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LASP1 Gene

protein-coding   GIFtS: 60

GC17P034280
LIM and SH3 protein 1
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
LASP-1 3
Lasp-1 1, 2
MLN50 1, 2, 3, 5
Descriptions
LIM and SH3 protein 1 2
MLN 50 3
External Ids
HGNC: 65131
Entrez Gene: 39272
UniProtKB: Q148473
Ensembl: ENSG000000028347
Search outside databases for aliases for LASP1 gene

Previous GC identifers: GC17P036529 GC17P039024 GC17P036936 GC17P037401

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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EntrezGene summary for LASP1:
This gene encodes a member of a LIM protein subfamily characterized by a LIM motif and a domain of
Src homology region 3. The encoded protein functions as an actin-binding protein and possibly in
cytoskeletal organization. [provided by RefSeq]

UniProtKB/Swiss-Prot: LASP1_HUMAN, Q14847
Function: Plays an important role in the regulation of dynamic actin-based, cytoskeletal
activities. Agonist-dependent changes in LASP1 phosphorylation may also serve to regulate
actin-associated ion transport activities, not only in the parietal cell but also in certain other
F-actin-rich secretory epithelial cell types (By similarity)

Gene Wiki entry for LASP1

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the LASP1 gene  

Entrez Gene cytogenetic band: 17q11-q21.3   Ensembl cytogenetic band:  17q12   HGNC cytogenetic band: 17q11-q21.3

LASP1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 17         GeneLoc Exon Structure

GeneLoc location for GC17P034280:     (about GC identifiers)

Start:
34,279,894 bp from pter
End:
34,331,541 bp from pter
Size:
51,648 bases
Orientation:
plus strand
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: LASP1_HUMAN, Q14847 (See protein sequence)
Recommended Name: LIM and SH3 domain protein 1  
Size: 261 amino acids; 29717 Da
Subunit: Interacts with F-actin (By similarity)
Subcellular location: Cytoplasm, cell cortex (By similarity). Cytoplasm, cytoskeleton (By
similarity). Note=Associated with the F-actin rich cortical cytoskeleton (By similarity)
Secondary accessions: Q96ED2 Q96IG0
Alternative splicing: 2 isoforms:  Q14847-1   Q14847-2   

Post-translational modifications:

  • Phosphorylated (By similarity)1
  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_006139.1  

    ENSEMBL proteins: 
    ENSP00000325240 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (LASP1)
    Human Recombinant Proteins from Abnova (LASP1)
                  OriGene Purified Recombinant Human Protein: LASP1 

    4 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005737 cytoplasm IEA--
    GO:0005856 cytoskeleton IEA--
    GO:0005925 focal adhesion IEA--
    GO:0030864 cortical actin cytoskeleton ISS--
    About this table

    Antibodies for LASP1: 
    Invitrogen Antibodies for LASP1
    Millipore Mono- and Polyclonal Antibodies for the study of LASP1
    Sigma-Aldrich Antibodies for LASP1
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (LASP1), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (LASP1)
    Novus Biologicals Antibodies for LASP1

    Assays for LASP1: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    4 InterPro domains/families:
     IPR000900 Nebulin_35r-motif
     IPR001781 Znf_LIM
     IPR013998 Nebulin
     IPR001452 SH3_domain


       GeneDecks  LASP1 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q14847

    ProtoNet protein and cluster: Q14847

    2 Blocks protein families:
    IPB001452 SH3 domain signature
    IPB001781 Zn-binding protein


    UniProtKB/Swiss-Prot: LASP1_HUMAN, Q14847
    Similarity: Contains 1 LIM zinc-binding domain
    Similarity: Contains 2 nebulin repeats
    Similarity: Contains 1 SH3 domain

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (LASP1)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (LASP1)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_006148

                  Applied Biosystems Silencer® siRNAs for LASP1

                  Sigma-Aldrich siRNA for LASP1  
                         Sigma-Aldrich shRNA Panels and shRNA for LASP1  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for LASP1
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_006148
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_006148
                                     untagged cDNA clone in CMV expression vector: NM_006148 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_006148

