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Aliases for LEF1 Gene

Aliases for LEF1 Gene

  • Lymphoid Enhancer-Binding Factor 1 2 3
  • T Cell-Specific Transcription Factor 1-Alpha 3 4
  • TCF1-Alpha 3 4
  • LEF-1 3 4
  • TCF1ALPHA 3
  • TCF7L3 3
  • TCF10 3

External Ids for LEF1 Gene

Previous GeneCards Identifiers for LEF1 Gene

  • GC04M109394
  • GC04M109289
  • GC04M109427
  • GC04M109326
  • GC04M109188
  • GC04M108968
  • GC04M104700

Summaries for LEF1 Gene

Entrez Gene Summary for LEF1 Gene

  • This gene encodes a transcription factor belonging to a family of proteins that share homology with the high mobility group protein-1. The protein encoded by this gene can bind to a functionally important site in the T-cell receptor-alpha enhancer, thereby conferring maximal enhancer activity. This transcription factor is involved in the Wnt signaling pathway, and it may function in hair cell differentiation and follicle morphogenesis. Mutations in this gene have been found in somatic sebaceous tumors. This gene has also been linked to other cancers, including androgen-independent prostate cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]

GeneCards Summary for LEF1 Gene

LEF1 (Lymphoid Enhancer-Binding Factor 1) is a Protein Coding gene. Diseases associated with LEF1 include skin pilomatrix carcinoma and odontoma. Among its related pathways are Signaling by GPCR and Pathways in cancer. GO annotations related to this gene include sequence-specific DNA binding transcription factor activity and chromatin binding. An important paralog of this gene is TCF7L2.

UniProtKB/Swiss-Prot for LEF1 Gene

  • Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells.

Gene Wiki entry for LEF1 Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for LEF1 Gene

Genomics for LEF1 Gene

Regulatory Elements for LEF1 Gene

Genomic Location for LEF1 Gene

Start:
108,047,545 bp from pter
End:
108,168,956 bp from pter
Size:
121,412 bases
Orientation:
Minus strand

Genomic View for LEF1 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for LEF1 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for LEF1 Gene

Proteins for LEF1 Gene

  • Protein details for LEF1 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q9UJU2-LEF1_HUMAN
    Recommended name:
    Lymphoid enhancer-binding factor 1
    Protein Accession:
    Q9UJU2
    Secondary Accessions:
    • B4DG38
    • B7Z8E2
    • E9PDK3
    • Q3ZCU4
    • Q9HAZ0

    Protein attributes for LEF1 Gene

    Size:
    399 amino acids
    Molecular mass:
    44201 Da
    Quaternary structure:
    • Binds the armadillo repeat of CTNNB1 and forms a stable complex. Interacts with EP300, TLE1 and PIASG (By similarity). Binds ALYREF/THOC4, MDFI and MDFIC. Interacts with NLK.

    Alternative splice isoforms for LEF1 Gene

neXtProt entry for LEF1 Gene

Proteomics data for LEF1 Gene at MOPED

Post-translational modifications for LEF1 Gene

  • Phosphorylated at Thr-155 and/or Ser-166 by NLK. Phosphorylation by NLK at these sites represses LEF1-mediated transcriptional activation of target genes of the canonical Wnt signaling pathway.
  • Ubiquitination at Lys301, Lys324, Lys347, Lys382, and Lys373
  • Modification sites at PhosphoSitePlus

No data available for DME Specific Peptides for LEF1 Gene

Domains for LEF1 Gene

Protein Domains for LEF1 Gene

Suggested Antigen Peptide Sequences for LEF1 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

Q9UJU2

UniProtKB/Swiss-Prot:

LEF1_HUMAN :
  • Q9UJU2
Domain:
  • Proline-rich and acidic regions are implicated in the activation functions of RNA polymerase II transcription factors
  • Contains 1 HMG box DNA-binding domain.
Family:
  • Belongs to the TCF/LEF family.
genes like me logo Genes that share domains with LEF1: view

No data available for Gene Families for LEF1 Gene

Function for LEF1 Gene

Molecular function for LEF1 Gene

GENATLAS Biochemistry: lymphoid enhancer-binding factor,complexing with TCF4 and beta catenin for activation of wingless responsive genes
UniProtKB/Swiss-Prot Function: Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells.

