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LEF1 Gene

protein-coding   GIFtS: 66
GCID: GC04M108968

Lymphoid Enhancer-Binding Factor 1

Microbiology & Infectious Diseases Congress
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at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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Aliases
Lymphoid Enhancer-Binding Factor 11 2     TCF102
LEF-12 3     TCF1ALPHA2
TCF1-alpha2 3     TCF7L32
T Cell-Specific Transcription Factor 1-Alpha2 3     

External Ids:    HGNC: 65511   Entrez Gene: 511762   Ensembl: ENSG000001387957   OMIM: 1532455   UniProtKB: Q9UJU23   

Export aliases for LEF1 gene to outside databases

Previous GC identifers: GC04M109394 GC04M109289 GC04M109427 GC04M109326 GC04M109188 GC04M104700


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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Entrez Gene summary for LEF1 Gene:
This gene encodes a transcription factor belonging to a family of proteins that share homology with the high
mobility group protein-1. The protein encoded by this gene can bind to a functionally important site in the
T-cell receptor-alpha enhancer, thereby conferring maximal enhancer activity. This transcription factor is
involved in the Wnt signaling pathway, and it may function in hair cell differentiation and follicle
morphogenesis. Mutations in this gene have been found in somatic sebaceous tumors. This gene has also been linked
to other cancers, including androgen-independent prostate cancer. Alternative splicing results in multiple
transcript variants. (provided by RefSeq, Oct 2009)

GeneCards Summary for LEF1 Gene:
LEF1 (lymphoid enhancer-binding factor 1) is a protein-coding gene. Diseases associated with LEF1 include sebaceous tumors, somatic, and skin pilomatrix carcinoma. GO annotations related to this gene include chromatin binding and sequence-specific DNA binding transcription factor activity. An important paralog of this gene is TCF7L1.

UniProtKB/Swiss-Prot: LEF1_HUMAN, Q9UJU2
Function: Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of
CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and
TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function.
Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by
LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling.
Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the
E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and
increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1
expression and enhances proliferation of pancreatic tumor cells

Gene Wiki entry for LEF1 (Lymphoid enhancer-binding factor 1) Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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Regulatory elements:
   Regulatory transcription factor binding sites in the LEF1 gene promoter:
         C/EBPbeta   Sp1   Nkx2-5   GATA-2   AREB6   Ik-2   SEF-1 (1)   c-Myb   Ik-1   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmids (see all 2): LEF1 promoter sequence
   Search Chromatin IP Primers for LEF1

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat LEF1


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 4q23-q25   Ensembl cytogenetic band:  4q25   HGNC cytogenetic band: 4q23-q25

LEF1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
LEF1 gene location

GeneLoc information about chromosome 4         GeneLoc Exon Structure

GeneLoc location for GC04M108968:  view genomic region     (about GC identifiers)

Start:
108,968,701 bp from pter      End:
109,090,112 bp from pter
Size:
121,412 bases      Orientation:
minus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Cloud-Clone Corp.,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Cloud-Clone Corp., Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, and/or Cloud-Clone Corp.)
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UniProtKB/Swiss-Prot: LEF1_HUMAN, Q9UJU2 (See protein sequence)
Recommended Name: Lymphoid enhancer-binding factor 1  
Size: 399 amino acids; 44201 Da
Subunit: Binds the armadillo repeat of CTNNB1 and forms a stable complex. Interacts with EP300, TLE1 and PIASG (By
similarity). Binds ALYREF/THOC4, MDFI and MDFIC. Interacts with NLK
Secondary accessions: B4DG38 B7Z8E2 E9PDK3 Q3ZCU4 Q9HAZ0
Alternative promoter usage, Alternative splicing: 7 isoforms:  Q9UJU2-1   Q9UJU2-2   Q9UJU2-3   Q9UJU2-4   Q9UJU2-5   Q9UJU2-6   Q9UJU2-7   (No experimental confirmation available)

Explore the universe of human proteins at neXtProt for LEF1: NX_Q9UJU2

Explore proteomics data for LEF1 at MOPED

Post-translational modifications: 

