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LASS4 Gene

protein-coding   GIFtS: 54

GC19P008180
LAG1 homolog, ceramide synthase 4
(Previous name: LAG1 longevity assurance homolog 4 (S. cerevisiae) )
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
CerS4 1, 2
FLJ12089 1, 2
Trh1 1, 2
Descriptions
LAG1 homolog, ceramide synthase 4 2
LAG1 longevity assurance homolog 4 2
LAG1 longevity assurance homolog 4 (S. cerevisiae) 1
External Ids
HGNC: 237471
Entrez Gene: 796032
UniProtKB: Q9HA823
Ensembl: ENSG000000906617
Search outside databases for aliases for LASS4 gene

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: LASS4_HUMAN, Q9HA82
Function: May be either a bona fide (dihydro)ceramide synthase or a modulator of its activity. When
overexpressed in cells is involved in the production of sphingolipids containing different fatty
acid donnors (N-linked stearoyl- (C18) or arachidoyl- (C20) ceramides) in a fumonisin
B1-independent manner (By similarity)

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the LASS4 gene  

Entrez Gene cytogenetic band: 19p13.2   Ensembl cytogenetic band:  19p13.2   HGNC cytogenetic band: 19p13.3

LASS4 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 19         GeneLoc Exon Structure

GeneLoc location for GC19P008180:     (about GC identifiers)

Start:
8,180,257 bp from pter
End:
8,233,302 bp from pter
Size:
53,046 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000019.8  NT_077812.2  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: LASS4_HUMAN, Q9HA82 (See protein sequence)
Recommended Name: LAG1 longevity assurance homolog 4  
Size: 394 amino acids; 46399 Da
Subcellular location: Nucleus membrane; Multi-pass membrane protein (Potential). Endoplasmic
reticulum membrane; Multi-pass membrane protein (By similarity)

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_078828.2  

    ENSEMBL proteins: 
    ENSP00000251363 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (LASS4)
    Human Recombinant Proteins from Abnova (LASS4)
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    5/6 Gene Ontology (GO) cellular component terms (links to tree view) (see all 6 ):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634 nucleus IEA--
    GO:0005783 endoplasmic reticulum IEA--
    GO:0005789 endoplasmic reticulum membrane IEA--
    GO:0016020 membrane IEA--
    GO:0016021 integral to membrane IEA--
    About this table

    Antibodies for LASS4: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Sigma-Aldrich Antibodies for LASS4
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (LASS4), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (LASS4)
    Novus Biologicals Antibodies for LASS4

    Assays for LASS4: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    4 InterPro domains/families:
     IPR016439 Longevity_assurance_LAG1_LAC1
     IPR001356 Homeobox
     IPR017970 Homeobox_CS
     IPR006634 TLC


       GeneDecks  LASS4 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q9HA82

    ProtoNet protein and cluster: Q9HA82

    2 Blocks protein families:
    IPB005547 Longevity-assurance protein (LAG1)
    IPB006634 TRAM


    UniProtKB/Swiss-Prot: LASS4_HUMAN, Q9HA82
    Similarity: Contains 1 homeobox DNA-binding domain
    Similarity: Contains 1 TLC (TRAM/LAG1/CLN8) domain

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (LASS4)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (LASS4)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_024552

                  Applied Biosystems Silencer® siRNAs for LASS4

                  Sigma-Aldrich siRNA for LASS4  
                         Sigma-Aldrich shRNA for LASS4  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for LASS4
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_024552
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_024552
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_024552 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_024552

    UniProtKB/Swiss-Prot: LASS4_HUMAN, Q9HA82
    Function: May be either a bona fide (dihydro)ceramide synthase or a modulator of its activity. When
    overexpressed in cells is involved in the production of sphingolipids containing different fatty
    acid donnors (N-linked stearoyl- (C18) or arachidoyl- (C20) ceramides) in a fumonisin
    B1-independent manner (By similarity)

    3 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003700 transcription factor activity IEA--
    GO:0043565 sequence-specific DNA binding IEA--
    GO:0050291 sphingosine N-acyltransferase activity IEA--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


