 99 (a)
OneToOne
cow
(Bos Taurus)
Mammalia LGMN 35
  • 84.91 (n)
  • 82.91 (a)
LGMN 36
  • 83 (a)
OneToOne
dog
(Canis familiaris)
Mammalia LGMN 35
  • 83.22 (n)
  • 83.37 (a)
LGMN 36
  • 83 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Lgmn 35
  • 81.68 (n)
  • 83.37 (a)
Lgmn 16
Lgmn 36
  • 83 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia LGMN 36
  • 73 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia LGMN 36
  • 67 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Lgmn 35
  • 82.29 (n)
  • 83.37 (a)
chicken
(Gallus gallus)
Aves LGMN 35
  • 73.78 (n)
  • 72.85 (a)
LGMN 36
  • 72 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia LGMN 36
  • 67 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia lgmn 35
  • 67.44 (n)
  • 68.53 (a)
rainbow trout
(Oncorhynchus mykiss)
Actinopterygii Omy.14073 35
zebrafish
(Danio rerio)
Actinopterygii lgmn 35
  • 67.53 (n)
  • 66.67 (a)
lgmn 36
  • 64 (a)
OneToOne
sbcb173 35
worm
(Caenorhabditis elegans)
Secernentea T28H10.3 35
  • 51.66 (n)
  • 43.28 (a)
T28H10.3 36
  • 37 (a)
OneToOne
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes GPI8 36
  • 22 (a)
OneToMany
Alicante grape
(Vitis vinifera)
eudicotyledons Vvi.7157 35
thale cress
(Arabidopsis thaliana)
eudicotyledons DELTA-VPE 35
  • 50.29 (n)
  • 42.26 (a)
rice
(Oryza sativa)
Liliopsida Os.4189 35
wheat
(Triticum aestivum)
Liliopsida Ta.9998 35
sea squirt
(Ciona intestinalis)
Ascidiacea Cin.5118 35
sea squirt
(Ciona savignyi)
Ascidiacea -- 36
  • 58 (a)
OneToOne
Species with no ortholog for LGMN:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)

Evolution for LGMN Gene

ENSEMBL:
Gene Tree for LGMN (if available)
TreeFam:
Gene Tree for LGMN (if available)

Paralogs for LGMN Gene

Paralogs for LGMN Gene

Pseudogenes.org Pseudogenes for LGMN Gene

genes like me logo Genes that share paralogs with LGMN: view

Variants for LGMN Gene

Sequence variations from dbSNP and Humsavar for LGMN Gene

SNP ID Clin Chr 14 pos Sequence Context AA Info Type MAF
rs9791 -- 92,704,648(-) TATCC(A/G)CTTCA synonymous-codon, reference, intron-variant
rs10465 -- 92,704,032(-) AGGAG(A/C)AGGAA utr-variant-3-prime
rs716097 -- 92,712,122(+) GATTA(C/T)AATAA intron-variant
rs1010651 -- 92,725,126(-) AGCAC(A/C)GGACC intron-variant
rs1015033 -- 92,728,841(-) ggtgg(A/G)gctaa intron-variant

Structural Variations from Database of Genomic Variants (DGV) for LGMN Gene

Variant ID Type Subtype PubMed ID
nsv1399 CNV Insertion 18451855
nsv456399 CNV Loss 19166990
nsv456400 CNV Loss 19166990
nsv521526 CNV Loss 19592680

Relevant External Links for LGMN Gene

HapMap Linkage Disequilibrium report
LGMN

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for LGMN Gene

Disorders for LGMN Gene

(10) Novoseek inferred disease relationships for LGMN Gene

Disease -log(P) Hits PubMed IDs
schistosomiasis 34 1
tetanus 27.3 1
solid tumor 24.4 4
multiple sclerosis 7.75 1
metastasis 7.38 4
genes like me logo Genes that share disorders with LGMN: view

No data available for UniProtKB/Swiss-Prot for LGMN Gene

Publications for LGMN Gene

  1. Cloning, isolation, and characterization of mammalian legumain, an asparaginyl endopeptidase. (PMID: 9065484) Chen J.-M. … Barrett A.J. (J. Biol. Chem. 1997) 2 3 4 23
  2. Autocatalytic activation of human legumain at aspartic acid residues. (PMID: 9821970) Halfon S. … Zurawski G. (FEBS Lett. 1998) 3 4 23
  3. Activation of human prolegumain by cleavage at a C-terminal asparagine residue. (PMID: 11085925) Chen J.-M. … Barrett A.J. (Biochem. J. 2000) 3 4 23
  4. Molecular cloning of a human cDNA encoding putative cysteine protease (PRSC1) and its chromosome assignment to 14q32.1. (PMID: 8893817) Tanaka T. … Nakamura Y. (Cytogenet. Cell Genet. 1996) 2 3 4
  5. Cystatin E/M suppresses legumain activity and invasion of human melanoma. (PMID: 20074384) Briggs J.J. … Solberg R. (BMC Cancer 2010) 3 23

Products for LGMN Gene

Sources for LGMN Gene

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