  • Phosphorylated at Thr-155 and/or Ser-166 by NLK. Phosphorylation by NLK at these sites represses LEF1-mediated
    transcriptional activation of target genes of the canonical Wnt signaling pathway1
  • Ubiquitination2 at Lys301, Lys324, Lys347, Lys382
  • Modification sites at PhosphoSitePlus

  • See LEF1 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins (4 alternative transcripts): 
    NP_001124185.1  NP_001124186.1  NP_001159591.1  NP_057353.1  

    ENSEMBL proteins: 
     ENSP00000265165   ENSP00000406176   ENSP00000422840   ENSP00000422334   ENSP00000427459  
     ENSP00000422801   ENSP00000427365   ENSP00000369284  
    Reactome Protein details: Q9UJU2

    LEF1 Human Recombinant Protein Products:

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    OriGene Purified Protein for LEF1
    OriGene Protein Over-expression Lysate for LEF1
    OriGene Custom MassSpec
    OriGene Custom Protein Services for LEF1
    GenScript Custom Purified and Recombinant Proteins Services for LEF1
    Novus Biologicals LEF1 Proteins
    Novus Biologicals LEF1 Lysates
    Browse Sino Biological Recombinant Proteins
    Browse Sino Biological Cell Lysates
    Browse ProSpec Recombinant Proteins
    Cloud-Clone Corp. Proteins for LEF1

    LEF1 Antibody Products:

    EMD Millipore Mono- and Polyclonal Antibodies for the study of LEF1
    R&D Systems Antibodies for LEF1
    Cell Signaling Technology (CST) Antibodies for LEF1  (LEF-1)
    OriGene Antibodies for LEF1
    OriGene Custom Antibody Services for LEF1
    Novus Biologicals LEF1 Antibodies
    Abcam antibodies for LEF1
    Cloud-Clone Corp. Antibodies for LEF1
    ThermoFisher Antibodies for LEF1
    LSBio Antibodies in human, mouse, rat for LEF1

    LEF1 Assay Products:

    EMD Millipore Kits and Assays for the Analysis of LEF1
    OriGene Custom Assay Services for LEF1
    Browse R&D Systems for biochemical assays
    GenScript Custom Assay Services for LEF1
    Browse Enzo Life Sciences for kits & assays
    Cloud-Clone Corp. ELISAs for LEF1
    Cloud-Clone Corp. CLIAs for LEF1


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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    5 InterPro protein domains:
     IPR027397 Catenin_binding_dom
     IPR009071 HMG_box_dom
     IPR028769 LEF1
     IPR024940 TCF/LEF
     IPR013558 CTNNB1-bd_N

    Graphical View of Domain Structure for InterPro Entry Q9UJU2

    ProtoNet protein and cluster: Q9UJU2

    1 Blocks protein domain: IPB013558 CTNNB1 binding

    UniProtKB/Swiss-Prot: LEF1_HUMAN, Q9UJU2
    Domain: Proline-rich and acidic regions are implicated in the activation functions of RNA polymerase II
    transcription factors
    Similarity: Belongs to the TCF/LEF family
    Similarity: Contains 1 HMG box DNA-binding domain


    LEF1 for domains           About GeneDecksing


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Molecular Function:

         UniProtKB/Swiss-Prot Summary: LEF1_HUMAN, Q9UJU2
    Function: Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of
    CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and
    TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function.
    Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by
    LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling.
    Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the
    E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and
    increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1
    expression and enhances proliferation of pancreatic tumor cells

         Genatlas biochemistry entry for LEF1:
    lymphoid enhancer-binding factor,complexing with TCF4 and beta catenin for activation of wingless responsive genes

         Gene Ontology (GO): Selected molecular function terms (see all 20):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000977RNA polymerase II regulatory region sequence-specific DNA binding IDA--
    GO:0001228RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription IMP--
    GO:0003677DNA binding ISS--
    GO:0003682chromatin binding IBA--
    GO:0003700sequence-specific DNA binding transcription factor activity IDA11751639
         
    LEF1 for ontologies           About GeneDecksing


    Phenotypes:
         15 MGI mutant phenotypes (inferred from 3 alleles(MGI details for Lef1):
     adipose tissue  cardiovascular system  craniofacial  embryogenesis  endocrine/exocrine gland 
     growth/size/body  hematopoietic system  immune system  integument  limbs/digits/tail 
     mortality/aging  nervous system  pigmentation  skeleton  vision/eye 

