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LCK Gene

protein-coding   GIFtS: 75

GC01P032489
lymphocyte-specific protein tyrosine kinase
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    

Aliases &
Descriptions
for LCK

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section
Aliases
EC 2.7.10.2 3
LSK 3
YT16 2
p56-LCK 3
p56lck 2
pp58lck 2
Descriptions
Lymphocyte cell-specific protein-tyrosine kinase 3
T cell-specific protein-tyrosine kinase 3
T-lymphocyte specific protein tyrosine kinase p56lck 2
lymphocyte-specific protein tyrosine kinase 2
p56(LSTRA) protein-tyrosine kinase 2
protein tyrosine kinase 2
proto-oncogene tyrosine-protein kinase LCK 2
External Ids
HGNC: 65241
Entrez Gene: 39322
UniProtKB: P062393
Ensembl: ENSG000001828667
Search outside databases for aliases for LCK gene

Previous GC identifers: GC01U990096 GC01P032143 GC01P032219 GC01P032386

Summaries
for LCK

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

 EntrezGene summary for LCK:
This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein is
a key signaling molecule in the selection and maturation of developing T-cells. It contains
N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which
are involved in mediating protein-protein interactions with phosphotyrosine-containing and
proline-rich motifs, respectively. The protein localizes to the plasma membrane and
pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other
signaling molecules. Multiple alternatively spliced variants, encoding the same protein, have been
described. [provided by RefSeq]

UniProtKB/Swiss-Prot: LCK_HUMAN, P06239
Function: Tyrosine kinase that plays an essential role for the selection and maturation of
developing T-cell in the thymus and in mature T-cell function. Is constitutively associated with
the cytoplasmic portions of the CD4 and CD8 surface receptors and plays a key role in T-cell
antigen receptor(TCR)-linked signal transduction pathways. Association of the TCR with a peptide
antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I
molecules, respectively, and thereby recruits the associated LCK to the vicinity of the TCR/CD3
complex. LCK then phosphorylates tyrosines residues within the immunoreceptor tyrosines-based
activation motifs (ITAMs) in the cytoplasmic tails of the TCRgamma chains and CD3 subunits,
initiating the TCR/CD3 signaling pathway. In addition, contributes to signaling by other receptor
molecules. Associates directly with the cytoplasmic tail of CD2, and upon engagement of the CD2
molecule, LCK undergoes hyperphosphorylation and activation. Also plays a role in the IL2
receptor-linked signaling pathway that controls T-cell proliferative response. Binding of IL2 to
its receptor results in increased activity of LCK. Is expressed at all stages of thymocyte
development and is required for the regulation of maturation events that are governed by both
pre-TCR and mature alpha beta TCR

Gene Wiki entry for LCK (Lck)

Genomic Location
for LCK

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

 Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the LCK gene  

Entrez Gene cytogenetic band: 1p34.3   Ensembl cytogenetic band:  1p35.1   HGNC cytogenetic band: 1p34.3

LCK Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 1         GeneLoc Exon Structure

GeneLoc location for GC01P032489:     (about GC identifiers)

Start:
 32,489,427 bp from pter
End:
 32,524,353 bp from pter
Size:
 34,927 bases
Orientation:
 plus strand
RefSeq DNA sequence:
NC_000001.9  NT_032977.8  