    1 Sigma-Aldrich "Your Favorite Gene" Pathway for  LASS4  (Your Favorite Gene powered by Ingenuity) 
     Sphingolipid Metabolism

       GeneDecks  LASS4 for the pathways selected above  
    About GeneDecksing


    3 Interacting proteins for LASS4 (ENSP000002513633 Q9HA822) via UniProtKB, MINT, and/or STRING
    InteractantInteraction Details
    GeneCardExternal ID(s)
    BMP2ENSP000003681043STRING (score=.869)
    TRHENSP000003034523STRING (score=.722)
    --Q1HVH92MINT-6769802
    About this table

    4 Gene Ontology (GO) biological process terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006355 regulation of transcription, DNA-dependent IEA--
    GO:0008610 lipid biosynthetic process IEA--
    GO:0030148 sphingolipid biosynthetic process IEA--
    GO:0046513 ceramide biosynthetic process IEA--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for LASS4

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (LASS4)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (LASS4)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_024552

                  Sigma-Aldrich siRNA for LASS4  
                         Sigma-Aldrich shRNA for LASS4  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_024552  

    REFSEQ mRNAs for LASS4 gene: 

    NM_024552.2   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_024552  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_024552
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_024552
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_024552 

    Additional cDNA sequence: 

    AK022151.1 AK055414.1 AK222621.1 AK225394.1 BC009828.2 BC063621.1 CR598338.1 CR614629.1 
    CR623019.1 

    19 DOTS entries:

    DT.75103241  DT.91741576  DT.97773877  DT.95113227  DT.113394  DT.100019238  DT.97782292  DT.100829415 
    DT.95113232  DT.100811526  DT.95336878  DT.100749538  DT.86856887  DT.91741577  DT.91873859  DT.97803812 
    DT.121456286  DT.91659967  DT.99959718 

    24/184 AceView cDNA sequences (see all 184 ):

    BQ214330 AW135503 BM697971 BE893168 BQ059431 BI490050 BM909942 CR614629 
    BU149864 BM045289 BU838297 BM927305 BQ684050 BU674824 BM667138 CR623019 
    BF724636 AW440633 BG695862 BI822463 CR598338 BI754920 AW135505 BM925905 

    highest scoring ESTs for LASS4:

    T68813 AA025778 AA025779 AA025920 AA282891 AA677312 AA700750 AA703705 AA988187 AI055945 

    Unigene Cluster for LASS4:

    LAG1 homolog, ceramide synthase 4
    Hs.515111  [show with all ESTs]
    Unigene Representative Sequence: AK055414


    GeneLoc Exon Structure

    5/7 Alternative Splicing Database (ASD) splice patterns (SP) for LASS4 (see all 7 )

    ExUns: 1 ^ 2a · 2b · 2c · 2d ^ 3 ^ 4a · 4b ^ 5 ^ 6 ^ 7a · 7b · 7c · 7d ^ 8a · 8b ^ 9a · 9b ^ 10 ^ 11 ^ 12 ^ 13 ^ 14 ^ 15 ^ 16
    SP1:                                -     -                       -     -     -     -                                                                     
    SP2:                                -     -           -     -     -     -                                                                                 
    SP3:                                                                                                                                                      
    SP4:                                -                 -     -     -     -                                                                                 
    SP5:                                -     -           -     -     -                                                                                       

    About this scheme

    ECgene alternative splicing isoforms for LASS4

    1 Ensembl transcript including schematic representation:
    ENST00000251363  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    LASS4 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for LASS4

    1 / 2 / 3

    3 probe-sets matching LASS4 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
    52955_at2, 3 U95-B 1 0.81 1.00 1.00 1.00 T68813 1.00 1.00 1.00 1

    218922_s_at2, 3 U133-A 1 1.00 1.00 -- -- NM_024552 0.60 1.00 0.82 1

    218922_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    GeneDecks  LASS4 for binary patterns associated with the probe-sets selected above  
    About GeneDecksing
    About this table    
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: AAGAAGGGCC