    LEF1 for phenotypes           About GeneDecksing

    Animal Models:
         MGI mouse knock-out Lef1tm1Rug for LEF1

       inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for LEF1
       inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for LEF1

       genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for LEF1
       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for LEF1

    miRNA
    Products:
        
    miRTarBase miRNAs that target LEF1:
    hsa-mir-193b-3p (MIRT016343), hsa-mir-34a-5p (MIRT025380)

    Block miRNA regulation of human, mouse, rat LEF1 using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate LEF1 (see all 51):
    hsa-miR-548j hsa-miR-520e hsa-miR-302d hsa-miR-300 hsa-miR-605 hsa-miR-219-5p hsa-miR-371-5p hsa-miR-507
    SwitchGear 3'UTR luciferase reporter plasmidLEF1 3' UTR sequence
    Inhib. RNA
    Products:
        
    OriGene RNAi products in human, mouse, rat for LEF1
    Predesigned siRNA for gene silencing in human, mouse, rat LEF1

    Gene Editing
    Products:
    DNA2.0 Custom Protein Engineering Service for LEF1

    Clone
    Products:
         
    OriGene clones in human, mouse for LEF1 (see all 24)
    OriGene ORF clones in mouse, rat for LEF1
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector (see all 4): LEF1 (NM_016269)
    Sino Biological Human cDNA Clone for LEF1
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for LEF1
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat LEF1

    Cell Line
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    GenScript Custom overexpressing Cell Line Services for LEF1
    Browse ESI BIO Cell Lines and PureStem Progenitors for LEF1 
    In Situ Assay
    Products:
       

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for LEF1


    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Subcellular locations from UniProtKB/Swiss-Prot
    LEF1_HUMAN, Q9UJU2: Nucleus (By similarity). Note=Found in nuclear bodies upon PIASG binding (By similarity)
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    nucleus4
    cytoskeleton2
    cytosol1
    extracellular1
    plasma membrane1

    Gene Ontology (GO): 5 cellular component terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634nucleus IDA17063141
    GO:0005654nucleoplasm TAS--
    GO:0005667transcription factor complex IDA11751639
    GO:0005737cytoplasm IDA19402906
    GO:0032993protein-DNA complex IDA10825188

    LEF1 for ontologies           About GeneDecksing


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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    SuperPaths for LEF1 About   (see all 27)                                                                                              See pathways by source

    SuperPathContained pathways About
    1Wnt signaling pathway (KEGG)
    Wnt signaling pathway0.40
    Wnt Signaling Pathway NetPath0.37
    Wnt Signaling Pathways0.40
    2Endometrial cancer
    Endometrial cancer0.45
    Colorectal cancer0.42
    Signal transduction PTEN pathway0.45
    Acute myeloid leukemia0.40
    3Hypertrophic cardiomyopathy (HCM)
    Arrhythmogenic right ventricular cardiomyopathy (ARVC)0.54
    Arrhythmogenic right ventricular cardiomyopathy0.54
    4beta-catenin independent WNT signaling
    beta-catenin independent WNT signaling0.79
    Ca2+ pathway0.43
    Signaling by Wnt0.79
    5Non-Canonical Wnt Pathway
    Non-Canonical Wnt Pathway0.53
    Canonical Wnt Pathway0.53

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways

    1 R&D Systems Pathway for LEF1
        Wnt Signaling Pathways

    Selected Downloadable PowerPoint Slides of GeneGlobe Pathway Central Maps for LEF1 (see all 9)
        Factors Promoting Cardiogenesis in Vertebrates
    Molecular Mechanisms of Cancer
    Colorectal Cancer Metastasis
    Glioblastoma Multiforme
    Epithelial Adherens Junctions

    1 Cell Signaling Technology (CST) Pathway for LEF1
        Wnt / Hedgehog / Notch

    3 GeneGo (Thomson Reuters) Pathways for LEF1
        Cytoskeleton remodeling Integrin outside-in signaling
    Signal transduction PTEN pathway
    Cell adhesion Tight junctions

