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LASS6 Gene

protein-coding   GIFtS: 47

GC02P169021
LAG1 homolog, ceramide synthase 6
(Previous name: LAG1 longevity assurance homolog 6 (S. cerevisiae) )
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
CerS6 1, 2
MGC129949 2
MGC129950 2
Descriptions
LAG1 homolog, ceramide synthase 6 2
LAG1 longevity assurance homolog 6 2
LAG1 longevity assurance homolog 6 (S. cerevisiae) 1
longevity assurance homolog 6 2
External Ids
HGNC: 238261
Entrez Gene: 2537822
UniProtKB: Q6ZMG93
Ensembl: ENSG000001722927
Search outside databases for aliases for LASS6 gene

Previous GC identifers: GC02P169515 GC02P169138

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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UniProtKB/Swiss-Prot: LASS6_HUMAN, Q6ZMG9
Function: May be involved in sphingolipid synthesis or its regulation (By similarity)

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
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Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the LASS6 gene  

Entrez Gene cytogenetic band: 2q24.3   Ensembl cytogenetic band:  2q24.3   HGNC cytogenetic band: 2q31

LASS6 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 2         GeneLoc Exon Structure

GeneLoc location for GC02P169021:     (about GC identifiers)

Start:
169,021,005 bp from pter
End:
169,339,398 bp from pter
Size:
318,394 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000002.10  NT_005403.16  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: LASS6_HUMAN, Q6ZMG9 (See protein sequence)
Recommended Name: LAG1 longevity assurance homolog 6  
Size: 384 amino acids; 44890 Da
Subcellular location: Nucleus membrane; Multi-pass membrane protein (Potential). Endoplasmic
reticulum membrane; Multi-pass membrane protein (By similarity)
Sequence caution: Sequence=AAH30800.2; Type=Erroneous translation; Note=Wrong choice of frame;
Secondary accessions: Q8N617

Post-translational modifications:

  • Glycosylation on Asn-18 is not necessary for function (By similarity)1


  • REFSEQ proteins: NP_982288.1  

    ENSEMBL proteins: 
    ENSP00000376453 ENSP00000306579 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (LASS6)
    Human Recombinant Proteins from Abnova (LASS6)
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    5/6 Gene Ontology (GO) cellular component terms (links to tree view) (see all 6 ):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634 nucleus IEA--
    GO:0005783 endoplasmic reticulum IEA--
    GO:0005789 endoplasmic reticulum membrane IEA--
    GO:0016020 membrane IEA--
    GO:0016021 integral to membrane IEA--
    About this table

    Antibodies for LASS6: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Sigma-Aldrich Antibodies for LASS6
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (LASS6), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (LASS6)
    Novus Biologicals Antibodies for LASS6

    Assays for LASS6: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    4 InterPro domains/families:
     IPR016439 Longevity_assurance_LAG1_LAC1
     IPR001356 Homeobox
     IPR017970 Homeobox_CS
     IPR006634 TLC


       GeneDecks  LASS6 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q6ZMG9

    ProtoNet protein and cluster: Q6ZMG9

    2 Blocks protein families:
    IPB005547 Longevity-assurance protein (LAG1)
    IPB006634 TRAM


    UniProtKB/Swiss-Prot: LASS6_HUMAN, Q6ZMG9
    Similarity: Contains 1 homeobox DNA-binding domain
    Similarity: Contains 1 TLC (TRAM/LAG1/CLN8) domain

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (LASS6)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (LASS6)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_203463

                  Applied Biosystems Silencer® siRNAs for LASS6

                  Sigma-Aldrich siRNA for LASS6  
                         Sigma-Aldrich shRNA for LASS6  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for LASS6
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_203463
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_203463
                                     untagged cDNA clone in CMV expression vector: NM_203463 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_203463

    UniProtKB/Swiss-Prot: LASS6_HUMAN, Q6ZMG9
    Function: May be involved in sphingolipid synthesis or its regulation (By similarity)