    SOURCE GeneReport for Unigene cluster: Hs.515111
    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for LASS4 gene from 5/8 species (see all 8 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    LASS41   -- LAG1 homolog, ceramide synthase 4 80.83(n)
    75.83(a)
    485008  XM_542126.2  XP_542126.2 
    chimpanzee
    (Pan troglodytes)
    LASS41   -- LAG1 homolog, ceramide synthase 4 98.9(n)
    98.22(a)
    456435  XM_001157188.1  XP_001157188.1 
    cow
    (Bos taurus)
    LASS41   -- LAG1 homolog, ceramide synthase 4 78.63(n)
    70.99(a)
    505233  NM_001015520.1  NP_001015520.1 
    rat
    (Rattus norvegicus)
    Lass41   -- LAG1 homolog, ceramide synthase 4 77.27(n)
    69.72(a)
    304208  XM_221796.4  XP_221796.4 
    mouse
    (Mus musculus)
    Lass41, 5 85
    LAG1 homolog, ceramide synthase 41, 5 76.08(n)1
    68.96(a)1
    672601  NM_026058.31  NP_080334.21 
     AA9307785  AI5518085  (see all 30)
    About this table        Species with no ortholog for LASS4

    ENSEMBL Gene Tree for LASS4
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for LASS4 gene
    LASS32  LASS22  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/384 NCBI SNPs in LASS4 are shown (see all 384 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 91)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 19 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs362591,2
    A,C,F,H,O8232904(+) ACGGGA/GCAGCT 1 A/T mis1 ese315Minor allele frequency- N:0.00MN EA NA EU WA 2697
    rs128911,2
    A,C,F,H8233196(+) TCTGGG/CAGCCA 1 -- ut318Minor allele frequency- C:0.16MN NA EU EA WA 744
    rs281331,2
    A,C,F,H8233502(+) GCCCCC/TGACTC 1 -- ng3112Minor allele frequency- T:0.12NA EA EU WA 2586
    rs171593881,2
    C,F,H8226555(+) GTTCCG/AGAGAC 1 Q/R mis1 ese311Minor allele frequency- A:0.21NA EU EA WA 962
    --
    rs362601,2
    A,C,F8233161(+) CACCCG/ACCCTT 1 -- ut31 ese31Minor allele frequency- A:0.32MN 184
    rs171603481,2
    C,F,H8232866(+) GGAGGC/TGGCGG 1 A/V mis113Minor allele frequency- N:0.01NA EA EU WA 1128
    rs171603491,2
    C,F,H8232944(+) CCCTCG/AGAGCC 1 Q/R mis112Minor allele frequency- A:0.12NA EU EA WA 1048
    rs9224421,2
    C,F8180254(+) CGGCCA/GGGAGC 1 -- ut511Minor allele frequency- G:0.33MN 184
    rs362611,2
    A,C8233244(+) GCTGTA/GGCCTG 1 -- ut31 ese30--------
    rs10445471,2
    F,H8232999(+) CCGGGC/TGGGGC 1 -- ut31 ese35Minor allele frequency- T:0.14MN EU EA WA 602
    About this table

    HapMap Linkage Disequilibrium images for LASS4 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

      --

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    6 PubMed articles for LASS4 gene:
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 79603 HGNC: 23747 AceView: LASS4 Ensembl:ENSG00000090661 euGenes: HUgn79603
    ECgene: LASS4 H-InvDB: LASS4
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for LASS4:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



      Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
      Invitrogen Antibodies  Invitrogen Assays
      Invitrogen Clones  Invitrogen Q-PCR Products
      Invitrogen Human Recombinant Kinases  Invitrogen Custom Antibody and Peptide Service
      Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for LASS4-related products

     Millipore Custom Antibody & Bulk Services
     Millipore Preclinical / Clinical Development Services
     Millipore Immunoassay Services
     Millipore Target Screening & Profiling Services


     Predesigned and custom siRNAs for LASS4 Antibodies for LASS4
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for LASS4 Browse small molecules at Sigma-Aldrich
     "Your Favorite Gene" Pathwaysfeedback


      
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     Antibodies for LASS4

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