    Selected BioSystems Pathways for LEF1 (see all 8)
        TGF Beta Signaling Pathway
    Wnt Signaling Pathway and Pluripotency
    TGF-beta Receptor Signaling Pathway
    Wnt Signaling Pathway NetPath
    Delta-Notch Signaling Pathway

    2 Sino Biological Pathways for LEF1
        Canonical Wnt Pathway
    Non-Canonical Wnt Pathway

    4 Reactome Pathways for LEF1
        deactivation of the beta-catenin transactivating complex
    Ca2+ pathway
    binding of TCF/LEF:CTNNB1 to target gene promoters
    formation of the beta-catenin:TCF transactivating complex


    Selected Kegg Pathways  (Kegg details for LEF1) (see all 12):
        Wnt signaling pathway
    Hippo signaling pathway
    Adherens junction
    Melanogenesis
    Pathways in cancer


    LEF1 for pathways           About GeneDecksing

        Pathway & Disease-focused RT2 Profiler PCR Arrays including LEF1: 
              WNT Signaling Targets in human mouse rat
              WNT Signaling Pathway in human mouse rat
              Stem Cell Signaling in human mouse rat
              Liver Cancer in human mouse rat
              Hematopoiesis in human mouse rat

    Interactions:

        GeneGlobe Interaction Network for LEF1

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    Selected Interacting proteins for LEF1 (Q9UJU21, 2, 3 ENSP000002651654) via UniProtKB, MINT, STRING, and/or I2D (see all 108)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    IGF1RP080691, 2EBI-926131,EBI-475981 MINT-8329899 MINT-8331110 MINT-8329627 MINT-8329874 MINT-8329861
    CTNNB1P352221, 2, 3, ENSP000003444564EBI-926131,EBI-491549 MINT-8329899 MINT-8329627 I2D: score=10 STRING: ENSP00000344456
    SMAD2Q157963, ENSP000002621604I2D: score=4 STRING: ENSP00000262160
    SMAD3P840223, ENSP000003329734I2D: score=4 STRING: ENSP00000332973
    ALX4Q9H1613, ENSP000003327444I2D: score=3 STRING: ENSP00000332744
    About this table

    Gene Ontology (GO): Selected biological process terms (see all 75):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000122negative regulation of transcription from RNA polymerase II promoter IEA--
    GO:0001569patterning of blood vessels IBA--
    GO:0001649osteoblast differentiation IEP--
    GO:0001755neural crest cell migration IBA--
    GO:0001756somitogenesis IBA--

    LEF1 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Browse Tocris compounds for LEF1

    6 Novoseek inferred chemical compound relationships for LEF1 gene    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    glycogen 42.7 2 15684397 (1), 14520463 (1)
    tyrosine 0 1 11401329 (1)
    matrigel 0 3 16142310 (1)
    alanine 0 1 12556497 (1)
    cisplatin 0 4 17961652 (2)
    serine 0 1 14594802 (1)



    LEF1 for compounds           About GeneDecksing



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    Conferences by KenesGroup, exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, and/or QIAGEN )
    About This Section

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    REFSEQ mRNAs for LEF1 gene (4 alternative transcripts): 
    NM_001130713.2  NM_001130714.2  NM_001166119.1  NM_016269.4  

    Selected Ensembl transcripts including schematic representations, and UCSC links where relevant (see all 21):
    ENST00000265165(uc011cfj.1 uc011cfk.2) ENST00000503879 ENST00000504426
    ENST00000505379 ENST00000514444 ENST00000505297 ENST00000510135 ENST00000438313
    ENST00000507470 ENST00000509428 ENST00000512407 ENST00000510624 ENST00000506680
    ENST00000505328 ENST00000504775(uc003hyw.1) ENST00000504950 ENST00000515500
    ENST00000510717
    Congresses - knowledge worth sharing:
    European Congress of Clinical Microbiology and Infectious Diseases (ECCMID) 10 - 13 May 2014

    miRNA
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    Selected qRT-PCR Assays for microRNAs that regulate LEF1 (see all 51):
    hsa-miR-548j hsa-miR-520e hsa-miR-302d hsa-miR-300 hsa-miR-605 hsa-miR-219-5p hsa-miR-371-5p hsa-miR-507
    SwitchGear 3'UTR luciferase reporter plasmidLEF1 3' UTR sequence
    Inhib. RNA
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    Predesigned siRNA for gene silencing in human, mouse, rat LEF1
    Clone
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    OriGene clones in human, mouse for LEF1 (see all 24)
    OriGene ORF clones in mouse, rat for LEF1
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector (see all 4): LEF1 (NM_016269)
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for LEF1
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat LEF1
    Primer
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    OriGene qPCR primer pairs and template standards for LEF1
    OriGene qSTAR qPCR primer pairs in human, mouse for LEF1
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat LEF1
      QuantiTect SYBR Green Assays in human, mouse, rat LEF1
      QuantiFast Probe-based Assays in human, mouse, rat LEF1