Proteins
for LCK

(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

 UniProtKB/Swiss-Prot: LCK_HUMAN, P06239 (See protein sequence)
Recommended Name: Proto-oncogene tyrosine-protein kinase LCK  
Size: 509 amino acids; 58001 Da
Subunit: Binds to the cytoplasmic domain of cell surface receptors, such as CD2, CD4, CD5, CD8,
CD44, CD45 and CD122. Also binds to effector molecules, such as PI4K, VAV1, RASA1, FYB and to
other protein kinases including CDC2, RAF1, ZAP70 and SYK. Binds to phosphatidylinositol 3'-kinase
(PI3K) from T-lymphocytes through its SH3 domain and to the tyrosine phosphorylated form of
KHDRBS1/p70 through its SH2 domain. Binds to HIV-1 Nef through its SH3 domain. This interaction
inhibits its tyrosine-kinase activity. Interacts with SQSTM1. Interacts with phosphorylated LIME1.
Interacts with CBLB and PTPRH
Subcellular location: Cytoplasm. Cell membrane; Lipid-anchor; Cytoplasmic side. Note=Present in
lipid rafts in an unactive form
Mass spectrometry: Mass=57869.42; Method=MALDI; Range=2-509; Source=PubMed:11840567;
PDB structures from and Proteopedia :
1BHF (3D)    1BHH (3D)    1CWD (3D)    1CWE (3D)    1FBZ (3D)    1H92 (3D)    1IJR (3D)    1KIK (3D)    
1LCJ (3D)    1LCK (3D)    1LKK (3D)    1LKL (3D)    1Q68 (3D)    1Q69 (3D)    1QPC (3D)    1QPD (3D)    
1QPE (3D)    1QPJ (3D)    1X27 (3D)    2IIM (3D)    2OF2 (3D)    2OF4 (3D)    2OFU (3D)    2OFV (3D)    
2OG8 (3D)    2PL0 (3D)    2ZM1 (3D)    2ZM4 (3D)    2ZYB (3D)    3B2W (3D)    3BRH (3D)    3BYM (3D)    
3BYO (3D)    3BYS (3D)    3BYU (3D)    3LCK (3D)    
Secondary accessions: P07100 Q12850 Q13152 Q5TDH8 Q5TDH9 Q7RTZ3 Q96DW4 Q9NYT8
Alternative splicing: 3 isoforms:  P06239-1   P06239-2   P06239-3   

Post-translational modifications:

  • Phosphorylated on Tyr-394, which increases enzymatic activity (By similarity). Phosphorylated on
    Tyr-505, which decreases activity1
  • View phosphorylation sites using PhosphoSite2


    • REFSEQ proteins (2 alternative transcripts): 
    NP_001036236.1  NP_005347.3  


    ENSEMBL proteins: 
    ENSP00000362663 ENSP00000362665 ENSP00000362658 ENSP00000337825 ENSP00000348194 ENSP00000328213 
    ENSP00000381387 


    Human Recombinant Proteins 
    Invitrogen human recombinant proteins for LCK
    Millipore Purified and/or Recombinant LCK Protein
    Sigma-Aldrich Proteins for LCK  
    R&D Systems Recombinant & Natural Proteins for LCK
    Enzo Life Sciences proteins for LCK
    Recombinant Proteins from Abcam (Lck)
    Human Recombinant Proteins from Abnova (LCK)
                  OriGene Purified Recombinant Human Protein: LCK 

    5/6 Gene Ontology (GO) cellular component terms (links to tree view) (see all 6 ):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000242 pericentriolar material IDA7513706
    GO:0005737 cytoplasm IEA--
    GO:0005794 Golgi apparatus IDA18029348
    GO:0005829 cytosol EXP2014052
    GO:0005886 plasma membrane NAS15489916
    About this table

    Antibodies for LCK: 
    Invitrogen Antibodies for LCK
    Millipore Mono- and Polyclonal Antibodies for the study of LCK
    Sigma-Aldrich Antibodies for LCK
    R&D Systems Antibodies for LCK
    Cell Signaling Technology (CST) Antibodies for LCK 
    Antibodies from Abcam (Lck), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (LCK)
    Novus Biologicals Antibodies for LCK

    Assays for LCK: 
    Invitrogen Assays for LCK
    Millipore Kits and Assays for the Analysis of LCK
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    Protein Domains/
    Families
    for LCK

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section
    5/6 InterPro domains/families (see all 6 ):
     IPR017441 Protein_kinase_ATP_BS
     IPR001245 Tyr_pkinase
     IPR000980 SH2
     IPR001452 SH3_domain
     IPR000719 Prot_kinase_core


       GeneDecks  LCK for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry P06239

    ProtoNet protein and cluster: P06239

    3 Blocks protein families:
    IPB000980 SH2 domain signature
    IPB001452 SH3 domain signature
    IPB008266 Tyrosine protein kinase


    UniProtKB/Swiss-Prot: LCK_HUMAN, P06239
    Domain: The SH2 domain mediates interaction with SQSTM1. Interaction is regulated by Ser-59
    phosphorylation
    Similarity: Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily
    Similarity: Contains 1 protein kinase domain
    Similarity: Contains 1 SH2 domain
    Similarity: Contains 1 SH3 domain

    Gene Function
    for LCK

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section
    Inhib.
    RNA:    		Invitrogen RNAi Products for gene knock-down (LCK)
    Millipore RNAi Products for the Analysis of LCK Gene knock-down
    Abnova Chimera RNAi Products for Gene knock-down (LCK)
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_005356

