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Aliases for LCMT2 Gene

Aliases for LCMT2 Gene

  • Leucine Carboxyl Methyltransferase 2 2 3 4
  • TRNA(Phe) (7-(3-Amino-3-(Methoxycarbonyl)Propyl)Wyosine(37)-N)-Methoxycarbonyltransferase 3 4
  • TRNA(Phe) (7-(3-Amino-3-Carboxypropyl)Wyosine(37)-O)-Methyltransferase 3 4
  • TRNA-YW Synthesizing Protein 4 2 3
  • TRNA YW-Synthesizing Protein 4 3 4
  • TYW4 3 4
  • P21WAF1/CIP1 Promoter-Interacting Protein 3
  • TRNA-Wybutosine-Synthesizing Protein 4 3
  • EC 2.1.1.290 4
  • EC 2.3.1.231 4
  • KIAA0547 4
  • PPM2 3

External Ids for LCMT2 Gene

Previous GeneCards Identifiers for LCMT2 Gene

  • GC15M036514
  • GC15M041199
  • GC15M041336
  • GC15M041407
  • GC15M043619
  • GC15M020468

Summaries for LCMT2 Gene

Entrez Gene Summary for LCMT2 Gene

  • The protein encoded by this intronless gene belongs to the highly variable methyltransferase superfamily. This gene is the inferred homolog of the Saccharomyces cerevisiae carboxymethyltransferase gene PPM2 that is essential for the synthesis of the hypermodified guanosine Wybutosine (yW). [provided by RefSeq, Jul 2008]

GeneCards Summary for LCMT2 Gene

LCMT2 (Leucine Carboxyl Methyltransferase 2) is a Protein Coding gene. Among its related pathways are Gene Expression and tRNA modification in the nucleus and cytosol. GO annotations related to this gene include methyltransferase activity. An important paralog of this gene is LCMT1.

UniProtKB/Swiss-Prot for LCMT2 Gene

  • Probable S-adenosyl-L-methionine-dependent methyltransferase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3-position adjacent to the anticodon of eukaryotic phenylalanine tRNA (By similarity). May methylate the carboxyl group of leucine residues to form alpha-leucine ester residues.

Gene Wiki entry for LCMT2 Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for LCMT2 Gene

Genomics for LCMT2 Gene

Regulatory Elements for LCMT2 Gene

Epigenetics Products

  • DNA Methylation CpG Assay Predesigned for Pyrosequencing in human,mouse,rat

Genomic Location for LCMT2 Gene

Chromosome:
15
Start:
43,323,649 bp from pter
End:
43,330,622 bp from pter
Size:
6,974 bases
Orientation:
Minus strand

Genomic View for LCMT2 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
GeneLoc Logo Gene Density

RefSeq DNA sequence for LCMT2 Gene

Proteins for LCMT2 Gene

  • Protein details for LCMT2 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    O60294-TYW4_HUMAN
    Recommended name:
    tRNA wybutosine-synthesizing protein 4
    Protein Accession:
    O60294
    Secondary Accessions:
    • Q4JFT6
    • Q96B55
    • Q9NR10

    Protein attributes for LCMT2 Gene

    Size:
    686 amino acids
    Molecular mass:
    75602 Da
    Quaternary structure:
    • Interacts with RNF144B/IBRDC2.
    SequenceCaution:
    • Sequence=BAA25473.2; Type=Erroneous initiation; Evidence={ECO:0000305};

neXtProt entry for LCMT2 Gene

Proteomics data for LCMT2 Gene at MOPED

Post-translational modifications for LCMT2 Gene

  • Ubiquitination at Lys 24, Lys 356, and Lys 401
  • Modification sites at PhosphoSitePlus

Other Protein References for LCMT2 Gene

ENSEMBL proteins:
REFSEQ proteins:

Antibody Products

No data available for DME Specific Peptides for LCMT2 Gene

Domains & Families for LCMT2 Gene

Protein Domains for LCMT2 Gene

Graphical View of Domain Structure for InterPro Entry

O60294

UniProtKB/Swiss-Prot:

TYW4_HUMAN :
  • Belongs to the methyltransferase superfamily. LCMT family.
Family:
  • Belongs to the methyltransferase superfamily. LCMT family.
genes like me logo Genes that share domains with LCMT2: view

