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LASS5 Gene

protein-coding   GIFtS: 49

GC12M048809
LAG1 homolog, ceramide synthase 5
(Previous name: LAG1 longevity assurance homolog 5 (S. cerevisiae) )
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases
CerS5 1, 2
FLJ25304 1, 2
MGC45411 1, 2
Trh4 1, 2
Descriptions
LAG1 homolog, ceramide synthase 5 2
LAG1 longevity assurance homolog 5 2
LAG1 longevity assurance homolog 5 (S. cerevisiae) 1
External Ids
HGNC: 237491
Entrez Gene: 910122
UniProtKB: Q8N5B73
Ensembl: ENSG000001396247
Search outside databases for aliases for LASS5 gene

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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UniProtKB/Swiss-Prot: LASS5_HUMAN, Q8N5B7
Function: May be either a bona fide (dihydro)ceramide synthase or a modulator of its activity. When
overexpressed in cells is involved in the production of sphingolipids containing mainly one fatty
acid donnor (N-linked palmitoyl- (C16) ceramide) in a fumonisin B1-independent manner (By
similarity)

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
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Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the LASS5 gene  

Entrez Gene cytogenetic band: 12q13.13   Ensembl cytogenetic band:  12q13.13   HGNC cytogenetic band: 12q13.12

LASS5 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 12         GeneLoc Exon Structure

GeneLoc location for GC12M048809:     (about GC identifiers)

Start:
48,809,848 bp from pter
End:
48,847,412 bp from pter
Size:
37,565 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000012.10  NT_029419.11  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: LASS5_HUMAN, Q8N5B7 (See protein sequence)
Recommended Name: LAG1 longevity assurance homolog 5  
Size: 392 amino acids; 45752 Da
Subcellular location: Nucleus membrane; Multi-pass membrane protein (Potential). Endoplasmic
reticulum membrane; Multi-pass membrane protein (By similarity)

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_671723.1  

    ENSEMBL proteins: 
    ENSP00000325485 ENSP00000369536 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (Lass5)
    Human Recombinant Proteins from Abnova (LASS5)
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    5/7 Gene Ontology (GO) cellular component terms (links to tree view) (see all 7 ):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634 nucleus IEA--
    GO:0005783 endoplasmic reticulum IEA--
    GO:0005789 endoplasmic reticulum membrane IEA--
    GO:0005792 microsome IDA16951403
    GO:0016020 membrane IEA--
    About this table

    Antibodies for LASS5: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Sigma-Aldrich Antibodies for LASS5
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (Lass5), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (LASS5)
    Search Novus for antibodies for LASS5

    Assays for LASS5: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    4 InterPro domains/families:
     IPR016439 Longevity_assurance_LAG1_LAC1
     IPR001356 Homeobox
     IPR017970 Homeobox_CS
     IPR006634 TLC


       GeneDecks  LASS5 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q8N5B7

    ProtoNet protein and cluster: Q8N5B7

    2 Blocks protein families:
    IPB005547 Longevity-assurance protein (LAG1)
    IPB006634 TRAM


    UniProtKB/Swiss-Prot: LASS5_HUMAN, Q8N5B7
    Similarity: Contains 1 homeobox DNA-binding domain
    Similarity: Contains 1 TLC (TRAM/LAG1/CLN8) domain

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (LASS5)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (LASS5)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_147190

                  Applied Biosystems Silencer® siRNAs for LASS5

                  Sigma-Aldrich siRNA for LASS5  
                         Sigma-Aldrich shRNA for LASS5  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Browse Clone Ranger at Invitrogen for clones
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_147190
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_147190
                                     untagged cDNA clone in CMV expression vector: NM_147190 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_147190

    UniProtKB/Swiss-Prot: LASS5_HUMAN, Q8N5B7
    Function: May be either a bona fide (dihydro)ceramide synthase or a modulator of its activity. When
    overexpressed in cells is involved in the production of sphingolipids containing mainly one fatty
    acid donnor (N-linked palmitoyl- (C16) ceramide) in a fumonisin B1-independent manner (By
    similarity)