                  Applied Biosystems Silencer® siRNAs for LCK

                  Sigma-Aldrich siRNA and siRNA Panels for LCK  
                         Sigma-Aldrich shRNA Panels and shRNA for LCK  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for LCK
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                   OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_001042771
                                     Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_001042771
                                     untagged cDNA clones in CMV expression vector (see all 4): NM_001042771 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_001042771

    UniProtKB/Swiss-Prot: LCK_HUMAN, P06239
    Function: Tyrosine kinase that plays an essential role for the selection and maturation of
    developing T-cell in the thymus and in mature T-cell function. Is constitutively associated with
    the cytoplasmic portions of the CD4 and CD8 surface receptors and plays a key role in T-cell
    antigen receptor(TCR)-linked signal transduction pathways. Association of the TCR with a peptide
    antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I
    molecules, respectively, and thereby recruits the associated LCK to the vicinity of the TCR/CD3
    complex. LCK then phosphorylates tyrosines residues within the immunoreceptor tyrosines-based
    activation motifs (ITAMs) in the cytoplasmic tails of the TCRgamma chains and CD3 subunits,
    initiating the TCR/CD3 signaling pathway. In addition, contributes to signaling by other receptor
    molecules. Associates directly with the cytoplasmic tail of CD2, and upon engagement of the CD2
    molecule, LCK undergoes hyperphosphorylation and activation. Also plays a role in the IL2
    receptor-linked signaling pathway that controls T-cell proliferative response. Binding of IL2 to
    its receptor results in increased activity of LCK. Is expressed at all stages of thymocyte
    development and is required for the regulation of maturation events that are governed by both
    pre-TCR and mature alpha beta TCR
    Catalytic activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
    Enzyme regulation: Inhibited by tyrosine phosphorylation
    Enzyme Number (IUBMB): EC 2.7.10.2 

    Genatlas biochemistry entry for LCK:
    breakpoint in T-cell acute lymphoblastic leukemia with translocations t(1;7)(p34;q34)

    4 MGI mutant phenotypes (inferred from 3 alleles) (MGI details for Lck):

    embryogenesishematopoietic systemimmune systemreproductive system

    5/13 Gene Ontology (GO) molecular function terms (links to tree view) (see all 13 ):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000166 nucleotide binding IEA--
    GO:0001948 glycoprotein binding IPI7513706 14625311 15554700
    GO:0004715 non-membrane spanning protein tyrosine kinase activity IEA--
    GO:0004722 protein serine/threonine phosphatase activity IDA8506364
    GO:0005524 ATP binding IEA--
    About this table

    Pathways & Interactions
    for LCK

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    5/11 Invitrogen iPath™ Online BioAtlas - Pathways for LCK (see all 11 ) (Maps provided by GeneGo):
     Pleckstrin homology proteins interactions. Part VIII
     CD28 signaling
     TCR and CD28 co-stimulation in activation of NF-kB
     IL2 activation and signaling pathway
     T cell receptor signaling pathway

       GeneDecks  LCK for the pathways selected above  
    About GeneDecksing

    5/15 Millipore Pathways for LCK (see all 15 )
     Chemotaxis CXCR4 signaling pathway
     Immune response CD16 signaling in NK cells
     Immune response Role of DAP12 receptors in NK cells
     G-protein signaling M-RAS regulation pathway
     G-protein signaling TC21 regulation pathway

       GeneDecks  LCK for the pathways selected above  
    About GeneDecksing

    5/14 Sigma-Aldrich "Your Favorite Gene" Pathways for  LCK  (Your Favorite Gene powered by Ingenuity) (see all 14 ) 
     NF-&kappa
     Reelin Signaling in Neurons
     Primary Immunodeficiency Signaling
     Calcium-induced T Lymphocyte Apoptosis
     IL-2 Signaling

       GeneDecks  LCK for the pathways selected above  
    About GeneDecksing

    3 Cell Signaling Technology (CST) Pathways for LCK: 
     Translational Control: Regulation of eIF4E and p70 S6 Kinase
     NF-kB Signaling
     T Cell Receptor Signaling

       GeneDecks  LCK for the pathways selected above  
    About GeneDecksing

    3 Kegg Pathways  (Kegg details for LCK):
     hsa04650 Natural killer cell mediated cytotoxicity
     hsa04660 T cell receptor signaling pathway
     hsa05340 Primary immunodeficiency

       GeneDecks  LCK for the pathways selected above  
    About GeneDecksing
     Gene Network CentralTM Interacting Genes and Proteins Network for  LCK 