    1 MGI phenotypic allele for Lass6 (no phenotypes)


    3 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003700 transcription factor activity IEA--
    GO:0043565 sequence-specific DNA binding IEA--
    GO:0050291 sphingosine N-acyltransferase activity IEA--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


    1 Sigma-Aldrich "Your Favorite Gene" Pathway for  LASS6  (Your Favorite Gene powered by Ingenuity) 
     Sphingolipid Metabolism

       GeneDecks  LASS6 for the pathways selected above  
    About GeneDecksing

      --

    4 Gene Ontology (GO) biological process terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006355 regulation of transcription, DNA-dependent IEA--
    GO:0008610 lipid biosynthetic process IEA--
    GO:0030148 sphingolipid biosynthetic process IEA--
    GO:0046513 ceramide biosynthetic process IEA--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for LASS6

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (LASS6)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (LASS6)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_203463

                  Sigma-Aldrich siRNA for LASS6  
                         Sigma-Aldrich shRNA for LASS6  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_203463  

    REFSEQ mRNAs for LASS6 gene: 

    NM_203463.1   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_203463  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_203463
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_203463
                                     untagged cDNA clone in CMV expression vector: NM_203463 

    Additional cDNA sequence: 

    AK023042.1 AK024891.1 AK091391.1 AK172775.1 AL080192.1 BC030800.2 BC109284.1 BC109285.1 

    9 DOTS entries:

    DT.440675  DT.100027737  DT.91754671  DT.100786463  DT.121003426  DT.95254207  DT.121003456  DT.92434231 
    DT.100702022 

    14 AceView cDNA sequences:

    AI150099 BC035245 BI036722 F00280 BQ130079 BG722671 AA394072 BU853076 
    BF921625 AA838493 AA292363 AW994194 T07901 BF800177 

    highest scoring ESTs for LASS6:

    AI360291 AI936475 AL080192 N24987 AA036719 AA037829 AA037830 AA057358 AA090687 AA167065 

    Unigene Clusters for LASS6:

    LAG1 homolog, ceramide synthase 6
    Hs.506829  [show with all ESTs], Hs.718403  [show with all ESTs]
    Unigene Representative Sequences: BC035245, NM_203463


    GeneLoc Exon Structure

    2 Ensembl transcripts including schematic representations:
    ENST00000392687  ENST00000305747  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    LASS6 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for LASS6

    1 / 2 / 3

    12 probe-sets matching LASS6 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

    52653_at2, 3 U95-B 1 1.00 1.00 0.83 1.20 AI360291 1.00 1.00 1.00 1

    47434_at2, 3 U95-B 1 1.00 1.00 0.78 1.08 N24987 1.00 1.00 1.00 1
    75411_at2, 3 U95-E 1 1.00 1.00 0.78 0.89 AI936475 1.00 1.00 1.00 1
    38630_at2, 3 U95-A 1 0.88 1.00 0.77 0.84 AL080192 1.00 1.00 1.00 1

    242019_at2, 3 U133-B 1 1.00 1.00 -- -- BG257755 0.80 1.00 0.91 1

    235463_s_at2, 3 U133-B 1 1.00 1.00 -- -- AI081356 0.80 1.00 0.91 1

    212442_s_at2, 3 U133-A 1 1.00 1.00 -- -- BG289001 0.80 1.00 0.91 1

    212446_s_at2, 3 U133-A 1 1.00 1.00 -- -- AI658534 0.80 1.00 0.91 1

    242019_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    235463_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    212442_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    212446_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    GeneDecks  LASS6 for binary patterns associated with the probe-sets selected above  
    About GeneDecksing
    About this table    
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: --