No data available for Gene Families for LCMT2 Gene

Function for LCMT2 Gene

Molecular function for LCMT2 Gene

UniProtKB/Swiss-Prot CatalyticActivity:
S-adenosyl-L-methionine + 7-((3S)-3-amino-3-carboxypropyl)wyosine(37) in tRNA(Phe) = S-adenosyl-L-homocysteine + 7-((3S)-3-amino-3-(methoxycarbonyl)propyl)wyosine(37) in tRNA(Phe)
UniProtKB/Swiss-Prot CatalyticActivity:
S-adenosyl-L-methionine + 7-((3S)-3-amino-3-(methoxycarbonyl)propyl)wyosine(37) in tRNA(Phe) + CO(2) = S-adenosyl-L-homocysteine + wybutosine(37) in tRNA(Phe)
UniProtKB/Swiss-Prot Function:
Probable S-adenosyl-L-methionine-dependent methyltransferase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3-position adjacent to the anticodon of eukaryotic phenylalanine tRNA (By similarity). May methylate the carboxyl group of leucine residues to form alpha-leucine ester residues.

Enzyme Numbers (IUBMB) for LCMT2 Gene

Gene Ontology (GO) - Molecular Function for LCMT2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005515 protein binding IPI 12853982
GO:0008168 methyltransferase activity --
GO:0008175 tRNA methyltransferase activity IBA --
GO:0008757 S-adenosylmethionine-dependent methyltransferase activity IBA --
genes like me logo Genes that share ontologies with LCMT2: view

Phenotypes for LCMT2 Gene

genes like me logo Genes that share phenotypes with LCMT2: view

Animal Model Products

CRISPR Products

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for LCMT2

In Situ Assay Products

Flow Cytometry Products

No data available for Animal Models , Transcription Factor Targets and HOMER Transcription for LCMT2 Gene

Localization for LCMT2 Gene

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for LCMT2 Gene COMPARTMENTS Subcellular localization image for LCMT2 gene
Compartment Confidence
nucleus 2

Gene Ontology (GO) - Cellular Components for LCMT2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005737 cytoplasm TAS --
GO:0005829 cytosol IBA --
genes like me logo Genes that share ontologies with LCMT2: view

No data available for Subcellular locations from UniProtKB/Swiss-Prot for LCMT2 Gene

Pathways & Interactions for LCMT2 Gene

genes like me logo Genes that share pathways with LCMT2: view

Pathways by source for LCMT2 Gene

UniProtKB/Swiss-Prot O60294-TYW4_HUMAN

  • Pathway: tRNA modification; wybutosine-tRNA(Phe) biosynthesis

Interacting Proteins for LCMT2 Gene

Gene Ontology (GO) - Biological Process for LCMT2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006400 tRNA modification TAS --
GO:0008033 tRNA processing TAS --
GO:0010467 gene expression TAS --
GO:0030488 tRNA methylation IBA --
GO:0031591 wybutosine biosynthetic process IBA --
genes like me logo Genes that share ontologies with LCMT2: view

No data available for SIGNOR curated interactions for LCMT2 Gene

Drugs & Compounds for LCMT2 Gene

(2) Drugs for LCMT2 Gene - From: HMDB and DrugBank

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
L-Leucine Approved Nutra Target 0
S-Adenosylmethionine Approved Nutra 0
genes like me logo Genes that share compounds with LCMT2: view

Transcripts for LCMT2 Gene

mRNA/cDNA for LCMT2 Gene

(1) REFSEQ mRNAs :
(4) Additional mRNA sequences :
(94) Selected AceView cDNA sequences:
(2) Ensembl transcripts including schematic representations, and UCSC links where relevant :

Unigene Clusters for LCMT2 Gene

Leucine carboxyl methyltransferase 2:
Representative Sequences:

CRISPR Products

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for LCMT2

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for LCMT2 Gene

No ASD Table

Relevant External Links for LCMT2 Gene

GeneLoc Exon Structure for
LCMT2
ECgene alternative splicing isoforms for
LCMT2

Expression for LCMT2 Gene

mRNA expression in normal human tissues for LCMT2 Gene

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MOPED, and MaxQB for LCMT2 Gene



SOURCE GeneReport for Unigene cluster for LCMT2 Gene Hs.200596

genes like me logo Genes that share expression patterns with LCMT2: view

Primer Products

In Situ Assay Products

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , mRNA differential expression in normal tissues , Protein differential expression in normal tissues , mRNA Expression by UniProt/SwissProt and Protein tissue co-expression partners for LCMT2 Gene

Orthologs for LCMT2 Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for LCMT2 Gene