    UniProtKB/Swiss-Prot: LASP1_HUMAN, Q14847
    Function: Plays an important role in the regulation of dynamic actin-based, cytoskeletal
    activities. Agonist-dependent changes in LASP1 phosphorylation may also serve to regulate
    actin-associated ion transport activities, not only in the parietal cell but also in certain other
    F-actin-rich secretory epithelial cell types (By similarity)

    Genatlas biochemistry entry for LASP1:
    LIM and SH3 protein 1,actin binding protein,amplified in breast cancer,metastatic axillary lymph
    nodes,cDNA50,potentially involved in a signaling pathway for the organization of the cytoskeleton

    6 MGI mutant phenotypes (inferred from 1 allele(MGI details for Lasp1):

    digestive/alimentaryendocrine/exocrine glandgrowth/sizereproductive system
    skeleton

    5/6 Gene Ontology (GO) molecular function terms (links to tree view) (see all 6 ):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005070 SH3/SH2 adaptor activity TAS7589475
    GO:0005515 protein binding IEA--
    GO:0008270 zinc ion binding IEA--
    GO:0015075 ion transmembrane transporter activity ISS--
    GO:0046872 metal ion binding IEA--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


    1 Millipore Pathway for LASP1
     Cytoskeleton remodeling Role of PKA in cytoskeleton reorganisation

       GeneDecks  LASP1 for the pathways selected above  
    About GeneDecksing

     Gene Network CentralTM Interacting Genes and Proteins Network for  LASP1 


    4 Interacting proteins for LASP1 (Q148472) via UniProtKB, MINT, and/or STRING
    InteractantInteraction Details
    GeneCardExternal ID(s)
    MDFIQ997502MINT-67947
    PLSCR1O151622MINT-66313
    TRIP13Q156452MINT-66300
    --Q9BVA22MINT-67591
    About this table

    2 Gene Ontology (GO) biological process terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006811 ion transport ISS--
    GO:0030865 cortical cytoskeleton organization IEA--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for LASP1

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (LASP1)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (LASP1)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_006148

                  Sigma-Aldrich siRNA for LASP1  
                         Sigma-Aldrich shRNA Panels and shRNA for LASP1  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_006148  

    REFSEQ mRNAs for LASP1 gene: 

    NM_006148.2   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_006148  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_006148
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_006148
                                     untagged cDNA clone in CMV expression vector: NM_006148 

    24/984 AceView cDNA sequences (see all 984 ):

    BM694929 CB125284 AI869239 BQ023027 AA834479 AI769037 BM830011 AI291491 
    CB120048 BP362580 BM783295 T61881 BM766838 BM746502 AI568122 AA043214 
    CR621995 AI435882 BM849660 BM786583 AW860349 BM795228 BM996225 AI754080 

    highest scoring ESTs for LASP1:

    X82456 AA022732 AA022824 AA035778 AA043214 AA043215 AA056356 AA074317 AA102155 AA121101 


    GeneLoc Exon Structure

    5/8 Alternative Splicing Database (ASD) splice patterns (SP) for LASP1 (see all 8 )

    ExUns: 1a · 1b ^ 2 ^ 3 ^ 4 ^ 5a · 5b ^ 6 ^ 7a · 7b ^ 8 ^ 9a · 9b · 9c ^ 10a · 10b ^ 11 ^ 12 ^ 13
    SP1:                    -           -           -                                                                     
    SP2:                                -           -                                                                     
    SP3:                    -           -     -     -                                                                     
    SP4:                                -           -           -     -     -                                             
    SP5:                                            -                                                                     

    About this scheme

    ECgene alternative splicing isoforms for LASP1

    1 Ensembl transcript including schematic representation:
    ENST00000318008  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    LASP1 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for LASP1

    1 / 2 / 3

    3 probe-sets matching LASP1 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

    36181_at2, 3 U95-A 1 1.00 1.00 1.00 1.00 X82456 1.00 0.90 0.95 1

    200618_at2, 3 U133-A 1 1.00 1.00 -- -- NM_006148 0.60 0.83 0.73 1

    200618_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    About this table
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: CACTTTTGGG

