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Aliases for LEF1 Gene

Aliases for LEF1 Gene

  • Lymphoid Enhancer-Binding Factor 1 2 3
  • T Cell-Specific Transcription Factor 1-Alpha 3 4
  • TCF1-Alpha 3 4
  • LEF-1 3 4
  • TCF1ALPHA 3
  • TCF7L3 3
  • TCF10 3

External Ids for LEF1 Gene

Previous GeneCards Identifiers for LEF1 Gene

  • GC04M109394
  • GC04M109289
  • GC04M109427
  • GC04M109326
  • GC04M109188
  • GC04M108968
  • GC04M104700

Summaries for LEF1 Gene

Entrez Gene Summary for LEF1 Gene

  • This gene encodes a transcription factor belonging to a family of proteins that share homology with the high mobility group protein-1. The protein encoded by this gene can bind to a functionally important site in the T-cell receptor-alpha enhancer, thereby conferring maximal enhancer activity. This transcription factor is involved in the Wnt signaling pathway, and it may function in hair cell differentiation and follicle morphogenesis. Mutations in this gene have been found in somatic sebaceous tumors. This gene has also been linked to other cancers, including androgen-independent prostate cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]

GeneCards Summary for LEF1 Gene

LEF1 (Lymphoid Enhancer-Binding Factor 1) is a Protein Coding gene. Diseases associated with LEF1 include skin pilomatrix carcinoma and severe congenital neutropenia. Among its related pathways are Signaling by GPCR and Pathways in cancer. GO annotations related to this gene include transcription factor activity, sequence-specific DNA binding and chromatin binding. An important paralog of this gene is TCF7L2.

UniProtKB/Swiss-Prot for LEF1 Gene

  • Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells.

Gene Wiki entry for LEF1 Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for LEF1 Gene

Genomics for LEF1 Gene

Regulatory Elements for LEF1 Gene

Epigenetics Products

  • DNA Methylation CpG Assay Predesigned for Pyrosequencing in human,mouse,rat

Genomic Location for LEF1 Gene

Chromosome:
4
Start:
108,047,545 bp from pter
End:
108,168,956 bp from pter
Size:
121,412 bases
Orientation:
Minus strand

Genomic View for LEF1 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for LEF1 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for LEF1 Gene

Proteins for LEF1 Gene

  • Protein details for LEF1 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q9UJU2-LEF1_HUMAN
    Recommended name:
    Lymphoid enhancer-binding factor 1
    Protein Accession:
    Q9UJU2
    Secondary Accessions:
    • B4DG38
    • B7Z8E2
    • E9PDK3
    • Q3ZCU4
    • Q9HAZ0

    Protein attributes for LEF1 Gene

    Size:
    399 amino acids
    Molecular mass:
    44201 Da
    Quaternary structure:
    • Binds the armadillo repeat of CTNNB1 and forms a stable complex. Interacts with EP300, TLE1 and PIASG (By similarity). Binds ALYREF/THOC4, MDFI and MDFIC. Interacts with NLK.

    Alternative splice isoforms for LEF1 Gene

neXtProt entry for LEF1 Gene

Proteomics data for LEF1 Gene at MOPED

Post-translational modifications for LEF1 Gene

  • Phosphorylated at Thr-155 and/or Ser-166 by NLK. Phosphorylation by NLK at these sites represses LEF1-mediated transcriptional activation of target genes of the canonical Wnt signaling pathway.
  • Ubiquitination at Lys 324, Lys 382, and Lys 373
  • Modification sites at PhosphoSitePlus

Antibody Products

  • Cell Signaling Technology (CST) Antibodies for LEF1 (LEF-1)

No data available for DME Specific Peptides for LEF1 Gene

Domains & Families for LEF1 Gene

Protein Domains for LEF1 Gene

Suggested Antigen Peptide Sequences for LEF1 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

Q9UJU2

UniProtKB/Swiss-Prot:

LEF1_HUMAN :
  • Proline-rich and acidic regions are implicated in the activation functions of RNA polymerase II transcription factors
  • Belongs to the TCF/LEF family.
Domain:
  • Proline-rich and acidic regions are implicated in the activation functions of RNA polymerase II transcription factors
  • Contains 1 HMG box DNA-binding domain.
Family:
  • Belongs to the TCF/LEF family.
genes like me logo Genes that share domains with LEF1: view

No data available for Gene Families for LEF1 Gene

Function for LEF1 Gene

Molecular function for LEF1 Gene

GENATLAS Biochemistry:
lymphoid enhancer-binding factor,complexing with TCF4 and beta catenin for activation of wingless responsive genes
UniProtKB/Swiss-Prot Function:
Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells.