Organism Taxonomy Gene Similarity Type Details
cow
(Bos Taurus)
Mammalia LCMT2 35
  • 85.74 (n)
  • 81.75 (a)
LCMT2 36
  • 82 (a)
OneToOne
dog
(Canis familiaris)
Mammalia LCMT2 35
  • 86.91 (n)
  • 83.21 (a)
LCMT2 36
  • 83 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Lcmt2 35
  • 80.86 (n)
  • 77.84 (a)
Lcmt2 16
Lcmt2 36
  • 78 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Lcmt2 35
  • 80.03 (n)
  • 77.26 (a)
chimpanzee
(Pan troglodytes)
Mammalia LCMT2 36
  • 84 (a)
OneToOne
chicken
(Gallus gallus)
Aves LCMT2 35
  • 57.53 (n)
  • 52.25 (a)
LCMT2 36
  • 51 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia LCMT2 36
  • 52 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia lcmt2 35
  • 53.98 (n)
  • 46.9 (a)
zebrafish
(Danio rerio)
Actinopterygii lcmt2 35
  • 52.57 (n)
  • 47.73 (a)
lcmt2 36
  • 46 (a)
OneToOne
A. gosspyii yeast
(Ashbya gossypii)
Saccharomycetes AGOS_ADL194W 35
  • 43.48 (n)
  • 34.06 (a)
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes PPM2 35
  • 41.63 (n)
  • 29.95 (a)
PPM2 36
  • 20 (a)
OneToMany
PPM2 38
bread mold
(Neurospora crassa)
Ascomycetes NCU05392 35
  • 43.21 (n)
  • 33.82 (a)
fission yeast
(Schizosaccharomyces pombe)
Schizosaccharomycetes SPBC19C7.08c 35
  • 37.74 (n)
  • 28.16 (a)
sea squirt
(Ciona savignyi)
Ascidiacea CSA.10693 36
  • 29 (a)
OneToOne
Species with no ortholog for LCMT2:
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • oppossum (Monodelphis domestica)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • platypus (Ornithorhynchus anatinus)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for LCMT2 Gene

ENSEMBL:
Gene Tree for LCMT2 (if available)
TreeFam:
Gene Tree for LCMT2 (if available)

Paralogs for LCMT2 Gene

Paralogs for LCMT2 Gene

genes like me logo Genes that share paralogs with LCMT2: view

Variants for LCMT2 Gene

Sequence variations from dbSNP and Humsavar for LCMT2 Gene

SNP ID Clin Chr 15 pos Sequence Context AA Info Type MAF
rs7048 -- 43,327,875(-) ACTTG(G/T)AGGAC utr-variant-3-prime
rs484029 -- 43,327,362(-) TTGAC(A/C/G/T)ACATG downstream-variant-500B
rs514438 -- 43,328,245(-) ACTAC(A/C/G/T)GAAAA utr-variant-3-prime
rs2577058 -- 43,327,714(-) ctgtt(C/T)tgtgc downstream-variant-500B
rs12324573 -- 43,327,753(+) AACAT(G/T)ATTAT downstream-variant-500B

Structural Variations from Database of Genomic Variants (DGV) for LCMT2 Gene

Variant ID Type Subtype PubMed ID
nsv1512 CNV Loss 18451855

Variation tolerance for LCMT2 Gene

Residual Variation Intolerance Score: 43.15% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 3.29; 53.09% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for LCMT2 Gene

HapMap Linkage Disequilibrium report
LCMT2

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for LCMT2 Gene

Disorders for LCMT2 Gene

Relevant External Links for LCMT2

Atlas of Genetics and Cytogenetics in Oncology and Haematology:
LCMT2
genes like me logo Genes that share disorders with LCMT2: view

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for LCMT2 Gene

Publications for LCMT2 Gene

  1. Ribonucleome analysis identified enzyme genes responsible for wybutosine synthesis. (PMID: 17150819) Noma A. … Suzuki T. (Nucleic Acids Symp Ser (Oxf) 2006) 2 67
  2. Prediction of the coding sequences of unidentified human genes. IX. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro. (PMID: 9628581) Nagase T. … Ohara O. (DNA Res. 1998) 2 67
  3. The BioPlex Network: A Systematic Exploration of the Human Interactome. (PMID: 26186194) Huttlin E.L. … Gygi S.P. (Cell 2015) 67
  4. Temporal proteomics of NGF-TrkA signaling identifies an inhibitory role for the E3 ligase Cbl-b in neuroblastoma cell differentiation. (PMID: 25921289) Emdal K.B. … Olsen J.V. (Sci Signal 2015) 67
  5. Panorama of ancient metazoan macromolecular complexes. (PMID: 26344197) Wan C. … Emili A. (Nature 2015) 67

Products for LCMT2 Gene

Sources for LCMT2 Gene

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