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LARS2 Gene

protein-coding   GIFtS: 65

GC03P045405
leucyl-tRNA synthetase 2, mitochondrial
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
EC 6.1.1.4 3
KIAA0028 1, 2, 3
LEURS 1, 2
LeuRS 3
MGC26121 1, 2
Descriptions
Leucine--tRNA ligase 3
leucine tRNA ligase 2, mitocondrial 2
leucine translase 2
leucine-tRNA ligase 2
leucyl-tRNA synthetase 2, mitochondrial 2
External Ids
HGNC: 170951
Entrez Gene: 233952
UniProtKB: Q150313
Ensembl: ENSG000000113767
Search outside databases for aliases for LARS2 gene

Previous GC identifers: GC03P044670 GC03P045249 GC03P045390

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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EntrezGene summary for LARS2:
This gene encodes a class 1 aminoacyl-tRNA synthetase, mitochondrial leucyl-tRNA synthetase. Each
of the twenty aminoacyl-tRNA synthetases catalyzes the aminoacylation of a specific tRNA or tRNA
isoaccepting family with the cognate amino acid. [provided by RefSeq]

Gene Wiki entry for LARS2
(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
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Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the LARS2 gene  

Entrez Gene cytogenetic band: 3p21.3   Ensembl cytogenetic band:  3p21.31   HGNC cytogenetic band: 3p21.3

LARS2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 3         GeneLoc Exon Structure

GeneLoc location for GC03P045405:     (about GC identifiers)

Start:
45,405,072 bp from pter
End:
45,565,332 bp from pter
Size:
160,261 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000003.10  NT_022517.17  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: SYLM_HUMAN, Q15031 (See protein sequence)
Recommended Name: Probable leucyl-tRNA synthetase, mitochondrial precursor  
Size: 903 amino acids; 101976 Da
Subcellular location: Mitochondrion matrix (By similarity)

REFSEQ proteins: NP_056155.1  

ENSEMBL proteins: 
ENSP00000265537 


Human Recombinant Proteins 
Browse Drug Discovery Central at Invitrogen for human recombinant proteins
Browse Purified and Recombinant Proteins at Millipore
Browse Human Recombinant Proteins at Sigma-Aldrich  
Browse R&D Systems for human recombinant proteins
Browse recombinant and purified proteins available from Enzo Life Sciences
Recombinant Proteins from Abcam (LARS2)
Human Recombinant Proteins from Abnova (LARS2)
                Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

3 Gene Ontology (GO) cellular component terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005737 cytoplasm IEA--
GO:0005739 mitochondrion IEA--
GO:0005759 mitochondrial matrix IEA--
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Antibodies for LARS2: 
Browse Antibodies Central at Invitrogen
Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
Sigma-Aldrich Antibodies for LARS2
Browse R&D Systems for Antibodies
Antibodies from Abcam (LARS2), each with their AbpromiseSM
Monoclonal and Polyclonal Antibodies from Abnova (LARS2)
Novus Biologicals Antibodies for LARS2

Assays for LARS2: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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5/6 InterPro domains/families (see all 6 ):
 IPR001412 aa-tRNA-synth_I_CS
 IPR015413 aa-tRNA-synt_I
 IPR014729 Rossmann-like_a/b/a_fold
 IPR002302 Leu-tRNA-synth_Ia_bac/mito
 IPR013155 V/L/I-tRNA-synth_anticodon-bd


   GeneDecks  LARS2 for the domains selected above  
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Graphical View of Domain Structure for InterPro Entry Q15031

ProtoNet protein and cluster: Q15031

2 Blocks protein families:
IPB002300 Aminoacyl-tRNA synthetase
IPB002302 Leucyl-tRNA synthetase signature


UniProtKB/Swiss-Prot: SYLM_HUMAN, Q15031
Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (LARS2)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (LARS2)
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_015340

              Applied Biosystems Silencer® siRNAs for LARS2

              Sigma-Aldrich siRNA and siRNA Panels for LARS2  
                     Sigma-Aldrich shRNA for LARS2  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Invitrogen Clones for LARS2
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_015340
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_015340
                                 untagged cDNA clone in CMV expression vector: NM_015340 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_015340

UniProtKB/Swiss-Prot: SYLM_HUMAN, Q15031
Catalytic activity: ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu)
Enzyme Number (IUBMB): EC 6.1.1.4 