Gene Ontology (GO) - Molecular Function for LEF1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000977 RNA polymerase II regulatory region sequence-specific DNA binding IDA 23001182
GO:0000978 RNA polymerase II core promoter proximal region sequence-specific DNA binding IEA --
GO:0001077 transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding IEA --
GO:0001228 transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding IMP 23001182
GO:0003677 DNA binding ISS --
genes like me logo Genes that share ontologies with LEF1: view
genes like me logo Genes that share phenotypes with LEF1: view

Animal Models for LEF1 Gene

MGI Knock Outs for LEF1:

Animal Model Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for LEF1

In Situ Assay Products

Flow Cytometry Products

No data available for Enzyme Numbers (IUBMB) , Transcription Factor Targets and HOMER Transcription for LEF1 Gene

Localization for LEF1 Gene

Subcellular locations from UniProtKB/Swiss-Prot for LEF1 Gene

Nucleus. Note=Found in nuclear bodies upon PIASG binding. {ECO:0000250}.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for LEF1 Gene COMPARTMENTS Subcellular localization image for LEF1 gene
Compartment Confidence
nucleus 5
plasma membrane 3
cytosol 2
cytoskeleton 1
extracellular 1

Gene Ontology (GO) - Cellular Components for LEF1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IDA 17063141
GO:0005654 nucleoplasm TAS --
GO:0005667 transcription factor complex IDA 11751639
GO:0005737 cytoplasm IDA 19402906
GO:0032993 protein-DNA complex IDA 10825188
genes like me logo Genes that share ontologies with LEF1: view

Pathways & Interactions for LEF1 Gene

genes like me logo Genes that share pathways with LEF1: view

PCR Array Products

SIGNOR curated interactions for LEF1 Gene

Activates:
Is activated by:
Is inactivated by:

Gene Ontology (GO) - Biological Process for LEF1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000122 negative regulation of transcription from RNA polymerase II promoter IEA --
GO:0001569 patterning of blood vessels IBA --
GO:0001649 osteoblast differentiation IEP 20128911
GO:0001755 neural crest cell migration IBA --
GO:0001756 somitogenesis IBA --
genes like me logo Genes that share ontologies with LEF1: view

Drugs & Compounds for LEF1 Gene

(4) Drugs for LEF1 Gene - From: NovoSeek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Cisplatin Approved Pharma Inhibits DNA synthesis,chemotherapy drug, Platinum, Potent pro-apoptotic anticancer agent; activates caspase-3 2437
alanine Nutra 97
serine Nutra 420
tyrosine Nutra 547

(2) Additional Compounds for LEF1 Gene - From: NovoSeek

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
Glycogen
  • Animal starch
  • Glycogen
  • Liver starch
  • Lyoglycogen
  • Phytoglycogen
9005-79-2
Matrigel
genes like me logo Genes that share compounds with LEF1: view

Transcripts for LEF1 Gene

Unigene Clusters for LEF1 Gene

Lymphoid enhancer-binding factor 1:
Representative Sequences:

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for LEF1

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for LEF1 Gene

ExUns: 1a · 1b ^ 2 ^ 3 ^ 4 ^ 5a · 5b ^ 6 ^ 7 ^ 8 ^ 9a · 9b · 9c ^ 10 ^ 11 ^ 12a · 12b ^ 13a · 13b ^ 14a · 14b ^ 15 ^ 16 ^ 17 ^ 18 ^ 19a ·
SP1: - - - - - - - - - -
SP2: - - - - - - - - - - - -
SP3: - - - - - - - - - - -
SP4: - - - - -
SP5: - - - - - - - -
SP6: - - - - -
SP7: - - -
SP8: - - - - - -
SP9: - - - - - - - - -
SP10: - - - - - - - - - - -
SP11:

ExUns: 19b · 19c · 19d · 19e · 19f · 19g
SP1:
SP2:
SP3:
SP4:
SP5:
SP6:
SP7:
SP8:
SP9:
SP10:
SP11:

Relevant External Links for LEF1 Gene

GeneLoc Exon Structure for
LEF1
ECgene alternative splicing isoforms for
LEF1

Expression for LEF1 Gene

mRNA expression in normal human tissues for LEF1 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for LEF1 Gene

This gene is overexpressed in Whole Blood (x5.9).

Protein differential expression in normal tissues from HIPED for LEF1 Gene

This gene is overexpressed in Peripheral blood mononuclear cells (41.0), CD4 Tcells (18.0), and CD8 Tcells (10.0).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MOPED, and MaxQB for LEF1 Gene



SOURCE GeneReport for Unigene cluster for LEF1 Gene Hs.743478

mRNA Expression by UniProt/SwissProt for LEF1 Gene

Q9UJU2-LEF1_HUMAN
Tissue specificity: Detected in thymus. Not detected in normal colon, but highly expressed in colon cancer biopsies and colon cancer cell lines. Expressed in several pancreatic tumors and weakly expressed in normal pancreatic tissue. Isoforms 1 and 5 are detected in several pancreatic cell lines.
genes like me logo Genes that share expression patterns with LEF1: view

Protein tissue co-expression partners for LEF1 Gene

Primer Products

In Situ Assay Products

Orthologs for LEF1 Gene

This gene was present in the common ancestor of animals.