    3 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003700 transcription factor activity IEA--
    GO:0043565 sequence-specific DNA binding IEA--
    GO:0050291 sphingosine N-acyltransferase activity IDA16951403
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


    1 Sigma-Aldrich "Your Favorite Gene" Pathway for  LASS5  (Your Favorite Gene powered by Ingenuity) 
     Sphingolipid Metabolism

       GeneDecks  LASS5 for the pathways selected above  
    About GeneDecksing

      --

    3 Gene Ontology (GO) biological process terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006355 regulation of transcription, DNA-dependent IEA--
    GO:0008610 lipid biosynthetic process IEA--
    GO:0046513 ceramide biosynthetic process IDA16951403
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for LASS5

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (LASS5)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (LASS5)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_147190

                  Sigma-Aldrich siRNA for LASS5  
                         Sigma-Aldrich shRNA for LASS5  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_147190  

    REFSEQ mRNAs for LASS5 gene: 

    NM_147190.2   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_147190  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_147190
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_147190
                                     untagged cDNA clone in CMV expression vector: NM_147190 

    Additional cDNA sequence: 

    AK026014.1 AK027087.1 AK055740.1 AK058033.1 AK294521.1 AK298924.1 AK300937.1 AK308930.1 
    BC014637.1 BC032565.1 BC033558.1 CR602245.1 U15426.1 

    20 DOTS entries:

    DT.100805869  DT.100025003  DT.70104773  DT.444776  DT.92436993  DT.97816602  DT.428724  DT.95098210 
    DT.91675317  DT.121183231  DT.91952126  DT.86838936  DT.97796847  DT.97845422  DT.100805870  DT.121183195 
    DT.75197877  DT.92436989  DT.92436992  DT.121183191 

    24/243 AceView cDNA sequences (see all 243 ):

    BI870365 CB137489 AA056080 BM798014 BC014637 AA018127 BM665614 AA489165 
    CA439004 AI151163 AA236771 BC033558 BM711803 CR602245 BX118634 BX113560 
    Z42984 CB216425 AA053726 BQ721513 AA417054 AI917554 BX401323 BQ017508 

    highest scoring ESTs for LASS5:

    AI830401 AK026014 AL710677 BC014637 BC032565 BC033558 BE296562 BE742669 BF304449 BF306032 

    Unigene Cluster for LASS5:

    LAG1 homolog, ceramide synthase 5
    Hs.270525  [show with all ESTs]
    Unigene Representative Sequence: BC033558


    GeneLoc Exon Structure

    5/21 Alternative Splicing Database (ASD) splice patterns (SP) for LASS5 (see all 21 )

    ExUns: 1a · 1b · 1c · 1d · 1e · 1f ^ 2 ^ 3 ^ 4 ^ 5 ^ 6 ^ 7a · 7b · 7c ^ 8 ^ 9a · 9b · 9c · 9d · 9e · 9f ^ 10a · 10b ^ 11 ^ 12a · 12b ·
    SP1:                                      -     -     -     -     -     -     -     -           -     -                                                         
    SP2:                                                                                                                                                            
    SP3:                                      -     -     -     -           -     -     -           -     -                                                         
    SP4:                                      -     -     -     -     -     -     -     -           -     -                                               -     -   
    SP5:                                      -     -     -     -     -     -     -     -           -     -                             -     -                     

    ExUns: 12c · 12d ^ 13a · 13b ^ 14a · 14b ^ 15a · 15b ^ 16a · 16b · 16c · 16d ^ 17 ^ 18 ^ 19a · 19b · 19c
    SP1:                                      -     -     -                 -     -     -                     
    SP2:                                      -     -     -                       -     -                     
    SP3:                                                                                                      
    SP4:  -     -                                                                                             
    SP5:                                                                                                      

    About this scheme

    ECgene alternative splicing isoforms for LASS5

    2 Ensembl transcripts including schematic representations:
    ENST00000317551  ENST00000380189  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    LASS5 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for LASS5

    1 / 2 / 3

    6 probe-sets matching LASS5 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
    49885_at2, 3 U95-B 1 1.00 1.00 0.97 0.66 AA213828 0.60 1.00 0.82 1