    Selected AceView cDNA sequences (see all 193):

    BX421802 AI332496 BQ215285 CR597256 AL049409 CR604746 BP429129 BQ229694 
    BX280486 AA310462 BU687283 AI140654 BU189309 AI697371 BF197032 BQ184671 
    AF086339 AA353660 BQ228886 BU189289 BG505050 BG390921 AW028292 AW237073 

    GeneLoc Exon Structure

    Selected Alternative Splicing Database (ASD) splice patterns (SP) for LEF1 (see all 11)    About this scheme

    ExUns: 1a · 1b ^ 2 ^ 3 ^ 4 ^ 5a · 5b ^ 6 ^ 7 ^ 8 ^ 9a · 9b · 9c ^ 10 ^ 11 ^ 12a · 12b ^ 13a · 13b ^ 14a · 14b ^ 15 ^ 16 ^ 17 ^ 18 ^ 19a ·
    SP1:                                                  -                             -     -     -     -     -     -                             -     -     -   
    SP2:                                            -     -                             -     -     -     -     -     -                       -     -     -     -   
    SP3:                                                  -                             -     -     -     -     -     -                       -     -     -     -   
    SP4:                                                                                            -           -                                   -     -     -   
    SP5:                                                                                      -     -     -     -     -                             -     -     -   

    ExUns: 19b · 19c · 19d · 19e · 19f · 19g
    SP1:                                    
    SP2:                                    
    SP3:                                    
    SP4:                                    
    SP5:                                    


    ECgene alternative splicing isoforms for LEF1

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
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    LEF1 expression in normal human tissues (normalized intensities)      LEF1 embryonic expression: see
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: GAAGAGTTCC
    LEF1 Expression
    About this image


    LEF1 expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database
     selected tissues (see all 23) fully expand
     
     Epithelial Cells
             Fetal Precortex Cells Hair Shaft
     
     Hair (Integumentary System)    fully expand to see all 4 entries
             Fetal Precortex Cells Hair Shaft
     
     Tooth
             Dental Placode Cells Dental Placode
     
     Ectoderm (Gastrulation Derivatives)    fully expand to see all 2 entries
             Apical Ectodermal Ridge Cells Limb Ectoderm
     
     Neural Tube (Nervous System)    fully expand to see all 4 entries
             Metencephalon
             Floor plate-like cells
    LEF1 Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    LEF1 Protein Expression

    UniProtKB/Swiss-Prot: LEF1_HUMAN, Q9UJU2
    Tissue specificity: Detected in thymus. Not detected in normal colon, but highly expressed in colon cancer
    biopsies and colon cancer cell lines. Expressed in several pancreatic tumors and weakly expressed in normal
    pancreatic tissue. Isoforms 1 and 5 are detected in several pancreatic cell lines

        Pathway & Disease-focused RT2 Profiler PCR Arrays including LEF1: 
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              Liver Cancer in human mouse rat
              Hematopoiesis in human mouse rat

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    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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    This gene was present in the common ancestor of animals.