4 Gene Ontology (GO) molecular function terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0000166 nucleotide binding IEA--
GO:0004823 leucine-tRNA ligase activity IEA--
GO:0005524 ATP binding IEA--
GO:0016874 ligase activity IEA--
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(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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2 Sigma-Aldrich "Your Favorite Gene" Pathways for  LARS2  (Your Favorite Gene powered by Ingenuity) 
 Aminoacyl-tRNA Biosynthesis
 Valine, Leucine and Isoleucine Biosynthesis

   GeneDecks  LARS2 for the pathways selected above  
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2 Kegg Pathways  (Kegg details for LARS2):
 hsa00290 Valine, leucine and isoleucine biosynthesis
 hsa00970 Aminoacyl-tRNA biosynthesis

   GeneDecks  LARS2 for the pathways selected above  
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5 Interacting proteins for LARS2 (ENSP000002655373) via UniProtKB, MINT, and/or STRING
InteractantInteraction Details
GeneCardExternal ID(s)
LIMD1ENSP000002733173STRING (score=.936)
BCAT1ENSP000002611923STRING (score=.899)
BCAT2ENSP000003229913STRING (score=.899)
LARSENSP000002745623STRING (score=.884)
FAM50AENSP000001585263STRING (score=.853)
About this table

1 Gene Ontology (GO) biological process term (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0006429 leucyl-tRNA aminoacylation IEA--
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(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for LARS2
1 Novoseek chemical compound relationship for LARS2 gene
Compound   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
trna 65.57 10 17378584 (1), 15723544 (1), 12549915 (1), 19351898 (1)
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(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (LARS2)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (LARS2)
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_015340

              Sigma-Aldrich siRNA and siRNA Panels for LARS2  
                     Sigma-Aldrich shRNA for LARS2  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_015340  

REFSEQ mRNAs for LARS2 gene: 

NM_015340.3   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_015340  

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_015340
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_015340
                                 untagged cDNA clone in CMV expression vector: NM_015340 

Additional cDNA sequence: 

AK025465.1 AK026113.1 AK308146.1 BC025989.2 CR620253.1 D21851.1 

5 DOTS entries:

DT.452983  DT.97788299  DT.120879587  DT.120879610  DT.91738877 

24/135 AceView cDNA sequences (see all 135 ):

BE464286 BU730560 AK026113 BM982045 BM471576 CA439386 BU609039 BM564210 
NM_015340 CR620253 CA431425 AI361703 BQ226824 AI658894 AW087297 AL599220 
BU184816 BM927807 AW075872 BG286778 D21851 AA489504 AI352357 BQ649567 

highest scoring ESTs for LARS2:

D21851 AA080981 AA312721 AA482999 AA489504 AA564414 AA688001 AA877570 AA884360 AA969386 

Unigene Cluster for LARS2:

Leucyl-tRNA synthetase 2, mitochondrial
Hs.526975  [show with all ESTs]
Unigene Representative Sequence: BC025989


GeneLoc Exon Structure

4 Alternative Splicing Database (ASD) splice patterns (SP) for LARS2

ExUns: 1a · 1b ^ 2 ^ 3 ^ 4 ^ 5a · 5b ^ 6 ^ 7 ^ 8 ^ 9 ^ 10 ^ 11 ^ 12 ^ 13 ^ 14 ^ 15 ^ 16 ^ 17 ^ 18 ^ 19 ^ 20 ^ 21 ^ 22a · 22b ^ 23a ·
SP1:              -                                                                                                                             -               
SP2:                                                                                                                                                            
SP3:              -     -                                                                                                                                       
SP4:                                                                                                                                                            

ExUns: 23b
SP1:      
SP2:      
SP3:      
SP4:      

About this scheme

ECgene alternative splicing isoforms for LARS2

1 Ensembl transcript including schematic representation:
ENST00000265537  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
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LARS2 expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for LARS2

1 / 2 / 3

5 probe-sets matching LARS2 gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

34764_at2, 3 U133-A 1 1.00 1.00 1.00 1.00 D21851 1.00 1.00 1.00 1

34764_at2, 3 U133-A 1 1.00 1.00 -- -- D21851 1.00 1.00 1.00 1

204016_at2, 3 U133-A 1 1.00 1.00 -- -- NM_015340 0.60 1.00 0.82 1

34764_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

204016_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
About this table
Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: TGGTCCCCCT