    53053_at2, 3 U95-B 2 0.62 0.95 0.31 1.43 AI830401 0.80 1.00 0.91 1

    224951_at2, 3 U133-B 1 1.00 1.00 -- -- BE348305 0.40 0.67 0.55 1

    239491_at2, 3 U133-B 1 1.00 1.00 -- -- AI089312 0.40 1.00 0.76 1

    224951_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    239491_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    GeneDecks  LASS5 for binary patterns associated with the probe-sets selected above  
    About GeneDecksing
    About this table    
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: GGAAGAGGGT

    SOURCE GeneReport for Unigene cluster: Hs.270525
    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for LASS5 gene from 5/14 species (see all 14 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    LASS51   -- LAG1 homolog, ceramide synthase 5 91.16(n)
    89.54(a)
    477611  XM_534805.2  XP_534805.2 
    cow
    (Bos taurus)
    LASS51   -- LAG1 homolog, ceramide synthase 5 91.58(n)
    90.31(a)
    530776  XM_882294.2  XP_887387.2 
    rat
    (Rattus norvegicus)
    Lass51   -- LAG1 homolog, ceramide synthase 5 82.82(n)
    79.23(a)
    366984  XM_345870.3  XP_345871.3 
    mouse
    (Mus musculus)
    Lass51, 5 155
    LAG1 homolog, ceramide synthase 51, 5 82.57(n)1
    76.53(a)1
    719491  NM_028015.21  NP_082291.11 
     AK0102415  AK0756945  (see all 21)
    chicken
    (Gallus gallus)
    LASS51   -- LAG1 homolog, ceramide synthase 5 74.89(n)
    78.15(a)
    426879  XM_424486.2  XP_424486.2 
    About this table        Species with no ortholog for LASS5

    ENSEMBL Gene Tree for LASS5
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for LASS5 gene
    LASS62  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/314 NCBI SNPs in LASS5 are shown (see all 314 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 57)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 12 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs15548451,2
    A,C,F,H,O48809771(+) TCAGAC/TCTGGA 1 -- ng5112Minor allele frequency- T:0.31MN NA EU EA WA 1148
    rs72791,2
    A,C,F,H48810214(-) CTCCTC/TCTTTT 1 -- ut31 trp39Minor allele frequency- T:0.28MN NA EU EA WA 926
    rs73029811,2
    A,C,F,H48824082(+) AATACG/AGAGTG 1 R/C mis1 ese314Minor allele frequency- A:0.19EU EA WA NA 1236
    rs75321,2
    A,C,F,H48810231(-) ATGCCG/AACTCC 1 -- ut31 ese39Minor allele frequency- A:0.26MN NA EU EA WA 930
    rs123024651,2
    C,F,H48809796(+) GCTTAT/CGGATT 1 -- ng514Minor allele frequency- C:0.02EU EA WA 416
    rs107833411,2
    A,F,H48849247(+) AAAGCT/AGCCTC 1 -- ng314Minor allele frequency- A:0.43EU EA WA 418
    rs22935561,2
    C,F,H48810131(+) GGAACA/GGTACA 1 -- ut318Minor allele frequency- G:0.06EA NA EU WA 2042
    --
    rs111692941,2
    A48848265(+) GCCGAA/GATCGC 1 -- ng310--------
    rs123180051,2
    H48847669(+) CTGCTG/AGTCTA 1 -- ng314Minor allele frequency- A:0.00EU EA WA 420
    rs123177931,2
    H48847479(+) CACTGC/ACACGG 1 -- ng314Minor allele frequency- A:0.00EU EA WA 420
    About this table

    HapMap Linkage Disequilibrium images for LASS5 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

      --

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    9 PubMed articles for LASS5 gene:
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 91012 HGNC: 23749 AceView: LASS5 Ensembl:ENSG00000139624 euGenes: HUgn91012
    ECgene: LASS5 H-InvDB: LASS5
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for LASS5:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



      Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
      Invitrogen Antibodies  Invitrogen Assays
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      Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for LASS5-related products

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     Predesigned and custom siRNAs for LASS5 Antibodies for LASS5
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for LASS5 Browse small molecules at Sigma-Aldrich
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     Search Novus for antibodies for LASS5

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