    5/246 Interacting proteins for LCK (ENSP000003282133 P062391, 2) via UniProtKB, MINT, and/or STRING (see all 246 )
    InteractantInteraction Details
    GeneCardExternal ID(s)
    MED28Q9H2041STRING (score=.941) EBI-1348, EBI-514199
    PTPN22Q9Y2R21STRING (score=.971) EBI-1348, EBI-1211241
    PTPN6P293501STRING (score=.998) EBI-1348, EBI-78260
    PTPRCP085751STRING (score=.999) EBI-1348, EBI-1341
    SH2D2AQ9NP311STRING (score=.987) EBI-1348, EBI-490630
    About this table

    5/18 Gene Ontology (GO) biological process terms (links to tree view) (see all 18 ):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006882 cellular zinc ion homeostasis IEP11739864
    GO:0006917 induction of apoptosis IMP16116473
    GO:0006919 activation of caspase activity IDA16116473
    GO:0007166 cell surface receptor linked signal transduction IEA--
    GO:0007242 intracellular signaling cascade TAS10358157
    About this table

    Drugs & Compounds
    for LCK

    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Enzo Life Sciences drugs & compounds for LCK
    Sigma-Aldrich Small Molecules for LCK:
    Antagonist Inhibitor

    Compounds for LCK available from Tocris Bioscience
    CompoundAction CAS number
    PiceatannolInhibits TNF-induced NF-kappaB activation[10083-24-6]
    About this table


    10/40 Novoseek chemical compound relationships for LCK gene (see all 40 )
    Compound   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
    tyrosine 90.84 1018 16154907 (5), 8756658 (4), 1697929 (4), 9225005 (4) (see all 99)
    phosphotyrosine 79.72 35 8618896 (2), 7539434 (2), 8626636 (2), 12922168 (2) (see all 26)
    damnacanthal 70.37 8 7547985 (3), 10050753 (1), 11182374 (1)
    a 201 58.73 2 17123354 (1)
    ara-m 57.21 1 7509083 (1)
    phosphatidylinositol 55.02 24 8845017 (3), 10636929 (1), 11694268 (1), 8096851 (1) (see all 17)
    st-pc 53.82 1 17266559 (1)
    threonine 50.03 18 7547985 (1), 8794306 (1), 10762062 (1), 8887682 (1) (see all 14)
    ionomycin 39.56 2 1717576 (1), 7536622 (1)
    phorbol-12-myristate 37.42 3 1502168 (1), 12773514 (1)
    About this table


    Transcripts
    for LCK

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section
    Inhib.
    RNA:    		Invitrogen RNAi Products for gene knock-down (LCK)
    Millipore RNAi Products for the Analysis of LCK Gene knock-down
    Abnova Chimera RNAi Products for Gene knock-down (LCK)
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_005356

                  Sigma-Aldrich siRNA and siRNA Panels for LCK  
                         Sigma-Aldrich shRNA Panels and shRNA for LCK  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_001042771  NM_005356  

    REFSEQ mRNAs for LCK gene (2 alternative transcripts): 

    NM_001042771.1   NM_005356.3   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_001042771  NM_005356  

                   OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_001042771
                                     Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_001042771
                                     untagged cDNA clones in CMV expression vector (see all 4): NM_001042771 

    Additional cDNA sequence: 

    AF228313.1 AJ865079.1 AK098027.1 AK303073.1 BC013200.1 CR594236.1 M36881.1 U07236.1 
    X04476.1 X05027.1 X06369.1 X13529.1 

    19 DOTS entries:

    DT.91672474  DT.91762501  DT.95346527  DT.121325198  DT.121325457  DT.91985416  DT.100021868  DT.414928 
    DT.121325377  DT.100749446  DT.121325427  DT.121325430  DT.75198010  DT.100739523  DT.100749445  DT.40106180 
    DT.100662923  DT.121325197  DT.97804498 

    24/114 AceView cDNA sequences (see all 114 ):

    AF228313 AA306569 AL558447 AW084769 CR594236 BI062346 BI062867 BC013200 
    AA357039 BM918144 BG686106 BM919471 BQ711028 BI063930 CB115016 BG756739 
    BI062938 AA961253 BM917288 AA354022 BF128825 BI820532 BG756534 BF129084 

    highest scoring ESTs for LCK:

    M36881 AA040695 AA040732 AA282059 AA306381 AA306569 AA309943 AA352185 AA354687 AA420981 

    Unigene Cluster for LCK:

    Lymphocyte-specific protein tyrosine kinase
    Hs.470627  [show with all ESTs]
    Unigene Representative Sequence: BC013200


    GeneLoc Exon Structure

    5/11 Alternative Splicing Database (ASD) splice patterns (SP) for LCK (see all 11 )

    ExUns: 1a · 1b · 1c · 1d · 1e ^ 2a · 2b · 2c · 2d ^ 3a · 3b ^ 4a · 4b ^ 5a · 5b · 5c ^ 6a · 6b · 6c ^ 7 ^ 8 ^ 9 ^ 10a · 10b ^ 11a · 11b ^
    SP1:                                                              -                             -                 -                                             
    SP2:                                -     -     -     -           -                             -                 -                                   -         
    SP3:                                -     -     -     -           -                             -                 -                                   -         
    SP4:                                                              -                             -                 -                 -                 -         
    SP5:                                                                                            -                 -                 -                           

    ExUns: 12 ^ 13 ^ 14a · 14b · 14c
    SP1:                              
    SP2:                              
    SP3:                              
    SP4:                              
    SP5:                              

    About this scheme

    ECgene alternative splicing isoforms for LCK

    7 Ensembl transcripts including schematic representations:
    ENST00000373562  ENST00000373564  ENST00000373557  ENST00000336890  ENST00000355928  ENST00000333070  
    ENST00000398345  

    Expression
    for LCK

    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    LCK expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for LCK

    1 / 2 / 3

    7 probe-sets matching LCK gene


    		Affymetrix
    probe-set    		 Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
    33238_at2, 3 U95-A 1 1.00 1.00 0.97 1.20 U23852 0.80 1.00 0.91 1
    2059_s_at2, 3 U95-A 1 1.00 1.00 0.98 1.31 M36881 1.00 1.00 1.00 1

    		1266_s_at2, 3 U95-A 1 1.00 1.00 0.62 0.53 M26692 0.20 1.00 0.72 1

    		204891_s_at2, 3 U133-A 1 1.00 1.00 -- -- NM_005356 0.60 1.00 0.82 1

    		204890_s_at2, 3 U133-A 1 1.00 1.00 -- -- U07236 0.60 1.00 0.82 1

    		204891_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    		204890_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    GeneDecks  LCK for binary patterns associated with the probe-sets selected above  
    About GeneDecksing
    About this table    
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: TATGTCTTGG

    SOURCE GeneReport for Unigene cluster: Hs.470627

    Expression variation in blood from EXPOLDB for LCK

    UniProtKB/Swiss-Prot: LCK_HUMAN, P06239
    Tissue specificity: Expressed specifically in lymphoid cells

    Orthologs
    for LCK

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section
    Orthologs for LCK gene from 5/7 species (see all 7 )
    Organism Gene Locus Description Human
    Similarity    		 NCBI accessions
    dog
    (Canis familiaris)    		 LCK1   -- lymphocyte-specific protein tyrosine kinase 92.99(n)
    97.45(a)    		 478151  XM_846879.1  XP_851972.1 
    cow
    (Bos taurus)    		 LCK1   -- lymphocyte-specific protein tyrosine kinase 91.94(n)
    96.27(a)    		 508890  NM_001034334.1  NP_001029506.1 
    rat
    (Rattus norvegicus)    		 Lck1   -- lymphocyte-specific protein tyrosine kinase 88.21(n)
    96.66(a)    		 313050  XM_232763.4  XP_232763.2 
    mouse
    (Mus musculus)    		 Lck1, 5 4 (59.00 cM)5
    		 lymphocyte protein tyrosine kinase1, 5 88.8(n)1
    96.86(a)1    		 168181  NM_010693.21  NP_034823.11 
     AI5734545  AK0880015  (see all 18)
    zebrafish
    (Danio rerio)    		 zgc:1366951   -- zgc:136695 67.81(n)
    71.92(a)    		 678576  NM_001040328.1  NP_001035418.1 
    About this table        Species with no ortholog for LCK

    ENSEMBL Gene Tree for LCK

    Paralogs
    for LCK

    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for LCK gene
    BLK2  LYN2  HCK2  