Gene Ontology (GO) - Molecular Function for LEF1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000977 RNA polymerase II regulatory region sequence-specific DNA binding IDA 23001182
GO:0000978 RNA polymerase II core promoter proximal region sequence-specific DNA binding IEA --
GO:0001077 RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription IEA --
GO:0001228 RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription IMP 23001182
GO:0003677 DNA binding ISS --
genes like me logo Genes that share ontologies with LEF1: view
genes like me logo Genes that share phenotypes with LEF1: view

Animal Models for LEF1 Gene

MGI Knock Outs for LEF1:

miRNA for LEF1 Gene

miRTarBase miRNAs that target LEF1

No data available for Enzyme Numbers (IUBMB) , Transcription Factor Targeting and HOMER Transcription for LEF1 Gene

Localization for LEF1 Gene

Subcellular locations from UniProtKB/Swiss-Prot for LEF1 Gene

Nucleus. Note=Found in nuclear bodies upon PIASG binding. {ECO:0000250}.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for LEF1 Gene COMPARTMENTS Subcellular localization image for LEF1 gene
Compartment Confidence
nucleus 4
cytoskeleton 2
cytosol 1
extracellular 1
plasma membrane 1

Gene Ontology (GO) - Cellular Components for LEF1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IDA 17063141
GO:0005654 nucleoplasm TAS --
GO:0005667 transcription factor complex IDA 11751639
GO:0005737 cytoplasm IDA 19402906
GO:0032993 protein-DNA complex IDA 10825188
genes like me logo Genes that share ontologies with LEF1: view

Pathways for LEF1 Gene

genes like me logo Genes that share pathways with LEF1: view

Gene Ontology (GO) - Biological Process for LEF1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000122 negative regulation of transcription from RNA polymerase II promoter IEA --
GO:0001569 patterning of blood vessels IBA --
GO:0001649 osteoblast differentiation IEP 20128911
GO:0001755 neural crest cell migration IBA --
GO:0001756 somitogenesis IBA --
genes like me logo Genes that share ontologies with LEF1: view

Compounds for LEF1 Gene

(6) Novoseek inferred chemical compound relationships for LEF1 Gene

Compound -log(P) Hits PubMed IDs
glycogen 42.7 2
tyrosine 0 1
matrigel 0 1
alanine 0 1
cisplatin 0 2
genes like me logo Genes that share compounds with LEF1: view

Transcripts for LEF1 Gene

Unigene Clusters for LEF1 Gene

Lymphoid enhancer-binding factor 1:
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for LEF1 Gene

ExUns: 1a · 1b ^ 2 ^ 3 ^ 4 ^ 5a · 5b ^ 6 ^ 7 ^ 8 ^ 9a · 9b · 9c ^ 10 ^ 11 ^ 12a · 12b ^ 13a · 13b ^ 14a · 14b ^ 15 ^ 16 ^ 17 ^ 18 ^ 19a ·
SP1: - - - - - - - - - -
SP2: - - - - - - - - - - - -
SP3: - - - - - - - - - - -
SP4: - - - - -
SP5: - - - - - - - -
SP6: - - - - -
SP7: - - -
SP8: - - - - - -
SP9: - - - - - - - - -
SP10: - - - - - - - - - - -
SP11:

ExUns: 19b · 19c · 19d · 19e · 19f · 19g
SP1:
SP2:
SP3:
SP4:
SP5:
SP6:
SP7:
SP8:
SP9:
SP10:
SP11:

Relevant External Links for LEF1 Gene

GeneLoc Exon Structure for
LEF1
ECgene alternative splicing isoforms for
LEF1

Expression for LEF1 Gene

mRNA expression in normal human tissues for LEF1 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for LEF1 Gene

This gene is overexpressed in Whole Blood (5.9).

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, MOPED, and MaxQB for LEF1 Gene

SOURCE GeneReport for Unigene cluster for LEF1 Gene Hs.743478

mRNA Expression by UniProt/SwissProt for LEF1 Gene

Q9UJU2-LEF1_HUMAN
Tissue specificity: Detected in thymus. Not detected in normal colon, but highly expressed in colon cancer biopsies and colon cancer cell lines. Expressed in several pancreatic tumors and weakly expressed in normal pancreatic tissue. Isoforms 1 and 5 are detected in several pancreatic cell lines.
genes like me logo Genes that share expressions with LEF1: view

Orthologs for LEF1 Gene

This gene was present in the common ancestor of animals.