SOURCE GeneReport for Unigene cluster: Hs.526975

Expression variation in blood from EXPOLDB for LARS2

(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for LARS2 gene from 5/19 species (see all 19 )
Organism Gene Locus Description Human
Similarity
NCBI accessions
dog
(Canis familiaris)
LARS21   -- leucyl-tRNA synthetase 2, mitochondrial 88.04(n)
90.48(a)
476652  XM_533856.2  XP_533856.2 
chimpanzee
(Pan troglodytes)
LARS21   -- leucyl-tRNA synthetase 2, mitochondrial 99.34(n)
99.67(a)
739246  XM_001147524.1  XP_001147524.1 
rat
(Rattus norvegicus)
Lars21   -- leucyl-tRNA synthetase 2, mitochondrial 84.7(n)
87.03(a)
363172  XM_343511.3  XP_343512.2 
mouse
(Mus musculus)
Lars21, 5 9 (70.60 cM)5
leucyl-tRNA synthetase, mitochondrial1, 5 84.89(n)1
86.59(a)1
1024361  NM_153168.21  NP_694808.11 
 AI0355465  AJ4280665  (see all 19)
chicken
(Gallus gallus)
LARS21   -- leucyl-tRNA synthetase 2, mitochondrial 71.1(n)
70.99(a)
420702  XM_418801.2  XP_418801.1 
About this table        Species with no ortholog for LARS2

ENSEMBL Gene Tree for LARS2
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

  --
(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
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10/841 NCBI SNPs in LARS2 are shown (see all 841 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 267)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 3 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
rs8640951,2
A,C,F,H45404490(+) GCCGGG/CTGTGT 1 -- ng517Minor allele frequency- C:0.35MN EU EA WA NA 782
rs2672661,2
C,F,H45564415(+) TAGGCG/ACTTCT 1 -- ut31 ese316Minor allele frequency- A:0.12NA EA MN EU WA 1431
rs98276891,2
C,F,H45558413(+) CCGGAG/TTTCCT 1 E/D mis1 ese39Minor allele frequency- T:0.02EU EA WA NA 906
rs37558251,2
C,F,H45404687(-) TGACTG/TCATGA 1 -- ng519Minor allele frequency- T:0.24EA MN EU WA NA 2752
rs2672671,2
C,F,H45564706(-) GGCACG/ATCCAG 1 -- ut317Minor allele frequency- A:0.14NA EA EU WA 671
rs8541871,2
C,F,H45404895(+) TTTGCC/AGACAC 1 -- ng5112Minor allele frequency- A:0.12MN EA EU WA NA 3118
rs360542301,2
C,F45534535(+) AGGAAG/TCTCTG 1 K/N mis15Minor allele frequency- T:0.02NA EU EA WA 498
rs284131881,2
F45405155(+) GAGCCG/ACAGGC 1 -- ut51 ese31Minor allele frequency- A:0.08EA 852
rs21283611,2
A,C,F,H,O45508228(-) AAAGAT/CGCGAT 1 A/A syn112Minor allele frequency- C:0.21NA EU EA WA 1058
rs76103571,2
A,C,F,H45502222(+) ATGCTC/TACCCA 1 L/L syn1 ese35Minor allele frequency- T:0.22NA EU EA WA 488
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HapMap Linkage Disequilibrium images for LARS2 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 604544

Human Gene Mutation Database: LARS2
Genetic Association Database: LARS2
Human Genome Epidemiology Navigator: LARS2 (1 document)

(Possibly Related Articles in Doctor's Guide)
About This Section

  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
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10/22 PubMed articles for LARS2 gene (see all 22 ):
(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 23395 HGNC: 17095 AceView: LARS2 Ensembl:ENSG00000011376 euGenes: HUgn23395
ECgene: LARS2 H-InvDB: LARS2
(According to HUGE)
About This Section

HUGE: KIAA0028
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

  --
(Available from WIS Yeda, Salk, Tufts)
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  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for LARS2:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



  Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
  Invitrogen Antibodies  Invitrogen Assays
  Invitrogen Clones  Invitrogen Q-PCR Products
  Invitrogen Human Recombinant Kinases  Invitrogen Custom Antibody and Peptide Service
  Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for LARS2-related products

 Millipore Custom Antibody & Bulk Services
 Millipore Preclinical / Clinical Development Services
 Millipore Immunoassay Services
 Millipore Target Screening & Profiling Services


 Predesigned and custom siRNAs for LARS2 Antibodies for LARS2
 Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
 Lentivirus-delivered shRNAs for LARS2 Browse small molecules at Sigma-Aldrich
 "Your Favorite Gene" Pathwaysfeedback


  
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 Recombinant & Natural Proteins  

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 Recombinant Proteins
(LARS2)
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 Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
 Antibodies for LARS2

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