Orthologs for LEF1 Gene

Organism Taxonomy Gene Similarity Type Details
cow
(Bos Taurus)
Mammalia LEF1 35
  • 95.24 (n)
  • 99 (a)
LEF1 36
  • 99 (a)
OneToOne
dog
(Canis familiaris)
Mammalia LEF1 35
  • 95.57 (n)
  • 98.5 (a)
LEF1 36
  • 99 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Lef1 35
  • 91.52 (n)
  • 97.73 (a)
Lef1 16
Lef1 36
  • 98 (a)
OneToOne
chimpanzee
(Pan troglodytes)
Mammalia LEF1 35
  • 99.75 (n)
  • 99.75 (a)
LEF1 36
  • 100 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Lef1 35
  • 90.18 (n)
  • 96.73 (a)
oppossum
(Monodelphis domestica)
Mammalia LEF1 36
  • 97 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia LEF1 36
  • 89 (a)
OneToOne
chicken
(Gallus gallus)
Aves LEF1 35
  • 85.25 (n)
  • 94.96 (a)
LEF-1 36
  • 95 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia LEF1 36
  • 93 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia lef1 35
  • 80.8 (n)
  • 88.86 (a)
Str.10421 35
African clawed frog
(Xenopus laevis)
Amphibia LOC398238 35
zebrafish
(Danio rerio)
Actinopterygii lef1 35
  • 77.38 (n)
  • 85.99 (a)
lef1 36
  • 86 (a)
OneToOne
fruit fly
(Drosophila melanogaster)
Insecta pan 37
  • 39 (a)
pan 36
  • 12 (a)
OneToMany
worm
(Caenorhabditis elegans)
Secernentea pop-1 36
  • 24 (a)
OneToMany
sea squirt
(Ciona intestinalis)
Ascidiacea Cin.1608 35
sea squirt
(Ciona savignyi)
Ascidiacea CSA.11069 36
  • 31 (a)
OneToMany
Species with no ortholog for LEF1:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for LEF1 Gene

ENSEMBL:
Gene Tree for LEF1 (if available)
TreeFam:
Gene Tree for LEF1 (if available)

Paralogs for LEF1 Gene

Paralogs for LEF1 Gene

(3) SIMAP similar genes for LEF1 Gene using alignment to 5 proteins:

genes like me logo Genes that share paralogs with LEF1: view

Variants for LEF1 Gene

Sequence variations from dbSNP and Humsavar for LEF1 Gene

SNP ID Clin Chr 04 pos Sequence Context AA Info Type MAF
VAR_035935 A colorectal cancer sample

Structural Variations from Database of Genomic Variants (DGV) for LEF1 Gene

Variant ID Type Subtype PubMed ID
esv275379 CNV Loss 21479260
nsv4458 CNV Loss 18451855
nsv830032 CNV Gain 17160897

Variation tolerance for LEF1 Gene

Residual Variation Intolerance Score: 66.13% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 2.26; 40.60% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for LEF1 Gene

HapMap Linkage Disequilibrium report
LEF1

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for LEF1 Gene

Disorders for LEF1 Gene

MalaCards: The human disease database

(4) MalaCards diseases for LEF1 Gene - From: DISEASES, NovoSeek, and GeneCards

Disorder Aliases PubMed IDs
skin pilomatrix carcinoma
  • pilomatrix carcinoma of skin
severe congenital neutropenia
  • kostmann disease
colorectal cancer
  • colorectal cancer, somatic
prostate cancer
  • prostate cancer, hereditary, 5
- elite association
Search LEF1 in MalaCards View complete list of genes associated with diseases

Relevant External Links for LEF1

Genetic Association Database (GAD)
LEF1
Human Genome Epidemiology (HuGE) Navigator
LEF1
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
LEF1
genes like me logo Genes that share disorders with LEF1: view

No data available for UniProtKB/Swiss-Prot and Genatlas for LEF1 Gene

Publications for LEF1 Gene

  1. Gene for lymphoid enhancer-binding factor 1 (LEF1) mapped to human chromosome 4 (q23-q25) and mouse chromosome 3 near Egf. (PMID: 1783375) Milatovich A. … Francke U. (Genomics 1991) 2 23 67
  2. Wnt/Lef1 signaling acts via Pitx2 to regulate somite myogenesis. (PMID: 19850024) Abu-Elmagd M. … MA1nsterberg A. (Dev. Biol. 2010) 23 67
  3. Human CD1D gene expression is regulated by LEF-1 through distal promoter regulatory elements. (PMID: 20363964) Chen Q.Y. … Lan M.S. (J. Immunol. 2010) 23 67
  4. Lymphoid enhancer factor-1 mediates loading of Pax3 to a promoter harbouring lymphoid enhancer factor-1 binding sites resulting in enhancement of transcription. (PMID: 20006729) Christova T. … Hamel P.A. (Int. J. Biochem. Cell Biol. 2010) 23 67
  5. Inactivation of LEF1 in T-cell acute lymphoblastic leukemia. (PMID: 20124220) Gutierrez A. … Look A.T. (Blood 2010) 23 67

Products for LEF1 Gene

Sources for LEF1 Gene

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