    Orthologs for LEF1 gene from Selected species (see all 16)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Lef11 , 5 lymphoid enhancer binding factor 11, 5 91.52(n)1
    97.73(a)1
      3 (60.78 cM)5
    168421  NM_010703.41  NP_034833.21 
     1311104715 
    chicken
    (Gallus gallus)
    Aves LEF11 lymphoid enhancer-binding factor 1 85.25(n)
    94.96(a)
      395865  NM_205013.2  NP_990344.1 
    lizard
    (Anolis carolinensis)
    Reptilia LEF16
    lymphoid enhancer-binding factor 1
    93(a)
    1 ↔ 1
    GL343384.1(157198-293842)
    African clawed frog
    (Xenopus laevis)
    Amphibia LOC3982382 lymphoid enhancer factor XLEF-1 80.71(n)    AF063831.1 
    zebrafish
    (Danio rerio)
    Actinopterygii lef12 lymphocyte enhancer binding factor 1 77.72(n)   30701  AF136454.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta pan3 heart development transcription factor 39(a)     --
    worm
    (Caenorhabditis elegans)
    Secernentea pop-16
    Protein POP-1 (pop-1) mRNA, complete cds
    24(a)
    1 → many
    I(2823468-2830293) WBGene00004077


    ENSEMBL Gene Tree for LEF1 (if available)
    TreeFam Gene Tree for LEF1 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
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    Paralogs for LEF1 gene
    TCF7L12  TCF72  TCF7L22  
    3 SIMAP similar genes for LEF1 using alignment to 5 protein entries:     LEF1_HUMAN (see all proteins):
    TCF7    TCF7L1    TCF7L2

    LEF1 for paralogs           About GeneDecksing



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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    Selected SNPs for LEF1 (see all 2254)    About this table    
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 4 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    VAR_0359354
    A colorectal cancer sample4--see VAR_0359352 G R mis40--------
    rs106768701,2
    C--104738143(+) TTGAC-/TCTA  
            
    TCTAT
    4 -- int10--------
    rs9247241,2
    C,H--104797573(+) CAGGTC/TTCATA 4 -- int116Minor allele frequency- T:0.00NS EA NA WA CSA 794
    rs58609021,2
    C,F--104800934(+) AAAAAC/-ATTTA 4 -- int1 trp32Minor allele frequency- -:0.50NA CSA 4
    rs30347731,2
    C--104809828(+) TATAT-/AC/ATACA
    CAC
    /TACACA
    ACACA
    8 -- int1 cds10--------
    rs21070281,2
    C,F,A,H--108763264(+) ATACAG/ACTCAG 4 -- ds500124Minor allele frequency- A:0.20NS EA NA WA CSA 2468
    rs1922385231,2
    --108763376(+) TATGAA/CGAGAG 4 -- ds50010--------
    rs2002289621,2
    C--108763386(+) GGCGGG/TACCTG 4 -- ds50010--------
    rs558763221,2
    C,F--108763396(+) GTGGAC/ATTTCA 4 -- ds50018Minor allele frequency- A:0.24NA WA EA 368
    rs1840773661,2
    --108763532(+) GGGAGA/TGGCCC 4 -- ds50010--------

    HapMap Linkage Disequilibrium report for LEF1 (108968701 - 109090112 bp)

    Structural Variations
         Database of Genomic Variants (DGV) 3 variations for LEF1:    About this table    
    Variant IDTypeSubtypePubMed ID
    esv275379CNV Loss21479260
    nsv4458CNV Loss18451855
    nsv830032CNV Gain17160897

    Site Specific Mutation Identification with PCR Assays
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    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Conferences by KenesGroup, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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    OMIM gene information: 153245    OMIM disorders: --

    Selected diseases for LEF1 (see all 54):    About MalaCards
    sebaceous tumors, somatic    skin pilomatrix carcinoma    odontoma    pilomatrixoma
    cytogenetically normal acute myeloid leukemia    severe congenital neutropenia    neutropenia    peripheral t-cell lymphoma
    craniopharyngioma    familial adenomatous polyposis    anaplastic large cell lymphoma    prostate cancer
    ectodermal dysplasia    microphthalmia    prostatitis    myeloid leukemia
    alcohol dependence    glioblastoma multiforme    thyroid cancer    graves' disease

    2 diseases from the University of Copenhagen DISEASES database for LEF1:
    Skin pilomatrix carcinoma     Pilomatrixoma

    LEF1 for disorders           About GeneDecksing

    Selected Novoseek inferred disease relationships for LEF1 gene (see all 26)    About this table