Orthologs for LEF1 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia LEF1 35
  • 99.75 (n)
  • 99.75 (a)
LEF1 36
  • 100 (a)
OneToOne
cow
(Bos Taurus)
Mammalia LEF1 35
  • 95.24 (n)
  • 99 (a)
LEF1 36
  • 99 (a)
OneToOne
dog
(Canis familiaris)
Mammalia LEF1 35
  • 95.57 (n)
  • 98.5 (a)
LEF1 36
  • 99 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Lef1 35
  • 91.52 (n)
  • 97.73 (a)
Lef1 16
Lef1 36
  • 98 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia LEF1 36
  • 97 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia LEF1 36
  • 89 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Lef1 35
  • 90.18 (n)
  • 96.73 (a)
chicken
(Gallus gallus)
Aves LEF-1 36
  • 95 (a)
OneToOne
LEF1 35
  • 85.25 (n)
  • 94.96 (a)
lizard
(Anolis carolinensis)
Reptilia LEF1 36
  • 93 (a)
OneToOne
African clawed frog
(Xenopus laevis)
Amphibia LOC398238 35
tropical clawed frog
(Silurana tropicalis)
Amphibia lef1 35
  • 80.8 (n)
  • 88.86 (a)
Str.10421 35
zebrafish
(Danio rerio)
Actinopterygii lef1 35
  • 77.38 (n)
  • 85.99 (a)
lef1 36
  • 86 (a)
OneToOne
fruit fly
(Drosophila melanogaster)
Insecta pan 36
  • 12 (a)
OneToMany
pan 37
  • 39 (a)
worm
(Caenorhabditis elegans)
Secernentea pop-1 36
  • 24 (a)
OneToMany
sea squirt
(Ciona intestinalis)
Ascidiacea Cin.1608 35
sea squirt
(Ciona savignyi)
Ascidiacea CSA.11069 36
  • 31 (a)
OneToMany
Species with no ortholog for LEF1:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for LEF1 Gene

ENSEMBL:
Gene Tree for LEF1 (if available)
TreeFam:
Gene Tree for LEF1 (if available)

Paralogs for LEF1 Gene

Paralogs for LEF1 Gene

Selected SIMAP similar genes for LEF1 Gene using alignment to 5 proteins:

genes like me logo Genes that share paralogs with LEF1: view

Variants for LEF1 Gene

Sequence variations from dbSNP and Humsavar for LEF1 Gene

SNP ID Clin Chr 04 pos Sequence Context AA Info Type MAF
rs739728 -- 108,056,474(+) ATGCA(A/C)CTAGG intron-variant
rs744369 -- 108,105,079(+) TGGAA(A/G)CGATA intron-variant
rs744370 -- 108,105,011(+) AGACT(C/T)TAAAA intron-variant
rs749414 -- 108,106,757(-) GAAAA(A/C)TAATT intron-variant
rs898518 -- 108,095,668(+) TAAAA(A/C)GCATG intron-variant

Structural Variations from Database of Genomic Variants (DGV) for LEF1 Gene

Variant ID Type Subtype PubMed ID
esv275379 CNV Loss 21479260
nsv4458 CNV Loss 18451855
nsv830032 CNV Gain 17160897

Relevant External Links for LEF1 Gene

HapMap Linkage Disequilibrium report
LEF1

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for LEF1 Gene

Disorders for LEF1 Gene

MalaCards: The human disease database

MalaCards: The human disease database. (4) Diseases for LEF1 Gene including...

Search for LEF1 Gene in MalaCards »

(2) University of Copenhagen DISEASES for LEF1 Gene

(26) Novoseek inferred disease relationships for LEF1 Gene

Disease -log(P) Hits PubMed IDs
neutropenia, severe congenital 73.4 2
familial adenomatous polyposis 60.8 4
pilomatricoma 57 3
colon carcinoma 47.2 5
colon cancer 40.1 11

Relevant External Links for LEF1

Genetic Association Database (GAD)
LEF1
Human Genome Epidemiology (HuGE) Navigator
LEF1
genes like me logo Genes that share disorders with LEF1: view

No data available for UniProtKB/Swiss-Prot for LEF1 Gene

Publications for LEF1 Gene

  1. Gene for lymphoid enhancer-binding factor 1 (LEF1) mapped to human chromosome 4 (q23-q25) and mouse chromosome 3 near Egf. (PMID: 1783375) Milatovich A. … Francke U. (Genomics 1991) 2 3 23
  2. A thymus-specific member of the HMG protein family regulates the human T cell receptor C alpha enhancer. (PMID: 2010090) Waterman M.L. … Jones K.A. (Genes Dev. 1991) 3 4 23
  3. ALY, a context-dependent coactivator of LEF-1 and AML-1, is required for TCRalpha enhancer function. (PMID: 9119228) Bruhn L. … Grosschedl R. (Genes Dev. 1997) 3 4 23
  4. The human LEF-1 gene contains a promoter preferentially active in lymphocytes and encodes multiple isoforms derived from alternative splicing. (PMID: 10756202) Hovanes K. … Waterman M.L. (Nucleic Acids Res. 2000) 3 4 23
  5. Beta-catenin-sensitive isoforms of lymphoid enhancer factor-1 are selectively expressed in colon cancer. (PMID: 11326276) Hovanes K. … Waterman M.L. (Nat. Genet. 2001) 3 4 23

Products for LEF1 Gene

  • Addgene plasmids for LEF1

Sources for LEF1 Gene

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