    SOURCE GeneReport for Unigene clusters: Hs.506829 Hs.718403

    Expression variation in blood from EXPOLDB for LASS6

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for LASS6 gene from 5/9 species (see all 9 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    LASS61   -- LAG1 homolog, ceramide synthase 6 93.8(n)
    95.81(a)
    488387  XM_545509.2  XP_545509.2 
    chimpanzee
    (Pan troglodytes)
    LASS61   -- LAG1 homolog, ceramide synthase 6 99.74(n)
    100(a)
    741293  XM_001154846.1  XP_001154846.1 
    cow
    (Bos taurus)
    LASS61   -- LAG1 homolog, ceramide synthase 6 93.14(n)
    93.49(a)
    616901  XM_869038.2  XP_874131.2 
    rat
    (Rattus norvegicus)
    Lass61   -- LAG1 homolog, ceramide synthase 6 90.67(n)
    96.43(a)
    366065  XM_345363.3  XP_345364.3 
    mouse
    (Mus musculus)
    Lass61, 5 25
    LAG1 homolog, ceramide synthase 61, 5 90.28(n)1
    95.31(a)1
    2414471  NM_172856.21  NP_766444.11 
     AI8518655  AK0288495  (see all 17)
    About this table        Species with no ortholog for LASS6

    ENSEMBL Gene Tree for LASS6
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for LASS6 gene
    LASS52  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/1737 NCBI SNPs in LASS6 are shown (see all 1737 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 514)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 2 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs10637291,2
    C,F,H,O169338729(+) TTTTCA/GTGGAG 1 -- ut317Minor allele frequency- G:0.37EU EA WA NA 538
    rs83041,2
    A,C,F,H169339081(+) GGTGTT/GGACAT 1 -- ut31 ese320Minor allele frequency- G:0.14MN NA EA NS EU WA 2702
    rs130015931,2
    C,F,H169337401(+) CTAAGG/ACCAGC 1 -- ut31 ese313Minor allele frequency- A:0.01NA EU EA WA 1160
    rs171989171,2
    C,F,H169338550(+) CACTGG/ACCACA 1 -- ut31 ese311Minor allele frequency- A:0.01NA EU EA WA 978
    rs38143781,2
    A,C169338738(-) TACAAG/TTTTCT 1 -- ut310--------
    rs23033881,2
    F,H169336565(-) ATGCAA/GTTCTT 1 -- ut315Minor allele frequency- G:0.01EA EU WA 1906
    rs168558191,2
    F,H169335517(+) GAGAAA/GGACTA 1 -- ut31 trp37Minor allele frequency- G:0.07NA EU EA WA 556
    rs73554721,2
    C,H169334753(+) GTGAAG/AGAGAT 1 -- ut314Minor allele frequency- A:0.00EU EA WA 418
    --
    rs133908921,2
    C,F169338925(+) GTCTCT/CGCAGC 1 -- ut31 ese33Minor allele frequency- C:0.08NA 142
    --
    rs64330661,2
    A,C169019884(+) TCTCtC/Gtgtgt 1 -- ng51 trp30--------
    About this table

    HapMap Linkage Disequilibrium images for LASS6 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

      --

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    8 PubMed articles for LASS6 gene:
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 253782 HGNC: 23826 AceView: LASS6.1 Ensembl:ENSG00000172292 euGenes: HUgn253782
    ECgene: LASS6 H-InvDB: LASS6
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for LASS6:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



      Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
      Invitrogen Antibodies  Invitrogen Assays
      Invitrogen Clones  Invitrogen Q-PCR Products
      Invitrogen Human Recombinant Kinases  Invitrogen Custom Antibody and Peptide Service
      Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for LASS6-related products

     Millipore Custom Antibody & Bulk Services
     Millipore Preclinical / Clinical Development Services
     Millipore Immunoassay Services
     Millipore Target Screening & Profiling Services


     Predesigned and custom siRNAs for LASS6 Antibodies for LASS6
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for LASS6 Browse small molecules at Sigma-Aldrich
     "Your Favorite Gene" Pathwaysfeedback


      
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     Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
     Antibodies for LASS6

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