    SNPs/Variants
    for LCK

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/198 NCBI SNPs in LCK are shown (see all 198 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 43)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 1 posSequenceRecsAA
    Chg    		TypeMoreRecsAllele
    freq    		PopTotal
    sample    		More
    ------------
    rs118079761,2
    		A,C,F,H32524661(+) gaagcG/Agcggg 2 -- ng313Minor allele frequency- A:0.10EA WA 298
    rs109145391,2
    		C,F,H32488228(+) CCTTTG/TTTTCT 1 -- ng514Minor allele frequency- T:0.05EU EA WA 414
    rs109145451,2
    		F,H32524114(+) TGTGCA/GTGTAT 2 -- ut31 ese34Minor allele frequency- G:0.01EU EA WA 404
    rs115678411,2
    		H32514221(+) TTCCCG/AGCCTG 2 S/G mis1 ese34Minor allele frequency- A:0.00EU EA WA 376
    --
    rs109145401,2
    		C32488408(+) CGCATA/GTGCAC 1 -- ng510--------
    --
    rs6903751,2
    		A32487742(-) tactcA/Gggagg 1 -- ng510--------
    rs133129961,2
    		H32489471(+) GCTACG/AACGGC 1 -- ut514Minor allele frequency- A:0.00EU EA WA 418
    rs6951611,2
    		A,C,F,H,O32516453(-) TTACAT/CTCCCA 2 -- int115Minor allele frequency- C:0.25EU EA WA NA 1396
    rs10044201,2
    		A,C,F,H32502289(+) CACAGG/TGCTCA 1 -- int112Minor allele frequency- N:0.01EU EA WA NA 1032
    rs64257951,2
    		A,C,F,H32490686(+) TCAGCT/AGGTCC 1 -- int14Minor allele frequency- A:0.18EU EA WA 418
    About this table

    HapMap Linkage Disequilibrium images for LCK (up to first 250kb)

    Disorders & Mutations
    for LCK

    (in which this Gene is Involved, According to OMIM, UniProtKB, Novoseek, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    		 OMIM: 153390

    UniProtKB/Swiss-Prot: LCK_HUMAN, P06239

  • A chromosomal aberration involving LCK is found in leukemias. Translocation
    t(1;7)(p34;q34) with TCRB

    • 10/49 Novoseek disease relationships for LCK gene (see all 49 )

    Disease   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
    leukemia t-cell 65.84 25 2116390 (2), 8139546 (2), 9032329 (2), 2384277 (2) (see all 13)
    immunodeficiency 35.54 16 1535787 (2), 11351273 (1), 15976924 (1), 8794306 (1) (see all 13)
    thymoma 31.39 4 7997241 (1), 14688799 (1), 15634634 (1)
    t-all 24.91 3 1663780 (1), 1680958 (1), 12542476 (1)
    lymphoma t-cell 24.07 2 2285146 (1), 15513966 (1)
    hematopoietic malignancies 18.19 3 8712692 (1), 8086341 (1)
    autoimmune diseases 14.34 5 19267483 (1), 11717807 (1), 17082661 (1), 16673863 (1)
    lymphopenia 5.11 7 11351273 (3), 12614355 (1)
    colon cancer 4.97 1 8869106 (1)
    lymphoma non-hodgkins 4.95 1 7636203 (1)
    About this table

    1 PharmGKB disease relationship for LCK gene
    Disease PharmGKB Relations PubMed IDs for articles supporting these relationships
    Leukemia, Lymphocytic, Acute, L1FA  GN  12086872
    About this table

    Genetic Association Database: LCK
    Tumor Gene Database: LCK

    Medical News
    for LCK

    (Possibly Related Articles in Doctor's Guide)
    About This Section

    Publications
    for LCK

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    		10/894 PubMed articles for LCK gene (see all 894 ):

    Search for LCK

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  
      Query String
    		PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    Genome Databases showing LCK

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section
    Entrez Gene: 3932 HGNC: 6524 AceView: LCK Ensembl:ENSG00000182866 euGenes: HUgn3932
    ECgene: LCK H-InvDB: LCK

    Other Databases showing LCK

    (According to HUGE)
    About This Section

    		   --

    Specialized Databases showing LCK

    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    ATLAS Chromosomes in Cancer entry for LCK Genetics and Cytogenetics in Oncology and Haematology
    Wikipedia http://en.wikipedia.org/wiki/Lck

    Licensable Technologies
    for LCK

    (Available from WIS Yeda, Salk, Tufts)
    About This Section

    		  --

    Services
    for LCK

    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for LCK:
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     Tagged/untagged cDNA clones
     Validated SYBR primer pairs
     Purified proteins from HEK293T cells
     Western blot validated antibodies
     shRNA in GFP-retroviral vector
     Tocris compounds for LCK




     Drugs/Proteins for LCK
     Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
     Antibodies for LCK

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