    Expression variation in blood from EXPOLDB for LASP1

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for LASP1 gene from 5/11 species (see all 11 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    LASP11   -- LIM and SH3 protein 1 92.45(n)
    96.48(a)
    608624  XM_846621.1  XP_851714.1 
    chimpanzee
    (Pan troglodytes)
    LASP11   -- LIM and SH3 protein 1 99.36(n)
    100(a)
    454620  XM_511445.2  XP_511445.2 
    cow
    (Bos taurus)
    LASP11   -- LIM and SH3 protein 1 93.08(n)
    96.15(a)
    532851  NM_001035394.1  NP_001030471.1 
    rat
    (Rattus norvegicus)
    Lasp11   -- LIM and SH3 protein 1 90.93(n)
    96.93(a)
    29278  NM_032613.2  NP_116002.1 
    mouse
    (Mus musculus)
    Lasp11, 5 11 (56.00 cM)5
    LIM and SH3 protein 11, 5 91.19(n)1
    96.17(a)1
    167961  NM_010688.31  NP_034818.11 
     AA4086295  AK0344045  (see all 31)
    About this table        Species with no ortholog for LASP1

    ENSEMBL Gene Tree for LASP1
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for LASP1 gene
    NRAP2  NEB2  NEBL2  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/361 NCBI SNPs in LASP1 are shown (see all 361 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 139)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 17 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs6604131,2
    A,C,F,H,O34329032(-) TGGTCT/CCTACA 1 -- ut3110Minor allele frequency- C:0.26EA NA EU WA 602
    --
    rs6460971,2
    A,C,F34329857(-) TCCTCT/CACTCA 1 -- ut31 ese34Minor allele frequency- C:0.31EA NA MN 236
    --
    rs80677441,2
    A,H34330860(+) ACCCCC/TAGAGA 1 -- ut31 ese34Minor allele frequency- T:0.00EU EA WA 420
    --
    rs22410101,2
    A,C34279665(-) GTGCTA/GGCGCC 1 -- ng510--------
    --
    rs20872941,2
    A,C34278974(+) cttttC/Tttttt 1 -- ng51 trp30--------
    --
    rs19628101,2
    A,C34279663(-) GCTGGC/TGCCTC 1 -- ng510--------
    rs115398451,2
    C,F34329284(+) TTTAGA/GGACAG 1 -- ut315Minor allele frequency- N:0.00NA EA MN 400
    rs80761531,2
    C34279762(+) CGCGCC/TCGAGG 1 -- ng510--------
    --
    rs38890671,2
    C34278975(-) aaaaaA/Gaaaaa 1 -- ng510--------
    rs72201141,2
    H34331822(+) GCTGAG/AAACTC 1 -- ng314Minor allele frequency- A:0.00EU EA WA 420
    About this table

    HapMap Linkage Disequilibrium images for LASP1 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, Novoseek, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 602920

    1 Novoseek disease relationship for LASP1 gene

    Disease   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
    breast cancer 37.52 7 16430883 (2), 12883659 (1), 17211471 (1), 7589475 (1) (see all 5)
    About this table

    1 PharmGKB disease relationship for LASP1 gene
    Disease PharmGKB Relations PubMed IDs for articles supporting these relationships
    Leukemia, Lymphocytic, Acute, L1FA  GN  12086872
    About this table

    Human Genome Epidemiology Navigator: LASP1 (1 document)

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/26 PubMed articles for LASP1 gene (see all 26 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 3927 HGNC: 6513 AceView: LASP1andPPP1R1B Ensembl:ENSG00000002834 euGenes: HUgn3927
    ECgene: LASP1 H-InvDB: LASP1
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

    NameDescription
    ATLAS Chromosomes in Cancer entry for LASP1 Genetics and Cytogenetics in Oncology and Haematology
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for LASP1:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



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     Predesigned and custom siRNAs for LASP1 Antibodies for LASP1
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for LASP1 Browse small molecules at Sigma-Aldrich
     "Your Favorite Gene" Pathwaysfeedback


      
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     Recombinant Proteins
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     Tagged/untagged cDNA clones
     Validated SYBR primer pairs
     Purified proteins from HEK293T cells
     Western blot validated antibodies
     shRNA in GFP-retroviral vector
     Search Tocris compounds for LASP1




     Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
     Antibodies for LASP1

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