    Disease   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    neutropenia, severe congenital 73.4 2 17360796 (1), 19620402 (1)
    familial adenomatous polyposis 60.8 4 10085258 (1), 10318916 (1), 16378739 (1), 14520463 (1)
    pilomatricoma 57 3 16441405 (1), 10192393 (1), 15140206 (1)
    colon carcinoma 47.2 10 15979100 (2), 16378739 (1), 19067737 (1), 11401329 (1)
    colon cancer 40.1 12 12582159 (2), 16809766 (2), 14675489 (1), 9419974 (1) (see all 9)
    cancer 28.1 14 9419974 (2), 15756419 (2), 11326276 (1), 12525640 (1) (see all 10)
    lymphoma t-cell peripheral 22.6 2 12707037 (2)
    leukemia lymphocytic chronic 20.1 1 15756419 (1)
    anaplastic large cell lymphoma 18.7 2 12707037 (1)
    blast crisis 18.5 1 17361218 (1)

    Genetic Association Database (GAD): LEF1
    Human Genome Epidemiology (HuGE) Navigator: LEF1 (8 documents)

    Export disorders for LEF1 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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    PubMed articles for LEF1 gene, integrated from 10 sources (see all 337):
    (articles sorted by number of sources associating them with LEF1)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Lef-1 isoforms regulate different target genes and reduce cellular adhesion. (PubMed id 19653274)1, 2, 9 Jesse S.... Menke A. (Int. J. Cancer 2010)
    2. Gene for lymphoid enhancer-binding factor 1 (LEF1) mapped to human chromosome 4 (q23-q25) and mouse chromosome 3 near Egf. (PubMed id 1783375)1, 3, 9 Milatovich A....Francke U. (Genomics 1991)
    3. ALY, a context-dependent coactivator of LEF-1 and AML-1, is required for TCRalpha enhancer function. (PubMed id 9119228)1, 2, 9 Bruhn L.... Grosschedl R. (Genes Dev. 1997)
    4. A thymus-specific member of the HMG protein family regulates the human T cell receptor C alpha enhancer. (PubMed id 2010090)1, 2, 9 Waterman M.L.... Jones K.A. (Genes Dev. 1991)
    5. Beta-catenin-sensitive isoforms of lymphoid enhancer factor-1 are selectively expressed in colon cancer. (PubMed id 11326276)1, 2, 9 Hovanes K.... Waterman M.L. (Nat. Genet. 2001)
    6. The human LEF-1 gene contains a promoter preferentially active in lymphocytes and encodes multiple isoforms derived from alternative splicing. (PubMed id 10756202)1, 2, 9 Hovanes K.... Waterman M.L. (Nucleic Acids Res. 2000)
    7. Regulation of lymphoid enhancer factor 1/T-cell factor by mitogen- activated protein kinase-related Nemo-like kinase-dependent phosphorylation in Wnt/beta-catenin signaling. (PubMed id 12556497)1, 2, 9 Ishitani T.... Matsumoto K. (Mol. Cell. Biol. 2003)
    8. A genome-wide association study identified AFF1 as a susceptibility locus for systemic lupus eyrthematosus in Japanese. (PubMed id 22291604)1, 4 Okada Y....Yamamoto K. (PLoS Genet. 2012)
    9. Pathway-based approaches to imaging genetics association studies: Wnt signaling, GSK3beta substrates and major depression. (PubMed id 20219685)1, 4 Inkster B....Matthews P.M. (Neuroimage 2010)
    10. A systematic gene-based screen of chr4q22-q32 identifies association of a novel susceptibility gene, DKK2, with the quantitative trait of alcohol dependence symptom counts. (PubMed id 20332099)1, 4 Kalsi G....Riley B.P. (Hum. Mol. Genet. 2010)

    (in PubMed, OMIM, and NCBI Bookshelf)
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     ANDOR
    Aliases
    Disorders
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Entrez Gene: 51176 HGNC: 6551 AceView: LEF1 Ensembl:ENSG00000138795 euGenes: HUgn51176
    ECgene: LEF1 Kegg: 51176 H-InvDB: LEF1

    (According to HUGE)
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      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
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    NameDescription
    PharmGKB entry for LEF1 Pharmacogenomics, SNPs, Pathways
    ATLAS Chromosomes in Cancer entry for LEF1 Genetics and Cytogenetics in Oncology and Haematology

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
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    Patent Information for LEF1 gene:
    Search GeneIP for patents involving LEF1

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript, and ESI BIO, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
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